Filtered By:
Condition: Pregnancy

This page shows you your search results in order of relevance. This is page number 4.

Order by Relevance | Date

Total 418 results found since Jan 2013.

GnRH regulates trophoblast invasion via RUNX2-mediated MMP2/9 expression
STUDY HYPOTHESIS We hypothesized that Runt-related transcription factor 2 (RUNX2), matrix metalloproteinase (MMP)2 and MMP9 are involved in basal and gonadotrophin-releasing hormone (GnRH)-induced human extravillous trophoblast (EVT) cell invasion. STUDY FINDING Our finding indicates that GnRH-induced RUNX2 expression enhances the invasive capacity of EVT cells by modulating the expression of MMP2 and MMP9. WHAT IS KNOWN ALREADY GnRH is expressed in first-trimester placenta and exerts pro-invasive effects on EVT cells in vitro. RUNX2 regulates MMP2 and MMP9 expression and is often associated with invasive phenotypes. ST...
Source: Molecular Human Reproduction - January 30, 2016 Category: Molecular Biology Authors: Peng, B., Zhu, H., Klausen, C., Ma, L., Wang, Y.-l., Leung, P. C. K. Tags: Articles Source Type: research

Steroid receptor co-activator interacting protein (SIP) mediates EGF-stimulated expression of the prostaglandin synthase COX2 and prostaglandin release in human myometrium
STUDY HYPOTHESIS Steroid receptor coactivator interacting protein (SIP/KANK2) is involved in regulating the expression of the prostaglandin (PG)-endoperoxide synthase 2 (PTGS2; also known as cyclo-oxygenase 2, COX2) and PG release in human myometrium. STUDY FINDING SIP is phosphorylated in myometrial cells in response to epidermal growth factor (EGF)-stimulation and is required for EGF-stimulated increases in COX2 expression, PGE2 and PGF2α release, and expression of interleukins (IL) 6 and IL8. WHAT IS KNOWN ALREADY Human parturition involves inflammatory and non-inflammatory pathways and requires activation of th...
Source: Molecular Human Reproduction - July 11, 2016 Category: Molecular Biology Authors: Hudson, C. A., McArdle, C. A., Lopez Bernal, A. Tags: Original Research Source Type: research

nm23 regulates decidualization through the PI3K-Akt-mTOR signaling pathways in mice and humans
This study was supported by the National Natural Science Foundation of China (grant nos. 81370731, 31571551 and 31571190), the Science and Technology Project of Chongqing Education Committee (KJ130309), open funding by the Chongqing Institute for Family Planning (1201) and the Excellent Young Scholars of Chongqing Medical University (CQYQ201302). The authors have no conflicts of interest to declare.
Source: Human Reproduction - September 18, 2016 Category: Reproduction Medicine Authors: Zhang, X., Fu, L.-J., Liu, X.-Q., Hu, Z.-Y., Jiang, Y., Gao, R.-F., Feng, Q., Lan, X., Geng, Y.-Q., Chen, X.-M., He, J.-L., Wang, Y.-X., Ding, Y.-B. Tags: Reproductive biology Source Type: research

hCG activates Epac-Erk1/2 signaling regulating Progesterone Receptor expression and function in human endometrial stromal cells
AbstractSTUDY QUESTIONHow does hCG signal in human endometrial stromal cells (ESCs) and what is its role in regulating ESC function?SUMMARY ANSWERhCG signaling in ESCs activates the extracellular signal-regulated protein kinases 1 and 2 (Erk1/2) pathway through exchange protein activated by cyclic AMP (cAMP) (Epac) and transiently increases progesterone receptor (PR) transcript and protein expression and its transcriptional function.WHAT IS KNOWN ALREADYhCG is one of the earliest embryo-derived secreted signals in the endometrium, which abundantly expresses LH/hCG receptors. hCG signals through cAMP/protein kinase A (PKA) ...
Source: Molecular Human Reproduction - March 28, 2017 Category: Molecular Biology Source Type: research

