Impact of heat and cold shock on epigenetics and chromatin structure
In this study, we investigated the epigenetic profiles of human cells subjected to hyperthermia and hypothermia, revealing significant variations. Heat shock primarily led to DNA methylation increments and epigenetic modifications associated with gene expression silencing. In contrast, cold shock presented a complex scenario, with both methylation and demethylation levels increasing, indicating different epigenetic responses to the opposite thermal stresses. These temperature-induced alterations in the epigenome, particularly their impact on chromatin structural organization, represent an understudied area that could offer...
Source: European Journal of Cell Biology - November 28, 2023 Category: Cytology Authors: Claudio Casali Luca Galgano Lorena Zannino Stella Siciliani Margherita Cavallo Giuliano Mazzini Marco Biggiogera Source Type: research
Impact of heat and cold shock on epigenetics and chromatin structure
In this study, we investigated the epigenetic profiles of human cells subjected to hyperthermia and hypothermia, revealing significant variations. Heat shock primarily led to DNA methylation increments and epigenetic modifications associated with gene expression silencing. In contrast, cold shock presented a complex scenario, with both methylation and demethylation levels increasing, indicating different epigenetic responses to the opposite thermal stresses. These temperature-induced alterations in the epigenome, particularly their impact on chromatin structural organization, represent an understudied area that could offer...
Source: European Journal of Cell Biology - November 28, 2023 Category: Cytology Authors: Claudio Casali Luca Galgano Lorena Zannino Stella Siciliani Margherita Cavallo Giuliano Mazzini Marco Biggiogera Source Type: research
Impact of heat and cold shock on epigenetics and chromatin structure
In this study, we investigated the epigenetic profiles of human cells subjected to hyperthermia and hypothermia, revealing significant variations. Heat shock primarily led to DNA methylation increments and epigenetic modifications associated with gene expression silencing. In contrast, cold shock presented a complex scenario, with both methylation and demethylation levels increasing, indicating different epigenetic responses to the opposite thermal stresses. These temperature-induced alterations in the epigenome, particularly their impact on chromatin structural organization, represent an understudied area that could offer...
Source: European Journal of Cell Biology - November 28, 2023 Category: Cytology Authors: Claudio Casali Luca Galgano Lorena Zannino Stella Siciliani Margherita Cavallo Giuliano Mazzini Marco Biggiogera Source Type: research
Impact of heat and cold shock on epigenetics and chromatin structure
In this study, we investigated the epigenetic profiles of human cells subjected to hyperthermia and hypothermia, revealing significant variations. Heat shock primarily led to DNA methylation increments and epigenetic modifications associated with gene expression silencing. In contrast, cold shock presented a complex scenario, with both methylation and demethylation levels increasing, indicating different epigenetic responses to the opposite thermal stresses. These temperature-induced alterations in the epigenome, particularly their impact on chromatin structural organization, represent an understudied area that could offer...
Source: European Journal of Cell Biology - November 28, 2023 Category: Cytology Authors: Claudio Casali Luca Galgano Lorena Zannino Stella Siciliani Margherita Cavallo Giuliano Mazzini Marco Biggiogera Source Type: research
Oxidative stress and signaling through EGFR and PKA pathways converge on the nuclear transport factor RanBP1
Eur J Cell Biol. 2023 Nov 22;103(1):151376. doi: 10.1016/j.ejcb.2023.151376. Online ahead of print.ABSTRACTNuclear protein trafficking requires the soluble transport factor RanBP1. The subcellular distribution of RanBP1 is dynamic, as the protein shuttles between the nucleus and cytoplasm. To date, the signaling pathways regulating RanBP1 subcellular localization are poorly understood. During interphase, RanBP1 resides mostly in the cytoplasm. We show here that oxidative stress concentrates RanBP1 in the nucleus, and our study defines the underlying mechanisms. Specifically, RanBP1's cysteine residues are not essential for...
