The solute carrier SLC7A1 may act as a protein transporter at the blood-brain barrier
Eur J Cell Biol. 2024 Mar 21;103(2):151406. doi: 10.1016/j.ejcb.2024.151406. Online ahead of print.ABSTRACTDespite extensive research, targeted delivery of substances to the brain still poses a great challenge due to the selectivity of the blood-brain barrier (BBB). Most molecules require either carrier- or receptor-mediated transport systems to reach the central nervous system (CNS). These transport systems form attractive routes for the delivery of therapeutics into the CNS, yet the number of known brain endothelium-enriched receptors allowing the transport of large molecules into the brain is scarce. Therefore, to ident...
Source: European Journal of Cell Biology - March 28, 2024 Category: Cytology Authors: Magdalena Kurtyka Frank Wessely Sarah Bau Eseoghene Ifie Liqun He Nienke M de Wit Alberte Bay Villekj ær Pedersen Maximilian Keller Caleb Webber Helga E de Vries Olaf Ansorge Christer Betsholtz Marijke De Bock Catarina Chaves Birger Brodin Morten S Niels Source Type: research
The solute carrier SLC7A1 may act as a protein transporter at the blood-brain barrier
Eur J Cell Biol. 2024 Mar 21;103(2):151406. doi: 10.1016/j.ejcb.2024.151406. Online ahead of print.ABSTRACTDespite extensive research, targeted delivery of substances to the brain still poses a great challenge due to the selectivity of the blood-brain barrier (BBB). Most molecules require either carrier- or receptor-mediated transport systems to reach the central nervous system (CNS). These transport systems form attractive routes for the delivery of therapeutics into the CNS, yet the number of known brain endothelium-enriched receptors allowing the transport of large molecules into the brain is scarce. Therefore, to ident...
Source: European Journal of Cell Biology - March 28, 2024 Category: Cytology Authors: Magdalena Kurtyka Frank Wessely Sarah Bau Eseoghene Ifie Liqun He Nienke M de Wit Alberte Bay Villekj ær Pedersen Maximilian Keller Caleb Webber Helga E de Vries Olaf Ansorge Christer Betsholtz Marijke De Bock Catarina Chaves Birger Brodin Morten S Niels Source Type: research
Reconstitution of cytolinker-mediated crosstalk between actin and vimentin
Eur J Cell Biol. 2024 Mar 12;103(2):151403. doi: 10.1016/j.ejcb.2024.151403. Online ahead of print.ABSTRACTCell shape and motility are determined by the cytoskeleton, an interpenetrating network of actin filaments, microtubules, and intermediate filaments. The biophysical properties of each filament type individually have been studied extensively by cell-free reconstitution. By contrast, the interactions between the three cytoskeletal networks are relatively unexplored. They are coupled via crosslinkers of the plakin family such as plectin. These are challenging proteins for reconstitution because of their giant size and m...
Source: European Journal of Cell Biology - March 19, 2024 Category: Cytology Authors: Irene Ist úriz Petitjean Quang D Tran Angeliki Goutou Zima Kabir Gerhard Wiche C écile Leduc Gijsje H Koenderink Source Type: research
The small yeast GTPase Rho5 requires specific mitochondrial outer membrane proteins for translocation under oxidative stress and interacts with the VDAC Por1
Eur J Cell Biol. 2024 Mar 15;103(2):151405. doi: 10.1016/j.ejcb.2024.151405. Online ahead of print.ABSTRACTYeast Rho5 is a small GTPase which mediates the response to nutrient and oxidative stress, and triggers mitophagy and apoptosis. We here studied the rapid translocation of a GFP-tagged Rho5 to mitochondria under such stress conditions by live-cell fluorescence microscopy in the background of strains lacking different mitochondrial outer membrane proteins (MOMP). Fun14, Msp1 and Alo1 were found to be required for efficient recruitment of the GTPase, whereas translocation of Dck1 and Lmo1, the subunits of its dimeric GD...
Source: European Journal of Cell Biology - March 19, 2024 Category: Cytology Authors: Linnet Bischof Franziska Schweitzer Hans-Peter Schmitz J ürgen J Heinisch Source Type: research
Reconstitution of cytolinker-mediated crosstalk between actin and vimentin
Eur J Cell Biol. 2024 Mar 12;103(2):151403. doi: 10.1016/j.ejcb.2024.151403. Online ahead of print.ABSTRACTCell shape and motility are determined by the cytoskeleton, an interpenetrating network of actin filaments, microtubules, and intermediate filaments. The biophysical properties of each filament type individually have been studied extensively by cell-free reconstitution. By contrast, the interactions between the three cytoskeletal networks are relatively unexplored. They are coupled via crosslinkers of the plakin family such as plectin. These are challenging proteins for reconstitution because of their giant size and m...
