Alpha hemolysin of E. coli induces hemolysis of human erythrocytes independently of toxin interaction with membrane proteins
Biochimie. 2023 Oct 9:S0300-9084(23)00271-7. doi: 10.1016/j.biochi.2023.10.008. Online ahead of print.ABSTRACTAlpha hemolysin (HlyA) is a hemolytic and cytotoxic protein secreted by uropathogenic strains of E. coli. The role of glycophorins (GPs) as putative receptors for HlyA binding to red blood cells (RBCs) has been debated. Experiments using anti-GPA/GPB antibodies and a GPA-specific epitope nanobody to block HlyA-GP binding on hRBCs, showed no effect on hemolytic activity. Similarly, the hemolysis induced by HlyA remained unaffected when hRBCs from a GPAnull/GPBnull variant were used. Surface Plasmon Resonance experim...
Source: Biochimie - October 11, 2023 Category: Biochemistry Authors: Luc ía Cané Nicol ás Andrés Saffioti Sandrine Genetet Mar ía Antonieta Daza Millone Mariano A Ostuni Pablo J Schwarzbaum Isabelle Mouro-Chanteloup Vanesa Herlax Source Type: research
Structure and function of Campylobacter jejuni polynucleotide phosphorylase (PNPase): Insights into the role of this RNase in pathogenicity
Biochimie. 2023 Oct 6:S0300-9084(23)00268-7. doi: 10.1016/j.biochi.2023.10.006. Online ahead of print.ABSTRACTRibonucleases are in charge of the processing, degradation and quality control of all cellular transcripts, which makes them crucial factors in RNA regulation. This post-transcriptional regulation allows bacteria to promptly react to different stress conditions and growth phase transitions, and also to produce the required virulence factors in pathogenic bacteria. Campylobacter jejuni is the main responsible for human gastroenteritis in the world. In this foodborne pathogen, exoribonuclease PNPase (CjPNP) is essent...
Source: Biochimie - October 8, 2023 Category: Biochemistry Authors: C átia Bárria Diogo Athayde Guillem Hernandez Leonor Fonseca Jorge Casinhas Tiago N Cordeiro Margarida Archer Cec ília M Arraiano Jos é A Brito Rute G Matos Source Type: research
A U2 snRNP-specific protein, U2A', is involved in stress response and drug resistance in Cryptococcus deneoformans
In this study, we identified a U2A' homologous protein as a component of the cryptococcal U2 snRNP, which was encoded by the LEA1 gene. Using the "suicide" CRISPR-Cas9 tool, we deleted the LEA1 gene in C. deneoformans JEC21 strain and obtained the disruption mutant lea1Δ. The mutant showed a hypersensitivity to 0.03 % sodium dodecyl sulfate, as well as disordered chitin distribution in cell wall observed with Calcofluor White staining, which collectively illustrated the function of U2A' in maintenance of cell wall integrity. Further examination showed that lea1Δ displayed a decreased tolerance to lower or elevated temper...
Source: Biochimie - October 8, 2023 Category: Biochemistry Authors: Lan Ma Xueqing Zhang Chenxi Li Xiaoyu Ma Xuan Zhao Xueru Zhao Ping Zhang Xudong Zhu Source Type: research
Coordination between human DNA polymerase β and apurinic/apyrimidinic endonuclease 1 in the course of DNA repair
Biochimie. 2023 Oct 6:S0300-9084(23)00270-5. doi: 10.1016/j.biochi.2023.10.007. Online ahead of print.ABSTRACTCoordination of enzymatic activities in the course of base excision repair (BER) is essential to ensure complete repair of damaged bases. Two major mechanisms underlying the coordination of BER are known today: the "passing the baton" model and a model of preassembled stable multiprotein repair complexes called "repairosomes." In this work, we aimed to elucidate the coordination between human apurinic/apyrimidinic (AP) endonuclease APE1 and DNA polymerase Polβ in BER through studying an impact of APE1 on Polβ-cat...
