Alteration of mitochondrial function in the livers of mice with glycogen branching enzyme deficiency
Biochimie. 2021 Apr 12:S0300-9084(21)00098-5. doi: 10.1016/j.biochi.2021.04.001. Online ahead of print.ABSTRACTGlycogen storage disease type IV (GSD IV) is caused by mutations in the glycogen branching enzyme gene (GBE1) that lead to the accumulation of aberrant glycogen in affected tissues, mostly in the liver. To determine whether dysfunctional glycogen metabolism in GSD IV affects other components of cellular bioenergetics, we studied mitochondrial function in heterozygous Gbe1 knockout (Gbe1+/-) mice. Mitochondria isolated from the livers of Gbe1+/- mice showed elevated respiratory complex I activity and increased reac...
Source: Biochimie - April 15, 2021 Category: Biochemistry Authors: Dominika Malinska Giorgia Testoni Malgorzata Bejtka Jordi Duran Joan J Guinovart Jerzy Duszynski Source Type: research

Alteration of mitochondrial function in the livers of mice with glycogen branching enzyme deficiency
Biochimie. 2021 Apr 12:S0300-9084(21)00098-5. doi: 10.1016/j.biochi.2021.04.001. Online ahead of print.ABSTRACTGlycogen storage disease type IV (GSD IV) is caused by mutations in the glycogen branching enzyme gene (GBE1) that lead to the accumulation of aberrant glycogen in affected tissues, mostly in the liver. To determine whether dysfunctional glycogen metabolism in GSD IV affects other components of cellular bioenergetics, we studied mitochondrial function in heterozygous Gbe1 knockout (Gbe1+/-) mice. Mitochondria isolated from the livers of Gbe1+/- mice showed elevated respiratory complex I activity and increased reac...
Source: Biochimie - April 15, 2021 Category: Biochemistry Authors: Dominika Malinska Giorgia Testoni Malgorzata Bejtka Jordi Duran Joan J Guinovart Jerzy Duszynski Source Type: research

Analyses of viral genomes for G-quadruplex forming sequences reveal their correlation with the type of infection
Biochimie. 2021 Apr 8:S0300-9084(21)00096-1. doi: 10.1016/j.biochi.2021.03.017. Online ahead of print.ABSTRACTG-quadruplexes contribute to the regulation of key molecular processes. Their utilization for antiviral therapy is an emerging field of contemporary research. Here we present comprehensive analyses of the presence and localization of putative G-quadruplex forming sequences (PQS) in all viral genomes currently available in the NCBI database (including subviral agents). The G4Hunter algorithm was applied to a pool of 11,000 accessible viral genomes representing 350 Mbp in total. PQS frequencies differ across evolutio...
Source: Biochimie - April 11, 2021 Category: Biochemistry Authors: Nat ália Bohálová Alessio Cantara Martin Bartas Patrik Kaura Ji ří Šťastný Petr Pe činka Miroslav Fojta Jean-Louis Mergny V áclav Brázda Source Type: research

Analyses of viral genomes for G-quadruplex forming sequences reveal their correlation with the type of infection
Biochimie. 2021 Apr 8:S0300-9084(21)00096-1. doi: 10.1016/j.biochi.2021.03.017. Online ahead of print.ABSTRACTG-quadruplexes contribute to the regulation of key molecular processes. Their utilization for antiviral therapy is an emerging field of contemporary research. Here we present comprehensive analyses of the presence and localization of putative G-quadruplex forming sequences (PQS) in all viral genomes currently available in the NCBI database (including subviral agents). The G4Hunter algorithm was applied to a pool of 11,000 accessible viral genomes representing 350 Mbp in total. PQS frequencies differ across evolutio...
Source: Biochimie - April 11, 2021 Category: Biochemistry Authors: Nat ália Bohálová Alessio Cantara Martin Bartas Patrik Kaura Ji ří Šťastný Petr Pe činka Miroslav Fojta Jean-Louis Mergny V áclav Brázda Source Type: research

Analyses of viral genomes for G-quadruplex forming sequences reveal their correlation with the type of infection
Biochimie. 2021 Apr 8:S0300-9084(21)00096-1. doi: 10.1016/j.biochi.2021.03.017. Online ahead of print.ABSTRACTG-quadruplexes contribute to the regulation of key molecular processes. Their utilization for antiviral therapy is an emerging field of contemporary research. Here we present comprehensive analyses of the presence and localization of putative G-quadruplex forming sequences (PQS) in all viral genomes currently available in the NCBI database (including subviral agents). The G4Hunter algorithm was applied to a pool of 11,000 accessible viral genomes representing 350 Mbp in total. PQS frequencies differ across evolutio...
Source: Biochimie - April 11, 2021 Category: Biochemistry Authors: Nat ália Bohálová Alessio Cantara Martin Bartas Patrik Kaura Ji ří Šťastný Petr Pe činka Miroslav Fojta Jean-Louis Mergny V áclav Brázda Source Type: research

Analyses of viral genomes for G-quadruplex forming sequences reveal their correlation with the type of infection
Biochimie. 2021 Apr 8:S0300-9084(21)00096-1. doi: 10.1016/j.biochi.2021.03.017. Online ahead of print.ABSTRACTG-quadruplexes contribute to the regulation of key molecular processes. Their utilization for antiviral therapy is an emerging field of contemporary research. Here we present comprehensive analyses of the presence and localization of putative G-quadruplex forming sequences (PQS) in all viral genomes currently available in the NCBI database (including subviral agents). The G4Hunter algorithm was applied to a pool of 11,000 accessible viral genomes representing 350 Mbp in total. PQS frequencies differ across evolutio...
Source: Biochimie - April 11, 2021 Category: Biochemistry Authors: Nat ália Bohálová Alessio Cantara Martin Bartas Patrik Kaura Ji ří Šťastný Petr Pe činka Miroslav Fojta Jean-Louis Mergny V áclav Brázda Source Type: research