Source: European Journal of Cell Biology - March 19, 2024 Category: Cytology Authors: Irene Ist úriz Petitjean Quang D Tran Angeliki Goutou Zima Kabir Gerhard Wiche C écile Leduc Gijsje H Koenderink Source Type: research
The small yeast GTPase Rho5 requires specific mitochondrial outer membrane proteins for translocation under oxidative stress and interacts with the VDAC Por1
Eur J Cell Biol. 2024 Mar 15;103(2):151405. doi: 10.1016/j.ejcb.2024.151405. Online ahead of print.ABSTRACTYeast Rho5 is a small GTPase which mediates the response to nutrient and oxidative stress, and triggers mitophagy and apoptosis. We here studied the rapid translocation of a GFP-tagged Rho5 to mitochondria under such stress conditions by live-cell fluorescence microscopy in the background of strains lacking different mitochondrial outer membrane proteins (MOMP). Fun14, Msp1 and Alo1 were found to be required for efficient recruitment of the GTPase, whereas translocation of Dck1 and Lmo1, the subunits of its dimeric GD...
Source: European Journal of Cell Biology - March 19, 2024 Category: Cytology Authors: Linnet Bischof Franziska Schweitzer Hans-Peter Schmitz J ürgen J Heinisch Source Type: research
Reconstitution of cytolinker-mediated crosstalk between actin and vimentin
Eur J Cell Biol. 2024 Mar 12;103(2):151403. doi: 10.1016/j.ejcb.2024.151403. Online ahead of print.ABSTRACTCell shape and motility are determined by the cytoskeleton, an interpenetrating network of actin filaments, microtubules, and intermediate filaments. The biophysical properties of each filament type individually have been studied extensively by cell-free reconstitution. By contrast, the interactions between the three cytoskeletal networks are relatively unexplored. They are coupled via crosslinkers of the plakin family such as plectin. These are challenging proteins for reconstitution because of their giant size and m...
Source: European Journal of Cell Biology - March 19, 2024 Category: Cytology Authors: Irene Ist úriz Petitjean Quang D Tran Angeliki Goutou Zima Kabir Gerhard Wiche C écile Leduc Gijsje H Koenderink Source Type: research
The small yeast GTPase Rho5 requires specific mitochondrial outer membrane proteins for translocation under oxidative stress and interacts with the VDAC Por1
Eur J Cell Biol. 2024 Mar 15;103(2):151405. doi: 10.1016/j.ejcb.2024.151405. Online ahead of print.ABSTRACTYeast Rho5 is a small GTPase which mediates the response to nutrient and oxidative stress, and triggers mitophagy and apoptosis. We here studied the rapid translocation of a GFP-tagged Rho5 to mitochondria under such stress conditions by live-cell fluorescence microscopy in the background of strains lacking different mitochondrial outer membrane proteins (MOMP). Fun14, Msp1 and Alo1 were found to be required for efficient recruitment of the GTPase, whereas translocation of Dck1 and Lmo1, the subunits of its dimeric GD...
Source: European Journal of Cell Biology - March 19, 2024 Category: Cytology Authors: Linnet Bischof Franziska Schweitzer Hans-Peter Schmitz J ürgen J Heinisch Source Type: research
Reconstitution of cytolinker-mediated crosstalk between actin and vimentin
Eur J Cell Biol. 2024 Mar 12;103(2):151403. doi: 10.1016/j.ejcb.2024.151403. Online ahead of print.ABSTRACTCell shape and motility are determined by the cytoskeleton, an interpenetrating network of actin filaments, microtubules, and intermediate filaments. The biophysical properties of each filament type individually have been studied extensively by cell-free reconstitution. By contrast, the interactions between the three cytoskeletal networks are relatively unexplored. They are coupled via crosslinkers of the plakin family such as plectin. These are challenging proteins for reconstitution because of their giant size and m...
Source: European Journal of Cell Biology - March 19, 2024 Category: Cytology Authors: Irene Ist úriz Petitjean Quang D Tran Angeliki Goutou Zima Kabir Gerhard Wiche C écile Leduc Gijsje H Koenderink Source Type: research
The small yeast GTPase Rho5 requires specific mitochondrial outer membrane proteins for translocation under oxidative stress and interacts with the VDAC Por1
Eur J Cell Biol. 2024 Mar 15;103(2):151405. doi: 10.1016/j.ejcb.2024.151405. Online ahead of print.ABSTRACTYeast Rho5 is a small GTPase which mediates the response to nutrient and oxidative stress, and triggers mitophagy and apoptosis. We here studied the rapid translocation of a GFP-tagged Rho5 to mitochondria under such stress conditions by live-cell fluorescence microscopy in the background of strains lacking different mitochondrial outer membrane proteins (MOMP). Fun14, Msp1 and Alo1 were found to be required for efficient recruitment of the GTPase, whereas translocation of Dck1 and Lmo1, the subunits of its dimeric GD...
