Examining the efficiency of porcine gastric mucin-coated magnetic beads in extraction of noroviruses from frozen berries
Food Microbiol. 2024 Jun;120:104461. doi: 10.1016/j.fm.2023.104461. Epub 2023 Dec 29.ABSTRACTHuman norovirus is the leading cause of foodborne gastroenteritis worldwide. Due to the low infectious dose of noroviruses, sensitive methodologies are required to detect and characterize small numbers of viral particles that are found in contaminated foods. The ISO 15216 method, which is internationally recognized for detection of foodborne viruses from high-risk food commodities, is based on viral precipitation, followed by RNA extraction and identification of the viral genome by RT-PCR. Although the ISO 15216 method is efficient...
Source: Food Microbiology - March 2, 2024 Category: Food Science Authors: Daniel Plante Julio Alexander Bran Barrera Maude Lord Jennifer Harlow Ir ène Iugovaz Neda Nasheri Source Type: research
An international inter-laboratory study to compare digital PCR with ISO standardized qPCR assays for the detection of norovirus GI and GII in oyster tissue
Food Microbiol. 2024 Jun;120:104478. doi: 10.1016/j.fm.2024.104478. Epub 2024 Jan 12.ABSTRACTAn optimized digital RT-PCR (RT-dPCR) assay for the detection of human norovirus GI and GII RNA was compared with ISO 15216-conform quantitative real-time RT-PCR (RT-qPCR) assays in an interlaboratory study (ILS) among eight laboratories. A duplex GI/GII RT-dPCR assay, based on the ISO 15216-oligonucleotides, was used on a Bio-Rad QX200 platform by six laboratories. Adapted assays for Qiagen Qiacuity or ThermoFisher QuantStudio 3D were used by one laboratory each. The ILS comprised quantification of norovirus RNA in the absence of ...
Source: Food Microbiology - March 2, 2024 Category: Food Science Authors: Ingeborg L A Boxman Ramia Molin Sofia Persson Anna Jur éus Claudia C C Jansen Nils P Sosef Soizick F Le Guyader Joanna Ollivier Maija Summa Maria Hautaniemi Elisabetta Suffredini Simona Di Pasquale Mette Myrmel Mamata Khatri Urska Jamnikar-Ciglenecki Dar Source Type: research
An international inter-laboratory study to compare digital PCR with ISO standardized qPCR assays for the detection of norovirus GI and GII in oyster tissue
Food Microbiol. 2024 Jun;120:104478. doi: 10.1016/j.fm.2024.104478. Epub 2024 Jan 12.ABSTRACTAn optimized digital RT-PCR (RT-dPCR) assay for the detection of human norovirus GI and GII RNA was compared with ISO 15216-conform quantitative real-time RT-PCR (RT-qPCR) assays in an interlaboratory study (ILS) among eight laboratories. A duplex GI/GII RT-dPCR assay, based on the ISO 15216-oligonucleotides, was used on a Bio-Rad QX200 platform by six laboratories. Adapted assays for Qiagen Qiacuity or ThermoFisher QuantStudio 3D were used by one laboratory each. The ILS comprised quantification of norovirus RNA in the absence of ...
