The emerging functions of mini zinc finger (MIF) microproteins in seed plants: A minireview
Biochimie. 2023 Sep 16:S0300-9084(23)00243-2. doi: 10.1016/j.biochi.2023.09.016. Online ahead of print.ABSTRACTMini zinc fingers constitute a class of microproteins that appeared early in evolution and expanded in seeds plants. In this review, the phylogenetic history, the functions and the mode of action of MIFs in plants are reported and discussed. It appears that mini zinc finger microproteins play an important role in the control of plant development. They are involved in the control of cell division and expansion and in the switch between the determinate/indeterminate state of the meristems. They are involved in the r...
Source: Biochimie - September 18, 2023 Category: Biochemistry Authors: Marie Rose Ndella Thiaw Pascal Gantet Source Type: research
The emerging functions of mini zinc finger (MIF) microproteins in seed plants: A minireview
Biochimie. 2023 Sep 16:S0300-9084(23)00243-2. doi: 10.1016/j.biochi.2023.09.016. Online ahead of print.ABSTRACTMini zinc fingers constitute a class of microproteins that appeared early in evolution and expanded in seeds plants. In this review, the phylogenetic history, the functions and the mode of action of MIFs in plants are reported and discussed. It appears that mini zinc finger microproteins play an important role in the control of plant development. They are involved in the control of cell division and expansion and in the switch between the determinate/indeterminate state of the meristems. They are involved in the r...
Source: Biochimie - September 18, 2023 Category: Biochemistry Authors: Marie Rose Ndella Thiaw Pascal Gantet Source Type: research
The emerging functions of mini zinc finger (MIF) microproteins in seed plants: A minireview
Biochimie. 2023 Sep 16:S0300-9084(23)00243-2. doi: 10.1016/j.biochi.2023.09.016. Online ahead of print.ABSTRACTMini zinc fingers constitute a class of microproteins that appeared early in evolution and expanded in seeds plants. In this review, the phylogenetic history, the functions and the mode of action of MIFs in plants are reported and discussed. It appears that mini zinc finger microproteins play an important role in the control of plant development. They are involved in the control of cell division and expansion and in the switch between the determinate/indeterminate state of the meristems. They are involved in the r...
Source: Biochimie - September 18, 2023 Category: Biochemistry Authors: Marie Rose Ndella Thiaw Pascal Gantet Source Type: research
Using osteogenic medium in the in vitro evaluation of bone biomaterials: Artefacts due to a synergistic effect
This study analysed the proteomic profiles of human osteoblasts (HOb) cultured in the media with and without osteogenic agents (ascorbic acid and β-glycerol phosphate). The cells were incubated for 1 and 7 days, on their own or in contact with Ti. The comparative Perseus analysis identified 2544 proteins whose expression was affected by osteogenic agents. We observed that the OM strongly alters protein expression profiles with a complex impact on multiple pathways associated with adhesion, immunity, oxidative stress, coagulation, angiogenesis and osteogenesis. OM-triggered changes in the HOb intracellular energy productio...
Source: Biochimie - September 16, 2023 Category: Biochemistry Authors: Francisco Romero-Gavil án I ñaki García-Arnáez Andreia Cerqueira Carlos Arias-Mainer Mikel Azkargorta F élix Elortza Ra úl Izquierdo Maril ó Gurruchaga Isabel Go ñi Julio Suay Source Type: research
Human muscle cells sensitivity to chikungunya virus infection relies on their glycolysis activity and differentiation stage
Biochimie. 2023 Sep 14:S0300-9084(23)00214-6. doi: 10.1016/j.biochi.2023.09.005. Online ahead of print.ABSTRACTChanges to our environment have led to the emergence of human pathogens such as chikungunya virus. Chikungunya virus infection is today a major public health concern. It is a debilitating chronic disease impeding patients' mobility, affecting millions of people. Disease development relies on skeletal muscle infection. The importance of skeletal muscle in chikungunya virus infection led to the hypothesis that it could serve as a viral reservoir and could participate to virus persistence. Here we questioned the inte...
Source: Biochimie - September 16, 2023 Category: Biochemistry Authors: M Jaquet M Bengue K Lambert G Carnac D Miss é C Bisbal Source Type: research
Functional involvement of a conserved motif in the middle region of the human ribosomal protein eL42 in translation
Biochimie. 2023 Sep 14:S0300-9084(23)00219-5. doi: 10.1016/j.biochi.2023.09.010. Online ahead of print.ABSTRACTRibosomal protein eL42 (formerly known as L36A), a small protein of the large (60S) subunit of the eukaryotic ribosome, is a component of its exit (E) site. The residue K53 of this protein resides within the motif QSGYGGQTK mainly conserved in eukaryotes, and it is located in the immediate vicinity of the CCA-terminus of the ribosome-bound tRNA in the hybrid P/E state. To examine the role of this eL42 motif in translation, we obtained HEK293T cells producing the wild-type FLAG-tagged protein or its mutant forms wi...
