Filtered By:
Condition: Arthritis

This page shows you your search results in order of relevance. This is page number 19.

Order by Relevance | Date

Total 425 results found since Jan 2013.

Histone deacetylase 3 regulates the inflammatory gene expression programme of rheumatoid arthritis fibroblast-like synoviocytes
Conclusions Inhibition of HDAC3 in RA FLS largely recapitulates the effects of pan-HDACi in suppressing inflammatory gene expression, including type I IFN production in RA FLS. Our results identify HDAC3 as a potential therapeutic target in the treatment of RA and type I IFN-driven autoimmune diseases.
Source: Annals of the Rheumatic Diseases - December 8, 2016 Category: Rheumatology Authors: Angiolilli, C., Kabala, P. A., Grabiec, A. M., Van Baarsen, I. M., Ferguson, B. S., Garcia, S., Malvar Fernandez, B., McKinsey, T. A., Tak, P. P., Fossati, G., Mascagni, P., Baeten, D. L., Reedquist, K. A. Tags: Open access Basic and translational research Source Type: research

SNAPIN is critical for lysosomal acidification and autophagosome maturation in macrophages.
Abstract We previously observed that SNAPIN, which is an adaptor protein in the SNARE core complex, was highly expressed in rheumatoid arthritis synovial tissue macrophages, but its role in macrophages and autoimmunity is unknown. To identify SNAPIN's role in these cells, we employed siRNA to silence the expression of SNAPIN in primary human macrophages. Silencing SNAPIN resulted in swollen lysosomes with impaired CTSD (cathepsin D) activation, although total CTSD was not reduced. Neither endosome cargo delivery nor lysosomal fusion with endosomes or autophagosomes was inhibited following the forced silencing of S...
Source: Autophagy - December 7, 2016 Category: Cytology Authors: Shi B, Huang QQ, Birkett R, Doyle R, Dorfleutner A, Stehlik C, He C, Pope RM Tags: Autophagy Source Type: research

TNFR1 and TNFR2 differentially mediate TNF- α-induced inflammatory responses in rheumatoid arthritis fibroblast-like synoviocytes.
TNFR1 and TNFR2 differentially mediate TNF-α-induced inflammatory responses in rheumatoid arthritis fibroblast-like synoviocytes. Cell Biol Int. 2017 Feb 02;: Authors: Zhang H, Xiao W Abstract TNF-α has long been implicated in the progression of rheumatoid arthritis (RA). However, how the receptors of TNF-α, namely TNFR1 and TNFR2, mediate TNF-α-induced inflammatory responses in fibroblast-like synoviocytes (FLS) in RA has not been elucidated. In the present study, primary FLS cells were isolated from RA patients and treated with TNF-α in vitro. The exogenous TNF-α induced the expression and rel...
Source: Cell Biology International - February 1, 2017 Category: Cytology Authors: Zhang H, Xiao W Tags: Cell Biol Int Source Type: research

Wogonin, a plant derived small molecule, exerts potent anti-inflammatory and chondroprotective effects through the activation of ROS/ERK/Nrf2 signaling pathways in human Osteoarthritis chondrocytes.
Abstract Osteoarthritis (OA), characterized by progressive destruction of articular cartilage, is the most common form of human arthritis. Here, we evaluated the potential chondroprotective and anti-inflammatory effects of Wogonin, a naturally occurring flavonoid, in IL-1β-stimulated human OA chondrocytes and cartilage explants. Wogonin completely suppressed the expression and production of inflammatory mediators including IL-6, COX-2, PGE2, iNOS and NO in IL-1β-stimulated OA chondrocytes. Further, Wogonin exhibits potent chondroprotective potential by switching the signaling axis of matrix degradation from cata...
Source: Free Radical Biology and Medicine - February 21, 2017 Category: Biology Authors: Khan NM, Haseeb A, Ansari MY, Devarapalli P, Haynie S, Haqqi TM Tags: Free Radic Biol Med Source Type: research

