A digital droplet PCR detection technique based on filter faster R-CNN
CONCLUSION: The ddPCR detection technique using the Filter Faster R-CNN model provides a robust detection method for ddPCR under various environmental conditions.PMID:38501420 | PMC:PMC10954537 | DOI:10.12122/j.issn.1673-4254.2024.02.17 (Source: Journal of Southern Medical University)
Source: Journal of Southern Medical University - March 19, 2024 Category: Universities & Medical Training Authors: Y Zhang B Chen J Li Y Liang H Zhang W Wu Y Zhang Source Type: research
A digital droplet PCR detection technique based on filter faster R-CNN
CONCLUSION: The ddPCR detection technique using the Filter Faster R-CNN model provides a robust detection method for ddPCR under various environmental conditions.PMID:38501420 | PMC:PMC10954537 | DOI:10.12122/j.issn.1673-4254.2024.02.17 (Source: Journal of Southern Medical University)
Source: Journal of Southern Medical University - March 19, 2024 Category: Universities & Medical Training Authors: Y Zhang B Chen J Li Y Liang H Zhang W Wu Y Zhang Source Type: research
A digital droplet PCR detection technique based on filter faster R-CNN
CONCLUSION: The ddPCR detection technique using the Filter Faster R-CNN model provides a robust detection method for ddPCR under various environmental conditions.PMID:38501420 | PMC:PMC10954537 | DOI:10.12122/j.issn.1673-4254.2024.02.17 (Source: Journal of Southern Medical University)
Source: Journal of Southern Medical University - March 19, 2024 Category: Universities & Medical Training Authors: Y Zhang B Chen J Li Y Liang H Zhang W Wu Y Zhang Source Type: research
A digital droplet PCR detection technique based on filter faster R-CNN
CONCLUSION: The ddPCR detection technique using the Filter Faster R-CNN model provides a robust detection method for ddPCR under various environmental conditions.PMID:38501420 | PMC:PMC10954537 | DOI:10.12122/j.issn.1673-4254.2024.02.17 (Source: Journal of Southern Medical University)
Source: Journal of Southern Medical University - March 19, 2024 Category: Universities & Medical Training Authors: Y Zhang B Chen J Li Y Liang H Zhang W Wu Y Zhang Source Type: research
A digital droplet PCR detection technique based on filter faster R-CNN
CONCLUSION: The ddPCR detection technique using the Filter Faster R-CNN model provides a robust detection method for ddPCR under various environmental conditions.PMID:38501420 | PMC:PMC10954537 | DOI:10.12122/j.issn.1673-4254.2024.02.17 (Source: Journal of Southern Medical University)
Source: Journal of Southern Medical University - March 19, 2024 Category: Universities & Medical Training Authors: Y Zhang B Chen J Li Y Liang H Zhang W Wu Y Zhang Source Type: research
A digital droplet PCR detection technique based on filter faster R-CNN
CONCLUSION: The ddPCR detection technique using the Filter Faster R-CNN model provides a robust detection method for ddPCR under various environmental conditions.PMID:38501420 | PMC:PMC10954537 | DOI:10.12122/j.issn.1673-4254.2024.02.17 (Source: Journal of Southern Medical University)
Source: Journal of Southern Medical University - March 19, 2024 Category: Universities & Medical Training Authors: Y Zhang B Chen J Li Y Liang H Zhang W Wu Y Zhang Source Type: research
Evaluation of a new automated viral RNA extraction platform for hepatitis A virus and human norovirus in testing of berries, lettuce, and oysters
Int J Food Microbiol. 2024 Mar 7;416:110664. doi: 10.1016/j.ijfoodmicro.2024.110664. Online ahead of print.ABSTRACTFruits, vegetables, and shellfish are often associated with outbreaks of illness caused particularly by human norovirus (HuNoV) and hepatitis A virus (HAV), the leading causative agents of foodborne illness worldwide. The aim of this study was to evaluate a new automated nucleic acid extraction platform (EGENE-UP EASYPREP) for enteric viruses in several at-risk food matrices and to test its limit of detection in comparison to a semi-automated method (EGENE-UP) using Boom methodology for nucleic acid extraction...
