Development of real-time RT-PCR systems for detection and quantitation of bovine enteric viral pathogens
In conclusion, real time PCR developed for these viruses are highly specific and sensitive technique for the detection of diarrheic viral pathogens of cattle and buffalo.PMID:38347693 | DOI:10.1080/10495398.2023.2182314 (Source: Animal Biotechnology)
Source: Animal Biotechnology - February 13, 2024 Category: Biotechnology Authors: Monika Punia Sushila Maan Kanisht Batra Deepika Chaudhary Bhanita Devi Aman Kumar Suresh Kumar Gahlawat Narender Singh Maan Source Type: research
Development of real-time RT-PCR systems for detection and quantitation of bovine enteric viral pathogens
In conclusion, real time PCR developed for these viruses are highly specific and sensitive technique for the detection of diarrheic viral pathogens of cattle and buffalo.PMID:38347693 | DOI:10.1080/10495398.2023.2182314 (Source: Animal Biotechnology)
Source: Animal Biotechnology - February 13, 2024 Category: Biotechnology Authors: Monika Punia Sushila Maan Kanisht Batra Deepika Chaudhary Bhanita Devi Aman Kumar Suresh Kumar Gahlawat Narender Singh Maan Source Type: research
Development of real-time RT-PCR systems for detection and quantitation of bovine enteric viral pathogens
In conclusion, real time PCR developed for these viruses are highly specific and sensitive technique for the detection of diarrheic viral pathogens of cattle and buffalo.PMID:38347693 | DOI:10.1080/10495398.2023.2182314 (Source: Animal Biotechnology)
Source: Animal Biotechnology - February 13, 2024 Category: Biotechnology Authors: Monika Punia Sushila Maan Kanisht Batra Deepika Chaudhary Bhanita Devi Aman Kumar Suresh Kumar Gahlawat Narender Singh Maan Source Type: research
Study of Shellfish Growing Area During Normal Harvesting Periods and Following Wastewater Overflows in an Urban Estuary With Complex Hydrography
In this study, norovirus genogroup I and II, indicators of viral contamination (F-RNA bacteriophage genogroup II (F-RNA GII), crAssphage, pepper mild mottle virus) andEscherichia coli were monitored during periods of normal harvesting and following overflows in two commercial shellfish growing areas in Otago Harbour (Aotearoa New Zealand). Dye tracing, drogue tracking and analysis of particle tracking modelling were also undertaken to assess the dispersion, dilution and time of travel of wastewater discharged from a pump station discharge that impacts the growing areas. Norovirus was not detected in any of the 218 shellfis...
Source: Food and Environmental Virology - February 8, 2024 Category: Virology Source Type: research
A DNA vaccine against GII.4 human norovirus VP1 induces blocking antibody production and T cell responses
In this study, we constructed a CMV/R vector expressing a major structural protein, VP1, of GII.4 HuNoV (CMV/R-GII.4 HuNoV VP1). Transfection of CMV/R-GII.4 HuNoV VP1 into human embryonic kidney 293T (HEK293T) cells resulted in successful expression of VP1 proteins in vitro. Intramuscular or intradermal immunization of mice with the CMV/R-GII.4 HuNoV VP1 construct elicited the production of blocking antibodies and activation of T cell responses against GII.4 HuNoV VP1. Our collective data support the utility of CMV/R-GII.4 HuNoV VP1 as a promising DNA vaccine candidate against GII.4 HuNoV.PMID:38320930 | DOI:10.1016/j.vacc...
Source: Vaccine - February 6, 2024 Category: Allergy & Immunology Authors: Na-Eun Kim Mun-Jin Kim Bum Ju Park Jung Won Kwon Jae Myun Lee Jung-Hwan Park Yoon-Jae Song Source Type: research
Viruses, Vol. 16, Pages 241: Replication of Human Norovirus in Human Intestinal Enteroids Is Affected by Fecal Sample Processing
The objective of this study was to investigate whether serially centrifuged fecal samples, RNA extraction kit (QIAamp versus MagMaX) and HIE age (newer versus older) affect HuNoV RNA fold increase in HIE. HuNoV GII.1, GII.4 and GII.6 fecal samples were prepared by serial centrifugation and filtration and the viral RNA in HIE was quantified at 1 and 72 h post-infection (hpi) following RNA extraction and RT-qPCR. The serially filtered GII.1, GII.4 and GII.6 showed successful replication in HIE, resulting in mean log increases of 2.2, 2 and 1.2, respectively, at 72 vs. 1 hpi. In contrast, only serially centrifuged GII.1 showe...
