Transcriptional regulation of the Japanese flounder Cu,Zn-SOD (Jfsod1) gene in RAW264.7 cells during oxidative stress caused by causative bacteria of edwardsiellosis
In this study, NUF251 stimulated intracellular superoxide radical production in mouse macrophage RAW264.7 cells, which was reduced by N-acetylcysteine. This result suggests that NUF251 infection causes oxidative stress. To evaluate the regulatory mechanism of Jfsod1 at transcriptional levels under oxidative stress induced by NUF251 infection, we cloned and determined the nucleotide sequence (1124 bp) of the 5'-flanking region of the Jfsod1 gene. The sequence analysis demonstrated that the binding sites for the transcription factors C/EBPα and NF-IL6 involved in the transcriptional regulation of the mammalian sod1 gene exi...
Source: Biochimie - September 4, 2023 Category: Biochemistry Authors: Xiao-Mi Sun Asami Yoshida Takuya Ishii Yan-Rong Jiang Yi-Li Gao Mikinori Ueno Katsuya Hirasaka Kiyoshi Osatomi Source Type: research
Small molecule chaperones facilitate the folding of RNA G-quadruplexes
Biochimie. 2023 Sep 2:S0300-9084(23)00207-9. doi: 10.1016/j.biochi.2023.08.016. Online ahead of print.ABSTRACTRNA G-quadruplexes (rG4) have recently emerged as major regulatory elements in both mRNA and non-coding RNA. In order to investigate the biological roles of rG4 structures, chemists have developed a variety of highly specific and potent ligands. All of these ligands bind to the rG4s by stacking on top of them. The binding specificity is demonstrated by comparison to other structures such as duplex or three-way junctions. It remains unclear whether rG4-ligands merely stabilize fully formed rG4 structures, or if they...
Source: Biochimie - September 4, 2023 Category: Biochemistry Authors: Pauline Lejault Louis Prudent Michel-Pierre Terrier Jean-Pierre Perreault Source Type: research
Transcriptional regulation of the Japanese flounder Cu,Zn-SOD (Jfsod1) gene in RAW264.7 cells during oxidative stress caused by causative bacteria of edwardsiellosis
In this study, NUF251 stimulated intracellular superoxide radical production in mouse macrophage RAW264.7 cells, which was reduced by N-acetylcysteine. This result suggests that NUF251 infection causes oxidative stress. To evaluate the regulatory mechanism of Jfsod1 at transcriptional levels under oxidative stress induced by NUF251 infection, we cloned and determined the nucleotide sequence (1124 bp) of the 5'-flanking region of the Jfsod1 gene. The sequence analysis demonstrated that the binding sites for the transcription factors C/EBPα and NF-IL6 involved in the transcriptional regulation of the mammalian sod1 gene exi...
Source: Biochimie - September 4, 2023 Category: Biochemistry Authors: Xiao-Mi Sun Asami Yoshida Takuya Ishii Yan-Rong Jiang Yi-Li Gao Mikinori Ueno Katsuya Hirasaka Kiyoshi Osatomi Source Type: research
Small molecule chaperones facilitate the folding of RNA G-quadruplexes
Biochimie. 2023 Sep 2:S0300-9084(23)00207-9. doi: 10.1016/j.biochi.2023.08.016. Online ahead of print.ABSTRACTRNA G-quadruplexes (rG4) have recently emerged as major regulatory elements in both mRNA and non-coding RNA. In order to investigate the biological roles of rG4 structures, chemists have developed a variety of highly specific and potent ligands. All of these ligands bind to the rG4s by stacking on top of them. The binding specificity is demonstrated by comparison to other structures such as duplex or three-way junctions. It remains unclear whether rG4-ligands merely stabilize fully formed rG4 structures, or if they...
Source: Biochimie - September 4, 2023 Category: Biochemistry Authors: Pauline Lejault Louis Prudent Michel-Pierre Terrier Jean-Pierre Perreault Source Type: research
Transcriptional regulation of the Japanese flounder Cu,Zn-SOD (Jfsod1) gene in RAW264.7 cells during oxidative stress caused by causative bacteria of edwardsiellosis
In this study, NUF251 stimulated intracellular superoxide radical production in mouse macrophage RAW264.7 cells, which was reduced by N-acetylcysteine. This result suggests that NUF251 infection causes oxidative stress. To evaluate the regulatory mechanism of Jfsod1 at transcriptional levels under oxidative stress induced by NUF251 infection, we cloned and determined the nucleotide sequence (1124 bp) of the 5'-flanking region of the Jfsod1 gene. The sequence analysis demonstrated that the binding sites for the transcription factors C/EBPα and NF-IL6 involved in the transcriptional regulation of the mammalian sod1 gene exi...
