Dynamics and metabolic effects of intestinal gases in healthy humans
Biochimie. 2024 Feb 5;221:81-90. doi: 10.1016/j.biochi.2024.02.001. Online ahead of print.ABSTRACTMany living beings use exogenous and/or endogenous gases to attain evolutionary benefits. We make a comprehensive assessment of one of the major gaseous reservoirs in the human body, i.e., the bowel, providing extensive data that may serve as reference for future studies. We assess the intestinal gases in healthy humans, including their volume, composition, source and local distribution in proximal as well as distal gut. We analyse each one of the most abundant intestinal gases including nitrogen, oxygen, nitric oxide, carbon ...
Source: Biochimie - February 7, 2024 Category: Biochemistry Authors: None Arturo Tozzi Raffaele Minella Source Type: research
Multi-oligomeric and catalytically compromised serine acetyltransferase and cysteine regulatory complex of Mycobacterium tuberculosis
This study fills the gap and provides experimental evidence for the presence of MtCRC and a non-canonical multi-oligomeric MtSAT. We employed multiple analytical methods to characterize the oligomeric and kinetic properties of MtSAT and MtCRC. Results show that MtSAT, lacking >75 N-terminal amino acids exists in three different assembly states; trimer, hexamer, and dodecamer, compared to the single hexameric state of SAT of other bacteria. While hexamers display the highest catalytic turnover, the trimer is the least active. The predominance of trimers at low physiologically relevant concentrations suggests that MtSAT d...
Source: Biochimie - February 4, 2024 Category: Biochemistry Authors: Rahisuddin R Payal Thakur Narender Kumar Neha Saini Shrijta Banerjee Ravi Pratap Singh Madhuri Patel S Kumaran Source Type: research
Cell cycle and mitosis progression during ZIKA virus infection: The viral non-structural protein NS5 as a master regulator of the APC/cyclosome?
Biochimie. 2024 Feb 1;221:75-80. doi: 10.1016/j.biochi.2024.01.016. Online ahead of print.ABSTRACTAlterations in cell cycle regulation contribute to Zika virus (ZIKV)-associated pathogenesis and may have implications for the development of therapeutic avenues. As a matter of fact, ZIKV alters cell cycle progression at multiple stages, including G1, S, G2, and M phases. During a cell cycle, the progression of mitosis is particularly controlled to avoid any abnormalities in cell division. In this regard, the critical metaphase-anaphase transition is triggered by the activation of anaphase-promoting complex/cyclosome (APC/C) ...
Source: Biochimie - February 2, 2024 Category: Biochemistry Authors: Gr égorie Lebeau Mathilde Hoareau S ébastien Rivière Daed El Safadi Christine Robert Da Silva Pascale Krejbich-Trotot Wildriss Viranaicken Source Type: research
Role of the Escherichia coli FocA and FocB formate channels in controlling proton/potassium fluxes and hydrogen production during osmotic stress in energy-limited, stationary phase fermenting cells
Biochimie. 2024 Feb 1;221:91-98. doi: 10.1016/j.biochi.2024.01.017. Online ahead of print.ABSTRACTEscherichia coli FocA and FocB formate channels export formate or import it for further disproportionation by the formate hydrogenlyase (FHL) complex to H2 and CO2. Here, we show that under pH and osmotic stress FocA and FocB play important roles in regulating proton and potassium fluxes and couple this with H2 production in stationary-phase cells. Using whole-cell assays with glucose as electron donor, a focB mutant showed a 50 % decrease in VH2, while N'N'-dicyclohexylcarbodiimide (DCCD) treatment of osmotically stressed cel...
Source: Biochimie - February 2, 2024 Category: Biochemistry Authors: A Babayan A Vassilian A Poladyan K Trchounian Source Type: research
Should the standard model of cellular energy metabolism be reconsidered? Possible coupling between the pentose phosphate pathway, glycolysis and extra-mitochondrial oxidative phosphorylation
Biochimie. 2024 Feb 1;221:99-109. doi: 10.1016/j.biochi.2024.01.018. Online ahead of print.ABSTRACTThe process of cellular respiration occurs for energy production through catabolic reactions, generally with glucose as the first process step. In the present work, we introduce a novel concept for understanding this process, based on our conclusion that glucose metabolism is coupled to the pentose phosphate pathway (PPP) and extra-mitochondrial oxidative phosphorylation in a closed-loop process. According to the current standard model of glycolysis, glucose is first converted to glucose 6-phosphate (glucose 6-P) and then to ...
