Sensing of DNA modifications by pAgo proteins in vitro
Biochimie. 2023 Dec 19;220:39-47. doi: 10.1016/j.biochi.2023.12.006. Online ahead of print.ABSTRACTMany prokaryotic Argonaute (pAgo) proteins act as programmable nucleases that use small guide DNAs for recognition and cleavage of complementary target DNA. Recent studies suggested that pAgos participate in cell defense against invader DNA and may also be involved in other genetic processes, including DNA replication and repair. The ability of pAgos to recognize specific targets potentially make them an invaluable tool for DNA manipulations. Here, we demonstrate that DNA-guided DNA-targeting pAgo nucleases from three bacteri...
Source: Biochimie - December 21, 2023 Category: Biochemistry Authors: Margarita Beskrovnaia Aleksei Agapov Kristina Makasheva Dmitry O Zharkov Daria Esyunina Andrey Kulbachinskiy Source Type: research
Effects of osmolytes under crowding conditions on the properties of muscle glycogen phosphorylase b
Biochimie. 2023 Dec 20;220:48-57. doi: 10.1016/j.biochi.2023.12.005. Online ahead of print.ABSTRACTThe study of the relationship between the activity and stability of enzymes under crowding conditions in the presence of osmolytes is important for understanding the functioning of a living cell. The effect of osmolytes (trehalose and betaine) on the secondary and tertiary structure and activity of muscle glycogen phosphorylase b (Phb) under crowding conditions created by PEG 2000 and PEG 20000 was investigated using dynamic light scattering, differential scanning calorimetry, circular dichroism spectroscopy, fluorimetry and ...
Source: Biochimie - December 21, 2023 Category: Biochemistry Authors: Valeriya V Mikhaylova Tatiana B Eronina Source Type: research
Sensing of DNA modifications by pAgo proteins in vitro
Biochimie. 2023 Dec 19;220:39-47. doi: 10.1016/j.biochi.2023.12.006. Online ahead of print.ABSTRACTMany prokaryotic Argonaute (pAgo) proteins act as programmable nucleases that use small guide DNAs for recognition and cleavage of complementary target DNA. Recent studies suggested that pAgos participate in cell defense against invader DNA and may also be involved in other genetic processes, including DNA replication and repair. The ability of pAgos to recognize specific targets potentially make them an invaluable tool for DNA manipulations. Here, we demonstrate that DNA-guided DNA-targeting pAgo nucleases from three bacteri...
Source: Biochimie - December 21, 2023 Category: Biochemistry Authors: Margarita Beskrovnaia Aleksei Agapov Kristina Makasheva Dmitry O Zharkov Daria Esyunina Andrey Kulbachinskiy Source Type: research
Effects of osmolytes under crowding conditions on the properties of muscle glycogen phosphorylase b
Biochimie. 2023 Dec 20;220:48-57. doi: 10.1016/j.biochi.2023.12.005. Online ahead of print.ABSTRACTThe study of the relationship between the activity and stability of enzymes under crowding conditions in the presence of osmolytes is important for understanding the functioning of a living cell. The effect of osmolytes (trehalose and betaine) on the secondary and tertiary structure and activity of muscle glycogen phosphorylase b (Phb) under crowding conditions created by PEG 2000 and PEG 20000 was investigated using dynamic light scattering, differential scanning calorimetry, circular dichroism spectroscopy, fluorimetry and ...
Source: Biochimie - December 21, 2023 Category: Biochemistry Authors: Valeriya V Mikhaylova Tatiana B Eronina Source Type: research
Sensing of DNA modifications by pAgo proteins in vitro
Biochimie. 2023 Dec 19;220:39-47. doi: 10.1016/j.biochi.2023.12.006. Online ahead of print.ABSTRACTMany prokaryotic Argonaute (pAgo) proteins act as programmable nucleases that use small guide DNAs for recognition and cleavage of complementary target DNA. Recent studies suggested that pAgos participate in cell defense against invader DNA and may also be involved in other genetic processes, including DNA replication and repair. The ability of pAgos to recognize specific targets potentially make them an invaluable tool for DNA manipulations. Here, we demonstrate that DNA-guided DNA-targeting pAgo nucleases from three bacteri...