Source: Biochimie - September 4, 2023 Category: Biochemistry Authors: Xiao-Mi Sun Asami Yoshida Takuya Ishii Yan-Rong Jiang Yi-Li Gao Mikinori Ueno Katsuya Hirasaka Kiyoshi Osatomi Source Type: research
G-quadruplex forming sequences in the genes coding for cytochrome P450 enzymes and their potential roles in drug metabolism
In conclusion, our findings indicate the potential role of G-quadruplexes in cytochrome genes regulation, and emphasize the importance of G-quadruplexes in drug metabolism.PMID:37660977 | DOI:10.1016/j.biochi.2023.08.014 (Source: Biochimie)
Source: Biochimie - September 3, 2023 Category: Biochemistry Authors: Mona Saad Rongxin Zhang Anne Cucchiarini Cybel Mehawej Jean-Louis Mergny Mohamad Mroueh Wissam H Faour Source Type: research
Structural studies with crotoxin B from Crotalus durissus collilineatus venom suggest a heterodimeric assembly formed by two new isoforms
Biochimie. 2023 Aug 31:S0300-9084(23)00209-2. doi: 10.1016/j.biochi.2023.08.018. Online ahead of print.ABSTRACTIn accidents involving Crotalus snakes, the crotoxin complex (CTX) plays lethal action due to its neurotoxic activity. On the other hand, CTX have potential biotechnological application due to its anti-tumoral, anti-inflammatory, antimicrobial, analgesic and immunomodulatory properties. CTX is a heterodimer composed of Crotoxin A (CA or crotapotin), the acidic nontoxic and non-enzymatic component and; Crotoxin B (CB), a basic, toxic and catalytic PLA2. Currently, there are two classes of CTX isoforms, whose differ...
Source: Biochimie - September 2, 2023 Category: Biochemistry Authors: Guilherme H M Salvador Carlos A H Fernandes Rafael J Borges Andreimar M Soares Marcos R M Fontes Source Type: research
Structural studies with crotoxin B from Crotalus durissus collilineatus venom suggest a heterodimeric assembly formed by two new isoforms
Biochimie. 2023 Aug 31:S0300-9084(23)00209-2. doi: 10.1016/j.biochi.2023.08.018. Online ahead of print.ABSTRACTIn accidents involving Crotalus snakes, the crotoxin complex (CTX) plays lethal action due to its neurotoxic activity. On the other hand, CTX have potential biotechnological application due to its anti-tumoral, anti-inflammatory, antimicrobial, analgesic and immunomodulatory properties. CTX is a heterodimer composed of Crotoxin A (CA or crotapotin), the acidic nontoxic and non-enzymatic component and; Crotoxin B (CB), a basic, toxic and catalytic PLA2. Currently, there are two classes of CTX isoforms, whose differ...
Source: Biochimie - September 2, 2023 Category: Biochemistry Authors: Guilherme H M Salvador Carlos A H Fernandes Rafael J Borges Andreimar M Soares Marcos R M Fontes Source Type: research
Translational control by long non-coding RNAs
Biochimie. 2023 Aug 26:S0300-9084(23)00206-7. doi: 10.1016/j.biochi.2023.08.015. Online ahead of print.ABSTRACTLong non-coding (lnc) RNAs, once considered as junk and useless, are now broadly recognized to have major functions in the cell. LncRNAs are defined as non-coding RNAs of more than 200 nucleotides, regulate all steps of gene expression. Their origin is diverse, they can arise from intronic, intergenic or overlapping region, in sense or antisense direction. LncRNAs are mainly described for their action on transcription, while their action at the translational level is more rarely cited. However, the bibliography in...
Source: Biochimie - August 28, 2023 Category: Biochemistry Authors: Anne-Claire Godet Emilie Roussel Nathalie Laugero Florent Morfoisse Eric Lacazette Barbara Garmy-Susini Anne-Catherine Prats Source Type: research
Translational control by long non-coding RNAs
Biochimie. 2023 Aug 26:S0300-9084(23)00206-7. doi: 10.1016/j.biochi.2023.08.015. Online ahead of print.ABSTRACTLong non-coding (lnc) RNAs, once considered as junk and useless, are now broadly recognized to have major functions in the cell. LncRNAs are defined as non-coding RNAs of more than 200 nucleotides, regulate all steps of gene expression. Their origin is diverse, they can arise from intronic, intergenic or overlapping region, in sense or antisense direction. LncRNAs are mainly described for their action on transcription, while their action at the translational level is more rarely cited. However, the bibliography in...
