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STAT-3 regulation of CXCR4 is necessary for the prenylflavonoid Icaritin to enhance mesenchymal stem cell proliferation, migration and osteogenic differentiation
Publication date: Available online 19 April 2018 Source:Biochimica et Biophysica Acta (BBA) - General Subjects Author(s): R.Z.L. Lim, L. Li, E.L. Yong, N. Chew Mesenchymal stem cell (MSC) dysfunction has been implicated in the pathogenesis of osteoporosis. MSCs derived from osteoporotic subjects demonstrate significant impairment in proliferation, adhesion and chemotaxis, and osteogenic differentiation, leading to reduced functional bone-forming osteoblasts and ultimately nett bone loss and osteoporosis. Epimedium herbs and its active compound Icaritin (ICT) have been used in Chinese ethnopharmacology for the treatment of...
Source: Biochimica et Biophysica Acta (BBA) General Subjects - April 19, 2018 Category: Biochemistry Source Type: research

Deciphering the role of dimer interface in intrinsic dynamics and allosteric pathways underlying the functional transformation of DNMT3A
Conclusions Our results suggest that structural topology coupled with global motions indicates the structural origin of the functional transformation of DNMT3A. The comprehensive analysis further highlights the pivotal role of the dimer interface of DNMT3A both in defining the quaternary structure dynamics and establishing interdomain communications. General significance Understanding the global motions of DNMT3As not only provides mechanical insights into the functions of such molecular machines, but also reveals the mediators that determine their allosteric regulations. Graphical abstract (Source: Biochimica et Biophysic...
Source: Biochimica et Biophysica Acta (BBA) General Subjects - April 17, 2018 Category: Biochemistry Source Type: research

Diverse toxic effectors are harbored by vgrG islands for interbacterial antagonism in type VI secretion system
In this report, we first identified VT1, a novel amidase effector in ETEC, significantly hydrolyzed D-lactyl-L-Ala crosslinks between N-acetylmuramoyl and L-Ala in peptidoglycan. Further study showed that the VT1/VTI1 effector/immunity pair is encoded within a typical vgrG island, and plays a critical role for the successful establishment of ETEC in host gut. Numerous putative effectors with diverse toxin domains were found by retrieving vgrG islands in pathogenic E. coli, and designated as VT modules. Therein, VT5, a lysozyme-like effector widely encoded in ETEC, was confirmed to effectively kill adjacent cells, suggestin...
Source: Biochimica et Biophysica Acta (BBA) General Subjects - April 17, 2018 Category: Biochemistry Source Type: research

Localized conformational changes trigger the pH-induced fibrillogenesis of an amyloidogenic λ light chain protein
Publication date: Available online 15 April 2018 Source:Biochimica et Biophysica Acta (BBA) - General Subjects Author(s): Isabel Velázquez López, Gilberto Valdés-García, Sergio Romero Romero, Roberto Maya Martínez, Ana I. Leal-Cervantes, Miguel Costas, Rosana Sánchez-López, Carlos Amero, Nina Pastor, D. Alejandro Fernández Velasco Solvent conditions modulate the expression of the amyloidogenic potential of proteins. In this work the effect of pH on the fibrillogenic behavior and the conformational properties of 6aJL2, a model protein of the highly amyloidogenic varia...
Source: Biochimica et Biophysica Acta (BBA) General Subjects - April 15, 2018 Category: Biochemistry Source Type: research

Heparan sulfate 3-O-sulfotransferase 2 (HS3ST2) displays an unexpected subcellular localization in the plasma membrane
Conclusions We demonstrated that HS3ST3B is a Golgi-resident isozyme, while HS3ST2 is addressed to the plasma membrane with syndecan-2. General significance The membrane localization of HS3ST2 suggests that this enzyme may participate in discrete processes that occur at the cell surface. (Source: Biochimica et Biophysica Acta (BBA) General Subjects)
Source: Biochimica et Biophysica Acta (BBA) General Subjects - April 14, 2018 Category: Biochemistry Source Type: research

