Desmin and its molecular chaperone, the αB-crystallin: How post-translational modifications modulate their functions in heart and skeletal muscles?
Biochimie. 2023 Oct 10:S0300-9084(23)00256-0. doi: 10.1016/j.biochi.2023.10.002. Online ahead of print.ABSTRACTMaintenance of the highly organized striated muscle tissue requires a cell-wide dynamic network through protein-protein interactions providing an effective mechanochemical integrator of morphology and function. Through a continuous and complex trans-cytoplasmic network, desmin intermediate filaments ensure this essential role in heart and in skeletal muscle. Besides their role in the maintenance of cell shape and architecture (permitting contractile activity efficiency and conferring resistance towards mechanical ...
Source: Biochimie - October 12, 2023 Category: Biochemistry Authors: Charlotte Claeyssen Nathan Bulangalire Bruno Bastide Onnik Agbulut Caroline Cieniewski-Bernard Source Type: research

Desmin and its molecular chaperone, the αB-crystallin: How post-translational modifications modulate their functions in heart and skeletal muscles?
Biochimie. 2023 Oct 10:S0300-9084(23)00256-0. doi: 10.1016/j.biochi.2023.10.002. Online ahead of print.ABSTRACTMaintenance of the highly organized striated muscle tissue requires a cell-wide dynamic network through protein-protein interactions providing an effective mechanochemical integrator of morphology and function. Through a continuous and complex trans-cytoplasmic network, desmin intermediate filaments ensure this essential role in heart and in skeletal muscle. Besides their role in the maintenance of cell shape and architecture (permitting contractile activity efficiency and conferring resistance towards mechanical ...
Source: Biochimie - October 12, 2023 Category: Biochemistry Authors: Charlotte Claeyssen Nathan Bulangalire Bruno Bastide Onnik Agbulut Caroline Cieniewski-Bernard Source Type: research

Flexibility of active center affects thermostability and activity of Penicillium canescens xylanase E
Biochimie. 2023 Oct 9:S0300-9084(23)00258-4. doi: 10.1016/j.biochi.2023.10.004. Online ahead of print.ABSTRACTXylanases are used in several industrial applications, such as feed additives, the bleaching of pulp and paper, and the production of bread, food, and drinks. Xylanases are required to remain active after heat treatment at 80-90 °C for 30 s to several minutes due to the conditions of feed pelleting. Also, xylanases need to be active at 60-70 °C for several hours while bleaching of pulp and paper or manufacturing of bread, food, and drinks is performed. Xylanases of the glycoside hydrolase family GH10 are good can...
Source: Biochimie - October 11, 2023 Category: Biochemistry Authors: Anna Dotsenko Igor Sinelnikov Aleksandra Rozhkova Ivan Zorov Arkady Sinitsyn Source Type: research

Alpha hemolysin of E. coli induces hemolysis of human erythrocytes independently of toxin interaction with membrane proteins
Biochimie. 2023 Oct 9:S0300-9084(23)00271-7. doi: 10.1016/j.biochi.2023.10.008. Online ahead of print.ABSTRACTAlpha hemolysin (HlyA) is a hemolytic and cytotoxic protein secreted by uropathogenic strains of E. coli. The role of glycophorins (GPs) as putative receptors for HlyA binding to red blood cells (RBCs) has been debated. Experiments using anti-GPA/GPB antibodies and a GPA-specific epitope nanobody to block HlyA-GP binding on hRBCs, showed no effect on hemolytic activity. Similarly, the hemolysis induced by HlyA remained unaffected when hRBCs from a GPAnull/GPBnull variant were used. Surface Plasmon Resonance experim...
Source: Biochimie - October 11, 2023 Category: Biochemistry Authors: Luc ía Cané Nicol ás Andrés Saffioti Sandrine Genetet Mar ía Antonieta Daza Millone Mariano A Ostuni Pablo J Schwarzbaum Isabelle Mouro-Chanteloup Vanesa Herlax Source Type: research

Flexibility of active center affects thermostability and activity of Penicillium canescens xylanase E
Biochimie. 2023 Oct 9:S0300-9084(23)00258-4. doi: 10.1016/j.biochi.2023.10.004. Online ahead of print.ABSTRACTXylanases are used in several industrial applications, such as feed additives, the bleaching of pulp and paper, and the production of bread, food, and drinks. Xylanases are required to remain active after heat treatment at 80-90 °C for 30 s to several minutes due to the conditions of feed pelleting. Also, xylanases need to be active at 60-70 °C for several hours while bleaching of pulp and paper or manufacturing of bread, food, and drinks is performed. Xylanases of the glycoside hydrolase family GH10 are good can...
Source: Biochimie - October 11, 2023 Category: Biochemistry Authors: Anna Dotsenko Igor Sinelnikov Aleksandra Rozhkova Ivan Zorov Arkady Sinitsyn Source Type: research

