Anti-Acanthamoeba metallopharmaceuticals: Amoebicidal activity and synergistic effect of copper(II) coordination compound
This study evaluates the amoebicidal activity of copper(II) coordination compounds in combination with chlorhexidine and the cytotoxicity to topical ocular application. These copper(II) coordination compounds were screened against Acanthamoeba castellanii trophozoites (ATCC 50492). The cytotoxicity on rabbit corneal cell line (ATCC-CCL 60) was performed. The compounds showed high amoebicidal potential, with inhibition of trophozoite viability above 80%. The Cp12 and Cp13 compounds showed Minimal Inhibitory Amoebicidal Concentration (MIAC) at 200 µM and mean inhibitory concentration (IC50) values lower than 10 µM. Against...
Source: Biometals - April 22, 2024 Category: Biochemistry Authors: Jaqueline da Silveira Ana Paula Cardoso Christiane Fernandes Adolfo Horn Junior Gabriella da Rosa Monte Machado Karin Silva Caumo Source Type: research
Anti-Acanthamoeba metallopharmaceuticals: Amoebicidal activity and synergistic effect of copper(II) coordination compound
This study evaluates the amoebicidal activity of copper(II) coordination compounds in combination with chlorhexidine and the cytotoxicity to topical ocular application. These copper(II) coordination compounds were screened against Acanthamoeba castellanii trophozoites (ATCC 50492). The cytotoxicity on rabbit corneal cell line (ATCC-CCL 60) was performed. The compounds showed high amoebicidal potential, with inhibition of trophozoite viability above 80%. The Cp12 and Cp13 compounds showed Minimal Inhibitory Amoebicidal Concentration (MIAC) at 200 µM and mean inhibitory concentration (IC50) values lower than 10 µM. Against...
Source: Biometals - April 22, 2024 Category: Biochemistry Authors: Jaqueline da Silveira Ana Paula Cardoso Christiane Fernandes Adolfo Horn Junior Gabriella da Rosa Monte Machado Karin Silva Caumo Source Type: research
Anti-Acanthamoeba metallopharmaceuticals: Amoebicidal activity and synergistic effect of copper(II) coordination compound
This study evaluates the amoebicidal activity of copper(II) coordination compounds in combination with chlorhexidine and the cytotoxicity to topical ocular application. These copper(II) coordination compounds were screened against Acanthamoeba castellanii trophozoites (ATCC 50492). The cytotoxicity on rabbit corneal cell line (ATCC-CCL 60) was performed. The compounds showed high amoebicidal potential, with inhibition of trophozoite viability above 80%. The Cp12 and Cp13 compounds showed Minimal Inhibitory Amoebicidal Concentration (MIAC) at 200 µM and mean inhibitory concentration (IC50) values lower than 10 µM. Against...
Source: Biometals - April 22, 2024 Category: Biochemistry Authors: Jaqueline da Silveira Ana Paula Cardoso Christiane Fernandes Adolfo Horn Junior Gabriella da Rosa Monte Machado Karin Silva Caumo Source Type: research
Anti-Acanthamoeba metallopharmaceuticals: Amoebicidal activity and synergistic effect of copper(II) coordination compound
This study evaluates the amoebicidal activity of copper(II) coordination compounds in combination with chlorhexidine and the cytotoxicity to topical ocular application. These copper(II) coordination compounds were screened against Acanthamoeba castellanii trophozoites (ATCC 50492). The cytotoxicity on rabbit corneal cell line (ATCC-CCL 60) was performed. The compounds showed high amoebicidal potential, with inhibition of trophozoite viability above 80%. The Cp12 and Cp13 compounds showed Minimal Inhibitory Amoebicidal Concentration (MIAC) at 200 µM and mean inhibitory concentration (IC50) values lower than 10 µM. Against...
