Crosstalk between protein misfolding and endoplasmic reticulum stress during ageing and their role in age-related disorders
Biochimie. 2023 Oct 31:S0300-9084(23)00290-0. doi: 10.1016/j.biochi.2023.10.019. Online ahead of print.ABSTRACTMaintaining the proteome is crucial to retaining cell functionality and response to multiple intrinsic and extrinsic stressors. Protein misfolding increased the endoplasmic reticulum (ER) stress and activated the adaptive unfolded protein response (UPR) to restore cell homeostasis. Apoptosis occurs when ER stress is prolonged or the adaptive response fails. In healthy young cells, the ratio of protein folding machinery to quantities of misfolded proteins is balanced under normal circumstances. However, the age-rel...
Source: Biochimie - November 2, 2023 Category: Biochemistry Authors: Manisekaran Hemagirri Yeng Chen Subash C B Gopinath Sumaira Sahreen Mohd Adnan Sreenivasan Sasidharan Source Type: research

Crosstalk between protein misfolding and endoplasmic reticulum stress during ageing and their role in age-related disorders
Biochimie. 2023 Oct 31:S0300-9084(23)00290-0. doi: 10.1016/j.biochi.2023.10.019. Online ahead of print.ABSTRACTMaintaining the proteome is crucial to retaining cell functionality and response to multiple intrinsic and extrinsic stressors. Protein misfolding increased the endoplasmic reticulum (ER) stress and activated the adaptive unfolded protein response (UPR) to restore cell homeostasis. Apoptosis occurs when ER stress is prolonged or the adaptive response fails. In healthy young cells, the ratio of protein folding machinery to quantities of misfolded proteins is balanced under normal circumstances. However, the age-rel...
Source: Biochimie - November 2, 2023 Category: Biochemistry Authors: Manisekaran Hemagirri Yeng Chen Subash C B Gopinath Sumaira Sahreen Mohd Adnan Sreenivasan Sasidharan Source Type: research

Venom of the neotropical rattlesnake, Crotalus culminatus: Intraspecific variation, neutralization by antivenoms, and immunogenicity in rabbits
In this study, we aimed to compare the biochemical and biological activities of the venom of juvenile and adult snakes. Additionally, we assessed antibody production using the venoms of juveniles and adults as immunogens in rabbits. Our findings reveal lethality and proteolytic activity differences between the venoms of juveniles and adults. Notably, juvenile venoms exhibited high proportions of crotamine, while adult venoms displayed a reduction of this component. A commercially available antivenom demonstrated effective neutralization of lethality of both juvenile and adult venoms in mice. However, it failed to neutraliz...
Source: Biochimie - October 27, 2023 Category: Biochemistry Authors: Vanessa Zarzosa Bruno Lomonte Fernando Zamudio Roberto Ponce-L ópez Felipe Olvera-Rodr íguez Miguel Borja Alejandro Alag ón Edgar Neri-Castro Source Type: research

Venom of the neotropical rattlesnake, Crotalus culminatus: Intraspecific variation, neutralization by antivenoms, and immunogenicity in rabbits
In this study, we aimed to compare the biochemical and biological activities of the venom of juvenile and adult snakes. Additionally, we assessed antibody production using the venoms of juveniles and adults as immunogens in rabbits. Our findings reveal lethality and proteolytic activity differences between the venoms of juveniles and adults. Notably, juvenile venoms exhibited high proportions of crotamine, while adult venoms displayed a reduction of this component. A commercially available antivenom demonstrated effective neutralization of lethality of both juvenile and adult venoms in mice. However, it failed to neutraliz...
Source: Biochimie - October 27, 2023 Category: Biochemistry Authors: Vanessa Zarzosa Bruno Lomonte Fernando Zamudio Roberto Ponce-L ópez Felipe Olvera-Rodr íguez Miguel Borja Alejandro Alag ón Edgar Neri-Castro Source Type: research

Venom of the neotropical rattlesnake, Crotalus culminatus: Intraspecific variation, neutralization by antivenoms, and immunogenicity in rabbits
In this study, we aimed to compare the biochemical and biological activities of the venom of juvenile and adult snakes. Additionally, we assessed antibody production using the venoms of juveniles and adults as immunogens in rabbits. Our findings reveal lethality and proteolytic activity differences between the venoms of juveniles and adults. Notably, juvenile venoms exhibited high proportions of crotamine, while adult venoms displayed a reduction of this component. A commercially available antivenom demonstrated effective neutralization of lethality of both juvenile and adult venoms in mice. However, it failed to neutraliz...
Source: Biochimie - October 27, 2023 Category: Biochemistry Authors: Vanessa Zarzosa Bruno Lomonte Fernando Zamudio Roberto Ponce-L ópez Felipe Olvera-Rodr íguez Miguel Borja Alejandro Alag ón Edgar Neri-Castro Source Type: research