Source: International Journal of Food Microbiology - March 16, 2024 Category: Food Science Authors: Mathilde Trudel-Ferland Marie- Ève Collard Val érie Goulet-Beaulieu Eric Jubinville Fabienne Hamon Julie Jean Source Type: research
Evaluation of a new automated viral RNA extraction platform for hepatitis A virus and human norovirus in testing of berries, lettuce, and oysters
Int J Food Microbiol. 2024 Mar 7;416:110664. doi: 10.1016/j.ijfoodmicro.2024.110664. Online ahead of print.ABSTRACTFruits, vegetables, and shellfish are often associated with outbreaks of illness caused particularly by human norovirus (HuNoV) and hepatitis A virus (HAV), the leading causative agents of foodborne illness worldwide. The aim of this study was to evaluate a new automated nucleic acid extraction platform (EGENE-UP EASYPREP) for enteric viruses in several at-risk food matrices and to test its limit of detection in comparison to a semi-automated method (EGENE-UP) using Boom methodology for nucleic acid extraction...
Source: International Journal of Food Microbiology - March 16, 2024 Category: Food Science Authors: Mathilde Trudel-Ferland Marie- Ève Collard Val érie Goulet-Beaulieu Eric Jubinville Fabienne Hamon Julie Jean Source Type: research
Evaluation of a new automated viral RNA extraction platform for hepatitis A virus and human norovirus in testing of berries, lettuce, and oysters
Int J Food Microbiol. 2024 Mar 7;416:110664. doi: 10.1016/j.ijfoodmicro.2024.110664. Online ahead of print.ABSTRACTFruits, vegetables, and shellfish are often associated with outbreaks of illness caused particularly by human norovirus (HuNoV) and hepatitis A virus (HAV), the leading causative agents of foodborne illness worldwide. The aim of this study was to evaluate a new automated nucleic acid extraction platform (EGENE-UP EASYPREP) for enteric viruses in several at-risk food matrices and to test its limit of detection in comparison to a semi-automated method (EGENE-UP) using Boom methodology for nucleic acid extraction...
Source: International Journal of Food Microbiology - March 16, 2024 Category: Food Science Authors: Mathilde Trudel-Ferland Marie- Ève Collard Val érie Goulet-Beaulieu Eric Jubinville Fabienne Hamon Julie Jean Source Type: research
Surveillance of Waterborne Disease Outbreaks Associated with Drinking Water - United States, 2015-2020
This report summarizes NORS drinking water outbreak epidemiologic, laboratory, and environmental data, including data for both public and private drinking water systems. The report presents outbreak-contributing factors (i.e., practices and factors that lead to outbreaks) and, for the first time, categorizes outbreaks as biofilm pathogen or enteric illness associated.PERIOD COVERED: 2015-2020.DESCRIPTION OF SYSTEM: CDC launched NORS in 2009 as a web-based platform into which public health departments voluntarily enter outbreak information. Through NORS, CDC collects reports of enteric disease outbreaks caused by bacterial,...
Source: MMWR Surveill Summ - March 12, 2024 Category: Epidemiology Authors: Jasen M Kunz Hannah Lawinger Shanna Miko Megan Gerdes Muhammad Thuneibat Elizabeth Hannapel Virginia A Roberts Source Type: research
Asymptomatic Enteric Virus Infections and Association with the Gut Microbiome in Rural Residents of Northern Laos
This study aimed to determine the prevalence of and factors associated with asymptomatic enteric virus infection in adults in northern Laos. Fecal samples were collected from apparently healthy participants who did not report diarrhea or high fever at the time of the survey in northern Laos, and enteric viruses were detected using polymerase chain reaction (PCR) and reverse transcription (RT)-PCR. Individual characteristics, including the gut microbiome, were compared between asymptomatic carriers and noncarriers of each enteric virus. Of the participants (N = 255), 12 (4.7%) were positive for norovirus genogroup I (GI), 8...
