Sensing of DNA modifications by pAgo proteins in vitro
Biochimie. 2023 Dec 19:S0300-9084(23)00330-9. doi: 10.1016/j.biochi.2023.12.006. Online ahead of print.ABSTRACTMany prokaryotic Argonaute (pAgo) proteins act as programmable nucleases that use small guide DNAs for recognition and cleavage of complementary target DNA. Recent studies suggested that pAgos participate in cell defense against invader DNA and may also be involved in other genetic processes, including DNA replication and repair. The ability of pAgos to recognize specific targets potentially make them an invaluable tool for DNA manipulations. Here, we demonstrate that DNA-guided DNA-targeting pAgo nucleases from t...
Source: Biochimie - December 21, 2023 Category: Biochemistry Authors: Margarita Beskrovnaia Aleksei Agapov Kristina Makasheva Dmitry O Zharkov Daria Esyunina Andrey Kulbachinskiy Source Type: research

Effects of osmolytes under crowding conditions on the properties of muscle glycogen phosphorylase b
Biochimie. 2023 Dec 19:S0300-9084(23)00328-0. doi: 10.1016/j.biochi.2023.12.005. Online ahead of print.ABSTRACTThe study of the relationship between the activity and stability of enzymes under crowding conditions in the presence of osmolytes is important for understanding the functioning of a living cell. The effect of osmolytes (trehalose and betaine) on the secondary and tertiary structure and activity of muscle glycogen phosphorylase b (Phb) under crowding conditions created by PEG 2000 and PEG 20000 was investigated using dynamic light scattering, differential scanning calorimetry, circular dichroism spectroscopy, fluo...
Source: Biochimie - December 21, 2023 Category: Biochemistry Authors: Valeriya V Mikhaylova Tatiana B Eronina Source Type: research

Sensing of DNA modifications by pAgo proteins in vitro
Biochimie. 2023 Dec 19:S0300-9084(23)00330-9. doi: 10.1016/j.biochi.2023.12.006. Online ahead of print.ABSTRACTMany prokaryotic Argonaute (pAgo) proteins act as programmable nucleases that use small guide DNAs for recognition and cleavage of complementary target DNA. Recent studies suggested that pAgos participate in cell defense against invader DNA and may also be involved in other genetic processes, including DNA replication and repair. The ability of pAgos to recognize specific targets potentially make them an invaluable tool for DNA manipulations. Here, we demonstrate that DNA-guided DNA-targeting pAgo nucleases from t...
Source: Biochimie - December 21, 2023 Category: Biochemistry Authors: Margarita Beskrovnaia Aleksei Agapov Kristina Makasheva Dmitry O Zharkov Daria Esyunina Andrey Kulbachinskiy Source Type: research

Effects of osmolytes under crowding conditions on the properties of muscle glycogen phosphorylase b
Biochimie. 2023 Dec 19:S0300-9084(23)00328-0. doi: 10.1016/j.biochi.2023.12.005. Online ahead of print.ABSTRACTThe study of the relationship between the activity and stability of enzymes under crowding conditions in the presence of osmolytes is important for understanding the functioning of a living cell. The effect of osmolytes (trehalose and betaine) on the secondary and tertiary structure and activity of muscle glycogen phosphorylase b (Phb) under crowding conditions created by PEG 2000 and PEG 20000 was investigated using dynamic light scattering, differential scanning calorimetry, circular dichroism spectroscopy, fluo...
Source: Biochimie - December 21, 2023 Category: Biochemistry Authors: Valeriya V Mikhaylova Tatiana B Eronina Source Type: research

Sensing of DNA modifications by pAgo proteins in vitro
Biochimie. 2023 Dec 19:S0300-9084(23)00330-9. doi: 10.1016/j.biochi.2023.12.006. Online ahead of print.ABSTRACTMany prokaryotic Argonaute (pAgo) proteins act as programmable nucleases that use small guide DNAs for recognition and cleavage of complementary target DNA. Recent studies suggested that pAgos participate in cell defense against invader DNA and may also be involved in other genetic processes, including DNA replication and repair. The ability of pAgos to recognize specific targets potentially make them an invaluable tool for DNA manipulations. Here, we demonstrate that DNA-guided DNA-targeting pAgo nucleases from t...
Source: Biochimie - December 21, 2023 Category: Biochemistry Authors: Margarita Beskrovnaia Aleksei Agapov Kristina Makasheva Dmitry O Zharkov Daria Esyunina Andrey Kulbachinskiy Source Type: research

