Filtered By:
Specialty: Opthalmology

This page shows you your search results in order of date. This is page number 5.

Order by Relevance | Date

Total 178 results found since Jan 2013.

GSDMD, an executor of pyroptosis, is involved in IL-1 β secretion in Aspergillus fumigatus keratitis.
GSDMD, an executor of pyroptosis, is involved in IL-1β secretion in Aspergillus fumigatus keratitis. Exp Eye Res. 2020 Dec 03;:108375 Authors: Zhao W, Yang H, Lyu L, Zhang J, Xu Q, Jiang N, Liu G, Wang L, Yan H, Che C Abstract The protein GSDMD is an important performer of pyroptosis and a universal substrate for the inflammatory caspase. However, the role and regulatory mechanism of GSDMD in Aspergillus fumigatus keratitis is remains unknown. Here we detected GSDMD protein in the cornea of normal and fungal-infected C57BL/6 mice. Human corneal epithelial cell (HCECs) were preincubated with a hydroch...
Source: Experimental Eye Research - December 3, 2020 Category: Opthalmology Authors: Zhao W, Yang H, Lyu L, Zhang J, Xu Q, Jiang N, Liu G, Wang L, Yan H, Che C Tags: Exp Eye Res Source Type: research

The cGAS-STING signaling pathway contributes to the inflammatory response and autophagy in Aspergillus fumigatus keratitis.
In this study, we showed that the cGAS-STING signaling pathway was activated in human corneal epithelial cells (HCECs) and in mouse corneas infected with A. fumigatus. Knockdown of cGAS reduced A. fumigatus-induced production of pro-inflammatory cytokines, including TNF-α, IL-1β, IL-6, and IFN-β. However, reconstruction of cGAS activity restored the inflammatory response in HCECs infected with A. fumigatus. A specific cGAS inhibitor, RU.521, could also significantly inhibit A. fumigatus-induced inflammatory cytokine expression. In addition, we found that cGAS was indispensable for the autophagy flux evoked by A. fumigat...
Source: Experimental Eye Research - November 20, 2020 Category: Opthalmology Authors: Han F, Guo H, Wang L, Zhang Y, Sun L, Dai C, Wu X Tags: Exp Eye Res Source Type: research

RhoA activation decreases phagocytosis of trabecular meshwork cells.
CONCLUSIONS: These results suggest that the RhoA signal pathway regulates the phagocytic activity of TM cells. PMID: 32847411 [PubMed - as supplied by publisher]
Source: Current Eye Research - August 25, 2020 Category: Opthalmology Authors: Fujimoto T, Sato-Ohira S, Tanihara H, Inoue T Tags: Curr Eye Res Source Type: research

In Vitro Screening and Transfection Concentration Optimization of Cynomolgus Monkey I κBα-siRNA.
Conclusion: It proves that the siRNA targeting the GCACTTAGCCTCTATCCAT of IκBα gene is the valid sequence to suppress cynomolgus monkey IκBα expression of CM cells and TM cells by RNAi. 10 nM is the optimal transfection concentration. PMID: 32377413 [PubMed]
Source: Journal of Ophthalmology - May 8, 2020 Category: Opthalmology Tags: J Ophthalmol Source Type: research

RvE1 uses the LTB4 receptor BLT1 to increase Ca2+i and stimulate mucin secretion in cultured rat and human conjunctival goblet cells.
CONCLUSION: RvE1, in addition to the ChemR23 receptor, uses the BLT1 receptor to increase [Ca2+]i and stimulate secretion in both rat and human cultured conjunctival goblet cells. PMID: 32361084 [PubMed - as supplied by publisher]
Source: The Ocular Surface - April 28, 2020 Category: Opthalmology Authors: Yang M, Lippestad M, Hodges RR, Fjærvoll HK, Fjærvoll KA, Bair JA, Utheim TP, Serhan CN, Dartt DA Tags: Ocul Surf Source Type: research

