Functional involvement of a conserved motif in the middle region of the human ribosomal protein eL42 in translation
Biochimie. 2023 Sep 14:S0300-9084(23)00219-5. doi: 10.1016/j.biochi.2023.09.010. Online ahead of print.ABSTRACTRibosomal protein eL42 (formerly known as L36A), a small protein of the large (60S) subunit of the eukaryotic ribosome, is a component of its exit (E) site. The residue K53 of this protein resides within the motif QSGYGGQTK mainly conserved in eukaryotes, and it is located in the immediate vicinity of the CCA-terminus of the ribosome-bound tRNA in the hybrid P/E state. To examine the role of this eL42 motif in translation, we obtained HEK293T cells producing the wild-type FLAG-tagged protein or its mutant forms wi...
Source: Biochimie - September 16, 2023 Category: Biochemistry Authors: Konstantin N Bulygin Alexey A Malygin Dmitri M Graifer Source Type: research

Structure of glyceraldehyde-3-phosphate dehydrogenase from Paracoccidioides lutzii in complex with an aldonic sugar acid
Biochimie. 2023 Sep 12:S0300-9084(23)00240-7. doi: 10.1016/j.biochi.2023.09.013. Online ahead of print.ABSTRACTThe pathogen Paracoccidioides lutzii (Pb01) is found in South America countries Colombia, Ecuador, Venezuela and Brazil, especially in the central, west, and north regions of the latter. It belongs to the Ajellomycetaceae family, Onygenales order, and is typically thermodimorphic, presenting yeast cells when it grows in animal tissues, but mycelia when in the environment, where it produces the infectious propagule. Moreover, this fungus is one of the etiologic agents of Paracoccidioidomycosis (PCM), the most impor...
Source: Biochimie - September 14, 2023 Category: Biochemistry Authors: Jonathan Heiler Hern ández-Prieto Viviane Paula Martini Jorge Iulek Source Type: research

Structure of glyceraldehyde-3-phosphate dehydrogenase from Paracoccidioides lutzii in complex with an aldonic sugar acid
Biochimie. 2023 Sep 12:S0300-9084(23)00240-7. doi: 10.1016/j.biochi.2023.09.013. Online ahead of print.ABSTRACTThe pathogen Paracoccidioides lutzii (Pb01) is found in South America countries Colombia, Ecuador, Venezuela and Brazil, especially in the central, west, and north regions of the latter. It belongs to the Ajellomycetaceae family, Onygenales order, and is typically thermodimorphic, presenting yeast cells when it grows in animal tissues, but mycelia when in the environment, where it produces the infectious propagule. Moreover, this fungus is one of the etiologic agents of Paracoccidioidomycosis (PCM), the most impor...
Source: Biochimie - September 14, 2023 Category: Biochemistry Authors: Jonathan Heiler Hern ández-Prieto Viviane Paula Martini Jorge Iulek Source Type: research

Structure of glyceraldehyde-3-phosphate dehydrogenase from Paracoccidioides lutzii in complex with an aldonic sugar acid
Biochimie. 2023 Sep 12:S0300-9084(23)00240-7. doi: 10.1016/j.biochi.2023.09.013. Online ahead of print.ABSTRACTThe pathogen Paracoccidioides lutzii (Pb01) is found in South America countries Colombia, Ecuador, Venezuela and Brazil, especially in the central, west, and north regions of the latter. It belongs to the Ajellomycetaceae family, Onygenales order, and is typically thermodimorphic, presenting yeast cells when it grows in animal tissues, but mycelia when in the environment, where it produces the infectious propagule. Moreover, this fungus is one of the etiologic agents of Paracoccidioidomycosis (PCM), the most impor...
Source: Biochimie - September 14, 2023 Category: Biochemistry Authors: Jonathan Heiler Hern ández-Prieto Viviane Paula Martini Jorge Iulek Source Type: research

Antiplatelet mechanism of a subtilisin-like serine protease from Solanum tuberosum (StSBTc-3)
Biochimie. 2023 Sep 11:S0300-9084(23)00238-9. doi: 10.1016/j.biochi.2023.09.011. Online ahead of print.ABSTRACTThe aims of this study are to characterize the antiplatelet activity of StSBTc-3, a potato serine protease with fibrino(geno) lytic activity, and to provide information on its mechanism of action. The results obtained show that StSBTc-3 inhibits clot retraction and prevents platelet aggregation induced by thrombin, convulxin, and A23187. Platelet aggregation inhibition occurs in a dose-dependent manner and is not affected by inactivation of StSBTc-3 with the inhibitor of serine proteases phenylmethylsulfonyl fluor...
Source: Biochimie - September 13, 2023 Category: Biochemistry Authors: Alfonso Pepe Florencia Rocio Tito Maria Gabriela Guevara Source Type: research

