Regulation of integrin α5β1-mediated Staphylococcus aureus cellular invasion by the septin cytoskeleton
Eur J Cell Biol. 2023 Sep 3;102(4):151359. doi: 10.1016/j.ejcb.2023.151359. Online ahead of print.ABSTRACTStaphylococcus aureus, a Gram-positive bacterial pathogen, is an urgent health threat causing a wide range of clinical infections. Originally viewed as a strict extracellular pathogen, accumulating evidence has revealed S. aureus to be a facultative intracellular pathogen subverting host cell signalling to support invasion. The majority of clinical isolates produce fibronectin-binding proteins A and B (FnBPA and FnBPB) to interact with host integrin α5β1, a key component of focal adhesions. S. aureus binding of integ...
Source: European Journal of Cell Biology - September 8, 2023 Category: Cytology Authors: Stevens Robertin Dominik Brokatzky Dami án Lobato-Márquez Serge Mostowy Source Type: research
Regulation of integrin α5β1-mediated Staphylococcus aureus cellular invasion by the septin cytoskeleton
Eur J Cell Biol. 2023 Sep 3;102(4):151359. doi: 10.1016/j.ejcb.2023.151359. Online ahead of print.ABSTRACTStaphylococcus aureus, a Gram-positive bacterial pathogen, is an urgent health threat causing a wide range of clinical infections. Originally viewed as a strict extracellular pathogen, accumulating evidence has revealed S. aureus to be a facultative intracellular pathogen subverting host cell signalling to support invasion. The majority of clinical isolates produce fibronectin-binding proteins A and B (FnBPA and FnBPB) to interact with host integrin α5β1, a key component of focal adhesions. S. aureus binding of integ...
Source: European Journal of Cell Biology - September 8, 2023 Category: Cytology Authors: Stevens Robertin Dominik Brokatzky Dami án Lobato-Márquez Serge Mostowy Source Type: research
Visualizing the subcellular localization of RHOB-GTP and GTPase-Effector complexes using a split-GFP/nanobody labelling assay
Eur J Cell Biol. 2023 Aug 21;102(4):151355. doi: 10.1016/j.ejcb.2023.151355. Online ahead of print.ABSTRACTSmall GTPases are highly regulated proteins that control essential signaling pathways through the activity of their effector proteins. Among the RHOA subfamily, RHOB regulates peculiar functions that could be associated with the control of the endocytic trafficking of signaling proteins. Here, we used an optimized assay based on tripartite split-GFP complementation to localize GTPase-effector complexes with high-resolution. The detection of RHOB interaction with the Rhotekin Rho binding domain (RBD) that specifically ...
Source: European Journal of Cell Biology - August 28, 2023 Category: Cytology Authors: Sebastian Castillo R émi Gence Delphine Pagan Faten Kora ïchi Catherine Bouchenot Benoit J Pons Betty Bo ëlle Aur élien Olichon Isabelle Lajoie-Mazenc Gilles Favre Jean-Denis P édelacq St éphanie Cabantous Source Type: research
Visualizing the subcellular localization of RHOB-GTP and GTPase-Effector complexes using a split-GFP/nanobody labelling assay
Eur J Cell Biol. 2023 Aug 21;102(4):151355. doi: 10.1016/j.ejcb.2023.151355. Online ahead of print.ABSTRACTSmall GTPases are highly regulated proteins that control essential signaling pathways through the activity of their effector proteins. Among the RHOA subfamily, RHOB regulates peculiar functions that could be associated with the control of the endocytic trafficking of signaling proteins. Here, we used an optimized assay based on tripartite split-GFP complementation to localize GTPase-effector complexes with high-resolution. The detection of RHOB interaction with the Rhotekin Rho binding domain (RBD) that specifically ...
Source: European Journal of Cell Biology - August 28, 2023 Category: Cytology Authors: Sebastian Castillo R émi Gence Delphine Pagan Faten Kora ïchi Catherine Bouchenot Benoit J Pons Betty Bo ëlle Aur élien Olichon Isabelle Lajoie-Mazenc Gilles Favre Jean-Denis P édelacq St éphanie Cabantous Source Type: research
Visualizing the subcellular localization of RHOB-GTP and GTPase-Effector complexes using a split-GFP/nanobody labelling assay
Eur J Cell Biol. 2023 Aug 21;102(4):151355. doi: 10.1016/j.ejcb.2023.151355. Online ahead of print.ABSTRACTSmall GTPases are highly regulated proteins that control essential signaling pathways through the activity of their effector proteins. Among the RHOA subfamily, RHOB regulates peculiar functions that could be associated with the control of the endocytic trafficking of signaling proteins. Here, we used an optimized assay based on tripartite split-GFP complementation to localize GTPase-effector complexes with high-resolution. The detection of RHOB interaction with the Rhotekin Rho binding domain (RBD) that specifically ...