Source: Biochimie - February 2, 2024 Category: Biochemistry Authors: Alessandro Maria Morelli Felix Scholkmann Source Type: research
Isoprenylcysteine carboxyl methyltransferase (ICMT) promotes invadopodia formation and metastasis in cancer cells
Biochimie. 2024 Jan 30:S0300-9084(24)00033-6. doi: 10.1016/j.biochi.2024.01.015. Online ahead of print.ABSTRACTIsoprenyl cysteine carboxyl methyltransferase (ICMT) catalyzes the last step of the prenylation pathway. Previously, we found that high ICMT levels enhance tumorigenesis in vivo and that its expression is repressed by the p53 tumor suppressor. Based on evidence suggesting that some ICMT substrates affect invasive traits, we wondered if this enzyme may promote metastasis. In this work, we found that ICMT overexpression enhanced lung metastasis in vivo. Accordingly, ICMT overexpression also promoted cellular functio...
Source: Biochimie - February 1, 2024 Category: Biochemistry Authors: Carla Borini Etichetti Evelyn Arel Zalazar Carolina Di Benedetto Nabila Cocordano Sabrina Valente Silvio Bicciato Mauricio Menacho-M árquez Mar ía Cecilia Larocca Javier Girardini Source Type: research
Identification and characterisation of a novel interaction between oestrogen receptor alpha and FOXP2
This study could serve as a foundation for uncovering the basis of sexual dimorphism in speech and language development and related disorders and potentially offers an alternate for targeted cancer therapies.PMID:38296156 | DOI:10.1016/j.biochi.2024.01.014 (Source: Biochimie)
Source: Biochimie - January 31, 2024 Category: Biochemistry Authors: Aasiya Lakhi Sylvia Fanucchi Source Type: research
Among the recombinant TSPOs, the BcTSPO
Biochimie. 2024 Jan 25:S0300-9084(24)00029-4. doi: 10.1016/j.biochi.2024.01.011. Online ahead of print.ABSTRACTOverexpression of recombinant Bacillus cereus TSPO (BcTSPO) in E. coli bacteria leads to its recovery with a bound hemin both in bacterial membrane (MB) and inclusion bodies (IB). Unlike mouse TSPO, BcTSPO purified in SDS detergent from IB is well structured and can bind various ligands such as high-affinity PK 11195, protoporphyrin IX (PPIX) and δ-aminolevulinic acid (ALA). For each of the three ligands, 1H-15N HSQC titration NMR experiments suggest that different amino acids of BcTSPO binding cavity are involve...
Source: Biochimie - January 27, 2024 Category: Biochemistry Authors: Leeyah Issop Luminita Duma Stephanie Finet Olivier Lequin Jean-Jacques Lacap ère Source Type: research
Translocator protein (TSPO) ligands attenuate mitophagy deficits in the SH-SY5Y cellular model of Alzheimer's disease via the autophagy adaptor P62
Biochimie. 2024 Jan 26:S0300-9084(24)00030-0. doi: 10.1016/j.biochi.2024.01.012. Online ahead of print.ABSTRACTMitochondrial dysfunction has been widely implicated in the pathogenesis of Alzheimer's disease (AD), with accumulation of damaged and dysfunctional mitochondria occurring early in the disease. Mitophagy, which governs mitochondrial turnover and quality control, is impaired in the AD brain, and strategies aimed at enhancing mitophagy have been identified as promising therapeutic targets. The translocator protein (TSPO) is an outer mitochondrial membrane protein that is upregulated in AD, and ligands targeting TSPO...
Source: Biochimie - January 27, 2024 Category: Biochemistry Authors: Lauren H Fairley Amandine Grimm Steffen A Herff Anne Eckert Source Type: research
The rise of RNA: From fundamental research to therapeutic applications
Biochimie. 2024 Feb;217:1-2. doi: 10.1016/j.biochi.2024.01.005.NO ABSTRACTPMID:38272605 | DOI:10.1016/j.biochi.2024.01.005 (Source: Biochimie)
Source: Biochimie - January 25, 2024 Category: Biochemistry Authors: Marie Sissler Fabien Darfeuille Source Type: research
Amplifying PCR productivity and environmental sustainability through shortened cycling protocols
This study aimed to systemically evaluate the effect of reduced PCR cycling parameters on amplicon production. The 1466bp fragment from the 16S rRNA gene present in low-, medium- and high-CG bacteria was amplified using three commercially available PCR master mixes. The shortest cycling parameters required to successfully amplify the 16S fragment from all bacteria and master mixes comprised 30-cycles of 5 s denaturation, 25 s annealing, and 25 s extension. While all produced an amplicon with sufficient yield to enable downstream sequence analysis, the PCRBIO Ultra Mix in conjunction with the shortened parameters was found ...
