"Keep on ROCKIn": Repurposed ROCK inhibitors to boost corneal endothelial regeneration
This study investigates the repurposing potential of additional ROCKi compounds. Through screening a self-assembled library of ROCKi on B4G12 corneal endothelial cells, we evaluated their dose-dependent effects on proliferation, migration, and toxicity using live-cell imaging. Nine ROCKi candidates significantly enhanced B4G12 proliferation compared to the basal growth rate. These candidates were further assessed for their potential to accelerate wound closure as another indicator for tissue regeneration capacity, with most demonstrating notable efficacy. To assess the potential impact of candidate ROCKi on key corneal end...
Source: Biomedicine and pharmacotherapy = Biomedecine and pharmacotherapie - March 21, 2024 Category: Drugs & Pharmacology Authors: Hendrik Vercammen Martin Ondra Jana Kotulova Edgar Cardenas De La Hoz Charissa Witters Katerina Jecmenova Maxim Le Compte Christophe Deben Sorcha N í Dhubhghaill Carina Koppen Mari án Hajdúch Bert Van den Bogerd Source Type: research
Validation of preloaded DMEK donor tissues: a laboratory-based study on endothelial cell viability and comparison of two F-mark inks
CONCLUSION: Preloaded DMEK donor tissues resulted in an acceptable ECL range after 1 and 5 days of storage and were deemed suitable for transplantation. Both F-mark inks are acceptable for prestamping preloaded DMEK tissues prior to surgical transplantation with comparable ECL and intensities.PMID:38513716 | DOI:10.1016/j.jcjo.2024.02.012 (Source: Canadian Journal of Ophthalmology)
Source: Canadian Journal of Ophthalmology - March 21, 2024 Category: Opthalmology Authors: Narisa Dhupar Sara AlShaker Jenny Chai Judy Yan Ness Little Christine Humphreys Clara C Chan Stephan Ong Tone Source Type: research
"Keep on ROCKIn": Repurposed ROCK inhibitors to boost corneal endothelial regeneration
This study investigates the repurposing potential of additional ROCKi compounds. Through screening a self-assembled library of ROCKi on B4G12 corneal endothelial cells, we evaluated their dose-dependent effects on proliferation, migration, and toxicity using live-cell imaging. Nine ROCKi candidates significantly enhanced B4G12 proliferation compared to the basal growth rate. These candidates were further assessed for their potential to accelerate wound closure as another indicator for tissue regeneration capacity, with most demonstrating notable efficacy. To assess the potential impact of candidate ROCKi on key corneal end...
Source: Biomedicine and pharmacotherapy = Biomedecine and pharmacotherapie - March 21, 2024 Category: Drugs & Pharmacology Authors: Hendrik Vercammen Martin Ondra Jana Kotulova Edgar Cardenas De La Hoz Charissa Witters Katerina Jecmenova Maxim Le Compte Christophe Deben Sorcha N í Dhubhghaill Carina Koppen Mari án Hajdúch Bert Van den Bogerd Source Type: research
Validation of preloaded DMEK donor tissues: a laboratory-based study on endothelial cell viability and comparison of two F-mark inks
CONCLUSION: Preloaded DMEK donor tissues resulted in an acceptable ECL range after 1 and 5 days of storage and were deemed suitable for transplantation. Both F-mark inks are acceptable for prestamping preloaded DMEK tissues prior to surgical transplantation with comparable ECL and intensities.PMID:38513716 | DOI:10.1016/j.jcjo.2024.02.012 (Source: Canadian Journal of Ophthalmology)
Source: Canadian Journal of Ophthalmology - March 21, 2024 Category: Opthalmology Authors: Narisa Dhupar Sara AlShaker Jenny Chai Judy Yan Ness Little Christine Humphreys Clara C Chan Stephan Ong Tone Source Type: research
Validation of preloaded DMEK donor tissues: a laboratory-based study on endothelial cell viability and comparison of two F-mark inks