Source: The American Journal of Tropical Medicine and Hygiene - March 12, 2024 Category: Tropical Medicine Authors: Sae Sekiya Hiroaki Masuoka Yuki Mizuno Mihoko Kibe Satoko Kosaka Kazumi Natsuhara Kazuhiro Hirayama Nouhak Inthavong Sengchanh Kounnavong Shinsuke Tomita Lena Takayasu Wataru Suda Fumihiro Yagyu Masahiro Umezaki Source Type: research
Asymptomatic Enteric Virus Infections and Association with the Gut Microbiome in Rural Residents of Northern Laos
This study aimed to determine the prevalence of and factors associated with asymptomatic enteric virus infection in adults in northern Laos. Fecal samples were collected from apparently healthy participants who did not report diarrhea or high fever at the time of the survey in northern Laos, and enteric viruses were detected using polymerase chain reaction (PCR) and reverse transcription (RT)-PCR. Individual characteristics, including the gut microbiome, were compared between asymptomatic carriers and noncarriers of each enteric virus. Of the participants (N = 255), 12 (4.7%) were positive for norovirus genogroup I (GI), 8...
Source: Am J Trop Med Hyg - March 12, 2024 Category: Infectious Diseases Authors: Sae Sekiya Hiroaki Masuoka Yuki Mizuno Mihoko Kibe Satoko Kosaka Kazumi Natsuhara Kazuhiro Hirayama Nouhak Inthavong Sengchanh Kounnavong Shinsuke Tomita Lena Takayasu Wataru Suda Fumihiro Yagyu Masahiro Umezaki Source Type: research
Surveillance of Waterborne Disease Outbreaks Associated with Drinking Water - United States, 2015-2020
This report summarizes NORS drinking water outbreak epidemiologic, laboratory, and environmental data, including data for both public and private drinking water systems. The report presents outbreak-contributing factors (i.e., practices and factors that lead to outbreaks) and, for the first time, categorizes outbreaks as biofilm pathogen or enteric illness associated.PERIOD COVERED: 2015-2020.DESCRIPTION OF SYSTEM: CDC launched NORS in 2009 as a web-based platform into which public health departments voluntarily enter outbreak information. Through NORS, CDC collects reports of enteric disease outbreaks caused by bacterial,...
Source: MMWR Surveill Summ - March 12, 2024 Category: Epidemiology Authors: Jasen M Kunz Hannah Lawinger Shanna Miko Megan Gerdes Muhammad Thuneibat Elizabeth Hannapel Virginia A Roberts Source Type: research
Asymptomatic Enteric Virus Infections and Association with the Gut Microbiome in Rural Residents of Northern Laos
This study aimed to determine the prevalence of and factors associated with asymptomatic enteric virus infection in adults in northern Laos. Fecal samples were collected from apparently healthy participants who did not report diarrhea or high fever at the time of the survey in northern Laos, and enteric viruses were detected using polymerase chain reaction (PCR) and reverse transcription (RT)-PCR. Individual characteristics, including the gut microbiome, were compared between asymptomatic carriers and noncarriers of each enteric virus. Of the participants (N = 255), 12 (4.7%) were positive for norovirus genogroup I (GI), 8...
Source: The American Journal of Tropical Medicine and Hygiene - March 12, 2024 Category: Tropical Medicine Authors: Sae Sekiya Hiroaki Masuoka Yuki Mizuno Mihoko Kibe Satoko Kosaka Kazumi Natsuhara Kazuhiro Hirayama Nouhak Inthavong Sengchanh Kounnavong Shinsuke Tomita Lena Takayasu Wataru Suda Fumihiro Yagyu Masahiro Umezaki Source Type: research
Asymptomatic Enteric Virus Infections and Association with the Gut Microbiome in Rural Residents of Northern Laos
This study aimed to determine the prevalence of and factors associated with asymptomatic enteric virus infection in adults in northern Laos. Fecal samples were collected from apparently healthy participants who did not report diarrhea or high fever at the time of the survey in northern Laos, and enteric viruses were detected using polymerase chain reaction (PCR) and reverse transcription (RT)-PCR. Individual characteristics, including the gut microbiome, were compared between asymptomatic carriers and noncarriers of each enteric virus. Of the participants (N = 255), 12 (4.7%) were positive for norovirus genogroup I (GI), 8...
Source: Am J Trop Med Hyg - March 12, 2024 Category: Infectious Diseases Authors: Sae Sekiya Hiroaki Masuoka Yuki Mizuno Mihoko Kibe Satoko Kosaka Kazumi Natsuhara Kazuhiro Hirayama Nouhak Inthavong Sengchanh Kounnavong Shinsuke Tomita Lena Takayasu Wataru Suda Fumihiro Yagyu Masahiro Umezaki Source Type: research
