The 5-HT1A receptor: Signaling to behavior.
Abstract The 5-HT1A receptor is highly expressed both in 5-HT neurons as a presynaptic inhibitory autoreceptor, and in many brain regions innervated by 5-HT as a post-synaptic heteroreceptor. This review examines the signaling of 5-HT1A receptors to regulate 5-HT activity and behavior. Initial findings in heterologous cell systems, neuronal cell lines, neurons, and in vivo show that the 5-HT1A receptor is a Gi/o-coupled receptor that signals to the canonical pathway of inhibition of adenylyl cyclase (AC). However, new neuron-specific pathways and their roles in neuronal function have been uncovered. 5-HT1A re...
Source: Biochimie - May 14, 2019 Category: Biochemistry Authors: Albert PR, Vahid-Ansari F Tags: Biochimie Source Type: research

Insights into the structure, function and stability of bordonein-L, the first L-amino acid oxidase from Crotalus durissus terrificus snake venom.
Abstract Snake venom L-amino acid oxidases (svLAAOs) are an interesting class of enzymes with important biological activities. Their participation in key metabolic processes, including pathological disorders, suggest that svLAAOs are potential lead compounds in drug discovery. However, their short-term stability defies their applications. This paper describes the stability studies together with functional and structural characterization of the LAAO bordonein-L. It has 498 amino acid residues, one N-glycosylation site and two disulfide bonds, revealed by high-resolution MS/MS. Molecular modeling approach showed its...
Source: Biochimie - May 9, 2019 Category: Biochemistry Authors: Wiezel GA, Rustiguel JK, Morgenstern D, Zoccal KF, Faccioli LH, Nonato MC, Ueberheide B, Arantes EC Tags: Biochimie Source Type: research

SnoRNAs and the emerging class of sdRNAs: multifaceted players in oncogenesis.
Abstract Small nucleolar RNAs are generally involved in the modification of target ribosomal RNAs. Other possible functions recently emerged with the discovery of the novel class of snoRNA-derived RNAs or sdRNAs. Since then, additional data has revealed the involvement of both snoRNAs and sdRNAs in tumorigenesis. After briefly introducing snoRNA families and functions, this mini-review summarises recently acquired knowledge on snoRNA-related mechanisms associated with cancer. Despite the rapid increase in the number of studies, exactly how snoRNAs lead to cancer remains unclear. However, exciting new research is p...
Source: Biochimie - May 9, 2019 Category: Biochemistry Authors: Abel Y, Rederstorff M Tags: Biochimie Source Type: research

Characterization of bacterial acylpeptidyl-oligopeptidase.
Abstract Acylpeptidyl-oligopeptidase (AOP), which has been recently identified as a novel enzyme in a periodontopathic bacterium, Porphyromonas gingivalis, removes di- and tri-peptides from N-terminally acylated polypeptides, with a preference for hydrophobic P1-position amino acid residues. To validate enzymatic properties of AOP, characteristics of two bacterial orthologues from Bacteroides dorei (BdAOP), a Gram-negative intestinal rod, and Lysinibacillus sphaericus (LsAOP), a Gram-positive soil rod, were determined. Like P. gingivalis AOP (PgAOP), two orthologues more efficiently hydrolyzed N-terminal acylated ...
Source: Biochimie - May 9, 2019 Category: Biochemistry Authors: Nemoto TK, Ono T, Kobayakawa T, Ohara-Nemoto Y Tags: Biochimie Source Type: research

Catalpol attenuates cardiomyocyte apoptosis in diabetic cardiomyopathy via Neat1/miR-140-5p/HDAC4 axis.
Abstract The cardioprotection of catalpol and its mechanism in diabetic cardiomyopathy (DCM) remains unclear. Here, mouse cardiomyocytes were treated with high glucose (HG) to establish a model of cellular injury induced by HG. In vitro experiments were carried out and confirmed that Catalpol attenuated HG-induced long noncoding RNA (lncRNA) nuclear paraspeckle assembly transcript 1 (Neat1) expression in mouse cardiomyocytes. Mechanistically, luciferase reporter analysis indicated that Neat1 could decrease the transcription of miR-140-5p to positively regulate histone deacetylase 4 (HDAC4) expression. Notably, ove...
Source: Biochimie - May 9, 2019 Category: Biochemistry Authors: Zou G, Zhong W, Wu F, Wang X, Liu L Tags: Biochimie Source Type: research

Tanshinone IIA-mediated inhibition on miR-125b/STARD13 axis attenuates the stemness and enhances adriamycin sensitivity of breast cancer cells.
Abstract The roles of Tanshinone IIA in regulating the stemness of tumor cells remain unclear. This work aims to explore the roles and underlying mechanisms of Tanshinone IIA in breast cancer cell stemness. We found that Tanshinone IIA attenuated the stemness of breast cancer cells, evident by downregulating the expression of stemness markers, hindering the capacity of spheroid formation and decreasing the CD24-/CD44+ sub-population in a concentration-dependent manner. Additionally, Tanshinone IIA enhanced adriamycin sensitivity and attenuated adriamycin resistance of breast cancer cells. Mechanistical studies fou...
Source: Biochimie - May 9, 2019 Category: Biochemistry Authors: Li X, Zheng L, Xi T Tags: Biochimie Source Type: research

