Loss of mfsd8 alters the secretome during Dictyostelium aggregation
Eur J Cell Biol. 2023 Sep 19;102(4):151361. doi: 10.1016/j.ejcb.2023.151361. Online ahead of print.ABSTRACTMajor facilitator superfamily domain-containing protein 8 (MFSD8) is a transmembrane protein that has been reported to function as a lysosomal chloride channel. In humans, homozygous mutations in MFSD8 cause a late-infantile form of neuronal ceroid lipofuscinosis (NCL) called CLN7 disease. In the social amoeba Dictyostelium discoideum, Mfsd8 localizes to cytoplasmic puncta and vesicles, and regulates conserved processes during the organism's life cycle. Here, we used D. discoideum to examine the effect of mfsd8-defici...
Source: European Journal of Cell Biology - September 24, 2023 Category: Cytology Authors: Robert J Huber Joshua Gray William D Kim Source Type: research
Concept of lipid droplet biogenesis
Eur J Cell Biol. 2023 Sep 19;102(4):151362. doi: 10.1016/j.ejcb.2023.151362. Online ahead of print.ABSTRACTLipid droplets (LD) are functionally conserved fat storage organelles found in all cell types. LDs have a unique structure comprising of a hydrophobic core of neutral lipids (fat), triacylglycerol (TAG) and cholesterol esters (CE) surrounded by a phospholipid monolayer. LD surface is decorated by a multitude of proteins and enzymes rendering this compartment functional. Accumulating evidence suggests that LDs originate from discrete ER-subdomains, demarcated by the lipodystrophy protein seipin, however, the mechanisms...
Source: European Journal of Cell Biology - September 24, 2023 Category: Cytology Authors: R Mankamna Kumari Amit Khatri Ritika Chaudhary Vineet Choudhary Source Type: research
Loss of mfsd8 alters the secretome during Dictyostelium aggregation
Eur J Cell Biol. 2023 Sep 19;102(4):151361. doi: 10.1016/j.ejcb.2023.151361. Online ahead of print.ABSTRACTMajor facilitator superfamily domain-containing protein 8 (MFSD8) is a transmembrane protein that has been reported to function as a lysosomal chloride channel. In humans, homozygous mutations in MFSD8 cause a late-infantile form of neuronal ceroid lipofuscinosis (NCL) called CLN7 disease. In the social amoeba Dictyostelium discoideum, Mfsd8 localizes to cytoplasmic puncta and vesicles, and regulates conserved processes during the organism's life cycle. Here, we used D. discoideum to examine the effect of mfsd8-defici...
Source: European Journal of Cell Biology - September 24, 2023 Category: Cytology Authors: Robert J Huber Joshua Gray William D Kim Source Type: research
Concept of lipid droplet biogenesis
Eur J Cell Biol. 2023 Sep 19;102(4):151362. doi: 10.1016/j.ejcb.2023.151362. Online ahead of print.ABSTRACTLipid droplets (LD) are functionally conserved fat storage organelles found in all cell types. LDs have a unique structure comprising of a hydrophobic core of neutral lipids (fat), triacylglycerol (TAG) and cholesterol esters (CE) surrounded by a phospholipid monolayer. LD surface is decorated by a multitude of proteins and enzymes rendering this compartment functional. Accumulating evidence suggests that LDs originate from discrete ER-subdomains, demarcated by the lipodystrophy protein seipin, however, the mechanisms...
Source: European Journal of Cell Biology - September 24, 2023 Category: Cytology Authors: R Mankamna Kumari Amit Khatri Ritika Chaudhary Vineet Choudhary Source Type: research
Loss of mfsd8 alters the secretome during Dictyostelium aggregation
Eur J Cell Biol. 2023 Sep 19;102(4):151361. doi: 10.1016/j.ejcb.2023.151361. Online ahead of print.ABSTRACTMajor facilitator superfamily domain-containing protein 8 (MFSD8) is a transmembrane protein that has been reported to function as a lysosomal chloride channel. In humans, homozygous mutations in MFSD8 cause a late-infantile form of neuronal ceroid lipofuscinosis (NCL) called CLN7 disease. In the social amoeba Dictyostelium discoideum, Mfsd8 localizes to cytoplasmic puncta and vesicles, and regulates conserved processes during the organism's life cycle. Here, we used D. discoideum to examine the effect of mfsd8-defici...
Source: European Journal of Cell Biology - September 24, 2023 Category: Cytology Authors: Robert J Huber Joshua Gray William D Kim Source Type: research
Concept of lipid droplet biogenesis
Eur J Cell Biol. 2023 Sep 19;102(4):151362. doi: 10.1016/j.ejcb.2023.151362. Online ahead of print.ABSTRACTLipid droplets (LD) are functionally conserved fat storage organelles found in all cell types. LDs have a unique structure comprising of a hydrophobic core of neutral lipids (fat), triacylglycerol (TAG) and cholesterol esters (CE) surrounded by a phospholipid monolayer. LD surface is decorated by a multitude of proteins and enzymes rendering this compartment functional. Accumulating evidence suggests that LDs originate from discrete ER-subdomains, demarcated by the lipodystrophy protein seipin, however, the mechanisms...
