Compensational role between cathepsins
Biochimie. 2024 Apr 23:S0300-9084(24)00085-3. doi: 10.1016/j.biochi.2024.04.010. Online ahead of print.ABSTRACTCathepsins, a family of lysosomal peptidases, play a crucial role in maintaining cellular homeostasis by regulating protein turnover and degradation as well as many specific regulatory actions that are important for proper cell function and human health. Alterations in the activity and expression of cathepsins have been observed in many diseases such as cancer, inflammation, neurodegenerative disorders, bone remodelling-related conditions and others. These changes are not exclusively harmful, but rather appear to ...
Source: Biochimie - April 25, 2024 Category: Biochemistry Authors: Ur ša Pečar Fonović Janko Kos Ana Mitrovi ć Source Type: research
Enhanced structure/function of mTSPO translocator in lipid:surfactant mixed micelles
In conclusion, mixed lipid:surfactant micelles open new possibilities for the stabilization of membrane proteins and for their study in solution in a more biomimetic amphiphilic environment.PMID:38663457 | DOI:10.1016/j.biochi.2024.04.008 (Source: Biochimie)
Source: Biochimie - April 25, 2024 Category: Biochemistry Authors: Christelle Saade Alexandre Pozza Fran çoise Bonnete St éphanie Finet Viviane Lutz-Bueno Mark D Tully Paloma F Varela Jean-Jacques Lacapere Sophie Combet Source Type: research
Mesenchymal Stem Cells in Autoimmune Disease: A Systematic Review and Meta-Analysis of Pre-Clinical Studies
Biochimie. 2024 Apr 22:S0300-9084(24)00084-1. doi: 10.1016/j.biochi.2024.04.009. Online ahead of print.ABSTRACTMesenchymal Stem Cells (MSCs) are of interest in the clinic because of their immunomodulation capabilities, capacity to act upstream of inflammation, and ability to sense metabolic environments. In standard physiologic conditions, they play a role in maintaining the homeostasis of tissues and organs; however, there is evidence that they can contribute to some autoimmune diseases. Gaining a deeper understanding of the factors that transition MSCs from their physiological function to a pathological role in their nat...
Source: Biochimie - April 24, 2024 Category: Biochemistry Authors: Hailey N Swain Parker D Boyce Bradley A Bromet Kaiden Barozinksy Lacy Hance Dakota Shields Gayla R Olbricht Julie A Semon Source Type: research
Advances in nuclear proteostasis of metazoans
Biochimie. 2024 Apr 18:S0300-9084(24)00081-6. doi: 10.1016/j.biochi.2024.04.006. Online ahead of print.ABSTRACTThe proteostasis network and associated protein quality control (PQC) mechanisms ensure proteome functionality and are essential for cell survival. A distinctive feature of eukaryotic cells is their high degree of compartmentalization, requiring specific and adapted proteostasis networks for each compartment. The nucleus, essential for maintaining the integrity of genetic information and gene transcription, is one such compartment. While PQC mechanisms have been investigated for decades in the cytoplasm and the en...
Source: Biochimie - April 20, 2024 Category: Biochemistry Authors: Julia Buggiani Thierry Meinnel Carmela Giglione Fr édéric Frottin Source Type: research
Effect of metformin in hypothalamic astrocytes from an immunocompromised mice model
Biochimie. 2024 Apr 18:S0300-9084(24)00080-4. doi: 10.1016/j.biochi.2024.04.005. Online ahead of print.ABSTRACTAstrocytes are glial cells that play key roles in neuroinflammation, which is a common feature in diabetic encephalopathy and aging process. Metformin is an antidiabetic compound that shows neuroprotective properties, including in inflammatory models, but astroglial signaling pathways involved are still poorly known. Interferons α/β are cytokines that participate in antiviral responses and the lack of their signaling increases susceptible to viral infections. Here, we investigated the effects of metformin on ast...
Source: Biochimie - April 20, 2024 Category: Biochemistry Authors: Larissa Daniele Bobermin Daniele Schauren da Costa Aline Daniel Moreira de Moraes Vanessa Fernanda da Silva Giancarlo Tomazzoni de Oliveira Patr ícia Sesterheim Ana Carolina Tramontina Luiz Augusto Basso Guilhian Leipnitz Andr é Quincozes-Santos Carlos- Source Type: research
Advances in nuclear proteostasis of metazoans
Biochimie. 2024 Apr 18:S0300-9084(24)00081-6. doi: 10.1016/j.biochi.2024.04.006. Online ahead of print.ABSTRACTThe proteostasis network and associated protein quality control (PQC) mechanisms ensure proteome functionality and are essential for cell survival. A distinctive feature of eukaryotic cells is their high degree of compartmentalization, requiring specific and adapted proteostasis networks for each compartment. The nucleus, essential for maintaining the integrity of genetic information and gene transcription, is one such compartment. While PQC mechanisms have been investigated for decades in the cytoplasm and the en...