Aquaporin gene transfer for hepatocellular cholestasis
Biochimie. 2021 Mar 31:S0300-9084(21)00086-9. doi: 10.1016/j.biochi.2021.03.016. Online ahead of print.ABSTRACTBile secretion by hepatocytes is an osmotic process. The output of bile salts and other organic anions (e.g. glutathione), through the bile salt transporter BSEP/ABCB11 and the organic anion transporter MRP2/ABCC2, respectively, are considered to be the major osmotic driving forces for water secretion into bile canaliculi mainly via aquaporin-8 (AQP8) channels. The down-regulated canalicular expression of these key solute transporters and AQP8 would be a primary event in the establishment of hepatocellular cholest...
Source: Biochimie - April 3, 2021 Category: Biochemistry Authors: Julieta Marrone Mauro Danielli C ésarI Gaspari Alejo M Capiglioni Ra úl A Marinelli Source Type: research

Aquaporin gene transfer for hepatocellular cholestasis
Biochimie. 2021 Mar 31:S0300-9084(21)00086-9. doi: 10.1016/j.biochi.2021.03.016. Online ahead of print.ABSTRACTBile secretion by hepatocytes is an osmotic process. The output of bile salts and other organic anions (e.g. glutathione), through the bile salt transporter BSEP/ABCB11 and the organic anion transporter MRP2/ABCC2, respectively, are considered to be the major osmotic driving forces for water secretion into bile canaliculi mainly via aquaporin-8 (AQP8) channels. The down-regulated canalicular expression of these key solute transporters and AQP8 would be a primary event in the establishment of hepatocellular cholest...
Source: Biochimie - April 3, 2021 Category: Biochemistry Authors: Julieta Marrone Mauro Danielli C ésarI Gaspari Alejo M Capiglioni Ra úl A Marinelli Source Type: research

Aquaporin gene transfer for hepatocellular cholestasis
Biochimie. 2021 Mar 31:S0300-9084(21)00086-9. doi: 10.1016/j.biochi.2021.03.016. Online ahead of print.ABSTRACTBile secretion by hepatocytes is an osmotic process. The output of bile salts and other organic anions (e.g. glutathione), through the bile salt transporter BSEP/ABCB11 and the organic anion transporter MRP2/ABCC2, respectively, are considered to be the major osmotic driving forces for water secretion into bile canaliculi mainly via aquaporin-8 (AQP8) channels. The down-regulated canalicular expression of these key solute transporters and AQP8 would be a primary event in the establishment of hepatocellular cholest...
Source: Biochimie - April 3, 2021 Category: Biochemistry Authors: Julieta Marrone Mauro Danielli C ésarI Gaspari Alejo M Capiglioni Ra úl A Marinelli Source Type: research

Aquaporin gene transfer for hepatocellular cholestasis
Biochimie. 2021 Mar 31:S0300-9084(21)00086-9. doi: 10.1016/j.biochi.2021.03.016. Online ahead of print.ABSTRACTBile secretion by hepatocytes is an osmotic process. The output of bile salts and other organic anions (e.g. glutathione), through the bile salt transporter BSEP/ABCB11 and the organic anion transporter MRP2/ABCC2, respectively, are considered to be the major osmotic driving forces for water secretion into bile canaliculi mainly via aquaporin-8 (AQP8) channels. The down-regulated canalicular expression of these key solute transporters and AQP8 would be a primary event in the establishment of hepatocellular cholest...
Source: Biochimie - April 3, 2021 Category: Biochemistry Authors: Julieta Marrone Mauro Danielli C ésarI Gaspari Alejo M Capiglioni Ra úl A Marinelli Source Type: research

Aquaporin gene transfer for hepatocellular cholestasis
Biochimie. 2021 Mar 31:S0300-9084(21)00086-9. doi: 10.1016/j.biochi.2021.03.016. Online ahead of print.ABSTRACTBile secretion by hepatocytes is an osmotic process. The output of bile salts and other organic anions (e.g. glutathione), through the bile salt transporter BSEP/ABCB11 and the organic anion transporter MRP2/ABCC2, respectively, are considered to be the major osmotic driving forces for water secretion into bile canaliculi mainly via aquaporin-8 (AQP8) channels. The down-regulated canalicular expression of these key solute transporters and AQP8 would be a primary event in the establishment of hepatocellular cholest...
Source: Biochimie - April 3, 2021 Category: Biochemistry Authors: Julieta Marrone Mauro Danielli C ésarI Gaspari Alejo M Capiglioni Ra úl A Marinelli Source Type: research

Aquaporin gene transfer for hepatocellular cholestasis
Biochimie. 2021 Mar 31:S0300-9084(21)00086-9. doi: 10.1016/j.biochi.2021.03.016. Online ahead of print.ABSTRACTBile secretion by hepatocytes is an osmotic process. The output of bile salts and other organic anions (e.g. glutathione), through the bile salt transporter BSEP/ABCB11 and the organic anion transporter MRP2/ABCC2, respectively, are considered to be the major osmotic driving forces for water secretion into bile canaliculi mainly via aquaporin-8 (AQP8) channels. The down-regulated canalicular expression of these key solute transporters and AQP8 would be a primary event in the establishment of hepatocellular cholest...
Source: Biochimie - April 3, 2021 Category: Biochemistry Authors: Julieta Marrone Mauro Danielli C ésarI Gaspari Alejo M Capiglioni Ra úl A Marinelli Source Type: research

Aquaporin gene transfer for hepatocellular cholestasis
Biochimie. 2021 Mar 31:S0300-9084(21)00086-9. doi: 10.1016/j.biochi.2021.03.016. Online ahead of print.ABSTRACTBile secretion by hepatocytes is an osmotic process. The output of bile salts and other organic anions (e.g. glutathione), through the bile salt transporter BSEP/ABCB11 and the organic anion transporter MRP2/ABCC2, respectively, are considered to be the major osmotic driving forces for water secretion into bile canaliculi mainly via aquaporin-8 (AQP8) channels. The down-regulated canalicular expression of these key solute transporters and AQP8 would be a primary event in the establishment of hepatocellular cholest...
Source: Biochimie - April 3, 2021 Category: Biochemistry Authors: Julieta Marrone Mauro Danielli C ésarI Gaspari Alejo M Capiglioni Ra úl A Marinelli Source Type: research

