Dioclea violacea lectin inhibits tumorigenesis and tumor angiogenesis in vivo
This study evaluated the cytotoxic effect of DvL on tumor and normal cells using the mitochondrial activity reduction (MTT) assay, the carcinogenic and anti-carcinogenic activity by the epithelial tumor test (ETT) in Drosophila melanogaster, and the anti-angiogenic effect by the chick embryo chorioallantoic membrane (CAM) assay. Data demonstrated that DvL promoted strong selective cytotoxicity against tumor cell lines, especially A549 and S180 cells, whereas normal cell lines were weakly affected. Furthermore, DvL did not promote carcinogenesis in D. melanogaster at any concentration tested, but modulated DXR-induced carci...
Source: Biochimie - February 23, 2024 Category: Biochemistry Authors: Abel Vieira de Melo Bisneto Amanda Silva Fernandes L ívia do Carmo Silva Luana Santos Silva Diego Pereira de Ara újo Ivan Cerqueira Dos Santos Marcella da Rocha Melo Rom ério Rodrigues Dos Santos Silva Leonardo Pereira Franchi Clever Gomes Cardoso Elis Source Type: research
Dioclea violacea lectin inhibits tumorigenesis and tumor angiogenesis in vivo
This study evaluated the cytotoxic effect of DvL on tumor and normal cells using the mitochondrial activity reduction (MTT) assay, the carcinogenic and anti-carcinogenic activity by the epithelial tumor test (ETT) in Drosophila melanogaster, and the anti-angiogenic effect by the chick embryo chorioallantoic membrane (CAM) assay. Data demonstrated that DvL promoted strong selective cytotoxicity against tumor cell lines, especially A549 and S180 cells, whereas normal cell lines were weakly affected. Furthermore, DvL did not promote carcinogenesis in D. melanogaster at any concentration tested, but modulated DXR-induced carci...
Source: Biochimie - February 23, 2024 Category: Biochemistry Authors: Abel Vieira de Melo Bisneto Amanda Silva Fernandes L ívia do Carmo Silva Luana Santos Silva Diego Pereira de Ara újo Ivan Cerqueira Dos Santos Marcella da Rocha Melo Rom ério Rodrigues Dos Santos Silva Leonardo Pereira Franchi Clever Gomes Cardoso Elis Source Type: research
The cellular SFPQ protein as a positive factor in the HIV-1 integration
Biochimie. 2024 Feb 17:S0300-9084(24)00038-5. doi: 10.1016/j.biochi.2024.02.002. Online ahead of print.ABSTRACTThe cellular SFPQ protein is involved in several stages of the HIV-1 life cycle, but the detailed mechanism of its involvement is not yet fully understood. Here, the role of SFPQ in the early stages of HIV-1 replication has been studied. It is found that changes in the intracellular level of SFPQ affect the integration of viral DNA, but not reverse transcription, and SFPQ is a positive factor of integration. A study of the SFPQ interaction with HIV-1 integrase (IN) has revealed two diRGGX1-4 motifs in the N-termin...
Source: Biochimie - February 19, 2024 Category: Biochemistry Authors: Тatiana Kikhai Yulia Agapkina Maria Silkina Tatiana Prikazchikova Marina Gottikh Source Type: research
The cellular SFPQ protein as a positive factor in the HIV-1 integration
Biochimie. 2024 Feb 17:S0300-9084(24)00038-5. doi: 10.1016/j.biochi.2024.02.002. Online ahead of print.ABSTRACTThe cellular SFPQ protein is involved in several stages of the HIV-1 life cycle, but the detailed mechanism of its involvement is not yet fully understood. Here, the role of SFPQ in the early stages of HIV-1 replication has been studied. It is found that changes in the intracellular level of SFPQ affect the integration of viral DNA, but not reverse transcription, and SFPQ is a positive factor of integration. A study of the SFPQ interaction with HIV-1 integrase (IN) has revealed two diRGGX1-4 motifs in the N-termin...
Source: Biochimie - February 19, 2024 Category: Biochemistry Authors: Тatiana Kikhai Yulia Agapkina Maria Silkina Tatiana Prikazchikova Marina Gottikh Source Type: research
The cellular SFPQ protein as a positive factor in the HIV-1 integration
Biochimie. 2024 Feb 17:S0300-9084(24)00038-5. doi: 10.1016/j.biochi.2024.02.002. Online ahead of print.ABSTRACTThe cellular SFPQ protein is involved in several stages of the HIV-1 life cycle, but the detailed mechanism of its involvement is not yet fully understood. Here, the role of SFPQ in the early stages of HIV-1 replication has been studied. It is found that changes in the intracellular level of SFPQ affect the integration of viral DNA, but not reverse transcription, and SFPQ is a positive factor of integration. A study of the SFPQ interaction with HIV-1 integrase (IN) has revealed two diRGGX1-4 motifs in the N-termin...