Source: Biochimie - October 8, 2023 Category: Biochemistry Authors: Artemiy S Bakman Stanislav S Boichenko Aleksandra A Kuznetsova Alexander A Ishchenko Murat Saparbaev Nikita A Kuznetsov Source Type: research
Structure and function of Campylobacter jejuni polynucleotide phosphorylase (PNPase): Insights into the role of this RNase in pathogenicity
Biochimie. 2023 Oct 6:S0300-9084(23)00268-7. doi: 10.1016/j.biochi.2023.10.006. Online ahead of print.ABSTRACTRibonucleases are in charge of the processing, degradation and quality control of all cellular transcripts, which makes them crucial factors in RNA regulation. This post-transcriptional regulation allows bacteria to promptly react to different stress conditions and growth phase transitions, and also to produce the required virulence factors in pathogenic bacteria. Campylobacter jejuni is the main responsible for human gastroenteritis in the world. In this foodborne pathogen, exoribonuclease PNPase (CjPNP) is essent...
Source: Biochimie - October 8, 2023 Category: Biochemistry Authors: C átia Bárria Diogo Athayde Guillem Hernandez Leonor Fonseca Jorge Casinhas Tiago N Cordeiro Margarida Archer Cec ília M Arraiano Jos é A Brito Rute G Matos Source Type: research
A U2 snRNP-specific protein, U2A', is involved in stress response and drug resistance in Cryptococcus deneoformans
In this study, we identified a U2A' homologous protein as a component of the cryptococcal U2 snRNP, which was encoded by the LEA1 gene. Using the "suicide" CRISPR-Cas9 tool, we deleted the LEA1 gene in C. deneoformans JEC21 strain and obtained the disruption mutant lea1Δ. The mutant showed a hypersensitivity to 0.03 % sodium dodecyl sulfate, as well as disordered chitin distribution in cell wall observed with Calcofluor White staining, which collectively illustrated the function of U2A' in maintenance of cell wall integrity. Further examination showed that lea1Δ displayed a decreased tolerance to lower or elevated temper...
Source: Biochimie - October 8, 2023 Category: Biochemistry Authors: Lan Ma Xueqing Zhang Chenxi Li Xiaoyu Ma Xuan Zhao Xueru Zhao Ping Zhang Xudong Zhu Source Type: research
Coordination between human DNA polymerase β and apurinic/apyrimidinic endonuclease 1 in the course of DNA repair
Biochimie. 2023 Oct 6:S0300-9084(23)00270-5. doi: 10.1016/j.biochi.2023.10.007. Online ahead of print.ABSTRACTCoordination of enzymatic activities in the course of base excision repair (BER) is essential to ensure complete repair of damaged bases. Two major mechanisms underlying the coordination of BER are known today: the "passing the baton" model and a model of preassembled stable multiprotein repair complexes called "repairosomes." In this work, we aimed to elucidate the coordination between human apurinic/apyrimidinic (AP) endonuclease APE1 and DNA polymerase Polβ in BER through studying an impact of APE1 on Polβ-cat...
Source: Biochimie - October 8, 2023 Category: Biochemistry Authors: Artemiy S Bakman Stanislav S Boichenko Aleksandra A Kuznetsova Alexander A Ishchenko Murat Saparbaev Nikita A Kuznetsov Source Type: research
Structure and function of Campylobacter jejuni polynucleotide phosphorylase (PNPase): Insights into the role of this RNase in pathogenicity
Biochimie. 2023 Oct 6:S0300-9084(23)00268-7. doi: 10.1016/j.biochi.2023.10.006. Online ahead of print.ABSTRACTRibonucleases are in charge of the processing, degradation and quality control of all cellular transcripts, which makes them crucial factors in RNA regulation. This post-transcriptional regulation allows bacteria to promptly react to different stress conditions and growth phase transitions, and also to produce the required virulence factors in pathogenic bacteria. Campylobacter jejuni is the main responsible for human gastroenteritis in the world. In this foodborne pathogen, exoribonuclease PNPase (CjPNP) is essent...