Selenoprotein synthesis and regulation in Archaea
Publication date: Available online 13 April 2018 Source:Biochimica et Biophysica Acta (BBA) - General Subjects Author(s): Michael Rother, Vivien Quitzke Background The major biological form of selenium is that of the co-translationally inserted amino acid selenocysteine (Sec). In Archaea, the majority of proteins containing Sec, selenoproteins, are involved in methanogenesis. However, the function of this residue is often not known because selenium-independent homologs of the selenoproteins can be employed, sometimes even in one organism. Scope of review This review summarizes current knowledge about the selenoproteins of...
Source: Biochimica et Biophysica Acta (BBA) General Subjects - April 14, 2018 Category: Biochemistry Source Type: research

Selenium-regulated hierarchy of human selenoproteome in cancerous and immortalized cells lines
Conclusions We characterized that selenoprotein hierarchy is cell-line specific with conserved features. This analysis should be done prior to any experiments in a novel cell line. General significance We reported a strategy based on complementary methods to evaluate selenoproteome regulation in human cells in culture. (Source: Biochimica et Biophysica Acta (BBA) General Subjects)
Source: Biochimica et Biophysica Acta (BBA) General Subjects - April 14, 2018 Category: Biochemistry Source Type: research

Selenium supplementation prevents metabolic and transcriptomic responses to cadmium in mouse lung
Conclusion Co-administration of Se with Cd prevented Cd increase in lung and prevented Cd-associated pathway and network responses of the transcriptome and metabolome. Se protection against Cd toxicity in lung involves complex systems responses. General significance Environmental Cd stimulates proinflammatory and profibrotic signaling. The present results indicate that dietary or supplemental Se could be useful to mitigate Cd toxicity. (Source: Biochimica et Biophysica Acta (BBA) General Subjects)
Source: Biochimica et Biophysica Acta (BBA) General Subjects - April 13, 2018 Category: Biochemistry Source Type: research

Molecular mechanism of selenoprotein P synthesis
Publication date: Available online 12 April 2018 Source:Biochimica et Biophysica Acta (BBA) - General Subjects Author(s): Sumangala Shetty, Paul R. Copeland Background Selenoprotein synthesis requires the reinterpretation of a UGA stop codon as one that encodes selenocysteine (Sec), a process that requires a set of dedicated translation factors. Among the mammalian selenoproteins, Selenoprotein P (SELENOP) is unique as it contains a selenocysteine-rich domain that requires multiple Sec incorporation events. Scope of review In this review we elaborate on new data and current models that provide insight into how SELENOP is ...
Source: Biochimica et Biophysica Acta (BBA) General Subjects - April 13, 2018 Category: Biochemistry Source Type: research

Interference of carbidopa and other catechols with reactions catalyzed by peroxidases
Conclusions Tested compounds reduced the oxidation products or intermediates of model substrates thus preventing chromophore formation. This interference may affect interpretation of results of diagnostic tests in samples from patients with Parkinson's disease treated with carbidopa and l-dopa. General significance This mechanism allows prediction of interference in peroxidase-based diagnostic tests for other compounds, including drugs and natural products. Graphical abstract (Source: Biochimica et Biophysica Acta (BBA) General Subjects)
Source: Biochimica et Biophysica Acta (BBA) General Subjects - April 10, 2018 Category: Biochemistry Source Type: research

14-3-3 protein directly interacts with the kinase domain of calcium/calmodulin-dependent protein kinase kinase (CaMKK2)
Conclusions 14-3-3γ protein directly interacts with the kinase domain of CaMKK2 and the region containing the inhibitory phosphorylation site Thr145 within the N-terminal extension. General significance Our results suggested that CaMKK isoforms differ in their 14-3-3-mediated regulations and that the interaction between 14 and 3-3 protein and the N-terminal 14-3-3-binding motif of CaMKK2 might be stabilized by small-molecule compounds. Graphical abstract (Source: Biochimica et Biophysica Acta (BBA) General Subjects)
Source: Biochimica et Biophysica Acta (BBA) General Subjects - April 10, 2018 Category: Biochemistry Source Type: research

Integrating in vitro and in silico approaches to evaluate the “dual functionality” of palmatine chloride in inhibiting and disassembling Tau-derived VQIVYK peptide fibrils
Conclusions We found that PC possesses “dual functionality” towards PHF6 and full-length tau, i.e. inhibit their aggregation and disassemble pre-formed fibrils. General significance The “dual functionality” of PC is valuable as a disease modifying strategy for AD, and other tauopathies, by inhibiting their progress and reducing the effect of fibrils already present in the brain. Graphical abstract (Source: Biochimica et Biophysica Acta (BBA) General Subjects)
Source: Biochimica et Biophysica Acta (BBA) General Subjects - April 8, 2018 Category: Biochemistry Source Type: research