Isolation of the AccCDK8 gene of Apis cerana cerana and its functional analysis under pesticide and heavy metal stress
Biochimie. 2023 Sep 11:S0300-9084(23)00239-0. doi: 10.1016/j.biochi.2023.09.012. Online ahead of print.ABSTRACTEnvironmental pollution has gained negative attention in recent years. The pesticides and heavy metals are top list of environmental toxicants directly endangering the survival and development of Apis cerana cerana. Cyclin-dependent kinases (CDKs) are heteromeric serine/threonine kinases that participate in cell cycle regulation and have a vital role in pesticide and heavy metal stress in Apis cerana cerana. In this experiment, we filtered out CDK8 gene from Apis cerana cerana (AccCDK8) and investigated its functi...
Source: Biochimie - September 13, 2023 Category: Biochemistry Authors: Jing Li Dezheng Guo Jinhao Bai Hongfang Wang Chen Wang Ying Wang Xingqi Guo Baohua Xu Zhenguo Liu Source Type: research

Dissolved metal ions and mineral-liposome hybrid systems: Underlying interactions, synthesis, and characterization
Biochimie. 2023 Sep 10:S0300-9084(23)00218-3. doi: 10.1016/j.biochi.2023.09.009. Online ahead of print.ABSTRACTLiposomes are versatile lipid-based vesicles with interesting physicochemical properties, making them excellent candidates for interdisciplinary applications in the medicinal, biological, and environmental sciences. The synthesis of mineral-liposome hybrid systems lends normally inert vesicles with the catalytic, magnetic, electrical, and optical properties of the integrated mineral species. Such applications require an understanding of the physicochemical interactions between organic molecules and inorganic cryst...
Source: Biochimie - September 12, 2023 Category: Biochemistry Authors: Nil Gaudu Orion Farr Georges Ona-Nguema Simon Duval Source Type: research

Dissolved metal ions and mineral-liposome hybrid systems: Underlying interactions, synthesis, and characterization
Biochimie. 2023 Sep 10:S0300-9084(23)00218-3. doi: 10.1016/j.biochi.2023.09.009. Online ahead of print.ABSTRACTLiposomes are versatile lipid-based vesicles with interesting physicochemical properties, making them excellent candidates for interdisciplinary applications in the medicinal, biological, and environmental sciences. The synthesis of mineral-liposome hybrid systems lends normally inert vesicles with the catalytic, magnetic, electrical, and optical properties of the integrated mineral species. Such applications require an understanding of the physicochemical interactions between organic molecules and inorganic cryst...
Source: Biochimie - September 12, 2023 Category: Biochemistry Authors: Nil Gaudu Orion Farr Georges Ona-Nguema Simon Duval Source Type: research

Targeting of CRISPR-Cas12a crRNAs into human mitochondria
Biochimie. 2023 Sep 8:S0300-9084(23)00215-8. doi: 10.1016/j.biochi.2023.09.006. Online ahead of print.ABSTRACTMitochondrial gene editing holds great promise as a therapeutic approach for mitochondrial diseases caused by mutations in the mitochondrial DNA (mtDNA). Current strategies focus on reducing mutant mtDNA heteroplasmy levels through targeted cleavage or base editing. However, the delivery of editing components into mitochondria remains a challenge. Here we investigate the import of CRISPR-Cas12a system guide RNAs (crRNAs) into human mitochondria and study the structural requirements for this process by northern blot...
Source: Biochimie - September 10, 2023 Category: Biochemistry Authors: Natalia Nikitchina Egor Ulashchik Vadim Shmanai Anne-Marie Heckel Ivan Tarassov Ilya Mazunin Nina Entelis Source Type: research

Targeting of CRISPR-Cas12a crRNAs into human mitochondria
Biochimie. 2023 Sep 8:S0300-9084(23)00215-8. doi: 10.1016/j.biochi.2023.09.006. Online ahead of print.ABSTRACTMitochondrial gene editing holds great promise as a therapeutic approach for mitochondrial diseases caused by mutations in the mitochondrial DNA (mtDNA). Current strategies focus on reducing mutant mtDNA heteroplasmy levels through targeted cleavage or base editing. However, the delivery of editing components into mitochondria remains a challenge. Here we investigate the import of CRISPR-Cas12a system guide RNAs (crRNAs) into human mitochondria and study the structural requirements for this process by northern blot...
Source: Biochimie - September 10, 2023 Category: Biochemistry Authors: Natalia Nikitchina Egor Ulashchik Vadim Shmanai Anne-Marie Heckel Ivan Tarassov Ilya Mazunin Nina Entelis Source Type: research

