Examining the efficiency of porcine gastric mucin-coated magnetic beads in extraction of noroviruses from frozen berries
Food Microbiol. 2024 Jun;120:104461. doi: 10.1016/j.fm.2023.104461. Epub 2023 Dec 29.ABSTRACTHuman norovirus is the leading cause of foodborne gastroenteritis worldwide. Due to the low infectious dose of noroviruses, sensitive methodologies are required to detect and characterize small numbers of viral particles that are found in contaminated foods. The ISO 15216 method, which is internationally recognized for detection of foodborne viruses from high-risk food commodities, is based on viral precipitation, followed by RNA extraction and identification of the viral genome by RT-PCR. Although the ISO 15216 method is efficient...
Source: Food Microbiology - March 2, 2024 Category: Food Science Authors: Daniel Plante Julio Alexander Bran Barrera Maude Lord Jennifer Harlow Ir ène Iugovaz Neda Nasheri Source Type: research
An international inter-laboratory study to compare digital PCR with ISO standardized qPCR assays for the detection of norovirus GI and GII in oyster tissue
Food Microbiol. 2024 Jun;120:104478. doi: 10.1016/j.fm.2024.104478. Epub 2024 Jan 12.ABSTRACTAn optimized digital RT-PCR (RT-dPCR) assay for the detection of human norovirus GI and GII RNA was compared with ISO 15216-conform quantitative real-time RT-PCR (RT-qPCR) assays in an interlaboratory study (ILS) among eight laboratories. A duplex GI/GII RT-dPCR assay, based on the ISO 15216-oligonucleotides, was used on a Bio-Rad QX200 platform by six laboratories. Adapted assays for Qiagen Qiacuity or ThermoFisher QuantStudio 3D were used by one laboratory each. The ILS comprised quantification of norovirus RNA in the absence of ...
Source: Food Microbiology - March 2, 2024 Category: Food Science Authors: Ingeborg L A Boxman Ramia Molin Sofia Persson Anna Jur éus Claudia C C Jansen Nils P Sosef Soizick F Le Guyader Joanna Ollivier Maija Summa Maria Hautaniemi Elisabetta Suffredini Simona Di Pasquale Mette Myrmel Mamata Khatri Urska Jamnikar-Ciglenecki Dar Source Type: research
Examining the efficiency of porcine gastric mucin-coated magnetic beads in extraction of noroviruses from frozen berries
Food Microbiol. 2024 Jun;120:104461. doi: 10.1016/j.fm.2023.104461. Epub 2023 Dec 29.ABSTRACTHuman norovirus is the leading cause of foodborne gastroenteritis worldwide. Due to the low infectious dose of noroviruses, sensitive methodologies are required to detect and characterize small numbers of viral particles that are found in contaminated foods. The ISO 15216 method, which is internationally recognized for detection of foodborne viruses from high-risk food commodities, is based on viral precipitation, followed by RNA extraction and identification of the viral genome by RT-PCR. Although the ISO 15216 method is efficient...
Source: Food Microbiology - March 2, 2024 Category: Food Science Authors: Daniel Plante Julio Alexander Bran Barrera Maude Lord Jennifer Harlow Ir ène Iugovaz Neda Nasheri Source Type: research
An international inter-laboratory study to compare digital PCR with ISO standardized qPCR assays for the detection of norovirus GI and GII in oyster tissue
Food Microbiol. 2024 Jun;120:104478. doi: 10.1016/j.fm.2024.104478. Epub 2024 Jan 12.ABSTRACTAn optimized digital RT-PCR (RT-dPCR) assay for the detection of human norovirus GI and GII RNA was compared with ISO 15216-conform quantitative real-time RT-PCR (RT-qPCR) assays in an interlaboratory study (ILS) among eight laboratories. A duplex GI/GII RT-dPCR assay, based on the ISO 15216-oligonucleotides, was used on a Bio-Rad QX200 platform by six laboratories. Adapted assays for Qiagen Qiacuity or ThermoFisher QuantStudio 3D were used by one laboratory each. The ILS comprised quantification of norovirus RNA in the absence of ...
