Extracellular vesicles on the move: Traversing the complex matrix of tissues
Eur J Cell Biol. 2023 Nov 11;102(4):151372. doi: 10.1016/j.ejcb.2023.151372. Online ahead of print.ABSTRACTExtracellular vesicles are small particles involved in intercellular signaling. They are produced by virtually all cell types, transport biological molecules, and are released into the extracellular space. Studies on extracellular vesicles have become more numerous in recent years, leading to promising research on their potential impact on health and disease. Despite significant progress in understanding the bioactivity of extracellular vesicles, most in vitro and in vivo studies overlook their transport through the e...
Source: European Journal of Cell Biology - November 16, 2023 Category: Cytology Authors: Syrine Arif V éronique J Moulin Source Type: research
MitoTracker: A useful tool in need of better alternatives
Eur J Cell Biol. 2023 Oct 31;102(4):151371. doi: 10.1016/j.ejcb.2023.151371. Online ahead of print.ABSTRACTThe fluorescence viewing of mitochondria is commonly performed by MitoTracker, a lipophilic cationic dye that is taken up by the mitochondria. In this forum, we highlight several issues that may occur with MitoTracker, including staining of other organelles. Our aim is to offer alternative dyes and discuss their advantages and disadvantages. We also offer options for software with alternatives to MitoTracker to expedite future experimental design.PMID:37956476 | DOI:10.1016/j.ejcb.2023.151371 (Source: European Journal of Cell Biology)
Source: European Journal of Cell Biology - November 13, 2023 Category: Cytology Authors: Kit Neikirk Andrea G Marshall Bartosz Kula Nathan Smith Sharonda LeBlanc Antentor Hinton Source Type: research
MitoTracker: A useful tool in need of better alternatives
Eur J Cell Biol. 2023 Oct 31;102(4):151371. doi: 10.1016/j.ejcb.2023.151371. Online ahead of print.ABSTRACTThe fluorescence viewing of mitochondria is commonly performed by MitoTracker, a lipophilic cationic dye that is taken up by the mitochondria. In this forum, we highlight several issues that may occur with MitoTracker, including staining of other organelles. Our aim is to offer alternative dyes and discuss their advantages and disadvantages. We also offer options for software with alternatives to MitoTracker to expedite future experimental design.PMID:37956476 | DOI:10.1016/j.ejcb.2023.151371 (Source: European Journal of Cell Biology)
Source: European Journal of Cell Biology - November 13, 2023 Category: Cytology Authors: Kit Neikirk Andrea G Marshall Bartosz Kula Nathan Smith Sharonda LeBlanc Antentor Hinton Source Type: research
MitoTracker: A useful tool in need of better alternatives
Eur J Cell Biol. 2023 Oct 31;102(4):151371. doi: 10.1016/j.ejcb.2023.151371. Online ahead of print.ABSTRACTThe fluorescence viewing of mitochondria is commonly performed by MitoTracker, a lipophilic cationic dye that is taken up by the mitochondria. In this forum, we highlight several issues that may occur with MitoTracker, including staining of other organelles. Our aim is to offer alternative dyes and discuss their advantages and disadvantages. We also offer options for software with alternatives to MitoTracker to expedite future experimental design.PMID:37956476 | DOI:10.1016/j.ejcb.2023.151371 (Source: European Journal of Cell Biology)
Source: European Journal of Cell Biology - November 13, 2023 Category: Cytology Authors: Kit Neikirk Andrea G Marshall Bartosz Kula Nathan Smith Sharonda LeBlanc Antentor Hinton Source Type: research
MitoTracker: A useful tool in need of better alternatives
Eur J Cell Biol. 2023 Oct 31;102(4):151371. doi: 10.1016/j.ejcb.2023.151371. Online ahead of print.ABSTRACTThe fluorescence viewing of mitochondria is commonly performed by MitoTracker, a lipophilic cationic dye that is taken up by the mitochondria. In this forum, we highlight several issues that may occur with MitoTracker, including staining of other organelles. Our aim is to offer alternative dyes and discuss their advantages and disadvantages. We also offer options for software with alternatives to MitoTracker to expedite future experimental design.PMID:37956476 | DOI:10.1016/j.ejcb.2023.151371 (Source: European Journal of Cell Biology)
Source: European Journal of Cell Biology - November 13, 2023 Category: Cytology Authors: Kit Neikirk Andrea G Marshall Bartosz Kula Nathan Smith Sharonda LeBlanc Antentor Hinton Source Type: research
Modelling Legionnaires' disease: Lessons learned from invertebrate and vertebrate animal models
Eur J Cell Biol. 2023 Oct 31;102(4):151369. doi: 10.1016/j.ejcb.2023.151369. Online ahead of print.ABSTRACTThe study of virulence of Legionella pneumophila and its interactions with its hosts has been predominantly conducted in cellulo in the past decades. Although easy to implement and allowing the dissection of molecular pathways underlying host-pathogen interactions, these cellular models fail to provide conditions of the complex environments encountered by the bacteria during the infection of multicellular organisms. To improve our understanding of human infection, several animal models have been developed. This review...
