Cross-regulation of Listeria monocytogenes and the host ubiquitin system in listeriosis
Eur J Cell Biol. 2024 Feb 28;103(2):151401. doi: 10.1016/j.ejcb.2024.151401. Online ahead of print.ABSTRACTThe facultative intracellular bacterium Listeria (L.) monocytogenes may cause severe diseases in humans and animals. The control of listeriosis/L. monocytogenes requires the concerted action of cells of the innate and adaptive immune systems. In this regard, cell-intrinsic immunity of infected cells, activated by the immune responses, is crucial for the control and elimination intracellular L. monocytogenes. Both the immune response against L. monocytogenes and cell intrinsic pathogen control are critically regulated ...
Source: European Journal of Cell Biology - March 5, 2024 Category: Cytology Authors: Yuan Zhuang Johanna B Fischer Gopala Nishanth Dirk Schl üter Source Type: research
Immortalised murine R349P desmin knock-in myotubes exhibit a reduced proton leak and decreased ADP/ATP translocase levels in purified mitochondria
Eur J Cell Biol. 2024 Feb 20;103(2):151399. doi: 10.1016/j.ejcb.2024.151399. Online ahead of print.ABSTRACTDesmin gene mutations cause myopathies and cardiomyopathies. Our previously characterised R349P desminopathy mice, which carry the ortholog of the common human desmin mutation R350P, showed marked alterations in mitochondrial morphology and function in muscle tissue. By isolating skeletal muscle myoblasts from offspring of R349P desminopathy and p53 knock-out mice, we established an immortalised cellular disease model. Heterozygous and homozygous R349P desmin knock-in and wild-type myoblasts could be well differentiat...
Source: European Journal of Cell Biology - February 27, 2024 Category: Cytology Authors: Carolin Berwanger Dominic Terres Dominik Pesta Britta Eggers Katrin Marcus Ilka Wittig Rudolf J Wiesner Rolf Schr öder Christoph S Clemen Source Type: research
Immortalised murine R349P desmin knock-in myotubes exhibit a reduced proton leak and decreased ADP/ATP translocase levels in purified mitochondria
Eur J Cell Biol. 2024 Feb 20;103(2):151399. doi: 10.1016/j.ejcb.2024.151399. Online ahead of print.ABSTRACTDesmin gene mutations cause myopathies and cardiomyopathies. Our previously characterised R349P desminopathy mice, which carry the ortholog of the common human desmin mutation R350P, showed marked alterations in mitochondrial morphology and function in muscle tissue. By isolating skeletal muscle myoblasts from offspring of R349P desminopathy and p53 knock-out mice, we established an immortalised cellular disease model. Heterozygous and homozygous R349P desmin knock-in and wild-type myoblasts could be well differentiat...
Source: European Journal of Cell Biology - February 27, 2024 Category: Cytology Authors: Carolin Berwanger Dominic Terres Dominik Pesta Britta Eggers Katrin Marcus Ilka Wittig Rudolf J Wiesner Rolf Schr öder Christoph S Clemen Source Type: research
Immortalised murine R349P desmin knock-in myotubes exhibit a reduced proton leak and decreased ADP/ATP translocase levels in purified mitochondria
Eur J Cell Biol. 2024 Feb 20;103(2):151399. doi: 10.1016/j.ejcb.2024.151399. Online ahead of print.ABSTRACTDesmin gene mutations cause myopathies and cardiomyopathies. Our previously characterised R349P desminopathy mice, which carry the ortholog of the common human desmin mutation R350P, showed marked alterations in mitochondrial morphology and function in muscle tissue. By isolating skeletal muscle myoblasts from offspring of R349P desminopathy and p53 knock-out mice, we established an immortalised cellular disease model. Heterozygous and homozygous R349P desmin knock-in and wild-type myoblasts could be well differentiat...
