Biochemical and metabolic characterization of a G6PC2 inhibitor
Biochimie. 2024 Feb 29:S0300-9084(24)00050-6. doi: 10.1016/j.biochi.2024.02.012. Online ahead of print.ABSTRACTThree glucose-6-phosphatase catalytic subunits, that hydrolyze glucose-6-phosphate (G6P) to glucose and inorganic phosphate, have been identified, designated G6PC1-3, but only G6PC1 and G6PC2 have been implicated in the regulation of fasting blood glucose (FBG). Elevated FBG has been associated with multiple adverse clinical outcomes, including increased risk for type 2 diabetes and various cancers. Therefore, G6PC1 and G6PC2 inhibitors that lower FBG may be of prophylactic value for the prevention of multiple con...
Source: Biochimie - March 2, 2024 Category: Biochemistry Authors: Emily M Hawes Mohsin Rahim Zeinab Haratipour Abigail R Orun Margaret L O'Rourke James K Oeser Kwangho Kim Derek P Claxton Ray D Blind Jamey D Young Richard M O'Brien Source Type: research

Elucidating the pivotal role of TSPO in porphyrin-related cellular processes, in Bacillus cereus
In this study, we generated a ΔtspO mutant strain in B. cereus ATCC 14579 and assessed the impact of the absence of BcTSPO on cellular proteomics and physiological characteristics. The proteomic analysis revealed correlations between the lack of BcTSPO and the observed growth defects, increased oxygen consumption, ATP deficiency, heightened tryptophan catabolism, reduced motility, and impaired biofilm formation in the ΔtspO mutant strain. Our results also suggested that BcTSPO plays a crucial role in regulating intracellular levels of metabolites from the coproporphyrin-dependent branch of the heme biosynthetic pathway. ...
Source: Biochimie - February 29, 2024 Category: Biochemistry Authors: Catherine Duport Jean Armengaud Schmitt Caroline Morin Didier Lacapere Jean-Jacques Source Type: research

Elucidating the pivotal role of TSPO in porphyrin-related cellular processes, in Bacillus cereus
In this study, we generated a ΔtspO mutant strain in B. cereus ATCC 14579 and assessed the impact of the absence of BcTSPO on cellular proteomics and physiological characteristics. The proteomic analysis revealed correlations between the lack of BcTSPO and the observed growth defects, increased oxygen consumption, ATP deficiency, heightened tryptophan catabolism, reduced motility, and impaired biofilm formation in the ΔtspO mutant strain. Our results also suggested that BcTSPO plays a crucial role in regulating intracellular levels of metabolites from the coproporphyrin-dependent branch of the heme biosynthetic pathway. ...
Source: Biochimie - February 29, 2024 Category: Biochemistry Authors: Catherine Duport Jean Armengaud Schmitt Caroline Morin Didier Lacapere Jean-Jacques Source Type: research

Elucidating the pivotal role of TSPO in porphyrin-related cellular processes, in Bacillus cereus
In this study, we generated a ΔtspO mutant strain in B. cereus ATCC 14579 and assessed the impact of the absence of BcTSPO on cellular proteomics and physiological characteristics. The proteomic analysis revealed correlations between the lack of BcTSPO and the observed growth defects, increased oxygen consumption, ATP deficiency, heightened tryptophan catabolism, reduced motility, and impaired biofilm formation in the ΔtspO mutant strain. Our results also suggested that BcTSPO plays a crucial role in regulating intracellular levels of metabolites from the coproporphyrin-dependent branch of the heme biosynthetic pathway. ...
Source: Biochimie - February 29, 2024 Category: Biochemistry Authors: Catherine Duport Jean Armengaud Schmitt Caroline Morin Didier Lacapere Jean-Jacques Source Type: research

Benefits of rutin on mitochondrial function and inflammation in an aluminum-induced neurotoxicity rat model: Potential interest for the prevention of neurodegeneration
In this study, we aimed to investigate the potential of rutin, a naturally occurring plant bioactive molecule, to mitigate the neurotoxic effects induced by aluminum chloride (AlCl3). Over a period of 6 weeks, rats were intraperitoneally injected with AlCl3 at a weekly dose of 60 mg/kg, while rutin treatment was administered orally via gavage at a daily dose of 30 mg/kg. AlCl3 exposure resulted in a significant increase lipid peroxidation (LPO) by 316.24%, nitrate levels by 504.14%, and tumor necrosis factor-alpha (TNF-α) levels by 93.82% in brain mitochondria. Additionally, AlCl3 exposure led to a reduction in glutathion...
Source: Biochimie - February 26, 2024 Category: Biochemistry Authors: Khadidja Kessas Wafaa Lounis Zehor Chouari Anne Vejux G érard Lizard Omar Kharoubi Source Type: research