Source: Biochimie - September 16, 2023 Category: Biochemistry Authors: Konstantin N Bulygin Alexey A Malygin Dmitri M Graifer Source Type: research
Using osteogenic medium in the in vitro evaluation of bone biomaterials: Artefacts due to a synergistic effect
This study analysed the proteomic profiles of human osteoblasts (HOb) cultured in the media with and without osteogenic agents (ascorbic acid and β-glycerol phosphate). The cells were incubated for 1 and 7 days, on their own or in contact with Ti. The comparative Perseus analysis identified 2544 proteins whose expression was affected by osteogenic agents. We observed that the OM strongly alters protein expression profiles with a complex impact on multiple pathways associated with adhesion, immunity, oxidative stress, coagulation, angiogenesis and osteogenesis. OM-triggered changes in the HOb intracellular energy productio...
Source: Biochimie - September 16, 2023 Category: Biochemistry Authors: Francisco Romero-Gavil án I ñaki García-Arnáez Andreia Cerqueira Carlos Arias-Mainer Mikel Azkargorta F élix Elortza Ra úl Izquierdo Maril ó Gurruchaga Isabel Go ñi Julio Suay Source Type: research
Human muscle cells sensitivity to chikungunya virus infection relies on their glycolysis activity and differentiation stage
Biochimie. 2023 Sep 14:S0300-9084(23)00214-6. doi: 10.1016/j.biochi.2023.09.005. Online ahead of print.ABSTRACTChanges to our environment have led to the emergence of human pathogens such as chikungunya virus. Chikungunya virus infection is today a major public health concern. It is a debilitating chronic disease impeding patients' mobility, affecting millions of people. Disease development relies on skeletal muscle infection. The importance of skeletal muscle in chikungunya virus infection led to the hypothesis that it could serve as a viral reservoir and could participate to virus persistence. Here we questioned the inte...
Source: Biochimie - September 16, 2023 Category: Biochemistry Authors: M Jaquet M Bengue K Lambert G Carnac D Miss é C Bisbal Source Type: research
Functional involvement of a conserved motif in the middle region of the human ribosomal protein eL42 in translation
Biochimie. 2023 Sep 14:S0300-9084(23)00219-5. doi: 10.1016/j.biochi.2023.09.010. Online ahead of print.ABSTRACTRibosomal protein eL42 (formerly known as L36A), a small protein of the large (60S) subunit of the eukaryotic ribosome, is a component of its exit (E) site. The residue K53 of this protein resides within the motif QSGYGGQTK mainly conserved in eukaryotes, and it is located in the immediate vicinity of the CCA-terminus of the ribosome-bound tRNA in the hybrid P/E state. To examine the role of this eL42 motif in translation, we obtained HEK293T cells producing the wild-type FLAG-tagged protein or its mutant forms wi...
Source: Biochimie - September 16, 2023 Category: Biochemistry Authors: Konstantin N Bulygin Alexey A Malygin Dmitri M Graifer Source Type: research
Structure of glyceraldehyde-3-phosphate dehydrogenase from Paracoccidioides lutzii in complex with an aldonic sugar acid
Biochimie. 2023 Sep 12:S0300-9084(23)00240-7. doi: 10.1016/j.biochi.2023.09.013. Online ahead of print.ABSTRACTThe pathogen Paracoccidioides lutzii (Pb01) is found in South America countries Colombia, Ecuador, Venezuela and Brazil, especially in the central, west, and north regions of the latter. It belongs to the Ajellomycetaceae family, Onygenales order, and is typically thermodimorphic, presenting yeast cells when it grows in animal tissues, but mycelia when in the environment, where it produces the infectious propagule. Moreover, this fungus is one of the etiologic agents of Paracoccidioidomycosis (PCM), the most impor...