Source: Food Microbiology - March 2, 2024 Category: Food Science Authors: Ingeborg L A Boxman Ramia Molin Sofia Persson Anna Jur éus Claudia C C Jansen Nils P Sosef Soizick F Le Guyader Joanna Ollivier Maija Summa Maria Hautaniemi Elisabetta Suffredini Simona Di Pasquale Mette Myrmel Mamata Khatri Urska Jamnikar-Ciglenecki Dar Source Type: research
Attitudes toward a future norovirus vaccine among members of an integrated healthcare delivery system in Portland, Oregon, 2016-2017
Volume 20, Issue 1, December 2024 . (Source: Human Vaccines and Immunotherapeutics)
Source: Human Vaccines and Immunotherapeutics - February 28, 2024 Category: Allergy & Immunology Authors: Holly C. GroomMark SchmidtLaura E. CalderwoodSara A. MirzaClaire MattisonSuzanne SalasJudy DonaldAllison L. Nalewaya Science Programs Department, Kaiser Permanente Center for Health Research, Portland, OR, USAb National Center for Immunization and Respira Source Type: research
Detection and Characterization of Bacterial and Viral Acute Gastroenteritis among Outpatient Children under 5 Years Old in Guangzhou, China
This study underscores the burden posed by coinfections and highlights the clinical characteristics associated with AGE when accompanied by coinfections among children under 5 years old.PMID:38412529 | DOI:10.4269/ajtmh.23-0725 (Source: The American Journal of Tropical Medicine and Hygiene)
Source: The American Journal of Tropical Medicine and Hygiene - February 27, 2024 Category: Tropical Medicine Authors: Xin Luo Jiankai Deng Mingyong Luo Nan Yu Xiaoyan Che Source Type: research
Detection and Characterization of Bacterial and Viral Acute Gastroenteritis among Outpatient Children under 5 Years Old in Guangzhou, China
This study underscores the burden posed by coinfections and highlights the clinical characteristics associated with AGE when accompanied by coinfections among children under 5 years old.PMID:38412529 | DOI:10.4269/ajtmh.23-0725 (Source: Am J Trop Med Hyg)
Source: Am J Trop Med Hyg - February 27, 2024 Category: Infectious Diseases Authors: Xin Luo Jiankai Deng Mingyong Luo Nan Yu Xiaoyan Che Source Type: research
Detection and Characterization of Bacterial and Viral Acute Gastroenteritis among Outpatient Children under 5 Years Old in Guangzhou, China
This study underscores the burden posed by coinfections and highlights the clinical characteristics associated with AGE when accompanied by coinfections among children under 5 years old.PMID:38412529 | DOI:10.4269/ajtmh.23-0725 (Source: The American Journal of Tropical Medicine and Hygiene)
Source: The American Journal of Tropical Medicine and Hygiene - February 27, 2024 Category: Tropical Medicine Authors: Xin Luo Jiankai Deng Mingyong Luo Nan Yu Xiaoyan Che Source Type: research
Detection and Characterization of Bacterial and Viral Acute Gastroenteritis among Outpatient Children under 5 Years Old in Guangzhou, China
This study underscores the burden posed by coinfections and highlights the clinical characteristics associated with AGE when accompanied by coinfections among children under 5 years old.PMID:38412529 | DOI:10.4269/ajtmh.23-0725 (Source: Am J Trop Med Hyg)
Source: Am J Trop Med Hyg - February 27, 2024 Category: Infectious Diseases Authors: Xin Luo Jiankai Deng Mingyong Luo Nan Yu Xiaoyan Che Source Type: research
Detection and Characterization of Bacterial and Viral Acute Gastroenteritis among Outpatient Children under 5 Years Old in Guangzhou, China
This study underscores the burden posed by coinfections and highlights the clinical characteristics associated with AGE when accompanied by coinfections among children under 5 years old.PMID:38412529 | DOI:10.4269/ajtmh.23-0725 (Source: The American Journal of Tropical Medicine and Hygiene)
Source: The American Journal of Tropical Medicine and Hygiene - February 27, 2024 Category: Tropical Medicine Authors: Xin Luo Jiankai Deng Mingyong Luo Nan Yu Xiaoyan Che Source Type: research
Hydroponic Nutrient Solution Temperature Impacts Tulane Virus Persistence over Time
This study aids in the (i) identification of possible food safety risks associated with hydroponic systems specifically related to nutrient solution temperature and (ii) generation of data to perform risk asses sments within CEA leaf lettuce operations to inform risk management strategies for the reduction of foodborne outbreaks, fresh produce recalls, and economic losses. (Source: Food and Environmental Virology)
Source: Food and Environmental Virology - February 27, 2024 Category: Virology Source Type: research
Characterization of cleavage patterns and assembly of N-terminally modified GII.6 norovirus VP1 proteins
AbstractWhen expressedin vitro, the major capsid protein VP1 of a norovirus (NoV) can self-assemble into virus-like particles (VLPs), and its N-terminus can tolerate foreign sequences without the assembly being affected. We explored the effects of adding an N-terminal sequence to the VP1 of a GII.6 NoV strain on its cleavage and assembly. Sequences of varying lengths derived from the minor capsid protein VP2 were added to the VP1 N-terminus. Using a recombinant baculovirus expression system, the fusion proteins were expressed, and their cleavage patterns and assembly were analyzed using mass spectrometry and transmission e...