Exploiting the unique features of Zika and Dengue proteases for inhibitor design.
aacute; E, Konvalinka J Abstract Zika and Dengue viruses have attracted substantial attention from researchers in light of recent outbreaks of Dengue fever and increases in cases of congenital microcephaly in areas with Zika incidence. This review summarizes the current state of knowledge about Zika and Dengue proteases. These enzymes have several interesting features: 1) NS3 serine protease requires the activating co-factor NS2B, which is anchored in the membrane of the endoplasmic reticulum; 2) NS2B displays extensive conformational dynamics; 3) NS3 is a multidomain protein with proteolytic, NTPase, RNA 5' triph...
Source: Biochimie - May 8, 2019 Category: Biochemistry Authors: Majerová T, Novotný P, Krýsová E, Konvalinka J Tags: Biochimie Source Type: research

Herbacetin suppressed MMP9 mediated angiogenesis of malignant melanoma through blocking EGFR-ERK/AKT signaling pathway.
In this study, the anti-angiogenesis effect of Herbacetin in human malignant melanoma was investigated. The results indicated that Herbacetin treatment significantly suppressed tumor growth and angiogenesis of malignant melanoma both in vitro and in vivo. In melanoma A375 and Hs294T cells, Herbacetin treatment suppressed both EGF-induced and constitutive phosphorylation of EGFR, accelerated the internalization and degradation of EGFR, and subsequently suppressed the activation of the downstream kinases (AKT and ERK). Moreover, MMP9 was determined as a key angiogenic factor in Herbacetin treated melanoma cells. Knockdown of...
Source: Biochimie - May 7, 2019 Category: Biochemistry Authors: Li L, Fan P, Chou H, Li J, Wang K, Li H Tags: Biochimie Source Type: research

Rationally designed small molecules targeting toxic CAG repeat RNA that causes Huntington's disease (HD) and spinocerebellar ataxia (SCAs).
Abstract Huntington's diseases (HD) is a very devastating disease caused by r(CAG) expansion in HTT gene, encoding the huntingtin protein. r(CAG) expansion causes disease via multiple pathways including, 1) loss of normal protein function like sequestration of RNA binding protein such as Muscleblind-like (MBNL) and nucleolin, 2) Gain of function for mutant proteins and 3) repeat-associated non-ATG (RAN) translation; in which expanded r(CAG) translates into toxic poly glu, poly ser, or poly ala without the use of any canonical start codon. Herein, we have rationally designed and synthesized a unique class of pyrido...
Source: Biochimie - May 7, 2019 Category: Biochemistry Authors: Khan E, Biswas S, Mishra SK, Mishra R, Samanta S, Mishra A, Tawani A, Kumar A Tags: Biochimie Source Type: research

A Systems Biology approach to propose a new mechanism of regulation of repetitive prophylaxis of stable Iodide on Sodium/Iodide Symporter (NIS).
Abstract Our group showed that repetitive dose of potassium iodide (KI) for eight days offers an efficient protection for exposure to repeated radioactive emissions without adverse effects on adult rats. However, differential expression of genes implicated in Wolff-Chaikoff effect was observed. To understand the Wolff-Chaikoff regulation and its molecular constituents during repetitive administration of KI, a biochemical reaction network was constructed as a "geographical" map of the thyrocyte depicting iodide and thyroid hormone synthesis. Path analysis of the network has been performed to investigate t...
Source: Biochimie - May 6, 2019 Category: Biochemistry Authors: Cohen DPA, Lebsir D, Benderitter M, Souidi M Tags: Biochimie Source Type: research

Cystatins in cancer progression: More than just cathepsin inhibitors.
Abstract Cystatins are endogenous and reversible inhibitors of cysteine peptidases that are important players in cancer progression. Besides their primary role as regulators of cysteine peptidase activity, cystatins are involved in cancer development and progression through proteolysis-independent mechanisms. Mechanistic studies of cystatin function revealed that they affect all stages of cancer progression including tumor growth, apoptosis, invasion, metastasis and angiogenesis. Recently, the involvement of cystatins in the antitumor immune responses was reported. In this review, we discuss molecular mechanisms a...
Source: Biochimie - May 6, 2019 Category: Biochemistry Authors: Breznik B, Mitrović A, Lah TT, Kos J Tags: Biochimie Source Type: research

Blood anticlotting activity of a Rhipicephalus microplus cathepsin L-like enzyme.
In conclusion, BmCL1 likely has an additional role in R. microplus blood digestion, besides its role in hemoglobin degradation at acid pH. BmCL1 fibrinogenolytic activity indicates a proteolytic activity in the neutral lumen of tick midgut, contributing to maintain the fluidity of the ingested blood, which remains to be confirmed in vivo. PMID: 31059753 [PubMed - as supplied by publisher] (Source: Biochimie)
Source: Biochimie - May 3, 2019 Category: Biochemistry Authors: Xavier MA, Tirloni L, Torquato R, Tanaka A, Pinto AFM, Diedrich JK, Yates JR, da Silva Vaz I, Seixas A, Termignoni C Tags: Biochimie Source Type: research