Source: European Journal of Cell Biology - February 27, 2024 Category: Cytology Authors: Carolin Berwanger Dominic Terres Dominik Pesta Britta Eggers Katrin Marcus Ilka Wittig Rudolf J Wiesner Rolf Schr öder Christoph S Clemen Source Type: research
Immortalised murine R349P desmin knock-in myotubes exhibit a reduced proton leak and decreased ADP/ATP translocase levels in purified mitochondria
Eur J Cell Biol. 2024 Feb 20;103(2):151399. doi: 10.1016/j.ejcb.2024.151399. Online ahead of print.ABSTRACTDesmin gene mutations cause myopathies and cardiomyopathies. Our previously characterised R349P desminopathy mice, which carry the ortholog of the common human desmin mutation R350P, showed marked alterations in mitochondrial morphology and function in muscle tissue. By isolating skeletal muscle myoblasts from offspring of R349P desminopathy and p53 knock-out mice, we established an immortalised cellular disease model. Heterozygous and homozygous R349P desmin knock-in and wild-type myoblasts could be well differentiat...
Source: European Journal of Cell Biology - February 27, 2024 Category: Cytology Authors: Carolin Berwanger Dominic Terres Dominik Pesta Britta Eggers Katrin Marcus Ilka Wittig Rudolf J Wiesner Rolf Schr öder Christoph S Clemen Source Type: research
Immortalised murine R349P desmin knock-in myotubes exhibit a reduced proton leak and decreased ADP/ATP translocase levels in purified mitochondria
Eur J Cell Biol. 2024 Feb 20;103(2):151399. doi: 10.1016/j.ejcb.2024.151399. Online ahead of print.ABSTRACTDesmin gene mutations cause myopathies and cardiomyopathies. Our previously characterised R349P desminopathy mice, which carry the ortholog of the common human desmin mutation R350P, showed marked alterations in mitochondrial morphology and function in muscle tissue. By isolating skeletal muscle myoblasts from offspring of R349P desminopathy and p53 knock-out mice, we established an immortalised cellular disease model. Heterozygous and homozygous R349P desmin knock-in and wild-type myoblasts could be well differentiat...
Source: European Journal of Cell Biology - February 27, 2024 Category: Cytology Authors: Carolin Berwanger Dominic Terres Dominik Pesta Britta Eggers Katrin Marcus Ilka Wittig Rudolf J Wiesner Rolf Schr öder Christoph S Clemen Source Type: research
NANOS1 restricts oral cancer cell motility and TGF- ß signaling
Eur J Cell Biol. 2024 Feb 21;103(2):151400. doi: 10.1016/j.ejcb.2024.151400. Online ahead of print.ABSTRACTOral squamous cell carcinoma (OSCC) is the most frequent type of cancer of the head and neck area accounting for approx. 377,000 new cancer cases every year. The epithelial-to-mesenchymal transition (EMT) program plays an important role in OSCC progression and metastasis therefore contributing to a poor prognosis in patients with advanced disease. Transforming growth factor beta (TGF-ß) is a powerful inducer of EMT thereby increasing cancer cell aggressiveness. Here, we aimed at identifying RNA-binding proteins (RBPs...
Source: European Journal of Cell Biology - February 24, 2024 Category: Cytology Authors: Julia Rosemann Jonas Pyko Roland Jacob Jana Macho Matthias Kappler Alexander W Eckert Monika Haemmerle Tony Gutschner Source Type: research
NANOS1 restricts oral cancer cell motility and TGF- ß signaling
Eur J Cell Biol. 2024 Feb 21;103(2):151400. doi: 10.1016/j.ejcb.2024.151400. Online ahead of print.ABSTRACTOral squamous cell carcinoma (OSCC) is the most frequent type of cancer of the head and neck area accounting for approx. 377,000 new cancer cases every year. The epithelial-to-mesenchymal transition (EMT) program plays an important role in OSCC progression and metastasis therefore contributing to a poor prognosis in patients with advanced disease. Transforming growth factor beta (TGF-ß) is a powerful inducer of EMT thereby increasing cancer cell aggressiveness. Here, we aimed at identifying RNA-binding proteins (RBPs...