Alpha hemolysin of E. coli induces hemolysis of human erythrocytes independently of toxin interaction with membrane proteins
Biochimie. 2023 Oct 9:S0300-9084(23)00271-7. doi: 10.1016/j.biochi.2023.10.008. Online ahead of print.ABSTRACTAlpha hemolysin (HlyA) is a hemolytic and cytotoxic protein secreted by uropathogenic strains of E. coli. The role of glycophorins (GPs) as putative receptors for HlyA binding to red blood cells (RBCs) has been debated. Experiments using anti-GPA/GPB antibodies and a GPA-specific epitope nanobody to block HlyA-GP binding on hRBCs, showed no effect on hemolytic activity. Similarly, the hemolysis induced by HlyA remained unaffected when hRBCs from a GPAnull/GPBnull variant were used. Surface Plasmon Resonance experim...
Source: Biochimie - October 11, 2023 Category: Biochemistry Authors: Luc ía Cané Nicol ás Andrés Saffioti Sandrine Genetet Mar ía Antonieta Daza Millone Mariano A Ostuni Pablo J Schwarzbaum Isabelle Mouro-Chanteloup Vanesa Herlax Source Type: research

Structure and function of Campylobacter jejuni polynucleotide phosphorylase (PNPase): Insights into the role of this RNase in pathogenicity
Biochimie. 2023 Oct 6:S0300-9084(23)00268-7. doi: 10.1016/j.biochi.2023.10.006. Online ahead of print.ABSTRACTRibonucleases are in charge of the processing, degradation and quality control of all cellular transcripts, which makes them crucial factors in RNA regulation. This post-transcriptional regulation allows bacteria to promptly react to different stress conditions and growth phase transitions, and also to produce the required virulence factors in pathogenic bacteria. Campylobacter jejuni is the main responsible for human gastroenteritis in the world. In this foodborne pathogen, exoribonuclease PNPase (CjPNP) is essent...
Source: Biochimie - October 8, 2023 Category: Biochemistry Authors: C átia Bárria Diogo Athayde Guillem Hernandez Leonor Fonseca Jorge Casinhas Tiago N Cordeiro Margarida Archer Cec ília M Arraiano Jos é A Brito Rute G Matos Source Type: research

A U2 snRNP-specific protein, U2A', is involved in stress response and drug resistance in Cryptococcus deneoformans
In this study, we identified a U2A' homologous protein as a component of the cryptococcal U2 snRNP, which was encoded by the LEA1 gene. Using the "suicide" CRISPR-Cas9 tool, we deleted the LEA1 gene in C. deneoformans JEC21 strain and obtained the disruption mutant lea1Δ. The mutant showed a hypersensitivity to 0.03 % sodium dodecyl sulfate, as well as disordered chitin distribution in cell wall observed with Calcofluor White staining, which collectively illustrated the function of U2A' in maintenance of cell wall integrity. Further examination showed that lea1Δ displayed a decreased tolerance to lower or elevated temper...
Source: Biochimie - October 8, 2023 Category: Biochemistry Authors: Lan Ma Xueqing Zhang Chenxi Li Xiaoyu Ma Xuan Zhao Xueru Zhao Ping Zhang Xudong Zhu Source Type: research

Coordination between human DNA polymerase β and apurinic/apyrimidinic endonuclease 1 in the course of DNA repair
Biochimie. 2023 Oct 6:S0300-9084(23)00270-5. doi: 10.1016/j.biochi.2023.10.007. Online ahead of print.ABSTRACTCoordination of enzymatic activities in the course of base excision repair (BER) is essential to ensure complete repair of damaged bases. Two major mechanisms underlying the coordination of BER are known today: the "passing the baton" model and a model of preassembled stable multiprotein repair complexes called "repairosomes." In this work, we aimed to elucidate the coordination between human apurinic/apyrimidinic (AP) endonuclease APE1 and DNA polymerase Polβ in BER through studying an impact of APE1 on Polβ-cat...
Source: Biochimie - October 8, 2023 Category: Biochemistry Authors: Artemiy S Bakman Stanislav S Boichenko Aleksandra A Kuznetsova Alexander A Ishchenko Murat Saparbaev Nikita A Kuznetsov Source Type: research

Structure and function of Campylobacter jejuni polynucleotide phosphorylase (PNPase): Insights into the role of this RNase in pathogenicity
Biochimie. 2023 Oct 6:S0300-9084(23)00268-7. doi: 10.1016/j.biochi.2023.10.006. Online ahead of print.ABSTRACTRibonucleases are in charge of the processing, degradation and quality control of all cellular transcripts, which makes them crucial factors in RNA regulation. This post-transcriptional regulation allows bacteria to promptly react to different stress conditions and growth phase transitions, and also to produce the required virulence factors in pathogenic bacteria. Campylobacter jejuni is the main responsible for human gastroenteritis in the world. In this foodborne pathogen, exoribonuclease PNPase (CjPNP) is essent...
Source: Biochimie - October 8, 2023 Category: Biochemistry Authors: C átia Bárria Diogo Athayde Guillem Hernandez Leonor Fonseca Jorge Casinhas Tiago N Cordeiro Margarida Archer Cec ília M Arraiano Jos é A Brito Rute G Matos Source Type: research

