In-depth immunorecognition and neutralization analyses of Micrurus mipartitus and M. dumerilii venoms and toxins by a commercial antivenom
Biochimie. 2023 Oct 14:S0300-9084(23)00272-9. doi: 10.1016/j.biochi.2023.10.009. Online ahead of print.ABSTRACTIn Colombia, the Micrurus genus comprises 30 species, including M. mipartitus and M. dumerilii, which are of major clinical relevance due to their wide geographical distribution and the number of snakebites inflicted by them. These neurotoxic envenomations are characterized by neuromuscular paralysis attributed to venom components such as three-finger toxins (3FTx) and phospholipases (PLA2). Additionally, there is limited information available on the neutralizing coverage of commercially available antivenoms, unde...
Source: Biochimie - October 16, 2023 Category: Biochemistry Authors: Juan Diego Piedrahita Ana Cardona-Ruda Jaime Andr és Pereañez Paola Rey-Su árez Source Type: research
High-salt diet induces dyslipidemia through the SREBP2/PCSK9 pathway in dahl salt-sensitive rats
This study, for the first time, shows that an HSD induces dyslipidemia through activation of the SREBP2/PCSK9 pathway, providing new insights into the prevention and treatment of dyslipidemia in patients with salt sensitivity.PMID:37844755 | DOI:10.1016/j.biochi.2023.10.001 (Source: Biochimie)
Source: Biochimie - October 16, 2023 Category: Biochemistry Authors: Ya-Nan Ou-Yang Fen-Fen Deng Yun-Jia Wang Meng Chen Peng-Fei Yang Zhe Yang Zhongmin Tian Source Type: research
In-depth immunorecognition and neutralization analyses of Micrurus mipartitus and M. dumerilii venoms and toxins by a commercial antivenom
Biochimie. 2023 Oct 14:S0300-9084(23)00272-9. doi: 10.1016/j.biochi.2023.10.009. Online ahead of print.ABSTRACTIn Colombia, the Micrurus genus comprises 30 species, including M. mipartitus and M. dumerilii, which are of major clinical relevance due to their wide geographical distribution and the number of snakebites inflicted by them. These neurotoxic envenomations are characterized by neuromuscular paralysis attributed to venom components such as three-finger toxins (3FTx) and phospholipases (PLA2). Additionally, there is limited information available on the neutralizing coverage of commercially available antivenoms, unde...
Source: Biochimie - October 16, 2023 Category: Biochemistry Authors: Juan Diego Piedrahita Ana Cardona-Ruda Jaime Andr és Pereañez Paola Rey-Su árez Source Type: research
High-salt diet induces dyslipidemia through the SREBP2/PCSK9 pathway in dahl salt-sensitive rats
This study, for the first time, shows that an HSD induces dyslipidemia through activation of the SREBP2/PCSK9 pathway, providing new insights into the prevention and treatment of dyslipidemia in patients with salt sensitivity.PMID:37844755 | DOI:10.1016/j.biochi.2023.10.001 (Source: Biochimie)
Source: Biochimie - October 16, 2023 Category: Biochemistry Authors: Ya-Nan Ou-Yang Fen-Fen Deng Yun-Jia Wang Meng Chen Peng-Fei Yang Zhe Yang Zhongmin Tian Source Type: research
In-depth immunorecognition and neutralization analyses of Micrurus mipartitus and M. dumerilii venoms and toxins by a commercial antivenom
Biochimie. 2023 Oct 14:S0300-9084(23)00272-9. doi: 10.1016/j.biochi.2023.10.009. Online ahead of print.ABSTRACTIn Colombia, the Micrurus genus comprises 30 species, including M. mipartitus and M. dumerilii, which are of major clinical relevance due to their wide geographical distribution and the number of snakebites inflicted by them. These neurotoxic envenomations are characterized by neuromuscular paralysis attributed to venom components such as three-finger toxins (3FTx) and phospholipases (PLA2). Additionally, there is limited information available on the neutralizing coverage of commercially available antivenoms, unde...
