Reconstitution of the transition from a lamellipodia- to filopodia-like actin network with purified proteins
Eur J Cell Biol. 2023 Oct 20;102(4):151367. doi: 10.1016/j.ejcb.2023.151367. Online ahead of print.ABSTRACTHow cells utilize complex mixtures of actin binding proteins to assemble and maintain functionally diverse actin filament networks with distinct architectures and dynamics within a common cytoplasm is a longstanding question in cell biology. A compelling example of complex and specialized actin structures in cells are filopodia which sense extracellular chemical and mechanical signals to help steer motile cells. Filopodia have distinct actin architecture, composed of long, parallel actin filaments bundled by fascin, w...
Source: European Journal of Cell Biology - October 27, 2023 Category: Cytology Authors: Cristian Suarez Jonathan D Winkelman Alyssa J Harker Hannah J Ye Patrick M McCall Alisha N Morganthaler Margaret L Gardel David R Kovar Source Type: research
Reconstitution of the transition from a lamellipodia- to filopodia-like actin network with purified proteins
Eur J Cell Biol. 2023 Oct 20;102(4):151367. doi: 10.1016/j.ejcb.2023.151367. Online ahead of print.ABSTRACTHow cells utilize complex mixtures of actin binding proteins to assemble and maintain functionally diverse actin filament networks with distinct architectures and dynamics within a common cytoplasm is a longstanding question in cell biology. A compelling example of complex and specialized actin structures in cells are filopodia which sense extracellular chemical and mechanical signals to help steer motile cells. Filopodia have distinct actin architecture, composed of long, parallel actin filaments bundled by fascin, w...
Source: European Journal of Cell Biology - October 27, 2023 Category: Cytology Authors: Cristian Suarez Jonathan D Winkelman Alyssa J Harker Hannah J Ye Patrick M McCall Alisha N Morganthaler Margaret L Gardel David R Kovar Source Type: research
Reconstitution of the transition from a lamellipodia- to filopodia-like actin network with purified proteins
Eur J Cell Biol. 2023 Oct 20;102(4):151367. doi: 10.1016/j.ejcb.2023.151367. Online ahead of print.ABSTRACTHow cells utilize complex mixtures of actin binding proteins to assemble and maintain functionally diverse actin filament networks with distinct architectures and dynamics within a common cytoplasm is a longstanding question in cell biology. A compelling example of complex and specialized actin structures in cells are filopodia which sense extracellular chemical and mechanical signals to help steer motile cells. Filopodia have distinct actin architecture, composed of long, parallel actin filaments bundled by fascin, w...
Source: European Journal of Cell Biology - October 27, 2023 Category: Cytology Authors: Cristian Suarez Jonathan D Winkelman Alyssa J Harker Hannah J Ye Patrick M McCall Alisha N Morganthaler Margaret L Gardel David R Kovar Source Type: research
Reconstitution of the transition from a lamellipodia- to filopodia-like actin network with purified proteins
Eur J Cell Biol. 2023 Oct 20;102(4):151367. doi: 10.1016/j.ejcb.2023.151367. Online ahead of print.ABSTRACTHow cells utilize complex mixtures of actin binding proteins to assemble and maintain functionally diverse actin filament networks with distinct architectures and dynamics within a common cytoplasm is a longstanding question in cell biology. A compelling example of complex and specialized actin structures in cells are filopodia which sense extracellular chemical and mechanical signals to help steer motile cells. Filopodia have distinct actin architecture, composed of long, parallel actin filaments bundled by fascin, w...
Source: European Journal of Cell Biology - October 27, 2023 Category: Cytology Authors: Cristian Suarez Jonathan D Winkelman Alyssa J Harker Hannah J Ye Patrick M McCall Alisha N Morganthaler Margaret L Gardel David R Kovar Source Type: research
Reconstitution of the transition from a lamellipodia- to filopodia-like actin network with purified proteins
Eur J Cell Biol. 2023 Oct 20;102(4):151367. doi: 10.1016/j.ejcb.2023.151367. Online ahead of print.ABSTRACTHow cells utilize complex mixtures of actin binding proteins to assemble and maintain functionally diverse actin filament networks with distinct architectures and dynamics within a common cytoplasm is a longstanding question in cell biology. A compelling example of complex and specialized actin structures in cells are filopodia which sense extracellular chemical and mechanical signals to help steer motile cells. Filopodia have distinct actin architecture, composed of long, parallel actin filaments bundled by fascin, w...