Source: European Journal of Cell Biology - November 5, 2023 Category: Cytology Authors: Clarisse Leseigneur Carmen Buchrieser Source Type: research
Modelling Legionnaires' disease: Lessons learned from invertebrate and vertebrate animal models
Eur J Cell Biol. 2023 Oct 31;102(4):151369. doi: 10.1016/j.ejcb.2023.151369. Online ahead of print.ABSTRACTThe study of virulence of Legionella pneumophila and its interactions with its hosts has been predominantly conducted in cellulo in the past decades. Although easy to implement and allowing the dissection of molecular pathways underlying host-pathogen interactions, these cellular models fail to provide conditions of the complex environments encountered by the bacteria during the infection of multicellular organisms. To improve our understanding of human infection, several animal models have been developed. This review...
Source: European Journal of Cell Biology - November 5, 2023 Category: Cytology Authors: Clarisse Leseigneur Carmen Buchrieser Source Type: research
Modelling Legionnaires' disease: Lessons learned from invertebrate and vertebrate animal models
Eur J Cell Biol. 2023 Oct 31;102(4):151369. doi: 10.1016/j.ejcb.2023.151369. Online ahead of print.ABSTRACTThe study of virulence of Legionella pneumophila and its interactions with its hosts has been predominantly conducted in cellulo in the past decades. Although easy to implement and allowing the dissection of molecular pathways underlying host-pathogen interactions, these cellular models fail to provide conditions of the complex environments encountered by the bacteria during the infection of multicellular organisms. To improve our understanding of human infection, several animal models have been developed. This review...
Source: European Journal of Cell Biology - November 5, 2023 Category: Cytology Authors: Clarisse Leseigneur Carmen Buchrieser Source Type: research
Modelling Legionnaires' disease: Lessons learned from invertebrate and vertebrate animal models
Eur J Cell Biol. 2023 Oct 31;102(4):151369. doi: 10.1016/j.ejcb.2023.151369. Online ahead of print.ABSTRACTThe study of virulence of Legionella pneumophila and its interactions with its hosts has been predominantly conducted in cellulo in the past decades. Although easy to implement and allowing the dissection of molecular pathways underlying host-pathogen interactions, these cellular models fail to provide conditions of the complex environments encountered by the bacteria during the infection of multicellular organisms. To improve our understanding of human infection, several animal models have been developed. This review...
Source: European Journal of Cell Biology - November 5, 2023 Category: Cytology Authors: Clarisse Leseigneur Carmen Buchrieser Source Type: research
Modelling Legionnaires' disease: Lessons learned from invertebrate and vertebrate animal models
Eur J Cell Biol. 2023 Oct 31;102(4):151369. doi: 10.1016/j.ejcb.2023.151369. Online ahead of print.ABSTRACTThe study of virulence of Legionella pneumophila and its interactions with its hosts has been predominantly conducted in cellulo in the past decades. Although easy to implement and allowing the dissection of molecular pathways underlying host-pathogen interactions, these cellular models fail to provide conditions of the complex environments encountered by the bacteria during the infection of multicellular organisms. To improve our understanding of human infection, several animal models have been developed. This review...