Effects of osmolality on the expression of brain aquaporins in AQP11-null mice
Biochimie. 2021 Mar 29:S0300-9084(21)00084-5. doi: 10.1016/j.biochi.2021.03.014. Online ahead of print.ABSTRACTWater transport in the brain is tightly controlled by blood-brain-barrier (BBB) composed of capillary endothelial cells expressing AQP1/AQP11 and glial foot processes expressing AQP4. Here we examined each AQP mRNA expression in acute hyponatremic and hypernatremic mouse models of wild type (WT) and AQP11 KO mice (KO). The expressions of AQP1, AQP4 and AQP11 mRNAs were quantified by real-time qRT-PCR analysis of whole brain RNA. Acute hyponatremia enhanced AQP4 expression without changing AQP1 expression in KO, wh...
Source: Biochimie - April 1, 2021 Category: Biochemistry Authors: Shin Koike Yasuko Tanaka Yoshiyuki Morishita Kenichi Ishibashi Source Type: research

DNA binding site kinetics of a large antiviral polyamide
Biochimie. 2021 Mar 29:S0300-9084(21)00085-7. doi: 10.1016/j.biochi.2021.03.015. Online ahead of print.ABSTRACTPolyamides (PAs) are powerful DNA ligands that can bind the minor groove of DNA with high affinity and specificity. While the characterization of PA-DNA behavior has focused principally on hairpin PAs 6-8 rings in size, there is increasing evidence that their behavior does not necessarily reflect the complexities that are emerging from studies of larger hairpin PAs, particularly concerning sequence mismatch tolerance and observed but unaddressed high PA-target site binding stoichiometries. To explore these complex...
Source: Biochimie - April 1, 2021 Category: Biochemistry Authors: Jacquelyn Niederschulte Yang Song Andrew H Park James K Bashkin Cynthia M Dupureur Source Type: research

Effects of osmolality on the expression of brain aquaporins in AQP11-null mice
Biochimie. 2021 Mar 29:S0300-9084(21)00084-5. doi: 10.1016/j.biochi.2021.03.014. Online ahead of print.ABSTRACTWater transport in the brain is tightly controlled by blood-brain-barrier (BBB) composed of capillary endothelial cells expressing AQP1/AQP11 and glial foot processes expressing AQP4. Here we examined each AQP mRNA expression in acute hyponatremic and hypernatremic mouse models of wild type (WT) and AQP11 KO mice (KO). The expressions of AQP1, AQP4 and AQP11 mRNAs were quantified by real-time qRT-PCR analysis of whole brain RNA. Acute hyponatremia enhanced AQP4 expression without changing AQP1 expression in KO, wh...
Source: Biochimie - April 1, 2021 Category: Biochemistry Authors: Shin Koike Yasuko Tanaka Yoshiyuki Morishita Kenichi Ishibashi Source Type: research

DNA binding site kinetics of a large antiviral polyamide
Biochimie. 2021 Mar 29:S0300-9084(21)00085-7. doi: 10.1016/j.biochi.2021.03.015. Online ahead of print.ABSTRACTPolyamides (PAs) are powerful DNA ligands that can bind the minor groove of DNA with high affinity and specificity. While the characterization of PA-DNA behavior has focused principally on hairpin PAs 6-8 rings in size, there is increasing evidence that their behavior does not necessarily reflect the complexities that are emerging from studies of larger hairpin PAs, particularly concerning sequence mismatch tolerance and observed but unaddressed high PA-target site binding stoichiometries. To explore these complex...
Source: Biochimie - April 1, 2021 Category: Biochemistry Authors: Jacquelyn Niederschulte Yang Song Andrew H Park James K Bashkin Cynthia M Dupureur Source Type: research

Cassava (Manihot esculenta) defensins: Prospection, structural analysis and tissue-specific expression under biotic/abiotic stresses
Biochimie. 2021 Mar 28:S0300-9084(21)00082-1. doi: 10.1016/j.biochi.2021.03.012. Online ahead of print.ABSTRACTDefensins are a prominent family of antimicrobial peptides. They play sophisticated roles in the defense against pathogens in all living organisms, but few works address their expression under different conditions and plant tissues. The present work prospected defensins of Manihot esculenta Crantz, popularly known as cassava. Five defensin candidates (MeDefs) were retrieved from the genome sequences and characterized. Considering chromosome distribution, only MeDef1 and 2 occupy adjacent positions in the same chro...
Source: Biochimie - March 31, 2021 Category: Biochemistry Authors: Carlos Andr é Dos Santos-Silva L ívia Maria Batista Vilela Roberta Lane de Oliveira Silva J éssica Barboza da Silva Alexandre Reis Machado Jo ão Pacífico Bezerra-Neto Sergio Crovella Ana Maria Benko-Iseppon Source Type: research

Cassava (Manihot esculenta) defensins: Prospection, structural analysis and tissue-specific expression under biotic/abiotic stresses
Biochimie. 2021 Mar 28:S0300-9084(21)00082-1. doi: 10.1016/j.biochi.2021.03.012. Online ahead of print.ABSTRACTDefensins are a prominent family of antimicrobial peptides. They play sophisticated roles in the defense against pathogens in all living organisms, but few works address their expression under different conditions and plant tissues. The present work prospected defensins of Manihot esculenta Crantz, popularly known as cassava. Five defensin candidates (MeDefs) were retrieved from the genome sequences and characterized. Considering chromosome distribution, only MeDef1 and 2 occupy adjacent positions in the same chro...
Source: Biochimie - March 31, 2021 Category: Biochemistry Authors: Carlos Andr é Dos Santos-Silva L ívia Maria Batista Vilela Roberta Lane de Oliveira Silva J éssica Barboza da Silva Alexandre Reis Machado Jo ão Pacífico Bezerra-Neto Sergio Crovella Ana Maria Benko-Iseppon Source Type: research

