How to Plan and Carry Out a Simple Self-Experiment, a Single Person Trial of Khavinson Peptides for Thymic Regrowth

The objective here is to produce doses of the peptides dissolved in 0.5 ml of phosphate buffered saline in sealed vials, ready to be used with the injection system, with as little contamination as possible from the environment, and stored a freezer until it is ready to use. Depending on the size of the vial, it might be able to contain doses for multiple injections, but it is better to stick to one dose per vial. Peptides are sensitive to free-thaw cycles, so you want as few of those as possible. When ordering epitalon, thymogen, and vilon, they will arrive as lyophilized (freeze-dried) crystals or powder. Thymogen must be shipped on ice, as it is not very stable at room temperature. (a) Divide the lyophized peptides into 100mg amounts, and place into a freezer. (b) Every month pull out 100mg of each peptide, and dissolve all three 100mg amounts into the same 10ml of phosphate buffered saline, with the addition of five drops of DMSO to aid in solubility. Thymogen is a very light, fine powder, and 10ml is right on the edge of what is required to dissolve 100mg. You may see a few flecks remaining. (c) Split the 10ml solution into 20 vials of 0.5ml each. This is a lot of precision pipetting, so practice first! (d) Seal the vials and place them into the freezer, for use in injections that month. Keeping Things Sterile is Very Important Keeping hands, tools, vials, and surfaces clean and sterile is important: wash everything carefully and wipe down surfaces with a...
Source: Fight Aging! - Category: Research Authors: Tags: Self-Experimentation Source Type: blogs