A bacterial effector protein promotes nuclear translocation of Stat3 to induce IL-10
Eur J Cell Biol. 2023 Oct 2;102(4):151364. doi: 10.1016/j.ejcb.2023.151364. Online ahead of print.ABSTRACTThe multifunctional Yersinia effector YopM inhibits effector triggered immunity and increases production of the anti-inflammatory cytokine Interleukin-10 (IL-10) to suppress the host immune response. Previously it was shown that YopM induces IL-10 gene expression by elevating phosphorylation of the serine-threonine kinase RSK1 in the nucleus of human macrophages. Using transcriptomics, we found that YopM strongly affects expression of genes belonging to the JAK-STAT signaling pathway. Further analysis revealed that Yop...
Source: European Journal of Cell Biology - October 8, 2023 Category: Cytology Authors: Laura Berneking Indra Bekere S ören Rob Marie Schnapp Jiabin Huang Klaus Ruckdeschel Martin Aepfelbacher Source Type: research
A bacterial effector protein promotes nuclear translocation of Stat3 to induce IL-10
Eur J Cell Biol. 2023 Oct 2;102(4):151364. doi: 10.1016/j.ejcb.2023.151364. Online ahead of print.ABSTRACTThe multifunctional Yersinia effector YopM inhibits effector triggered immunity and increases production of the anti-inflammatory cytokine Interleukin-10 (IL-10) to suppress the host immune response. Previously it was shown that YopM induces IL-10 gene expression by elevating phosphorylation of the serine-threonine kinase RSK1 in the nucleus of human macrophages. Using transcriptomics, we found that YopM strongly affects expression of genes belonging to the JAK-STAT signaling pathway. Further analysis revealed that Yop...
Source: European Journal of Cell Biology - October 8, 2023 Category: Cytology Authors: Laura Berneking Indra Bekere S ören Rob Marie Schnapp Jiabin Huang Klaus Ruckdeschel Martin Aepfelbacher Source Type: research
A bacterial effector protein promotes nuclear translocation of Stat3 to induce IL-10
Eur J Cell Biol. 2023 Oct 2;102(4):151364. doi: 10.1016/j.ejcb.2023.151364. Online ahead of print.ABSTRACTThe multifunctional Yersinia effector YopM inhibits effector triggered immunity and increases production of the anti-inflammatory cytokine Interleukin-10 (IL-10) to suppress the host immune response. Previously it was shown that YopM induces IL-10 gene expression by elevating phosphorylation of the serine-threonine kinase RSK1 in the nucleus of human macrophages. Using transcriptomics, we found that YopM strongly affects expression of genes belonging to the JAK-STAT signaling pathway. Further analysis revealed that Yop...
Source: European Journal of Cell Biology - October 8, 2023 Category: Cytology Authors: Laura Berneking Indra Bekere S ören Rob Marie Schnapp Jiabin Huang Klaus Ruckdeschel Martin Aepfelbacher Source Type: research
A bacterial effector protein promotes nuclear translocation of Stat3 to induce IL-10
Eur J Cell Biol. 2023 Oct 2;102(4):151364. doi: 10.1016/j.ejcb.2023.151364. Online ahead of print.ABSTRACTThe multifunctional Yersinia effector YopM inhibits effector triggered immunity and increases production of the anti-inflammatory cytokine Interleukin-10 (IL-10) to suppress the host immune response. Previously it was shown that YopM induces IL-10 gene expression by elevating phosphorylation of the serine-threonine kinase RSK1 in the nucleus of human macrophages. Using transcriptomics, we found that YopM strongly affects expression of genes belonging to the JAK-STAT signaling pathway. Further analysis revealed that Yop...
Source: European Journal of Cell Biology - October 8, 2023 Category: Cytology Authors: Laura Berneking Indra Bekere S ören Rob Marie Schnapp Jiabin Huang Klaus Ruckdeschel Martin Aepfelbacher Source Type: research
A bacterial effector protein promotes nuclear translocation of Stat3 to induce IL-10
Eur J Cell Biol. 2023 Oct 2;102(4):151364. doi: 10.1016/j.ejcb.2023.151364. Online ahead of print.ABSTRACTThe multifunctional Yersinia effector YopM inhibits effector triggered immunity and increases production of the anti-inflammatory cytokine Interleukin-10 (IL-10) to suppress the host immune response. Previously it was shown that YopM induces IL-10 gene expression by elevating phosphorylation of the serine-threonine kinase RSK1 in the nucleus of human macrophages. Using transcriptomics, we found that YopM strongly affects expression of genes belonging to the JAK-STAT signaling pathway. Further analysis revealed that Yop...
