The Effect of Enzymatic and Viability Dye Treatment in Combination with Long-Range PCR on Assessing Tulane Virus Infectivity
J Virol Methods. 2024 Mar 24:114919. doi: 10.1016/j.jviromet.2024.114919. Online ahead of print.ABSTRACTHuman norovirus (HuNoV) is regularly involved in food-borne infections. To detect infectious HuNoV in food, RT-qPCR remains state of the art but also amplifies non-infectious virus. The present study combines pre-treatments, RNase and propidium monoazide, with three molecular analyses, including long-range PCR, to predominantly detect infectious Tulane virus (TuV), a culturable HuNoV surrogate. TuV was exposed to inactivating conditions to assess which molecular method most closely approximates the reduction in infectiou...
Source: Journal of Virological Methods - March 26, 2024 Category: Virology Authors: Sarah M Stoppel Bj ørn Tore Lunestad Mette Myrmel Source Type: research
The Effect of Enzymatic and Viability Dye Treatment in Combination with Long-Range PCR on Assessing Tulane Virus Infectivity
J Virol Methods. 2024 Mar 24:114919. doi: 10.1016/j.jviromet.2024.114919. Online ahead of print.ABSTRACTHuman norovirus (HuNoV) is regularly involved in food-borne infections. To detect infectious HuNoV in food, RT-qPCR remains state of the art but also amplifies non-infectious virus. The present study combines pre-treatments, RNase and propidium monoazide, with three molecular analyses, including long-range PCR, to predominantly detect infectious Tulane virus (TuV), a culturable HuNoV surrogate. TuV was exposed to inactivating conditions to assess which molecular method most closely approximates the reduction in infectiou...
Source: Journal of Virological Methods - March 26, 2024 Category: Virology Authors: Sarah M Stoppel Bj ørn Tore Lunestad Mette Myrmel Source Type: research
The Effect of Enzymatic and Viability Dye Treatment in Combination with Long-Range PCR on Assessing Tulane Virus Infectivity
J Virol Methods. 2024 Mar 24:114919. doi: 10.1016/j.jviromet.2024.114919. Online ahead of print.ABSTRACTHuman norovirus (HuNoV) is regularly involved in food-borne infections. To detect infectious HuNoV in food, RT-qPCR remains state of the art but also amplifies non-infectious virus. The present study combines pre-treatments, RNase and propidium monoazide, with three molecular analyses, including long-range PCR, to predominantly detect infectious Tulane virus (TuV), a culturable HuNoV surrogate. TuV was exposed to inactivating conditions to assess which molecular method most closely approximates the reduction in infectiou...
Source: Journal of Virological Methods - March 26, 2024 Category: Virology Authors: Sarah M Stoppel Bj ørn Tore Lunestad Mette Myrmel Source Type: research
Recovery and Quantification of Norovirus in Air Samples from Experimentally Produced Aerosols
AbstractNorovirus is the leading cause of acute gastroenteritis in humans across all age groups worldwide. Norovirus-infected patients can produce aerosolized droplets which play a role in gastroenteritis transmission. The study aimed to assess bioaerosol sampling in combination with a virus concentrating procedure to facilitate molecular detection of norovirus genogroup (G) II from experimentally contaminated aerosols. Using a nebulizer within an experimental chamber, aerosols of norovirus GII were generated at known concentrations. Air samples were then collected in both 5 mL and 20 mL water using the SKC BioSampler ...
Source: Food and Environmental Virology - March 21, 2024 Category: Virology Source Type: research
Viruses, Vol. 16, Pages 482: Wastewater-Based Epidemiology for Viral Surveillance from an Endemic Perspective: Evidence and Challenges
In conclusion, using standardized methods and an efficient monitoring strategy, WBE proves valuable for viral surveillance in pandemic and epidemic scenarios, enabling the identification of temporal–local distribution patterns that are useful for making informed public health decisions. (Source: Viruses)
Source: Viruses - March 20, 2024 Category: Virology Authors: Marco Verani Alessandra Pagani Ileana Federigi Giulia Lauretani Nebiyu Tariku Atomsa Virginia Rossi Luca Viviani Annalaura Carducci Tags: Article Source Type: research
A digital droplet PCR detection technique based on filter faster R-CNN
CONCLUSION: The ddPCR detection technique using the Filter Faster R-CNN model provides a robust detection method for ddPCR under various environmental conditions.PMID:38501420 | DOI:10.12122/j.issn.1673-4254.2024.02.17 (Source: Journal of Southern Medical University)
Source: Journal of Southern Medical University - March 19, 2024 Category: Universities & Medical Training Authors: Y Zhang B Chen J Li Y Liang H Zhang W Wu Y Zhang Source Type: research
A digital droplet PCR detection technique based on filter faster R-CNN