Targeting of CRISPR-Cas12a crRNAs into human mitochondria
Biochimie. 2023 Sep 8:S0300-9084(23)00215-8. doi: 10.1016/j.biochi.2023.09.006. Online ahead of print.ABSTRACTMitochondrial gene editing holds great promise as a therapeutic approach for mitochondrial diseases caused by mutations in the mitochondrial DNA (mtDNA). Current strategies focus on reducing mutant mtDNA heteroplasmy levels through targeted cleavage or base editing. However, the delivery of editing components into mitochondria remains a challenge. Here we investigate the import of CRISPR-Cas12a system guide RNAs (crRNAs) into human mitochondria and study the structural requirements for this process by northern blot...
Source: Biochimie - September 10, 2023 Category: Biochemistry Authors: Natalia Nikitchina Egor Ulashchik Vadim Shmanai Anne-Marie Heckel Ivan Tarassov Ilya Mazunin Nina Entelis Source Type: research

A novel SPE-UPLC-MS/MS-based assay for the selective, simultaneous quantification of xylosyltransferase-I and -II activity
Biochimie. 2023 Sep 7:S0300-9084(23)00217-1. doi: 10.1016/j.biochi.2023.09.008. Online ahead of print.ABSTRACTXylosyltransferase-I and -II (XT-I, -II) possess a central role during the glycosylation of proteoglycans (PGs). They catalyze the formation of a O-glycosidic bond between the xylosyl residue of uridinediphosphate-xylose and the core protein of a PG. Thereafter, three following glycosyltransferases lead to the generation of a tetrasaccharide linker, which connects the PG core protein to the respective glycosaminoglycan. The selective quantification of XT-I and XT-II activity is of biological and clinical interest d...
Source: Biochimie - September 9, 2023 Category: Biochemistry Authors: Anika Kleine Matthias K ühle Joachim Kuhn Thanh-Diep Ly Vanessa Schmidt Isabel Faust-Hinse Cornelius Knabbe Bastian Fischer Source Type: research

Unsaturated fatty acid synthesis in bacteria: Mechanisms and regulation of canonical and remarkably noncanonical pathways
Biochimie. 2023 Sep 6:S0300-9084(23)00216-X. doi: 10.1016/j.biochi.2023.09.007. Online ahead of print.ABSTRACTUnsaturated phospholipid acyl chains are required for membrane function in most bacteria. The double bonds of the cis monoenoic chains arise by two distinct pathways depending on whether oxygen is required. The oxygen-independent pathway (traditionally called the anaerobic pathway) introduces the cis double bond by isomerization of the trans double bond intermediate of the fatty acid elongation cycle. Double bond isomerization occurs at an intermediate chain length (e.g., C10) and the isomerization product is elong...
Source: Biochimie - September 8, 2023 Category: Biochemistry Authors: John E Cronan Source Type: research

Effects of biophysical membrane properties on recognition of phosphatidylserine, or phosphatidylinositol 4-phosphate by lipid biosensors LactC2, or P4M
Biochimie. 2023 Sep 6:S0300-9084(23)00212-2. doi: 10.1016/j.biochi.2023.09.003. Online ahead of print.ABSTRACTLipid biosensors are molecular tools used both in vivo and in vitro applications, capable of selectively detecting specific types of lipids in biological membranes. However, despite their extensive use, there is a lack of systematic characterization of their binding properties in various membrane conditions. The purpose of this study was to investigate the impact of membrane properties, such as fluidity and membrane charge, on the sensitivity of two lipid biosensors, LactC2 and P4M, to their target lipids, phosphat...
Source: Biochimie - September 8, 2023 Category: Biochemistry Authors: Andrea Eisenreichov á Jana Humpol íčková Bartosz R óżycki Evzen Boura Alena Koukalov á Source Type: research

Unsaturated fatty acid synthesis in bacteria: Mechanisms and regulation of canonical and remarkably noncanonical pathways
Biochimie. 2023 Sep 6:S0300-9084(23)00216-X. doi: 10.1016/j.biochi.2023.09.007. Online ahead of print.ABSTRACTUnsaturated phospholipid acyl chains are required for membrane function in most bacteria. The double bonds of the cis monoenoic chains arise by two distinct pathways depending on whether oxygen is required. The oxygen-independent pathway (traditionally called the anaerobic pathway) introduces the cis double bond by isomerization of the trans double bond intermediate of the fatty acid elongation cycle. Double bond isomerization occurs at an intermediate chain length (e.g., C10) and the isomerization product is elong...
Source: Biochimie - September 8, 2023 Category: Biochemistry Authors: John E Cronan Source Type: research