Source: European Journal of Cell Biology - October 8, 2023 Category: Cytology Authors: Laura Berneking Indra Bekere S ören Rob Marie Schnapp Jiabin Huang Klaus Ruckdeschel Martin Aepfelbacher Source Type: research
A bacterial effector protein promotes nuclear translocation of Stat3 to induce IL-10
Eur J Cell Biol. 2023 Oct 2;102(4):151364. doi: 10.1016/j.ejcb.2023.151364. Online ahead of print.ABSTRACTThe multifunctional Yersinia effector YopM inhibits effector triggered immunity and increases production of the anti-inflammatory cytokine Interleukin-10 (IL-10) to suppress the host immune response. Previously it was shown that YopM induces IL-10 gene expression by elevating phosphorylation of the serine-threonine kinase RSK1 in the nucleus of human macrophages. Using transcriptomics, we found that YopM strongly affects expression of genes belonging to the JAK-STAT signaling pathway. Further analysis revealed that Yop...
Source: European Journal of Cell Biology - October 8, 2023 Category: Cytology Authors: Laura Berneking Indra Bekere S ören Rob Marie Schnapp Jiabin Huang Klaus Ruckdeschel Martin Aepfelbacher Source Type: research
Purification of modified mammalian actin isoforms for in vitro reconstitution assays
Eur J Cell Biol. 2023 Sep 28;102(4):151363. doi: 10.1016/j.ejcb.2023.151363. Online ahead of print.ABSTRACTIn vitro reconstitution assays using purified actin have greatly improved our understanding of cytoskeletal dynamics and their regulation by actin-binding proteins. However, early purification methods consisted of harsh conditions to obtain pure actin and often did not include correct maturation and obligate modification of the isolated actin monomers. Novel insights into the folding requirements and N-terminal processing of actin as well as a better understanding of the interaction of actin with monomer sequestering ...
Source: European Journal of Cell Biology - October 1, 2023 Category: Cytology Authors: David J Kast Silvia Jansen Source Type: research
Purification of modified mammalian actin isoforms for in vitro reconstitution assays
Eur J Cell Biol. 2023 Sep 28;102(4):151363. doi: 10.1016/j.ejcb.2023.151363. Online ahead of print.ABSTRACTIn vitro reconstitution assays using purified actin have greatly improved our understanding of cytoskeletal dynamics and their regulation by actin-binding proteins. However, early purification methods consisted of harsh conditions to obtain pure actin and often did not include correct maturation and obligate modification of the isolated actin monomers. Novel insights into the folding requirements and N-terminal processing of actin as well as a better understanding of the interaction of actin with monomer sequestering ...
Source: European Journal of Cell Biology - October 1, 2023 Category: Cytology Authors: David J Kast Silvia Jansen Source Type: research
Purification of modified mammalian actin isoforms for in vitro reconstitution assays
Eur J Cell Biol. 2023 Sep 28;102(4):151363. doi: 10.1016/j.ejcb.2023.151363. Online ahead of print.ABSTRACTIn vitro reconstitution assays using purified actin have greatly improved our understanding of cytoskeletal dynamics and their regulation by actin-binding proteins. However, early purification methods consisted of harsh conditions to obtain pure actin and often did not include correct maturation and obligate modification of the isolated actin monomers. Novel insights into the folding requirements and N-terminal processing of actin as well as a better understanding of the interaction of actin with monomer sequestering ...
Source: European Journal of Cell Biology - October 1, 2023 Category: Cytology Authors: David J Kast Silvia Jansen Source Type: research
Purification of modified mammalian actin isoforms for in vitro reconstitution assays
Eur J Cell Biol. 2023 Sep 28;102(4):151363. doi: 10.1016/j.ejcb.2023.151363. Online ahead of print.ABSTRACTIn vitro reconstitution assays using purified actin have greatly improved our understanding of cytoskeletal dynamics and their regulation by actin-binding proteins. However, early purification methods consisted of harsh conditions to obtain pure actin and often did not include correct maturation and obligate modification of the isolated actin monomers. Novel insights into the folding requirements and N-terminal processing of actin as well as a better understanding of the interaction of actin with monomer sequestering ...
