Impaired hepatic lipid metabolism and biomarkers in fatty liver diseases
Biochimie. 2023 Sep 26:S0300-9084(23)00247-X. doi: 10.1016/j.biochi.2023.09.020. Online ahead of print.ABSTRACTThe liver plays a crucial role in lipid metabolism and metabolic homeostasis. Non-Alcoholic Fatty Liver Disease (NAFLD) is the most common chronic liver disease worldwide and currently has no specific treatments. Lifestyle modifications such as weight loss, exercise, and dietary changes are recommended to reduce the risk factors associated with the disease. Oxidized cholesterol products, some phospholipids and diacylglycerols can activate inflammatory pathways and contribute to the progression to Non-Alcoholic Ste...
Source: Biochimie - September 28, 2023 Category: Biochemistry Authors: Francisca S Teixeira L ígia L Pimentel Manuela E Pintado Lu ís M Rodríguez-Alcalá Source Type: research
DNA damage-induced senescence is associated with metabolomic reprogramming in breast cancer cells
Biochimie. 2023 Sep 25:S0300-9084(23)00248-1. doi: 10.1016/j.biochi.2023.09.021. Online ahead of print.ABSTRACTSenescence due to exogenous and endogenous stresses triggers metabolic reprogramming and is associated with many pathologies, including cancer. In solid tumors, senescence promotes tumorigenesis, facilitates relapse, and changes the outcomes of anti-cancer therapies. Hence, cellular and molecular mechanisms regulating senescent pathways make attractive therapeutic targets. Cancer cells undergo metabolic reprogramming to sustain the growth-arrested state of senescence. In the present study, we aimed to understand t...
Source: Biochimie - September 27, 2023 Category: Biochemistry Authors: Neena George Manjunath B Joshi Kapaettu Satyamoorthy Source Type: research
Cathepsin C role in inflammatory gastroenterological, renal, rheumatic, and pulmonary disorders
Biochimie. 2023 Sep 25:S0300-9084(23)00245-6. doi: 10.1016/j.biochi.2023.09.018. Online ahead of print.ABSTRACTCathepsin C (CatC, syn. Dipeptidyl peptidase I) is a lysosomal cysteine proteinase expressed in several tissues including inflammatory cells. This enzyme is important for maintaining multiple cellular functions and for processing immune cell-derived proteases. While mutations in the CatC gene were reported in Papillon-Lefèvre syndrome, a rare autosomal recessive disorder featuring hyperkeratosis and periodontitis, evidence from clinical and preclinical studies points toward pro-inflammatory effects of CatC in var...
Source: Biochimie - September 27, 2023 Category: Biochemistry Authors: Ali A Aghdassi Christine Pham Lukas Zierke Vincent Mariaule Brice Korkmaz Moez Rhimi Source Type: research
DNA damage-induced senescence is associated with metabolomic reprogramming in breast cancer cells
Biochimie. 2023 Sep 25:S0300-9084(23)00248-1. doi: 10.1016/j.biochi.2023.09.021. Online ahead of print.ABSTRACTSenescence due to exogenous and endogenous stresses triggers metabolic reprogramming and is associated with many pathologies, including cancer. In solid tumors, senescence promotes tumorigenesis, facilitates relapse, and changes the outcomes of anti-cancer therapies. Hence, cellular and molecular mechanisms regulating senescent pathways make attractive therapeutic targets. Cancer cells undergo metabolic reprogramming to sustain the growth-arrested state of senescence. In the present study, we aimed to understand t...
Source: Biochimie - September 27, 2023 Category: Biochemistry Authors: Neena George Manjunath B Joshi Kapaettu Satyamoorthy Source Type: research
Cathepsin C role in inflammatory gastroenterological, renal, rheumatic, and pulmonary disorders
Biochimie. 2023 Sep 25:S0300-9084(23)00245-6. doi: 10.1016/j.biochi.2023.09.018. Online ahead of print.ABSTRACTCathepsin C (CatC, syn. Dipeptidyl peptidase I) is a lysosomal cysteine proteinase expressed in several tissues including inflammatory cells. This enzyme is important for maintaining multiple cellular functions and for processing immune cell-derived proteases. While mutations in the CatC gene were reported in Papillon-Lefèvre syndrome, a rare autosomal recessive disorder featuring hyperkeratosis and periodontitis, evidence from clinical and preclinical studies points toward pro-inflammatory effects of CatC in var...
