Surface Engineering of < em > Escherichia coli < /em > to Display Its Phytase (AppA) and Functional Analysis of Enzyme Activities
This study explored a homologous protein production approach for displaying AppA on the cell surface of E. coli by engineering its outer membrane (OM) for extracellular expression. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis of total bacterial lysates and immunofluorescence microscopy of non-permeabilized cells revealed protein expression, whereas activity assays using whole cells or OM fractions indicated functional enzyme display, as evidenced by consistent hydrolytic rates on typical substrates (i.e., p-nitrophenyl phosphate and phytic acid). Furthermore, the in vitro results obtained using a simp...
Source: Current Issues in Molecular Biology - April 26, 2024 Category: Molecular Biology Authors: Patricia L A Mu ñoz-Muñoz Celina Ter án-Ramírez Rosa E Mares-Alejandre Ariana B M árquez-González Pablo A Madero-Ayala Samuel G Mel éndez-López Marco A Ramos-Ibarra Source Type: research
Clinical efficacy of sodium aescinate administration following endovenous laser ablation for varicose veins
CONCLUSIONS: SA, in combination with compression therapy, can relieve edema and alleviate pain in patients following EVLA for varicose veins.PMID:38670962 | DOI:10.1177/17085381241249288 (Source: Vascular)
Source: Vascular - April 26, 2024 Category: Surgery Authors: Xinrui Yang Jian Jin Siyi Huang Peng Qiu Ruihua Wang Kaichuang Ye Xinwu Lu Source Type: research
CLSI-based verification and < em > de novo < /em > establishment of reference intervals for common biochemical assays in Croatian newborns
CONCLUSIONS: The CALIPER reference intervals can be implemented into routine laboratory and clinical practice for the tested newborn population for most of the analyzed assays, while own reference intervals for potassium, magnesium and direct bilirubin have been determined.PMID:38665867 | PMC:PMC11042559 | DOI:10.11613/BM.2024.020705 (Source: Biochemia Medica)
Source: Biochemia Medica - April 26, 2024 Category: Biochemistry Authors: Iva Fri ščić Sonja Perkov Andrea Radeljak Jasminka Stipanovi ć-Kastelić Mirjana Mariana Kardum Paro Source Type: research
Mitochondrial (mt)DNA-cyclic GMP-AMP synthase (cGAS)-stimulator of interferon genes (STING) signaling promotes pyroptosis of macrophages via interferon regulatory factor (IRF)7/IRF3 activation to aggravate lung injury during severe acute pancreatitis
CONCLUSIONS: Negative regulation of any molecule in the mtDNA-cGAS-STING-IRF7/IRF3 pathway can affect the activation of NLRP3 inflammasomes, thereby reducing macrophage pyroptosis and improving SAP-ALI in mouse model.PMID:38671352 | PMC:PMC11055249 | DOI:10.1186/s11658-024-00575-9 (Source: Cellular and Molecular Biology Letters)
Source: Cellular and Molecular Biology Letters - April 26, 2024 Category: Biochemistry Authors: Yiqiu Peng Yuxi Yang Yingying Li Tingjuan Shi Ning Xu Ruixia Liu Yingyi Luan Yongming Yao Chenghong Yin Source Type: research
Tropomyosin 2 Regulates Tumor Cell Proliferation, Immune Suppression, and Activation of the JNK Signaling Pathway in Colitis-Associated Cancer (CAC)
CONCLUSIONS: Our results demonstrated that TPM2 knockdown suppressed the proliferation of CAC IECs, enhanced immune suppression on CAC IECs, and inhibited the JNK signaling pathway within the framework of CAC. These findings suggest TPM2 can serve as a potential therapeutic target for CAC treatment.PMID:38665026 | DOI:10.24976/Discov.Med.202436183.73 (Source: Discovery Medicine)
Source: Discovery Medicine - April 26, 2024 Category: Research Authors: Aixi Sun Jian Ge Kaixin Ding Zhiyang Gao Yun Zhang Source Type: research
Surface Engineering of < em > Escherichia coli < /em > to Display Its Phytase (AppA) and Functional Analysis of Enzyme Activities
This study explored a homologous protein production approach for displaying AppA on the cell surface of E. coli by engineering its outer membrane (OM) for extracellular expression. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis of total bacterial lysates and immunofluorescence microscopy of non-permeabilized cells revealed protein expression, whereas activity assays using whole cells or OM fractions indicated functional enzyme display, as evidenced by consistent hydrolytic rates on typical substrates (i.e., p-nitrophenyl phosphate and phytic acid). Furthermore, the in vitro results obtained using a simp...