CONCLUSION: The ddPCR detection technique using the Filter Faster R-CNN model provides a robust detection method for ddPCR under various environmental conditions.PMID:38501420 | PMC:PMC10954537 | DOI:10.12122/j.issn.1673-4254.2024.02.17 (Source: Journal of Southern Medical University)
Source: Journal of Southern Medical University - March 19, 2024 Category: Universities & Medical Training Authors: Y Zhang B Chen J Li Y Liang H Zhang W Wu Y Zhang Source Type: research
A digital droplet PCR detection technique based on filter faster R-CNN
CONCLUSION: The ddPCR detection technique using the Filter Faster R-CNN model provides a robust detection method for ddPCR under various environmental conditions.PMID:38501420 | PMC:PMC10954537 | DOI:10.12122/j.issn.1673-4254.2024.02.17 (Source: Journal of Southern Medical University)
Source: Journal of Southern Medical University - March 19, 2024 Category: Universities & Medical Training Authors: Y Zhang B Chen J Li Y Liang H Zhang W Wu Y Zhang Source Type: research
A digital droplet PCR detection technique based on filter faster R-CNN
CONCLUSION: The ddPCR detection technique using the Filter Faster R-CNN model provides a robust detection method for ddPCR under various environmental conditions.PMID:38501420 | PMC:PMC10954537 | DOI:10.12122/j.issn.1673-4254.2024.02.17 (Source: Journal of Southern Medical University)
Source: Journal of Southern Medical University - March 19, 2024 Category: Universities & Medical Training Authors: Y Zhang B Chen J Li Y Liang H Zhang W Wu Y Zhang Source Type: research
A digital droplet PCR detection technique based on filter faster R-CNN
CONCLUSION: The ddPCR detection technique using the Filter Faster R-CNN model provides a robust detection method for ddPCR under various environmental conditions.PMID:38501420 | PMC:PMC10954537 | DOI:10.12122/j.issn.1673-4254.2024.02.17 (Source: Journal of Southern Medical University)
Source: Journal of Southern Medical University - March 19, 2024 Category: Universities & Medical Training Authors: Y Zhang B Chen J Li Y Liang H Zhang W Wu Y Zhang Source Type: research
A digital droplet PCR detection technique based on filter faster R-CNN
CONCLUSION: The ddPCR detection technique using the Filter Faster R-CNN model provides a robust detection method for ddPCR under various environmental conditions.PMID:38501420 | PMC:PMC10954537 | DOI:10.12122/j.issn.1673-4254.2024.02.17 (Source: Journal of Southern Medical University)
Source: Journal of Southern Medical University - March 19, 2024 Category: Universities & Medical Training Authors: Y Zhang B Chen J Li Y Liang H Zhang W Wu Y Zhang Source Type: research
A digital droplet PCR detection technique based on filter faster R-CNN
CONCLUSION: The ddPCR detection technique using the Filter Faster R-CNN model provides a robust detection method for ddPCR under various environmental conditions.PMID:38501420 | PMC:PMC10954537 | DOI:10.12122/j.issn.1673-4254.2024.02.17 (Source: Journal of Southern Medical University)
Source: Journal of Southern Medical University - March 19, 2024 Category: Universities & Medical Training Authors: Y Zhang B Chen J Li Y Liang H Zhang W Wu Y Zhang Source Type: research
A digital droplet PCR detection technique based on filter faster R-CNN
CONCLUSION: The ddPCR detection technique using the Filter Faster R-CNN model provides a robust detection method for ddPCR under various environmental conditions.PMID:38501420 | PMC:PMC10954537 | DOI:10.12122/j.issn.1673-4254.2024.02.17 (Source: Journal of Southern Medical University)
Source: Journal of Southern Medical University - March 19, 2024 Category: Universities & Medical Training Authors: Y Zhang B Chen J Li Y Liang H Zhang W Wu Y Zhang Source Type: research
A digital droplet PCR detection technique based on filter faster R-CNN
CONCLUSION: The ddPCR detection technique using the Filter Faster R-CNN model provides a robust detection method for ddPCR under various environmental conditions.PMID:38501420 | PMC:PMC10954537 | DOI:10.12122/j.issn.1673-4254.2024.02.17 (Source: Journal of Southern Medical University)
Source: Journal of Southern Medical University - March 19, 2024 Category: Universities & Medical Training Authors: Y Zhang B Chen J Li Y Liang H Zhang W Wu Y Zhang Source Type: research
A digital droplet PCR detection technique based on filter faster R-CNN
CONCLUSION: The ddPCR detection technique using the Filter Faster R-CNN model provides a robust detection method for ddPCR under various environmental conditions.PMID:38501420 | PMC:PMC10954537 | DOI:10.12122/j.issn.1673-4254.2024.02.17 (Source: Journal of Southern Medical University)
Source: Journal of Southern Medical University - March 19, 2024 Category: Universities & Medical Training Authors: Y Zhang B Chen J Li Y Liang H Zhang W Wu Y Zhang Source Type: research