Source: Biochimie - April 20, 2024 Category: Biochemistry Authors: Julia Buggiani Thierry Meinnel Carmela Giglione Fr édéric Frottin Source Type: research
Effect of metformin in hypothalamic astrocytes from an immunocompromised mice model
Biochimie. 2024 Apr 18:S0300-9084(24)00080-4. doi: 10.1016/j.biochi.2024.04.005. Online ahead of print.ABSTRACTAstrocytes are glial cells that play key roles in neuroinflammation, which is a common feature in diabetic encephalopathy and aging process. Metformin is an antidiabetic compound that shows neuroprotective properties, including in inflammatory models, but astroglial signaling pathways involved are still poorly known. Interferons α/β are cytokines that participate in antiviral responses and the lack of their signaling increases susceptible to viral infections. Here, we investigated the effects of metformin on ast...
Source: Biochimie - April 20, 2024 Category: Biochemistry Authors: Larissa Daniele Bobermin Daniele Schauren da Costa Aline Daniel Moreira de Moraes Vanessa Fernanda da Silva Giancarlo Tomazzoni de Oliveira Patr ícia Sesterheim Ana Carolina Tramontina Luiz Augusto Basso Guilhian Leipnitz Andr é Quincozes-Santos Carlos- Source Type: research
CpG methylation changes in human mesenchymal and neural stem cells in response to in vitro niche modifications
We examined CpG (5mC) site methylation patterns using Illumina Infinium 850K EPIC arrays in hNSC-H9, hMSCs and hMSC-IN cultures with HSPG agonist heparin at early and late phases of growth. We identified key regulatory CpG sites in syndecans (SDC2; SDC4) that potentially regulate gene expression in monolayers. Unique hMSC-IN hypomethylation in glypicans (GPC3; GPC4) underscore their significance in neural lineages with Sulfatase 1 and 2 (SULF1 &2) CpG methylation changes potentially driving the neurogenic shift. hMSC-INs methylation levels at SULF1 CpG sites and SULF2:cg25401628 were more closely aligned with hNSC-H9 c...
Source: Biochimie - April 19, 2024 Category: Biochemistry Authors: Martina Gyimesi Lotta E Oikari Chieh Yu Heidi G Sutherland Dale R Nyholt Lyn R Griffiths Andre J Van Wijnen Rachel K Okolicsanyi Larisa M Haupt Source Type: research
Impact of MG132 induced-proteotoxic stress on αB-crystallin and desmin phosphorylation and O-GlcNAcylation and their partition towards cytoskeleton
Biochimie. 2024 Apr 16:S0300-9084(24)00079-8. doi: 10.1016/j.biochi.2024.04.004. Online ahead of print.ABSTRACTSmall Heat Shock Proteins are considered as the first line of defense when proteostasis fails. Among them, αB-crystallin is expressed in striated muscles in which it interacts with desmin intermediate filaments to stabilize them, maintaining cytoskeleton's integrity and muscular functionalities. Desmin is a key actor for muscle health; its targeting by αB-crystallin is thus crucial, especially in stress conditions. αB-crystallin is phosphorylated and O-GlcNAcylated. Its phosphorylation increases consecutively t...
Source: Biochimie - April 18, 2024 Category: Biochemistry Authors: Nathan Bulangalire Charlotte Claeyssen Onnik Agbulut Caroline Cieniewski-Bernard Source Type: research
Impact of MG132 induced-proteotoxic stress on αB-crystallin and desmin phosphorylation and O-GlcNAcylation and their partition towards cytoskeleton
Biochimie. 2024 Apr 16:S0300-9084(24)00079-8. doi: 10.1016/j.biochi.2024.04.004. Online ahead of print.ABSTRACTSmall Heat Shock Proteins are considered as the first line of defense when proteostasis fails. Among them, αB-crystallin is expressed in striated muscles in which it interacts with desmin intermediate filaments to stabilize them, maintaining cytoskeleton's integrity and muscular functionalities. Desmin is a key actor for muscle health; its targeting by αB-crystallin is thus crucial, especially in stress conditions. αB-crystallin is phosphorylated and O-GlcNAcylated. Its phosphorylation increases consecutively t...
Source: Biochimie - April 18, 2024 Category: Biochemistry Authors: Nathan Bulangalire Charlotte Claeyssen Onnik Agbulut Caroline Cieniewski-Bernard Source Type: research
PA28 γ, the ring that makes tumors invisible to the immune system?