WNK lysine deficient protein kinase 1 regulates human endometrial stromal cell decidualization, proliferation and migration in part through mitogen-activated protein kinase 7.
Abstract The differentiation of endometrial stromal cells into decidual cells, termed decidualization, is an integral step in the establishment of pregnancy. The mitogen-activated protein kinase homolog, WNK lysine deficient protein kinase 1 (WNK1), is activated downstream of epidermal growth factor receptor (EGFR) during decidualization. Primary human endometrial stromal cells (HESCs) were subjected to siRNA knockdown of WNK1 followed by in vitro decidualization. This abrogated expression of the decidual marker genes, IGFBP1 and PRL, and prevented adoption of decidual cell morphology. Analysis of the WNK1-depende...
Source: Biology of Reproduction - August 30, 2017 Category: Reproduction Medicine Authors: Adams NR, Vasquez YM, Mo Q, Gibbons W, Kovanci E, DeMayo FJ Tags: Biol Reprod Source Type: research

Up-regulation of miR-299 suppressed the invasion and migration of HTR-8/SVneo trophoblast cells partly via targeting HDAC2 in pre-eclampsia.
Abstract Pre-eclampsia (PE), a pregnancy-associated disorder, is a major contributor to maternal mortality and morbidity worldwide. Recently, microRNAs (miRNAs) were found to be associated with the pathogenesis of PE. The present study investigated the function of miR-299 in HTR-8/SVneo trophoblast cells and explored its underlying mechanism in the pathogenesis of PE. The miR-299 and histone deacetylase 2 (HDAC2) mRNA expression levels were determined by quantitative real-time PCR. Transwell invasion and wound healing assays were used to measure cell invasive and migratory ability. Luciferase reporter assay was pe...
Source: Biomedicine and pharmacotherapy = Biomedecine and pharmacotherapie - November 13, 2017 Category: Drugs & Pharmacology Authors: Gao Y, She R, Wang Q, Li Y, Zhang H Tags: Biomed Pharmacother Source Type: research

Reduced Expression of Hydrogen Sulfide-Generating Enzymes Down-Regulates 15-Hydroxyprostaglandin Dehydrogenase in Chorion  during Term and Preterm Labor.
Reduced Expression of Hydrogen Sulfide-Generating Enzymes Down-Regulates 15-Hydroxyprostaglandin Dehydrogenase in Chorion during Term and Preterm Labor. Am J Pathol. 2018 Jan;188(1):63-71 Authors: Sun Q, Chen Z, He P, Li Y, Ding X, Huang Y, Gu H, Ni X Abstract Chorionic NAD-dependent 15-hydroxyprostaglandin dehydrogenase (PGDH) plays a pivotal role in controlling the amount of prostaglandins in the uterus and has been implicated in the process of labor. Prior studies identified hydrogen sulfide-generating enzymes cystathionine-β-synthetase (CBS) and cystathionine-γ-lyase (CSE) in fetal membranes. ...
Source: The American Journal of Pathology - December 20, 2017 Category: Pathology Authors: Sun Q, Chen Z, He P, Li Y, Ding X, Huang Y, Gu H, Ni X Tags: Am J Pathol Source Type: research

CDKN1C (P57): one of the determinants of human endometrial stromal cell decidualization.
Abstract Decidualization is regulated by crosstalk of progesterone and the cAMP pathway. It involves extensive reprogramming of gene expression and includes a wide range of functions. To investigate how cell cycle regulatory genes drive the human endometrial stromal cell (ESC) exit cell cycle and enter differentiation, primary cultured ESC was treated with 8-Br-cAMP and MPA and cell cycle distribution was investigated by flow cytometry. High-throughput cell cycle regulatory gene expression was also studied by microarray. To validate the results of microarray chip, immunohistochemistry and semi-quantitative method ...
Source: Biology of Reproduction - January 9, 2018 Category: Reproduction Medicine Authors: Wang L, Yang H, Hu L, Hu D, Ma S, Sun X, Jiang L, Song J, Ji L, Masau JF, Zhang H, Qian K Tags: Biol Reprod Source Type: research

Association between DSCR4 gene methylation in plasma in early pregnancy and Down's syndrome.
Authors: Hu L, Zhou C Abstract Down's syndrome (DS), a chromosomal abnormal genetic disease caused by a local or total copy of chromosome 21, leads to patients suffering from delayed body growth, special facies, mild to moderate mental retardation and other symptoms, seriously affecting the life of patients. The aim of the present study was to examine the association between Down's syndrome critical region 4 (DSCR4) gene methylation in plasma in high-risk pregnant women with DS in early pregnancy (hereinafter referred to as pregnant women in early pregnancy) and DS, in order to screen new epigenetic markers for the...
Source: Experimental and Therapeutic Medicine - February 21, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Interaction of Porcine Reproductive and Respiratory Syndrome Virus major envelope proteins GP5 and M with the cellular protein Snapin.
CONCLUSIONS: The PRRSV GP5 and M proteins are known to form a heterodimeric complex which is important for viral structure and infectivity, and both PRRSV proteins can interact with cellular Snapin. Snapin knock-down suggests these interactions could be important in the PRRSV lifecycle. GP5 and M proteins may interact with Snapin to exploit its roles in intracellular transport and membrane fusion. PMID: 29577951 [PubMed - as supplied by publisher]
Source: Virus Research - March 22, 2018 Category: Virology Authors: Hicks JA, Yoo D, Liu HC Tags: Virus Res Source Type: research