Source: Biochimie - August 28, 2023 Category: Biochemistry Authors: Anne-Claire Godet Emilie Roussel Nathalie Laugero Florent Morfoisse Eric Lacazette Barbara Garmy-Susini Anne-Catherine Prats Source Type: research
Translational control by long non-coding RNAs
Biochimie. 2023 Aug 26:S0300-9084(23)00206-7. doi: 10.1016/j.biochi.2023.08.015. Online ahead of print.ABSTRACTLong non-coding (lnc) RNAs, once considered as junk and useless, are now broadly recognized to have major functions in the cell. LncRNAs are defined as non-coding RNAs of more than 200 nucleotides, regulate all steps of gene expression. Their origin is diverse, they can arise from intronic, intergenic or overlapping region, in sense or antisense direction. LncRNAs are mainly described for their action on transcription, while their action at the translational level is more rarely cited. However, the bibliography in...
Source: Biochimie - August 28, 2023 Category: Biochemistry Authors: Anne-Claire Godet Emilie Roussel Nathalie Laugero Florent Morfoisse Eric Lacazette Barbara Garmy-Susini Anne-Catherine Prats Source Type: research
Translational control by long non-coding RNAs
Biochimie. 2023 Aug 26:S0300-9084(23)00206-7. doi: 10.1016/j.biochi.2023.08.015. Online ahead of print.ABSTRACTLong non-coding (lnc) RNAs, once considered as junk and useless, are now broadly recognized to have major functions in the cell. LncRNAs are defined as non-coding RNAs of more than 200 nucleotides, regulate all steps of gene expression. Their origin is diverse, they can arise from intronic, intergenic or overlapping region, in sense or antisense direction. LncRNAs are mainly described for their action on transcription, while their action at the translational level is more rarely cited. However, the bibliography in...
Source: Biochimie - August 28, 2023 Category: Biochemistry Authors: Anne-Claire Godet Emilie Roussel Nathalie Laugero Florent Morfoisse Eric Lacazette Barbara Garmy-Susini Anne-Catherine Prats Source Type: research
Translational control by long non-coding RNAs
Biochimie. 2023 Aug 26:S0300-9084(23)00206-7. doi: 10.1016/j.biochi.2023.08.015. Online ahead of print.ABSTRACTLong non-coding (lnc) RNAs, once considered as junk and useless, are now broadly recognized to have major functions in the cell. LncRNAs are defined as non-coding RNAs of more than 200 nucleotides, regulate all steps of gene expression. Their origin is diverse, they can arise from intronic, intergenic or overlapping region, in sense or antisense direction. LncRNAs are mainly described for their action on transcription, while their action at the translational level is more rarely cited. However, the bibliography in...
Source: Biochimie - August 28, 2023 Category: Biochemistry Authors: Anne-Claire Godet Emilie Roussel Nathalie Laugero Florent Morfoisse Eric Lacazette Barbara Garmy-Susini Anne-Catherine Prats Source Type: research
PTEN regulates expression of its pseudogene in glioblastoma cells in DNA methylation-dependent manner
Biochimie. 2023 Aug 22:S0300-9084(23)00201-8. doi: 10.1016/j.biochi.2023.08.010. Online ahead of print.ABSTRACTGlioblastoma (GBM) is the most aggressive and frequent type of primary brain cancer in adult patients. One of the key molecular features associated with GBM pathogenesis is the dysfunction of PTEN oncosuppressor. In addition to PTEN gene, humans and several primates possess processed PTEN pseudogene (PTENP1) that gives rise to long non-coding RNA lncPTENP1-S. Regulation and functions of PTEN and PTENP1 are highly interconnected, however, the exact molecular mechanism of how these two genes affect each other remain...
Source: Biochimie - August 24, 2023 Category: Biochemistry Authors: Tatyana F Kovalenko Bhupender Yadav Ksenia S Anufrieva Tatyana D Larionova Tatiana E Aksinina Yaroslav A Latyshev Soniya Bastola Michail I Shakhparonov Amit Kumar Pandey Marat S Pavlyukov Source Type: research
Regulation and function of R-loops at repetitive elements
Biochimie. 2023 Aug 22:S0300-9084(23)00204-3. doi: 10.1016/j.biochi.2023.08.013. Online ahead of print.ABSTRACTR-loops are atypical, three-stranded nucleic acid structures that contain a stretch of RNA:DNA hybrids and an unpaired, single stranded DNA loop. R-loops are physiological relevant and can act as regulators of gene expression, chromatin structure, DNA damage repair and DNA replication. However, unscheduled and persistent R-loops are mutagenic and can mediate replication-transcription conflicts, leading to DNA damage and genome instability if left unchecked. Detailed transcriptome analysis unveiled that 85% of the ...
Source: Biochimie - August 24, 2023 Category: Biochemistry Authors: Alice Gambelli Alessandro Ferrando Chiara Boncristiani Stefan Schoeftner Source Type: research