CONCLUSION: Preloaded DMEK donor tissues resulted in an acceptable ECL range after 1 and 5 days of storage and were deemed suitable for transplantation. Both F-mark inks are acceptable for prestamping preloaded DMEK tissues prior to surgical transplantation with comparable ECL and intensities.PMID:38513716 | DOI:10.1016/j.jcjo.2024.02.012 (Source: Canadian Journal of Ophthalmology)
Source: Canadian Journal of Ophthalmology - March 21, 2024 Category: Opthalmology Authors: Narisa Dhupar Sara AlShaker Jenny Chai Judy Yan Ness Little Christine Humphreys Clara C Chan Stephan Ong Tone Source Type: research
Validation of preloaded DMEK donor tissues: a laboratory-based study on endothelial cell viability and comparison of two F-mark inks
CONCLUSION: Preloaded DMEK donor tissues resulted in an acceptable ECL range after 1 and 5 days of storage and were deemed suitable for transplantation. Both F-mark inks are acceptable for prestamping preloaded DMEK tissues prior to surgical transplantation with comparable ECL and intensities.PMID:38513716 | DOI:10.1016/j.jcjo.2024.02.012 (Source: Canadian Journal of Ophthalmology)
Source: Canadian Journal of Ophthalmology - March 21, 2024 Category: Opthalmology Authors: Narisa Dhupar Sara AlShaker Jenny Chai Judy Yan Ness Little Christine Humphreys Clara C Chan Stephan Ong Tone Source Type: research
Validation of preloaded DMEK donor tissues: a laboratory-based study on endothelial cell viability and comparison of two F-mark inks
CONCLUSION: Preloaded DMEK donor tissues resulted in an acceptable ECL range after 1 and 5 days of storage and were deemed suitable for transplantation. Both F-mark inks are acceptable for prestamping preloaded DMEK tissues prior to surgical transplantation with comparable ECL and intensities.PMID:38513716 | DOI:10.1016/j.jcjo.2024.02.012 (Source: Canadian Journal of Ophthalmology)
Source: Canadian Journal of Ophthalmology - March 21, 2024 Category: Opthalmology Authors: Narisa Dhupar Sara AlShaker Jenny Chai Judy Yan Ness Little Christine Humphreys Clara C Chan Stephan Ong Tone Source Type: research
Validation of preloaded DMEK donor tissues: a laboratory-based study on endothelial cell viability and comparison of two F-mark inks
CONCLUSION: Preloaded DMEK donor tissues resulted in an acceptable ECL range after 1 and 5 days of storage and were deemed suitable for transplantation. Both F-mark inks are acceptable for prestamping preloaded DMEK tissues prior to surgical transplantation with comparable ECL and intensities.PMID:38513716 | DOI:10.1016/j.jcjo.2024.02.012 (Source: Canadian Journal of Ophthalmology)
Source: Canadian Journal of Ophthalmology - March 21, 2024 Category: Opthalmology Authors: Narisa Dhupar Sara AlShaker Jenny Chai Judy Yan Ness Little Christine Humphreys Clara C Chan Stephan Ong Tone Source Type: research
Validation of preloaded DMEK donor tissues: a laboratory-based study on endothelial cell viability and comparison of two F-mark inks
CONCLUSION: Preloaded DMEK donor tissues resulted in an acceptable ECL range after 1 and 5 days of storage and were deemed suitable for transplantation. Both F-mark inks are acceptable for prestamping preloaded DMEK tissues prior to surgical transplantation with comparable ECL and intensities.PMID:38513716 | DOI:10.1016/j.jcjo.2024.02.012 (Source: Canadian Journal of Ophthalmology)
Source: Canadian Journal of Ophthalmology - March 21, 2024 Category: Opthalmology Authors: Narisa Dhupar Sara AlShaker Jenny Chai Judy Yan Ness Little Christine Humphreys Clara C Chan Stephan Ong Tone Source Type: research
Validation of preloaded DMEK donor tissues: a laboratory-based study on endothelial cell viability and comparison of two F-mark inks
CONCLUSION: Preloaded DMEK donor tissues resulted in an acceptable ECL range after 1 and 5 days of storage and were deemed suitable for transplantation. Both F-mark inks are acceptable for prestamping preloaded DMEK tissues prior to surgical transplantation with comparable ECL and intensities.PMID:38513716 | DOI:10.1016/j.jcjo.2024.02.012 (Source: Canadian Journal of Ophthalmology)