Leishmania donovani mevalonate kinase regulates host actin for inducing phagocytosis
Biochimie. 2023 Dec 18:S0300-9084(23)00326-7. doi: 10.1016/j.biochi.2023.12.003. Online ahead of print.ABSTRACTDespite the well-established role of macrophages in phagocytosing Leishmania, the contribution of the parasite to this process is not well understood. Present study provides insights into the mechanism underlying the MVK-induced entry of L. donovani and improve our knowledge of host-pathogen interactions. We have discussed Mevalonate kinase (MVK)-induced actin reorganization, modulation of signalling pathways and host cell functions. Our results show that LdMVK gains access to macrophage cytosol and induces actin ...
Source: Biochimie - December 20, 2023 Category: Biochemistry Authors: Tanvir Bamra Taj Shafi Sushmita Das Manjay Kumar Pradeep Das Source Type: research

Leishmania donovani mevalonate kinase regulates host actin for inducing phagocytosis
Biochimie. 2023 Dec 18:S0300-9084(23)00326-7. doi: 10.1016/j.biochi.2023.12.003. Online ahead of print.ABSTRACTDespite the well-established role of macrophages in phagocytosing Leishmania, the contribution of the parasite to this process is not well understood. Present study provides insights into the mechanism underlying the MVK-induced entry of L. donovani and improve our knowledge of host-pathogen interactions. We have discussed Mevalonate kinase (MVK)-induced actin reorganization, modulation of signalling pathways and host cell functions. Our results show that LdMVK gains access to macrophage cytosol and induces actin ...
Source: Biochimie - December 20, 2023 Category: Biochemistry Authors: Tanvir Bamra Taj Shafi Sushmita Das Manjay Kumar Pradeep Das Source Type: research

New insights into the substrate specificity of cholesterol oxidases for more aware application
In this study, we have adopted an integrative approach, combining evolutionary analysis, classic enzymatic techniques and computational approaches, to elucidate the distinct features of four various ChOxes from Rhodococcussp. (RCO), Cromobacteriumsp. (CCO), Pseudomonas aeruginosa (PCO) and Burkhoderia cepacia (BCO). Comparative and evolutionary analysis of substrate-binding domain (SBD) and FAD-binding domain (FBD) helped to reveal the origin of ChOxes. We discovered that all forms of ChOxes had a common ancestor and that the structural differences evolved later during divergence. Further examination of amino acid variatio...
Source: Biochimie - December 17, 2023 Category: Biochemistry Authors: Michail Shapira Alexandra Dobysh Anastasia Liaudanskaya Hanna Aucharova Yaraslau Dzichenka Volha Bokuts Suzana Jovanovi ć-Šanta Aliaksey Yantsevich Source Type: research

Glutathione is required for growth and cadmium tolerance in the amphibian chytrid fungus, Batrachochytrium dendrobatidis
Biochimie. 2023 Dec 15:S0300-9084(23)00316-4. doi: 10.1016/j.biochi.2023.12.002. Online ahead of print.ABSTRACTBatrachochytrium dendrobatidis (Bd) is a lethal amphibian pathogen, partly due to its ability to evade the immune system of susceptible frog species. In many pathogenic fungi, the antioxidant glutathione is a virulence factor that helps neutralize oxidative stressors generated from host immune cells, as well as other environmental stressors such as heavy metals. The role of glutathione in stress tolerance in Bd has not been investigated. Here, we examine the changes in the glutathione pool after stress exposure an...
Source: Biochimie - December 17, 2023 Category: Biochemistry Authors: Rebecca J Webb Catherine Rush Lee Berger Lee F Skerratt Alexandra A Roberts Source Type: research

Counteracting diabetes-induced adipose tissue derived-stromal cell senescence
Biochimie. 2023 Dec 15:S0300-9084(23)00315-2. doi: 10.1016/j.biochi.2023.12.001. Online ahead of print.ABSTRACTAdipose tissue stromal cells (ADSCs) are prone to functional decline and senescence during metabolic disturbances. In diabetes mellitus (DM), the pathogenic microenvironment induces oxidative stress causing ADSCs to senesce. The senescence associated secretory phenotype (SASP) in turn drives disease progression. The pathogenesis of DM is thus both a cause and consequence of senescence. Therapeutically preventing the onset of senescence in ADSCs may play a significant role in preventing disease progression and dire...
Source: Biochimie - December 17, 2023 Category: Biochemistry Authors: Saiuree Govender Maria Jacoba Kruger Mari van de Vyver Source Type: research