Inhibition of acetylcholinesterase attenuated retinal inflammation via suppressing NF- κB activation.
This study was aimed to dissect the role of ACHE in retinal inflammation, and its mechanism of action. Retinal inflammation was induced by intravitreal injection of tumor necrosis factor-α (TNF-α) in heterozygous ACHE knockout mice (ACHE+/-) and wild type mice (ACHE+/+). Donepezil, a well-known ACHE inhibitor, was administrated by daily gavage. Expression of ACHE and intercellular adherent molecule-1 (ICAM-1), infiltration of CD11b+ inflammatory cells, retinal leukostasis and vascular leakage was determined in both ACHE ± and ACHE+/+ mice. ARPE-19 cells, a human retinal pigment epithelial cell line, were cultured for ...
Source: Experimental Eye Research - March 13, 2020 Category: Opthalmology Authors: Li J, Chen Y, Zhang X, Ye S, Yi J, Chen Q, Liu Q Tags: Exp Eye Res Source Type: research

Silencing of p53 reduces cell migration in human Tenon's fibroblasts induced by TGF- β
Conclusionsp53 inhibited expression of TGF- β, suppressed HTFs migration and inhibited HTFs growth, by reducing miR-29b expression and interacting with miR29b gene in HTFs.
Source: International Ophthalmology - March 1, 2020 Category: Opthalmology Source Type: research

Receptor potential channels TRPC1/TRPC6 regulate lamina cribrosa cell extracellular matrix gene transcription and proliferation.
In conclusion, TRPC1/C6 expression is enhanced in glaucoma LC cells. These channels may contribute to oxidative stress-induced ECM gene transcription and cell proliferation in normal and glaucoma LC cells through Ca2+-NFATc3 signaling pathway mechanism. TRPC1 and TRPC6 channels could be important therapeutic targets to prevent ECM remodeling and fibrosis development in glaucoma optic neuropathy. PMID: 32088241 [PubMed - as supplied by publisher]
Source: Experimental Eye Research - February 19, 2020 Category: Opthalmology Authors: Irnaten M, O'Malley G, Clark AF, O'Brien CJ Tags: Exp Eye Res Source Type: research

Clinical Role of Epigenetics and Network Analysis in Eye Diseases: A Translational Science Review.
Authors: Lanza M, Benincasa G, Costa D, Napoli C Abstract Network medicine is a molecular-bioinformatic approach analyzing gene-gene interactions that can perturb the human interactome. This review focuses on epigenetic changes involved in several ocular diseases, such as DNA methylation, histone and nonhistone post-translational modifications, and noncoding RNA regulators. Although changes in aberrant DNA methylation play a major role in the pathogenesis of most ocular diseases, histone modifications are seldom investigated. Hypermethylation in TGM-2 and hypomethylation in MMP-2/CD24 promoter genes may play a cruc...
Source: Journal of Ophthalmology - January 25, 2020 Category: Opthalmology Tags: J Ophthalmol Source Type: research

APX-115A, a pan-NADPH oxidase inhibitor, induces caspase-dependent cell death by suppressing NOX4-ROS signaling in EBV-infected retinal epithelial cells.
Conclusion Taken together, these our results suggest that APX-115A could be a therapeutic agent for treating EBV-infected retinal cells or diseases by inhibiting LMP1-NOX4-ROS signaling. PMID: 31951764 [PubMed - as supplied by publisher]
Source: Current Eye Research - January 16, 2020 Category: Opthalmology Authors: Hong SW, Noh MH, Kim YS, Jin DH, Moon SH, Yang JW, Hur DY Tags: Curr Eye Res Source Type: research

Repression of microRNA-21 inhibits retinal vascular endothelial cell growth and angiogenesis via PTEN dependent-PI3K/Akt/VEGF signaling pathway in diabetic retinopathy.
This study is performed to explore the effects of microRNA-21 (miR-21) on retinal vascular endothelial cell (RVEC) viability and angiogenesis in rats with DR via the phosphatidylinositiol 3-kinase/protein kinase B (PI3K/Akt)/vascular endothelial growth factor (VEGF) signaling pathway by binding to phosphatase and tensin homolog (PTEN). Sprague Dawley (SD) rats were used for establishment of DR models. Target relationship between miR-21 and PTEN was assessed by bioinformatics prediction in combination with dual-luciferase reporter gene assay. Identification of expression of miR-21, PTEN and PI3K/Akt/VEGF signaling pathway-r...
Source: Experimental Eye Research - November 20, 2019 Category: Opthalmology Authors: Lu JM, Zhang ZZ, Ma X, Fang SF, Qin XH Tags: Exp Eye Res Source Type: research