Source: Biochimie - September 27, 2023 Category: Biochemistry Authors: Ali A Aghdassi Christine Pham Lukas Zierke Vincent Mariaule Brice Korkmaz Moez Rhimi Source Type: research
A Malassezia pseudoprotease dominates the secreted hydrolase landscape and is a potential allergen on skin
In this study we utilized targeted RNA sequencing from samples isolated directly from the skin to quantify gene expression of M. globosa secreted proteases, lipases, phospholipases and sphingomyelinases. Our findings indicate that the expression of these enzymes is dynamically regulated by the environment in which the fungus resides, as different growth phases of the planktonic culture of M. globosa show distinct expression levels. Furthermore, we observed significant differences in the expression of these enzymes in culture compared to healthy sebaceous skin sites. By examining the in situ gene expression of M. globosa's ...
Source: Biochimie - September 25, 2023 Category: Biochemistry Authors: Wisely Chua Carl O Marsh Si En Poh Winston Lc Koh Melody Li Ying Lee Li Fang Koh Xin-Zi Emily Tang Peter See Zheng Ser Shi Mei Wang Radoslaw M Sobota Thomas L Dawson Yik Weng Yew Steven Thng Anthony J O' Donoghue Hazel H Oon John E Common Hao Li Source Type: research
Last Year At Marienbad: Unusual Nucleic Acid structures
Biochimie. 2023 Sep 23:S0300-9084(23)00249-3. doi: 10.1016/j.biochi.2023.09.022. Online ahead of print.NO ABSTRACTPMID:37748749 | DOI:10.1016/j.biochi.2023.09.022 (Source: Biochimie)
Source: Biochimie - September 25, 2023 Category: Biochemistry Authors: Jean-Louis Mergny Luk áš Trantírek Giovanni Capranico Source Type: research
A Malassezia pseudoprotease dominates the secreted hydrolase landscape and is a potential allergen on skin
In this study we utilized targeted RNA sequencing from samples isolated directly from the skin to quantify gene expression of M. globosa secreted proteases, lipases, phospholipases and sphingomyelinases. Our findings indicate that the expression of these enzymes is dynamically regulated by the environment in which the fungus resides, as different growth phases of the planktonic culture of M. globosa show distinct expression levels. Furthermore, we observed significant differences in the expression of these enzymes in culture compared to healthy sebaceous skin sites. By examining the in situ gene expression of M. globosa's ...
Source: Biochimie - September 25, 2023 Category: Biochemistry Authors: Wisely Chua Carl O Marsh Si En Poh Winston Lc Koh Melody Li Ying Lee Li Fang Koh Xin-Zi Emily Tang Peter See Zheng Ser Shi Mei Wang Radoslaw M Sobota Thomas L Dawson Yik Weng Yew Steven Thng Anthony J O' Donoghue Hazel H Oon John E Common Hao Li Source Type: research
Last Year At Marienbad: Unusual Nucleic Acid structures
Biochimie. 2023 Sep 23:S0300-9084(23)00249-3. doi: 10.1016/j.biochi.2023.09.022. Online ahead of print.NO ABSTRACTPMID:37748749 | DOI:10.1016/j.biochi.2023.09.022 (Source: Biochimie)
Source: Biochimie - September 25, 2023 Category: Biochemistry Authors: Jean-Louis Mergny Luk áš Trantírek Giovanni Capranico Source Type: research
At the outer part of the active site in Trypanosoma cruzi glucokinase: The role of phenylalanine 337
In this study, F337 was changed to leucine and alanine so as to diminish phenylalanine's side chain size and attenuate intermolecular interactions in this region of the binding cavity. Results from enzyme - inhibitor assays revealed that the phenyl group of F337 made dominant hydrophobic interactions with the phenyl group of CBZ-GlcN as opposed to π - π stacking interactions. Moreover, enzymatic activity assays and X-ray crystallographic experiments indicated that each of these site-directed mutants primarily retained their activity and had high structural similarity of their protein fold. A computed structure model of T...