Flavonoid loaded nanoparticles as an effective measure to combat oxidative stress in Ribonuclease A.
Abstract Flavonoids like quercetin and myricetin serve as naturally occurring antioxidants but their bioactivity is limited due to low aqueous solubility and oxidation under physiological conditions. In this current study, the antioxidant activity of quercetin and myricetin loaded chitosan nanoparticles during the induced oxidation of Ribonuclease A (RNase A) has been compared with the corresponding free flavonoids. Oxidation of RNase A leads to intermolecular dityrosine (DT) bond formation which shows a characteristic fluorescence emission around 405 nm. Although both quercetin and myricetin loaded nanoparticles ...
Source: Biochimie - May 3, 2019 Category: Biochemistry Authors: Roy P, Parveen S, Ghosh P, Ghatak K, Dasgupta S Tags: Biochimie Source Type: research

Palmatine: A Review of its Pharmacology, Toxicity and Pharmacokinetics.
Abstract Palmatine is a natural isoquinoline alkaloid and has been widely used in pharmaceutical field. The purpose of this review is to provide the latest and comprehensive information on the pharmacology, toxicity and pharmacokinetics of palmatine in the past, to explore the therapeutic potential of this compound and look for ways to reduce toxicity. Information on palmatine was collected from the internet database PubMed, Elsevier, ResearchGate, Web of Science, Wiley Online Library and Europe PMC using a combination of keywords including "pharmacology", "toxicology", "pharmacokinetics&q...
Source: Biochimie - April 30, 2019 Category: Biochemistry Authors: Long J, Song J, Zhong L, Liao Y, Liu L, Li X Tags: Biochimie Source Type: research

5,7,3',4'-Hydroxy substituted flavonoids reduce the heme of cytochrome c with a range of rate constants.
Abstract Flavonoids are antioxidants known to be abundant in edible plants. Seven 5,7,3',4'-tetrahydroxy substituted flavonoids representing each major flavonoid class were used as cytochrome c reductants to systematically investigate the redox role of their C-rings. Additional examples of flavonoids and benzenediols were investigated to better understand the role of the B-ring. Pseudo-first order rate constants (kobs) and apparent bimolecular rate constants (kapp) values were calculated from spectroscopic measurements. Of the seven flavonoids tested, five yielded measurable observed reduction rate constants. Bute...
Source: Biochimie - April 26, 2019 Category: Biochemistry Authors: Smith MR, Kindl ED, Williams IR, Moorman VR Tags: Biochimie Source Type: research

A large-scale comparative analysis of affinity, thermodynamics and functional characteristics of twelve cytochrome P450 isoforms and their redox partners.
Abstract The aim of the present work was to establish the thermodynamic and functional differences in the protein-protein interactions between the components of the P450-dependent mitochondrial (mit) and microsomal (mic) monooxygenase systems using 12 different isoforms of cytochromes P450 and two redox partners, NADPH-dependent cytochrome P450 reductase (CPR) and adrenodoxin (Adx). Comparative analysis of the affinity, thermodynamics, enzymatic activity and the ability for one-electron reduction has been carried out. The study of protein-protein interactions to determine the equilibrium dissociation constants (Kd...
Source: Biochimie - April 26, 2019 Category: Biochemistry Authors: Yablokov ЕО, Sushko ТА, Ershov PV, Florinskaya АV, Gnedenko ОV, Shkel ТV, Grabovec IP, Strushkevich NV, Kaluzhskiy LA, Usanov SА, Gilep АА, Ivanov АS Tags: Biochimie Source Type: research

Application of Nanotechnology to target and exploit tumour associated proteases.
Abstract Proteases are hydrolytic enzymes fundamental for a variety of physiological processes, but the loss of their regulation leads to aberrant functions that promote onset and progression of many diseases including cancer. Proteases have been implicated in almost every hallmark of cancer and whilst widely investigated for tumour therapy, clinical adoption of protease drugs remains a challenge due to issues such as off-target toxicity and inability to achieve therapeutic doses at the disease site. Now, nanotechnology-based solutions and strategies are emerging to circumvent these issues. In this review, preclin...
Source: Biochimie - April 25, 2019 Category: Biochemistry Authors: Cogo F, Williams R, Burden RE, Scott CJ Tags: Biochimie Source Type: research

Long non-coding RNA Linc00221 mediates cisplatin resistance in non-small-cell lung cancer (NSCLC) via sponging miR-519a.
In conclusion, Linc00221 promotes cisplatin resistance in NSCLC through the downstream miR-519a/ZBTB5 signaling axis, which could be used as a potential diagnostic and therapeutic target for clinical cisplatin-resistant NSCLC patients. PMID: 31029744 [PubMed - as supplied by publisher] (Source: Biochimie)
Source: Biochimie - April 25, 2019 Category: Biochemistry Authors: Tang H, Han X, Li M, Li T, Hao Y Tags: Biochimie Source Type: research

Benzothiazole derivatives as fluorescent "light-up" probes for duplex and quadruplex DNA.
Benzothiazole derivatives as fluorescent "light-up" probes for duplex and quadruplex DNA. Biochimie. 2019 Apr 22;: Authors: Turaev AV, Tsvetkov VB, Tankevich MV, Smirnov IP, Aralov AV, Pozmogova GE, Varizhuk AM Abstract Analogs of benzothiazole orange (BO) with one, two or three methylbenzothiazolylmethylidene substituents in the 1-methylpyridinium ring were obtained from the respective picolinium, lutidinium or collidinium salts. Fluorescence parameters of the known and new dyes in complexes with various DNA structures, including G-quadruplexes (G4s) and i-motifs (IMs), were analyzed. All d...
Source: Biochimie - April 22, 2019 Category: Biochemistry Authors: Turaev AV, Tsvetkov VB, Tankevich MV, Smirnov IP, Aralov AV, Pozmogova GE, Varizhuk AM Tags: Biochimie Source Type: research