Source: Biochimie - September 14, 2023 Category: Biochemistry Authors: Jonathan Heiler Hern ández-Prieto Viviane Paula Martini Jorge Iulek Source Type: research
Structure of glyceraldehyde-3-phosphate dehydrogenase from Paracoccidioides lutzii in complex with an aldonic sugar acid
Biochimie. 2023 Sep 12:S0300-9084(23)00240-7. doi: 10.1016/j.biochi.2023.09.013. Online ahead of print.ABSTRACTThe pathogen Paracoccidioides lutzii (Pb01) is found in South America countries Colombia, Ecuador, Venezuela and Brazil, especially in the central, west, and north regions of the latter. It belongs to the Ajellomycetaceae family, Onygenales order, and is typically thermodimorphic, presenting yeast cells when it grows in animal tissues, but mycelia when in the environment, where it produces the infectious propagule. Moreover, this fungus is one of the etiologic agents of Paracoccidioidomycosis (PCM), the most impor...
Source: Biochimie - September 14, 2023 Category: Biochemistry Authors: Jonathan Heiler Hern ández-Prieto Viviane Paula Martini Jorge Iulek Source Type: research
Structure of glyceraldehyde-3-phosphate dehydrogenase from Paracoccidioides lutzii in complex with an aldonic sugar acid
Biochimie. 2023 Sep 12:S0300-9084(23)00240-7. doi: 10.1016/j.biochi.2023.09.013. Online ahead of print.ABSTRACTThe pathogen Paracoccidioides lutzii (Pb01) is found in South America countries Colombia, Ecuador, Venezuela and Brazil, especially in the central, west, and north regions of the latter. It belongs to the Ajellomycetaceae family, Onygenales order, and is typically thermodimorphic, presenting yeast cells when it grows in animal tissues, but mycelia when in the environment, where it produces the infectious propagule. Moreover, this fungus is one of the etiologic agents of Paracoccidioidomycosis (PCM), the most impor...
Source: Biochimie - September 14, 2023 Category: Biochemistry Authors: Jonathan Heiler Hern ández-Prieto Viviane Paula Martini Jorge Iulek Source Type: research
Antiplatelet mechanism of a subtilisin-like serine protease from Solanum tuberosum (StSBTc-3)
Biochimie. 2023 Sep 11:S0300-9084(23)00238-9. doi: 10.1016/j.biochi.2023.09.011. Online ahead of print.ABSTRACTThe aims of this study are to characterize the antiplatelet activity of StSBTc-3, a potato serine protease with fibrino(geno) lytic activity, and to provide information on its mechanism of action. The results obtained show that StSBTc-3 inhibits clot retraction and prevents platelet aggregation induced by thrombin, convulxin, and A23187. Platelet aggregation inhibition occurs in a dose-dependent manner and is not affected by inactivation of StSBTc-3 with the inhibitor of serine proteases phenylmethylsulfonyl fluor...
Source: Biochimie - September 13, 2023 Category: Biochemistry Authors: Alfonso Pepe Florencia Rocio Tito Maria Gabriela Guevara Source Type: research
Isolation of the AccCDK8 gene of Apis cerana cerana and its functional analysis under pesticide and heavy metal stress
Biochimie. 2023 Sep 11:S0300-9084(23)00239-0. doi: 10.1016/j.biochi.2023.09.012. Online ahead of print.ABSTRACTEnvironmental pollution has gained negative attention in recent years. The pesticides and heavy metals are top list of environmental toxicants directly endangering the survival and development of Apis cerana cerana. Cyclin-dependent kinases (CDKs) are heteromeric serine/threonine kinases that participate in cell cycle regulation and have a vital role in pesticide and heavy metal stress in Apis cerana cerana. In this experiment, we filtered out CDK8 gene from Apis cerana cerana (AccCDK8) and investigated its functi...
Source: Biochimie - September 13, 2023 Category: Biochemistry Authors: Jing Li Dezheng Guo Jinhao Bai Hongfang Wang Chen Wang Ying Wang Xingqi Guo Baohua Xu Zhenguo Liu Source Type: research
Dissolved metal ions and mineral-liposome hybrid systems: Underlying interactions, synthesis, and characterization
Biochimie. 2023 Sep 10:S0300-9084(23)00218-3. doi: 10.1016/j.biochi.2023.09.009. Online ahead of print.ABSTRACTLiposomes are versatile lipid-based vesicles with interesting physicochemical properties, making them excellent candidates for interdisciplinary applications in the medicinal, biological, and environmental sciences. The synthesis of mineral-liposome hybrid systems lends normally inert vesicles with the catalytic, magnetic, electrical, and optical properties of the integrated mineral species. Such applications require an understanding of the physicochemical interactions between organic molecules and inorganic cryst...
Source: Biochimie - September 12, 2023 Category: Biochemistry Authors: Nil Gaudu Orion Farr Georges Ona-Nguema Simon Duval Source Type: research