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Specialty: Opthalmology

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Total 178 results found since Jan 2013.

In Vitro Screening and Transfection Concentration Optimization of Cynomolgus Monkey I κBα-siRNA.
Conclusion: It proves that the siRNA targeting the GCACTTAGCCTCTATCCAT of IκBα gene is the valid sequence to suppress cynomolgus monkey IκBα expression of CM cells and TM cells by RNAi. 10 nM is the optimal transfection concentration. PMID: 32377413 [PubMed]
Source: Journal of Ophthalmology - May 8, 2020 Category: Opthalmology Tags: J Ophthalmol Source Type: research

Decrease of Murine Cytomegalovirus-Induced Retinitis by Intravenous Delivery of Immediate Early Protein-3-Specific siRNA Immunology and Microbiology
Conclusions. Systemic administration of IE-3–specific siRNA could alleviate MCMV retinitis by inhibiting virus replication and subsequent death of uninfected retinal cells.
Source: Investigative Ophthalmology - July 8, 2014 Category: Opthalmology Authors: Marshall, B., Mo, J., Covar, J., Atherton, S. S., Zhang, M. Tags: Immunology and Microbiology Source Type: research

CD25 siRNA induces Treg/Th1 cytokine expression in rat corneal transplantation models.
In conclusion, CD25 siRNA gene therapy played a protective role in corneal graft rejection via up-regulation of Treg cytokine expression and down-regulation of Th1 cytokine expression. PMID: 27567557 [PubMed - as supplied by publisher]
Source: Experimental Eye Research - August 23, 2016 Category: Opthalmology Authors: Qin Q, Luo D, Shi Y, Zhao Q, Chen Y, Wu J, Zhao M Tags: Exp Eye Res Source Type: research

Expression of retinoic acid signaling components ADH7 and ALDH1A1 is reduced in aniridia limbal epithelial cells and a siRNA primary cell based aniridia model.
Abstract PAX6-related Aniridia is a sight-threatening disease involving progression of secondary glaucoma and aniridia related keratopathy (ARK). Change or loss of limbal epithelial progenitors causes epithelial surface defects. We analyzed the effect of PAX6 on mRNA expression changes with a two-step approach, as follows. First, we sequenced mRNA from limbal epithelial cells isolated from controls and aniridia patients. Second, we confirmed the bioinformatics and literature-based result list for a small interfering RNA (siRNA)-based primary aniridia cell model (PAX6 knockdown). With this approach, we expected tha...
Source: Experimental Eye Research - October 4, 2018 Category: Opthalmology Authors: Latta L, Nordström K, Stachon T, Langenbucher A, Fries FN, Szentmáry N, Seitz B, Käsmann-Kellner B Tags: Exp Eye Res Source Type: research

Allele-Specific siRNA Silencing for the Common Keratin 12 Founder Mutation in Meesmann Epithelial Corneal Dystrophy Cornea
Conclusions. A potent allele-specific siRNA against the K12 Arg135Thr mutation was identified. In combination with efficient eyedrop formulation delivery, this would represent a personalized medicine approach, aimed at preventing the pathology associated with MECD and other ocular surface pathologies with dominant-negative or gain-of-function pathomechanisms.
Source: Investigative Ophthalmology - January 17, 2013 Category: Opthalmology Authors: Allen, E. H. A., Atkinson, S. D., Liao, H., Moore, J. E., Leslie Pedrioli, D. M., Smith, F. J. D., McLean, W. H. I., Moore, C. B. T. Tags: Cornea Source Type: research

siRNA Silencing of the Mutant Keratin 12 Allele in Corneal Limbal Epithelial Cells Grown From Patients With Meesmann's Epithelial Corneal Dystrophy Cornea
Conclusions. Combined with an effective delivery vehicle this siRNA approach represents a viable treatment option for prevention of the MECD pathology observed in K12-Leu132Pro heterozygous individuals.
Source: Investigative Ophthalmology - May 29, 2014 Category: Opthalmology Authors: Courtney, D. G., Atkinson, S. D., Allen, E. H. A., Moore, J. E., Walsh, C. P., Pedrioli, D. M. L., MacEwen, C. J., Pellegrini, G., Maurizi, E., Serafini, C., Fantacci, M., Liao, H., Irvine, A. D., McLean, W. H. I., Moore, C. B. T. Tags: Cornea Source Type: research