TLR2 and TLR4 mediate an activation of adipose tissue renin-angiotensin system induced by uric acid.
In conclusion, TLR2/4 mediated adipose inflammation plays a key role in RAS activation induced by uric acid in adipose tissue. PMID: 31002842 [PubMed - as supplied by publisher] (Source: Biochimie)
Source: Biochimie - April 16, 2019 Category: Biochemistry Authors: Zhang J, Diao B, Xue Lin, Xu J, Tang F Tags: Biochimie Source Type: research

Metabolic and physiological perturbations of Escherichia coli W3100 by bacterial small RNA RyhB.
Abstract RyhB is a key regulator of iron level in Escherichia coli (E. coli), which assists in conserving iron for life-sustaining cellular functions when cytoplasmic levels of the ferrous form of iron is limited. RyhB affects glucose metabolism. Seventy percent of the genes that are regulated by RyhB are related to metabolism. We demonstrated for the first time that the activity of the pentose phosphate pathway increased upon ryhB activation using a 13C stable isotope-based technique called METAFoR (Metabolic flux ratio analysis). U-13C glucose-based studies showed that the reversible exchange activity of serine ...
Source: Biochimie - April 16, 2019 Category: Biochemistry Authors: Lv Y, Wu J, Shi Y Tags: Biochimie Source Type: research

Up-regulation of microRNA-496 suppresses proliferation, invasion, migration and in vivo tumorigenicity of human osteosarcoma cells by targeting eIF4E.
This study aims to investigate how microRNA-496 (miR-496) affects proliferation, invasion, and migration of human osteosarcoma (OS) cells and in vivo tumorigenicity by targeting eukaryotic translation initiation factor 4E (eIF4E). Microarray-based gene expression profiling involving OS was used in order to identify differentially expressed genes. After that, the interaction between miR-496 expression and OS patients' survival rate was determined. The expression pattern of miR-496 and eIF4E was determined in OS tissues and cells, and their potential relationship was further analyzed by using the dual luciferase reporter gen...
Source: Biochimie - April 15, 2019 Category: Biochemistry Authors: Qi NN, Tian S, Li X, Wang FL, Liu B Tags: Biochimie Source Type: research

Physiological roles of antisense RNAs in prokaryotes.
Abstract Prokaryotes encounter constant and often brutal modifications to their environment. In order to survive, they need to maintain fitness, which includes adapting their protein expression patterns. Many factors control gene expression but this review focuses on just one, namely antisense RNAs (asRNAs), a class of non-coding RNAs (ncRNAs) characterized by their location in cis and their perfect complementarity with their targets. asRNAs were considered for a long time to be trivial and only to be found on mobile genetic elements. However, recent advances in methodology have revealed that their abundance and p...
Source: Biochimie - April 14, 2019 Category: Biochemistry Authors: Lejars M, Kobayashi A, Hajnsdorf E Tags: Biochimie Source Type: research

CREPT promotes glioma cell proliferation and invasion by activating Wnt/ β-catenin pathway and is a novel target of microRNA-596.
CREPT promotes glioma cell proliferation and invasion by activating Wnt/β-catenin pathway and is a novel target of microRNA-596. Biochimie. 2019 Apr 14;: Authors: Wei M, Cao Y, Jia D, Zhao H, Zhang L Abstract Cell cycle-related and expression elevated protein in tumor (CREPT) is emerging as a novel cancer-related gene that is dysregulated in many kinds of malignancies. However, the expression and biological role of CREPT in glioma remains unclear. In the present study, we aimed to explore the potential function and regulation mechanism of CREPT in glioma. Results showed that CRETP expression was ...
Source: Biochimie - April 14, 2019 Category: Biochemistry Authors: Wei M, Cao Y, Jia D, Zhao H, Zhang L Tags: Biochimie Source Type: research

"70 years of serotonin".
"70 years of serotonin". Biochimie. 2019 Apr 12;: Authors: Côté F, Vaillancourt C PMID: 30986434 [PubMed - as supplied by publisher] (Source: Biochimie)
Source: Biochimie - April 12, 2019 Category: Biochemistry Authors: Côté F, Vaillancourt C Tags: Biochimie Source Type: research

Crataeva tapia bark lectin (CrataBL) is a chemoattractant for endothelial cells that targets heparan sulfate and promotes in vitro angiogenesis.
e Paula CA, Vilela Oliva ML Abstract Formation of new blood vessels from preexisting ones, a process known as angiogenesis, is one of the limiting steps for success in treatment of ischemic disorders. Therefore, efforts to understanding and characterize new agents capable to stimulate neovascularization are a worldwide need. Crataeva tapia bark lectin (CrataBL) has been shown to have chemoattractant properties for endothelial cells through the stimulation of migration and invasiveness of human umbilical vein endothelial cells (HUVEC) because it is a positively charged protein with high affinity to glycosaminoglyca...
Source: Biochimie - April 11, 2019 Category: Biochemistry Authors: Batista FP, Barbosa de Aguiar R, Sumikawa JT, Lobo YA, Bonturi CR, Ferreira da Silva R, Andrade SS, Guedes Paiva PM, Tereza Dos Santos Correia M, Vicente CM, Toma L, Sampaio MU, Paschoalin T, Batista Castello Girão MJ, Zveiter de Moraes J, Andrade de Pau Tags: Biochimie Source Type: research

