The complex repertoire of Tityus spp. venoms: Advances on their composition and pharmacological potential of their toxins
Biochimie. 2024 Jan 2:S0300-9084(23)00336-X. doi: 10.1016/j.biochi.2023.12.012. Online ahead of print.ABSTRACTAnimal venoms are a rich and complex source of components, including peptides (such as neurotoxins, anionic peptides and hypotensins), lipids, proteins (such as proteases, hyaluronidases and phospholipases) and inorganic compounds, which affect all biological systems of the envenoming victim. Their action may result in a wide range of clinical manifestations, including tachy/bradycardia, hyper/hypotension, disorders in blood coagulation, pain, edema, inflammation, fever, muscle paralysis, coma and even death. Scorp...
Source: Biochimie - January 4, 2024 Category: Biochemistry Authors: Gisele A Wiezel Isadora S Oliveira Mouzarllem B Reis Isabela G Ferreira Kalynka R Cordeiro Karla C F Bordon Eliane C Arantes Source Type: research
Recent advances in nano/microfluidics-based cell isolation techniques for cancer diagnosis and treatments
Biochimie. 2024 Jan 2:S0300-9084(24)00001-4. doi: 10.1016/j.biochi.2024.01.001. Online ahead of print.ABSTRACTMiniaturization has improved significantly in the recent decade, which has enabled the development of numerous microfluidic systems. Microfluidic technologies have shown great potential for separating desired cells from heterogeneous samples, as they offer benefits such as low sample consumption, easy operation, and high separation accuracy. Microfluidic cell separation approaches can be classified into physical (label-free) and biological (labeled) methods based on their working principles. Each method has remarka...
Source: Biochimie - January 4, 2024 Category: Biochemistry Authors: Nahid Shanehband Seyed Morteza Naghib Source Type: research
The complex repertoire of Tityus spp. venoms: Advances on their composition and pharmacological potential of their toxins
Biochimie. 2024 Jan 2:S0300-9084(23)00336-X. doi: 10.1016/j.biochi.2023.12.012. Online ahead of print.ABSTRACTAnimal venoms are a rich and complex source of components, including peptides (such as neurotoxins, anionic peptides and hypotensins), lipids, proteins (such as proteases, hyaluronidases and phospholipases) and inorganic compounds, which affect all biological systems of the envenoming victim. Their action may result in a wide range of clinical manifestations, including tachy/bradycardia, hyper/hypotension, disorders in blood coagulation, pain, edema, inflammation, fever, muscle paralysis, coma and even death. Scorp...
Source: Biochimie - January 4, 2024 Category: Biochemistry Authors: Gisele A Wiezel Isadora S Oliveira Mouzarllem B Reis Isabela G Ferreira Kalynka R Cordeiro Karla C F Bordon Eliane C Arantes Source Type: research
Unlocking the enigma of phenotypic drug tolerance: Mechanisms and emerging therapeutic strategies
Biochimie. 2023 Dec 31:S0300-9084(23)00333-4. doi: 10.1016/j.biochi.2023.12.009. Online ahead of print.ABSTRACTIn the ongoing battle against antimicrobial resistance, phenotypic drug tolerance poses a formidable challenge. This adaptive ability of microorganisms to withstand drug pressure without genetic alterations further complicating global healthcare challenges. Microbial populations employ an array of persistence mechanisms, including dormancy, biofilm formation, adaptation to intracellular environments, and the adoption of L-forms, to develop drug tolerance. Moreover, molecular mechanisms like toxin-antitoxin modules...
Source: Biochimie - January 3, 2024 Category: Biochemistry Authors: Alok K Mishra Ritesh P Thakare Bela G Santani Shivraj M Yabaji Shivendra K Dixit Kishore K Srivastava Source Type: research
Unlocking the enigma of phenotypic drug tolerance: Mechanisms and emerging therapeutic strategies
Biochimie. 2023 Dec 31:S0300-9084(23)00333-4. doi: 10.1016/j.biochi.2023.12.009. Online ahead of print.ABSTRACTIn the ongoing battle against antimicrobial resistance, phenotypic drug tolerance poses a formidable challenge. This adaptive ability of microorganisms to withstand drug pressure without genetic alterations further complicating global healthcare challenges. Microbial populations employ an array of persistence mechanisms, including dormancy, biofilm formation, adaptation to intracellular environments, and the adoption of L-forms, to develop drug tolerance. Moreover, molecular mechanisms like toxin-antitoxin modules...