Source: Current Issues in Molecular Biology - April 26, 2024 Category: Molecular Biology Authors: Patricia L A Mu ñoz-Muñoz Celina Ter án-Ramírez Rosa E Mares-Alejandre Ariana B M árquez-González Pablo A Madero-Ayala Samuel G Mel éndez-López Marco A Ramos-Ibarra Source Type: research
Ultralong-life and high-capacity magnesium/sodium hybrid-ion battery using a ternary CoSe/NiSe < sub > 2 < /sub > /CuSe < sub > 2 < /sub > cathode and dual-ion electrolyte
Chem Commun (Camb). 2024 Apr 26. doi: 10.1039/d4cc00623b. Online ahead of print.ABSTRACTAn ultrahigh-performance magnesium/sodium hybrid-ion battery (MNHB) is developed using ternary CoSe/NiSe2/CuSe2 (CNCS) "micro-flowers" as cathode materials, working with a coordinative [Mg2Cl2][AlCl4]2 and bis(trifluoroethylsulfonyl)imide anionic sodium salt in triglyme electrolyte. After 2000 cycles at 2.0 A g-1, the MNHB shows a stable capacity of 115.5 mA h g-1 and a high Coulombic efficiency exceeding 99.8%. The battery shows very rapid charging, and good stability in extreme environments, providing new opportunities to develop othe...
Source: Chemical Communications - April 26, 2024 Category: Chemistry Authors: Ting Zhou Yajun Zhu Yun Shen Hui Qiu Tianli Han Jinjin Li Jinyun Liu Source Type: research
Clinical efficacy of sodium aescinate administration following endovenous laser ablation for varicose veins
CONCLUSIONS: SA, in combination with compression therapy, can relieve edema and alleviate pain in patients following EVLA for varicose veins.PMID:38670962 | DOI:10.1177/17085381241249288 (Source: Vascular)
Source: Vascular - April 26, 2024 Category: Surgery Authors: Xinrui Yang Jian Jin Siyi Huang Peng Qiu Ruihua Wang Kaichuang Ye Xinwu Lu Source Type: research
CLSI-based verification and < em > de novo < /em > establishment of reference intervals for common biochemical assays in Croatian newborns
CONCLUSIONS: The CALIPER reference intervals can be implemented into routine laboratory and clinical practice for the tested newborn population for most of the analyzed assays, while own reference intervals for potassium, magnesium and direct bilirubin have been determined.PMID:38665867 | PMC:PMC11042559 | DOI:10.11613/BM.2024.020705 (Source: Biochemia Medica)
Source: Biochemia Medica - April 26, 2024 Category: Biochemistry Authors: Iva Fri ščić Sonja Perkov Andrea Radeljak Jasminka Stipanovi ć-Kastelić Mirjana Mariana Kardum Paro Source Type: research
Tropomyosin 2 Regulates Tumor Cell Proliferation, Immune Suppression, and Activation of the JNK Signaling Pathway in Colitis-Associated Cancer (CAC)
CONCLUSIONS: Our results demonstrated that TPM2 knockdown suppressed the proliferation of CAC IECs, enhanced immune suppression on CAC IECs, and inhibited the JNK signaling pathway within the framework of CAC. These findings suggest TPM2 can serve as a potential therapeutic target for CAC treatment.PMID:38665026 | DOI:10.24976/Discov.Med.202436183.73 (Source: Discovery Medicine)
Source: Discovery Medicine - April 26, 2024 Category: Research Authors: Aixi Sun Jian Ge Kaixin Ding Zhiyang Gao Yun Zhang Source Type: research
Mitochondrial (mt)DNA-cyclic GMP-AMP synthase (cGAS)-stimulator of interferon genes (STING) signaling promotes pyroptosis of macrophages via interferon regulatory factor (IRF)7/IRF3 activation to aggravate lung injury during severe acute pancreatitis
CONCLUSIONS: Negative regulation of any molecule in the mtDNA-cGAS-STING-IRF7/IRF3 pathway can affect the activation of NLRP3 inflammasomes, thereby reducing macrophage pyroptosis and improving SAP-ALI in mouse model.PMID:38671352 | PMC:PMC11055249 | DOI:10.1186/s11658-024-00575-9 (Source: Cellular and Molecular Biology Letters)