GLUT1 is associated with sphingolipid-organized, cholesterol-independent domains in L929 mouse fibroblast cells.
Abstract Glucose is a preferred metabolite in most mammalian cells, and proper regulation of uptake is critical for organism homeostasis. The glucose transporter 1 (GLUT1) is responsible for glucose uptake in a wide variety of cells and appears to be regulated in a tissue specific manner. Therefore, a better understanding of GLUT1 regulation within its various cellular environments is essential for developing therapeutic strategies to treat disorders associated with glucose homeostasis. Previous findings suggest that plasma membrane subdomains called lipid rafts may play a role in regulation of GLUT1 uptake activi...
Source: Biochimie - April 10, 2019 Category: Biochemistry Authors: Rylaarsdam LE, Johnecheck GN, Looyenga BD, Louters LL Tags: Biochimie Source Type: research

In vivo assembly of eukaryotic signal recognition particle: a still enigmatic process involving the SMN complex.
Abstract The signal recognition particle (SRP) is a universally conserved non-coding ribonucleoprotein complex that is essential for targeting transmembrane and secretory proteins to the endoplasmic reticulum. Its composition and size varied during evolution. In mammals, SRP contains one RNA molecule, 7SL RNA, and six proteins: SRP9, 14, 19, 54, 68 and 72. Despite a very good understanding of the SRP structure and of the SRP assembly in vitro, how SRP is assembled in vivo remains largely enigmatic. Here we review current knowledge on how the 7SL RNA is assembled with core proteins to form functional RNP particles ...
Source: Biochimie - April 9, 2019 Category: Biochemistry Authors: Massenet S Tags: Biochimie Source Type: research

Identification of a peptide derived from a Bothrops moojeni metalloprotease with in vitro inhibitory action on the Plasmodium falciparum purine nucleoside phosphorylase enzyme (PfPNP).
es AM Abstract There is a growing need for research on new antimalarial agents against Plasmodium falciparum infection, especially in regards to planning molecular architecture for specific molecular targets of the parasite. Thus, a metalloprotease from Bothrops moojeni, known as BmooMPα-I, was explored in this study, through in silico assays, aiming at the development of a peptide generated from this molecule with potential inhibitory action on PfPNP, an enzyme necessary for the survival of the parasite. In order to isolate BmooMPα-I, cation exchange and reverse phase chromatographies were performed, ...
Source: Biochimie - April 9, 2019 Category: Biochemistry Authors: Martins GG, de Jesus Holanda R, Alfonso J, Gómez Garay AF, Santos APAD, Maciel de Lima A, Francisco AF, Garcia Teles CB, Zanchi FB, Soares AM Tags: Biochimie Source Type: research

Kinetics of inactivation of staphylolytic enzymes: qualitative and quantitative description.
Abstract Lysin 2638aR and chimeric Ply187AN-KSH3b fusion protein are capable of lysing antibiotic-resistant strains of Staphylococcus aureus and are promising alternatives to antibiotics. Studies on the stability and structure of lysins 2638aR and Ply187AN-KSH3b are important for assessing the feasibility of their practical use. Both lysins are highly active at physiological pH (7.5) and at low salt content (the concentration of NaCl in the reaction medium is not more than 250 mM). Lysins are inactivated by a monomolecular mechanism and have high stability at 4°C (storage temperature). The maximum value of the...
Source: Biochimie - April 6, 2019 Category: Biochemistry Authors: Filatova L, Donovan D, Swift S, Pugachev V, Emelianov G, Chubar T, Klaychko N Tags: Biochimie Source Type: research

Suppression in advanced glycation adducts of human serum albumin by bio-enzymatically synthesized gold and silver nanoformulations: A potential tool to counteract hyperglycemic condition.
Abstract Advanced glycation end-products (AGEs) from non-enzymatic glycation are implicated in several degenerative diseases, including being a crucial contributor in secondary complications of diabetes. This has garnered significant scientific interest in inhibiting agents of AGEs to prevent and remediate disorders arising due to glycation. In the current study, inhibitory effects on AGEs formation were investigated using bio-enzymatically synthesized nanoformulations of gold (AuNPs) and silver (AgNPs) by physiologically important enzyme, β galactosidase. Human serum albumin, most abundant protein in human b...
Source: Biochimie - April 5, 2019 Category: Biochemistry Authors: Ahmed F, Husain Q Tags: Biochimie Source Type: research

The homeoprotein Msx1 cooperates with Pkn1 to prevent terminal differentiation in myogenic precursor cells.
Abstract The homeoprotein Msx1 plays a critical role in embryonic patterning, particularly to maintain myogenic progenitor cell fate. However, the mechanisms underlying Msx1-mediated inhibition of myogenesis remain largely unknown. Here, we show that Msx1 cooperates with the protein kinase C-related kinase 1 (Pkn1), a member of the protein kinase C-related kinase family, to prevent the terminal differentiation of myogenic precursor cells. In mouse C2C12 cells, Pkn1 knockout partly impaired Msx1-mediated inhibition of myogenic differentiation, indicating a role for Pkn1 in this process. Furthermore, we found that P...
Source: Biochimie - April 5, 2019 Category: Biochemistry Authors: Zhu X, Li M, Jia X, Hou W, Yang J, Zhao H, Wang G, Wang J Tags: Biochimie Source Type: research