Source: Viruses - February 2, 2024 Category: Virology Authors: Revati Narwankar Malak A. Esseili Tags: Article Source Type: research
Human intestinal enteroids and predictive models validate the operational limits of sanitizers used for viral disinfection of vegetable process wash water
This study aimed to explore the disinfection efficacy of chlorine (free chlorine, FC), chlorine dioxide (ClO2) and peracetic acid (PAA) in PWW against infectious human norovirus and Tulane virus (TV). First, we tested the extent of TV inactivation in baby leaf, bell pepper, and vegetables mix PWW and monitored the viral decay by cell culture. Then, inactivation kinetics were defined for infectious human norovirus exposed to FC, ClO2 and PAA in baby leaves PWW using the human intestinal enteroids (HIE) system. Finally, kinetic inactivation models were fitted to TV reduction and decay of sanitizers to aid the implementation ...
Source: International Journal of Food Microbiology - February 1, 2024 Category: Food Science Authors: Ana Allende Jos é Antonio Férez-Rubio Juan Antonio Tudela Rosa Aznar Maria Isabel Gil Gloria S ánchez Walter Randazzo Source Type: research
Heat stability of foodborne viruses - Findings, methodological challenges and current developments
Int J Food Microbiol. 2024 Mar 2;413:110582. doi: 10.1016/j.ijfoodmicro.2024.110582. Epub 2024 Jan 25.ABSTRACTHeat treatment of food represents an important measure to prevent pathogen transmission. Thus far, evaluation of heat treatment processes is mainly based on data from bacteria. However, foodborne viruses have gained increasing attention during the last decades. Here, the published literature on heat stability and inactivation of human norovirus (NoV), hepatitis A virus (HAV) and hepatitis E virus (HEV) was reviewed. Data for surrogate viruses were not included. As stability assessment for foodborne viruses is often...
Source: International Journal of Food Microbiology - January 30, 2024 Category: Food Science Authors: Reimar Johne Johannes Scholz Alexander Falkenhagen Source Type: research
Prevalence, Clinical Features, and Genotypes of Norovirus-Associated Diarrhea in Wuxi, China, 2013-2020
This study aimed to determine the prevalence, clinical features, and genotypes of NoV-associated diarrhea in Wuxi, China. A total of 4,416 stool samples were collected from patients with diarrhea at enteric disease clinics of sentinel hospitals in Wuxi from February 1, 2013 to December 31, 2020. Univariate and Akaike information criterion stepwise logistic regression were used to identify differences as integrated within a clinical setting (NoV positive [+] versus NoV negative [-], NoV+ versus rotavirus [RV]+, NoV+ versus bacteria+, genogroup [G] I and GII genotypes). Norovirus was detected in 9.85% of stool samples, which...
Source: Am J Trop Med Hyg - January 24, 2024 Category: Infectious Diseases Authors: Yan Wang Yumeng Gao Chao Shi Yuan Shen Mingyan Lu Dan Sha Yujun Chen Ding Zhu Ping Shi Source Type: research
Prevalence, Clinical Features, and Genotypes of Norovirus-Associated Diarrhea in Wuxi, China, 2013-2020
This study aimed to determine the prevalence, clinical features, and genotypes of NoV-associated diarrhea in Wuxi, China. A total of 4,416 stool samples were collected from patients with diarrhea at enteric disease clinics of sentinel hospitals in Wuxi from February 1, 2013 to December 31, 2020. Univariate and Akaike information criterion stepwise logistic regression were used to identify differences as integrated within a clinical setting (NoV positive [+] versus NoV negative [-], NoV+ versus rotavirus [RV]+, NoV+ versus bacteria+, genogroup [G] I and GII genotypes). Norovirus was detected in 9.85% of stool samples, which...