Poly(A) tail degradation in human cells: ATF4 mRNA as a model for biphasic deadenylation
Biochimie. 2021 Mar 25:S0300-9084(21)00083-3. doi: 10.1016/j.biochi.2021.03.013. Online ahead of print.ABSTRACTEukaryotic mRNA deadenylation is generally considered as a two-step process in which the PAN2-PAN3 complex initiates the poly(A) tail degradation while, in the second step, the CCR4-NOT complex completes deadenylation, leading to decapping and degradation of the mRNA body. However, the mechanism of the biphasic poly(A) tail deadenylation remains enigmatic in several points such as the timing of the switch between the two steps, the role of translation termination and the mRNAs population involved. Here, we have st...
Source: Biochimie - March 29, 2021 Category: Biochemistry Authors: B éatrice Jolles Olivier Jean-Jean Source Type: research

Poly(A) tail degradation in human cells: ATF4 mRNA as a model for biphasic deadenylation
Biochimie. 2021 Mar 25:S0300-9084(21)00083-3. doi: 10.1016/j.biochi.2021.03.013. Online ahead of print.ABSTRACTEukaryotic mRNA deadenylation is generally considered as a two-step process in which the PAN2-PAN3 complex initiates the poly(A) tail degradation while, in the second step, the CCR4-NOT complex completes deadenylation, leading to decapping and degradation of the mRNA body. However, the mechanism of the biphasic poly(A) tail deadenylation remains enigmatic in several points such as the timing of the switch between the two steps, the role of translation termination and the mRNAs population involved. Here, we have st...
Source: Biochimie - March 29, 2021 Category: Biochemistry Authors: B éatrice Jolles Olivier Jean-Jean Source Type: research

Poly(A) tail degradation in human cells: ATF4 mRNA as a model for biphasic deadenylation
Biochimie. 2021 Mar 25:S0300-9084(21)00083-3. doi: 10.1016/j.biochi.2021.03.013. Online ahead of print.ABSTRACTEukaryotic mRNA deadenylation is generally considered as a two-step process in which the PAN2-PAN3 complex initiates the poly(A) tail degradation while, in the second step, the CCR4-NOT complex completes deadenylation, leading to decapping and degradation of the mRNA body. However, the mechanism of the biphasic poly(A) tail deadenylation remains enigmatic in several points such as the timing of the switch between the two steps, the role of translation termination and the mRNAs population involved. Here, we have st...
Source: Biochimie - March 29, 2021 Category: Biochemistry Authors: B éatrice Jolles Olivier Jean-Jean Source Type: research

trans-Anethole attenuated renal injury and reduced expressions of angiotensin II receptor (AT1R) and TGF- β in streptozotocin-induced diabetic rats
Biochimie. 2021 Mar 23:S0300-9084(21)00081-X. doi: 10.1016/j.biochi.2021.03.011. Online ahead of print.ABSTRACTFibrosis is a pathological process in diabetic nephropathy that causes renal failure and dysfunction. Given the known anti-diabetic effects of trans-Anethole (TA), we aimed to investigate its renoprotective and anti-fibrotic effect alone and in combination with losartan in diabetic nephropathy. Male Wistar rats received a single intraperitoneal injection of 65 mg/kg streptozotocin (STZ) for diabetes induction. Diabetic rats were treated orally with saline, TA (80 mg/kg), losartan (Los; 10 mg/kg), or the combinatio...
Source: Biochimie - March 27, 2021 Category: Biochemistry Authors: Zahra Samadi-Noshahr Alireza Ebrahimzadeh-Bideskan Mosa-Al-Reza Hadjzadeh Mohammad Naser Shafei Hossein Salmani Sara Hosseinian Abolfazl Khajavi-Rad Source Type: research

DHEA-pretreatment attenuates oxidative stress in kidney-cortex and liver of diabetic rabbits and delays development of the disease
In conclusion, the current investigation underscores the complexity of the antioxidant action of DHEA. The data are of clinical interest since DHEA supplementation could prevent the deleterious effects of ROS and delay, or even prevent the onset of many diseases. However, in view of the reported pro-oxidant effects of high DHEA doses, the potential use of this agent as a supplement needs a careful evaluation.PMID:33771656 | DOI:10.1016/j.biochi.2021.03.010 (Source: Biochimie)
Source: Biochimie - March 27, 2021 Category: Biochemistry Authors: Anna Kiersztan Kongorzul Gaanga Apolonia Witecka Adam K Jagielski Source Type: research

trans-Anethole attenuated renal injury and reduced expressions of angiotensin II receptor (AT1R) and TGF- β in streptozotocin-induced diabetic rats
Biochimie. 2021 Mar 23:S0300-9084(21)00081-X. doi: 10.1016/j.biochi.2021.03.011. Online ahead of print.ABSTRACTFibrosis is a pathological process in diabetic nephropathy that causes renal failure and dysfunction. Given the known anti-diabetic effects of trans-Anethole (TA), we aimed to investigate its renoprotective and anti-fibrotic effect alone and in combination with losartan in diabetic nephropathy. Male Wistar rats received a single intraperitoneal injection of 65 mg/kg streptozotocin (STZ) for diabetes induction. Diabetic rats were treated orally with saline, TA (80 mg/kg), losartan (Los; 10 mg/kg), or the combinatio...
Source: Biochimie - March 27, 2021 Category: Biochemistry Authors: Zahra Samadi-Noshahr Alireza Ebrahimzadeh-Bideskan Mosa-Al-Reza Hadjzadeh Mohammad Naser Shafei Hossein Salmani Sara Hosseinian Abolfazl Khajavi-Rad Source Type: research