Source: Biochimie - October 8, 2023 Category: Biochemistry Authors: C átia Bárria Diogo Athayde Guillem Hernandez Leonor Fonseca Jorge Casinhas Tiago N Cordeiro Margarida Archer Cec ília M Arraiano Jos é A Brito Rute G Matos Source Type: research
A U2 snRNP-specific protein, U2A', is involved in stress response and drug resistance in Cryptococcus deneoformans
In this study, we identified a U2A' homologous protein as a component of the cryptococcal U2 snRNP, which was encoded by the LEA1 gene. Using the "suicide" CRISPR-Cas9 tool, we deleted the LEA1 gene in C. deneoformans JEC21 strain and obtained the disruption mutant lea1Δ. The mutant showed a hypersensitivity to 0.03 % sodium dodecyl sulfate, as well as disordered chitin distribution in cell wall observed with Calcofluor White staining, which collectively illustrated the function of U2A' in maintenance of cell wall integrity. Further examination showed that lea1Δ displayed a decreased tolerance to lower or elevated temper...
Source: Biochimie - October 8, 2023 Category: Biochemistry Authors: Lan Ma Xueqing Zhang Chenxi Li Xiaoyu Ma Xuan Zhao Xueru Zhao Ping Zhang Xudong Zhu Source Type: research
Coordination between human DNA polymerase β and apurinic/apyrimidinic endonuclease 1 in the course of DNA repair
Biochimie. 2023 Oct 6:S0300-9084(23)00270-5. doi: 10.1016/j.biochi.2023.10.007. Online ahead of print.ABSTRACTCoordination of enzymatic activities in the course of base excision repair (BER) is essential to ensure complete repair of damaged bases. Two major mechanisms underlying the coordination of BER are known today: the "passing the baton" model and a model of preassembled stable multiprotein repair complexes called "repairosomes." In this work, we aimed to elucidate the coordination between human apurinic/apyrimidinic (AP) endonuclease APE1 and DNA polymerase Polβ in BER through studying an impact of APE1 on Polβ-cat...
Source: Biochimie - October 8, 2023 Category: Biochemistry Authors: Artemiy S Bakman Stanislav S Boichenko Aleksandra A Kuznetsova Alexander A Ishchenko Murat Saparbaev Nikita A Kuznetsov Source Type: research
Lysophospholipase D from Thermocrispum limits psoriatic inflammation by hydrolyzing epidermal lysoplasmalogen produced by group IIF secreted phospholipase A < sub > 2 < /sub >
Biochimie. 2023 Oct 4:S0300-9084(23)00254-7. doi: 10.1016/j.biochi.2023.09.027. Online ahead of print.ABSTRACTEpidermal lipids play important roles in skin homeostasis and diseases. Psoriasis is an inflammatory disease characterized by keratinocyte hyperproliferation and Th17 immune responses. We previously reported that ethanolamine-type lysoplasmalogen (P-LPE), preferentially produced by group IIF secreted PLA2 (sPLA2-IIF/PLA2G2F) that is expressed in the suprabasal epidermis, promotes epidermal hyperplasia in psoriatic inflammation. Herein, we show that forcible degradation of epidermal P-LPE by topical application of r...
Source: Biochimie - October 6, 2023 Category: Biochemistry Authors: Haruka Hakoi Yoshimi Miki Saki Nomura Kimiko Nakajima Chiaki Terashima-Murase Takuya Takeichi Shigetoshi Sano Masashi Akiyama Shin-Ichi Sakasegawa Makoto Murakami Kei Yamamoto Source Type: research
Milk fat globule size: Unraveling the intricate relationship between metabolism, homeostasis, and stress signaling
Biochimie. 2023 Oct 4:S0300-9084(23)00257-2. doi: 10.1016/j.biochi.2023.10.003. Online ahead of print.ABSTRACTFat is an important component of milk which delivers energy, nutrients, and bioactive molecules from the lactating mother to the suckling neonate. Milk fat consists of a complex mixture of different types of lipids; hundreds of fatty acids, triglycerides, phospholipids, sphingolipids, cholesterol and cholesteryl ester, and glycoconjugates, secreted by the mammary gland epithelial cells (MEC) in the form of a lipid-protein assembly termed the milk fat globule (MFG). The mammary gland in general, and specifically tha...