Characterization of SLA RNA promoter from dengue virus and its interaction with the viral non-structural NS5 protein
Biochimie. 2024 Feb 24:S0300-9084(24)00043-9. doi: 10.1016/j.biochi.2024.02.005. Online ahead of print.ABSTRACTThe Dengue virus (DENV) is the most significant arthropod-borne viral pathogen in humans with 400 million infections annually. DENV comprises four distinct serotypes (DENV-1 to -4) which complicates vaccine development. Any of the four serotypes can cause clinical illness but with distinctive infection dynamics. Variations in sequences identified within the four genomes induce structural differences in crucial RNA motifs that were suggested to be correlated to the degree of pathogenicity among DENV-1 to -4. In par...
Source: Biochimie - February 26, 2024 Category: Biochemistry Authors: Karl Brillet Marta Janczuk-Richter Amanda Poon Joanne Laukart-Bradley Eric Ennifar Isabelle Lebars Source Type: research

Benefits of rutin on mitochondrial function and inflammation in an aluminum-induced neurotoxicity rat model: Potential interest for the prevention of neurodegeneration
In this study, we aimed to investigate the potential of rutin, a naturally occurring plant bioactive molecule, to mitigate the neurotoxic effects induced by aluminum chloride (AlCl3). Over a period of 6 weeks, rats were intraperitoneally injected with AlCl3 at a weekly dose of 60 mg/kg, while rutin treatment was administered orally via gavage at a daily dose of 30 mg/kg. AlCl3 exposure resulted in a significant increase lipid peroxidation (LPO) by 316.24%, nitrate levels by 504.14%, and tumor necrosis factor-alpha (TNF-α) levels by 93.82% in brain mitochondria. Additionally, AlCl3 exposure led to a reduction in glutathion...
Source: Biochimie - February 26, 2024 Category: Biochemistry Authors: Khadidja Kessas Wafaa Lounis Zehor Chouari Anne Vejux G érard Lizard Omar Kharoubi Source Type: research

Characterization of SLA RNA promoter from dengue virus and its interaction with the viral non-structural NS5 protein
Biochimie. 2024 Feb 24:S0300-9084(24)00043-9. doi: 10.1016/j.biochi.2024.02.005. Online ahead of print.ABSTRACTThe Dengue virus (DENV) is the most significant arthropod-borne viral pathogen in humans with 400 million infections annually. DENV comprises four distinct serotypes (DENV-1 to -4) which complicates vaccine development. Any of the four serotypes can cause clinical illness but with distinctive infection dynamics. Variations in sequences identified within the four genomes induce structural differences in crucial RNA motifs that were suggested to be correlated to the degree of pathogenicity among DENV-1 to -4. In par...
Source: Biochimie - February 26, 2024 Category: Biochemistry Authors: Karl Brillet Marta Janczuk-Richter Amanda Poon Joanne Laukart-Bradley Eric Ennifar Isabelle Lebars Source Type: research

Benefits of rutin on mitochondrial function and inflammation in an aluminum-induced neurotoxicity rat model: Potential interest for the prevention of neurodegeneration
In this study, we aimed to investigate the potential of rutin, a naturally occurring plant bioactive molecule, to mitigate the neurotoxic effects induced by aluminum chloride (AlCl3). Over a period of 6 weeks, rats were intraperitoneally injected with AlCl3 at a weekly dose of 60 mg/kg, while rutin treatment was administered orally via gavage at a daily dose of 30 mg/kg. AlCl3 exposure resulted in a significant increase lipid peroxidation (LPO) by 316.24%, nitrate levels by 504.14%, and tumor necrosis factor-alpha (TNF-α) levels by 93.82% in brain mitochondria. Additionally, AlCl3 exposure led to a reduction in glutathion...
Source: Biochimie - February 26, 2024 Category: Biochemistry Authors: Khadidja Kessas Wafaa Lounis Zehor Chouari Anne Vejux G érard Lizard Omar Kharoubi Source Type: research

Characterization of SLA RNA promoter from dengue virus and its interaction with the viral non-structural NS5 protein
Biochimie. 2024 Feb 24:S0300-9084(24)00043-9. doi: 10.1016/j.biochi.2024.02.005. Online ahead of print.ABSTRACTThe Dengue virus (DENV) is the most significant arthropod-borne viral pathogen in humans with 400 million infections annually. DENV comprises four distinct serotypes (DENV-1 to -4) which complicates vaccine development. Any of the four serotypes can cause clinical illness but with distinctive infection dynamics. Variations in sequences identified within the four genomes induce structural differences in crucial RNA motifs that were suggested to be correlated to the degree of pathogenicity among DENV-1 to -4. In par...
Source: Biochimie - February 26, 2024 Category: Biochemistry Authors: Karl Brillet Marta Janczuk-Richter Amanda Poon Joanne Laukart-Bradley Eric Ennifar Isabelle Lebars Source Type: research

