Direct observation of cortactin protecting Arp2/3-actin filament branch junctions from GMF-mediated destabilization
Eur J Cell Biol. 2023 Dec 5;103(1):151378. doi: 10.1016/j.ejcb.2023.151378. Online ahead of print.ABSTRACTHow cells tightly control the formation and turnover of branched actin filament arrays to drive cell motility, endocytosis, and other cellular processes is still not well understood. Here, we investigated the mechanistic relationship between two binding partners of the Arp2/3 complex, glia maturation factor (GMF) and cortactin. Individually, GMF and cortactin have opposite effects on the stability of actin filament branches, but it is unknown how they work in concert with each other to govern branch turnover. Using TIR...
Source: European Journal of Cell Biology - December 10, 2023 Category: Cytology Authors: Emma R McGuirk Neha Koundinya Priyashree Nagarajan Shae B Padrick Bruce L Goode Source Type: research
Direct observation of cortactin protecting Arp2/3-actin filament branch junctions from GMF-mediated destabilization
Eur J Cell Biol. 2023 Dec 5;103(1):151378. doi: 10.1016/j.ejcb.2023.151378. Online ahead of print.ABSTRACTHow cells tightly control the formation and turnover of branched actin filament arrays to drive cell motility, endocytosis, and other cellular processes is still not well understood. Here, we investigated the mechanistic relationship between two binding partners of the Arp2/3 complex, glia maturation factor (GMF) and cortactin. Individually, GMF and cortactin have opposite effects on the stability of actin filament branches, but it is unknown how they work in concert with each other to govern branch turnover. Using TIR...
Source: European Journal of Cell Biology - December 10, 2023 Category: Cytology Authors: Emma R McGuirk Neha Koundinya Priyashree Nagarajan Shae B Padrick Bruce L Goode Source Type: research
Direct observation of cortactin protecting Arp2/3-actin filament branch junctions from GMF-mediated destabilization
Eur J Cell Biol. 2023 Dec 5;103(1):151378. doi: 10.1016/j.ejcb.2023.151378. Online ahead of print.ABSTRACTHow cells tightly control the formation and turnover of branched actin filament arrays to drive cell motility, endocytosis, and other cellular processes is still not well understood. Here, we investigated the mechanistic relationship between two binding partners of the Arp2/3 complex, glia maturation factor (GMF) and cortactin. Individually, GMF and cortactin have opposite effects on the stability of actin filament branches, but it is unknown how they work in concert with each other to govern branch turnover. Using TIR...
Source: European Journal of Cell Biology - December 10, 2023 Category: Cytology Authors: Emma R McGuirk Neha Koundinya Priyashree Nagarajan Shae B Padrick Bruce L Goode Source Type: research
Direct observation of cortactin protecting Arp2/3-actin filament branch junctions from GMF-mediated destabilization
Eur J Cell Biol. 2023 Dec 5;103(1):151378. doi: 10.1016/j.ejcb.2023.151378. Online ahead of print.ABSTRACTHow cells tightly control the formation and turnover of branched actin filament arrays to drive cell motility, endocytosis, and other cellular processes is still not well understood. Here, we investigated the mechanistic relationship between two binding partners of the Arp2/3 complex, glia maturation factor (GMF) and cortactin. Individually, GMF and cortactin have opposite effects on the stability of actin filament branches, but it is unknown how they work in concert with each other to govern branch turnover. Using TIR...
Source: European Journal of Cell Biology - December 10, 2023 Category: Cytology Authors: Emma R McGuirk Neha Koundinya Priyashree Nagarajan Shae B Padrick Bruce L Goode Source Type: research
Direct observation of cortactin protecting Arp2/3-actin filament branch junctions from GMF-mediated destabilization
Eur J Cell Biol. 2023 Dec 5;103(1):151378. doi: 10.1016/j.ejcb.2023.151378. Online ahead of print.ABSTRACTHow cells tightly control the formation and turnover of branched actin filament arrays to drive cell motility, endocytosis, and other cellular processes is still not well understood. Here, we investigated the mechanistic relationship between two binding partners of the Arp2/3 complex, glia maturation factor (GMF) and cortactin. Individually, GMF and cortactin have opposite effects on the stability of actin filament branches, but it is unknown how they work in concert with each other to govern branch turnover. Using TIR...