Source: Biochimie - October 16, 2023 Category: Biochemistry Authors: Juan Diego Piedrahita Ana Cardona-Ruda Jaime Andr és Pereañez Paola Rey-Su árez Source Type: research
High-salt diet induces dyslipidemia through the SREBP2/PCSK9 pathway in dahl salt-sensitive rats
This study, for the first time, shows that an HSD induces dyslipidemia through activation of the SREBP2/PCSK9 pathway, providing new insights into the prevention and treatment of dyslipidemia in patients with salt sensitivity.PMID:37844755 | DOI:10.1016/j.biochi.2023.10.001 (Source: Biochimie)
Source: Biochimie - October 16, 2023 Category: Biochemistry Authors: Ya-Nan Ou-Yang Fen-Fen Deng Yun-Jia Wang Meng Chen Peng-Fei Yang Zhe Yang Zhongmin Tian Source Type: research
A novel metalloproteinase-derived cryptide from Bothrops cotiara venom inhibits angiotensin-converting enzyme activity
In this study, we used mass spectrometry-based peptidomics to sequence 197 peptides in the venom of Bothrops cotiara, including a novel 7-residue peptide derived from a snake venom metalloproteinase. This peptide, named Bc-7a, features a pyroglutamic acid at the N-terminal and a PFR motif at the C-terminal, homologous to bradykinin. Using FRET (fluorescence resonance energy transfer) substrate assays, we demonstrated that Bc-7a strongly inhibits the two domains of angiotensin converting enzyme (Ki < 1 μM). Our findings contribute to the repertoire of biologically active peptides from snake venoms capable of inhibiting ...
Source: Biochimie - October 15, 2023 Category: Biochemistry Authors: Jackson Gabriel Miyamoto Eduardo Shigueo Kitano Andr é Zelanis Pedro Gabriel Nachtigall In ácio Junqueira-de-Azevedo S ávio Stefanini Sant'Anna Rog ério Lauria da Silva Patr ícia Alessandra Bersanetti Adriana Karaoglanovic Carmona Pedro Jos é Barbos Source Type: research
A novel metalloproteinase-derived cryptide from Bothrops cotiara venom inhibits angiotensin-converting enzyme activity
In this study, we used mass spectrometry-based peptidomics to sequence 197 peptides in the venom of Bothrops cotiara, including a novel 7-residue peptide derived from a snake venom metalloproteinase. This peptide, named Bc-7a, features a pyroglutamic acid at the N-terminal and a PFR motif at the C-terminal, homologous to bradykinin. Using FRET (fluorescence resonance energy transfer) substrate assays, we demonstrated that Bc-7a strongly inhibits the two domains of angiotensin converting enzyme (Ki < 1 μM). Our findings contribute to the repertoire of biologically active peptides from snake venoms capable of inhibiting ...
Source: Biochimie - October 15, 2023 Category: Biochemistry Authors: Jackson Gabriel Miyamoto Eduardo Shigueo Kitano Andr é Zelanis Pedro Gabriel Nachtigall In ácio Junqueira-de-Azevedo S ávio Stefanini Sant'Anna Rog ério Lauria da Silva Patr ícia Alessandra Bersanetti Adriana Karaoglanovic Carmona Pedro Jos é Barbos Source Type: research
Transactivation by partial function P53 family mutants is increased by the presence of G-quadruplexes at a promoter site
Biochimie. 2023 Oct 12:S0300-9084(23)00253-5. doi: 10.1016/j.biochi.2023.09.026. Online ahead of print.ABSTRACTThe effect of mutations in the P53 family of transcription factors on their biological functions, including partial or complete loss of transcriptional activity, has been confirmed several times. At present, P53 family proteins showing partial loss of activity appear to be promising potential candidates for the development of novel therapeutic strategies which could restore their transcriptional activity. In this context, it is important to employ tools to precisely monitor their activity; in relation to this, non...
Source: Biochimie - October 14, 2023 Category: Biochemistry Authors: Mat úš Vojsovič Libu še Kratochvilová Nat ália Valková Lucie Šislerová Zeinab El Rashed Paola Menichini Alberto Inga Paola Monti V áclav Brázda Source Type: research
Transactivation by partial function P53 family mutants is increased by the presence of G-quadruplexes at a promoter site
Biochimie. 2023 Oct 12:S0300-9084(23)00253-5. doi: 10.1016/j.biochi.2023.09.026. Online ahead of print.ABSTRACTThe effect of mutations in the P53 family of transcription factors on their biological functions, including partial or complete loss of transcriptional activity, has been confirmed several times. At present, P53 family proteins showing partial loss of activity appear to be promising potential candidates for the development of novel therapeutic strategies which could restore their transcriptional activity. In this context, it is important to employ tools to precisely monitor their activity; in relation to this, non...
Source: Biochimie - October 14, 2023 Category: Biochemistry Authors: Mat úš Vojsovič Libu še Kratochvilová Nat ália Valková Lucie Šislerová Zeinab El Rashed Paola Menichini Alberto Inga Paola Monti V áclav Brázda Source Type: research
Desmin and its molecular chaperone, the αB-crystallin: How post-translational modifications modulate their functions in heart and skeletal muscles?