Pyridoxal kinase gene deletion leads to impaired growth, deranged redox metabolism and cell cycle arrest in Leishmania donovani
Biochimie. 2024 Feb 23:S0300-9084(24)00047-6. doi: 10.1016/j.biochi.2024.02.009. Online ahead of print.ABSTRACTPyridoxal kinase (PdxK) is a vitamin B6 salvage pathway enzyme which produces pyridoxal phosphate. We have investigated the impact of PdxK deletion in Leishmania donovani on parasite survivability, infectivity and cellular metabolism. LdPdxK mutants were generated by gene replacement strategy. All mutants showed significant reduction in growth in comparison to wild type. For PdxK mediated biochemical perturbations, only heterozygous mutants and complementation mutants were used as the growth of null mutants were c...
Source: Biochimie - February 25, 2024 Category: Biochemistry Authors: Pradyot Kumar Roy Anindita Paul Sandra Lalchhuanawmi Neerupudi Kishore Babu Sushma Singh Source Type: research

Pyridoxal kinase gene deletion leads to impaired growth, deranged redox metabolism and cell cycle arrest in Leishmania donovani
Biochimie. 2024 Feb 23:S0300-9084(24)00047-6. doi: 10.1016/j.biochi.2024.02.009. Online ahead of print.ABSTRACTPyridoxal kinase (PdxK) is a vitamin B6 salvage pathway enzyme which produces pyridoxal phosphate. We have investigated the impact of PdxK deletion in Leishmania donovani on parasite survivability, infectivity and cellular metabolism. LdPdxK mutants were generated by gene replacement strategy. All mutants showed significant reduction in growth in comparison to wild type. For PdxK mediated biochemical perturbations, only heterozygous mutants and complementation mutants were used as the growth of null mutants were c...
Source: Biochimie - February 25, 2024 Category: Biochemistry Authors: Pradyot Kumar Roy Anindita Paul Sandra Lalchhuanawmi Neerupudi Kishore Babu Sushma Singh Source Type: research

The 'Not-So-Famous Five' in tumorigenesis: tRNAs, tRNA fragments, and tRNA epitranscriptome in concert with AARSs and AIMPs
Biochimie. 2024 Feb 22:S0300-9084(24)00042-7. doi: 10.1016/j.biochi.2024.02.004. Online ahead of print.ABSTRACTRNA profiling studies have revealed that ∼75% of the human genome is transcribed to RNA but only a meagre fraction of it is translated to proteins. Majority of transcribed RNA constitute a specialized pool of non-coding RNAs. Human genome contains approximately 506 genes encoding a set of 51 different tRNAs, constituting a unique class of non-coding RNAs that not only have essential housekeeping functions as translator molecules during protein synthesis, but have numerous uncharted regulatory functions. Intrigui...
Source: Biochimie - February 24, 2024 Category: Biochemistry Authors: Sutapa Saha Biyas Mukherjee Proma Banerjee Debadrita Das Source Type: research

Dioclea violacea lectin inhibits tumorigenesis and tumor angiogenesis in vivo
This study evaluated the cytotoxic effect of DvL on tumor and normal cells using the mitochondrial activity reduction (MTT) assay, the carcinogenic and anti-carcinogenic activity by the epithelial tumor test (ETT) in Drosophila melanogaster, and the anti-angiogenic effect by the chick embryo chorioallantoic membrane (CAM) assay. Data demonstrated that DvL promoted strong selective cytotoxicity against tumor cell lines, especially A549 and S180 cells, whereas normal cell lines were weakly affected. Furthermore, DvL did not promote carcinogenesis in D. melanogaster at any concentration tested, but modulated DXR-induced carci...
Source: Biochimie - February 23, 2024 Category: Biochemistry Authors: Abel Vieira de Melo Bisneto Amanda Silva Fernandes L ívia do Carmo Silva Luana Santos Silva Diego Pereira de Ara újo Ivan Cerqueira Dos Santos Marcella da Rocha Melo Rom ério Rodrigues Dos Santos Silva Leonardo Pereira Franchi Clever Gomes Cardoso Elis Source Type: research

Dioclea violacea lectin inhibits tumorigenesis and tumor angiogenesis in vivo
This study evaluated the cytotoxic effect of DvL on tumor and normal cells using the mitochondrial activity reduction (MTT) assay, the carcinogenic and anti-carcinogenic activity by the epithelial tumor test (ETT) in Drosophila melanogaster, and the anti-angiogenic effect by the chick embryo chorioallantoic membrane (CAM) assay. Data demonstrated that DvL promoted strong selective cytotoxicity against tumor cell lines, especially A549 and S180 cells, whereas normal cell lines were weakly affected. Furthermore, DvL did not promote carcinogenesis in D. melanogaster at any concentration tested, but modulated DXR-induced carci...
Source: Biochimie - February 23, 2024 Category: Biochemistry Authors: Abel Vieira de Melo Bisneto Amanda Silva Fernandes L ívia do Carmo Silva Luana Santos Silva Diego Pereira de Ara újo Ivan Cerqueira Dos Santos Marcella da Rocha Melo Rom ério Rodrigues Dos Santos Silva Leonardo Pereira Franchi Clever Gomes Cardoso Elis Source Type: research