Source: Food Microbiology - March 2, 2024 Category: Food Science Authors: Ingeborg L A Boxman Ramia Molin Sofia Persson Anna Jur éus Claudia C C Jansen Nils P Sosef Soizick F Le Guyader Joanna Ollivier Maija Summa Maria Hautaniemi Elisabetta Suffredini Simona Di Pasquale Mette Myrmel Mamata Khatri Urska Jamnikar-Ciglenecki Dar Source Type: research
Examining the efficiency of porcine gastric mucin-coated magnetic beads in extraction of noroviruses from frozen berries
Food Microbiol. 2024 Jun;120:104461. doi: 10.1016/j.fm.2023.104461. Epub 2023 Dec 29.ABSTRACTHuman norovirus is the leading cause of foodborne gastroenteritis worldwide. Due to the low infectious dose of noroviruses, sensitive methodologies are required to detect and characterize small numbers of viral particles that are found in contaminated foods. The ISO 15216 method, which is internationally recognized for detection of foodborne viruses from high-risk food commodities, is based on viral precipitation, followed by RNA extraction and identification of the viral genome by RT-PCR. Although the ISO 15216 method is efficient...
Source: Food Microbiology - March 2, 2024 Category: Food Science Authors: Daniel Plante Julio Alexander Bran Barrera Maude Lord Jennifer Harlow Ir ène Iugovaz Neda Nasheri Source Type: research
An international inter-laboratory study to compare digital PCR with ISO standardized qPCR assays for the detection of norovirus GI and GII in oyster tissue
Food Microbiol. 2024 Jun;120:104478. doi: 10.1016/j.fm.2024.104478. Epub 2024 Jan 12.ABSTRACTAn optimized digital RT-PCR (RT-dPCR) assay for the detection of human norovirus GI and GII RNA was compared with ISO 15216-conform quantitative real-time RT-PCR (RT-qPCR) assays in an interlaboratory study (ILS) among eight laboratories. A duplex GI/GII RT-dPCR assay, based on the ISO 15216-oligonucleotides, was used on a Bio-Rad QX200 platform by six laboratories. Adapted assays for Qiagen Qiacuity or ThermoFisher QuantStudio 3D were used by one laboratory each. The ILS comprised quantification of norovirus RNA in the absence of ...
Source: Food Microbiology - March 2, 2024 Category: Food Science Authors: Ingeborg L A Boxman Ramia Molin Sofia Persson Anna Jur éus Claudia C C Jansen Nils P Sosef Soizick F Le Guyader Joanna Ollivier Maija Summa Maria Hautaniemi Elisabetta Suffredini Simona Di Pasquale Mette Myrmel Mamata Khatri Urska Jamnikar-Ciglenecki Dar Source Type: research
Examining the efficiency of porcine gastric mucin-coated magnetic beads in extraction of noroviruses from frozen berries
Food Microbiol. 2024 Jun;120:104461. doi: 10.1016/j.fm.2023.104461. Epub 2023 Dec 29.ABSTRACTHuman norovirus is the leading cause of foodborne gastroenteritis worldwide. Due to the low infectious dose of noroviruses, sensitive methodologies are required to detect and characterize small numbers of viral particles that are found in contaminated foods. The ISO 15216 method, which is internationally recognized for detection of foodborne viruses from high-risk food commodities, is based on viral precipitation, followed by RNA extraction and identification of the viral genome by RT-PCR. Although the ISO 15216 method is efficient...
Source: Food Microbiology - March 2, 2024 Category: Food Science Authors: Daniel Plante Julio Alexander Bran Barrera Maude Lord Jennifer Harlow Ir ène Iugovaz Neda Nasheri Source Type: research
An international inter-laboratory study to compare digital PCR with ISO standardized qPCR assays for the detection of norovirus GI and GII in oyster tissue
Food Microbiol. 2024 Jun;120:104478. doi: 10.1016/j.fm.2024.104478. Epub 2024 Jan 12.ABSTRACTAn optimized digital RT-PCR (RT-dPCR) assay for the detection of human norovirus GI and GII RNA was compared with ISO 15216-conform quantitative real-time RT-PCR (RT-qPCR) assays in an interlaboratory study (ILS) among eight laboratories. A duplex GI/GII RT-dPCR assay, based on the ISO 15216-oligonucleotides, was used on a Bio-Rad QX200 platform by six laboratories. Adapted assays for Qiagen Qiacuity or ThermoFisher QuantStudio 3D were used by one laboratory each. The ILS comprised quantification of norovirus RNA in the absence of ...