GNA11 differentially mediates FGF2- and VEGFA-induced cellular responses in human fetoplacental endothelial cells.
This article is protected by copyright. All rights reserved. PMID: 29659033 [PubMed - as supplied by publisher]
Source: The Journal of Physiology - April 16, 2018 Category: Physiology Authors: Zou QY, Zhao YJ, Li H, Wang XZ, Liu AX, Zhong XQ, Yan Q, Li Y, Zhou C, Zheng J Tags: J Physiol Source Type: research

Nestin is highly expressed in fetal spinal cord isolated from placenta previa patients and promotes inflammation by enhancing NF- κB activity.
Conclusions Nestin is highly expressed and the interaction between nestin and CDK5 might lead to the progress of placenta previa through its regulation on NF-κB. PMID: 29697001 [PubMed - as supplied by publisher]
Source: Biomarkers - April 28, 2018 Category: Research Tags: Biomarkers Source Type: research

Molecules, Vol. 23, Pages 1085: Liposomes as Gene Delivery Vectors for Human Placental Cells
In this study, we evaluated the potential of liposomes as carriers for small interfering RNAs to placental cells. Three neutral formulations carrying rhodamine-labelled siRNAs were evaluated on an in vitro model, i.e., human primary villous cytotrophoblasts. siRNA internalization rate from lipoplexes were compared to the one in the presence of the lipofectamine reagent and assessed by confocal microscopy. Results showed cellular internalization of nucleic acid with all three formulations, based on two cationic lipids, either DMAPAP or CSL-3. Moreover, incubation with DMAPAP+AA provided a rate of labelled cells as high as w...
Source: Molecules - May 4, 2018 Category: Chemistry Authors: Lucie Valero Khair Alhareth Jenifer Espinoza Romero Warren Viricel Jeanne Leblond Audrey Chissey H élène Dhotel Caroline Roques Danielle Campiol Arruda Virginie Escriou Nathalie Mignet Thierry Fournier Karine Andrieux Tags: Communication Source Type: research

Role of PERK-eIF2 α signaling pathway in fetal male rats with hypospadias induced by di-n-butyl phthalate
This study aims to explore the role of PERK-eIF2α signaling pathway in fetal male rats with hypospadias induced by maternal exposure to di-n-butyl phthalate (DBP). DBP was used to treat pregnant SD rats by gastric intubation from gestation day (GD) 14–18 to construct a hypospadias rat model. The amount, weight, anogenital distance (AGD), and hypospadias incidence of rats were recorded and the genital tubercle (GT) of fetal male rats was collected on GD 19. Western blotting was performed to detect the expressions of PERK-eIF2α pathway- and autophagy-related proteins, and cell apoptosis was detected using TUNEL method. T...
Source: The Kaohsiung Journal of Medical Sciences - June 5, 2018 Category: Universities & Medical Training Source Type: research

Role of PERK-eIF2α signaling pathway in fetal male rats with hypospadias induced by di-n-butyl phthalate
This study aims to explore the role of PERK-eIF2α signaling pathway in fetal male rats with hypospadias induced by maternal exposure to di-n-butyl phthalate (DBP). DBP was used to treat pregnant SD rats by gastric intubation from gestation day (GD) 14–18 to construct a hypospadias rat model. The amount, weight, anogenital distance (AGD), and hypospadias incidence of rats were recorded and the genital tubercle (GT) of fetal male rats was collected on GD 19. Western blotting was performed to detect the expressions of PERK-eIF2α pathway- and autophagy-related proteins, and cell apoptosis was detected using TUNEL method. T...
Source: The Kaohsiung Journal of Medical Sciences - July 5, 2018 Category: Universities & Medical Training Source Type: research