DHEA-pretreatment attenuates oxidative stress in kidney-cortex and liver of diabetic rabbits and delays development of the disease
In conclusion, the current investigation underscores the complexity of the antioxidant action of DHEA. The data are of clinical interest since DHEA supplementation could prevent the deleterious effects of ROS and delay, or even prevent the onset of many diseases. However, in view of the reported pro-oxidant effects of high DHEA doses, the potential use of this agent as a supplement needs a careful evaluation.PMID:33771656 | DOI:10.1016/j.biochi.2021.03.010 (Source: Biochimie)
Source: Biochimie - March 27, 2021 Category: Biochemistry Authors: Anna Kiersztan Kongorzul Gaanga Apolonia Witecka Adam K Jagielski Source Type: research

Role of pyroptosis in cancer cells and clinical applications
Biochimie. 2021 Mar 18;185:78-86. doi: 10.1016/j.biochi.2021.03.007. Online ahead of print.ABSTRACTChemotherapy drugs usually inhibit tumor cell growth through the apoptosis pathway. However, tumor cells become resistant to chemotherapy drugs by evading apoptosis. It is necessary to find new ways to inhibit tumor growth through other types of death. Pyroptosis is a recently identified inflammatory cell death that plays an important role in a variety of diseases, including cancer. In this review, we will systematically review recent progress in the pyroptosis signaling pathway, the role of inflammasomes in cancer in the con...
Source: Biochimie - March 22, 2021 Category: Biochemistry Authors: Xiaoli Ju Zhilong Yang Heng Zhang Qiang Wang Source Type: research

The involvement of Nile tilapia (Oreochromis niloticus) Neu4 sialidase in neural differentiation during early ontogenesis
Biochimie. 2021 Mar 18:S0300-9084(21)00078-X. doi: 10.1016/j.biochi.2021.03.008. Online ahead of print.ABSTRACTNeurogenesis is an important process for the formation of the central nervous system during ontogenesis. Mammalian sialidases are involved in neurogenesis through desialylation of sialo-glycoconjugates. However, the significance of fish sialidases, unlike that of mammals, in neurogenesis has not been investigated. The present study focuses on Nile tilapia (Oreochromis niloticus) because of its unique profiles of sialidases related to enzymatic properties, subcellular localization, and tissue-specific gene expressi...
Source: Biochimie - March 22, 2021 Category: Biochemistry Authors: Akinobu Honda Oki Hayasaka Kenjiro Mio Koji Fujimura Tomonari Kotani Masaharu Komatsu Kazuhiro Shiozaki Source Type: research

Structure and activation mechanism of the human liver-type glutaminase GLS2
Biochimie. 2021 Mar 18:S0300-9084(21)00079-1. doi: 10.1016/j.biochi.2021.03.009. Online ahead of print.ABSTRACTCancer cells exhibit an altered metabolic phenotype, consuming higher levels of the amino acid glutamine. This metabolic reprogramming depends on increased mitochondrial glutaminase activity to convert glutamine to glutamate, an essential precursor for bioenergetic and biosynthetic processes in cells. Mammals encode the kidney-type (GLS) and liver-type (GLS2) glutaminase isozymes. GLS is overexpressed in cancer and associated with enhanced malignancy. On the other hand, GLS2 is either a tumor suppressor or an onco...
Source: Biochimie - March 22, 2021 Category: Biochemistry Authors: Igor M Ferreira Jos é Edwin N Quesñay Alliny Cs Bastos Camila T Rodrigues Melanie Vollmar Tobias Krojer Claire Strain-Damerell Nicola A Burgess-Brown Frank von Delft Wyatt W Yue Sandra Mg Dias Andre Lb Ambrosio Source Type: research

Role of pyroptosis in cancer cells and clinical applications
Biochimie. 2021 Mar 18;185:78-86. doi: 10.1016/j.biochi.2021.03.007. Online ahead of print.ABSTRACTChemotherapy drugs usually inhibit tumor cell growth through the apoptosis pathway. However, tumor cells become resistant to chemotherapy drugs by evading apoptosis. It is necessary to find new ways to inhibit tumor growth through other types of death. Pyroptosis is a recently identified inflammatory cell death that plays an important role in a variety of diseases, including cancer. In this review, we will systematically review recent progress in the pyroptosis signaling pathway, the role of inflammasomes in cancer in the con...
Source: Biochimie - March 22, 2021 Category: Biochemistry Authors: Xiaoli Ju Zhilong Yang Heng Zhang Qiang Wang Source Type: research

The involvement of Nile tilapia (Oreochromis niloticus) Neu4 sialidase in neural differentiation during early ontogenesis
Biochimie. 2021 Mar 18:S0300-9084(21)00078-X. doi: 10.1016/j.biochi.2021.03.008. Online ahead of print.ABSTRACTNeurogenesis is an important process for the formation of the central nervous system during ontogenesis. Mammalian sialidases are involved in neurogenesis through desialylation of sialo-glycoconjugates. However, the significance of fish sialidases, unlike that of mammals, in neurogenesis has not been investigated. The present study focuses on Nile tilapia (Oreochromis niloticus) because of its unique profiles of sialidases related to enzymatic properties, subcellular localization, and tissue-specific gene expressi...
Source: Biochimie - March 22, 2021 Category: Biochemistry Authors: Akinobu Honda Oki Hayasaka Kenjiro Mio Koji Fujimura Tomonari Kotani Masaharu Komatsu Kazuhiro Shiozaki Source Type: research