Source: The American Journal of Tropical Medicine and Hygiene - January 24, 2024 Category: Tropical Medicine Authors: Yan Wang Yumeng Gao Chao Shi Yuan Shen Mingyan Lu Dan Sha Yujun Chen Ding Zhu Ping Shi Source Type: research
Meta-analysis of the prevalence of norovirus and hepatitis a virus in berries
Int J Food Microbiol. 2024 Mar 2;413:110577. doi: 10.1016/j.ijfoodmicro.2024.110577. Epub 2024 Jan 17.ABSTRACTNorovirus (NoV) and hepatitis A virus (HAV) stand as the predominant agents associated with viral foodborne infections. Outbreaks have been documented to be caused by various types of food items, including fresh and/or frozen berries. Comprehensive data concerning crucial viral pathogens in berries remain limited and are not currently available in aggregate form. Consequently, the present study aimed to compile the existing information regarding the prevalence of NoV and HAV in this matrix. Records of foodborne vir...
Source: International Journal of Food Microbiology - January 23, 2024 Category: Food Science Authors: Camila Miotti Marcelo Lisandro Signorini Juan Mart ín Oteiza Ver ónica Emilse Prez Patricia Ang élica Barril Source Type: research
Impact of the COVID-19 pandemic on norovirus circulation in Germany
Int J Med Microbiol. 2024 Jan 18;314:151600. doi: 10.1016/j.ijmm.2024.151600. Online ahead of print.ABSTRACTHuman norovirus is a major cause of viral gastroenteritis in all age groups. The virus is constantly and rapidly changing, allowing mutations and recombination events to create great diversity of circulating viruses. With the start of the COVID-19 pandemic in 2020, a wide range of public health measures were introduced worldwide to control human-to-human transmission of SARS-CoV-2. In Germany, control measures such as distance rules, contact restrictions, personal protection equipment as well as intensive hand hygien...
Source: International Journal of Medical Microbiology - January 21, 2024 Category: Microbiology Authors: Sonja Jacobsen Mirko Faber Britta Altmann Andreas Mas Marques C-Thomas Bock Sandra Niendorf Source Type: research
Viruses, Vol. 16, Pages 151: Aerosol Transmission of Norovirus
iyong Gao
Norovirus (NoV) is a major cause of acute gastroenteritis outbreaks worldwide. A comprehensive understanding of the transmission mode is of great significance for the prevention and control of the NoV infection. Currently, the transmission modes of NoV include contact, food-borne, water-borne and aerosol transmission. The first three modes are more common, while aerosol transmission is seldom reported. In this paper, the source, generation mechanism, infectivity, sampling and related outbreaks of NoV aerosol are summarized and discussed. (Source: Viruses)
Source: Viruses - January 19, 2024 Category: Virology Authors: Mengdi Tan Yi Tian Daitao Zhang Quanyi Wang Zhiyong Gao Tags: Review Source Type: research
An unusual diarrheal outbreak in the community in Eastern Thailand caused by Norovirus GII.3[P25]
Sentinel laboratory surveillance for diarrheal disease determined norovirus to be the most common cause of non-bacterial gastroenteritis in people during the COVID-19 pandemic in Thailand. An increase in patie... (Source: Virology Journal)
Source: Virology Journal - January 19, 2024 Category: Virology Authors: Patpong Udompat, Krongkan Srimuang, Pawinee Doungngern, Nattakarn Thippamom, Sininat Petcharat, Khwankamon Rattanatumhi, Sirorat Khiewbanyang, Pantila Taweewigyakarn, Somkid Kripattanapong, Sasiprapa Ninwattana, Ananporn Supataragul, Spencer L. Sterling, Tags: Research Source Type: research
Comprehensive full genome analysis of norovirus strains from eastern India, 2017 –2021
ConclusionThis study highlights the circulation of diverse norovirus strains in eastern India. These findings are important for understanding norovirus epidemiology in India and may have implications for future vaccine development. (Source: Gut Pathogens)
Source: Gut Pathogens - January 18, 2024 Category: Microbiology Source Type: research
