Structural Analysis of Protein Complexes by Cryo-Electron Microscopy
Methods Mol Biol. 2024;2715:431-470. doi: 10.1007/978-1-0716-3445-5_27.ABSTRACTStructural studies of bio-complexes using single particle cryo-Electron Microscopy (cryo-EM) is nowadays a well-established technique in structural biology and has become competitive with X-ray crystallography. Development of digital registration systems for electron microscopy images and algorithms for the fast and efficient processing of the recorded images and their following analysis has facilitated the determination of structures at near-atomic resolution. The latest advances in EM have enabled the determination of protein complex structure...
Source: Mol Biol Cell - November 6, 2023 Category: Molecular Biology Authors: Athanasios Ignatiou K évin Macé Adam Redzej Tiago R D Costa Gabriel Waksman Elena V Orlova Source Type: research
Structural Analysis of Protein Complexes by Cryo-Electron Microscopy
Methods Mol Biol. 2024;2715:431-470. doi: 10.1007/978-1-0716-3445-5_27.ABSTRACTStructural studies of bio-complexes using single particle cryo-Electron Microscopy (cryo-EM) is nowadays a well-established technique in structural biology and has become competitive with X-ray crystallography. Development of digital registration systems for electron microscopy images and algorithms for the fast and efficient processing of the recorded images and their following analysis has facilitated the determination of structures at near-atomic resolution. The latest advances in EM have enabled the determination of protein complex structure...
Source: Cell Research - November 6, 2023 Category: Cytology Authors: Athanasios Ignatiou K évin Macé Adam Redzej Tiago R D Costa Gabriel Waksman Elena V Orlova Source Type: research
Structural Analysis of Protein Complexes by Cryo-Electron Microscopy
Methods Mol Biol. 2024;2715:431-470. doi: 10.1007/978-1-0716-3445-5_27.ABSTRACTStructural studies of bio-complexes using single particle cryo-Electron Microscopy (cryo-EM) is nowadays a well-established technique in structural biology and has become competitive with X-ray crystallography. Development of digital registration systems for electron microscopy images and algorithms for the fast and efficient processing of the recorded images and their following analysis has facilitated the determination of structures at near-atomic resolution. The latest advances in EM have enabled the determination of protein complex structure...
Source: Mol Biol Cell - November 6, 2023 Category: Molecular Biology Authors: Athanasios Ignatiou K évin Macé Adam Redzej Tiago R D Costa Gabriel Waksman Elena V Orlova Source Type: research
175 Basic life support training with augmented reality with smart glasses vs. traditional face-to-face education. A quasi-experimental pilot study
(Source: Resuscitation)
Source: Resuscitation - October 31, 2023 Category: Emergency Medicine Authors: Silvia Aranda-Garc ía, Martín Otero-Agra, Carlos Berlanga-Macías, Felipe Fernández-Méndez, Roberto Barcala-Furelos, Júlia Domingo, Adriana Seijas-Vijande, Santiago Martínez-Isasi, Antonio Rodriguez-Nunez Tags: Poster presentation Source Type: research
Sensors, Vol. 23, Pages 8830: Damage Identification of Railway Bridges through Temporal Autoregressive Modeling
r Ivorra
The damage identification of railway bridges poses a formidable challenge given the large variability in the environmental and operational conditions that such structures are subjected to along their lifespan. To address this challenge, this paper proposes a novel damage identification approach exploiting continuously extracted time series of autoregressive (AR) coefficients from strain data with moving train loads as highly sensitive damage features. Through a statistical pattern recognition algorithm involving data clustering and quality control charts, the proposed approach offers a set of sensor-level dama...
Source: Sensors - October 30, 2023 Category: Biotechnology Authors: Stefano Anastasia Enrique Garc ía-Macías Filippo Ubertini Vincenzo Gattulli Salvador Ivorra Tags: Article Source Type: research
Molecules, Vol. 28, Pages 7283: The Synthesis of Novel aza-Steroids and & alpha;, & beta;-Unsaturated-Cyanoketone from Diosgenin
Molecules, Vol. 28, Pages 7283: The Synthesis of Novel aza-Steroids and α, β-Unsaturated-Cyanoketone from Diosgenin
Molecules doi: 10.3390/molecules28217283
Authors:
Dayana Mesa
Yarelys E. Augusto
Giselle Hernández
Juan P. Figueroa-Macías
Francisco Coll
Andrés F. Olea
María Núñez
Hernán Astudillo Campo
Yamilet Coll
Luis Espinoza
Recent studies have demonstrated the antiproliferative and cytotoxic effects of aza-steroids and steroidal sapogenins on human cancer cell lines. The scientific community has shown a growing interest in these compounds as drug candidates for cance...