Structure and activation mechanism of the human liver-type glutaminase GLS2
Biochimie. 2021 Mar 18:S0300-9084(21)00079-1. doi: 10.1016/j.biochi.2021.03.009. Online ahead of print.ABSTRACTCancer cells exhibit an altered metabolic phenotype, consuming higher levels of the amino acid glutamine. This metabolic reprogramming depends on increased mitochondrial glutaminase activity to convert glutamine to glutamate, an essential precursor for bioenergetic and biosynthetic processes in cells. Mammals encode the kidney-type (GLS) and liver-type (GLS2) glutaminase isozymes. GLS is overexpressed in cancer and associated with enhanced malignancy. On the other hand, GLS2 is either a tumor suppressor or an onco...
Source: Biochimie - March 22, 2021 Category: Biochemistry Authors: Igor M Ferreira Jos é Edwin N Quesñay Alliny Cs Bastos Camila T Rodrigues Melanie Vollmar Tobias Krojer Claire Strain-Damerell Nicola A Burgess-Brown Frank von Delft Wyatt W Yue Sandra Mg Dias Andre Lb Ambrosio Source Type: research

Role of pyroptosis in cancer cells and clinical applications
Biochimie. 2021 Mar 18;185:78-86. doi: 10.1016/j.biochi.2021.03.007. Online ahead of print.ABSTRACTChemotherapy drugs usually inhibit tumor cell growth through the apoptosis pathway. However, tumor cells become resistant to chemotherapy drugs by evading apoptosis. It is necessary to find new ways to inhibit tumor growth through other types of death. Pyroptosis is a recently identified inflammatory cell death that plays an important role in a variety of diseases, including cancer. In this review, we will systematically review recent progress in the pyroptosis signaling pathway, the role of inflammasomes in cancer in the con...
Source: Biochimie - March 22, 2021 Category: Biochemistry Authors: Xiaoli Ju Zhilong Yang Heng Zhang Qiang Wang Source Type: research

The involvement of Nile tilapia (Oreochromis niloticus) Neu4 sialidase in neural differentiation during early ontogenesis
Biochimie. 2021 Mar 18:S0300-9084(21)00078-X. doi: 10.1016/j.biochi.2021.03.008. Online ahead of print.ABSTRACTNeurogenesis is an important process for the formation of the central nervous system during ontogenesis. Mammalian sialidases are involved in neurogenesis through desialylation of sialo-glycoconjugates. However, the significance of fish sialidases, unlike that of mammals, in neurogenesis has not been investigated. The present study focuses on Nile tilapia (Oreochromis niloticus) because of its unique profiles of sialidases related to enzymatic properties, subcellular localization, and tissue-specific gene expressi...
Source: Biochimie - March 22, 2021 Category: Biochemistry Authors: Akinobu Honda Oki Hayasaka Kenjiro Mio Koji Fujimura Tomonari Kotani Masaharu Komatsu Kazuhiro Shiozaki Source Type: research

Structure and activation mechanism of the human liver-type glutaminase GLS2
Biochimie. 2021 Mar 18:S0300-9084(21)00079-1. doi: 10.1016/j.biochi.2021.03.009. Online ahead of print.ABSTRACTCancer cells exhibit an altered metabolic phenotype, consuming higher levels of the amino acid glutamine. This metabolic reprogramming depends on increased mitochondrial glutaminase activity to convert glutamine to glutamate, an essential precursor for bioenergetic and biosynthetic processes in cells. Mammals encode the kidney-type (GLS) and liver-type (GLS2) glutaminase isozymes. GLS is overexpressed in cancer and associated with enhanced malignancy. On the other hand, GLS2 is either a tumor suppressor or an onco...
Source: Biochimie - March 22, 2021 Category: Biochemistry Authors: Igor M Ferreira Jos é Edwin N Quesñay Alliny Cs Bastos Camila T Rodrigues Melanie Vollmar Tobias Krojer Claire Strain-Damerell Nicola A Burgess-Brown Frank von Delft Wyatt W Yue Sandra Mg Dias Andre Lb Ambrosio Source Type: research

Role of pyroptosis in cancer cells and clinical applications
Biochimie. 2021 Mar 18:S0300-9084(21)00077-8. doi: 10.1016/j.biochi.2021.03.007. Online ahead of print.ABSTRACTChemotherapy drugs usually inhibit tumor cell growth through the apoptosis pathway. However, tumor cells become resistant to chemotherapy drugs by evading apoptosis. It is necessary to find new ways to inhibit tumor growth through other types of death. Pyroptosis is a recently identified inflammatory cell death that plays an important role in a variety of diseases, including cancer. In this review, we will systematically review recent progress in the pyroptosis signaling pathway, the role of inflammasomes in cance...
Source: Biochimie - March 22, 2021 Category: Biochemistry Authors: Xiaoli Ju Zhilong Yang Heng Zhang Qiang Wang Source Type: research

The involvement of Nile tilapia (Oreochromis niloticus) Neu4 sialidase in neural differentiation during early ontogenesis
Biochimie. 2021 Mar 18:S0300-9084(21)00078-X. doi: 10.1016/j.biochi.2021.03.008. Online ahead of print.ABSTRACTNeurogenesis is an important process for the formation of the central nervous system during ontogenesis. Mammalian sialidases are involved in neurogenesis through desialylation of sialo-glycoconjugates. However, the significance of fish sialidases, unlike that of mammals, in neurogenesis has not been investigated. The present study focuses on Nile tilapia (Oreochromis niloticus) because of its unique profiles of sialidases related to enzymatic properties, subcellular localization, and tissue-specific gene expressi...
Source: Biochimie - March 22, 2021 Category: Biochemistry Authors: Akinobu Honda Oki Hayasaka Kenjiro Mio Koji Fujimura Tomonari Kotani Masaharu Komatsu Kazuhiro Shiozaki Source Type: research