Source: European Journal of Cell Biology - November 5, 2023 Category: Cytology Authors: Clarisse Leseigneur Carmen Buchrieser Source Type: research
Modelling Legionnaires' disease: Lessons learned from invertebrate and vertebrate animal models
Eur J Cell Biol. 2023 Oct 31;102(4):151369. doi: 10.1016/j.ejcb.2023.151369. Online ahead of print.ABSTRACTThe study of virulence of Legionella pneumophila and its interactions with its hosts has been predominantly conducted in cellulo in the past decades. Although easy to implement and allowing the dissection of molecular pathways underlying host-pathogen interactions, these cellular models fail to provide conditions of the complex environments encountered by the bacteria during the infection of multicellular organisms. To improve our understanding of human infection, several animal models have been developed. This review...
Source: European Journal of Cell Biology - November 5, 2023 Category: Cytology Authors: Clarisse Leseigneur Carmen Buchrieser Source Type: research
Beyond uniformity: Exploring the heterogeneous and dynamic nature of the microtubule lattice
Eur J Cell Biol. 2023 Oct 29;102(4):151370. doi: 10.1016/j.ejcb.2023.151370. Online ahead of print.ABSTRACTA fair amount of research on microtubules since their discovery in 1963 has focused on their dynamic tips. In contrast, the microtubule lattice was long believed to be highly regular and static, and consequently received far less attention. Yet, as it turned out, the microtubule lattice is neither as regular, nor as static as previously believed: structural studies uncovered the remarkable wealth of different conformations the lattice can accommodate. In the last decade, the microtubule lattice was shown to be labile ...
Source: European Journal of Cell Biology - November 3, 2023 Category: Cytology Authors: Mariana Romeiro Motta Subham Biswas Laura Schaedel Source Type: research
Reconstitution of actin-based cellular processes: Why encapsulation changes the rules
Eur J Cell Biol. 2023 Oct 30;102(4):151368. doi: 10.1016/j.ejcb.2023.151368. Online ahead of print.ABSTRACTWhile in vitro reconstitution of cellular processes is progressing rapidly, the encapsulation of biomimetic systems to reproduce the cellular environment is a major challenge. Here we review the difficulties, using reconstitution of processes dependent on actin polymerization as an example. Some of the problems are purely technical, due to the need for engineering strategies to encapsulate concentrated solutions in micrometer-sized compartments. However, other significant issues arise from the reduction of experimenta...
Source: European Journal of Cell Biology - November 3, 2023 Category: Cytology Authors: Fabina Binth Kandiyoth Alph ée Michelot Source Type: research
Reconstitution of the transition from a lamellipodia- to filopodia-like actin network with purified proteins
Eur J Cell Biol. 2023 Oct 20;102(4):151367. doi: 10.1016/j.ejcb.2023.151367. Online ahead of print.ABSTRACTHow cells utilize complex mixtures of actin binding proteins to assemble and maintain functionally diverse actin filament networks with distinct architectures and dynamics within a common cytoplasm is a longstanding question in cell biology. A compelling example of complex and specialized actin structures in cells are filopodia which sense extracellular chemical and mechanical signals to help steer motile cells. Filopodia have distinct actin architecture, composed of long, parallel actin filaments bundled by fascin, w...
Source: European Journal of Cell Biology - October 27, 2023 Category: Cytology Authors: Cristian Suarez Jonathan D Winkelman Alyssa J Harker Hannah J Ye Patrick M McCall Alisha N Morganthaler Margaret L Gardel David R Kovar Source Type: research
Reconstitution of the transition from a lamellipodia- to filopodia-like actin network with purified proteins
Eur J Cell Biol. 2023 Oct 20;102(4):151367. doi: 10.1016/j.ejcb.2023.151367. Online ahead of print.ABSTRACTHow cells utilize complex mixtures of actin binding proteins to assemble and maintain functionally diverse actin filament networks with distinct architectures and dynamics within a common cytoplasm is a longstanding question in cell biology. A compelling example of complex and specialized actin structures in cells are filopodia which sense extracellular chemical and mechanical signals to help steer motile cells. Filopodia have distinct actin architecture, composed of long, parallel actin filaments bundled by fascin, w...
Source: European Journal of Cell Biology - October 27, 2023 Category: Cytology Authors: Cristian Suarez Jonathan D Winkelman Alyssa J Harker Hannah J Ye Patrick M McCall Alisha N Morganthaler Margaret L Gardel David R Kovar Source Type: research