Source: European Journal of Cell Biology - March 19, 2024 Category: Cytology Authors: Linnet Bischof Franziska Schweitzer Hans-Peter Schmitz J ürgen J Heinisch Source Type: research
Reconstitution of cytolinker-mediated crosstalk between actin and vimentin
Eur J Cell Biol. 2024 Mar 12;103(2):151403. doi: 10.1016/j.ejcb.2024.151403. Online ahead of print.ABSTRACTCell shape and motility are determined by the cytoskeleton, an interpenetrating network of actin filaments, microtubules, and intermediate filaments. The biophysical properties of each filament type individually have been studied extensively by cell-free reconstitution. By contrast, the interactions between the three cytoskeletal networks are relatively unexplored. They are coupled via crosslinkers of the plakin family such as plectin. These are challenging proteins for reconstitution because of their giant size and m...
Source: European Journal of Cell Biology - March 19, 2024 Category: Cytology Authors: Irene Ist úriz Petitjean Quang D Tran Angeliki Goutou Zima Kabir Gerhard Wiche C écile Leduc Gijsje H Koenderink Source Type: research
The small yeast GTPase Rho5 requires specific mitochondrial outer membrane proteins for translocation under oxidative stress and interacts with the VDAC Por1
Eur J Cell Biol. 2024 Mar 15;103(2):151405. doi: 10.1016/j.ejcb.2024.151405. Online ahead of print.ABSTRACTYeast Rho5 is a small GTPase which mediates the response to nutrient and oxidative stress, and triggers mitophagy and apoptosis. We here studied the rapid translocation of a GFP-tagged Rho5 to mitochondria under such stress conditions by live-cell fluorescence microscopy in the background of strains lacking different mitochondrial outer membrane proteins (MOMP). Fun14, Msp1 and Alo1 were found to be required for efficient recruitment of the GTPase, whereas translocation of Dck1 and Lmo1, the subunits of its dimeric GD...
Source: European Journal of Cell Biology - March 19, 2024 Category: Cytology Authors: Linnet Bischof Franziska Schweitzer Hans-Peter Schmitz J ürgen J Heinisch Source Type: research
Reconstitution of cytolinker-mediated crosstalk between actin and vimentin
Eur J Cell Biol. 2024 Mar 12;103(2):151403. doi: 10.1016/j.ejcb.2024.151403. Online ahead of print.ABSTRACTCell shape and motility are determined by the cytoskeleton, an interpenetrating network of actin filaments, microtubules, and intermediate filaments. The biophysical properties of each filament type individually have been studied extensively by cell-free reconstitution. By contrast, the interactions between the three cytoskeletal networks are relatively unexplored. They are coupled via crosslinkers of the plakin family such as plectin. These are challenging proteins for reconstitution because of their giant size and m...
Source: European Journal of Cell Biology - March 19, 2024 Category: Cytology Authors: Irene Ist úriz Petitjean Quang D Tran Angeliki Goutou Zima Kabir Gerhard Wiche C écile Leduc Gijsje H Koenderink Source Type: research
The small yeast GTPase Rho5 requires specific mitochondrial outer membrane proteins for translocation under oxidative stress and interacts with the VDAC Por1
Eur J Cell Biol. 2024 Mar 15;103(2):151405. doi: 10.1016/j.ejcb.2024.151405. Online ahead of print.ABSTRACTYeast Rho5 is a small GTPase which mediates the response to nutrient and oxidative stress, and triggers mitophagy and apoptosis. We here studied the rapid translocation of a GFP-tagged Rho5 to mitochondria under such stress conditions by live-cell fluorescence microscopy in the background of strains lacking different mitochondrial outer membrane proteins (MOMP). Fun14, Msp1 and Alo1 were found to be required for efficient recruitment of the GTPase, whereas translocation of Dck1 and Lmo1, the subunits of its dimeric GD...
Source: European Journal of Cell Biology - March 19, 2024 Category: Cytology Authors: Linnet Bischof Franziska Schweitzer Hans-Peter Schmitz J ürgen J Heinisch Source Type: research
Reconstitution of cytolinker-mediated crosstalk between actin and vimentin
Eur J Cell Biol. 2024 Mar 12;103(2):151403. doi: 10.1016/j.ejcb.2024.151403. Online ahead of print.ABSTRACTCell shape and motility are determined by the cytoskeleton, an interpenetrating network of actin filaments, microtubules, and intermediate filaments. The biophysical properties of each filament type individually have been studied extensively by cell-free reconstitution. By contrast, the interactions between the three cytoskeletal networks are relatively unexplored. They are coupled via crosslinkers of the plakin family such as plectin. These are challenging proteins for reconstitution because of their giant size and m...
Source: European Journal of Cell Biology - March 19, 2024 Category: Cytology Authors: Irene Ist úriz Petitjean Quang D Tran Angeliki Goutou Zima Kabir Gerhard Wiche C écile Leduc Gijsje H Koenderink Source Type: research