Structure and activation mechanism of the human liver-type glutaminase GLS2
Biochimie. 2021 Mar 18:S0300-9084(21)00079-1. doi: 10.1016/j.biochi.2021.03.009. Online ahead of print.ABSTRACTCancer cells exhibit an altered metabolic phenotype, consuming higher levels of the amino acid glutamine. This metabolic reprogramming depends on increased mitochondrial glutaminase activity to convert glutamine to glutamate, an essential precursor for bioenergetic and biosynthetic processes in cells. Mammals encode the kidney-type (GLS) and liver-type (GLS2) glutaminase isozymes. GLS is overexpressed in cancer and associated with enhanced malignancy. On the other hand, GLS2 is either a tumor suppressor or an onco...
Source: Biochimie - March 22, 2021 Category: Biochemistry Authors: Igor M Ferreira Jos é Edwin N Quesñay Alliny Cs Bastos Camila T Rodrigues Melanie Vollmar Tobias Krojer Claire Strain-Damerell Nicola A Burgess-Brown Frank von Delft Wyatt W Yue Sandra Mg Dias Andre Lb Ambrosio Source Type: research

Quench me if you can: Alpha-2-macroglobulin trypsin complexes enable serum biomarker analysis by MALDI mass spectrometry
Biochimie. 2021 Mar 17:S0300-9084(21)00075-4. doi: 10.1016/j.biochi.2021.03.005. Online ahead of print.ABSTRACTOne of the main functions of alpha-2-macroglobulin (A2M) in human blood serum is the binding of all classes of protease. It is known that trypsin, after such interaction, possesses modified proteolytic activity. Trypsin first hydrolyzes two bonds in A2M's 'bait region', and the peptide 705VGFYESDVMGR715 is released from A2M. In this work, specifics of the A2M-trypsin interaction were used to determine A2M concentration directly in human blood serum using MALDI mass-spectrometry. Following exogenous addition of try...
Source: Biochimie - March 21, 2021 Category: Biochemistry Authors: Aleksandr S Taraskin Konstantin K Semenov Aleksandr V Protasov Alexey A Lozhkov Alexandr A Tyulin Aram A Shaldzhyan Edward S Ramsay Olga A Mirgorodskaya Sergey A Klotchenko Yana A Zabrodskaya Source Type: research

Quench me if you can: Alpha-2-macroglobulin trypsin complexes enable serum biomarker analysis by MALDI mass spectrometry
Biochimie. 2021 Mar 17:S0300-9084(21)00075-4. doi: 10.1016/j.biochi.2021.03.005. Online ahead of print.ABSTRACTOne of the main functions of alpha-2-macroglobulin (A2M) in human blood serum is the binding of all classes of protease. It is known that trypsin, after such interaction, possesses modified proteolytic activity. Trypsin first hydrolyzes two bonds in A2M's 'bait region', and the peptide 705VGFYESDVMGR715 is released from A2M. In this work, specifics of the A2M-trypsin interaction were used to determine A2M concentration directly in human blood serum using MALDI mass-spectrometry. Following exogenous addition of try...
Source: Biochimie - March 21, 2021 Category: Biochemistry Authors: Aleksandr S Taraskin Konstantin K Semenov Aleksandr V Protasov Alexey A Lozhkov Alexandr A Tyulin Aram A Shaldzhyan Edward S Ramsay Olga A Mirgorodskaya Sergey A Klotchenko Yana A Zabrodskaya Source Type: research

Metal effect on intein splicing: A review
Biochimie. 2021 Mar 13:S0300-9084(21)00076-6. doi: 10.1016/j.biochi.2021.03.006. Online ahead of print.ABSTRACTInteins are intervening polypeptides that interrupt the functional domains of several important proteins across the three domains of life. Inteins excise themselves from the precursor protein, ligating concomitant extein residues in a process called protein splicing. Post-translational auto-removal of inteins remain critical for the generation of active proteins. The perspective of inteins in science is a robust field of research, however fundamental studies centralized upon splicing regulatory mechanism are imper...
Source: Biochimie - March 17, 2021 Category: Biochemistry Authors: Sunita Panda Ananya Nanda Sourya Subhra Nasker Debjani Sen Ashwaria Mehra Sasmita Nayak Source Type: research

NMS-873 functions as a dual inhibitor of mitochondrial oxidative phosphorylation
In this study we describe our serendipitous discovery that NMS-873, a compound that was previously identified as a highly selective allosteric inhibitor of the ATPase valosin-containing protein (VCP/p97), rapidly induces aerobic fermentation in cultured human and mouse cells. Our further investigation uncovered an unexpected off-target effect of NMS-873 on mitochondrial oxidative phosphorylation, specifically as a dual inhibitor of Complex I and ATP synthase. This work points to the need for caution regarding the interpretation of cell survival data associated with NMS-873 treatment and indicates that cellular toxicity ass...
Source: Biochimie - March 17, 2021 Category: Biochemistry Authors: Miranda F Bouwer Kathryn E Hamilton Patrick B Jonker Sam R Kuiper Larry L Louters Brendan D Looyenga Source Type: research

Biochemical characterization of a unique DNA polymerase A from the extreme radioresistant organism Deinococcus radiodurans
In this study, we systematically examined the enzymatic activities of DrPolA and characterized its unique features. DrPolA contains an N-terminal nuclease domain (DrPolA-NTD) and a C-terminal Klenow fragment (KlenDr). Compared with the Klenow fragment of E. coli Pol I, KlenDr shows higher fidelity despite the lacking of 3'-5' exonuclease proofreading activity and prefers double-strand DNA rather than Primer-Template substrates. Apart from the well-annotated 5'-3' exonuclease and flap endonuclease activities, DrPolA-NTD displays approximately 140-fold higher gap endonuclease activity than its homolog in E. coli and Human FE...
Source: Biochimie - March 17, 2021 Category: Biochemistry Authors: Xingru Zhou Xuanyi Chen Ying An Huizhi Lu Liangyan Wang Hong Xu Bing Tian Ye Zhao Yuejin Hua Source Type: research

