The small yeast GTPase Rho5 requires specific mitochondrial outer membrane proteins for translocation under oxidative stress and interacts with the VDAC Por1
Eur J Cell Biol. 2024 Mar 15;103(2):151405. doi: 10.1016/j.ejcb.2024.151405. Online ahead of print.ABSTRACTYeast Rho5 is a small GTPase which mediates the response to nutrient and oxidative stress, and triggers mitophagy and apoptosis. We here studied the rapid translocation of a GFP-tagged Rho5 to mitochondria under such stress conditions by live-cell fluorescence microscopy in the background of strains lacking different mitochondrial outer membrane proteins (MOMP). Fun14, Msp1 and Alo1 were found to be required for efficient recruitment of the GTPase, whereas translocation of Dck1 and Lmo1, the subunits of its dimeric GD...
Source: European Journal of Cell Biology - March 19, 2024 Category: Cytology Authors: Linnet Bischof Franziska Schweitzer Hans-Peter Schmitz J ürgen J Heinisch Source Type: research
Reconstitution of cytolinker-mediated crosstalk between actin and vimentin
Eur J Cell Biol. 2024 Mar 12;103(2):151403. doi: 10.1016/j.ejcb.2024.151403. Online ahead of print.ABSTRACTCell shape and motility are determined by the cytoskeleton, an interpenetrating network of actin filaments, microtubules, and intermediate filaments. The biophysical properties of each filament type individually have been studied extensively by cell-free reconstitution. By contrast, the interactions between the three cytoskeletal networks are relatively unexplored. They are coupled via crosslinkers of the plakin family such as plectin. These are challenging proteins for reconstitution because of their giant size and m...
Source: European Journal of Cell Biology - March 19, 2024 Category: Cytology Authors: Irene Ist úriz Petitjean Quang D Tran Angeliki Goutou Zima Kabir Gerhard Wiche C écile Leduc Gijsje H Koenderink Source Type: research
The small yeast GTPase Rho5 requires specific mitochondrial outer membrane proteins for translocation under oxidative stress and interacts with the VDAC Por1
Eur J Cell Biol. 2024 Mar 15;103(2):151405. doi: 10.1016/j.ejcb.2024.151405. Online ahead of print.ABSTRACTYeast Rho5 is a small GTPase which mediates the response to nutrient and oxidative stress, and triggers mitophagy and apoptosis. We here studied the rapid translocation of a GFP-tagged Rho5 to mitochondria under such stress conditions by live-cell fluorescence microscopy in the background of strains lacking different mitochondrial outer membrane proteins (MOMP). Fun14, Msp1 and Alo1 were found to be required for efficient recruitment of the GTPase, whereas translocation of Dck1 and Lmo1, the subunits of its dimeric GD...
Source: European Journal of Cell Biology - March 19, 2024 Category: Cytology Authors: Linnet Bischof Franziska Schweitzer Hans-Peter Schmitz J ürgen J Heinisch Source Type: research
Arp2/3 complex- and formin-mediated actin cytoskeleton networks facilitate actin binding protein sorting in fission yeast
We report that actin assembly factors Arp2/3 complex and formin Cdc12 tune the association of ABPs fimbrin Fim1 and tropomyosin Cdc8 to different F-actin networks in fission yeast. Genetic and pharmacological disruption of F-actin networks revealed that Fim1 is preferentially directed to Arp2/3-complex mediated actin patches, whereas Cdc8 is preferentially targeted to formin Cdc12-mediated filaments in the contractile ring. To investigate the role of Arp2/3 complex- and formin Cdc12-mediated actin assembly, we used four-color TIRF microscopy to observe the in vitro reconstitution of ABP sorting with purified proteins. Fim1...
Source: European Journal of Cell Biology - March 17, 2024 Category: Cytology Authors: Kaitlin E Homa Glen M Hocky Cristian Suarez David R Kovar Source Type: research
Arp2/3 complex- and formin-mediated actin cytoskeleton networks facilitate actin binding protein sorting in fission yeast
We report that actin assembly factors Arp2/3 complex and formin Cdc12 tune the association of ABPs fimbrin Fim1 and tropomyosin Cdc8 to different F-actin networks in fission yeast. Genetic and pharmacological disruption of F-actin networks revealed that Fim1 is preferentially directed to Arp2/3-complex mediated actin patches, whereas Cdc8 is preferentially targeted to formin Cdc12-mediated filaments in the contractile ring. To investigate the role of Arp2/3 complex- and formin Cdc12-mediated actin assembly, we used four-color TIRF microscopy to observe the in vitro reconstitution of ABP sorting with purified proteins. Fim1...