Synergistic cellular responses to heavy metal exposure: A minireview
Publication date: Available online 7 April 2018 Source:Biochimica et Biophysica Acta (BBA) - General Subjects Author(s): Chanyoung Park, Jeeyon Jeong Background Metal-responsive transcription factor 1 (MTF-1) induces the expression of metallothioneins (MTs) which bind and sequester labile metal ions. While MTF-1 primarily responds to excess metal exposure, additional stress response mechanisms are activated. Evidence suggests potential crosstalk between responses mediated by MTF-1 and stress signaling enhances cellular tolerance to metal exposure. Scope of review This review aims to summarize current understanding of the ...
Source: Biochimica et Biophysica Acta (BBA) General Subjects - April 8, 2018 Category: Biochemistry Source Type: research

High affinity sugar ligands of C-type lectin receptor langerin
Conclusions L4 is a specific ligand for langerin. Oligomerization of L4 unit increased the affinity toward langerin. General significance These results suggest that oligomeric L4 derivatives will be useful for clarifying the langerin functions and for the development of new glycan-based anti-inflammatory drugs. (Source: Biochimica et Biophysica Acta (BBA) General Subjects)
Source: Biochimica et Biophysica Acta (BBA) General Subjects - April 8, 2018 Category: Biochemistry Source Type: research

Quantitative biochemical characterization and biotechnological production of caspase modulator, XIAP: Therapeutic implications for apoptosis-associated diseases
Conclusions We have determined that HtrA2 specifically binds to the BIR2 and BIR3 of XIAP at a 1:1 molecular ratio. Moreover, in vitro cell-free CASP-9 and -3/7 activation-apoptosis assays have demonstrated that these purified XIAP proteins dramatically inhibit CASP-9 and -3/7 action. General significance Our system is suitable for biochemical studies, such as quantitation of the number of molecules acting on the apoptosis regulation, and provides a basis and insights that can be applied to the development of therapeutic agents for neurodegenerative disorders and cancer. (Source: Biochimica et Biophysica Acta (BBA) General Subjects)
Source: Biochimica et Biophysica Acta (BBA) General Subjects - April 8, 2018 Category: Biochemistry Source Type: research

Investigation into the intracellular fates, speciation and mode of action of selenium-containing neuroprotective agents using XAS and XFM
Conclusions The differences in activity were thus attributed to the varied speciation and cellular localisation of the compounds, or their metabolites, as detected by X-ray absorption spectroscopy and X-ray fluorescence microscopy. Significance The work is significant as it links, for the first time, the protective action of selenium compounds against redox stress with particular chemical speciation using a direct measurement approach. (Source: Biochimica et Biophysica Acta (BBA) General Subjects)
Source: Biochimica et Biophysica Acta (BBA) General Subjects - April 8, 2018 Category: Biochemistry Source Type: research

Potential role for Ext1-dependent heparan sulfate in regulating P311 gene expression in A549 carcinoma cells
This study considers a possible novel mechanism of Ext1-regulated heparan sulfate structure in modifying tumor-stroma interactions through altering stromal tgf-ß1 expression. (Source: Biochimica et Biophysica Acta (BBA) General Subjects)
Source: Biochimica et Biophysica Acta (BBA) General Subjects - April 7, 2018 Category: Biochemistry Source Type: research

Regulation and function of avian selenogenome
Publication date: Available online 5 April 2018 Source:Biochimica et Biophysica Acta (BBA) - General Subjects Author(s): Shuping Li, Fei Gao, Jiaqiang Huang, Yuanyuan Wu, Sen Wu, Xin Gen Lei Background Selenium (Se) is an essential micronutrient required by avian species. Dietary Se/vitamin E deficiency induces three classical diseases in chicks: exudative diathesis, nutritional pancreatic atrophy, and nutritional muscular dystrophy. Scope of review This review is to summarize and analyze the evolution, regulation, and function of avian selenogenome and selenoproteome and their relationship with the three classical Se/vit...
Source: Biochimica et Biophysica Acta (BBA) General Subjects - April 6, 2018 Category: Biochemistry Source Type: research