Source: European Journal of Cell Biology - February 24, 2024 Category: Cytology Authors: Julia Rosemann Jonas Pyko Roland Jacob Jana Macho Matthias Kappler Alexander W Eckert Monika Haemmerle Tony Gutschner Source Type: research
NANOS1 restricts oral cancer cell motility and TGF- ß signaling
Eur J Cell Biol. 2024 Feb 21;103(2):151400. doi: 10.1016/j.ejcb.2024.151400. Online ahead of print.ABSTRACTOral squamous cell carcinoma (OSCC) is the most frequent type of cancer of the head and neck area accounting for approx. 377,000 new cancer cases every year. The epithelial-to-mesenchymal transition (EMT) program plays an important role in OSCC progression and metastasis therefore contributing to a poor prognosis in patients with advanced disease. Transforming growth factor beta (TGF-ß) is a powerful inducer of EMT thereby increasing cancer cell aggressiveness. Here, we aimed at identifying RNA-binding proteins (RBPs...
Source: European Journal of Cell Biology - February 24, 2024 Category: Cytology Authors: Julia Rosemann Jonas Pyko Roland Jacob Jana Macho Matthias Kappler Alexander W Eckert Monika Haemmerle Tony Gutschner Source Type: research
NANOS1 restricts oral cancer cell motility and TGF- ß signaling
Eur J Cell Biol. 2024 Feb 21;103(2):151400. doi: 10.1016/j.ejcb.2024.151400. Online ahead of print.ABSTRACTOral squamous cell carcinoma (OSCC) is the most frequent type of cancer of the head and neck area accounting for approx. 377,000 new cancer cases every year. The epithelial-to-mesenchymal transition (EMT) program plays an important role in OSCC progression and metastasis therefore contributing to a poor prognosis in patients with advanced disease. Transforming growth factor beta (TGF-ß) is a powerful inducer of EMT thereby increasing cancer cell aggressiveness. Here, we aimed at identifying RNA-binding proteins (RBPs...
Source: European Journal of Cell Biology - February 24, 2024 Category: Cytology Authors: Julia Rosemann Jonas Pyko Roland Jacob Jana Macho Matthias Kappler Alexander W Eckert Monika Haemmerle Tony Gutschner Source Type: research
Deletion of exons 2 and 3 from Actb and cell immortalization lead to widespread, β-actin independent alterations in gene expression associated with cell cycle control
Eur J Cell Biol. 2024 Feb 15;103(2):151397. doi: 10.1016/j.ejcb.2024.151397. Online ahead of print.ABSTRACTThe cytoplasmic actin proteins, β- and γ-actin, are 99% identical but thought to perform non-redundant functions. The nucleotide coding regions of cytoplasmic actin genes, Actb and Actg1, are 89% identical. Knockout (KO) of Actb by Cre-mediated deletion of first coding exons 2 and 3 in mice is embryonic lethal and fibroblasts derived from KO embryos (MEFs) fail to proliferate. In contrast, Actg1 KO MEFs display with a much milder defect in cell proliferation and Actg1 KO mice are viable, but present with increased p...
Source: European Journal of Cell Biology - February 22, 2024 Category: Cytology Authors: Lauren J Sundby William M Southern Jiao Sun Xiaobai Patrinostro Wei Zhang Jeongsik Yong James M Ervasti Source Type: research
Deletion of exons 2 and 3 from Actb and cell immortalization lead to widespread, β-actin independent alterations in gene expression associated with cell cycle control
Eur J Cell Biol. 2024 Feb 15;103(2):151397. doi: 10.1016/j.ejcb.2024.151397. Online ahead of print.ABSTRACTThe cytoplasmic actin proteins, β- and γ-actin, are 99% identical but thought to perform non-redundant functions. The nucleotide coding regions of cytoplasmic actin genes, Actb and Actg1, are 89% identical. Knockout (KO) of Actb by Cre-mediated deletion of first coding exons 2 and 3 in mice is embryonic lethal and fibroblasts derived from KO embryos (MEFs) fail to proliferate. In contrast, Actg1 KO MEFs display with a much milder defect in cell proliferation and Actg1 KO mice are viable, but present with increased p...