Source: Biometals - April 22, 2024 Category: Biochemistry Authors: Jaqueline da Silveira Ana Paula Cardoso Christiane Fernandes Adolfo Horn Junior Gabriella da Rosa Monte Machado Karin Silva Caumo Source Type: research
Evidence for persistent UV-induced DNA damage and altered DNA damage response in xeroderma pigmentosa patient corneas
Exp Eye Res. 2024 Apr 17:109901. doi: 10.1016/j.exer.2024.109901. Online ahead of print.ABSTRACTXeroderma pigmentosum (XP) is a rare genetic disorder characterized by injury to the ocular surface due to exposure to ultraviolet (UV) radiation. UV-induced damage in the cells leads to the formation of cyclobutane pyrimidine dimers (CPDs) and 6-4 pyrimidine-pyrimidone photoproducts that are repaired by the NER (Nucleotide Excision Repair) pathway. Mutations in the genes coding for NER proteins, as reported in XP patients, would lead to sub-optimal damage repair resulting in clinical signs varying from photo-keratitis to cancer...
Source: Experimental Eye Research - April 19, 2024 Category: Opthalmology Authors: Jacquelyn Akepogu Saumya Jakati Sunita Chaurasia Charanya Ramachandran Source Type: research
Femtosecond thin-flap laser assisted in situ keratomileusis for correction of post-penetrating keratoplasty ametropia: long-term outcome
To evaluate the long-term clinical outcomes of femtosecond thin-flap LASIK (femto-LASIK) for correction of refractive error after penetrating keratoplasty in keratoconus-affected eyes. (Source: BMC Ophthalmology)
Source: BMC Ophthalmology - April 16, 2024 Category: Opthalmology Authors: Bahram Einollahi, Javad Rezaei, Mohammad-Mehdi Sadoughi, Sepehr Feizi, Neda Einollahi, Amir Reza Veisi and Kiana Hassanpour Tags: Research Source Type: research
Effect of keratoplasty on the quality of life of keratoconus patients
In this study, we compare the quality of life of patients with keratoconus in the early (stages 1 and 2) or advanced (stages 3 and 4) stages of the Krumeich classification, as well as patients who have had keratoconus treated by keratoplasty. Quality of life was assessed using the NEI-VFQ 25 questionnaire, the most widely used for keratoconus. An aberrometric examination (OQAS®; HD Analyser, Visiometrics, Terrassa, Spain) was also performed to assess patients' quality of vision. Our results show that keratoplasty provides an improvement in quality of life compared with advanced-stage keratoconus in the areas of distance (...
Source: Journal Francais d Ophtalmologie - April 12, 2024 Category: Opthalmology Authors: T Jurkiewicz A-S Marty C Febvay C Burillon Source Type: research
How to tame a tight DMEK-roll? The `bubble-in-the-roll` technique
Since its introduction in 2006, Descemet Membrane Endothelial Keratoplasty (DMEK) has become the surgical procedure of choice for endothelial failure.1 Most surgeons follow the `no-touch` surgical technique.2 Briefly: DMEK-rolls delivered in organ culture medium are rinsed with balance salt solution (BSS, Alcon Nederland BV, Gorinchem, Nederland) and stained two times with trypan blue 0.06% (Vision Blue, D.O.R.C. International, The Netherlands). A direct flow of BSS is used on top of the DMEK graft to open up the roll and to create a double roll. (Source: American Journal of Ophthalmology)
Source: American Journal of Ophthalmology - April 12, 2024 Category: Opthalmology Authors: Gergely Losonczy Tags: Illustrations & Insights Source Type: research
How to tame a tight DMEK-roll? The ‘bubble-in-the-roll’ technique
Since its introduction in 2006, Descemet Membrane Endothelial Keratoplasty (DMEK) has become the surgical procedure of choice for endothelial failure.1 Most surgeons follow the ‘no-touch’ surgical technique.2 Briefly: DMEK-rolls delivered in organ culture medium are rinsed with balance salt solution (BSS, Alcon Nederland BV, Gorinchem, Nederland) and stained two times with trypan blue 0.06% (Vision Blue, D.O.R.C. International, The Netherlands). A direct flow of BSS is used on top of the DMEK graft to open up the roll and to create a double roll. (Source: American Journal of Ophthalmology)
Source: American Journal of Ophthalmology - April 12, 2024 Category: Opthalmology Authors: Gergely Losonczy Tags: Illustrations & Insights Source Type: research
Increased Anti-Inflammatory Therapeutic Potential and Progenitor Marker Expression of Corneal Mesenchymal Stem Cells Cultured in an Optimized Propagation Medium
Cell Transplant. 2024 Jan-Dec;33:9636897241241992. doi: 10.1177/09636897241241992.ABSTRACTThere is a huge unmet need for new treatment modalities for ocular surface inflammatory disorders (OSIDs) such as dry eye disease and meibomian gland dysfunction. Mesenchymal stem cell therapies may hold the answer due to their potent immunomodulatory properties, low immunogenicity, and ability to modulate both the innate and adaptive immune response. MSC-like cells that can be isolated from the corneal stroma (C-MSCs) offer a potential new treatment strategy; however, an optimized culture medium needs to be developed to produce the i...
