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Specialty: Opthalmology
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Total 178 results found since Jan 2013.
Purinergic 2X 4 (P2X4), but not P2X7, receptors increase cytosolic Ca < sup > 2+ < /sup > and stimulate mucin secretion in rat conjunctival goblet cells to maintain ocular surface health
Exp Eye Res. 2023 Aug 12:109614. doi: 10.1016/j.exer.2023.109614. Online ahead of print.ABSTRACTIonotropic purinergic receptors (P2XRs) are activated by ATP and ATP analogs. ATP can be released through ATP-permeable channels such as the pannexin hemichannels. Upon activation, the P2XRs become permeable to Ca2+, a potent stimulator of mucin secretion in conjunctival goblet cells (CGCs). The purpose of this study was to investigate the presence and function of P2XRs in CGCs. We also examined the presence of pannexin hemichannels. Rat first passage CGCs were stained with the goblet cell marker anti-cytokeratin 7 antibody and ...
Source: Experimental Eye Research - August 14, 2023 Category: Opthalmology Authors: Haakon K Fj ærvoll Ketil A Fj ærvoll Menglu Yang Jeffrey Bair Tor P Utheim Darlene A Dartt Source Type: research
Heparanase-mediated histone 3 acetylation regulates VEGF gene transcription in the hyperglycemia and hypoxia human retinal endothelial cells
Exp Eye Res. 2023 Jun 3:109519. doi: 10.1016/j.exer.2023.109519. Online ahead of print.ABSTRACTHeparanase (HPA) is believed that might mediate histone 3 lysine 9 acetylation (H3K9ac) to regulate vascular endothelial growth factor (VEGF) gene expressions in the hyperglycemia and hypoxia human retinal endothelial cells (HRECs). Cultured human retinal endothelial cells (HRECs) in hyperglycemia, hypoxia, siRNA, and normal medium, respectively. Distributions of H3K9ac and HPA in HRECs were analyzed by immunofluorescence. Western blot and real-time PCR were respectively used to evaluate the expression of HPA, H3K9ac, and VEGF. T...
Source: Experimental Eye Research - June 5, 2023 Category: Opthalmology Authors: Xuan Leng Jingwei Wang Xin Song Jie Hu Lin Lu Source Type: research
Inhibition of AIF-1 alleviates laser-induced macular neovascularization by inhibiting endothelial cell proliferation via restrained p44/42 MAPK signaling pathway
This study aimed to understand how AIF-1 effects the proliferation of CECs and the subsequent progression of MNV. To study this, a mouse MNV model was established through laser injury, and the AIF-1 expression levels were then measured using western blot and immunohistochemistry. AIF-1 siRNA was intravitreally injected to silence AIF-1 gene expression. Western blot and choroidal flat mount were performed to measure the progression of MNV and proliferation of the CECs. These results showed that the protein expression of AIF-1 was significantly elevated in the laser-induced mouse MNV model, and the expression trend was consi...
Source: Experimental Eye Research - April 20, 2023 Category: Opthalmology Authors: Qinghua Li Liting Hu Guibo Liu Xiaoni Yin Ying Li Xiangyang Wei Ning Duan Xiaoran Zhao Qingyun Gong Zhaodong Du Source Type: research
Glabridin Inhibits < em > Aspergillus fumigatus < /em > Growth and Alleviate Inflammation Mediated by Dectin-2 and NLRP3 Inflammasome
CONCLUSION: Glabridin plays an anti-inflammatory role in A. fumigatus challenge via suppression of the Dectin-2 and NLRP3 inflammasome, and plays an anti-fungal role through delaying germination and changing the hyphal integrity.KEY MESSAGESGlabridin plays an anti-inflammatory role in A. fumigatus infection of RAW264.7 cells in a concentration-dependent manner and through Dectin-2 mediation.Glabridin decreases fungal distribution and inflammation in mouse A. fumigatus keratitis.Glabridin inhibits A. fumigatus growth by delaying germination and disrupting cellular structure in vitro.PMID:36785524 | DOI:10.1080/02713683.2022.2164779
Source: Current Eye Research - February 14, 2023 Category: Opthalmology Authors: Lu Zhan Xue Tian Jing Lin Xudong Peng Guiqiu Zhao Source Type: research
A20 functions as a negative regulator of the lipopolysaccharide-induced inflammation in corneal epithelial cells
Exp Eye Res. 2023 Jan 27:109392. doi: 10.1016/j.exer.2023.109392. Online ahead of print.ABSTRACTA20, also called TNFAIP3, is a crucial regulator of inflammation in various diseases but has not evidenced its function in the cornea. We aimed to evaluate the existence and the functions of A20 in human corneal epithelial (HCE-T) cells. After being treated with lipopolysaccharide (LPS) in different concentrations or at separate times, cells were collected to analyze A20 expressions. We then constructed the A20 knockdown system by siRNA and the A20 overexpressing system by lentivirus transduction. Systems were further exposed to...
Source: Experimental Eye Research - January 30, 2023 Category: Opthalmology Authors: Yubin Yu Kunke Li Ran Xue Sihao Liu Xiuping Liu Kaili Wu Source Type: research
Inhibition of diabetes-induced Drp1 deSUMOylation prevents retinal vascular lesions associated with diabetic retinopathy
Exp Eye Res. 2022 Nov 23:109334. doi: 10.1016/j.exer.2022.109334. Online ahead of print.ABSTRACTRetinal microvascular endothelial cell (RMEC) injury plays an important role in the pathophysiology diabetic retinopathy (DR). The GTPase dynamin-related protein 1 (Drp1), crucial to mitochondrial dynamics, has been implicated in hyperglycaemia-induced microvascular damage. Moreover, Drp1 can be deSUMOylated by the enzyme sentrin/SUMO-specific protease 3 (SENP3). Whether SENP3/deSUMOylated Drp1 can aggravate DR is unclear. Therefore, we designed this experiment to investigate the role of SENP3/desumoylated Drp1 in DR in vitro an...
Source: Experimental Eye Research - November 26, 2022 Category: Opthalmology Authors: Min Chen Qianhui Zhang Shun Wang Feng Zheng Source Type: research