Source: European Journal of Cell Biology - November 27, 2023 Category: Cytology Authors: Mohamed Kodiha Nabila Azad Siwei Chu Noah Crampton Ursula Stochaj Source Type: research
Oxidative stress and signaling through EGFR and PKA pathways converge on the nuclear transport factor RanBP1
Eur J Cell Biol. 2023 Nov 22;103(1):151376. doi: 10.1016/j.ejcb.2023.151376. Online ahead of print.ABSTRACTNuclear protein trafficking requires the soluble transport factor RanBP1. The subcellular distribution of RanBP1 is dynamic, as the protein shuttles between the nucleus and cytoplasm. To date, the signaling pathways regulating RanBP1 subcellular localization are poorly understood. During interphase, RanBP1 resides mostly in the cytoplasm. We show here that oxidative stress concentrates RanBP1 in the nucleus, and our study defines the underlying mechanisms. Specifically, RanBP1's cysteine residues are not essential for...
Source: European Journal of Cell Biology - November 27, 2023 Category: Cytology Authors: Mohamed Kodiha Nabila Azad Siwei Chu Noah Crampton Ursula Stochaj Source Type: research
Sustained PGC-1 α2 or PGC-1α3 expression induces astrocyte dysfunction and degeneration
Eur J Cell Biol. 2023 Nov 19;103(1):151377. doi: 10.1016/j.ejcb.2023.151377. Online ahead of print.ABSTRACTPeroxisome proliferator-activated receptor-gamma coactivator-1α (PGC-1α) transcriptional coactivators are key regulators of energy metabolism-related genes and are expressed in energy-demanding tissues. There are several PGC-1α variants with different biological functions in different tissues. The brain is one of the tissues where the role of PGC-1α isoforms remains less explored. Here, we used a toxin-based mouse model of Parkinson's disease (PD) and observed that the expression levels of variants PGC-1α2 and PG...
Source: European Journal of Cell Biology - November 25, 2023 Category: Cytology Authors: M J Nunes A N Carvalho C S á-Lemos M Cola ço I Cervenka V Ciraci S G Santos M M Ribeiro M Castanheira P R Jannig M J Gama M Castro-Caldas C M P Rodrigues E Rodrigues J L Ruas Source Type: research
Sustained PGC-1 α2 or PGC-1α3 expression induces astrocyte dysfunction and degeneration
Eur J Cell Biol. 2023 Nov 19;103(1):151377. doi: 10.1016/j.ejcb.2023.151377. Online ahead of print.ABSTRACTPeroxisome proliferator-activated receptor-gamma coactivator-1α (PGC-1α) transcriptional coactivators are key regulators of energy metabolism-related genes and are expressed in energy-demanding tissues. There are several PGC-1α variants with different biological functions in different tissues. The brain is one of the tissues where the role of PGC-1α isoforms remains less explored. Here, we used a toxin-based mouse model of Parkinson's disease (PD) and observed that the expression levels of variants PGC-1α2 and PG...
Source: European Journal of Cell Biology - November 25, 2023 Category: Cytology Authors: M J Nunes A N Carvalho C S á-Lemos M Cola ço I Cervenka V Ciraci S G Santos M M Ribeiro M Castanheira P R Jannig M J Gama M Castro-Caldas C M P Rodrigues E Rodrigues J L Ruas Source Type: research
Rel governs loser elimination during stem cell competition in the Drosophila testis
Eur J Cell Biol. 2023 Nov 18;103(1):151375. doi: 10.1016/j.ejcb.2023.151375. Online ahead of print.ABSTRACTIn the Drosophila testis, a group of stromal cells termed hub provides multiple niche signals for the surrounding germline and somatic stem cells. Stem cells of both populations compete for physical retention in the niche, and clones unable to transduce any one niche signal are rapidly eliminated from the stem cell pool by differentiation. We have mapped the transcriptomes of isolated somatic cyst stem cells and differentiated cyst cells, and found that the stem cells but not their differentiated progeny exhibit the s...
Source: European Journal of Cell Biology - November 23, 2023 Category: Cytology Authors: Silvana Hof-Michel Ljubinka Cigoja Sabina Huhn Christian B ökel Source Type: research
Rel governs loser elimination during stem cell competition in the Drosophila testis
Eur J Cell Biol. 2023 Nov 18;103(1):151375. doi: 10.1016/j.ejcb.2023.151375. Online ahead of print.ABSTRACTIn the Drosophila testis, a group of stromal cells termed hub provides multiple niche signals for the surrounding germline and somatic stem cells. Stem cells of both populations compete for physical retention in the niche, and clones unable to transduce any one niche signal are rapidly eliminated from the stem cell pool by differentiation. We have mapped the transcriptomes of isolated somatic cyst stem cells and differentiated cyst cells, and found that the stem cells but not their differentiated progeny exhibit the s...