Source: Food Microbiology - March 2, 2024 Category: Food Science Authors: Ingeborg L A Boxman Ramia Molin Sofia Persson Anna Jur éus Claudia C C Jansen Nils P Sosef Soizick F Le Guyader Joanna Ollivier Maija Summa Maria Hautaniemi Elisabetta Suffredini Simona Di Pasquale Mette Myrmel Mamata Khatri Urska Jamnikar-Ciglenecki Dar Source Type: research
An international inter-laboratory study to compare digital PCR with ISO standardized qPCR assays for the detection of norovirus GI and GII in oyster tissue
Food Microbiol. 2024 Jun;120:104478. doi: 10.1016/j.fm.2024.104478. Epub 2024 Jan 12.ABSTRACTAn optimized digital RT-PCR (RT-dPCR) assay for the detection of human norovirus GI and GII RNA was compared with ISO 15216-conform quantitative real-time RT-PCR (RT-qPCR) assays in an interlaboratory study (ILS) among eight laboratories. A duplex GI/GII RT-dPCR assay, based on the ISO 15216-oligonucleotides, was used on a Bio-Rad QX200 platform by six laboratories. Adapted assays for Qiagen Qiacuity or ThermoFisher QuantStudio 3D were used by one laboratory each. The ILS comprised quantification of norovirus RNA in the absence of ...
Source: Food Microbiology - March 2, 2024 Category: Food Science Authors: Ingeborg L A Boxman Ramia Molin Sofia Persson Anna Jur éus Claudia C C Jansen Nils P Sosef Soizick F Le Guyader Joanna Ollivier Maija Summa Maria Hautaniemi Elisabetta Suffredini Simona Di Pasquale Mette Myrmel Mamata Khatri Urska Jamnikar-Ciglenecki Dar Source Type: research
Examining the efficiency of porcine gastric mucin-coated magnetic beads in extraction of noroviruses from frozen berries
Food Microbiol. 2024 Jun;120:104461. doi: 10.1016/j.fm.2023.104461. Epub 2023 Dec 29.ABSTRACTHuman norovirus is the leading cause of foodborne gastroenteritis worldwide. Due to the low infectious dose of noroviruses, sensitive methodologies are required to detect and characterize small numbers of viral particles that are found in contaminated foods. The ISO 15216 method, which is internationally recognized for detection of foodborne viruses from high-risk food commodities, is based on viral precipitation, followed by RNA extraction and identification of the viral genome by RT-PCR. Although the ISO 15216 method is efficient...
Source: Food Microbiology - March 2, 2024 Category: Food Science Authors: Daniel Plante Julio Alexander Bran Barrera Maude Lord Jennifer Harlow Ir ène Iugovaz Neda Nasheri Source Type: research
Examining the efficiency of porcine gastric mucin-coated magnetic beads in extraction of noroviruses from frozen berries
Food Microbiol. 2024 Jun;120:104461. doi: 10.1016/j.fm.2023.104461. Epub 2023 Dec 29.ABSTRACTHuman norovirus is the leading cause of foodborne gastroenteritis worldwide. Due to the low infectious dose of noroviruses, sensitive methodologies are required to detect and characterize small numbers of viral particles that are found in contaminated foods. The ISO 15216 method, which is internationally recognized for detection of foodborne viruses from high-risk food commodities, is based on viral precipitation, followed by RNA extraction and identification of the viral genome by RT-PCR. Although the ISO 15216 method is efficient...
Source: Food Microbiology - March 2, 2024 Category: Food Science Authors: Daniel Plante Julio Alexander Bran Barrera Maude Lord Jennifer Harlow Ir ène Iugovaz Neda Nasheri Source Type: research
An international inter-laboratory study to compare digital PCR with ISO standardized qPCR assays for the detection of norovirus GI and GII in oyster tissue
Food Microbiol. 2024 Jun;120:104478. doi: 10.1016/j.fm.2024.104478. Epub 2024 Jan 12.ABSTRACTAn optimized digital RT-PCR (RT-dPCR) assay for the detection of human norovirus GI and GII RNA was compared with ISO 15216-conform quantitative real-time RT-PCR (RT-qPCR) assays in an interlaboratory study (ILS) among eight laboratories. A duplex GI/GII RT-dPCR assay, based on the ISO 15216-oligonucleotides, was used on a Bio-Rad QX200 platform by six laboratories. Adapted assays for Qiagen Qiacuity or ThermoFisher QuantStudio 3D were used by one laboratory each. The ILS comprised quantification of norovirus RNA in the absence of ...