Source: Biochimie - September 23, 2023 Category: Biochemistry Authors: Shane M Carey Sean P Kearns Matthew E Millington Gregory S Buechner Beda E Alvarez Leily Daneshian Brendan Abiskaroon Maksymilian Chruszcz Edward L D'Antonio Source Type: research
The interplay between cis- and trans-acting factors drives selective mRNA translation initiation in eukaryotes
Biochimie. 2023 Sep 21:S0300-9084(23)00244-4. doi: 10.1016/j.biochi.2023.09.017. Online ahead of print.ABSTRACTTranslation initiation consists in the assembly of the small and large ribosomal subunits on the start codon. This important step directly modulates the general proteome in living cells. Recently, genome wide studies revealed unexpected translation initiation events from unsuspected novel open reading frames resulting in the synthesis of a so-called 'dark proteome'. Indeed, the identification of the start codon by the translation machinery is a critical step that defines the translational landscape of the cell. Th...
Source: Biochimie - September 23, 2023 Category: Biochemistry Authors: Antonin Tidu Franck Martin Source Type: research
At the outer part of the active site in Trypanosoma cruzi glucokinase: The role of phenylalanine 337
In this study, F337 was changed to leucine and alanine so as to diminish phenylalanine's side chain size and attenuate intermolecular interactions in this region of the binding cavity. Results from enzyme - inhibitor assays revealed that the phenyl group of F337 made dominant hydrophobic interactions with the phenyl group of CBZ-GlcN as opposed to π - π stacking interactions. Moreover, enzymatic activity assays and X-ray crystallographic experiments indicated that each of these site-directed mutants primarily retained their activity and had high structural similarity of their protein fold. A computed structure model of T...
Source: Biochimie - September 23, 2023 Category: Biochemistry Authors: Shane M Carey Sean P Kearns Matthew E Millington Gregory S Buechner Beda E Alvarez Leily Daneshian Brendan Abiskaroon Maksymilian Chruszcz Edward L D'Antonio Source Type: research
The interplay between cis- and trans-acting factors drives selective mRNA translation initiation in eukaryotes
Biochimie. 2023 Sep 21:S0300-9084(23)00244-4. doi: 10.1016/j.biochi.2023.09.017. Online ahead of print.ABSTRACTTranslation initiation consists in the assembly of the small and large ribosomal subunits on the start codon. This important step directly modulates the general proteome in living cells. Recently, genome wide studies revealed unexpected translation initiation events from unsuspected novel open reading frames resulting in the synthesis of a so-called 'dark proteome'. Indeed, the identification of the start codon by the translation machinery is a critical step that defines the translational landscape of the cell. Th...
Source: Biochimie - September 23, 2023 Category: Biochemistry Authors: Antonin Tidu Franck Martin Source Type: research
The emerging functions of mini zinc finger (MIF) microproteins in seed plants: A minireview
Biochimie. 2023 Sep 16:S0300-9084(23)00243-2. doi: 10.1016/j.biochi.2023.09.016. Online ahead of print.ABSTRACTMini zinc fingers constitute a class of microproteins that appeared early in evolution and expanded in seeds plants. In this review, the phylogenetic history, the functions and the mode of action of Mini zinc fingers in plants are reported and discussed. It appears that mini zinc fingers play an important role in the control of plant development. They are involved in the control of cell division and expansion, in the switch between the determinate/indeterminate state of the meristems and in the regulation of veget...
Source: Biochimie - September 18, 2023 Category: Biochemistry Authors: Marie Rose Ndella Thiaw Pascal Gantet Source Type: research
The emerging functions of mini zinc finger (MIF) microproteins in seed plants: A minireview
Biochimie. 2023 Sep 16:S0300-9084(23)00243-2. doi: 10.1016/j.biochi.2023.09.016. Online ahead of print.ABSTRACTMini zinc fingers constitute a class of microproteins that appeared early in evolution and expanded in seeds plants. In this review, the phylogenetic history, the functions and the mode of action of MIFs in plants are reported and discussed. It appears that mini zinc finger microproteins play an important role in the control of plant development. They are involved in the control of cell division and expansion and in the switch between the determinate/indeterminate state of the meristems. They are involved in the r...
Source: Biochimie - September 18, 2023 Category: Biochemistry Authors: Marie Rose Ndella Thiaw Pascal Gantet Source Type: research