Combined thermodynamic and kinetic analysis of GroEL interacting with CXCR4 transmembrane peptides
Publication date: Available online 5 April 2018 Source:Biochimica et Biophysica Acta (BBA) - General Subjects Author(s): Haixia Chi, Baomei Xu, Zhenzhen Liu, Junting Wei, Shixin Li, Hao Ren, Yan Xu, Xinwei Lu, Xiaojuan Wang, Xiaoqiang Wang, Fang Huang GroEL along with ATP and its co-chaperonin GroES has been demonstrated to significantly enhance the folding of newly translated G-protein-coupled receptors (GPCRs). This work extends the previous studies to explore the guest capture and release processes in GroEL-assisted folding of GPCRs, by the reduced approach of employing CXCR4 transmembrane peptides as model substrates....
Source: Biochimica et Biophysica Acta (BBA) General Subjects - April 6, 2018 Category: Biochemistry Source Type: research

Characteristic glycopeptides associated with extreme human longevity identified through plasma glycoproteomics
Conclusions Tri-antennary and sialylated N-glycans on haptoglobin at the Asn207 and Asn211 sites were abundant in SSCs and characteristic of extreme human longevity. General significance We found abundant glycans in SSCs, which may be associated with human longevity. (Source: Biochimica et Biophysica Acta (BBA) General Subjects)
Source: Biochimica et Biophysica Acta (BBA) General Subjects - April 6, 2018 Category: Biochemistry Source Type: research

Mechanisms of selenium hyperaccumulation in plants: A survey of molecular, biochemical and ecological cues
Publication date: Available online 4 April 2018 Source:Biochimica et Biophysica Acta (BBA) - General Subjects Author(s): Leonardo Warzea Lima, Elizabeth A.H. Pilon-Smits, Michela Schiavon Background Selenium (Se) is a micronutrient required for many life forms, but toxic at higher concentration. Plants do not have a Se requirement, but can benefit from Se via enhanced antioxidant activity. Some plant species can accumulate Se to concentrations above 0.1% of dry weight and seem to possess mechanisms that distinguish Se from its analog sulfur (S). Research on these so-called Se hyperaccumulators aims to identify key genes f...
Source: Biochimica et Biophysica Acta (BBA) General Subjects - April 5, 2018 Category: Biochemistry Source Type: research

Selenium-dependent gene expression in Methanococcus maripaludis: Involvement of the transcriptional regulator HrsM
Conclusions HrsM exerts a key role in regulating expression of selenoprotein genes and their Cys-encoding isogenes in M. maripaludis in a selenium-dependent fashion. However, this activity is not achieved via autoregulation but probably by a mechanism, which modulates the DNA-binding of HrsM. General significance Although LTTRs are abundant in Bacteria, HrsM represents only the second characterized member of this group in Archaea. (Source: Biochimica et Biophysica Acta (BBA) General Subjects)
Source: Biochimica et Biophysica Acta (BBA) General Subjects - April 5, 2018 Category: Biochemistry Source Type: research

Spontaneous intracranial hypotension is diagnosed by a combination of lipocalin-type prostaglandin D synthase and brain-type transferrin in cerebrospinal fluid
Conclusion L-PGDS and brain-type Tf can be biomarkers for diagnosing SIH. General significance L-PGDS and brain-type Tf biosynthesized in the brain appears to be markers for abnormal metabolism of CSF. (Source: Biochimica et Biophysica Acta (BBA) General Subjects)
Source: Biochimica et Biophysica Acta (BBA) General Subjects - April 3, 2018 Category: Biochemistry Source Type: research

Conformational properties of intrinsically disordered proteins bound to the surface of silica nanoparticles
Conclusions IDPs maintain structural disorder inside HC, experiencing minor, protein-specific, induced folding and stabilization against further conformational transitions, such as formation of intermolecular beta-sheets upon dehydration. The HC is formed by a single layer of protein molecules. SC likely plays a key role stabilizing amyloidogenic α-synuclein conformers. General significance Protein-NP interactions can mimic those with macromolecular partners, allowing dissection of contributing factors by rational design of NP surfaces. Application of NPs in vivo should be carefully tested for amyloidogenic potential...
Source: Biochimica et Biophysica Acta (BBA) General Subjects - April 3, 2018 Category: Biochemistry Source Type: research