Source: Biochimie - January 3, 2024 Category: Biochemistry Authors: Alok K Mishra Ritesh P Thakare Bela G Santani Shivraj M Yabaji Shivendra K Dixit Kishore K Srivastava Source Type: research
Comparison of the calcium signaling alterations in GABA-ergic medium spiny neurons produced from iPSCs of different origins
Biochimie. 2023 Dec 30:S0300-9084(23)00335-8. doi: 10.1016/j.biochi.2023.12.011. Online ahead of print.ABSTRACTDisease models based on induced pluripotent stem cells (iPSCs) are in high demand because of their physiological adequacy and well-reproducibility of the pathological phenotype. Nowadays, the most common approach to generate iPSCs is the reprogramming of somatic cells using vectors based on lentivirus or Sendai virus. We have previously shown impairments of calcium signaling including store-operated calcium entry in Huntington's disease-specific iPSCs-based GABA-ergic medium spiny neurons. However, different appro...
Source: Biochimie - January 1, 2024 Category: Biochemistry Authors: Arina A Oshkolova Dmitriy A Grekhnev Anna A Kruchinina Lilia D Belikova Egor A Volovikov Olga S Lebedeva Alexandra N Bogomazova Vladimir A Vigont Maria A Lagarkova Elena V Kaznacheyeva Source Type: research
Comparison of the calcium signaling alterations in GABA-ergic medium spiny neurons produced from iPSCs of different origins
Biochimie. 2023 Dec 30:S0300-9084(23)00335-8. doi: 10.1016/j.biochi.2023.12.011. Online ahead of print.ABSTRACTDisease models based on induced pluripotent stem cells (iPSCs) are in high demand because of their physiological adequacy and well-reproducibility of the pathological phenotype. Nowadays, the most common approach to generate iPSCs is the reprogramming of somatic cells using vectors based on lentivirus or Sendai virus. We have previously shown impairments of calcium signaling including store-operated calcium entry in Huntington's disease-specific iPSCs-based GABA-ergic medium spiny neurons. However, different appro...
Source: Biochimie - January 1, 2024 Category: Biochemistry Authors: Arina A Oshkolova Dmitriy A Grekhnev Anna A Kruchinina Lilia D Belikova Egor A Volovikov Olga S Lebedeva Alexandra N Bogomazova Vladimir A Vigont Maria A Lagarkova Elena V Kaznacheyeva Source Type: research
Potential elevation of exopeptidase activity of Glu-specific endopeptidase I/GluV8 mediated by hydrophobic P1'-position amino acid residue
Biochimie. 2023 Dec 28:S0300-9084(23)00334-6. doi: 10.1016/j.biochi.2023.12.010. Online ahead of print.ABSTRACTWe recently reported that the activities of dipeptidyl-peptidase (DPP)7 and DPP11, S46-family exopeptidases were significantly elevated by the presence of prime-side amino acid residues of substrates caused by an increase in kcat [Ohara-Nemoto Y. et al., J Biol Chem 298(3):101585. doi: 10.1016/j.jbc.2022]. In the present study, the effects of prime-side residues on Glu-specific endopeptidase I/GluV8 from Staphylococcus aureus were investigated using a two-step cleavage method with tetrapeptidyl-methycoumaryl-7-ami...
Source: Biochimie - December 30, 2023 Category: Biochemistry Authors: Takayuki K Nemoto Haruka Nishimata Kana Shirakura Yuko Ohara-Nemoto Source Type: research
Naked mole-rat hyaluronan
Biochimie. 2023 Dec 27:S0300-9084(23)00332-2. doi: 10.1016/j.biochi.2023.12.008. Online ahead of print.ABSTRACTNaked mole rats (NMRs) are renowned for their exceptional longevity and remarkable maintenance of health throughout their lifetime. Their subterranean lifestyle has led to adaptations that have resulted in elevated levels of a very high molecular weight hyaluronan in their tissues. Hyaluronan, a glycosaminoglycan, is a key component of the extracellular matrix, which plays a critical role in maintaining tissue structure and regulating cell signaling pathways. This phenomenon in NMRs is attributed to a higher proce...
Source: Biochimie - December 29, 2023 Category: Biochemistry Authors: Francisco Alejandro Lagunas-Rangel Source Type: research
Purification and characterization of Cdr1, the drug-efflux pump conferring azole resistance in Candida species
Biochimie. 2023 Dec 27:S0300-9084(23)00331-0. doi: 10.1016/j.biochi.2023.12.007. Online ahead of print.ABSTRACTCandida albicans and C. glabrata express exporters of the ATP-binding cassette (ABC) superfamily and address them to their plasma membrane to expel azole antifungals, which cancels out their action and allows the yeast to become multidrug resistant (MDR). In a way to understand this mechanism of defense, we describe the purification and characterization of Cdr1, the membrane ABC exporter mainly responsible for such phenotype in both species. Cdr1 proteins were functionally expressed in the baker yeast, tagged at t...