Source: Biochimie - December 21, 2023 Category: Biochemistry Authors: Margarita Beskrovnaia Aleksei Agapov Kristina Makasheva Dmitry O Zharkov Daria Esyunina Andrey Kulbachinskiy Source Type: research
Effects of osmolytes under crowding conditions on the properties of muscle glycogen phosphorylase b
Biochimie. 2023 Dec 20;220:48-57. doi: 10.1016/j.biochi.2023.12.005. Online ahead of print.ABSTRACTThe study of the relationship between the activity and stability of enzymes under crowding conditions in the presence of osmolytes is important for understanding the functioning of a living cell. The effect of osmolytes (trehalose and betaine) on the secondary and tertiary structure and activity of muscle glycogen phosphorylase b (Phb) under crowding conditions created by PEG 2000 and PEG 20000 was investigated using dynamic light scattering, differential scanning calorimetry, circular dichroism spectroscopy, fluorimetry and ...
Source: Biochimie - December 21, 2023 Category: Biochemistry Authors: Valeriya V Mikhaylova Tatiana B Eronina Source Type: research
Sensing of DNA modifications by pAgo proteins in vitro
Biochimie. 2023 Dec 19;220:39-47. doi: 10.1016/j.biochi.2023.12.006. Online ahead of print.ABSTRACTMany prokaryotic Argonaute (pAgo) proteins act as programmable nucleases that use small guide DNAs for recognition and cleavage of complementary target DNA. Recent studies suggested that pAgos participate in cell defense against invader DNA and may also be involved in other genetic processes, including DNA replication and repair. The ability of pAgos to recognize specific targets potentially make them an invaluable tool for DNA manipulations. Here, we demonstrate that DNA-guided DNA-targeting pAgo nucleases from three bacteri...
Source: Biochimie - December 21, 2023 Category: Biochemistry Authors: Margarita Beskrovnaia Aleksei Agapov Kristina Makasheva Dmitry O Zharkov Daria Esyunina Andrey Kulbachinskiy Source Type: research
Effects of osmolytes under crowding conditions on the properties of muscle glycogen phosphorylase b
Biochimie. 2023 Dec 19:S0300-9084(23)00328-0. doi: 10.1016/j.biochi.2023.12.005. Online ahead of print.ABSTRACTThe study of the relationship between the activity and stability of enzymes under crowding conditions in the presence of osmolytes is important for understanding the functioning of a living cell. The effect of osmolytes (trehalose and betaine) on the secondary and tertiary structure and activity of muscle glycogen phosphorylase b (Phb) under crowding conditions created by PEG 2000 and PEG 20000 was investigated using dynamic light scattering, differential scanning calorimetry, circular dichroism spectroscopy, fluo...
Source: Biochimie - December 21, 2023 Category: Biochemistry Authors: Valeriya V Mikhaylova Tatiana B Eronina Source Type: research
Sensing of DNA modifications by pAgo proteins in vitro
Biochimie. 2023 Dec 19:S0300-9084(23)00330-9. doi: 10.1016/j.biochi.2023.12.006. Online ahead of print.ABSTRACTMany prokaryotic Argonaute (pAgo) proteins act as programmable nucleases that use small guide DNAs for recognition and cleavage of complementary target DNA. Recent studies suggested that pAgos participate in cell defense against invader DNA and may also be involved in other genetic processes, including DNA replication and repair. The ability of pAgos to recognize specific targets potentially make them an invaluable tool for DNA manipulations. Here, we demonstrate that DNA-guided DNA-targeting pAgo nucleases from t...
Source: Biochimie - December 21, 2023 Category: Biochemistry Authors: Margarita Beskrovnaia Aleksei Agapov Kristina Makasheva Dmitry O Zharkov Daria Esyunina Andrey Kulbachinskiy Source Type: research
Effects of osmolytes under crowding conditions on the properties of muscle glycogen phosphorylase b
Biochimie. 2023 Dec 19:S0300-9084(23)00328-0. doi: 10.1016/j.biochi.2023.12.005. Online ahead of print.ABSTRACTThe study of the relationship between the activity and stability of enzymes under crowding conditions in the presence of osmolytes is important for understanding the functioning of a living cell. The effect of osmolytes (trehalose and betaine) on the secondary and tertiary structure and activity of muscle glycogen phosphorylase b (Phb) under crowding conditions created by PEG 2000 and PEG 20000 was investigated using dynamic light scattering, differential scanning calorimetry, circular dichroism spectroscopy, fluo...