Venom of the neotropical rattlesnake, Crotalus culminatus: Intraspecific variation, neutralization by antivenoms, and immunogenicity in rabbits
In this study, we aimed to compare the biochemical and biological activities of the venom of juvenile and adult snakes. Additionally, we assessed antibody production using the venoms of juveniles and adults as immunogens in rabbits. Our findings reveal lethality and proteolytic activity differences between the venoms of juveniles and adults. Notably, juvenile venoms exhibited high proportions of crotamine, while adult venoms displayed a reduction of this component. A commercially available antivenom demonstrated effective neutralization of lethality of both juvenile and adult venoms in mice. However, it failed to neutraliz...
Source: Biochimie - October 27, 2023 Category: Biochemistry Authors: Vanessa Zarzosa Bruno Lomonte Fernando Zamudio Roberto Ponce-L ópez Felipe Olvera-Rodr íguez Miguel Borja Alejandro Alag ón Edgar Neri-Castro Source Type: research

Venom of the neotropical rattlesnake, Crotalus culminatus: Intraspecific variation, neutralization by antivenoms, and immunogenicity in rabbits
In this study, we aimed to compare the biochemical and biological activities of the venom of juvenile and adult snakes. Additionally, we assessed antibody production using the venoms of juveniles and adults as immunogens in rabbits. Our findings reveal lethality and proteolytic activity differences between the venoms of juveniles and adults. Notably, juvenile venoms exhibited high proportions of crotamine, while adult venoms displayed a reduction of this component. A commercially available antivenom demonstrated effective neutralization of lethality of both juvenile and adult venoms in mice. However, it failed to neutraliz...
Source: Biochimie - October 27, 2023 Category: Biochemistry Authors: Vanessa Zarzosa Bruno Lomonte Fernando Zamudio Roberto Ponce-L ópez Felipe Olvera-Rodr íguez Miguel Borja Alejandro Alag ón Edgar Neri-Castro Source Type: research

Cytotoxic effects of Chartergellus communis wasp venom peptide against melanoma cells
Biochimie. 2023 Oct 23:S0300-9084(23)00278-X. doi: 10.1016/j.biochi.2023.10.015. Online ahead of print.ABSTRACTCancer is a huge public health problem being one of the main causes of death globally. Specifically, melanoma is one of the most threatening cancer types due to the metastatic capacity, treatment resistance and mortality rates. It is evident the urgent need for research on new agents with pharmacological potential for cancer treatment, in order to develop new cancer therapeutic strategies and overcome drug resistance. The present work investigated the anti-tumoral potential of Chartergellus-CP1 peptide, isolated f...
Source: Biochimie - October 25, 2023 Category: Biochemistry Authors: Ver ónica Bastos Simone Pascoal Kamila Lopes M árcia Mortari Helena Oliveira Source Type: research

ESX-3 secretion system in Mycobacterium: An overview
Biochimie. 2023 Oct 23:S0300-9084(23)00276-6. doi: 10.1016/j.biochi.2023.10.013. Online ahead of print.ABSTRACTMycobacteria are microorganisms distributed in the environment worldwide, and some of them, such as Mycobacterium tuberculosis or M. leprae, are pathogenic. The hydrophobic mycobacterial cell envelope has low permeation and bacteria need to export products across their structure. Mycobacteria possess specialized protein secretion systems, such as the Early Secretory Antigenic Target 6 secretion (ESX) system. Five ESX loci have been described in M. tuberculosis, called ESX1 to ESX5. The ESX3 secretion system has be...
Source: Biochimie - October 25, 2023 Category: Biochemistry Authors: Ana Laura Granados-Trist án Carlos Eduardo Hern ández-Luna Laura Adiene Gonz ález-Escalante Mar ía Elena Camacho-Moll Beatriz Silva-Ram írez Mario Berm údez de León Katia Pe ñuelas-Urquides Source Type: research

Cytotoxic effects of Chartergellus communis wasp venom peptide against melanoma cells
Biochimie. 2023 Oct 23:S0300-9084(23)00278-X. doi: 10.1016/j.biochi.2023.10.015. Online ahead of print.ABSTRACTCancer is a huge public health problem being one of the main causes of death globally. Specifically, melanoma is one of the most threatening cancer types due to the metastatic capacity, treatment resistance and mortality rates. It is evident the urgent need for research on new agents with pharmacological potential for cancer treatment, in order to develop new cancer therapeutic strategies and overcome drug resistance. The present work investigated the anti-tumoral potential of Chartergellus-CP1 peptide, isolated f...
Source: Biochimie - October 25, 2023 Category: Biochemistry Authors: Ver ónica Bastos Simone Pascoal Kamila Lopes M árcia Mortari Helena Oliveira Source Type: research