Source: Biochimie - February 19, 2024 Category: Biochemistry Authors: Тatiana Kikhai Yulia Agapkina Maria Silkina Tatiana Prikazchikova Marina Gottikh Source Type: research
The cellular SFPQ protein as a positive factor in the HIV-1 integration
Biochimie. 2024 Feb 17:S0300-9084(24)00038-5. doi: 10.1016/j.biochi.2024.02.002. Online ahead of print.ABSTRACTThe cellular SFPQ protein is involved in several stages of the HIV-1 life cycle, but the detailed mechanism of its involvement is not yet fully understood. Here, the role of SFPQ in the early stages of HIV-1 replication has been studied. It is found that changes in the intracellular level of SFPQ affect the integration of viral DNA, but not reverse transcription, and SFPQ is a positive factor of integration. A study of the SFPQ interaction with HIV-1 integrase (IN) has revealed two diRGGX1-4 motifs in the N-termin...
Source: Biochimie - February 19, 2024 Category: Biochemistry Authors: Тatiana Kikhai Yulia Agapkina Maria Silkina Tatiana Prikazchikova Marina Gottikh Source Type: research
Renal ischemia and reperfusion impact the purinergic signaling in a vascular bed distant from the injured site
This study aimed to investigate the impact of AKI on purinergic components in mice aorta.MAIN METHODS: The kidney ischemia was achieved by the occlusion of the left kidney pedicle for 60 min, followed by reperfusion for 8 (IR8) and 15 (IR15) days. Renal function was assessed through biochemical assays, while gene expression levels were evaluated by RT-qPCR.KEY FINDINGS: Analyses of renal parameters showed renal remodeling through mass loss in the left kidney and hypertrophy of the right kidney in the IR15 group. Furthermore, after 15 days, local inflammation was evidenced in the aorta. Moreover, the aorta purinergic compon...
Source: Biochimie - February 15, 2024 Category: Biochemistry Authors: Jeferson Stabile Raquel Silva Neres-Santos Isabela Dorta Molina Hernandes Carolina Vict ória Cruz Junho Geovane Felippe Alves Isabella Cardoso Silva Marcela Sorelli Carneiro-Ramos Cristina Ribas F ürstenau Source Type: research
Renal ischemia and reperfusion impact the purinergic signaling in a vascular bed distant from the injured site
This study aimed to investigate the impact of AKI on purinergic components in mice aorta.MAIN METHODS: The kidney ischemia was achieved by the occlusion of the left kidney pedicle for 60 min, followed by reperfusion for 8 (IR8) and 15 (IR15) days. Renal function was assessed through biochemical assays, while gene expression levels were evaluated by RT-qPCR.KEY FINDINGS: Analyses of renal parameters showed renal remodeling through mass loss in the left kidney and hypertrophy of the right kidney in the IR15 group. Furthermore, after 15 days, local inflammation was evidenced in the aorta. Moreover, the aorta purinergic compon...
Source: Biochimie - February 15, 2024 Category: Biochemistry Authors: Jeferson Stabile Raquel Silva Neres-Santos Isabela Dorta Molina Hernandes Carolina Vict ória Cruz Junho Geovane Felippe Alves Isabella Cardoso Silva Marcela Sorelli Carneiro-Ramos Cristina Ribas F ürstenau Source Type: research
Renal ischemia and reperfusion impact the purinergic signaling in a vascular bed distant from the injured site
This study aimed to investigate the impact of AKI on purinergic components in mice aorta.MAIN METHODS: The kidney ischemia was achieved by the occlusion of the left kidney pedicle for 60 min, followed by reperfusion for 8 (IR8) and 15 (IR15) days. Renal function was assessed through biochemical assays, while gene expression levels were evaluated by RT-qPCR.KEY FINDINGS: Analyses of renal parameters showed renal remodeling through mass loss in the left kidney and hypertrophy of the right kidney in the IR15 group. Furthermore, after 15 days, local inflammation was evidenced in the aorta. Moreover, the aorta purinergic compon...
Source: Biochimie - February 15, 2024 Category: Biochemistry Authors: Jeferson Stabile Raquel Silva Neres-Santos Isabela Dorta Molina Hernandes Carolina Vict ória Cruz Junho Geovane Felippe Alves Isabella Cardoso Silva Marcela Sorelli Carneiro-Ramos Cristina Ribas F ürstenau Source Type: research
Renal ischemia and reperfusion impact the purinergic signaling in a vascular bed distant from the injured site
This study aimed to investigate the impact of AKI on purinergic components in mice aorta.MAIN METHODS: The kidney ischemia was achieved by the occlusion of the left kidney pedicle for 60 min, followed by reperfusion for 8 (IR8) and 15 (IR15) days. Renal function was assessed through biochemical assays, while gene expression levels were evaluated by RT-qPCR.KEY FINDINGS: Analyses of renal parameters showed renal remodeling through mass loss in the left kidney and hypertrophy of the right kidney in the IR15 group. Furthermore, after 15 days, local inflammation was evidenced in the aorta. Moreover, the aorta purinergic compon...