Source: European Journal of Cell Biology - August 28, 2023 Category: Cytology Authors: Sebastian Castillo R émi Gence Delphine Pagan Faten Kora ïchi Catherine Bouchenot Benoit J Pons Betty Bo ëlle Aur élien Olichon Isabelle Lajoie-Mazenc Gilles Favre Jean-Denis P édelacq St éphanie Cabantous Source Type: research
Visualizing the subcellular localization of RHOB-GTP and GTPase-Effector complexes using a split-GFP/nanobody labelling assay
Eur J Cell Biol. 2023 Aug 21;102(4):151355. doi: 10.1016/j.ejcb.2023.151355. Online ahead of print.ABSTRACTSmall GTPases are highly regulated proteins that control essential signaling pathways through the activity of their effector proteins. Among the RHOA subfamily, RHOB regulates peculiar functions that could be associated with the control of the endocytic trafficking of signaling proteins. Here, we used an optimized assay based on tripartite split-GFP complementation to localize GTPase-effector complexes with high-resolution. The detection of RHOB interaction with the Rhotekin Rho binding domain (RBD) that specifically ...
Source: European Journal of Cell Biology - August 28, 2023 Category: Cytology Authors: Sebastian Castillo R émi Gence Delphine Pagan Faten Kora ïchi Catherine Bouchenot Benoit J Pons Betty Bo ëlle Aur élien Olichon Isabelle Lajoie-Mazenc Gilles Favre Jean-Denis P édelacq St éphanie Cabantous Source Type: research
Visualizing the subcellular localization of RHOB-GTP and GTPase-Effector complexes using a split-GFP/nanobody labelling assay
Eur J Cell Biol. 2023 Aug 21;102(4):151355. doi: 10.1016/j.ejcb.2023.151355. Online ahead of print.ABSTRACTSmall GTPases are highly regulated proteins that control essential signaling pathways through the activity of their effector proteins. Among the RHOA subfamily, RHOB regulates peculiar functions that could be associated with the control of the endocytic trafficking of signaling proteins. Here, we used an optimized assay based on tripartite split-GFP complementation to localize GTPase-effector complexes with high-resolution. The detection of RHOB interaction with the Rhotekin Rho binding domain (RBD) that specifically ...
Source: European Journal of Cell Biology - August 28, 2023 Category: Cytology Authors: Sebastian Castillo R émi Gence Delphine Pagan Faten Kora ïchi Catherine Bouchenot Benoit J Pons Betty Bo ëlle Aur élien Olichon Isabelle Lajoie-Mazenc Gilles Favre Jean-Denis P édelacq St éphanie Cabantous Source Type: research
Visualizing the subcellular localization of RHOB-GTP and GTPase-Effector complexes using a split-GFP/nanobody labelling assay
Eur J Cell Biol. 2023 Aug 21;102(4):151355. doi: 10.1016/j.ejcb.2023.151355. Online ahead of print.ABSTRACTSmall GTPases are highly regulated proteins that control essential signaling pathways through the activity of their effector proteins. Among the RHOA subfamily, RHOB regulates peculiar functions that could be associated with the control of the endocytic trafficking of signaling proteins. Here, we used an optimized assay based on tripartite split-GFP complementation to localize GTPase-effector complexes with high-resolution. The detection of RHOB interaction with the Rhotekin Rho binding domain (RBD) that specifically ...
Source: European Journal of Cell Biology - August 28, 2023 Category: Cytology Authors: Sebastian Castillo R émi Gence Delphine Pagan Faten Kora ïchi Catherine Bouchenot Benoit J Pons Betty Bo ëlle Aur élien Olichon Isabelle Lajoie-Mazenc Gilles Favre Jean-Denis P édelacq St éphanie Cabantous Source Type: research
Visualizing the subcellular localization of RHOB-GTP and GTPase-Effector complexes using a split-GFP/nanobody labelling assay
Eur J Cell Biol. 2023 Aug 21;102(4):151355. doi: 10.1016/j.ejcb.2023.151355. Online ahead of print.ABSTRACTSmall GTPases are highly regulated proteins that control essential signaling pathways through the activity of their effector proteins. Among the RHOA subfamily, RHOB regulates peculiar functions that could be associated with the control of the endocytic trafficking of signaling proteins. Here, we used an optimized assay based on tripartite split-GFP complementation to localize GTPase-effector complexes with high-resolution. The detection of RHOB interaction with the Rhotekin Rho binding domain (RBD) that specifically ...
Source: European Journal of Cell Biology - August 28, 2023 Category: Cytology Authors: Sebastian Castillo R émi Gence Delphine Pagan Faten Kora ïchi Catherine Bouchenot Benoit J Pons Betty Bo ëlle Aur élien Olichon Isabelle Lajoie-Mazenc Gilles Favre Jean-Denis P édelacq St éphanie Cabantous Source Type: research
Visualizing the subcellular localization of RHOB-GTP and GTPase-Effector complexes using a split-GFP/nanobody labelling assay
Eur J Cell Biol. 2023 Aug 21;102(4):151355. doi: 10.1016/j.ejcb.2023.151355. Online ahead of print.ABSTRACTSmall GTPases are highly regulated proteins that control essential signaling pathways through the activity of their effector proteins. Among the RHOA subfamily, RHOB regulates peculiar functions that could be associated with the control of the endocytic trafficking of signaling proteins. Here, we used an optimized assay based on tripartite split-GFP complementation to localize GTPase-effector complexes with high-resolution. The detection of RHOB interaction with the Rhotekin Rho binding domain (RBD) that specifically ...