Source: Cellular and Molecular Biology Letters - April 26, 2024 Category: Biochemistry Authors: Yiqiu Peng Yuxi Yang Yingying Li Tingjuan Shi Ning Xu Ruixia Liu Yingyi Luan Yongming Yao Chenghong Yin Source Type: research
Photo trapping voltage-gated cardiac sodium channels: inferring local motions at the 'inactivation gate'
Biophys J. 2024 Apr 24:S0006-3495(24)00279-0. doi: 10.1016/j.bpj.2024.04.017. Online ahead of print.ABSTRACTRapid and effectual inactivation in voltage-gated sodium channels is required for canonical action-potential firing. This "fast" inactivation arises from swift and reversible protein conformational changes that utilize transmembrane segments and the cytoplasmic linker between channel domains III and IV. Until recently, fast inactivation had been accepted to rely on a "ball and chain" mechanism whereby a hydrophobic triplet of DIII-IV amino acids (IFM) impairs conductance by binding to a site in central pore of the ch...
Source: Biophysical Journal - April 26, 2024 Category: Physics Authors: Samuel J Goodchild Christopher A Ahern Source Type: research
New Insights into the Substrate Recognition and Transport Mechanism of the Human Sodium-Taurocholate Cotransporter
Biophys J. 2024 Apr 24:S0006-3495(24)00286-8. doi: 10.1016/j.bpj.2024.04.024. Online ahead of print.NO ABSTRACTPMID:38664964 | DOI:10.1016/j.bpj.2024.04.024 (Source: Biophysical Journal)
Source: Biophysical Journal - April 26, 2024 Category: Physics Authors: Jana Shen Source Type: research
Surface Engineering of < em > Escherichia coli < /em > to Display Its Phytase (AppA) and Functional Analysis of Enzyme Activities
This study explored a homologous protein production approach for displaying AppA on the cell surface of E. coli by engineering its outer membrane (OM) for extracellular expression. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis of total bacterial lysates and immunofluorescence microscopy of non-permeabilized cells revealed protein expression, whereas activity assays using whole cells or OM fractions indicated functional enzyme display, as evidenced by consistent hydrolytic rates on typical substrates (i.e., p-nitrophenyl phosphate and phytic acid). Furthermore, the in vitro results obtained using a simp...
Source: Current Issues in Molecular Biology - April 26, 2024 Category: Molecular Biology Authors: Patricia L A Mu ñoz-Muñoz Celina Ter án-Ramírez Rosa E Mares-Alejandre Ariana B M árquez-González Pablo A Madero-Ayala Samuel G Mel éndez-López Marco A Ramos-Ibarra Source Type: research
Ultralong-life and high-capacity magnesium/sodium hybrid-ion battery using a ternary CoSe/NiSe < sub > 2 < /sub > /CuSe < sub > 2 < /sub > cathode and dual-ion electrolyte
Chem Commun (Camb). 2024 Apr 26. doi: 10.1039/d4cc00623b. Online ahead of print.ABSTRACTAn ultrahigh-performance magnesium/sodium hybrid-ion battery (MNHB) is developed using ternary CoSe/NiSe2/CuSe2 (CNCS) "micro-flowers" as cathode materials, working with a coordinative [Mg2Cl2][AlCl4]2 and bis(trifluoroethylsulfonyl)imide anionic sodium salt in triglyme electrolyte. After 2000 cycles at 2.0 A g-1, the MNHB shows a stable capacity of 115.5 mA h g-1 and a high Coulombic efficiency exceeding 99.8%. The battery shows very rapid charging, and good stability in extreme environments, providing new opportunities to develop othe...
Source: Chemical Communications - April 26, 2024 Category: Chemistry Authors: Ting Zhou Yajun Zhu Yun Shen Hui Qiu Tianli Han Jinjin Li Jinyun Liu Source Type: research
