A DNA vaccine against GII.4 human norovirus VP1 induces blocking antibody production and T cell responses

In this study, we constructed a CMV/R vector expressing a major structural protein, VP1, of GII.4 HuNoV (CMV/R-GII.4 HuNoV VP1). Transfection of CMV/R-GII.4 HuNoV VP1 into human embryonic kidney 293T (HEK293T) cells resulted in successful expression of VP1 proteins in vitro. Intramuscular or intradermal immunization of mice with the CMV/R-GII.4 HuNoV VP1 construct elicited the production of blocking antibodies and activation of T cell responses against GII.4 HuNoV VP1. Our collective data support the utility of CMV/R-GII.4 HuNoV VP1 as a promising DNA vaccine candidate against GII.4 HuNoV.PMID:38320930 | DOI:10.1016/j.vaccine.2024.01.090

Source: Vaccine - February 6, 2024 Category: Allergy & Immunology Authors: Na-Eun Kim Mun-Jin Kim Bum Ju Park Jung Won Kwon Jae Myun Lee Jung-Hwan Park Yoon-Jae Song Source Type: research