Source: Biochimie - February 15, 2024 Category: Biochemistry Authors: Jeferson Stabile Raquel Silva Neres-Santos Isabela Dorta Molina Hernandes Carolina Vict ória Cruz Junho Geovane Felippe Alves Isabella Cardoso Silva Marcela Sorelli Carneiro-Ramos Cristina Ribas F ürstenau Source Type: research
Dynamics and metabolic effects of intestinal gases in healthy humans
Biochimie. 2024 Feb 5;221:81-90. doi: 10.1016/j.biochi.2024.02.001. Online ahead of print.ABSTRACTMany living beings use exogenous and/or endogenous gases to attain evolutionary benefits. We make a comprehensive assessment of one of the major gaseous reservoirs in the human body, i.e., the bowel, providing extensive data that may serve as reference for future studies. We assess the intestinal gases in healthy humans, including their volume, composition, source and local distribution in proximal as well as distal gut. We analyse each one of the most abundant intestinal gases including nitrogen, oxygen, nitric oxide, carbon ...
Source: Biochimie - February 7, 2024 Category: Biochemistry Authors: None Arturo Tozzi Raffaele Minella Source Type: research
Multi-oligomeric and catalytically compromised serine acetyltransferase and cysteine regulatory complex of Mycobacterium tuberculosis
This study fills the gap and provides experimental evidence for the presence of MtCRC and a non-canonical multi-oligomeric MtSAT. We employed multiple analytical methods to characterize the oligomeric and kinetic properties of MtSAT and MtCRC. Results show that MtSAT, lacking >75 N-terminal amino acids exists in three different assembly states; trimer, hexamer, and dodecamer, compared to the single hexameric state of SAT of other bacteria. While hexamers display the highest catalytic turnover, the trimer is the least active. The predominance of trimers at low physiologically relevant concentrations suggests that MtSAT d...
Source: Biochimie - February 4, 2024 Category: Biochemistry Authors: Rahisuddin R Payal Thakur Narender Kumar Neha Saini Shrijta Banerjee Ravi Pratap Singh Madhuri Patel S Kumaran Source Type: research
Cell cycle and mitosis progression during ZIKA virus infection: The viral non-structural protein NS5 as a master regulator of the APC/cyclosome?
Biochimie. 2024 Feb 1;221:75-80. doi: 10.1016/j.biochi.2024.01.016. Online ahead of print.ABSTRACTAlterations in cell cycle regulation contribute to Zika virus (ZIKV)-associated pathogenesis and may have implications for the development of therapeutic avenues. As a matter of fact, ZIKV alters cell cycle progression at multiple stages, including G1, S, G2, and M phases. During a cell cycle, the progression of mitosis is particularly controlled to avoid any abnormalities in cell division. In this regard, the critical metaphase-anaphase transition is triggered by the activation of anaphase-promoting complex/cyclosome (APC/C) ...
Source: Biochimie - February 2, 2024 Category: Biochemistry Authors: Gr égorie Lebeau Mathilde Hoareau S ébastien Rivière Daed El Safadi Christine Robert Da Silva Pascale Krejbich-Trotot Wildriss Viranaicken Source Type: research
Role of the Escherichia coli FocA and FocB formate channels in controlling proton/potassium fluxes and hydrogen production during osmotic stress in energy-limited, stationary phase fermenting cells
Biochimie. 2024 Feb 1;221:91-98. doi: 10.1016/j.biochi.2024.01.017. Online ahead of print.ABSTRACTEscherichia coli FocA and FocB formate channels export formate or import it for further disproportionation by the formate hydrogenlyase (FHL) complex to H2 and CO2. Here, we show that under pH and osmotic stress FocA and FocB play important roles in regulating proton and potassium fluxes and couple this with H2 production in stationary-phase cells. Using whole-cell assays with glucose as electron donor, a focB mutant showed a 50 % decrease in VH2, while N'N'-dicyclohexylcarbodiimide (DCCD) treatment of osmotically stressed cel...
Source: Biochimie - February 2, 2024 Category: Biochemistry Authors: A Babayan A Vassilian A Poladyan K Trchounian Source Type: research
Should the standard model of cellular energy metabolism be reconsidered? Possible coupling between the pentose phosphate pathway, glycolysis and extra-mitochondrial oxidative phosphorylation
Biochimie. 2024 Feb 1;221:99-109. doi: 10.1016/j.biochi.2024.01.018. Online ahead of print.ABSTRACTThe process of cellular respiration occurs for energy production through catabolic reactions, generally with glucose as the first process step. In the present work, we introduce a novel concept for understanding this process, based on our conclusion that glucose metabolism is coupled to the pentose phosphate pathway (PPP) and extra-mitochondrial oxidative phosphorylation in a closed-loop process. According to the current standard model of glycolysis, glucose is first converted to glucose 6-phosphate (glucose 6-P) and then to ...
Source: Biochimie - February 2, 2024 Category: Biochemistry Authors: Alessandro Maria Morelli Felix Scholkmann Source Type: research