The apoptosis and GLP-1 hyposecretion induced by LPS via RIP/ROS/mTOR pathway in GLUTag cells.
Abstract Lipopolysaccharide (LPS) as a component of the outer structure of cell wall of gram-negative bacteria, could induce apoptosis in the intestinal endocrine cell line STC-1. However, the signaling cascades involved in this process have not been elucidated. Hence, we investigated the mechanism of cell apoptosis and hyposecretion of glucagon-like peptide 1 (GLP-1) induced by LPS in the GLUTag enteroendocrine cell line. LPS decreased the cell viability of GLUTag cells, up-regulated the TNF-α level, induced the apoptosis and down-regulated the mRNA and protein levels of GLP-1. In addition, TNF-α prom...
Source: Biochimie - April 4, 2019 Category: Biochemistry Authors: Wang J, Wang X, Li ZZ, Guo F, Ding CZ, Zhao YY, Liu YL, Ma XJ, Li C, Wu LN, Qin Q, Zhao SY, Zhao D, Hao X, Wang SJ, Qin GJ Tags: Biochimie Source Type: research

Serotonin and orthodontic tooth movement.
F, Coman T Abstract Peripheral serotonin continuously reveals its unexpected involvements in many organ functions. In bone tissue, there is an increasing evidence for a local serotonergic system affecting the cellular and molecular actors involved in bone turnover. During orthodontic treatment, tooth movement relies on bone remodeling, itself a result of the inflammatory process triggered by application of orthodontic forces to the teeth. Nowadays, many adults proceed to an orthodontic treatment, it therefore seems important to consider physiological growth-related factors and external factors as medications that ...
Source: Biochimie - April 3, 2019 Category: Biochemistry Authors: Dhenain T, Côté F, Coman T Tags: Biochimie Source Type: research

An ancient mechanism of arginine-specific substrate cleavage: what's up with NSP4?
Abstract The recently discovered neutrophil serine protease 4 (NSP4) is the fourth member of the NSP family, which includes the well-studied neutrophil elastase, proteinase 3 and cathepsin G. Like the other three NSP members, NSP4 is synthesized by myeloid precursors in the bone marrow and, after cleavage of the two-amino acid activation peptide, is stored as an active protease in azurophil granules of neutrophils. Based on its primary amino acid sequence, NSP4 is predicted to have a shallow S1 specificity pocket with elastase-like substrate specificity. However, NSP4 was found to preferentially cleave after an ar...
Source: Biochimie - April 1, 2019 Category: Biochemistry Authors: AhYoung AP, Lin SJ, Gerhardy S, van Lookeren Campagne M, Kirchhofer D Tags: Biochimie Source Type: research

Biochemical and molecular characterization of the hyaluronidase from Bothrops atrox Peruvian snake venom.
In this study, we describe the biochemical and molecular features of a hyaluronidase (Hyal-Ba) isolated from the venom of the Peruvian snake Bothrops atrox. Hyal-Ba was purified by a combination of ion-exchange and gel filtration chromatography. Purified Hyal-Ba is a 69-kDa (SDS-PAGE) monomeric glycoprotein with an N-terminal amino acid sequence sharing high identity with homologous snake venom hyaluronidases. Detected associated carbohydrates were hexoses (16.38%), hexosamines (2.7%) and sialic acid (0.69%). Hyal-Ba selectively hydrolyzed only hyaluronic acid (HA; specific activity = 437.5 U/mg) but it did not hydrolyze c...
Source: Biochimie - April 1, 2019 Category: Biochemistry Authors: Vivas-Ruiz DE, Gonzalez-Kozlova EE, Delgadillo J, Palermo PM, Sandoval GA, Lazo F, Rodríguez E, Chávez-Olórtegui C, Yarlequé A, Sanchez EF Tags: Biochimie Source Type: research

Repurposing of auranofin: thioredoxin reductase remains a primary target of the drug.
der S Abstract Auranofin is a gold (I)-containing compound used for the treatment of rheumatic arthritis. Auranofin has anticancer activity in animal models and is approved for clinical trials for lung and ovarian carcinomas. Both the cytosolic and mitochondrial forms of the selenoprotein thioredoxin reductase (TrxR) are well documented targets of auranofin. Auranofin was recently reported to inhibit proteasome activity at the level of the proteasome-associated deubiquitinases (DUBs) UCHL5 and USP14. We here set out to re-examine the molecular mechanism underlying auranofin cytotoxicity towards cultured cancer cel...
Source: Biochimie - April 1, 2019 Category: Biochemistry Authors: Zhang X, Selvaraju K, Saei AA, D'Arcy P, Zubarev RA, Arnér ES, Linder S Tags: Biochimie Source Type: research