Resveratrol inhibits BK-induced COX-2 transcription by suppressing acetylation of AP-1 and NF- κB in human rheumatoid arthritis synovial fibroblasts.
Resveratrol inhibits BK-induced COX-2 transcription by suppressing acetylation of AP-1 and NF-κB in human rheumatoid arthritis synovial fibroblasts. Biochem Pharmacol. 2017 Mar 10;: Authors: Yang CM, Chen YW, Chi PL, Lin CC, Hsiao LD Abstract Bradykinin (BK) induces inflammation in rheumatoid arthritis (RA). Resveratrol is a potent activator of Sirt1 which could modulate inflammation through deacetylating histones of transcription factors. Here, we investigated the mechanisms underlying BK-induced COX-2 expression which is modulated by resveratrol/Sirt1 in human rheumatoid arthritis synovial fibrobla...
Source: Biochemical Pharmacology - March 9, 2017 Category: Drugs & Pharmacology Authors: Yang CM, Chen YW, Chi PL, Lin CC, Hsiao LD Tags: Biochem Pharmacol Source Type: research

SNX10 plays a critical role in MMP9 secretion via JNK ‐p38‐ERK signaling pathway
This article is protected by copyright. All rights reserved
Source: Journal of Cellular Biochemistry - May 12, 2017 Category: Biochemistry Authors: Chun Zhou, Ying Wang, Jin Peng, Cuixian Li, Peiqing Liu, Xiaoyan Shen Tags: Article Source Type: research

Therapeutic effect of Cryptotanshinone on experimental rheumatoid arthritis through downregulating p300 mediated-STAT3 acetylation.
CONCLUSION AND IMPLICATIONS: Our study suggested that the anti-arthritis effects of CTS were attained through suppression of p300-mediated STAT3 acetylation. Our data suggest that CTS might be a potential immune modulator for RA treatment. PMID: 28522406 [PubMed - as supplied by publisher]
Source: Biochemical Pharmacology - May 15, 2017 Category: Drugs & Pharmacology Authors: Wang Y, Zhou C, Gao H, Li C, Li D, Liu P, Huang M, Shen X, Liu L Tags: Biochem Pharmacol Source Type: research

TXNDC5 synergizes with HSC70 to exacerbate the inflammatory phenotype of synovial fibroblasts in rheumatoid arthritis through NF- κB signaling.
In this study, we show that TXNDC5 expression in RASFs and their cytokine production are significantly upregulated in response to LPS, TNF-α and IL-6, but suppressed by transfection with TXNDC5-siRNA. TXNDC5 is further validated as the direct target of NF-κB signaling. Mechanistically, TXNDC5 directly interacts with heat shock cognate 70 protein (HSC70) to sequester it in the cytoplasm, and HSC70 silencing exerts the same effects as TXNDC5 on the biological activity of RASFs (for example, decreased cell viability, invasion and cytokine production). Furthermore, HSC70 activates NF-κB signaling by destabilizing IκBβ pro...
Source: Cellular and Molecular Immunology - June 12, 2017 Category: Molecular Biology Authors: Wang L, Dong H, Song G, Zhang R, Pan J, Han J Tags: Cell Mol Immunol Source Type: research

Pneumolysin-induced autophagy contributes to inhibition of osteoblast differentiation through downregulation of Sp1 in human osteosarcoma cells
Conclusion: The results suggest that PLY inhibits osteoblast differentiation by downregulation of Sp1 accompanied by induction of autophagy through ROS-mediated regulation of the AMPK/mTOR pathway. General significance: This study proposes a molecular mechanism for inhibition of osteoblast differentiation in response to PLY.
Source: Biochimica et Biophysica Acta (BBA) General Subjects - July 19, 2017 Category: Biochemistry Source Type: research

Down ‐regulation of miR‐10a‐5p in synoviocytes contributes to TBX5‐controlled joint inflammation
Abstract MicroRNAs are considered to play critical roles in the pathogenesis of human inflammatory arthritis, including rheumatoid arthritis (RA). The purpose of this study was to determine the relationship between miR‐10a‐5p and TBX5 in synoviocytes and evaluate their contribution to joint inflammation. The expression of miR‐10a‐5p and TBX5 in the synovium of RA and human synovial sarcoma cell line SW982 stimulated by IL‐1β was determined by RT‐qPCR and Western blotting. The direct interaction between miR‐10a‐5p and TBX5 3′UTR was determined by dual‐luciferase reporter assay in HeLa cells. Mimics and ...
Source: Journal of Cellular and Molecular Medicine - August 7, 2017 Category: Molecular Biology Authors: Nazim Hussain, Wenhua Zhu, Congshan Jiang, Jing Xu, Xiaoying Wu, Manman Geng, Safdar Hussain, Yongsong Cai, Ke Xu, Peng Xu, Yan Han, Jian Sun, Liesu Meng, Shemin Lu Tags: Original Article Source Type: research