Source: European Journal of Cell Biology - September 24, 2023 Category: Cytology Authors: R Mankamna Kumari Amit Khatri Ritika Chaudhary Vineet Choudhary Source Type: research
Loss of mfsd8 alters the secretome during Dictyostelium aggregation
Eur J Cell Biol. 2023 Sep 19;102(4):151361. doi: 10.1016/j.ejcb.2023.151361. Online ahead of print.ABSTRACTMajor facilitator superfamily domain-containing protein 8 (MFSD8) is a transmembrane protein that has been reported to function as a lysosomal chloride channel. In humans, homozygous mutations in MFSD8 cause a late-infantile form of neuronal ceroid lipofuscinosis (NCL) called CLN7 disease. In the social amoeba Dictyostelium discoideum, Mfsd8 localizes to cytoplasmic puncta and vesicles, and regulates conserved processes during the organism's life cycle. Here, we used D. discoideum to examine the effect of mfsd8-defici...
Source: European Journal of Cell Biology - September 24, 2023 Category: Cytology Authors: Robert J Huber Joshua Gray William D Kim Source Type: research
Concept of lipid droplet biogenesis
Eur J Cell Biol. 2023 Sep 19;102(4):151362. doi: 10.1016/j.ejcb.2023.151362. Online ahead of print.ABSTRACTLipid droplets (LD) are functionally conserved fat storage organelles found in all cell types. LDs have a unique structure comprising of a hydrophobic core of neutral lipids (fat), triacylglycerol (TAG) and cholesterol esters (CE) surrounded by a phospholipid monolayer. LD surface is decorated by a multitude of proteins and enzymes rendering this compartment functional. Accumulating evidence suggests that LDs originate from discrete ER-subdomains, demarcated by the lipodystrophy protein seipin, however, the mechanisms...
Source: European Journal of Cell Biology - September 24, 2023 Category: Cytology Authors: R Mankamna Kumari Amit Khatri Ritika Chaudhary Vineet Choudhary Source Type: research
Loss of mfsd8 alters the secretome during Dictyostelium aggregation
Eur J Cell Biol. 2023 Sep 19;102(4):151361. doi: 10.1016/j.ejcb.2023.151361. Online ahead of print.ABSTRACTMajor facilitator superfamily domain-containing protein 8 (MFSD8) is a transmembrane protein that has been reported to function as a lysosomal chloride channel. In humans, homozygous mutations in MFSD8 cause a late-infantile form of neuronal ceroid lipofuscinosis (NCL) called CLN7 disease. In the social amoeba Dictyostelium discoideum, Mfsd8 localizes to cytoplasmic puncta and vesicles, and regulates conserved processes during the organism's life cycle. Here, we used D. discoideum to examine the effect of mfsd8-defici...
Source: European Journal of Cell Biology - September 24, 2023 Category: Cytology Authors: Robert J Huber Joshua Gray William D Kim Source Type: research
N-glycosylation at N57/100/110 affects CD44s localization, function and stability in hepatocellular carcinoma
Eur J Cell Biol. 2023 Sep 9;102(4):151360. doi: 10.1016/j.ejcb.2023.151360. Online ahead of print.ABSTRACTThe glycosylation levels of proteins in cancer cells are closely related to cancer invasion and migration. CD44 is a transmembrane glycoprotein that is significantly overexpressed in a variety of tumor cells and has been proven to promote the migration and motility of cancer cells, but the effect of its N-glycosylation modification on CD44 protein function in tumors is less studied. Here, we investigated the effect of six N-glycan chains (N25/57/100/110/120/255) on CD44s localization, function and stability in hepatoca...
Source: European Journal of Cell Biology - September 13, 2023 Category: Cytology Authors: Qixiang Cheng Xibo Hu Xiaoqing Zhang Depeng Yang Guiping Zhao Liping Sun Meiyi Jiang Lijun Yang Jialing Cai Bing Wang Mengmeng Zhang Fang Han Yu Li Huan Nie Source Type: research
RhoB promotes Salmonella survival by regulating autophagy
Eur J Cell Biol. 2023 Aug 30;102(4):151358. doi: 10.1016/j.ejcb.2023.151358. Online ahead of print.ABSTRACTSalmonella enterica serovar Typhimurium manipulates cellular Rho GTPases for host cell invasion by effector protein translocation via the Type III Secretion System (T3SS). The two Guanine nucleotide exchange (GEF) mimicking factors SopE and -E2 and the inositol phosphate phosphatase (PiPase) SopB activate the Rho GTPases Rac1, Cdc42 and RhoA, thereby mediating bacterial invasion. S. Typhimurium lacking these three effector proteins are largely invasion-defective. Type III secretion is crucial for both early and later ...
