Sucrose phosphorylase from Alteromonas mediterranea: Structural insight into the regioselective α-glucosylation of (+)-catechin
Biochimie. 2024 Jan 9;221:13-19. doi: 10.1016/j.biochi.2024.01.004. Online ahead of print.ABSTRACTSucrose phosphorylases, through transglycosylation reactions, are interesting enzymes that can transfer regioselectively glucose from sucrose, the donor substrate, onto acceptors like flavonoids to form glycoconjugates and hence modulate their solubility and bioactivity. Here, we report for the first time the structure of sucrose phosphorylase from the marine bacteria Alteromonas mediterranea (AmSP) and its enzymatic properties. Kinetics of sucrose hydrolysis and transglucosylation capacities on (+)-catechin were investigated....
Source: Biochimie - January 10, 2024 Category: Biochemistry Authors: Marine Goux Marie Demonceaux Johann Hendrickx Claude Solleux Emilie Lormeau Folmer Fredslund David Tez é Bernard Offmann Corinne Andr é-Miral Source Type: research

The determinants of metabolic discrepancies in aerobic glycolysis: Providing potential targets for breast cancer treatment
Biochimie. 2024 Jan 4;220:107-121. doi: 10.1016/j.biochi.2024.01.003. Online ahead of print.ABSTRACTAltered aerobic glycolysis is the robust mechanism to support cancer cell survival and proliferation beyond the maintenance of cellular energy metabolism. Several investigators portrayed the important role of deregulated glycolysis in different cancers, including breast cancer. Breast cancer is the most ubiquitous form of cancer and the primary cause of cancer death in women worldwide. Breast cancer with increased glycolytic flux is hampered to eradicate with current therapies and can result in tumor recurrence. In spite of ...
Source: Biochimie - January 6, 2024 Category: Biochemistry Authors: Ajeesh Babu Littleflower Sulfath Thottungal Parambil Gisha Rose Antony Lakshmi Subhadradevi Source Type: research

The determinants of metabolic discrepancies in aerobic glycolysis: Providing potential targets for breast cancer treatment
Biochimie. 2024 Jan 4:S0300-9084(24)00003-8. doi: 10.1016/j.biochi.2024.01.003. Online ahead of print.ABSTRACTAltered aerobic glycolysis is the robust mechanism to support cancer cell survival and proliferation beyond the maintenance of cellular energy metabolism. Several investigators portrayed the important role of deregulated glycolysis in different cancers, including breast cancer. Breast cancer is the most ubiquitous form of cancer and the primary cause of cancer death in women worldwide. Breast cancer with increased glycolytic flux is hampered to eradicate with current therapies and can result in tumor recurrence. In...
Source: Biochimie - January 6, 2024 Category: Biochemistry Authors: Ajeesh Babu Littleflower Sulfath Thottungal Parambil Gisha Rose Antony Lakshmi Subhadradevi Source Type: research

The determinants of metabolic discrepancies in aerobic glycolysis: Providing potential targets for breast cancer treatment
Biochimie. 2024 Jan 4:S0300-9084(24)00003-8. doi: 10.1016/j.biochi.2024.01.003. Online ahead of print.ABSTRACTAltered aerobic glycolysis is the robust mechanism to support cancer cell survival and proliferation beyond the maintenance of cellular energy metabolism. Several investigators portrayed the important role of deregulated glycolysis in different cancers, including breast cancer. Breast cancer is the most ubiquitous form of cancer and the primary cause of cancer death in women worldwide. Breast cancer with increased glycolytic flux is hampered to eradicate with current therapies and can result in tumor recurrence. In...
Source: Biochimie - January 6, 2024 Category: Biochemistry Authors: Ajeesh Babu Littleflower Sulfath Thottungal Parambil Gisha Rose Antony Lakshmi Subhadradevi Source Type: research

The determinants of metabolic discrepancies in aerobic glycolysis: Providing potential targets for breast cancer treatment
Biochimie. 2024 Jan 4:S0300-9084(24)00003-8. doi: 10.1016/j.biochi.2024.01.003. Online ahead of print.ABSTRACTAltered aerobic glycolysis is the robust mechanism to support cancer cell survival and proliferation beyond the maintenance of cellular energy metabolism. Several investigators portrayed the important role of deregulated glycolysis in different cancers, including breast cancer. Breast cancer is the most ubiquitous form of cancer and the primary cause of cancer death in women worldwide. Breast cancer with increased glycolytic flux is hampered to eradicate with current therapies and can result in tumor recurrence. In...
Source: Biochimie - January 6, 2024 Category: Biochemistry Authors: Ajeesh Babu Littleflower Sulfath Thottungal Parambil Gisha Rose Antony Lakshmi Subhadradevi Source Type: research

