Leptin deficiency impairs adipogenesis and browning response in mouse mesenchymal progenitors
Eur J Cell Biol. 2023 Jul 13;102(3):151342. doi: 10.1016/j.ejcb.2023.151342. Online ahead of print.ABSTRACTAlthough phenotypically different, brown adipose tissue (BAT) and inguinal white adipose tissue (iWAT) are able to produce heat through non-shivering thermogenesis due to the presence of mitochondrial uncoupling protein 1 (UCP1). The appearance of thermogenically active beige adipocytes in iWAT is known as browning. Both brown and beige cells originate from mesenchymal stem cells (MSCs), and in culture conditions a browning response can be induced with hypothermia (i.e. 32 °C) during which nuclear leptin immunodetect...
Source: European Journal of Cell Biology - July 19, 2023 Category: Cytology Authors: Ksenija Velickovic Hilda Anaid Lugo Leija Bojana Kosic Harold Sacks Michael E Symonds Virginie Sottile Source Type: research
Leptin deficiency impairs adipogenesis and browning response in mouse mesenchymal progenitors
Eur J Cell Biol. 2023 Jul 13;102(3):151342. doi: 10.1016/j.ejcb.2023.151342. Online ahead of print.ABSTRACTAlthough phenotypically different, brown adipose tissue (BAT) and inguinal white adipose tissue (iWAT) are able to produce heat through non-shivering thermogenesis due to the presence of mitochondrial uncoupling protein 1 (UCP1). The appearance of thermogenically active beige adipocytes in iWAT is known as browning. Both brown and beige cells originate from mesenchymal stem cells (MSCs), and in culture conditions a browning response can be induced with hypothermia (i.e. 32 °C) during which nuclear leptin immunodetect...
Source: European Journal of Cell Biology - July 19, 2023 Category: Cytology Authors: Ksenija Velickovic Hilda Anaid Lugo Leija Bojana Kosic Harold Sacks Michael E Symonds Virginie Sottile Source Type: research
ING1 inhibits Twist1 expression to block EMT and is antagonized by the HDAC inhibitor vorinostat
Eur J Cell Biol. 2023 Jul 13;102(3):151341. doi: 10.1016/j.ejcb.2023.151341. Online ahead of print.ABSTRACTING1 is a chromatin targeting subunit of the Sin3a histone deacetylase (HDAC) complex that alters chromatin structure to subsequently regulate gene expression. We find that ING1 knockdown increases expression of Twist1, Zeb 1&2, Snai1, Bmi1 and TSHZ1 drivers of EMT, promoting EMT and cell motility. ING1 expression had the opposite effect, promoting epithelial cell morphology and inhibiting basal and TGF-β-induced motility in 3D organoid cultures. ING1 binds the Twist1 promoter and Twist1 was largely responsible f...
Source: European Journal of Cell Biology - July 17, 2023 Category: Cytology Authors: Yang Yang Biao Ma Mahbod Djamshidi Qingrun Zhang Anusi Sarkar Ayan Chanda Uyen Tran Jung Soh Christina Sandall Huey-Miin Chen Justin A MacDonald Shirin Bonni Christoph W Sensen Jianhua Zheng Karl Riabowol Source Type: research
ING1 inhibits Twist1 expression to block EMT and is antagonized by the HDAC inhibitor vorinostat
Eur J Cell Biol. 2023 Jul 13;102(3):151341. doi: 10.1016/j.ejcb.2023.151341. Online ahead of print.ABSTRACTING1 is a chromatin targeting subunit of the Sin3a histone deacetylase (HDAC) complex that alters chromatin structure to subsequently regulate gene expression. We find that ING1 knockdown increases expression of Twist1, Zeb 1&2, Snai1, Bmi1 and TSHZ1 drivers of EMT, promoting EMT and cell motility. ING1 expression had the opposite effect, promoting epithelial cell morphology and inhibiting basal and TGF-β-induced motility in 3D organoid cultures. ING1 binds the Twist1 promoter and Twist1 was largely responsible f...