Source: Food Microbiology - March 2, 2024 Category: Food Science Authors: Ingeborg L A Boxman Ramia Molin Sofia Persson Anna Jur éus Claudia C C Jansen Nils P Sosef Soizick F Le Guyader Joanna Ollivier Maija Summa Maria Hautaniemi Elisabetta Suffredini Simona Di Pasquale Mette Myrmel Mamata Khatri Urska Jamnikar-Ciglenecki Dar Source Type: research
Examining the efficiency of porcine gastric mucin-coated magnetic beads in extraction of noroviruses from frozen berries
Food Microbiol. 2024 Jun;120:104461. doi: 10.1016/j.fm.2023.104461. Epub 2023 Dec 29.ABSTRACTHuman norovirus is the leading cause of foodborne gastroenteritis worldwide. Due to the low infectious dose of noroviruses, sensitive methodologies are required to detect and characterize small numbers of viral particles that are found in contaminated foods. The ISO 15216 method, which is internationally recognized for detection of foodborne viruses from high-risk food commodities, is based on viral precipitation, followed by RNA extraction and identification of the viral genome by RT-PCR. Although the ISO 15216 method is efficient...
Source: Food Microbiology - March 2, 2024 Category: Food Science Authors: Daniel Plante Julio Alexander Bran Barrera Maude Lord Jennifer Harlow Ir ène Iugovaz Neda Nasheri Source Type: research
An international inter-laboratory study to compare digital PCR with ISO standardized qPCR assays for the detection of norovirus GI and GII in oyster tissue
Food Microbiol. 2024 Jun;120:104478. doi: 10.1016/j.fm.2024.104478. Epub 2024 Jan 12.ABSTRACTAn optimized digital RT-PCR (RT-dPCR) assay for the detection of human norovirus GI and GII RNA was compared with ISO 15216-conform quantitative real-time RT-PCR (RT-qPCR) assays in an interlaboratory study (ILS) among eight laboratories. A duplex GI/GII RT-dPCR assay, based on the ISO 15216-oligonucleotides, was used on a Bio-Rad QX200 platform by six laboratories. Adapted assays for Qiagen Qiacuity or ThermoFisher QuantStudio 3D were used by one laboratory each. The ILS comprised quantification of norovirus RNA in the absence of ...
Source: Food Microbiology - March 2, 2024 Category: Food Science Authors: Ingeborg L A Boxman Ramia Molin Sofia Persson Anna Jur éus Claudia C C Jansen Nils P Sosef Soizick F Le Guyader Joanna Ollivier Maija Summa Maria Hautaniemi Elisabetta Suffredini Simona Di Pasquale Mette Myrmel Mamata Khatri Urska Jamnikar-Ciglenecki Dar Source Type: research
Examining the efficiency of porcine gastric mucin-coated magnetic beads in extraction of noroviruses from frozen berries
Food Microbiol. 2024 Jun;120:104461. doi: 10.1016/j.fm.2023.104461. Epub 2023 Dec 29.ABSTRACTHuman norovirus is the leading cause of foodborne gastroenteritis worldwide. Due to the low infectious dose of noroviruses, sensitive methodologies are required to detect and characterize small numbers of viral particles that are found in contaminated foods. The ISO 15216 method, which is internationally recognized for detection of foodborne viruses from high-risk food commodities, is based on viral precipitation, followed by RNA extraction and identification of the viral genome by RT-PCR. Although the ISO 15216 method is efficient...
Source: Food Microbiology - March 2, 2024 Category: Food Science Authors: Daniel Plante Julio Alexander Bran Barrera Maude Lord Jennifer Harlow Ir ène Iugovaz Neda Nasheri Source Type: research