Source: European Journal of Cell Biology - December 10, 2023 Category: Cytology Authors: Emma R McGuirk Neha Koundinya Priyashree Nagarajan Shae B Padrick Bruce L Goode Source Type: research
Direct observation of cortactin protecting Arp2/3-actin filament branch junctions from GMF-mediated destabilization
Eur J Cell Biol. 2023 Dec 5;103(1):151378. doi: 10.1016/j.ejcb.2023.151378. Online ahead of print.ABSTRACTHow cells tightly control the formation and turnover of branched actin filament arrays to drive cell motility, endocytosis, and other cellular processes is still not well understood. Here, we investigated the mechanistic relationship between two binding partners of the Arp2/3 complex, glia maturation factor (GMF) and cortactin. Individually, GMF and cortactin have opposite effects on the stability of actin filament branches, but it is unknown how they work in concert with each other to govern branch turnover. Using TIR...
Source: European Journal of Cell Biology - December 10, 2023 Category: Cytology Authors: Emma R McGuirk Neha Koundinya Priyashree Nagarajan Shae B Padrick Bruce L Goode Source Type: research
Direct observation of cortactin protecting Arp2/3-actin filament branch junctions from GMF-mediated destabilization
Eur J Cell Biol. 2023 Dec 5;103(1):151378. doi: 10.1016/j.ejcb.2023.151378. Online ahead of print.ABSTRACTHow cells tightly control the formation and turnover of branched actin filament arrays to drive cell motility, endocytosis, and other cellular processes is still not well understood. Here, we investigated the mechanistic relationship between two binding partners of the Arp2/3 complex, glia maturation factor (GMF) and cortactin. Individually, GMF and cortactin have opposite effects on the stability of actin filament branches, but it is unknown how they work in concert with each other to govern branch turnover. Using TIR...
Source: European Journal of Cell Biology - December 10, 2023 Category: Cytology Authors: Emma R McGuirk Neha Koundinya Priyashree Nagarajan Shae B Padrick Bruce L Goode Source Type: research
Direct observation of cortactin protecting Arp2/3-actin filament branch junctions from GMF-mediated destabilization
Eur J Cell Biol. 2023 Dec 5;103(1):151378. doi: 10.1016/j.ejcb.2023.151378. Online ahead of print.ABSTRACTHow cells tightly control the formation and turnover of branched actin filament arrays to drive cell motility, endocytosis, and other cellular processes is still not well understood. Here, we investigated the mechanistic relationship between two binding partners of the Arp2/3 complex, glia maturation factor (GMF) and cortactin. Individually, GMF and cortactin have opposite effects on the stability of actin filament branches, but it is unknown how they work in concert with each other to govern branch turnover. Using TIR...
Source: European Journal of Cell Biology - December 10, 2023 Category: Cytology Authors: Emma R McGuirk Neha Koundinya Priyashree Nagarajan Shae B Padrick Bruce L Goode Source Type: research
Direct observation of cortactin protecting Arp2/3-actin filament branch junctions from GMF-mediated destabilization
Eur J Cell Biol. 2023 Dec 5;103(1):151378. doi: 10.1016/j.ejcb.2023.151378. Online ahead of print.ABSTRACTHow cells tightly control the formation and turnover of branched actin filament arrays to drive cell motility, endocytosis, and other cellular processes is still not well understood. Here, we investigated the mechanistic relationship between two binding partners of the Arp2/3 complex, glia maturation factor (GMF) and cortactin. Individually, GMF and cortactin have opposite effects on the stability of actin filament branches, but it is unknown how they work in concert with each other to govern branch turnover. Using TIR...
Source: European Journal of Cell Biology - December 10, 2023 Category: Cytology Authors: Emma R McGuirk Neha Koundinya Priyashree Nagarajan Shae B Padrick Bruce L Goode Source Type: research
Impact of heat and cold shock on epigenetics and chromatin structure
In this study, we investigated the epigenetic profiles of human cells subjected to hyperthermia and hypothermia, revealing significant variations. Heat shock primarily led to DNA methylation increments and epigenetic modifications associated with gene expression silencing. In contrast, cold shock presented a complex scenario, with both methylation and demethylation levels increasing, indicating different epigenetic responses to the opposite thermal stresses. These temperature-induced alterations in the epigenome, particularly their impact on chromatin structural organization, represent an understudied area that could offer...