Source: European Journal of Cell Biology - July 17, 2023 Category: Cytology Authors: Yang Yang Biao Ma Mahbod Djamshidi Qingrun Zhang Anusi Sarkar Ayan Chanda Uyen Tran Jung Soh Christina Sandall Huey-Miin Chen Justin A MacDonald Shirin Bonni Christoph W Sensen Jianhua Zheng Karl Riabowol Source Type: research
Receptor-mediated internalization promotes increased endosome size and number in a RAB4- and RAB5-dependent manner
Eur J Cell Biol. 2023 Jul 6;102(3):151339. doi: 10.1016/j.ejcb.2023.151339. Online ahead of print.ABSTRACTDespite their significance in receptor-mediated internalization and continued signal transduction in cells, early/sorting endosomes (EE/SE) remain incompletely characterized, with many outstanding questions that surround the dynamics of their size and number. While several studies have reported increases in EE/SE size and number resulting from endocytic events, few studies have addressed such dynamics in a methodological and quantitative manner. Herein we apply quantitative fluorescence microscopy to measure the size a...
Source: European Journal of Cell Biology - July 9, 2023 Category: Cytology Authors: Naava Naslavsky Steve Caplan Source Type: research
Cancer cell-specific cGAS/STING Signaling pathway in the era of advancing cancer cell biology
This article further discusses different approaches to specifically target cGAS/STING signaling in cancer cells to inhibit tumor growth and metastasis in conjunction with existing anticancer therapies.PMID:37423035 | DOI:10.1016/j.ejcb.2023.151338 (Source: European Journal of Cell Biology)
Source: European Journal of Cell Biology - July 9, 2023 Category: Cytology Authors: Vijay Kumar Caitlin Bauer John H Stewart Source Type: research
Clones of aging: When better fitness can be dangerous
Eur J Cell Biol. 2023 Jul 6;102(3):151340. doi: 10.1016/j.ejcb.2023.151340. Online ahead of print.ABSTRACTThe biological and clinical significance of aberrant clonal expansions in aged tissues is being intensely discussed. Evidence is accruing that these clones often result from the normal dynamics of cell turnover in our tissues. The aged tissue microenvironment is prone to favour the emergence of specific clones with higher fitness partly because of an overall decline in cell intrinsic regenerative potential of surrounding counterparts. Thus, expanding clones in aged tissues need not to be mechanistically associated with...
Source: European Journal of Cell Biology - July 9, 2023 Category: Cytology Authors: Fabio Marongiu Samuele Cheri Ezio Laconi Source Type: research
Receptor-mediated internalization promotes increased endosome size and number in a RAB4- and RAB5-dependent manner
Eur J Cell Biol. 2023 Jul 6;102(3):151339. doi: 10.1016/j.ejcb.2023.151339. Online ahead of print.ABSTRACTDespite their significance in receptor-mediated internalization and continued signal transduction in cells, early/sorting endosomes (EE/SE) remain incompletely characterized, with many outstanding questions that surround the dynamics of their size and number. While several studies have reported increases in EE/SE size and number resulting from endocytic events, few studies have addressed such dynamics in a methodological and quantitative manner. Herein we apply quantitative fluorescence microscopy to measure the size a...
Source: European Journal of Cell Biology - July 9, 2023 Category: Cytology Authors: Naava Naslavsky Steve Caplan Source Type: research
Cancer cell-specific cGAS/STING Signaling pathway in the era of advancing cancer cell biology
This article further discusses different approaches to specifically target cGAS/STING signaling in cancer cells to inhibit tumor growth and metastasis in conjunction with existing anticancer therapies.PMID:37423035 | DOI:10.1016/j.ejcb.2023.151338 (Source: European Journal of Cell Biology)
Source: European Journal of Cell Biology - July 9, 2023 Category: Cytology Authors: Vijay Kumar Caitlin Bauer John H Stewart Source Type: research
Clones of aging: When better fitness can be dangerous
Eur J Cell Biol. 2023 Jul 6;102(3):151340. doi: 10.1016/j.ejcb.2023.151340. Online ahead of print.ABSTRACTThe biological and clinical significance of aberrant clonal expansions in aged tissues is being intensely discussed. Evidence is accruing that these clones often result from the normal dynamics of cell turnover in our tissues. The aged tissue microenvironment is prone to favour the emergence of specific clones with higher fitness partly because of an overall decline in cell intrinsic regenerative potential of surrounding counterparts. Thus, expanding clones in aged tissues need not to be mechanistically associated with...