Source: European Journal of Cell Biology - February 22, 2024 Category: Cytology Authors: Lauren J Sundby William M Southern Jiao Sun Xiaobai Patrinostro Wei Zhang Jeongsik Yong James M Ervasti Source Type: research
Molecular mechanisms of naringenin modulation of mitochondrial permeability transition acting on F < sub > 1 < /sub > F < sub > O < /sub > -ATPase and counteracting saline load-induced injury in SHRSP cerebral endothelial cells
Eur J Cell Biol. 2024 Feb 15;103(2):151398. doi: 10.1016/j.ejcb.2024.151398. Online ahead of print.ABSTRACTNaringenin (NRG) was characterized for its ability to counteract mitochondrial dysfunction which is linked to cardiovascular diseases. The F1FO-ATPase can act as a molecular target of NRG. The interaction of NRG with this enzyme can avoid the energy transmission mechanism of ATP hydrolysis, especially in the presence of Ca2+ cation used as cofactor. Indeed, NRG was a selective inhibitor of the hydrophilic F1 domain displaying a binding site overlapped with quercetin in the inside surface of an annulus made by the thre...
Source: European Journal of Cell Biology - February 18, 2024 Category: Cytology Authors: Salvatore Nesci Cristina Algieri Matteo Antonio Tallarida Rosita Stanzione Saverio Marchi Donatella Pietrangelo Fabiana Trombetti Luca D'Ambrosio Maurizio Forte Maria Cotugno Ilaria Nunzi Rachele Bigi Loredana Maiuolo Antonio De Nino Paolo Pinton Giovanni Source Type: research
Molecular mechanisms of naringenin modulation of mitochondrial permeability transition acting on F < sub > 1 < /sub > F < sub > O < /sub > -ATPase and counteracting saline load-induced injury in SHRSP cerebral endothelial cells
Eur J Cell Biol. 2024 Feb 15;103(2):151398. doi: 10.1016/j.ejcb.2024.151398. Online ahead of print.ABSTRACTNaringenin (NRG) was characterized for its ability to counteract mitochondrial dysfunction which is linked to cardiovascular diseases. The F1FO-ATPase can act as a molecular target of NRG. The interaction of NRG with this enzyme can avoid the energy transmission mechanism of ATP hydrolysis, especially in the presence of Ca2+ cation used as cofactor. Indeed, NRG was a selective inhibitor of the hydrophilic F1 domain displaying a binding site overlapped with quercetin in the inside surface of an annulus made by the thre...
Source: European Journal of Cell Biology - February 18, 2024 Category: Cytology Authors: Salvatore Nesci Cristina Algieri Matteo Antonio Tallarida Rosita Stanzione Saverio Marchi Donatella Pietrangelo Fabiana Trombetti Luca D'Ambrosio Maurizio Forte Maria Cotugno Ilaria Nunzi Rachele Bigi Loredana Maiuolo Antonio De Nino Paolo Pinton Giovanni Source Type: research
Molecular mechanisms of naringenin modulation of mitochondrial permeability transition acting on F < sub > 1 < /sub > F < sub > O < /sub > -ATPase and counteracting saline load-induced injury in SHRSP cerebral endothelial cells
Eur J Cell Biol. 2024 Feb 15;103(2):151398. doi: 10.1016/j.ejcb.2024.151398. Online ahead of print.ABSTRACTNaringenin (NRG) was characterized for its ability to counteract mitochondrial dysfunction which is linked to cardiovascular diseases. The F1FO-ATPase can act as a molecular target of NRG. The interaction of NRG with this enzyme can avoid the energy transmission mechanism of ATP hydrolysis, especially in the presence of Ca2+ cation used as cofactor. Indeed, NRG was a selective inhibitor of the hydrophilic F1 domain displaying a binding site overlapped with quercetin in the inside surface of an annulus made by the thre...
Source: European Journal of Cell Biology - February 18, 2024 Category: Cytology Authors: Salvatore Nesci Cristina Algieri Matteo Antonio Tallarida Rosita Stanzione Saverio Marchi Donatella Pietrangelo Fabiana Trombetti Luca D'Ambrosio Maurizio Forte Maria Cotugno Ilaria Nunzi Rachele Bigi Loredana Maiuolo Antonio De Nino Paolo Pinton Giovanni Source Type: research