Cdk5 suppression blocks SIRT1 degradation via the ubiquitin-proteasome pathway in Parkinson's disease models
In conclusion, our findings revealed that the Cdk5-dependent ubiquitin-proteasome pathway mediated degradation of SIRT1 plays a vital role in the progression of PD. (Source: Biochimica et Biophysica Acta (BBA) General Subjects)
Source: Biochimica et Biophysica Acta (BBA) General Subjects - March 31, 2018 Category: Biochemistry Source Type: research

Oleuropein aglycone: A polyphenol with different targets against amyloid toxicity
Conclusions This study reveals that OleA remodels not only D76N b2m aggregates but also the cell membrane interfering with the misfolded proteins-cell membrane association, in most cases an early event triggering amyloid–mediated cytotoxicity. General significance The data provided in the present article focus on OleA protection, featuring this polyphenol as a promising plant molecule useful against amyloid diseases. Graphical abstract (Source: Biochimica et Biophysica Acta (BBA) General Subjects)
Source: Biochimica et Biophysica Acta (BBA) General Subjects - March 31, 2018 Category: Biochemistry Source Type: research

The NC domain of HIV-1 Gag contributes to the interaction of Gag with TSG101
Conclusion The NC zinc fingers and p6 domain of Gag participate in the formation of the Gag-TSG101 complex and in its cellular localisation. General significance This study illustrates that the NC and p6 domains cooperate in the interaction with TSG101 during HIV-1 budding. In addition, details on the Gag-TSG101 complex were obtained by combining two high resolution biophysical techniques. (Source: Biochimica et Biophysica Acta (BBA) General Subjects)
Source: Biochimica et Biophysica Acta (BBA) General Subjects - March 28, 2018 Category: Biochemistry Source Type: research

Structural basis of the signal transduction via transmembrane domain of the human growth hormone receptor
Conclusions Two alternative dimeric structures of GHR TMD attributed to dormant and active receptor states interchange via allosteric rearrangements of transmembrane helices and extracellular juxtamembrane regions that support coordination between protein-protein and protein-lipid interactions. General significance This study provides a holistic vision of GHR signal transduction across the membrane emphasizing the role of protein-lipid interactions. Graphical abstract (Source: Biochimica et Biophysica Acta (BBA) General Subjects)
Source: Biochimica et Biophysica Acta (BBA) General Subjects - March 28, 2018 Category: Biochemistry Source Type: research

Differential effects on enzyme stability and kinetic parameters of mutants related to human triosephosphate isomerase deficiency
Publication date: June 2018 Source:Biochimica et Biophysica Acta (BBA) - General Subjects, Volume 1862, Issue 6 Author(s): Nallely Cabrera, Alfredo Torres-Larios, Itzhel García-Torres, Sergio Enríquez-Flores, Ruy Perez-Montfort Human triosephosphate isomerase (TIM) deficiency is a very rare disease, but there are several mutations reported to be causing the illness. In this work, we produced nine recombinant human triosephosphate isomerases which have the mutations reported to produce TIM deficiency. These enzymes were characterized biophysically and biochemically to determine their kinetic and stability par...
Source: Biochimica et Biophysica Acta (BBA) General Subjects - March 27, 2018 Category: Biochemistry Source Type: research

Effects of volatile anaesthetics on heme metabolism in a murine genetic model of Acute Intermittent Porphyria. A comparative study with other porphyrinogenic drugs
Discussion Biochemical alterations caused by the porphyrinogenic drugs assayed were different in male and female mice and also between T1 and AIP being more affected the females of AIP group. General significance This is the first study using volatile anaesthetics in an AIP genetic model confirming Isoflurane and Sevoflurane porphyrinogenicity. (Source: Biochimica et Biophysica Acta (BBA) General Subjects)
Source: Biochimica et Biophysica Acta (BBA) General Subjects - March 21, 2018 Category: Biochemistry Source Type: research