Exploring NAD < sup > + < /sup > metabolism and NNAT: Insights from structure, function, and computational modeling
Biochimie. 2024 Jan 3:S0300-9084(24)00002-6. doi: 10.1016/j.biochi.2024.01.002. Online ahead of print.ABSTRACTNicotinamide Adenine Dinucleotide (NAD+), a coenzyme, is ubiquitously distributed and serves crucial functions in diverse biological processes, encompassing redox reactions, energy metabolism, and cellular signalling. This review article explores the intricate realm of NAD + metabolism, with a particular emphasis on the complex relationship between its structure, function, and the pivotal enzyme, Nicotinate Nucleotide Adenylyltransferase (NNAT), also known as nicotinate mononucleotide adenylyltransferase (NaMNAT), ...
Source: Biochimie - January 5, 2024 Category: Biochemistry Authors: Olamide Jeje Sarah Otun Chinyere Aloke Ikechukwu Achilonu Source Type: research

Recent advances in nano/microfluidics-based cell isolation techniques for cancer diagnosis and treatments
Biochimie. 2024 Jan 2:S0300-9084(24)00001-4. doi: 10.1016/j.biochi.2024.01.001. Online ahead of print.ABSTRACTMiniaturization has improved significantly in the recent decade, which has enabled the development of numerous microfluidic systems. Microfluidic technologies have shown great potential for separating desired cells from heterogeneous samples, as they offer benefits such as low sample consumption, easy operation, and high separation accuracy. Microfluidic cell separation approaches can be classified into physical (label-free) and biological (labeled) methods based on their working principles. Each method has remarka...
Source: Biochimie - January 4, 2024 Category: Biochemistry Authors: Nahid Shanehband Seyed Morteza Naghib Source Type: research

The complex repertoire of Tityus spp. venoms: Advances on their composition and pharmacological potential of their toxins
Biochimie. 2024 Jan 2:S0300-9084(23)00336-X. doi: 10.1016/j.biochi.2023.12.012. Online ahead of print.ABSTRACTAnimal venoms are a rich and complex source of components, including peptides (such as neurotoxins, anionic peptides and hypotensins), lipids, proteins (such as proteases, hyaluronidases and phospholipases) and inorganic compounds, which affect all biological systems of the envenoming victim. Their action may result in a wide range of clinical manifestations, including tachy/bradycardia, hyper/hypotension, disorders in blood coagulation, pain, edema, inflammation, fever, muscle paralysis, coma and even death. Scorp...
Source: Biochimie - January 4, 2024 Category: Biochemistry Authors: Gisele A Wiezel Isadora S Oliveira Mouzarllem B Reis Isabela G Ferreira Kalynka R Cordeiro Karla C F Bordon Eliane C Arantes Source Type: research

Recent advances in nano/microfluidics-based cell isolation techniques for cancer diagnosis and treatments
Biochimie. 2024 Jan 2:S0300-9084(24)00001-4. doi: 10.1016/j.biochi.2024.01.001. Online ahead of print.ABSTRACTMiniaturization has improved significantly in the recent decade, which has enabled the development of numerous microfluidic systems. Microfluidic technologies have shown great potential for separating desired cells from heterogeneous samples, as they offer benefits such as low sample consumption, easy operation, and high separation accuracy. Microfluidic cell separation approaches can be classified into physical (label-free) and biological (labeled) methods based on their working principles. Each method has remarka...
Source: Biochimie - January 4, 2024 Category: Biochemistry Authors: Nahid Shanehband Seyed Morteza Naghib Source Type: research

The complex repertoire of Tityus spp. venoms: Advances on their composition and pharmacological potential of their toxins
Biochimie. 2024 Jan 2:S0300-9084(23)00336-X. doi: 10.1016/j.biochi.2023.12.012. Online ahead of print.ABSTRACTAnimal venoms are a rich and complex source of components, including peptides (such as neurotoxins, anionic peptides and hypotensins), lipids, proteins (such as proteases, hyaluronidases and phospholipases) and inorganic compounds, which affect all biological systems of the envenoming victim. Their action may result in a wide range of clinical manifestations, including tachy/bradycardia, hyper/hypotension, disorders in blood coagulation, pain, edema, inflammation, fever, muscle paralysis, coma and even death. Scorp...
Source: Biochimie - January 4, 2024 Category: Biochemistry Authors: Gisele A Wiezel Isadora S Oliveira Mouzarllem B Reis Isabela G Ferreira Kalynka R Cordeiro Karla C F Bordon Eliane C Arantes Source Type: research