Source: Cell Transplantation - April 11, 2024 Category: Cytology Authors: Andrew Hopkinson Maria Notara Claus Cursiefen Laura E Sidney Source Type: research
Increased Anti-Inflammatory Therapeutic Potential and Progenitor Marker Expression of Corneal Mesenchymal Stem Cells Cultured in an Optimized Propagation Medium
Cell Transplant. 2024 Jan-Dec;33:9636897241241992. doi: 10.1177/09636897241241992.ABSTRACTThere is a huge unmet need for new treatment modalities for ocular surface inflammatory disorders (OSIDs) such as dry eye disease and meibomian gland dysfunction. Mesenchymal stem cell therapies may hold the answer due to their potent immunomodulatory properties, low immunogenicity, and ability to modulate both the innate and adaptive immune response. MSC-like cells that can be isolated from the corneal stroma (C-MSCs) offer a potential new treatment strategy; however, an optimized culture medium needs to be developed to produce the i...
Source: Cell Transplantation - April 11, 2024 Category: Cytology Authors: Andrew Hopkinson Maria Notara Claus Cursiefen Laura E Sidney Source Type: research
Increased Anti-Inflammatory Therapeutic Potential and Progenitor Marker Expression of Corneal Mesenchymal Stem Cells Cultured in an Optimized Propagation Medium
Cell Transplant. 2024 Jan-Dec;33:9636897241241992. doi: 10.1177/09636897241241992.ABSTRACTThere is a huge unmet need for new treatment modalities for ocular surface inflammatory disorders (OSIDs) such as dry eye disease and meibomian gland dysfunction. Mesenchymal stem cell therapies may hold the answer due to their potent immunomodulatory properties, low immunogenicity, and ability to modulate both the innate and adaptive immune response. MSC-like cells that can be isolated from the corneal stroma (C-MSCs) offer a potential new treatment strategy; however, an optimized culture medium needs to be developed to produce the i...
Source: Cell Transplantation - April 11, 2024 Category: Cytology Authors: Andrew Hopkinson Maria Notara Claus Cursiefen Laura E Sidney Source Type: research
Increased Anti-Inflammatory Therapeutic Potential and Progenitor Marker Expression of Corneal Mesenchymal Stem Cells Cultured in an Optimized Propagation Medium
Cell Transplant. 2024 Jan-Dec;33:9636897241241992. doi: 10.1177/09636897241241992.ABSTRACTThere is a huge unmet need for new treatment modalities for ocular surface inflammatory disorders (OSIDs) such as dry eye disease and meibomian gland dysfunction. Mesenchymal stem cell therapies may hold the answer due to their potent immunomodulatory properties, low immunogenicity, and ability to modulate both the innate and adaptive immune response. MSC-like cells that can be isolated from the corneal stroma (C-MSCs) offer a potential new treatment strategy; however, an optimized culture medium needs to be developed to produce the i...
Source: Cell Transplantation - April 11, 2024 Category: Cytology Authors: Andrew Hopkinson Maria Notara Claus Cursiefen Laura E Sidney Source Type: research