ESX-3 secretion system in Mycobacterium: An overview
Biochimie. 2023 Oct 23:S0300-9084(23)00276-6. doi: 10.1016/j.biochi.2023.10.013. Online ahead of print.ABSTRACTMycobacteria are microorganisms distributed in the environment worldwide, and some of them, such as Mycobacterium tuberculosis or M. leprae, are pathogenic. The hydrophobic mycobacterial cell envelope has low permeation and bacteria need to export products across their structure. Mycobacteria possess specialized protein secretion systems, such as the Early Secretory Antigenic Target 6 secretion (ESX) system. Five ESX loci have been described in M. tuberculosis, called ESX1 to ESX5. The ESX3 secretion system has be...
Source: Biochimie - October 25, 2023 Category: Biochemistry Authors: Ana Laura Granados-Trist án Carlos Eduardo Hern ández-Luna Laura Adiene Gonz ález-Escalante Mar ía Elena Camacho-Moll Beatriz Silva-Ram írez Mario Berm údez de León Katia Pe ñuelas-Urquides Source Type: research

Cytotoxic effects of Chartergellus communis wasp venom peptide against melanoma cells
Biochimie. 2023 Oct 23:S0300-9084(23)00278-X. doi: 10.1016/j.biochi.2023.10.015. Online ahead of print.ABSTRACTCancer is a huge public health problem being one of the main causes of death globally. Specifically, melanoma is one of the most threatening cancer types due to the metastatic capacity, treatment resistance and mortality rates. It is evident the urgent need for research on new agents with pharmacological potential for cancer treatment, in order to develop new cancer therapeutic strategies and overcome drug resistance. The present work investigated the anti-tumoral potential of Chartergellus-CP1 peptide, isolated f...
Source: Biochimie - October 25, 2023 Category: Biochemistry Authors: Ver ónica Bastos Simone Pascoal Kamila Lopes M árcia Mortari Helena Oliveira Source Type: research

ESX-3 secretion system in Mycobacterium: An overview
Biochimie. 2023 Oct 23:S0300-9084(23)00276-6. doi: 10.1016/j.biochi.2023.10.013. Online ahead of print.ABSTRACTMycobacteria are microorganisms distributed in the environment worldwide, and some of them, such as Mycobacterium tuberculosis or M. leprae, are pathogenic. The hydrophobic mycobacterial cell envelope has low permeation and bacteria need to export products across their structure. Mycobacteria possess specialized protein secretion systems, such as the Early Secretory Antigenic Target 6 secretion (ESX) system. Five ESX loci have been described in M. tuberculosis, called ESX1 to ESX5. The ESX3 secretion system has be...
Source: Biochimie - October 25, 2023 Category: Biochemistry Authors: Ana Laura Granados-Trist án Carlos Eduardo Hern ández-Luna Laura Adiene Gonz ález-Escalante Mar ía Elena Camacho-Moll Beatriz Silva-Ram írez Mario Berm údez de León Katia Pe ñuelas-Urquides Source Type: research

Anti-smoking drugs cytisine and varenicline reduce cardiac reperfusion injury in rat model of myocardial ischemia
Biochimie. 2023 Oct 21:S0300-9084(23)00274-2. doi: 10.1016/j.biochi.2023.10.011. Online ahead of print.ABSTRACTEvidence to date indicates that activation of nicotinic acetylcholine receptors (nAChRs) can reduce cardiac injury from ischemia and subsequent reperfusion. The use of nAChR agonists in various animal models leads to a reduction in reperfusion injury. Earlier this effect was shown for the agonists of α7 nAChR subtype. In this work, we demonstrated the expression of mRNA encoding α4, α6 and β2 nAChR subunits in the left ventricle of rat heart. In a rat model of myocardial ischemia, we studied the effect of α4Î...
Source: Biochimie - October 23, 2023 Category: Biochemistry Authors: Elvira R Shaykhutdinova Maria S Severyukhina Inna V Kholoshenko Elena A Gondarenko Irina V Shelukhina Elena V Kryukova Alina M Ismailova Elena S Sadovnikova Igor A Dyachenko Arkady N Murashev Victor I Tsetlin Yuri N Utkin Source Type: research

Anti-smoking drugs cytisine and varenicline reduce cardiac reperfusion injury in rat model of myocardial ischemia
Biochimie. 2023 Oct 21:S0300-9084(23)00274-2. doi: 10.1016/j.biochi.2023.10.011. Online ahead of print.ABSTRACTEvidence to date indicates that activation of nicotinic acetylcholine receptors (nAChRs) can reduce cardiac injury from ischemia and subsequent reperfusion. The use of nAChR agonists in various animal models leads to a reduction in reperfusion injury. Earlier this effect was shown for the agonists of α7 nAChR subtype. In this work, we demonstrated the expression of mRNA encoding α4, α6 and β2 nAChR subunits in the left ventricle of rat heart. In a rat model of myocardial ischemia, we studied the effect of α4Î...
Source: Biochimie - October 23, 2023 Category: Biochemistry Authors: Elvira R Shaykhutdinova Maria S Severyukhina Inna V Kholoshenko Elena A Gondarenko Irina V Shelukhina Elena V Kryukova Alina M Ismailova Elena S Sadovnikova Igor A Dyachenko Arkady N Murashev Victor I Tsetlin Yuri N Utkin Source Type: research