Source: Biochimie - October 6, 2023 Category: Biochemistry Authors: Noam Tzirkel-Hancock Lior Sharabi Nurit Argov-Argaman Source Type: research
Lysophospholipase D from Thermocrispum limits psoriatic inflammation by hydrolyzing epidermal lysoplasmalogen produced by group IIF secreted phospholipase A < sub > 2 < /sub >
Biochimie. 2023 Oct 4:S0300-9084(23)00254-7. doi: 10.1016/j.biochi.2023.09.027. Online ahead of print.ABSTRACTEpidermal lipids play important roles in skin homeostasis and diseases. Psoriasis is an inflammatory disease characterized by keratinocyte hyperproliferation and Th17 immune responses. We previously reported that ethanolamine-type lysoplasmalogen (P-LPE), preferentially produced by group IIF secreted PLA2 (sPLA2-IIF/PLA2G2F) that is expressed in the suprabasal epidermis, promotes epidermal hyperplasia in psoriatic inflammation. Herein, we show that forcible degradation of epidermal P-LPE by topical application of r...
Source: Biochimie - October 6, 2023 Category: Biochemistry Authors: Haruka Hakoi Yoshimi Miki Saki Nomura Kimiko Nakajima Chiaki Terashima-Murase Takuya Takeichi Shigetoshi Sano Masashi Akiyama Shin-Ichi Sakasegawa Makoto Murakami Kei Yamamoto Source Type: research
Milk fat globule size: Unraveling the intricate relationship between metabolism, homeostasis, and stress signaling
Biochimie. 2023 Oct 4:S0300-9084(23)00257-2. doi: 10.1016/j.biochi.2023.10.003. Online ahead of print.ABSTRACTFat is an important component of milk which delivers energy, nutrients, and bioactive molecules from the lactating mother to the suckling neonate. Milk fat consists of a complex mixture of different types of lipids; hundreds of fatty acids, triglycerides, phospholipids, sphingolipids, cholesterol and cholesteryl ester, and glycoconjugates, secreted by the mammary gland epithelial cells (MEC) in the form of a lipid-protein assembly termed the milk fat globule (MFG). The mammary gland in general, and specifically tha...
Source: Biochimie - October 6, 2023 Category: Biochemistry Authors: Noam Tzirkel-Hancock Lior Sharabi Nurit Argov-Argaman Source Type: research
Characterization of novel truncated apolipoprotein A-I in human high-density lipoprotein generated by sequential treatment with myeloperoxidase and chymase
Biochimie. 2023 Sep 28:S0300-9084(23)00251-1. doi: 10.1016/j.biochi.2023.09.024. Online ahead of print.ABSTRACTHigh-density lipoprotein (HDL) cholesterol is a well-known biomarker, which has been associated with reduction in the risk of cardiovascular diseases (CVD). However, some HDL anti-atherosclerotic functions may be impaired without altered HDL-cholesterol (HDL-C) level via its dysfunctional proteins or other physiological reactions in vivo. We previously showed that activated mast cell-derived chymase could modestly cleave apolipoprotein A-I (apoA-I) in HDL3, and further easily cleave lipid-free apoA-I. In contrast,...
Source: Biochimie - September 29, 2023 Category: Biochemistry Authors: Shao-Jui Lai Takahiro Kameda Maasa Morita Yuka Yamagata Kaoruko Nishizaka Yuna Horiuchi Yukihiro Kobayashi Yoko Usami Jun-Jen Liu Takeshi Kasama Minoru Tozuka Ryunosuke Ohkawa Source Type: research