Source: Canadian Journal of Ophthalmology - March 21, 2024 Category: Opthalmology Authors: Narisa Dhupar Sara AlShaker Jenny Chai Judy Yan Ness Little Christine Humphreys Clara C Chan Stephan Ong Tone Source Type: research
Validation of preloaded DMEK donor tissues: a laboratory-based study on endothelial cell viability and comparison of two F-mark inks
CONCLUSION: Preloaded DMEK donor tissues resulted in an acceptable ECL range after 1 and 5 days of storage and were deemed suitable for transplantation. Both F-mark inks are acceptable for prestamping preloaded DMEK tissues prior to surgical transplantation with comparable ECL and intensities.PMID:38513716 | DOI:10.1016/j.jcjo.2024.02.012 (Source: Canadian Journal of Ophthalmology)
Source: Canadian Journal of Ophthalmology - March 21, 2024 Category: Opthalmology Authors: Narisa Dhupar Sara AlShaker Jenny Chai Judy Yan Ness Little Christine Humphreys Clara C Chan Stephan Ong Tone Source Type: research
Corneal Plug Keratoplasty for the Treatment of Small Corneal Perforation
This case report describes the performance of corneal plug keratoplasty in a male patient aged 30 years who presented with corneal perforation secondary to metal foreign body. (Source: JAMA Ophthalmology)
Source: JAMA Ophthalmology - March 21, 2024 Category: Opthalmology Source Type: research
PCR testing for herpesviruses in aqueous humor samples from patients with and without clinical corneal endothelial graft rejection
J Med Virol. 2024 Mar;96(3):e29538. doi: 10.1002/jmv.29538.ABSTRACTTo compare prevalence of positive PCR tests for herpesviruses between patients with and without a history of clinical corneal endothelial allograft rejection (AGR). Retrospective cross-sectional study with two-group comparison. A total of 307 aqueous humor (AH) samples from 235 Patients and 244 eyes who underwent penetrating keratoplasty or Descemet membrane endothelial keratoplasty or had a diagnostic AH aspiration due to clinical AGR between 2019 and 2023 were tested for DNA of herpes simplex virus (HSV), varicella-zoster virus (VZV), cytomegalovirus (CMV...
Source: Herpes - March 20, 2024 Category: Infectious Diseases Authors: Yaser Abu Dail Loay Daas Elias Flockerzi Cristian Munteanu Julian Kahlert Sigrun Smola Berthold Seitz Source Type: research
PCR testing for herpesviruses in aqueous humor samples from patients with and without clinical corneal endothelial graft rejection
J Med Virol. 2024 Mar;96(3):e29538. doi: 10.1002/jmv.29538.ABSTRACTTo compare prevalence of positive PCR tests for herpesviruses between patients with and without a history of clinical corneal endothelial allograft rejection (AGR). Retrospective cross-sectional study with two-group comparison. A total of 307 aqueous humor (AH) samples from 235 Patients and 244 eyes who underwent penetrating keratoplasty or Descemet membrane endothelial keratoplasty or had a diagnostic AH aspiration due to clinical AGR between 2019 and 2023 were tested for DNA of herpes simplex virus (HSV), varicella-zoster virus (VZV), cytomegalovirus (CMV...
Source: Herpes - March 20, 2024 Category: Infectious Diseases Authors: Yaser Abu Dail Loay Daas Elias Flockerzi Cristian Munteanu Julian Kahlert Sigrun Smola Berthold Seitz Source Type: research
At a glance
Characterisation of ectasia after penetrating keratoplasty in keratoconus eyes using anterior-segment optical coherence tomography (see page 506) Optical coherence tomography can be used to image corneal ectasia which occurs late following penetrating keratoplasty. Corneas with late post-keratoplasty ectasia have a deeper anterior chamber, steeper graft-host interface angle, and lower thickness at the interface Crystalline lens nuclear age prediction as a new biomarker of nucleus degeneration (see page 513) A deep learning algorithm was developed to predict nuclear age, which provides insight into the premature ageing of t...
Source: British Journal of Ophthalmology - March 20, 2024 Category: Opthalmology Authors: Larkin, F. Tags: Open access Highlights from this issue Source Type: research