Source: Molecules - October 26, 2023 Category: Chemistry Authors: Dayana Mesa Yarelys E. Augusto Giselle Hern ández Juan P. Figueroa-Mac ías Francisco Coll Andr és F. Olea Mar ía Núñez Hern án Astudillo Campo Yamilet Coll Luis Espinoza Tags: Article Source Type: research
The novel FAAH inhibitor, MCH1, reduces the infarction area in the motor cortex-related region but does not affect the sensorimotor function or memory and spatial learning in rats exposed to transient middle cerebral artery occlusion
Brain Res. 2023 Oct 19:148636. doi: 10.1016/j.brainres.2023.148636. Online ahead of print.ABSTRACTMacamides, amides of fatty acids first isolated from maca (Lepidium meyenii) are potentially responsible for the reduction of ischemic injury in the stroke animal model followed by maca extract administration. This deduction comes from its ability to inhibit the fatty acid amide hydrolase activity, an enzyme related to the endocannabinoid anandamide hydrolysis. However, no study about the effects of isolated macamides on in-vivo models has been published yet. Our objective was to evaluate the effect of a 10-day 30 mg/kg i.p. M...
Source: Brain Research - October 21, 2023 Category: Neurology Authors: K Taboada-Rosell F A Castro-Garc ía C Medina-Sald ívar S R Cruz-Visalaya L F Pacheco-Otalora Source Type: research
Bixa orellana ethyl acetate fraction and its isolated compound ellagic acid attenuate the progression of MIA-induced osteoarthritis in rat knees
Biomed Pharmacother. 2023 Oct 13;168:115644. doi: 10.1016/j.biopha.2023.115644. Online ahead of print.ABSTRACTOsteoarthritis (OA) is a pathology that is characterized by progressive erosion of articular cartilage. In this context, medicinal plants have become relevant tools regarding their potential role in the prevention and treatment of OA, being safe and effective. The aim of this work was investigate the therapeutic efficacy of the ethyl acetate fraction of Bixa orellana leaves (BoEA) and ellagic acid (ElAc) for the therapeutic treatment of OA induced by monosodium iodoacetate (MIA) in rats. The plant material was extr...
Source: Biomedicine and pharmacotherapy = Biomedecine and pharmacotherapie - October 15, 2023 Category: Drugs & Pharmacology Authors: Luis Ângelo Macêdo Santiago Ana Caroline Santos Ata íde Danilo de Ara újo Morais Aldilene da Silva Lima Nath álya Dos Santos Martins Adriana Vivian Costa Ara újo Dourado Rachel Melo Ribeiro L ídio Gonçalves Lima-Neto Joicy Cortez de S á Sousa Cl Source Type: research
Bixa orellana ethyl acetate fraction and its isolated compound ellagic acid attenuate the progression of MIA-induced osteoarthritis in rat knees
Biomed Pharmacother. 2023 Oct 13;168:115644. doi: 10.1016/j.biopha.2023.115644. Online ahead of print.ABSTRACTOsteoarthritis (OA) is a pathology that is characterized by progressive erosion of articular cartilage. In this context, medicinal plants have become relevant tools regarding their potential role in the prevention and treatment of OA, being safe and effective. The aim of this work was investigate the therapeutic efficacy of the ethyl acetate fraction of Bixa orellana leaves (BoEA) and ellagic acid (ElAc) for the therapeutic treatment of OA induced by monosodium iodoacetate (MIA) in rats. The plant material was extr...