Source: European Journal of Cell Biology - March 17, 2024 Category: Cytology Authors: Kaitlin E Homa Glen M Hocky Cristian Suarez David R Kovar Source Type: research
Actin-membrane linkers: Insights from synthetic reconstituted systems
Eur J Cell Biol. 2024 Mar 1;103(2):151402. doi: 10.1016/j.ejcb.2024.151402. Online ahead of print.ABSTRACTAt the cell surface, the actin cytoskeleton and the plasma membrane interact reciprocally in a variety of processes related to the remodeling of the cell surface. The actin cytoskeleton has been known to modulate membrane organization and reshape the membrane. To this end, actin-membrane linking molecules play a major role in regulating actin assembly and spatially direct the interaction between the actin cytoskeleton and the membrane. While studies in cells have provided a wealth of knowledge on the molecular composit...
Source: European Journal of Cell Biology - March 10, 2024 Category: Cytology Authors: Feng-Ching Tsai Gwendal Gu érin Julien Pernier Patricia Bassereau Source Type: research
Actin-membrane linkers: Insights from synthetic reconstituted systems
Eur J Cell Biol. 2024 Mar 1;103(2):151402. doi: 10.1016/j.ejcb.2024.151402. Online ahead of print.ABSTRACTAt the cell surface, the actin cytoskeleton and the plasma membrane interact reciprocally in a variety of processes related to the remodeling of the cell surface. The actin cytoskeleton has been known to modulate membrane organization and reshape the membrane. To this end, actin-membrane linking molecules play a major role in regulating actin assembly and spatially direct the interaction between the actin cytoskeleton and the membrane. While studies in cells have provided a wealth of knowledge on the molecular composit...
Source: European Journal of Cell Biology - March 10, 2024 Category: Cytology Authors: Feng-Ching Tsai Gwendal Gu érin Julien Pernier Patricia Bassereau Source Type: research
Actin-membrane linkers: Insights from synthetic reconstituted systems
Eur J Cell Biol. 2024 Mar 1;103(2):151402. doi: 10.1016/j.ejcb.2024.151402. Online ahead of print.ABSTRACTAt the cell surface, the actin cytoskeleton and the plasma membrane interact reciprocally in a variety of processes related to the remodeling of the cell surface. The actin cytoskeleton has been known to modulate membrane organization and reshape the membrane. To this end, actin-membrane linking molecules play a major role in regulating actin assembly and spatially direct the interaction between the actin cytoskeleton and the membrane. While studies in cells have provided a wealth of knowledge on the molecular composit...
Source: European Journal of Cell Biology - March 10, 2024 Category: Cytology Authors: Feng-Ching Tsai Gwendal Gu érin Julien Pernier Patricia Bassereau Source Type: research
Actin-membrane linkers: Insights from synthetic reconstituted systems
Eur J Cell Biol. 2024 Mar 1;103(2):151402. doi: 10.1016/j.ejcb.2024.151402. Online ahead of print.ABSTRACTAt the cell surface, the actin cytoskeleton and the plasma membrane interact reciprocally in a variety of processes related to the remodeling of the cell surface. The actin cytoskeleton has been known to modulate membrane organization and reshape the membrane. To this end, actin-membrane linking molecules play a major role in regulating actin assembly and spatially direct the interaction between the actin cytoskeleton and the membrane. While studies in cells have provided a wealth of knowledge on the molecular composit...
Source: European Journal of Cell Biology - March 10, 2024 Category: Cytology Authors: Feng-Ching Tsai Gwendal Gu érin Julien Pernier Patricia Bassereau Source Type: research
Actin-membrane linkers: Insights from synthetic reconstituted systems
Eur J Cell Biol. 2024 Mar 1;103(2):151402. doi: 10.1016/j.ejcb.2024.151402. Online ahead of print.ABSTRACTAt the cell surface, the actin cytoskeleton and the plasma membrane interact reciprocally in a variety of processes related to the remodeling of the cell surface. The actin cytoskeleton has been known to modulate membrane organization and reshape the membrane. To this end, actin-membrane linking molecules play a major role in regulating actin assembly and spatially direct the interaction between the actin cytoskeleton and the membrane. While studies in cells have provided a wealth of knowledge on the molecular composit...