Unlocking the enigma of phenotypic drug tolerance: Mechanisms and emerging therapeutic strategies
Biochimie. 2023 Dec 31:S0300-9084(23)00333-4. doi: 10.1016/j.biochi.2023.12.009. Online ahead of print.ABSTRACTIn the ongoing battle against antimicrobial resistance, phenotypic drug tolerance poses a formidable challenge. This adaptive ability of microorganisms to withstand drug pressure without genetic alterations further complicating global healthcare challenges. Microbial populations employ an array of persistence mechanisms, including dormancy, biofilm formation, adaptation to intracellular environments, and the adoption of L-forms, to develop drug tolerance. Moreover, molecular mechanisms like toxin-antitoxin modules...
Source: Biochimie - January 3, 2024 Category: Biochemistry Authors: Alok K Mishra Ritesh P Thakare Bela G Santani Shivraj M Yabaji Shivendra K Dixit Kishore K Srivastava Source Type: research

Unlocking the enigma of phenotypic drug tolerance: Mechanisms and emerging therapeutic strategies
Biochimie. 2023 Dec 31:S0300-9084(23)00333-4. doi: 10.1016/j.biochi.2023.12.009. Online ahead of print.ABSTRACTIn the ongoing battle against antimicrobial resistance, phenotypic drug tolerance poses a formidable challenge. This adaptive ability of microorganisms to withstand drug pressure without genetic alterations further complicating global healthcare challenges. Microbial populations employ an array of persistence mechanisms, including dormancy, biofilm formation, adaptation to intracellular environments, and the adoption of L-forms, to develop drug tolerance. Moreover, molecular mechanisms like toxin-antitoxin modules...
Source: Biochimie - January 3, 2024 Category: Biochemistry Authors: Alok K Mishra Ritesh P Thakare Bela G Santani Shivraj M Yabaji Shivendra K Dixit Kishore K Srivastava Source Type: research

Comparison of the calcium signaling alterations in GABA-ergic medium spiny neurons produced from iPSCs of different origins
Biochimie. 2023 Dec 30:S0300-9084(23)00335-8. doi: 10.1016/j.biochi.2023.12.011. Online ahead of print.ABSTRACTDisease models based on induced pluripotent stem cells (iPSCs) are in high demand because of their physiological adequacy and well-reproducibility of the pathological phenotype. Nowadays, the most common approach to generate iPSCs is the reprogramming of somatic cells using vectors based on lentivirus or Sendai virus. We have previously shown impairments of calcium signaling including store-operated calcium entry in Huntington's disease-specific iPSCs-based GABA-ergic medium spiny neurons. However, different appro...
Source: Biochimie - January 1, 2024 Category: Biochemistry Authors: Arina A Oshkolova Dmitriy A Grekhnev Anna A Kruchinina Lilia D Belikova Egor A Volovikov Olga S Lebedeva Alexandra N Bogomazova Vladimir A Vigont Maria A Lagarkova Elena V Kaznacheyeva Source Type: research

Comparison of the calcium signaling alterations in GABA-ergic medium spiny neurons produced from iPSCs of different origins
Biochimie. 2023 Dec 30:S0300-9084(23)00335-8. doi: 10.1016/j.biochi.2023.12.011. Online ahead of print.ABSTRACTDisease models based on induced pluripotent stem cells (iPSCs) are in high demand because of their physiological adequacy and well-reproducibility of the pathological phenotype. Nowadays, the most common approach to generate iPSCs is the reprogramming of somatic cells using vectors based on lentivirus or Sendai virus. We have previously shown impairments of calcium signaling including store-operated calcium entry in Huntington's disease-specific iPSCs-based GABA-ergic medium spiny neurons. However, different appro...
Source: Biochimie - January 1, 2024 Category: Biochemistry Authors: Arina A Oshkolova Dmitriy A Grekhnev Anna A Kruchinina Lilia D Belikova Egor A Volovikov Olga S Lebedeva Alexandra N Bogomazova Vladimir A Vigont Maria A Lagarkova Elena V Kaznacheyeva Source Type: research

Potential elevation of exopeptidase activity of Glu-specific endopeptidase I/GluV8 mediated by hydrophobic P1'-position amino acid residue
Biochimie. 2023 Dec 28:S0300-9084(23)00334-6. doi: 10.1016/j.biochi.2023.12.010. Online ahead of print.ABSTRACTWe recently reported that the activities of dipeptidyl-peptidase (DPP)7 and DPP11, S46-family exopeptidases were significantly elevated by the presence of prime-side amino acid residues of substrates caused by an increase in kcat [Ohara-Nemoto Y. et al., J Biol Chem 298(3):101585. doi: 10.1016/j.jbc.2022]. In the present study, the effects of prime-side residues on Glu-specific endopeptidase I/GluV8 from Staphylococcus aureus were investigated using a two-step cleavage method with tetrapeptidyl-methycoumaryl-7-ami...
Source: Biochimie - December 30, 2023 Category: Biochemistry Authors: Takayuki K Nemoto Haruka Nishimata Kana Shirakura Yuko Ohara-Nemoto Source Type: research