Source: European Journal of Cell Biology - October 27, 2023 Category: Cytology Authors: Cristian Suarez Jonathan D Winkelman Alyssa J Harker Hannah J Ye Patrick M McCall Alisha N Morganthaler Margaret L Gardel David R Kovar Source Type: research
Microtubule rescue control by drugs and MAPs examined with in vitro pedestal assay
Eur J Cell Biol. 2023 Oct 17;102(4):151366. doi: 10.1016/j.ejcb.2023.151366. Online ahead of print.ABSTRACTMicrotubules are essential cytoskeletal polymers, which exhibit stochastic transitions between assembly and disassembly, known as catastrophes and rescues. Understanding of catastrophes, rescues, and their control by drugs and microtubule associated proteins (MAPs) has been informed by in vitro reconstitutions of microtubule dynamics. In such experiments microtubules are typically observed on a flat surface of the coverslip. In contrast, we have recently proposed a modified setup in which microtubules assemble from st...
Source: European Journal of Cell Biology - October 23, 2023 Category: Cytology Authors: Mikhail N Anisimov Alena V Korshunova Vladimir V Popov Nikita B Gudimchuk Source Type: research
Microtubule rescue control by drugs and MAPs examined with in vitro pedestal assay
Eur J Cell Biol. 2023 Oct 17;102(4):151366. doi: 10.1016/j.ejcb.2023.151366. Online ahead of print.ABSTRACTMicrotubules are essential cytoskeletal polymers, which exhibit stochastic transitions between assembly and disassembly, known as catastrophes and rescues. Understanding of catastrophes, rescues, and their control by drugs and microtubule associated proteins (MAPs) has been informed by in vitro reconstitutions of microtubule dynamics. In such experiments microtubules are typically observed on a flat surface of the coverslip. In contrast, we have recently proposed a modified setup in which microtubules assemble from st...
Source: European Journal of Cell Biology - October 23, 2023 Category: Cytology Authors: Mikhail N Anisimov Alena V Korshunova Vladimir V Popov Nikita B Gudimchuk Source Type: research
Microtubule rescue control by drugs and MAPs examined with in vitro pedestal assay
Eur J Cell Biol. 2023 Oct 17;102(4):151366. doi: 10.1016/j.ejcb.2023.151366. Online ahead of print.ABSTRACTMicrotubules are essential cytoskeletal polymers, which exhibit stochastic transitions between assembly and disassembly, known as catastrophes and rescues. Understanding of catastrophes, rescues, and their control by drugs and microtubule associated proteins (MAPs) has been informed by in vitro reconstitutions of microtubule dynamics. In such experiments microtubules are typically observed on a flat surface of the coverslip. In contrast, we have recently proposed a modified setup in which microtubules assemble from st...
Source: European Journal of Cell Biology - October 23, 2023 Category: Cytology Authors: Mikhail N Anisimov Alena V Korshunova Vladimir V Popov Nikita B Gudimchuk Source Type: research
Microtubule rescue control by drugs and MAPs examined with in vitro pedestal assay
Eur J Cell Biol. 2023 Oct 17;102(4):151366. doi: 10.1016/j.ejcb.2023.151366. Online ahead of print.ABSTRACTMicrotubules are essential cytoskeletal polymers, which exhibit stochastic transitions between assembly and disassembly, known as catastrophes and rescues. Understanding of catastrophes, rescues, and their control by drugs and microtubule associated proteins (MAPs) has been informed by in vitro reconstitutions of microtubule dynamics. In such experiments microtubules are typically observed on a flat surface of the coverslip. In contrast, we have recently proposed a modified setup in which microtubules assemble from st...
Source: European Journal of Cell Biology - October 23, 2023 Category: Cytology Authors: Mikhail N Anisimov Alena V Korshunova Vladimir V Popov Nikita B Gudimchuk Source Type: research
Microtubule rescue control by drugs and MAPs examined with in vitro pedestal assay
Eur J Cell Biol. 2023 Oct 17;102(4):151366. doi: 10.1016/j.ejcb.2023.151366. Online ahead of print.ABSTRACTMicrotubules are essential cytoskeletal polymers, which exhibit stochastic transitions between assembly and disassembly, known as catastrophes and rescues. Understanding of catastrophes, rescues, and their control by drugs and microtubule associated proteins (MAPs) has been informed by in vitro reconstitutions of microtubule dynamics. In such experiments microtubules are typically observed on a flat surface of the coverslip. In contrast, we have recently proposed a modified setup in which microtubules assemble from st...