Investigating impacts of surface charge on intraocular distribution of intravitreal lipid nanoparticles.
Abstract In this paper, the effect of surface charge of intravitreal lipid nanoparticles (LNPs) on their intraocular accumulation and distribution was quantitatively and systematically studied. The retinal distribution of LNPs delivering model drug (siRNA) with optimal surface charge was visualized as well. LNPs and siRNA-loaded LNPs (siLNPs) were prepared with ethanol-injection method. C57BL/6 Mice with the age of 6-8 weeks were used for the animal experiments. Quantitative accumulation in the eye and respective intraocular distribution of the intravitreally injected LNPs with different surface charge were examin...
Source: Experimental Eye Research - June 21, 2019 Category: Opthalmology Authors: Huang X, Chau Y Tags: Exp Eye Res Source Type: research

Cholesterol modification of SDF-1-specific siRNA enables therapeutic targeting of angiogenesis through Akt pathway inhibition.
Abstract Neovascularization during ocular tissue repair can cause severe visual loss in the optical axis and is therefore an issue of considerable concern to ophthalmologists. Here, we introduced a cholesterol-modified siRNA delivery system targeting stromal cell-derived factor 1 (SDF-1) to treat ocular angiogenesis in vivo. SDF-1 expression was analyzed in rat endothelial progenitor cells (EPCs) and bone marrow mesenchymal stem cells (BMSCs) using quantitative PCR (qPCR). Migration ability of BMSC and HUVEC were assessed through transwell assay. The proliferation effect of chol-siSDF1 on HUVEC was measured by col...
Source: Experimental Eye Research - March 17, 2019 Category: Opthalmology Authors: Chen J, Li F, Xu Y, Zhang W, Hu Y, Fu Y, Xu W, Ge S, Fan X, Lu L Tags: Exp Eye Res Source Type: research

Interactions of thymic stromal lymphopoietin with TLR2 and TLR4 regulate anti-fungal innate immunity in Aspergillus fumigatus-induced corneal infection.
Abstract Thymic stromal lymphopoietin (TSLP) is an interleukin 7 (IL-7)-like four helix bundle cytokine that plays diverse roles in the regulation of immune responses. In fungal infection, pattern recognition receptors (PRRs), including the cell surface Toll-like receptors (TLRs) and cytoplasmic NOD-like receptors, recognize pathogen-associated molecular patterns to initiate downstream signal cascades to active immune responses. Our previous studies reported that, in vitro human cornea epithelium cells represented a novel target of TSLP and that TSLP/TSLPR/STAT5 signaling played an important role in the response t...
Source: Experimental Eye Research - March 6, 2019 Category: Opthalmology Authors: Dai C, Wu J, Chen C, Wu X Tags: Exp Eye Res Source Type: research

The Effect of Rebamipide Ophthalmic Solution on Cytokine and Mucin Secretion in Culture of Conjunctival Epithelial Cells From the Cu, Zn-Superoxide Dismutase-1 (SOD-1) Knock-Down Mouse
Objectives: To evaluate the in vitro effects of 1-mM rebamipide ophthalmic solution on the expression of inflammatory cytokines and MUC5AC in Cu, Zn-superoxide dismutase-1 (SOD-1) knock-down conjunctival epithelium. Methods: Conjunctival epithelium from C57BL/6 wild-type mice was cultured and treated with rebamipide ophthalmic solution. Using cytometric bead array, we examined the levels of interleukin-(IL)-6, IL-10, IL-17, monocyte chemoattractant protein-1, interferon-γ (INF-γ), tumor necrosis factor, and IL-12p70 in the culture supernatants. The culture supernatants were obtained from the culture medium of nontre...
Source: Eye and Contact Lens: Science and Clinical Practice - February 26, 2019 Category: Opthalmology Tags: Article Source Type: research