Source: Biomedicine and pharmacotherapy = Biomedecine and pharmacotherapie - October 15, 2023 Category: Drugs & Pharmacology Authors: Luis Ângelo Macêdo Santiago Ana Caroline Santos Ata íde Danilo de Ara újo Morais Aldilene da Silva Lima Nath álya Dos Santos Martins Adriana Vivian Costa Ara újo Dourado Rachel Melo Ribeiro L ídio Gonçalves Lima-Neto Joicy Cortez de S á Sousa Cl Source Type: research
T1 mapping of myocardium in rats using self-gated golden-angle acquisition
CONCLUSIONS: A new 2D and 3D method for quantification of myocardial T1 in rats was proposed. We have shown the capability of both techniques to distinguish between normal and remodeling myocardial tissue. We have shown the effects of image-reconstruction regularization weights and acquisition length on the T1 estimates.PMID:37811699 | DOI:10.1002/mrm.29846 (Source: Magnetic Resonance in Medicine)
Source: Magnetic Resonance in Medicine - October 9, 2023 Category: Radiology Authors: Ji ří Vitouš Radovan Ji řík Tibor Stra čina Michal Hendrych Jaroslav N ádeníček Ond řej Macíček Ye Tian Lucie Kr átká Eva Dra žanová Marie Nov áková Petr Babula Roman Panovsk ý Edward DiBella Zenon Star čuk Source Type: research
Exploring oxidative stress pathways in Geobacter sulfurreducens: the redox network between MacA peroxidase and triheme periplasmic cytochromes
The recent reclassification of the strict anaerobe Geobacter sulfurreducens bacterium as aerotolerant brought attention for oxidative stress protection pathways. Although the electron transfer pathways for oxygen detoxification are not well established, evidence was obtained for the formation of a redox complex between the periplasmic triheme cytochrome PpcA and the diheme cytochrome peroxidase MacA. In the latter, the reduction of the high-potential heme triggers a conformational change that displaces the axial histidine of the low-potential heme with peroxidase activity. More recently, a possible involvement of the trihe...
Source: Frontiers in Microbiology - October 4, 2023 Category: Microbiology Source Type: research
Symmetric 4,6-Dialkyl/arylamino-5-nitropyrimidines: Theoretical Explanation of Why Aminolysis of Alkoxy Groups Is Favoured over Chlorine Aminolysis in Nitro-Activated Pyrimidines.
This article is licensed under aCreative Commons Attribution-NonCommercial 3.0 Unported Licence.Laura Cordoba Gomez, Álvaro Lorente-Macías, Maria Jose Pineda de las Infantas, Andres Garzon, Juan Diaz-Mochon A new synthetic route to obtain symmetric disubstituted alkyl/arylaminopyrimidines under mild conditions is presented, which can be used to generate new purine libraries for drug discovery. We investigated the unexpected... The content of this RSS Feed (c) The Royal Society of Chemistry (Source: RSC - New J. Chem. latest articles)
Source: RSC - New J. Chem. latest articles - October 2, 2023 Category: Chemistry Authors: Laura Cordoba Gomez Source Type: research
VP226 Post-hoc analysis of compound motor action potential from clinical trials of intravenous onasemnogene abeparvovec for spinal muscular atrophy
Spinal muscular atrophy (SMA) is characterized by muscle weakness, and, therefore, the progression of the disease and the potential efficacy of therapies can be evaluated by means of electrophysiologic recordings. The compound motor action potential (CMAP) summarizes muscle fiber action potentials, and it correlates with the degree of motor nerve innervation, making it potentially valuable as an SMA biomarker. CMAP was evaluated in three completed clinical trials of intravenous onasemnogene abeparvovec for patients with SMA type 1 and two copies of the survival motor neuron 2 gene (SMN2): START (NCT02122952), STR1VE (NCT03...
Source: Neuromuscular Disorders - October 1, 2023 Category: Neurology Authors: B. McGill, J. Maca, S. Tauscher-Wisniewski, T. Macek Source Type: research
Harder, better, faster, stronger? Retrospective chart review of adverse events of interactions between adaptogens and antidepressant drugs
Conclusion: Clinicians should monitor the adverse events associated with the concomitant use of adaptogens and antidepressant drugs in patients with mental disorders. Aggregation of side effects and pharmacokinetic interactions (inhibition of CYP and p-glycoprotein) between those medicines may result in clinically significant adverse events. (Source: Frontiers in Pharmacology)
Source: Frontiers in Pharmacology - September 27, 2023 Category: Drugs & Pharmacology Source Type: research
Genes, Vol. 14, Pages 1775: Association of PCSK1 and PPARG1 Allelic Variants with Obesity and Metabolic Syndrome in Mexican Adults
Conclusions: The associations found in this study, mainly between allelic variants of PCSK1 and metabolic traits, obesity, and metabolic syndrome, may represent a risk for developing metabolic diseases in adult subjects from northwestern Mexico. (Source: Genes)
Source: Genes - September 8, 2023 Category: Genetics & Stem Cells Authors: Jorge Velazquez-Roman Uriel A. Angulo-Zamudio Nidia Leon-Sicairos Hector Flores-Villase ñor Miriam Benitez-Baez Ana Espinoza-Salom ón Alejandra Karam-Le ón Hugo Villamil-Ram írez Samuel Canizales-Quinteros Luis Mac ías-Kauffer Jose Monroy-Higuera Eri Tags: Article Source Type: research