Source: European Journal of Cell Biology - October 23, 2023 Category: Cytology Authors: Mikhail N Anisimov Alena V Korshunova Vladimir V Popov Nikita B Gudimchuk Source Type: research
Call to action to properly utilize electron microscopy to measure organelles to monitor disease
Eur J Cell Biol. 2023 Oct 16;102(4):151365. doi: 10.1016/j.ejcb.2023.151365. Online ahead of print.ABSTRACTThis review provides an overview of the current methods for quantifying mitochondrial ultrastructure, including cristae morphology, mitochondrial contact sites, and recycling machinery and a guide to utilizing electron microscopy to effectively measure these organelles. Quantitative analysis of mitochondrial ultrastructure is essential for understanding mitochondrial biology and developing therapeutic strategies for mitochondrial-related diseases. Techniques such as transmission electron microscopy (TEM) and serial bl...
Source: European Journal of Cell Biology - October 21, 2023 Category: Cytology Authors: Kit Neikirk Edgar-Garza Lopez Andrea G Marshall Ahmad Alghanem Evan Krystofiak Bartosz Kula Nathan Smith Jianqiang Shao Prasanna Katti Antentor Hinton Source Type: research
Call to action to properly utilize electron microscopy to measure organelles to monitor disease
Eur J Cell Biol. 2023 Oct 16;102(4):151365. doi: 10.1016/j.ejcb.2023.151365. Online ahead of print.ABSTRACTThis review provides an overview of the current methods for quantifying mitochondrial ultrastructure, including cristae morphology, mitochondrial contact sites, and recycling machinery and a guide to utilizing electron microscopy to effectively measure these organelles. Quantitative analysis of mitochondrial ultrastructure is essential for understanding mitochondrial biology and developing therapeutic strategies for mitochondrial-related diseases. Techniques such as transmission electron microscopy (TEM) and serial bl...
Source: European Journal of Cell Biology - October 21, 2023 Category: Cytology Authors: Kit Neikirk Edgar-Garza Lopez Andrea G Marshall Ahmad Alghanem Evan Krystofiak Bartosz Kula Nathan Smith Jianqiang Shao Prasanna Katti Antentor Hinton Source Type: research
A bacterial effector protein promotes nuclear translocation of Stat3 to induce IL-10
Eur J Cell Biol. 2023 Oct 2;102(4):151364. doi: 10.1016/j.ejcb.2023.151364. Online ahead of print.ABSTRACTThe multifunctional Yersinia effector YopM inhibits effector triggered immunity and increases production of the anti-inflammatory cytokine Interleukin-10 (IL-10) to suppress the host immune response. Previously it was shown that YopM induces IL-10 gene expression by elevating phosphorylation of the serine-threonine kinase RSK1 in the nucleus of human macrophages. Using transcriptomics, we found that YopM strongly affects expression of genes belonging to the JAK-STAT signaling pathway. Further analysis revealed that Yop...
Source: European Journal of Cell Biology - October 8, 2023 Category: Cytology Authors: Laura Berneking Indra Bekere S ören Rob Marie Schnapp Jiabin Huang Klaus Ruckdeschel Martin Aepfelbacher Source Type: research
A bacterial effector protein promotes nuclear translocation of Stat3 to induce IL-10
Eur J Cell Biol. 2023 Oct 2;102(4):151364. doi: 10.1016/j.ejcb.2023.151364. Online ahead of print.ABSTRACTThe multifunctional Yersinia effector YopM inhibits effector triggered immunity and increases production of the anti-inflammatory cytokine Interleukin-10 (IL-10) to suppress the host immune response. Previously it was shown that YopM induces IL-10 gene expression by elevating phosphorylation of the serine-threonine kinase RSK1 in the nucleus of human macrophages. Using transcriptomics, we found that YopM strongly affects expression of genes belonging to the JAK-STAT signaling pathway. Further analysis revealed that Yop...
Source: European Journal of Cell Biology - October 8, 2023 Category: Cytology Authors: Laura Berneking Indra Bekere S ören Rob Marie Schnapp Jiabin Huang Klaus Ruckdeschel Martin Aepfelbacher Source Type: research
A bacterial effector protein promotes nuclear translocation of Stat3 to induce IL-10
Eur J Cell Biol. 2023 Oct 2;102(4):151364. doi: 10.1016/j.ejcb.2023.151364. Online ahead of print.ABSTRACTThe multifunctional Yersinia effector YopM inhibits effector triggered immunity and increases production of the anti-inflammatory cytokine Interleukin-10 (IL-10) to suppress the host immune response. Previously it was shown that YopM induces IL-10 gene expression by elevating phosphorylation of the serine-threonine kinase RSK1 in the nucleus of human macrophages. Using transcriptomics, we found that YopM strongly affects expression of genes belonging to the JAK-STAT signaling pathway. Further analysis revealed that Yop...
Source: European Journal of Cell Biology - October 8, 2023 Category: Cytology Authors: Laura Berneking Indra Bekere S ören Rob Marie Schnapp Jiabin Huang Klaus Ruckdeschel Martin Aepfelbacher Source Type: research