Source: European Journal of Cell Biology - November 28, 2023 Category: Cytology Authors: Claudio Casali Luca Galgano Lorena Zannino Stella Siciliani Margherita Cavallo Giuliano Mazzini Marco Biggiogera Source Type: research
Impact of heat and cold shock on epigenetics and chromatin structure
In this study, we investigated the epigenetic profiles of human cells subjected to hyperthermia and hypothermia, revealing significant variations. Heat shock primarily led to DNA methylation increments and epigenetic modifications associated with gene expression silencing. In contrast, cold shock presented a complex scenario, with both methylation and demethylation levels increasing, indicating different epigenetic responses to the opposite thermal stresses. These temperature-induced alterations in the epigenome, particularly their impact on chromatin structural organization, represent an understudied area that could offer...
Source: European Journal of Cell Biology - November 28, 2023 Category: Cytology Authors: Claudio Casali Luca Galgano Lorena Zannino Stella Siciliani Margherita Cavallo Giuliano Mazzini Marco Biggiogera Source Type: research
Impact of heat and cold shock on epigenetics and chromatin structure
In this study, we investigated the epigenetic profiles of human cells subjected to hyperthermia and hypothermia, revealing significant variations. Heat shock primarily led to DNA methylation increments and epigenetic modifications associated with gene expression silencing. In contrast, cold shock presented a complex scenario, with both methylation and demethylation levels increasing, indicating different epigenetic responses to the opposite thermal stresses. These temperature-induced alterations in the epigenome, particularly their impact on chromatin structural organization, represent an understudied area that could offer...
Source: European Journal of Cell Biology - November 28, 2023 Category: Cytology Authors: Claudio Casali Luca Galgano Lorena Zannino Stella Siciliani Margherita Cavallo Giuliano Mazzini Marco Biggiogera Source Type: research
Impact of heat and cold shock on epigenetics and chromatin structure
In this study, we investigated the epigenetic profiles of human cells subjected to hyperthermia and hypothermia, revealing significant variations. Heat shock primarily led to DNA methylation increments and epigenetic modifications associated with gene expression silencing. In contrast, cold shock presented a complex scenario, with both methylation and demethylation levels increasing, indicating different epigenetic responses to the opposite thermal stresses. These temperature-induced alterations in the epigenome, particularly their impact on chromatin structural organization, represent an understudied area that could offer...
Source: European Journal of Cell Biology - November 28, 2023 Category: Cytology Authors: Claudio Casali Luca Galgano Lorena Zannino Stella Siciliani Margherita Cavallo Giuliano Mazzini Marco Biggiogera Source Type: research
Impact of heat and cold shock on epigenetics and chromatin structure
In this study, we investigated the epigenetic profiles of human cells subjected to hyperthermia and hypothermia, revealing significant variations. Heat shock primarily led to DNA methylation increments and epigenetic modifications associated with gene expression silencing. In contrast, cold shock presented a complex scenario, with both methylation and demethylation levels increasing, indicating different epigenetic responses to the opposite thermal stresses. These temperature-induced alterations in the epigenome, particularly their impact on chromatin structural organization, represent an understudied area that could offer...
Source: European Journal of Cell Biology - November 28, 2023 Category: Cytology Authors: Claudio Casali Luca Galgano Lorena Zannino Stella Siciliani Margherita Cavallo Giuliano Mazzini Marco Biggiogera Source Type: research
Impact of heat and cold shock on epigenetics and chromatin structure
In this study, we investigated the epigenetic profiles of human cells subjected to hyperthermia and hypothermia, revealing significant variations. Heat shock primarily led to DNA methylation increments and epigenetic modifications associated with gene expression silencing. In contrast, cold shock presented a complex scenario, with both methylation and demethylation levels increasing, indicating different epigenetic responses to the opposite thermal stresses. These temperature-induced alterations in the epigenome, particularly their impact on chromatin structural organization, represent an understudied area that could offer...
Source: European Journal of Cell Biology - November 28, 2023 Category: Cytology Authors: Claudio Casali Luca Galgano Lorena Zannino Stella Siciliani Margherita Cavallo Giuliano Mazzini Marco Biggiogera Source Type: research