Down-regulation of miR-10a-5p in synoviocytes contributes to TBX5-controlled joint inflammation.
Abstract MicroRNAs are considered to play critical roles in the pathogenesis of human inflammatory arthritis, including rheumatoid arthritis (RA). The purpose of this study was to determine the relationship between miR-10a-5p and TBX5 in synoviocytes and evaluate their contribution to joint inflammation. The expression of miR-10a-5p and TBX5 in the synovium of RA and human synovial sarcoma cell line SW982 stimulated by IL-1β was determined by RT-qPCR and Western blotting. The direct interaction between miR-10a-5p and TBX5 3'UTR was determined by dual-luciferase reporter assay in HeLa cells. Mimics and inhibitors ...
Source: J Cell Mol Med - August 7, 2017 Category: Molecular Biology Authors: Hussain N, Zhu W, Jiang C, Xu J, Wu X, Geng M, Hussain S, Cai Y, Xu K, Xu P, Han Y, Sun J, Meng L, Lu S Tags: J Cell Mol Med Source Type: research

Expression of TREM-2 and its inhibitory effects on TNF- α induced inflammation in fibroblast-like synoviocytes via inhibiting p38 pathway activation.
CONCLUSIONS: TREM-2 expressed in RA-FLSs and TNF-α mediated reduction of inflammatory reactions. These phenomena indicated that TREM-2 may be a potential target in the treatment of RA. PMID: 28869414 [PubMed - as supplied by publisher]
Source: Clinical and Experimental Rheumatology - September 6, 2017 Category: Rheumatology Tags: Clin Exp Rheumatol Source Type: research

Endoplasmic reticulum stress and MAPK signaling pathway activation underlie leflunomide-induced toxicity in HepG2 Cells.
In this study, we used multiple approaches including genomic analysis to investigate and characterize the possible molecular mechanisms of the cytotoxicity of leflunomide in hepatic cells. We found that leflunomide caused endoplasmic reticulum (ER) stress and activated an unfolded protein response, as evidenced by increased expression of related genes including CHOP and GADD34; and elevated protein levels of typical ER stress markers including CHOP, ATF-4, p-eIF2α, and spliced XBP1. The secretion of Gaussia luciferase was suppressed in cells treated with leflunomide in an ER stress reporter assay. Inhibition of ER stress ...
Source: Toxicology - October 4, 2017 Category: Toxicology Authors: Ren Z, Chen S, Qing T, Xuan J, Couch L, Yu D, Ning B, Shi L, Guo L Tags: Toxicology Source Type: research

Fibroblast-like synoviocyte migration is enhanced by IL-17-mediated overexpression of L-type amino acid transporter 1 (LAT1) via the mTOR/4E-BP1 pathway.
In conclusion, further investigation is warranted into LAT1 as a potential target for drug therapies aimed at attenuating migration of transformed-appearing fibroblasts and subsequently preventing further erosion of bone and cartilage. PMID: 29198077 [PubMed - as supplied by publisher]
Source: Amino Acids - December 2, 2017 Category: Biochemistry Authors: Yu Z, Lin W, Rui Z, Jihong P Tags: Amino Acids Source Type: research

Proliferation and apoptosis of rheumatoid arthritis fibroblast ‐like synoviocytes following signal transducer and activator of transcription 3 RNA interference delivery
Conclusion: Transfection with targeted STAT3 recombinant plasmids effectively inhibited the expression of STAT3 mRNA and its protein in RA‐FLSs. RNAi‐mediated silencing of STAT3 reduced the proliferation and promoted the apoptosis of FLSs. This article is protected by copyright. All rights reserved
Source: Journal of Cellular Biochemistry - December 13, 2017 Category: Biochemistry Authors: Xuehui Sun, Yun Han, Ying Liu, Yanchun Tang, Jibo Wang Tags: Article Source Type: research