Source: European Journal of Cell Biology - March 10, 2024 Category: Cytology Authors: Feng-Ching Tsai Gwendal Gu érin Julien Pernier Patricia Bassereau Source Type: research
Actin-membrane linkers: Insights from synthetic reconstituted systems
Eur J Cell Biol. 2024 Mar 1;103(2):151402. doi: 10.1016/j.ejcb.2024.151402. Online ahead of print.ABSTRACTAt the cell surface, the actin cytoskeleton and the plasma membrane interact reciprocally in a variety of processes related to the remodeling of the cell surface. The actin cytoskeleton has been known to modulate membrane organization and reshape the membrane. To this end, actin-membrane linking molecules play a major role in regulating actin assembly and spatially direct the interaction between the actin cytoskeleton and the membrane. While studies in cells have provided a wealth of knowledge on the molecular composit...
Source: European Journal of Cell Biology - March 10, 2024 Category: Cytology Authors: Feng-Ching Tsai Gwendal Gu érin Julien Pernier Patricia Bassereau Source Type: research
Cross-regulation of Listeria monocytogenes and the host ubiquitin system in listeriosis
Eur J Cell Biol. 2024 Feb 28;103(2):151401. doi: 10.1016/j.ejcb.2024.151401. Online ahead of print.ABSTRACTThe facultative intracellular bacterium Listeria (L.) monocytogenes may cause severe diseases in humans and animals. The control of listeriosis/L. monocytogenes requires the concerted action of cells of the innate and adaptive immune systems. In this regard, cell-intrinsic immunity of infected cells, activated by the immune responses, is crucial for the control and elimination intracellular L. monocytogenes. Both the immune response against L. monocytogenes and cell intrinsic pathogen control are critically regulated ...
Source: European Journal of Cell Biology - March 5, 2024 Category: Cytology Authors: Yuan Zhuang Johanna B Fischer Gopala Nishanth Dirk Schl üter Source Type: research
Cross-regulation of Listeria monocytogenes and the host ubiquitin system in listeriosis
Eur J Cell Biol. 2024 Feb 28;103(2):151401. doi: 10.1016/j.ejcb.2024.151401. Online ahead of print.ABSTRACTThe facultative intracellular bacterium Listeria (L.) monocytogenes may cause severe diseases in humans and animals. The control of listeriosis/L. monocytogenes requires the concerted action of cells of the innate and adaptive immune systems. In this regard, cell-intrinsic immunity of infected cells, activated by the immune responses, is crucial for the control and elimination intracellular L. monocytogenes. Both the immune response against L. monocytogenes and cell intrinsic pathogen control are critically regulated ...
Source: European Journal of Cell Biology - March 5, 2024 Category: Cytology Authors: Yuan Zhuang Johanna B Fischer Gopala Nishanth Dirk Schl üter Source Type: research
Cross-regulation of Listeria monocytogenes and the host ubiquitin system in listeriosis
Eur J Cell Biol. 2024 Feb 28;103(2):151401. doi: 10.1016/j.ejcb.2024.151401. Online ahead of print.ABSTRACTThe facultative intracellular bacterium Listeria (L.) monocytogenes may cause severe diseases in humans and animals. The control of listeriosis/L. monocytogenes requires the concerted action of cells of the innate and adaptive immune systems. In this regard, cell-intrinsic immunity of infected cells, activated by the immune responses, is crucial for the control and elimination intracellular L. monocytogenes. Both the immune response against L. monocytogenes and cell intrinsic pathogen control are critically regulated ...
Source: European Journal of Cell Biology - March 5, 2024 Category: Cytology Authors: Yuan Zhuang Johanna B Fischer Gopala Nishanth Dirk Schl üter Source Type: research
Cross-regulation of Listeria monocytogenes and the host ubiquitin system in listeriosis
Eur J Cell Biol. 2024 Feb 28;103(2):151401. doi: 10.1016/j.ejcb.2024.151401. Online ahead of print.ABSTRACTThe facultative intracellular bacterium Listeria (L.) monocytogenes may cause severe diseases in humans and animals. The control of listeriosis/L. monocytogenes requires the concerted action of cells of the innate and adaptive immune systems. In this regard, cell-intrinsic immunity of infected cells, activated by the immune responses, is crucial for the control and elimination intracellular L. monocytogenes. Both the immune response against L. monocytogenes and cell intrinsic pathogen control are critically regulated ...
Source: European Journal of Cell Biology - March 5, 2024 Category: Cytology Authors: Yuan Zhuang Johanna B Fischer Gopala Nishanth Dirk Schl üter Source Type: research