RTP801 Gene Expression Is Differentially Upregulated in Retinopathy and Is Silenced by PF-04523655, a 19-Mer siRNA Directed Against RTP801 Retina
Conclusions. In rodent retinopathy models, administration of the siRNA, PF-04523655, reduced RTP801 expression in the retina, consistent with the RNA-induced silencing complex (RISC) mechanism of action. The pharmacodynamic profile from the animal models could be useful to elucidate dose and exposure dependency of RTP801 expression inhibition by siRNA.
Source: Investigative Ophthalmology - March 4, 2014 Category: Opthalmology Authors: Rittenhouse, K. D., Johnson, T. R., Vicini, P., Hirakawa, B., Kalabat, D., Yang, A. H., Huang, W., Basile, A. S. Tags: Retina Source Type: research

Cholesterol modification of SDF-1-specific siRNA enables therapeutic targeting of angiogenesis through Akt pathway inhibition.
Abstract Neovascularization during ocular tissue repair can cause severe visual loss in the optical axis and is therefore an issue of considerable concern to ophthalmologists. Here, we introduced a cholesterol-modified siRNA delivery system targeting stromal cell-derived factor 1 (SDF-1) to treat ocular angiogenesis in vivo. SDF-1 expression was analyzed in rat endothelial progenitor cells (EPCs) and bone marrow mesenchymal stem cells (BMSCs) using quantitative PCR (qPCR). Migration ability of BMSC and HUVEC were assessed through transwell assay. The proliferation effect of chol-siSDF1 on HUVEC was measured by col...
Source: Experimental Eye Research - March 17, 2019 Category: Opthalmology Authors: Chen J, Li F, Xu Y, Zhang W, Hu Y, Fu Y, Xu W, Ge S, Fan X, Lu L Tags: Exp Eye Res Source Type: research

Therapeutic potential of topical administration of siRNAs against HIF-1 α for corneal neovascularization
In conclusion, the topical application of siRNA towards HIF-1α seems to be an effective and reliable method to stop neovascularization.PMID:35367249 | DOI:10.1016/j.exer.2022.109036
Source: Experimental Eye Research - April 3, 2022 Category: Opthalmology Authors: Assumpta Peral Jes ús Mateo Carmen O Dom ínguez-Godínez Gonzalo Carracedo Jose Antonio G ómez Almudena Crooke Jes ús Pintor Source Type: research

In vitro evaluation of anti HSV ‐1 siRNAs and in vivo evaluation of electroporation to transfect siRNAs on murine cornea
ConclusionsThese results demonstrate that siRNA directed against HSV‐1 DNA polymerase efficiently inhibits HSV‐1 replication, suggesting that siRNA based antiviral strategy may be a potential therapeutic alternative to treat HSK. Besides, intracorneal penetration may be facilitated by electroporation.
Source: Acta Ophthalmologica - September 13, 2016 Category: Opthalmology Authors: A. Rousseau, V. Escriou, P. Roy, N. Poccardi, J. Takissian, P. Bigey, M. Labetoulle Tags: Abstracts from the 2016 European Association for Vision and Eye Research Conference Source Type: research

Migration of Retinal Pigment Epithelium Cells Is Regulated by Protein Kinase C{alpha} In Vitro Retinal Cell Biology
Conclusions. Retinal pigment epithelium cell migration is enhanced by a PKCα agonist and suppressed by a PKCα antagonist. The results suggest that a PKCα-mediated signal transduction pathway plays a crucial role in hRPE cell migration and may be a potential therapeutic target against hRPE cell migration and PVR disease.
Source: Investigative Ophthalmology - October 29, 2013 Category: Opthalmology Authors: Qiu, S., Jiang, Z., Huang, Z., Chen, X., Qian, X., Gao, Q., Zheng, H. Tags: Retinal Cell Biology Source Type: research

Heat Shock Protein 27 Phosphorylation Is Involved in Epithelial Cell Apoptosis as well as Epithelial Migration during Corneal Epithelial Wound Healing.
In conclusion, the role of HSP27 in corneal epithelial wound healing can be epithelial cell apoptosis, as well as epithelial migration. HSP27 is involved in HCEC migration by the reorganization of actin cytoskeleton. PMID: 24239511 [PubMed - as supplied by publisher]
Source: Experimental Eye Research - November 14, 2013 Category: Opthalmology Authors: Song IS, Kang SS, Kim ES, Park HM, Choi CY, Tchah H, Kim JY Tags: Exp Eye Res Source Type: research

Role of Glutathione Peroxidase 4 in Conjunctival Epithelial Cells Cornea
Conclusion. These results suggest that GPx4 is essential for maintaining oxidative homeostasis and keeping defense against oxidative stress in conjunctival epithelial cells.
Source: Investigative Ophthalmology - January 21, 2015 Category: Opthalmology Authors: Sakai, O., Uchida, T., Imai, H., Ueta, T., Amano, S. Tags: Cornea Source Type: research