Metal effect on intein splicing: A review
Biochimie. 2021 Mar 13:S0300-9084(21)00076-6. doi: 10.1016/j.biochi.2021.03.006. Online ahead of print.ABSTRACTInteins are intervening polypeptides that interrupt the functional domains of several important proteins across the three domains of life. Inteins excise themselves from the precursor protein, ligating concomitant extein residues in a process called protein splicing. Post-translational auto-removal of inteins remain critical for the generation of active proteins. The perspective of inteins in science is a robust field of research, however fundamental studies centralized upon splicing regulatory mechanism are imper...
Source: Biochimie - March 17, 2021 Category: Biochemistry Authors: Sunita Panda Ananya Nanda Sourya Subhra Nasker Debjani Sen Ashwaria Mehra Sasmita Nayak Source Type: research

NMS-873 functions as a dual inhibitor of mitochondrial oxidative phosphorylation
In this study we describe our serendipitous discovery that NMS-873, a compound that was previously identified as a highly selective allosteric inhibitor of the ATPase valosin-containing protein (VCP/p97), rapidly induces aerobic fermentation in cultured human and mouse cells. Our further investigation uncovered an unexpected off-target effect of NMS-873 on mitochondrial oxidative phosphorylation, specifically as a dual inhibitor of Complex I and ATP synthase. This work points to the need for caution regarding the interpretation of cell survival data associated with NMS-873 treatment and indicates that cellular toxicity ass...
Source: Biochimie - March 17, 2021 Category: Biochemistry Authors: Miranda F Bouwer Kathryn E Hamilton Patrick B Jonker Sam R Kuiper Larry L Louters Brendan D Looyenga Source Type: research

Biochemical characterization of a unique DNA polymerase A from the extreme radioresistant organism Deinococcus radiodurans
In this study, we systematically examined the enzymatic activities of DrPolA and characterized its unique features. DrPolA contains an N-terminal nuclease domain (DrPolA-NTD) and a C-terminal Klenow fragment (KlenDr). Compared with the Klenow fragment of E. coli Pol I, KlenDr shows higher fidelity despite the lacking of 3'-5' exonuclease proofreading activity and prefers double-strand DNA rather than Primer-Template substrates. Apart from the well-annotated 5'-3' exonuclease and flap endonuclease activities, DrPolA-NTD displays approximately 140-fold higher gap endonuclease activity than its homolog in E. coli and Human FE...
Source: Biochimie - March 17, 2021 Category: Biochemistry Authors: Xingru Zhou Xuanyi Chen Ying An Huizhi Lu Liangyan Wang Hong Xu Bing Tian Ye Zhao Yuejin Hua Source Type: research

Metal effect on intein splicing: A review
Biochimie. 2021 Mar 13:S0300-9084(21)00076-6. doi: 10.1016/j.biochi.2021.03.006. Online ahead of print.ABSTRACTInteins are intervening polypeptides that interrupt the functional domains of several important proteins across the three domains of life. Inteins excise themselves from the precursor protein, ligating concomitant extein residues in a process called protein splicing. Post-translational auto-removal of inteins remain critical for the generation of active proteins. The perspective of inteins in science is a robust field of research, however fundamental studies centralized upon splicing regulatory mechanism are imper...
Source: Biochimie - March 17, 2021 Category: Biochemistry Authors: Sunita Panda Ananya Nanda Sourya Subhra Nasker Debjani Sen Ashwaria Mehra Sasmita Nayak Source Type: research

NMS-873 functions as a dual inhibitor of mitochondrial oxidative phosphorylation
In this study we describe our serendipitous discovery that NMS-873, a compound that was previously identified as a highly selective allosteric inhibitor of the ATPase valosin-containing protein (VCP/p97), rapidly induces aerobic fermentation in cultured human and mouse cells. Our further investigation uncovered an unexpected off-target effect of NMS-873 on mitochondrial oxidative phosphorylation, specifically as a dual inhibitor of Complex I and ATP synthase. This work points to the need for caution regarding the interpretation of cell survival data associated with NMS-873 treatment and indicates that cellular toxicity ass...
Source: Biochimie - March 17, 2021 Category: Biochemistry Authors: Miranda F Bouwer Kathryn E Hamilton Patrick B Jonker Sam R Kuiper Larry L Louters Brendan D Looyenga Source Type: research

Biochemical characterization of a unique DNA polymerase A from the extreme radioresistant organism Deinococcus radiodurans
In this study, we systematically examined the enzymatic activities of DrPolA and characterized its unique features. DrPolA contains an N-terminal nuclease domain (DrPolA-NTD) and a C-terminal Klenow fragment (KlenDr). Compared with the Klenow fragment of E. coli Pol I, KlenDr shows higher fidelity despite the lacking of 3'-5' exonuclease proofreading activity and prefers double-strand DNA rather than Primer-Template substrates. Apart from the well-annotated 5'-3' exonuclease and flap endonuclease activities, DrPolA-NTD displays approximately 140-fold higher gap endonuclease activity than its homolog in E. coli and Human FE...
Source: Biochimie - March 17, 2021 Category: Biochemistry Authors: Xingru Zhou Xuanyi Chen Ying An Huizhi Lu Liangyan Wang Hong Xu Bing Tian Ye Zhao Yuejin Hua Source Type: research

Metal effect on intein splicing: A review
Biochimie. 2021 Mar 13:S0300-9084(21)00076-6. doi: 10.1016/j.biochi.2021.03.006. Online ahead of print.ABSTRACTInteins are intervening polypeptides that interrupt the functional domains of several important proteins across the three domains of life. Inteins excise themselves from the precursor protein, ligating concomitant extein residues in a process called protein splicing. Post-translational auto-removal of inteins remain critical for the generation of active proteins. The perspective of inteins in science is a robust field of research, however fundamental studies centralized upon splicing regulatory mechanism are imper...
Source: Biochimie - March 17, 2021 Category: Biochemistry Authors: Sunita Panda Ananya Nanda Sourya Subhra Nasker Debjani Sen Ashwaria Mehra Sasmita Nayak Source Type: research