Source: European Journal of Cell Biology - July 9, 2023 Category: Cytology Authors: Fabio Marongiu Samuele Cheri Ezio Laconi Source Type: research
Receptor-mediated internalization promotes increased endosome size and number in a RAB4- and RAB5-dependent manner
Eur J Cell Biol. 2023 Jul 6;102(3):151339. doi: 10.1016/j.ejcb.2023.151339. Online ahead of print.ABSTRACTDespite their significance in receptor-mediated internalization and continued signal transduction in cells, early/sorting endosomes (EE/SE) remain incompletely characterized, with many outstanding questions that surround the dynamics of their size and number. While several studies have reported increases in EE/SE size and number resulting from endocytic events, few studies have addressed such dynamics in a methodological and quantitative manner. Herein we apply quantitative fluorescence microscopy to measure the size a...
Source: European Journal of Cell Biology - July 9, 2023 Category: Cytology Authors: Naava Naslavsky Steve Caplan Source Type: research
Cancer cell-specific cGAS/STING Signaling pathway in the era of advancing cancer cell biology
This article further discusses different approaches to specifically target cGAS/STING signaling in cancer cells to inhibit tumor growth and metastasis in conjunction with existing anticancer therapies.PMID:37423035 | DOI:10.1016/j.ejcb.2023.151338 (Source: European Journal of Cell Biology)
Source: European Journal of Cell Biology - July 9, 2023 Category: Cytology Authors: Vijay Kumar Caitlin Bauer John H Stewart Source Type: research
Clones of aging: When better fitness can be dangerous
Eur J Cell Biol. 2023 Jul 6;102(3):151340. doi: 10.1016/j.ejcb.2023.151340. Online ahead of print.ABSTRACTThe biological and clinical significance of aberrant clonal expansions in aged tissues is being intensely discussed. Evidence is accruing that these clones often result from the normal dynamics of cell turnover in our tissues. The aged tissue microenvironment is prone to favour the emergence of specific clones with higher fitness partly because of an overall decline in cell intrinsic regenerative potential of surrounding counterparts. Thus, expanding clones in aged tissues need not to be mechanistically associated with...
Source: European Journal of Cell Biology - July 9, 2023 Category: Cytology Authors: Fabio Marongiu Samuele Cheri Ezio Laconi Source Type: research
Receptor-mediated internalization promotes increased endosome size and number in a RAB4- and RAB5-dependent manner
Eur J Cell Biol. 2023 Jul 6;102(3):151339. doi: 10.1016/j.ejcb.2023.151339. Online ahead of print.ABSTRACTDespite their significance in receptor-mediated internalization and continued signal transduction in cells, early/sorting endosomes (EE/SE) remain incompletely characterized, with many outstanding questions that surround the dynamics of their size and number. While several studies have reported increases in EE/SE size and number resulting from endocytic events, few studies have addressed such dynamics in a methodological and quantitative manner. Herein we apply quantitative fluorescence microscopy to measure the size a...
Source: European Journal of Cell Biology - July 9, 2023 Category: Cytology Authors: Naava Naslavsky Steve Caplan Source Type: research
Cancer cell-specific cGAS/STING Signaling pathway in the era of advancing cancer cell biology
This article further discusses different approaches to specifically target cGAS/STING signaling in cancer cells to inhibit tumor growth and metastasis in conjunction with existing anticancer therapies.PMID:37423035 | DOI:10.1016/j.ejcb.2023.151338 (Source: European Journal of Cell Biology)
Source: European Journal of Cell Biology - July 9, 2023 Category: Cytology Authors: Vijay Kumar Caitlin Bauer John H Stewart Source Type: research