Source: European Journal of Cell Biology - November 23, 2023 Category: Cytology Authors: Silvana Hof-Michel Ljubinka Cigoja Sabina Huhn Christian B ökel Source Type: research
Rel governs loser elimination during stem cell competition in the Drosophila testis
Eur J Cell Biol. 2023 Nov 18;103(1):151375. doi: 10.1016/j.ejcb.2023.151375. Online ahead of print.ABSTRACTIn the Drosophila testis, a group of stromal cells termed hub provides multiple niche signals for the surrounding germline and somatic stem cells. Stem cells of both populations compete for physical retention in the niche, and clones unable to transduce any one niche signal are rapidly eliminated from the stem cell pool by differentiation. We have mapped the transcriptomes of isolated somatic cyst stem cells and differentiated cyst cells, and found that the stem cells but not their differentiated progeny exhibit the s...
Source: European Journal of Cell Biology - November 23, 2023 Category: Cytology Authors: Silvana Hof-Michel Ljubinka Cigoja Sabina Huhn Christian B ökel Source Type: research
Extracellular vesicles on the move: Traversing the complex matrix of tissues
Eur J Cell Biol. 2023 Dec;102(4):151372. doi: 10.1016/j.ejcb.2023.151372. Epub 2023 Nov 11.ABSTRACTExtracellular vesicles are small particles involved in intercellular signaling. They are produced by virtually all cell types, transport biological molecules, and are released into the extracellular space. Studies on extracellular vesicles have become more numerous in recent years, leading to promising research on their potential impact on health and disease. Despite significant progress in understanding the bioactivity of extracellular vesicles, most in vitro and in vivo studies overlook their transport through the extracell...
Source: European Journal of Cell Biology - November 16, 2023 Category: Cytology Authors: Syrine Arif V éronique J Moulin Source Type: research
Extracellular vesicles on the move: Traversing the complex matrix of tissues
Eur J Cell Biol. 2023 Dec;102(4):151372. doi: 10.1016/j.ejcb.2023.151372. Epub 2023 Nov 11.ABSTRACTExtracellular vesicles are small particles involved in intercellular signaling. They are produced by virtually all cell types, transport biological molecules, and are released into the extracellular space. Studies on extracellular vesicles have become more numerous in recent years, leading to promising research on their potential impact on health and disease. Despite significant progress in understanding the bioactivity of extracellular vesicles, most in vitro and in vivo studies overlook their transport through the extracell...
Source: European Journal of Cell Biology - November 16, 2023 Category: Cytology Authors: Syrine Arif V éronique J Moulin Source Type: research
Extracellular vesicles on the move: Traversing the complex matrix of tissues
Eur J Cell Biol. 2023 Nov 11;102(4):151372. doi: 10.1016/j.ejcb.2023.151372. Online ahead of print.ABSTRACTExtracellular vesicles are small particles involved in intercellular signaling. They are produced by virtually all cell types, transport biological molecules, and are released into the extracellular space. Studies on extracellular vesicles have become more numerous in recent years, leading to promising research on their potential impact on health and disease. Despite significant progress in understanding the bioactivity of extracellular vesicles, most in vitro and in vivo studies overlook their transport through the e...
Source: European Journal of Cell Biology - November 16, 2023 Category: Cytology Authors: Syrine Arif V éronique J Moulin Source Type: research
Extracellular vesicles on the move: Traversing the complex matrix of tissues
Eur J Cell Biol. 2023 Nov 11;102(4):151372. doi: 10.1016/j.ejcb.2023.151372. Online ahead of print.ABSTRACTExtracellular vesicles are small particles involved in intercellular signaling. They are produced by virtually all cell types, transport biological molecules, and are released into the extracellular space. Studies on extracellular vesicles have become more numerous in recent years, leading to promising research on their potential impact on health and disease. Despite significant progress in understanding the bioactivity of extracellular vesicles, most in vitro and in vivo studies overlook their transport through the e...
Source: European Journal of Cell Biology - November 16, 2023 Category: Cytology Authors: Syrine Arif V éronique J Moulin Source Type: research