Source: European Journal of Cell Biology - August 28, 2023 Category: Cytology Authors: Sebastian Castillo R émi Gence Delphine Pagan Faten Kora ïchi Catherine Bouchenot Benoit J Pons Betty Bo ëlle Aur élien Olichon Isabelle Lajoie-Mazenc Gilles Favre Jean-Denis P édelacq St éphanie Cabantous Source Type: research
Visualizing the subcellular localization of RHOB-GTP and GTPase-Effector complexes using a split-GFP/nanobody labelling assay
Eur J Cell Biol. 2023 Aug 21;102(4):151355. doi: 10.1016/j.ejcb.2023.151355. Online ahead of print.ABSTRACTSmall GTPases are highly regulated proteins that control essential signaling pathways through the activity of their effector proteins. Among the RHOA subfamily, RHOB regulates peculiar functions that could be associated with the control of the endocytic trafficking of signaling proteins. Here, we used an optimized assay based on tripartite split-GFP complementation to localize GTPase-effector complexes with high-resolution. The detection of RHOB interaction with the Rhotekin Rho binding domain (RBD) that specifically ...
Source: European Journal of Cell Biology - August 28, 2023 Category: Cytology Authors: Sebastian Castillo R émi Gence Delphine Pagan Faten Kora ïchi Catherine Bouchenot Benoit J Pons Betty Bo ëlle Aur élien Olichon Isabelle Lajoie-Mazenc Gilles Favre Jean-Denis P édelacq St éphanie Cabantous Source Type: research
Loss of the actin regulator cyclase-associated protein 1 (CAP1) modestly affects dendritic spine remodeling during synaptic plasticity
Eur J Cell Biol. 2023 Aug 24;102(4):151357. doi: 10.1016/j.ejcb.2023.151357. Online ahead of print.ABSTRACTDendritic spines form the postsynaptic compartment of most excitatory synapses in the vertebrate brain. Morphological changes of dendritic spines contribute to major forms of synaptic plasticity such as long-term potentiation (LTP) or depression (LTD). Synaptic plasticity underlies learning and memory, and defects in synaptic plasticity contribute to the pathogeneses of human brain disorders. Hence, deciphering the molecules that drive spine remodeling during synaptic plasticity is critical for understanding the neuro...
Source: European Journal of Cell Biology - August 27, 2023 Category: Cytology Authors: Anika Heinze Marco B Rust Source Type: research
Loss of the actin regulator cyclase-associated protein 1 (CAP1) modestly affects dendritic spine remodeling during synaptic plasticity
Eur J Cell Biol. 2023 Aug 24;102(4):151357. doi: 10.1016/j.ejcb.2023.151357. Online ahead of print.ABSTRACTDendritic spines form the postsynaptic compartment of most excitatory synapses in the vertebrate brain. Morphological changes of dendritic spines contribute to major forms of synaptic plasticity such as long-term potentiation (LTP) or depression (LTD). Synaptic plasticity underlies learning and memory, and defects in synaptic plasticity contribute to the pathogeneses of human brain disorders. Hence, deciphering the molecules that drive spine remodeling during synaptic plasticity is critical for understanding the neuro...
Source: European Journal of Cell Biology - August 27, 2023 Category: Cytology Authors: Anika Heinze Marco B Rust Source Type: research
Get a grip: Podosomes as potential players in phagocytosis
Eur J Cell Biol. 2023 Aug 22;102(4):151356. doi: 10.1016/j.ejcb.2023.151356. Online ahead of print.ABSTRACTPodosomes have been known for several decades as micron-sized, F-actin-rich structures that play a pivotal role in cell migration and invasion, as they are able to mediate both cell-matrix attachment as well as extracellular matrix degradation. Particularly in monocytic cells, podosomes have been shown to fulfill a variety of additional functions such as sensing of substrate rigidity and topography, or cell-cell fusion. Increasing evidence now points to the involvement of podosome-like structures also during phagocyto...
Source: European Journal of Cell Biology - August 25, 2023 Category: Cytology Authors: Stefan Linder Bryan Barcelona Source Type: research
Get a grip: Podosomes as potential players in phagocytosis
Eur J Cell Biol. 2023 Aug 22;102(4):151356. doi: 10.1016/j.ejcb.2023.151356. Online ahead of print.ABSTRACTPodosomes have been known for several decades as micron-sized, F-actin-rich structures that play a pivotal role in cell migration and invasion, as they are able to mediate both cell-matrix attachment as well as extracellular matrix degradation. Particularly in monocytic cells, podosomes have been shown to fulfill a variety of additional functions such as sensing of substrate rigidity and topography, or cell-cell fusion. Increasing evidence now points to the involvement of podosome-like structures also during phagocyto...
Source: European Journal of Cell Biology - August 25, 2023 Category: Cytology Authors: Stefan Linder Bryan Barcelona Source Type: research
