Benefits of rutin on mitochondrial function and inflammation in an aluminum-induced neurotoxicity rat model: Potential interest for the prevention of neurodegeneration
In this study, we aimed to investigate the potential of rutin, a naturally occurring plant bioactive molecule, to mitigate the neurotoxic effects induced by aluminum chloride (AlCl3). Over a period of 6 weeks, rats were intraperitoneally injected with AlCl3 at a weekly dose of 60 mg/kg, while rutin treatment was administered orally via gavage at a daily dose of 30 mg/kg. AlCl3 exposure resulted in a significant increase lipid peroxidation (LPO) by 316.24%, nitrate levels by 504.14%, and tumor necrosis factor-alpha (TNF-α) levels by 93.82% in brain mitochondria. Additionally, AlCl3 exposure led to a reduction in glutathion...
Source: Biochimie - February 26, 2024 Category: Biochemistry Authors: Khadidja Kessas Wafaa Lounis Zehor Chouari Anne Vejux G érard Lizard Omar Kharoubi Source Type: research

Characterization of SLA RNA promoter from dengue virus and its interaction with the viral non-structural NS5 protein
Biochimie. 2024 Feb 24:S0300-9084(24)00043-9. doi: 10.1016/j.biochi.2024.02.005. Online ahead of print.ABSTRACTThe Dengue virus (DENV) is the most significant arthropod-borne viral pathogen in humans with 400 million infections annually. DENV comprises four distinct serotypes (DENV-1 to -4) which complicates vaccine development. Any of the four serotypes can cause clinical illness but with distinctive infection dynamics. Variations in sequences identified within the four genomes induce structural differences in crucial RNA motifs that were suggested to be correlated to the degree of pathogenicity among DENV-1 to -4. In par...
Source: Biochimie - February 26, 2024 Category: Biochemistry Authors: Karl Brillet Marta Janczuk-Richter Amanda Poon Joanne Laukart-Bradley Eric Ennifar Isabelle Lebars Source Type: research

Pyridoxal kinase gene deletion leads to impaired growth, deranged redox metabolism and cell cycle arrest in Leishmania donovani
Biochimie. 2024 Feb 23:S0300-9084(24)00047-6. doi: 10.1016/j.biochi.2024.02.009. Online ahead of print.ABSTRACTPyridoxal kinase (PdxK) is a vitamin B6 salvage pathway enzyme which produces pyridoxal phosphate. We have investigated the impact of PdxK deletion in Leishmania donovani on parasite survivability, infectivity and cellular metabolism. LdPdxK mutants were generated by gene replacement strategy. All mutants showed significant reduction in growth in comparison to wild type. For PdxK mediated biochemical perturbations, only heterozygous mutants and complementation mutants were used as the growth of null mutants were c...
Source: Biochimie - February 25, 2024 Category: Biochemistry Authors: Pradyot Kumar Roy Anindita Paul Sandra Lalchhuanawmi Neerupudi Kishore Babu Sushma Singh Source Type: research

Pyridoxal kinase gene deletion leads to impaired growth, deranged redox metabolism and cell cycle arrest in Leishmania donovani
Biochimie. 2024 Feb 23:S0300-9084(24)00047-6. doi: 10.1016/j.biochi.2024.02.009. Online ahead of print.ABSTRACTPyridoxal kinase (PdxK) is a vitamin B6 salvage pathway enzyme which produces pyridoxal phosphate. We have investigated the impact of PdxK deletion in Leishmania donovani on parasite survivability, infectivity and cellular metabolism. LdPdxK mutants were generated by gene replacement strategy. All mutants showed significant reduction in growth in comparison to wild type. For PdxK mediated biochemical perturbations, only heterozygous mutants and complementation mutants were used as the growth of null mutants were c...
Source: Biochimie - February 25, 2024 Category: Biochemistry Authors: Pradyot Kumar Roy Anindita Paul Sandra Lalchhuanawmi Neerupudi Kishore Babu Sushma Singh Source Type: research

The 'Not-So-Famous Five' in tumorigenesis: tRNAs, tRNA fragments, and tRNA epitranscriptome in concert with AARSs and AIMPs
Biochimie. 2024 Feb 22:S0300-9084(24)00042-7. doi: 10.1016/j.biochi.2024.02.004. Online ahead of print.ABSTRACTRNA profiling studies have revealed that ∼75% of the human genome is transcribed to RNA but only a meagre fraction of it is translated to proteins. Majority of transcribed RNA constitute a specialized pool of non-coding RNAs. Human genome contains approximately 506 genes encoding a set of 51 different tRNAs, constituting a unique class of non-coding RNAs that not only have essential housekeeping functions as translator molecules during protein synthesis, but have numerous uncharted regulatory functions. Intrigui...
Source: Biochimie - February 24, 2024 Category: Biochemistry Authors: Sutapa Saha Biyas Mukherjee Proma Banerjee Debadrita Das Source Type: research