New insights into the substrate specificity of cholesterol oxidases for more aware application
In this study, we have adopted an integrative approach, combining evolutionary analysis, classic enzymatic techniques and computational approaches, to elucidate the distinct features of four various ChOxes from Rhodococcussp. (RCO), Cromobacteriumsp. (CCO), Pseudomonas aeruginosa (PCO) and Burkhoderia cepacia (BCO). Comparative and evolutionary analysis of substrate-binding domain (SBD) and FAD-binding domain (FBD) helped to reveal the origin of ChOxes. We discovered that all forms of ChOxes had a common ancestor and that the structural differences evolved later during divergence. Further examination of amino acid variatio...
Source: Biochimie - December 17, 2023 Category: Biochemistry Authors: Michail Shapira Alexandra Dobysh Anastasia Liaudanskaya Hanna Aucharova Yaraslau Dzichenka Volha Bokuts Suzana Jovanovi ć-Šanta Aliaksey Yantsevich Source Type: research

Glutathione is required for growth and cadmium tolerance in the amphibian chytrid fungus, Batrachochytrium dendrobatidis
Biochimie. 2023 Dec 15:S0300-9084(23)00316-4. doi: 10.1016/j.biochi.2023.12.002. Online ahead of print.ABSTRACTBatrachochytrium dendrobatidis (Bd) is a lethal amphibian pathogen, partly due to its ability to evade the immune system of susceptible frog species. In many pathogenic fungi, the antioxidant glutathione is a virulence factor that helps neutralize oxidative stressors generated from host immune cells, as well as other environmental stressors such as heavy metals. The role of glutathione in stress tolerance in Bd has not been investigated. Here, we examine the changes in the glutathione pool after stress exposure an...
Source: Biochimie - December 17, 2023 Category: Biochemistry Authors: Rebecca J Webb Catherine Rush Lee Berger Lee F Skerratt Alexandra A Roberts Source Type: research

Counteracting diabetes-induced adipose tissue derived-stromal cell senescence
Biochimie. 2023 Dec 15:S0300-9084(23)00315-2. doi: 10.1016/j.biochi.2023.12.001. Online ahead of print.ABSTRACTAdipose tissue stromal cells (ADSCs) are prone to functional decline and senescence during metabolic disturbances. In diabetes mellitus (DM), the pathogenic microenvironment induces oxidative stress causing ADSCs to senesce. The senescence associated secretory phenotype (SASP) in turn drives disease progression. The pathogenesis of DM is thus both a cause and consequence of senescence. Therapeutically preventing the onset of senescence in ADSCs may play a significant role in preventing disease progression and dire...
Source: Biochimie - December 17, 2023 Category: Biochemistry Authors: Saiuree Govender Maria Jacoba Kruger Mari van de Vyver Source Type: research

New insights into the substrate specificity of cholesterol oxidases for more aware application
In this study, we have adopted an integrative approach, combining evolutionary analysis, classic enzymatic techniques and computational approaches, to elucidate the distinct features of four various ChOxes from Rhodococcussp. (RCO), Cromobacteriumsp. (CCO), Pseudomonas aeruginosa (PCO) and Burkhoderia cepacia (BCO). Comparative and evolutionary analysis of substrate-binding domain (SBD) and FAD-binding domain (FBD) helped to reveal the origin of ChOxes. We discovered that all forms of ChOxes had a common ancestor and that the structural differences evolved later during divergence. Further examination of amino acid variatio...
Source: Biochimie - December 17, 2023 Category: Biochemistry Authors: Michail Shapira Alexandra Dobysh Anastasia Liaudanskaya Hanna Aucharova Yaraslau Dzichenka Volha Bokuts Suzana Jovanovi ć-Šanta Aliaksey Yantsevich Source Type: research

Glutathione is required for growth and cadmium tolerance in the amphibian chytrid fungus, Batrachochytrium dendrobatidis
Biochimie. 2023 Dec 15:S0300-9084(23)00316-4. doi: 10.1016/j.biochi.2023.12.002. Online ahead of print.ABSTRACTBatrachochytrium dendrobatidis (Bd) is a lethal amphibian pathogen, partly due to its ability to evade the immune system of susceptible frog species. In many pathogenic fungi, the antioxidant glutathione is a virulence factor that helps neutralize oxidative stressors generated from host immune cells, as well as other environmental stressors such as heavy metals. The role of glutathione in stress tolerance in Bd has not been investigated. Here, we examine the changes in the glutathione pool after stress exposure an...
Source: Biochimie - December 17, 2023 Category: Biochemistry Authors: Rebecca J Webb Catherine Rush Lee Berger Lee F Skerratt Alexandra A Roberts Source Type: research