Biochimie. 2023 Oct 10:S0300-9084(23)00256-0. doi: 10.1016/j.biochi.2023.10.002. Online ahead of print.ABSTRACTMaintenance of the highly organized striated muscle tissue requires a cell-wide dynamic network through protein-protein interactions providing an effective mechanochemical integrator of morphology and function. Through a continuous and complex trans-cytoplasmic network, desmin intermediate filaments ensure this essential role in heart and in skeletal muscle. Besides their role in the maintenance of cell shape and architecture (permitting contractile activity efficiency and conferring resistance towards mechanical ...
Source: Biochimie - October 12, 2023 Category: Biochemistry Authors: Charlotte Claeyssen Nathan Bulangalire Bruno Bastide Onnik Agbulut Caroline Cieniewski-Bernard Source Type: research
Desmin and its molecular chaperone, the αB-crystallin: How post-translational modifications modulate their functions in heart and skeletal muscles?
Biochimie. 2023 Oct 10:S0300-9084(23)00256-0. doi: 10.1016/j.biochi.2023.10.002. Online ahead of print.ABSTRACTMaintenance of the highly organized striated muscle tissue requires a cell-wide dynamic network through protein-protein interactions providing an effective mechanochemical integrator of morphology and function. Through a continuous and complex trans-cytoplasmic network, desmin intermediate filaments ensure this essential role in heart and in skeletal muscle. Besides their role in the maintenance of cell shape and architecture (permitting contractile activity efficiency and conferring resistance towards mechanical ...
Source: Biochimie - October 12, 2023 Category: Biochemistry Authors: Charlotte Claeyssen Nathan Bulangalire Bruno Bastide Onnik Agbulut Caroline Cieniewski-Bernard Source Type: research
Flexibility of active center affects thermostability and activity of Penicillium canescens xylanase E
Biochimie. 2023 Oct 9:S0300-9084(23)00258-4. doi: 10.1016/j.biochi.2023.10.004. Online ahead of print.ABSTRACTXylanases are used in several industrial applications, such as feed additives, the bleaching of pulp and paper, and the production of bread, food, and drinks. Xylanases are required to remain active after heat treatment at 80-90 °C for 30 s to several minutes due to the conditions of feed pelleting. Also, xylanases need to be active at 60-70 °C for several hours while bleaching of pulp and paper or manufacturing of bread, food, and drinks is performed. Xylanases of the glycoside hydrolase family GH10 are good can...
Source: Biochimie - October 11, 2023 Category: Biochemistry Authors: Anna Dotsenko Igor Sinelnikov Aleksandra Rozhkova Ivan Zorov Arkady Sinitsyn Source Type: research
Alpha hemolysin of E. coli induces hemolysis of human erythrocytes independently of toxin interaction with membrane proteins
Biochimie. 2023 Oct 9:S0300-9084(23)00271-7. doi: 10.1016/j.biochi.2023.10.008. Online ahead of print.ABSTRACTAlpha hemolysin (HlyA) is a hemolytic and cytotoxic protein secreted by uropathogenic strains of E. coli. The role of glycophorins (GPs) as putative receptors for HlyA binding to red blood cells (RBCs) has been debated. Experiments using anti-GPA/GPB antibodies and a GPA-specific epitope nanobody to block HlyA-GP binding on hRBCs, showed no effect on hemolytic activity. Similarly, the hemolysis induced by HlyA remained unaffected when hRBCs from a GPAnull/GPBnull variant were used. Surface Plasmon Resonance experim...
Source: Biochimie - October 11, 2023 Category: Biochemistry Authors: Luc ía Cané Nicol ás Andrés Saffioti Sandrine Genetet Mar ía Antonieta Daza Millone Mariano A Ostuni Pablo J Schwarzbaum Isabelle Mouro-Chanteloup Vanesa Herlax Source Type: research
Flexibility of active center affects thermostability and activity of Penicillium canescens xylanase E
Biochimie. 2023 Oct 9:S0300-9084(23)00258-4. doi: 10.1016/j.biochi.2023.10.004. Online ahead of print.ABSTRACTXylanases are used in several industrial applications, such as feed additives, the bleaching of pulp and paper, and the production of bread, food, and drinks. Xylanases are required to remain active after heat treatment at 80-90 °C for 30 s to several minutes due to the conditions of feed pelleting. Also, xylanases need to be active at 60-70 °C for several hours while bleaching of pulp and paper or manufacturing of bread, food, and drinks is performed. Xylanases of the glycoside hydrolase family GH10 are good can...
Source: Biochimie - October 11, 2023 Category: Biochemistry Authors: Anna Dotsenko Igor Sinelnikov Aleksandra Rozhkova Ivan Zorov Arkady Sinitsyn Source Type: research