Dioclea violacea lectin inhibits tumorigenesis and tumor angiogenesis in vivo
This study evaluated the cytotoxic effect of DvL on tumor and normal cells using the mitochondrial activity reduction (MTT) assay, the carcinogenic and anti-carcinogenic activity by the epithelial tumor test (ETT) in Drosophila melanogaster, and the anti-angiogenic effect by the chick embryo chorioallantoic membrane (CAM) assay. Data demonstrated that DvL promoted strong selective cytotoxicity against tumor cell lines, especially A549 and S180 cells, whereas normal cell lines were weakly affected. Furthermore, DvL did not promote carcinogenesis in D. melanogaster at any concentration tested, but modulated DXR-induced carci...
Source: Biochimie - February 23, 2024 Category: Biochemistry Authors: Abel Vieira de Melo Bisneto Amanda Silva Fernandes L ívia do Carmo Silva Luana Santos Silva Diego Pereira de Ara újo Ivan Cerqueira Dos Santos Marcella da Rocha Melo Rom ério Rodrigues Dos Santos Silva Leonardo Pereira Franchi Clever Gomes Cardoso Elis Source Type: research

Dioclea violacea lectin inhibits tumorigenesis and tumor angiogenesis in vivo
This study evaluated the cytotoxic effect of DvL on tumor and normal cells using the mitochondrial activity reduction (MTT) assay, the carcinogenic and anti-carcinogenic activity by the epithelial tumor test (ETT) in Drosophila melanogaster, and the anti-angiogenic effect by the chick embryo chorioallantoic membrane (CAM) assay. Data demonstrated that DvL promoted strong selective cytotoxicity against tumor cell lines, especially A549 and S180 cells, whereas normal cell lines were weakly affected. Furthermore, DvL did not promote carcinogenesis in D. melanogaster at any concentration tested, but modulated DXR-induced carci...
Source: Biochimie - February 23, 2024 Category: Biochemistry Authors: Abel Vieira de Melo Bisneto Amanda Silva Fernandes L ívia do Carmo Silva Luana Santos Silva Diego Pereira de Ara újo Ivan Cerqueira Dos Santos Marcella da Rocha Melo Rom ério Rodrigues Dos Santos Silva Leonardo Pereira Franchi Clever Gomes Cardoso Elis Source Type: research

The cellular SFPQ protein as a positive factor in the HIV-1 integration
Biochimie. 2024 Feb 17:S0300-9084(24)00038-5. doi: 10.1016/j.biochi.2024.02.002. Online ahead of print.ABSTRACTThe cellular SFPQ protein is involved in several stages of the HIV-1 life cycle, but the detailed mechanism of its involvement is not yet fully understood. Here, the role of SFPQ in the early stages of HIV-1 replication has been studied. It is found that changes in the intracellular level of SFPQ affect the integration of viral DNA, but not reverse transcription, and SFPQ is a positive factor of integration. A study of the SFPQ interaction with HIV-1 integrase (IN) has revealed two diRGGX1-4 motifs in the N-termin...
Source: Biochimie - February 19, 2024 Category: Biochemistry Authors: Тatiana Kikhai Yulia Agapkina Maria Silkina Tatiana Prikazchikova Marina Gottikh Source Type: research

The cellular SFPQ protein as a positive factor in the HIV-1 integration
Biochimie. 2024 Feb 17:S0300-9084(24)00038-5. doi: 10.1016/j.biochi.2024.02.002. Online ahead of print.ABSTRACTThe cellular SFPQ protein is involved in several stages of the HIV-1 life cycle, but the detailed mechanism of its involvement is not yet fully understood. Here, the role of SFPQ in the early stages of HIV-1 replication has been studied. It is found that changes in the intracellular level of SFPQ affect the integration of viral DNA, but not reverse transcription, and SFPQ is a positive factor of integration. A study of the SFPQ interaction with HIV-1 integrase (IN) has revealed two diRGGX1-4 motifs in the N-termin...
Source: Biochimie - February 19, 2024 Category: Biochemistry Authors: Тatiana Kikhai Yulia Agapkina Maria Silkina Tatiana Prikazchikova Marina Gottikh Source Type: research

The cellular SFPQ protein as a positive factor in the HIV-1 integration
Biochimie. 2024 Feb 17:S0300-9084(24)00038-5. doi: 10.1016/j.biochi.2024.02.002. Online ahead of print.ABSTRACTThe cellular SFPQ protein is involved in several stages of the HIV-1 life cycle, but the detailed mechanism of its involvement is not yet fully understood. Here, the role of SFPQ in the early stages of HIV-1 replication has been studied. It is found that changes in the intracellular level of SFPQ affect the integration of viral DNA, but not reverse transcription, and SFPQ is a positive factor of integration. A study of the SFPQ interaction with HIV-1 integrase (IN) has revealed two diRGGX1-4 motifs in the N-termin...
Source: Biochimie - February 19, 2024 Category: Biochemistry Authors: Тatiana Kikhai Yulia Agapkina Maria Silkina Tatiana Prikazchikova Marina Gottikh Source Type: research

