Isolation of the AccCDK8 gene of Apis cerana cerana and its functional analysis under pesticide and heavy metal stress
Biochimie. 2023 Sep 11:S0300-9084(23)00239-0. doi: 10.1016/j.biochi.2023.09.012. Online ahead of print.ABSTRACTEnvironmental pollution has gained negative attention in recent years. The pesticides and heavy metals are top list of environmental toxicants directly endangering the survival and development of Apis cerana cerana. Cyclin-dependent kinases (CDKs) are heteromeric serine/threonine kinases that participate in cell cycle regulation and have a vital role in pesticide and heavy metal stress in Apis cerana cerana. In this experiment, we filtered out CDK8 gene from Apis cerana cerana (AccCDK8) and investigated its functi...
Source: Biochimie - September 13, 2023 Category: Biochemistry Authors: Jing Li Dezheng Guo Jinhao Bai Hongfang Wang Chen Wang Ying Wang Xingqi Guo Baohua Xu Zhenguo Liu Source Type: research
Dissolved metal ions and mineral-liposome hybrid systems: Underlying interactions, synthesis, and characterization
Biochimie. 2023 Sep 10:S0300-9084(23)00218-3. doi: 10.1016/j.biochi.2023.09.009. Online ahead of print.ABSTRACTLiposomes are versatile lipid-based vesicles with interesting physicochemical properties, making them excellent candidates for interdisciplinary applications in the medicinal, biological, and environmental sciences. The synthesis of mineral-liposome hybrid systems lends normally inert vesicles with the catalytic, magnetic, electrical, and optical properties of the integrated mineral species. Such applications require an understanding of the physicochemical interactions between organic molecules and inorganic cryst...
Source: Biochimie - September 12, 2023 Category: Biochemistry Authors: Nil Gaudu Orion Farr Georges Ona-Nguema Simon Duval Source Type: research
Dissolved metal ions and mineral-liposome hybrid systems: Underlying interactions, synthesis, and characterization
Biochimie. 2023 Sep 10:S0300-9084(23)00218-3. doi: 10.1016/j.biochi.2023.09.009. Online ahead of print.ABSTRACTLiposomes are versatile lipid-based vesicles with interesting physicochemical properties, making them excellent candidates for interdisciplinary applications in the medicinal, biological, and environmental sciences. The synthesis of mineral-liposome hybrid systems lends normally inert vesicles with the catalytic, magnetic, electrical, and optical properties of the integrated mineral species. Such applications require an understanding of the physicochemical interactions between organic molecules and inorganic cryst...
Source: Biochimie - September 12, 2023 Category: Biochemistry Authors: Nil Gaudu Orion Farr Georges Ona-Nguema Simon Duval Source Type: research
Targeting of CRISPR-Cas12a crRNAs into human mitochondria
Biochimie. 2023 Sep 8:S0300-9084(23)00215-8. doi: 10.1016/j.biochi.2023.09.006. Online ahead of print.ABSTRACTMitochondrial gene editing holds great promise as a therapeutic approach for mitochondrial diseases caused by mutations in the mitochondrial DNA (mtDNA). Current strategies focus on reducing mutant mtDNA heteroplasmy levels through targeted cleavage or base editing. However, the delivery of editing components into mitochondria remains a challenge. Here we investigate the import of CRISPR-Cas12a system guide RNAs (crRNAs) into human mitochondria and study the structural requirements for this process by northern blot...
Source: Biochimie - September 10, 2023 Category: Biochemistry Authors: Natalia Nikitchina Egor Ulashchik Vadim Shmanai Anne-Marie Heckel Ivan Tarassov Ilya Mazunin Nina Entelis Source Type: research
Targeting of CRISPR-Cas12a crRNAs into human mitochondria
Biochimie. 2023 Sep 8:S0300-9084(23)00215-8. doi: 10.1016/j.biochi.2023.09.006. Online ahead of print.ABSTRACTMitochondrial gene editing holds great promise as a therapeutic approach for mitochondrial diseases caused by mutations in the mitochondrial DNA (mtDNA). Current strategies focus on reducing mutant mtDNA heteroplasmy levels through targeted cleavage or base editing. However, the delivery of editing components into mitochondria remains a challenge. Here we investigate the import of CRISPR-Cas12a system guide RNAs (crRNAs) into human mitochondria and study the structural requirements for this process by northern blot...
Source: Biochimie - September 10, 2023 Category: Biochemistry Authors: Natalia Nikitchina Egor Ulashchik Vadim Shmanai Anne-Marie Heckel Ivan Tarassov Ilya Mazunin Nina Entelis Source Type: research