Source: European Journal of Cell Biology - September 13, 2023 Category: Cytology Authors: Marco Kirchenwitz Jessica Halfen Kristin von Peinen Silvia Prettin Jana Kollasser Susanne Zur Lage Wulf Blankenfeldt Cord Brakebusch Klemens Rottner Anika Steffen Theresia E B Stradal Source Type: research
N-glycosylation at N57/100/110 affects CD44s localization, function and stability in hepatocellular carcinoma
Eur J Cell Biol. 2023 Sep 9;102(4):151360. doi: 10.1016/j.ejcb.2023.151360. Online ahead of print.ABSTRACTThe glycosylation levels of proteins in cancer cells are closely related to cancer invasion and migration. CD44 is a transmembrane glycoprotein that is significantly overexpressed in a variety of tumor cells and has been proven to promote the migration and motility of cancer cells, but the effect of its N-glycosylation modification on CD44 protein function in tumors is less studied. Here, we investigated the effect of six N-glycan chains (N25/57/100/110/120/255) on CD44s localization, function and stability in hepatoca...
Source: European Journal of Cell Biology - September 13, 2023 Category: Cytology Authors: Qixiang Cheng Xibo Hu Xiaoqing Zhang Depeng Yang Guiping Zhao Liping Sun Meiyi Jiang Lijun Yang Jialing Cai Bing Wang Mengmeng Zhang Fang Han Yu Li Huan Nie Source Type: research
RhoB promotes Salmonella survival by regulating autophagy
Eur J Cell Biol. 2023 Aug 30;102(4):151358. doi: 10.1016/j.ejcb.2023.151358. Online ahead of print.ABSTRACTSalmonella enterica serovar Typhimurium manipulates cellular Rho GTPases for host cell invasion by effector protein translocation via the Type III Secretion System (T3SS). The two Guanine nucleotide exchange (GEF) mimicking factors SopE and -E2 and the inositol phosphate phosphatase (PiPase) SopB activate the Rho GTPases Rac1, Cdc42 and RhoA, thereby mediating bacterial invasion. S. Typhimurium lacking these three effector proteins are largely invasion-defective. Type III secretion is crucial for both early and later ...
Source: European Journal of Cell Biology - September 13, 2023 Category: Cytology Authors: Marco Kirchenwitz Jessica Halfen Kristin von Peinen Silvia Prettin Jana Kollasser Susanne Zur Lage Wulf Blankenfeldt Cord Brakebusch Klemens Rottner Anika Steffen Theresia E B Stradal Source Type: research
N-glycosylation at N57/100/110 affects CD44s localization, function and stability in hepatocellular carcinoma
Eur J Cell Biol. 2023 Sep 9;102(4):151360. doi: 10.1016/j.ejcb.2023.151360. Online ahead of print.ABSTRACTThe glycosylation levels of proteins in cancer cells are closely related to cancer invasion and migration. CD44 is a transmembrane glycoprotein that is significantly overexpressed in a variety of tumor cells and has been proven to promote the migration and motility of cancer cells, but the effect of its N-glycosylation modification on CD44 protein function in tumors is less studied. Here, we investigated the effect of six N-glycan chains (N25/57/100/110/120/255) on CD44s localization, function and stability in hepatoca...
Source: European Journal of Cell Biology - September 13, 2023 Category: Cytology Authors: Qixiang Cheng Xibo Hu Xiaoqing Zhang Depeng Yang Guiping Zhao Liping Sun Meiyi Jiang Lijun Yang Jialing Cai Bing Wang Mengmeng Zhang Fang Han Yu Li Huan Nie Source Type: research
RhoB promotes Salmonella survival by regulating autophagy
Eur J Cell Biol. 2023 Aug 30;102(4):151358. doi: 10.1016/j.ejcb.2023.151358. Online ahead of print.ABSTRACTSalmonella enterica serovar Typhimurium manipulates cellular Rho GTPases for host cell invasion by effector protein translocation via the Type III Secretion System (T3SS). The two Guanine nucleotide exchange (GEF) mimicking factors SopE and -E2 and the inositol phosphate phosphatase (PiPase) SopB activate the Rho GTPases Rac1, Cdc42 and RhoA, thereby mediating bacterial invasion. S. Typhimurium lacking these three effector proteins are largely invasion-defective. Type III secretion is crucial for both early and later ...
Source: European Journal of Cell Biology - September 13, 2023 Category: Cytology Authors: Marco Kirchenwitz Jessica Halfen Kristin von Peinen Silvia Prettin Jana Kollasser Susanne Zur Lage Wulf Blankenfeldt Cord Brakebusch Klemens Rottner Anika Steffen Theresia E B Stradal Source Type: research