Source: Biochimie - January 23, 2024 Category: Biochemistry Authors: Matthew Pedlar Matthew J Emery Philip J Warburton Source Type: research
Deciphering the Akt1-HuD interaction in HuD-mediated neuronal differentiation
Biochimie. 2024 Jan 18;221:20-26. doi: 10.1016/j.biochi.2024.01.010. Online ahead of print.ABSTRACTThe RNA-binding protein HuD/ELAVL4 is essential for neuronal development and synaptic plasticity by governing various post-transcriptional processes of target mRNAs, including stability, translation, and localization. We previously showed that the linker region and poly(A)-binding domain of HuD play a pivotal role in promoting translation and inducing neurite outgrowth. In addition, we found that HuD interacts exclusively with the active form of Akt1, through the linker region. Although this interaction is essential for neuri...
Source: Biochimie - January 20, 2024 Category: Biochemistry Authors: Hikari Nishisaka Takumi Tomohiro Kako Fukuzumi Akira Fukao Yoshinori Funakami Toshinobu Fujiwara Source Type: research
Discovery and characterization of the α-amylases cDNAs from Enchytraeus albidus shed light on the evolution of "Enchytraeus-Eisenia type" Amy homologs in Annelida
This study investigates α-amylases, major digestive enzymes responsible for hydrolyzing starch and similar polysaccharides into sugars, in Enchytraeus albidus. Genetic data related to α-amylases is currently lacking for the family Enchytraeidae but also for the entire Annelida. To detect and identify coding sequences of the expressed α-amylase genes in COI-monohaplotype culture (PL-A strain) of E. albidus, we used classical "gene fishing" and transcriptomic approaches. We also compared coding sequence variants of α-amylase retrieved from transcriptomic data related to freeze-tolerant strains. Our results reveal that E....
Source: Biochimie - January 19, 2024 Category: Biochemistry Authors: Łukasz Gajda Agata Daszkowska-Golec Piotr Świątek Source Type: research
Dynamic modulation of transthylakoid electric potential by chloroplast ATP synthases
Biochimie. 2024 Jan 13;221:27-37. doi: 10.1016/j.biochi.2024.01.007. Online ahead of print.ABSTRACTThe light-induced transthylakoid membrane potential (ΔΨm) can function as a driving force to help catalyzing the formation of ATP molecules, proving a tight connection between ΔΨm and the ATP synthase. Naturally, a question can be raised on the effects of altered functioning of ATP synthases on regulating ΔΨm, which is attractive in the area of photosynthetic research. Lots of findings, when making efforts of solving this difficulty, can offer an in-depth understanding into the mechanism behind. However, the functional ...
Source: Biochimie - January 15, 2024 Category: Biochemistry Authors: Hui Lyu Yong-Song Zuo Source Type: research
Cell-penetrating peptide and cationic liposomes mediated siRNA delivery to arrest growth of chronic myeloid leukemia cells in vitro
Biochimie. 2024 Jan 10;221:1-12. doi: 10.1016/j.biochi.2024.01.006. Online ahead of print.ABSTRACTGene silencing through RNA interference (RNAi) is a promising therapeutic approach for a wide range of disorders, including cancer. Non-viral gene therapy, using specific siRNAs against BCR-ABL1, can be a supportive or alternative measure to traditional chronic myeloid leukemia (CML) tyrosine kinase inhibitor (TKIs) therapies, given the prevalence of clinical TKI resistance. The main challenge for such approaches remains the development of the effective delivery system for siRNA tailored to the specific disease model. The purp...
Source: Biochimie - January 12, 2024 Category: Biochemistry Authors: Vera Vysochinskaya Yana Zabrodskaya Olesya Dovbysh Anton Emelyanov Vladimir Klimenko Nikolay Knyazev Ivan Terterov Marya Egorova Alexey Bogdanov Michael Maslov Andrey Vasin Michael Dubina Source Type: research