Source: Biochimie - December 21, 2023 Category: Biochemistry Authors: Valeriya V Mikhaylova Tatiana B Eronina Source Type: research
Sensing of DNA modifications by pAgo proteins in vitro
Biochimie. 2023 Dec 19:S0300-9084(23)00330-9. doi: 10.1016/j.biochi.2023.12.006. Online ahead of print.ABSTRACTMany prokaryotic Argonaute (pAgo) proteins act as programmable nucleases that use small guide DNAs for recognition and cleavage of complementary target DNA. Recent studies suggested that pAgos participate in cell defense against invader DNA and may also be involved in other genetic processes, including DNA replication and repair. The ability of pAgos to recognize specific targets potentially make them an invaluable tool for DNA manipulations. Here, we demonstrate that DNA-guided DNA-targeting pAgo nucleases from t...
Source: Biochimie - December 21, 2023 Category: Biochemistry Authors: Margarita Beskrovnaia Aleksei Agapov Kristina Makasheva Dmitry O Zharkov Daria Esyunina Andrey Kulbachinskiy Source Type: research
Effects of osmolytes under crowding conditions on the properties of muscle glycogen phosphorylase b
Biochimie. 2023 Dec 19:S0300-9084(23)00328-0. doi: 10.1016/j.biochi.2023.12.005. Online ahead of print.ABSTRACTThe study of the relationship between the activity and stability of enzymes under crowding conditions in the presence of osmolytes is important for understanding the functioning of a living cell. The effect of osmolytes (trehalose and betaine) on the secondary and tertiary structure and activity of muscle glycogen phosphorylase b (Phb) under crowding conditions created by PEG 2000 and PEG 20000 was investigated using dynamic light scattering, differential scanning calorimetry, circular dichroism spectroscopy, fluo...
Source: Biochimie - December 21, 2023 Category: Biochemistry Authors: Valeriya V Mikhaylova Tatiana B Eronina Source Type: research
Sensing of DNA modifications by pAgo proteins in vitro
Biochimie. 2023 Dec 19:S0300-9084(23)00330-9. doi: 10.1016/j.biochi.2023.12.006. Online ahead of print.ABSTRACTMany prokaryotic Argonaute (pAgo) proteins act as programmable nucleases that use small guide DNAs for recognition and cleavage of complementary target DNA. Recent studies suggested that pAgos participate in cell defense against invader DNA and may also be involved in other genetic processes, including DNA replication and repair. The ability of pAgos to recognize specific targets potentially make them an invaluable tool for DNA manipulations. Here, we demonstrate that DNA-guided DNA-targeting pAgo nucleases from t...
Source: Biochimie - December 21, 2023 Category: Biochemistry Authors: Margarita Beskrovnaia Aleksei Agapov Kristina Makasheva Dmitry O Zharkov Daria Esyunina Andrey Kulbachinskiy Source Type: research
Effects of osmolytes under crowding conditions on the properties of muscle glycogen phosphorylase b
Biochimie. 2023 Dec 19:S0300-9084(23)00328-0. doi: 10.1016/j.biochi.2023.12.005. Online ahead of print.ABSTRACTThe study of the relationship between the activity and stability of enzymes under crowding conditions in the presence of osmolytes is important for understanding the functioning of a living cell. The effect of osmolytes (trehalose and betaine) on the secondary and tertiary structure and activity of muscle glycogen phosphorylase b (Phb) under crowding conditions created by PEG 2000 and PEG 20000 was investigated using dynamic light scattering, differential scanning calorimetry, circular dichroism spectroscopy, fluo...
Source: Biochimie - December 21, 2023 Category: Biochemistry Authors: Valeriya V Mikhaylova Tatiana B Eronina Source Type: research
Sensing of DNA modifications by pAgo proteins in vitro
Biochimie. 2023 Dec 19:S0300-9084(23)00330-9. doi: 10.1016/j.biochi.2023.12.006. Online ahead of print.ABSTRACTMany prokaryotic Argonaute (pAgo) proteins act as programmable nucleases that use small guide DNAs for recognition and cleavage of complementary target DNA. Recent studies suggested that pAgos participate in cell defense against invader DNA and may also be involved in other genetic processes, including DNA replication and repair. The ability of pAgos to recognize specific targets potentially make them an invaluable tool for DNA manipulations. Here, we demonstrate that DNA-guided DNA-targeting pAgo nucleases from t...
Source: Biochimie - December 21, 2023 Category: Biochemistry Authors: Margarita Beskrovnaia Aleksei Agapov Kristina Makasheva Dmitry O Zharkov Daria Esyunina Andrey Kulbachinskiy Source Type: research