Source: Archives of Virology - February 22, 2024 Category: Virology Source Type: research
Viruses, Vol. 16, Pages 317: Assessment of Gastroenteric Viruses in Marketed Bivalve Mollusks in the Tourist Cities of Rio de Janeiro, Brazil, 2022
This study investigated the prevalence and genetic diversity of gastroenteric viruses in mussels and oysters in Rio de Janeiro, Brazil. One hundred and thirty-four marketed bivalve samples were obtained between January and December 2022. The viral analysis was performed according to ISO/TS 15216, and the screening revealed the detection of norovirus GII/GI (40.3%), sapovirus (SaV; 12.7%), human mastadenovirus (7.5%), and rotavirus A (RVA; 5.9%). In total, 44.8% (60) of shellfish samples tested positive for one or more viruses, 46.7% (28/60) of the positive samples tested positive for a single viral agent, 26.7% (16) tested...
Source: Viruses - February 20, 2024 Category: Virology Authors: Carina Pacheco Cantelli Guilherme Caetano Lanzieri Tavares Sylvia Kahwage Sarmento Fernanda Marcicano Burlandy Tulio Machado Fumian Adriana Gon çalves Maranhão Emanuelle de Souza Ramalho Ferreira da Silva Marco Aur élio Pereira Horta Marize Pereira Mia Tags: Article Source Type: research
A systematic overview of metal nanoparticles as alternative disinfectants for emerging SARS-CoV-2 variants
Arch Microbiol. 2024 Feb 19;206(3):111. doi: 10.1007/s00203-023-03818-z.ABSTRACTCoronaviruses are a diverse family of viruses, and new strains can emerge. While the majority of coronavirus strains cause mild respiratory illnesses, a few are responsible for severe diseases such as Severe Acute Respiratory Syndrome (SARS) and Middle East Respiratory Syndrome (MERS). SARS-CoV-2, the virus responsible for COVID-19, is an example of a coronavirus that has led to a pandemic. Coronaviruses can mutate over time, potentially leading to the emergence of new variants. Some of these variants may have increased transmissibility or resi...
Source: Archives of Microbiology - February 19, 2024 Category: Microbiology Authors: Sanjeev Kumar Soni Tripta Marya Apurav Sharma Bishakha Thakur Raman Soni Source Type: research
Molnupiravir inhibits human norovirus and rotavirus replication in 3D human intestinal enteroids
Antiviral Res. 2024 Feb 17:105839. doi: 10.1016/j.antiviral.2024.105839. Online ahead of print.ABSTRACTHuman norovirus (HuNoV) and human rotavirus (HRV) are the leading causes of gastrointestinal diarrhea. There are no approved antivirals and rotavirus vaccines are insufficient to cease HRV associated mortality. Furthermore, treatment of chronically infected immunocompromised patients is limited to off-label compassionate use of repurposed antivirals with limited efficacy, highlighting the urgent need of potent and specific antivirals for HuNoV and HRV. Recently, a major breakthrough in the in vitro cultivation of HuNoV an...
Source: Antiviral Research - February 19, 2024 Category: Virology Authors: Nanci Santos-Ferreira Jana Van Dycke Winston Chiu Johan Neyts Jelle Matthijnssens Joana Rocha-Pereira Source Type: research
Molnupiravir inhibits human norovirus and rotavirus replication in 3D human intestinal enteroids
Antiviral Res. 2024 Feb 17:105839. doi: 10.1016/j.antiviral.2024.105839. Online ahead of print.ABSTRACTHuman norovirus (HuNoV) and human rotavirus (HRV) are the leading causes of gastrointestinal diarrhea. There are no approved antivirals and rotavirus vaccines are insufficient to cease HRV associated mortality. Furthermore, treatment of chronically infected immunocompromised patients is limited to off-label compassionate use of repurposed antivirals with limited efficacy, highlighting the urgent need of potent and specific antivirals for HuNoV and HRV. Recently, a major breakthrough in the in vitro cultivation of HuNoV an...
Source: Antiviral Research - February 19, 2024 Category: Virology Authors: Nanci Santos-Ferreira Jana Van Dycke Winston Chiu Johan Neyts Jelle Matthijnssens Joana Rocha-Pereira Source Type: research