Source: European Journal of Cell Biology - October 1, 2023 Category: Cytology Authors: David J Kast Silvia Jansen Source Type: research
Purification of modified mammalian actin isoforms for in vitro reconstitution assays
Eur J Cell Biol. 2023 Sep 28;102(4):151363. doi: 10.1016/j.ejcb.2023.151363. Online ahead of print.ABSTRACTIn vitro reconstitution assays using purified actin have greatly improved our understanding of cytoskeletal dynamics and their regulation by actin-binding proteins. However, early purification methods consisted of harsh conditions to obtain pure actin and often did not include correct maturation and obligate modification of the isolated actin monomers. Novel insights into the folding requirements and N-terminal processing of actin as well as a better understanding of the interaction of actin with monomer sequestering ...
Source: European Journal of Cell Biology - October 1, 2023 Category: Cytology Authors: David J Kast Silvia Jansen Source Type: research
Purification of modified mammalian actin isoforms for in vitro reconstitution assays
Eur J Cell Biol. 2023 Sep 28;102(4):151363. doi: 10.1016/j.ejcb.2023.151363. Online ahead of print.ABSTRACTIn vitro reconstitution assays using purified actin have greatly improved our understanding of cytoskeletal dynamics and their regulation by actin-binding proteins. However, early purification methods consisted of harsh conditions to obtain pure actin and often did not include correct maturation and obligate modification of the isolated actin monomers. Novel insights into the folding requirements and N-terminal processing of actin as well as a better understanding of the interaction of actin with monomer sequestering ...
Source: European Journal of Cell Biology - October 1, 2023 Category: Cytology Authors: David J Kast Silvia Jansen Source Type: research
Concept of lipid droplet biogenesis
Eur J Cell Biol. 2023 Sep 19;102(4):151362. doi: 10.1016/j.ejcb.2023.151362. Online ahead of print.ABSTRACTLipid droplets (LD) are functionally conserved fat storage organelles found in all cell types. LDs have a unique structure comprising of a hydrophobic core of neutral lipids (fat), triacylglycerol (TAG) and cholesterol esters (CE) surrounded by a phospholipid monolayer. LD surface is decorated by a multitude of proteins and enzymes rendering this compartment functional. Accumulating evidence suggests that LDs originate from discrete ER-subdomains, demarcated by the lipodystrophy protein seipin, however, the mechanisms...
Source: European Journal of Cell Biology - September 24, 2023 Category: Cytology Authors: R Mankamna Kumari Amit Khatri Ritika Chaudhary Vineet Choudhary Source Type: research
Loss of mfsd8 alters the secretome during Dictyostelium aggregation
Eur J Cell Biol. 2023 Sep 19;102(4):151361. doi: 10.1016/j.ejcb.2023.151361. Online ahead of print.ABSTRACTMajor facilitator superfamily domain-containing protein 8 (MFSD8) is a transmembrane protein that has been reported to function as a lysosomal chloride channel. In humans, homozygous mutations in MFSD8 cause a late-infantile form of neuronal ceroid lipofuscinosis (NCL) called CLN7 disease. In the social amoeba Dictyostelium discoideum, Mfsd8 localizes to cytoplasmic puncta and vesicles, and regulates conserved processes during the organism's life cycle. Here, we used D. discoideum to examine the effect of mfsd8-defici...
Source: European Journal of Cell Biology - September 24, 2023 Category: Cytology Authors: Robert J Huber Joshua Gray William D Kim Source Type: research
Concept of lipid droplet biogenesis
Eur J Cell Biol. 2023 Sep 19;102(4):151362. doi: 10.1016/j.ejcb.2023.151362. Online ahead of print.ABSTRACTLipid droplets (LD) are functionally conserved fat storage organelles found in all cell types. LDs have a unique structure comprising of a hydrophobic core of neutral lipids (fat), triacylglycerol (TAG) and cholesterol esters (CE) surrounded by a phospholipid monolayer. LD surface is decorated by a multitude of proteins and enzymes rendering this compartment functional. Accumulating evidence suggests that LDs originate from discrete ER-subdomains, demarcated by the lipodystrophy protein seipin, however, the mechanisms...
Source: European Journal of Cell Biology - September 24, 2023 Category: Cytology Authors: R Mankamna Kumari Amit Khatri Ritika Chaudhary Vineet Choudhary Source Type: research