Serotonin-estrogen interactions: what can we learn from pregnancy?
Abstract We have reviewed the scientific literature related to four diseases in which to serotonin (5-HT) is involved in the etiology, herein named 5-HT-linked diseases, and whose prevalence is influenced by estrogenic status: depression, migraine, irritable bowel syndrome and eating disorders. These diseases all have in common a sex-dimorphic prevalence, with women more frequently affected than men. The co-occurrence between these 5-HT-linked diseases suggests that they have common physiopathological mechanisms. In most 5-HT-linked diseases (except for anorexia nervosa and irritable bowel syndrome), a decrease in...
Source: Biochimie - April 1, 2019 Category: Biochemistry Authors: Hudon Thibeault AA, Sanderson JT, Vaillancourt C Tags: Biochimie Source Type: research

Design of cross-linked RNA/protein complexes for structural studies.
We present here a method to trap the two interacting partners in a covalent complex, based on a modified reactive RNA allowing the use of the full range of common crystallogenesis tools. We demonstrate the practicability of our approach with the production of a covalent complex of the Thermus thermophilus m1A58 tRNA modification enzyme, and a modified stem loop mimicking the natural substrate of the enzyme. PMID: 30940603 [PubMed - as supplied by publisher] (Source: Biochimie)
Source: Biochimie - March 30, 2019 Category: Biochemistry Authors: Dégut C, Schwarz V, Ponchon L, Barraud P, Micura R, Tisné C Tags: Biochimie Source Type: research

Sex-specific polymorphism of MET1 and ARR17 genes in Populus × sibirica.
Sex-specific polymorphism of MET1 and ARR17 genes in Populus × sibirica. Biochimie. 2019 Mar 29;: Authors: Melnikova NV, Kudryavtseva AV, Borkhert EV, Pushkova EN, Fedorova MS, Snezhkina AV, Krasnov GS, Dmitriev AA Abstract The genus Populus is an effective model in tree genetics. This genus includes dioecious species and, recently, whole genome resequencing of P. trichocarpa and P. balsamifera enabled the identification of sex-linked regions and sex-associated single nucleotide polymorphisms (SNPs). These results created new opportunities to study sex determination in poplars. In the present wo...
Source: Biochimie - March 29, 2019 Category: Biochemistry Authors: Melnikova NV, Kudryavtseva AV, Borkhert EV, Pushkova EN, Fedorova MS, Snezhkina AV, Krasnov GS, Dmitriev AA Tags: Biochimie Source Type: research

SOX11 hypermethylation as a tumor biomarker in endometrial cancer.
PMID: 30935961 [PubMed - as supplied by publisher] (Source: Biochimie)
Source: Biochimie - March 29, 2019 Category: Biochemistry Authors: Shan T, Uyar DS, Wang LS, Mutch DG, Huang TH, Rader JS, Sheng X, Huang YW Tags: Biochimie Source Type: research

Dual cellular localization of the Leishmania amazonensis Rbp38 (LaRbp38) explains its affinity for telomeric and mitochondrial DNA.
Abstract Rbp38 is a protein exclusively found in trypanosomatid parasites, including Leishmania amazonensis, the etiologic agent of tegumentar leishmaniasis in the Americas. The protein was first described as a Leishmania tarentolae mitochondrial RNA binding protein. Later, it was shown that the trypanosomes Rbp38 orthologues were exclusively found in the mitochondria and involved in the stabilization and replication of kinetoplast DNA (kDNA). In contrast, L. amazonensis Rbp38 (LaRbp38), co-purifies with telomerase activity and interacts not only with kDNA but also with telomeric DNA, although shares with its coun...
Source: Biochimie - March 28, 2019 Category: Biochemistry Authors: Fernandes CAH, Perez AM, Barros AC, Dreyer TR, da Silva MS, Morea EGO, Fontes MRM, Cano MIN Tags: Biochimie Source Type: research

Structural insights into the 3'-end mRNA maturation machinery: snapshot on Polyadenylation signal recognition.
Abstract Pre-mRNA 3'-end maturation is achieved by a mechanism requiring four different protein complexes assembled from approximately twenty factors. A global understanding of this essential process is still missing due to the inability to structurally characterize the entire complexes, even though structures of the isolated factors have been obtained. In this review, we summarize recent findings regarding the atomic description of one the major players, the Cleavage and Polyadenylation Specificity Factor complex (CPSF in human, CPF in yeast). These data provide information on the architecture adopted by the majo...
Source: Biochimie - March 28, 2019 Category: Biochemistry Authors: Thore S, Fribourg S Tags: Biochimie Source Type: research

Differential micro-RNA expression in diabetic patients with abdominal aortic aneurysm.
ort J Abstract OBJECTIVES: The potential implication of micro-RNAs (miRs) in the negative association between diabetes and abdominal aortic aneurysm (AAA) has so far never been addressed. The aim of this study was to compare miR expression between diabetic and non-diabetic patients with AAA. METHODS: Ten diabetic patients were prospectively included and compared to 10 age- and sex-matched non-diabetic patients with infrarenal AAA. A profiling analysis of 752 human miRs was performed from peripheral blood mononuclear cells (PBMCs) using miRCURY LNA Universal RT microRNA PCR (Exiqon- Qiagen®). miR that show...
Source: Biochimie - March 25, 2019 Category: Biochemistry Authors: Lareyre F, Clément M, Moratal C, Loyer X, Jean-Baptiste E, Hassen-Khodja R, Chinetti G, Mallat Z, Raffort J Tags: Biochimie Source Type: research