A U2 snRNP-specific protein, U2A', is involved in stress response and drug resistance in Cryptococcus deneoformans
In this study, we identified a U2A' homologous protein as a component of the cryptococcal U2 snRNP, which was encoded by the LEA1 gene. Using the "suicide" CRISPR-Cas9 tool, we deleted the LEA1 gene in C. deneoformans JEC21 strain and obtained the disruption mutant lea1Δ. The mutant showed a hypersensitivity to 0.03 % sodium dodecyl sulfate, as well as disordered chitin distribution in cell wall observed with Calcofluor White staining, which collectively illustrated the function of U2A' in maintenance of cell wall integrity. Further examination showed that lea1Δ displayed a decreased tolerance to lower or elevated temper...
Source: Biochimie - October 8, 2023 Category: Biochemistry Authors: Lan Ma Xueqing Zhang Chenxi Li Xiaoyu Ma Xuan Zhao Xueru Zhao Ping Zhang Xudong Zhu Source Type: research

Coordination between human DNA polymerase β and apurinic/apyrimidinic endonuclease 1 in the course of DNA repair
Biochimie. 2023 Oct 6:S0300-9084(23)00270-5. doi: 10.1016/j.biochi.2023.10.007. Online ahead of print.ABSTRACTCoordination of enzymatic activities in the course of base excision repair (BER) is essential to ensure complete repair of damaged bases. Two major mechanisms underlying the coordination of BER are known today: the "passing the baton" model and a model of preassembled stable multiprotein repair complexes called "repairosomes." In this work, we aimed to elucidate the coordination between human apurinic/apyrimidinic (AP) endonuclease APE1 and DNA polymerase Polβ in BER through studying an impact of APE1 on Polβ-cat...
Source: Biochimie - October 8, 2023 Category: Biochemistry Authors: Artemiy S Bakman Stanislav S Boichenko Aleksandra A Kuznetsova Alexander A Ishchenko Murat Saparbaev Nikita A Kuznetsov Source Type: research

Structure and function of Campylobacter jejuni polynucleotide phosphorylase (PNPase): Insights into the role of this RNase in pathogenicity
Biochimie. 2023 Oct 6:S0300-9084(23)00268-7. doi: 10.1016/j.biochi.2023.10.006. Online ahead of print.ABSTRACTRibonucleases are in charge of the processing, degradation and quality control of all cellular transcripts, which makes them crucial factors in RNA regulation. This post-transcriptional regulation allows bacteria to promptly react to different stress conditions and growth phase transitions, and also to produce the required virulence factors in pathogenic bacteria. Campylobacter jejuni is the main responsible for human gastroenteritis in the world. In this foodborne pathogen, exoribonuclease PNPase (CjPNP) is essent...
Source: Biochimie - October 8, 2023 Category: Biochemistry Authors: C átia Bárria Diogo Athayde Guillem Hernandez Leonor Fonseca Jorge Casinhas Tiago N Cordeiro Margarida Archer Cec ília M Arraiano Jos é A Brito Rute G Matos Source Type: research

A U2 snRNP-specific protein, U2A', is involved in stress response and drug resistance in Cryptococcus deneoformans
In this study, we identified a U2A' homologous protein as a component of the cryptococcal U2 snRNP, which was encoded by the LEA1 gene. Using the "suicide" CRISPR-Cas9 tool, we deleted the LEA1 gene in C. deneoformans JEC21 strain and obtained the disruption mutant lea1Δ. The mutant showed a hypersensitivity to 0.03 % sodium dodecyl sulfate, as well as disordered chitin distribution in cell wall observed with Calcofluor White staining, which collectively illustrated the function of U2A' in maintenance of cell wall integrity. Further examination showed that lea1Δ displayed a decreased tolerance to lower or elevated temper...
Source: Biochimie - October 8, 2023 Category: Biochemistry Authors: Lan Ma Xueqing Zhang Chenxi Li Xiaoyu Ma Xuan Zhao Xueru Zhao Ping Zhang Xudong Zhu Source Type: research

Coordination between human DNA polymerase β and apurinic/apyrimidinic endonuclease 1 in the course of DNA repair
Biochimie. 2023 Oct 6:S0300-9084(23)00270-5. doi: 10.1016/j.biochi.2023.10.007. Online ahead of print.ABSTRACTCoordination of enzymatic activities in the course of base excision repair (BER) is essential to ensure complete repair of damaged bases. Two major mechanisms underlying the coordination of BER are known today: the "passing the baton" model and a model of preassembled stable multiprotein repair complexes called "repairosomes." In this work, we aimed to elucidate the coordination between human apurinic/apyrimidinic (AP) endonuclease APE1 and DNA polymerase Polβ in BER through studying an impact of APE1 on Polβ-cat...
Source: Biochimie - October 8, 2023 Category: Biochemistry Authors: Artemiy S Bakman Stanislav S Boichenko Aleksandra A Kuznetsova Alexander A Ishchenko Murat Saparbaev Nikita A Kuznetsov Source Type: research