Source: Biochimie - December 29, 2023 Category: Biochemistry Authors: Jorgaq Pata Alexis Moreno Benjamin Wiseman Sandrine Magnard Idriss Lehlali Marie Dujardin Atanu Banerjee Martin H ögbom Ahc ène Boumendjel Vincent Chaptal Rajendra Prasad Pierre Falson Source Type: research
Effects of osmolytes under crowding conditions on the properties of muscle glycogen phosphorylase b
Biochimie. 2023 Dec 20;220:48-57. doi: 10.1016/j.biochi.2023.12.005. Online ahead of print.ABSTRACTThe study of the relationship between the activity and stability of enzymes under crowding conditions in the presence of osmolytes is important for understanding the functioning of a living cell. The effect of osmolytes (trehalose and betaine) on the secondary and tertiary structure and activity of muscle glycogen phosphorylase b (Phb) under crowding conditions created by PEG 2000 and PEG 20000 was investigated using dynamic light scattering, differential scanning calorimetry, circular dichroism spectroscopy, fluorimetry and ...
Source: Biochimie - December 21, 2023 Category: Biochemistry Authors: Valeriya V Mikhaylova Tatiana B Eronina Source Type: research
Sensing of DNA modifications by pAgo proteins in vitro
Biochimie. 2023 Dec 19;220:39-47. doi: 10.1016/j.biochi.2023.12.006. Online ahead of print.ABSTRACTMany prokaryotic Argonaute (pAgo) proteins act as programmable nucleases that use small guide DNAs for recognition and cleavage of complementary target DNA. Recent studies suggested that pAgos participate in cell defense against invader DNA and may also be involved in other genetic processes, including DNA replication and repair. The ability of pAgos to recognize specific targets potentially make them an invaluable tool for DNA manipulations. Here, we demonstrate that DNA-guided DNA-targeting pAgo nucleases from three bacteri...
Source: Biochimie - December 21, 2023 Category: Biochemistry Authors: Margarita Beskrovnaia Aleksei Agapov Kristina Makasheva Dmitry O Zharkov Daria Esyunina Andrey Kulbachinskiy Source Type: research
Effects of osmolytes under crowding conditions on the properties of muscle glycogen phosphorylase b
Biochimie. 2023 Dec 20;220:48-57. doi: 10.1016/j.biochi.2023.12.005. Online ahead of print.ABSTRACTThe study of the relationship between the activity and stability of enzymes under crowding conditions in the presence of osmolytes is important for understanding the functioning of a living cell. The effect of osmolytes (trehalose and betaine) on the secondary and tertiary structure and activity of muscle glycogen phosphorylase b (Phb) under crowding conditions created by PEG 2000 and PEG 20000 was investigated using dynamic light scattering, differential scanning calorimetry, circular dichroism spectroscopy, fluorimetry and ...
Source: Biochimie - December 21, 2023 Category: Biochemistry Authors: Valeriya V Mikhaylova Tatiana B Eronina Source Type: research
Sensing of DNA modifications by pAgo proteins in vitro
Biochimie. 2023 Dec 19;220:39-47. doi: 10.1016/j.biochi.2023.12.006. Online ahead of print.ABSTRACTMany prokaryotic Argonaute (pAgo) proteins act as programmable nucleases that use small guide DNAs for recognition and cleavage of complementary target DNA. Recent studies suggested that pAgos participate in cell defense against invader DNA and may also be involved in other genetic processes, including DNA replication and repair. The ability of pAgos to recognize specific targets potentially make them an invaluable tool for DNA manipulations. Here, we demonstrate that DNA-guided DNA-targeting pAgo nucleases from three bacteri...
Source: Biochimie - December 21, 2023 Category: Biochemistry Authors: Margarita Beskrovnaia Aleksei Agapov Kristina Makasheva Dmitry O Zharkov Daria Esyunina Andrey Kulbachinskiy Source Type: research
Effects of osmolytes under crowding conditions on the properties of muscle glycogen phosphorylase b
Biochimie. 2023 Dec 20;220:48-57. doi: 10.1016/j.biochi.2023.12.005. Online ahead of print.ABSTRACTThe study of the relationship between the activity and stability of enzymes under crowding conditions in the presence of osmolytes is important for understanding the functioning of a living cell. The effect of osmolytes (trehalose and betaine) on the secondary and tertiary structure and activity of muscle glycogen phosphorylase b (Phb) under crowding conditions created by PEG 2000 and PEG 20000 was investigated using dynamic light scattering, differential scanning calorimetry, circular dichroism spectroscopy, fluorimetry and ...
Source: Biochimie - December 21, 2023 Category: Biochemistry Authors: Valeriya V Mikhaylova Tatiana B Eronina Source Type: research