The cellular SFPQ protein as a positive factor in the HIV-1 integration
Biochimie. 2024 Feb 17:S0300-9084(24)00038-5. doi: 10.1016/j.biochi.2024.02.002. Online ahead of print.ABSTRACTThe cellular SFPQ protein is involved in several stages of the HIV-1 life cycle, but the detailed mechanism of its involvement is not yet fully understood. Here, the role of SFPQ in the early stages of HIV-1 replication has been studied. It is found that changes in the intracellular level of SFPQ affect the integration of viral DNA, but not reverse transcription, and SFPQ is a positive factor of integration. A study of the SFPQ interaction with HIV-1 integrase (IN) has revealed two diRGGX1-4 motifs in the N-termin...
Source: Biochimie - February 19, 2024 Category: Biochemistry Authors: Тatiana Kikhai Yulia Agapkina Maria Silkina Tatiana Prikazchikova Marina Gottikh Source Type: research

Renal ischemia and reperfusion impact the purinergic signaling in a vascular bed distant from the injured site
This study aimed to investigate the impact of AKI on purinergic components in mice aorta.MAIN METHODS: The kidney ischemia was achieved by the occlusion of the left kidney pedicle for 60 min, followed by reperfusion for 8 (IR8) and 15 (IR15) days. Renal function was assessed through biochemical assays, while gene expression levels were evaluated by RT-qPCR.KEY FINDINGS: Analyses of renal parameters showed renal remodeling through mass loss in the left kidney and hypertrophy of the right kidney in the IR15 group. Furthermore, after 15 days, local inflammation was evidenced in the aorta. Moreover, the aorta purinergic compon...
Source: Biochimie - February 15, 2024 Category: Biochemistry Authors: Jeferson Stabile Raquel Silva Neres-Santos Isabela Dorta Molina Hernandes Carolina Vict ória Cruz Junho Geovane Felippe Alves Isabella Cardoso Silva Marcela Sorelli Carneiro-Ramos Cristina Ribas F ürstenau Source Type: research

Renal ischemia and reperfusion impact the purinergic signaling in a vascular bed distant from the injured site
This study aimed to investigate the impact of AKI on purinergic components in mice aorta.MAIN METHODS: The kidney ischemia was achieved by the occlusion of the left kidney pedicle for 60 min, followed by reperfusion for 8 (IR8) and 15 (IR15) days. Renal function was assessed through biochemical assays, while gene expression levels were evaluated by RT-qPCR.KEY FINDINGS: Analyses of renal parameters showed renal remodeling through mass loss in the left kidney and hypertrophy of the right kidney in the IR15 group. Furthermore, after 15 days, local inflammation was evidenced in the aorta. Moreover, the aorta purinergic compon...
Source: Biochimie - February 15, 2024 Category: Biochemistry Authors: Jeferson Stabile Raquel Silva Neres-Santos Isabela Dorta Molina Hernandes Carolina Vict ória Cruz Junho Geovane Felippe Alves Isabella Cardoso Silva Marcela Sorelli Carneiro-Ramos Cristina Ribas F ürstenau Source Type: research

Renal ischemia and reperfusion impact the purinergic signaling in a vascular bed distant from the injured site
This study aimed to investigate the impact of AKI on purinergic components in mice aorta.MAIN METHODS: The kidney ischemia was achieved by the occlusion of the left kidney pedicle for 60 min, followed by reperfusion for 8 (IR8) and 15 (IR15) days. Renal function was assessed through biochemical assays, while gene expression levels were evaluated by RT-qPCR.KEY FINDINGS: Analyses of renal parameters showed renal remodeling through mass loss in the left kidney and hypertrophy of the right kidney in the IR15 group. Furthermore, after 15 days, local inflammation was evidenced in the aorta. Moreover, the aorta purinergic compon...
Source: Biochimie - February 15, 2024 Category: Biochemistry Authors: Jeferson Stabile Raquel Silva Neres-Santos Isabela Dorta Molina Hernandes Carolina Vict ória Cruz Junho Geovane Felippe Alves Isabella Cardoso Silva Marcela Sorelli Carneiro-Ramos Cristina Ribas F ürstenau Source Type: research

Renal ischemia and reperfusion impact the purinergic signaling in a vascular bed distant from the injured site
This study aimed to investigate the impact of AKI on purinergic components in mice aorta.MAIN METHODS: The kidney ischemia was achieved by the occlusion of the left kidney pedicle for 60 min, followed by reperfusion for 8 (IR8) and 15 (IR15) days. Renal function was assessed through biochemical assays, while gene expression levels were evaluated by RT-qPCR.KEY FINDINGS: Analyses of renal parameters showed renal remodeling through mass loss in the left kidney and hypertrophy of the right kidney in the IR15 group. Furthermore, after 15 days, local inflammation was evidenced in the aorta. Moreover, the aorta purinergic compon...
Source: Biochimie - February 15, 2024 Category: Biochemistry Authors: Jeferson Stabile Raquel Silva Neres-Santos Isabela Dorta Molina Hernandes Carolina Vict ória Cruz Junho Geovane Felippe Alves Isabella Cardoso Silva Marcela Sorelli Carneiro-Ramos Cristina Ribas F ürstenau Source Type: research