The yeast C/D box snoRNA U14 adopts a "weak" K-turn like conformation recognized by the Snu13 core protein in solution.
The yeast C/D box snoRNA U14 adopts a "weak" K-turn like conformation recognized by the Snu13 core protein in solution. Biochimie. 2019 Mar 23;: Authors: Chagot ME, Quinternet M, Rothé B, Charpentier B, Coutant J, Manival X, Lebars I Abstract Non-coding RNAs associate with proteins to form ribonucleoproteins (RNPs), such as ribosome, box C/D snoRNPs, H/ACA snoRNPs, ribonuclease P, telomerase and spliceosome to ensure cell viability. The assembly of these RNA-protein complexes relies on the ability of the RNA to adopt the correct bound conformation. K-turn motifs represent ubiquitous b...
Source: Biochimie - March 23, 2019 Category: Biochemistry Authors: Chagot ME, Quinternet M, Rothé B, Charpentier B, Coutant J, Manival X, Lebars I Tags: Biochimie Source Type: research

Imaging of extracellular cathepsin S activity by a selective near infrared fluorescence substrate-based probe.
Abstract We designed a near-infrared fluorescent substrate-based probe (SBP), termed MG101, for monitoring extracellular cathepsin S (CatS) activity. We conceived a fused peptide hairpin loop-structure, combining a CatS recognition domain, an electrostatic zipper (with complementary charges of a polyanionic (D-Glu)5 segment and a polycationic (D-Arg)5 motif, as well as a N- and C- terminal Förster resonance energy transfer pair (donor: AlexaFluor680; quencher: BHQ3) to facilitate activity-dependent imaging. MG101 showed excellent stability since no fluorescence release corresponding to a self-dequenching was ...
Source: Biochimie - March 23, 2019 Category: Biochemistry Authors: Wartenberg M, Saidi A, Galibert M, Joulin-Giet A, Burlaud-Gaillard J, Lecaille F, Scott CJ, Aucagne V, Delmas AF, Lalmanach G Tags: Biochimie Source Type: research

HIV-1 gRNA, a biological substrate, uncovers the potency of DDX3X biochemical activity.
In this study, we investigated DDX3 biochemical properties in the context of a biological substrate. DDX3 was overexpressed, purified and its enzymatic activities as well as its RNA binding properties were characterized using both model substrates and a biological substrate, HIV-1 gRNA. Biochemical characterization of DDX3 in the context of a biological substrate identifies HIV-1 gRNA as a rare example of specific substrate and unravels the extent of DDX3 ATPase activity. Analysis of DDX3 binding capacity indicates an unexpected dissociation between its binding capacity and its biochemical activity. We further demonstrate ...
Source: Biochimie - March 22, 2019 Category: Biochemistry Authors: de Bisschop G, Ameur M, Ulryck N, Benattia F, Ponchon L, Sargueil B, Chamond N Tags: Biochimie Source Type: research

MicroRNA-34 suppresses proliferation of human ovarian cancer cells by triggering autophagy and apoptosis and inhibits cell invasion by targeting Notch 1.
This study was designed to investigate the role and therapeutic potential of miR-34 in ovarian cancer. It was found that miR-34 is significantly downregulated in ovarian cancer cell lines. Overexpression of miR-34 causes significant decrease in the proliferation of OVACAR-3 ovarian cancer cells via activation of apoptosis and autophagy. The miR-34 overexpression was concomitant with upsurge of apoptosis related proteins (Bax) and the autophagy associated protein (LC3 II and p62). TargetScan analysis showed Notch 1 to be the main target of miR-34 in OVACAR-3 cells which was further validated by luciferase reporter assay. Th...
Source: Biochimie - March 21, 2019 Category: Biochemistry Authors: Jia Y, Lin R, Jin H, Si L, Jian W, Yu Q, Yang S Tags: Biochimie Source Type: research

Heavy metal hypertolerant eukaryotic aldehyde dehydrogenase isolated from metal contaminated soil by metatranscriptomics approach.
This study proves that PLCc38 is a potent gene associated with metal tolerance which could be used to revegetate heavy metal polluted soil or as a biomarker for detection of metal contamination. PMID: 30905733 [PubMed - as supplied by publisher] (Source: Biochimie)
Source: Biochimie - March 21, 2019 Category: Biochemistry Authors: Mukherjee A, Yadav R, Marmeisse R, Fraissinet-Tachet L, Reddy MS Tags: Biochimie Source Type: research

Highly active extracellular α-class carbonic anhydrase of Cyanothece sp. ATCC 51142.
Highly active extracellular α-class carbonic anhydrase of Cyanothece sp. ATCC 51142. Biochimie. 2019 Mar 18;: Authors: Kupriyanova EV, Sinetova MA, Mironov KS, Novikova GV, Dykman LA, Rodionova MV, Gabrielyan DA, Los DA Abstract Here, for the first time, we report the presence of highly active extracellular carbonic anhydrase (CA) of α-class in cyanobacterial cells. The enzyme activity was confirmed both in vivo in intact cells and in vitro, using the recombinant protein. CA activity in intact cells of Cyanothece sp. ATCC 51142 reached ∼ 0.6 Wilbur-Anderson units (WAU) per 1 mg of tota...
Source: Biochimie - March 18, 2019 Category: Biochemistry Authors: Kupriyanova EV, Sinetova MA, Mironov KS, Novikova GV, Dykman LA, Rodionova MV, Gabrielyan DA, Los DA Tags: Biochimie Source Type: research