Biochimie. 2024 Apr 15:S0300-9084(24)00078-6. doi: 10.1016/j.biochi.2024.04.003. Online ahead of print.ABSTRACTPA28γ is a proteasomal interactor whose main and most known function is to stimulate the hydrolytic activity of the 20S proteasome independently of ubiquitin and ATP. Unlike its two paralogues, PA28α and PA28β, PA28γ is largely present in the nuclear compartment and plays pivotal functions in important pathways such as cellular division, apoptosis, neoplastic transformation, chromatin structure and organization, fertility, lipid metabolism, and DNA repair mechanisms. Although it is known that a substantial fra...
Source: Biochimie - April 17, 2024 Category: Biochemistry Authors: Paolo Cascio Source Type: research
Kallikrein-related peptidase's significance in Alzheimer's disease pathogenesis: A comprehensive survey
Biochimie. 2024 Apr 10:S0300-9084(24)00076-2. doi: 10.1016/j.biochi.2024.04.001. Online ahead of print.ABSTRACTAlzheimer's disease (AD) and related dementias constitute an important global health challenge. Detailed understanding of the multiple molecular mechanisms underlying their pathogenesis constitutes a clue for the management of the disease. Kallikrein-related peptidases (KLKs), a lead family of serine proteases, have emerged as potential biomarkers and therapeutic targets in the context of AD and associated cognitive decline. Hence, KLKs were proposed to display multifaceted impacts influencing various aspects of n...
Source: Biochimie - April 12, 2024 Category: Biochemistry Authors: Ril ès Boumali Laureline Urli Meriem Naim Feryel Soualmia Kiyoka Kinugawa Isabelle Petropoulos Chahrazade El Amri Source Type: research
NADPH oxidase-generated reactive oxygen species are involved in estradiol 17 ß-d-glucuronide-induced cholestasis
Biochimie. 2024 Apr 10;223:41-53. doi: 10.1016/j.biochi.2024.04.002. Online ahead of print.ABSTRACTThe endogenous metabolite of estradiol, estradiol 17β-D-glucuronide (E17G), is considered the main responsible of the intrahepatic cholestasis of pregnancy. E17G alters the activity of canalicular transporters through a signaling pathway-dependent cellular internalization, phenomenon that was attributed to oxidative stress in different cholestatic conditions. However, there are no reports involving oxidative stress in E17G-induced cholestasis, representing this the aim of our work. Using polarized hepatocyte cultures, we sho...
Source: Biochimie - April 12, 2024 Category: Biochemistry Authors: Gimena Salas Alen A Litta Anabela C Medeot Virginia S Schuck Romina B Andermatten Gisel S Miszczuk Nadia Ciriaci Ma Valeria Razori Ismael R Barosso Enrique J S ánchez Pozzi Marcelo G Roma Cecilia L Basiglio Fernando A Crocenzi Source Type: research
Kallikrein-related peptidase's significance in Alzheimer's disease pathogenesis: A comprehensive survey
Biochimie. 2024 Apr 10:S0300-9084(24)00076-2. doi: 10.1016/j.biochi.2024.04.001. Online ahead of print.ABSTRACTAlzheimer's disease (AD) and related dementias constitute an important global health challenge. Detailed understanding of the multiple molecular mechanisms underlying their pathogenesis constitutes a clue for the management of the disease. Kallikrein-related peptidases (KLKs), a lead family of serine proteases, have emerged as potential biomarkers and therapeutic targets in the context of AD and associated cognitive decline. Hence, KLKs were proposed to display multifaceted impacts influencing various aspects of n...
Source: Biochimie - April 12, 2024 Category: Biochemistry Authors: Ril ès Boumali Laureline Urli Meriem Naim Feryel Soualmia Kiyoka Kinugawa Isabelle Petropoulos Chahrazade El Amri Source Type: research
NADPH OXIDASE-GENERATED REACTIVE OXYGEN SPECIES ARE INVOLVED IN ESTRADIOL 17 ß-d-GLUCURONIDE-INDUCED CHOLESTASIS
Biochimie. 2024 Apr 10:S0300-9084(24)00077-4. doi: 10.1016/j.biochi.2024.04.002. Online ahead of print.ABSTRACTThe endogenous metabolite of estradiol, estradiol 17β-D-glucuronide (E17G), is considered the main responsible of the intrahepatic cholestasis of pregnancy. E17G alters the activity of canalicular transporters through a signaling pathway-dependent cellular internalization, phenomenon that was attributed to oxidative stress in different cholestatic conditions. However, there are no reports involving oxidative stress in E17G-induced cholestasis, representing this the aim of our work. Using polarized hepatocyte cult...
Source: Biochimie - April 12, 2024 Category: Biochemistry Authors: Gimena Salas Alen A Litta Anabela C Medeot Virginia Schuck Romina B Andermatten Gisel S Miszczuk Nadia Ciriaci Ma Valeria Razori Ismael R Barosso Enrique J S ánchez Pozzi Marcelo G Roma Cecilia L Basiglio Fernando A Crocenzi Source Type: research