Functional characterization and FTIR-based 3D modeling of full length and truncated forms of Scorpio maurus venom phospholipase A2
Conclusion Infrared spectroscopy data and 3D modeling confirm the experimental findings that highlight the importance of the short chain for the Sm-PLGV activity. General significance New informations are given to further establish the structure-function relationships of the Sm-PLGV. (Source: Biochimica et Biophysica Acta (BBA) General Subjects)
Source: Biochimica et Biophysica Acta (BBA) General Subjects - March 20, 2018 Category: Biochemistry Source Type: research

Investigation of structure and function of mitochondrial alcohol dehydrogenase isozyme III from Komagataella phaffii GS115
Conclusions KpADH3 plays an important role in the metabolism of alcohols in Komagataella phaffii GS115, and its crystal structure is the only dimeric medium-chain ADH from yeast described so far. General significance Knowledge of the relationship between structure and function of KpADH3 is crucial for understanding the role of KpADH3 in Komagataella phaffii GS115 mitochondrial metabolism. Graphical abstract (Source: Biochimica et Biophysica Acta (BBA) General Subjects)
Source: Biochimica et Biophysica Acta (BBA) General Subjects - March 20, 2018 Category: Biochemistry Source Type: research

Releasing iron from ferritin protein nanocage by reductive method: The role of electron transfer mediator
Conclusions Reductive mobilization of iron from ferritin is dependent on the relative rate of NADH oxidation, dissolved O2 consumption and mineral core reduction, which in turn depends on E1/2 of these mediators and their interaction with ferritin. General significance The current mechanism of in vitro iron mobilization from ferritin by using redox mediators involves different ET steps, which may help to understand the iron release pathway in vivo and to check microbial growth. Graphical abstract (Source: Biochimica et Biophysica Acta (BBA) General Subjects)
Source: Biochimica et Biophysica Acta (BBA) General Subjects - March 20, 2018 Category: Biochemistry Source Type: research

Tyr120Asp mutation alters domain flexibility and dynamics of MeCP2 DNA binding domain leading to impaired DNA interaction: Atomistic characterization of a Rett syndrome causing mutation
Publication date: May 2018 Source:Biochimica et Biophysica Acta (BBA) - General Subjects, Volume 1862, Issue 5 Author(s): Ilda D'Annessa, Anna Gandaglia, Elena Brivio, Gilda Stefanelli, Angelisa Frasca, Nicoletta Landsberger, Daniele Di Marino Mutations in the X-linked MECP2 gene represent the main origin of Rett syndrome, causing a profound intellectual disability in females. MeCP2 is an epigenetic transcriptional regulator containing two main functional domains: a methyl-CpG binding domain (MBD) and a transcription repression domain (TRD). Over 600 pathogenic mutations were reported to affect the whole protein; almost h...
Source: Biochimica et Biophysica Acta (BBA) General Subjects - March 20, 2018 Category: Biochemistry Source Type: research

Optical fiber tips for biological applications: From light confinement, biosensing to bioparticles manipulation
Publication date: May 2018 Source:Biochimica et Biophysica Acta (BBA) - General Subjects, Volume 1862, Issue 5 Author(s): Joana S. Paiva, Pedro A.S. Jorge, Carla C. Rosa, João P.S. Cunha Background The tip of an optical fiber has been considered an attractive platform in Biology. The simple cleaved end of an optical fiber can be machined, patterned and/or functionalized, acquiring unique properties enabling the exploitation of novel optical phenomena. Prompted by the constant need to measure and manipulate nanoparticles, the invention of the Scanning Near-field Optical Microscopy (SNOM) triggered the optimization a...
Source: Biochimica et Biophysica Acta (BBA) General Subjects - March 20, 2018 Category: Biochemistry Source Type: research

On elongation factor eEFSec, its role and mechanism during selenium incorporation into nascent selenoproteins
This article is part of a Special Issue entitled Selenium research in biochemistry and biophysics - 200 year anniversary issue, edited by Dr. Elias Arnér and Dr. Regina Brigelius-Flohe. (Source: Biochimica et Biophysica Acta (BBA) General Subjects)
Source: Biochimica et Biophysica Acta (BBA) General Subjects - March 18, 2018 Category: Biochemistry Source Type: research

Transforming growth factor- β modulates pancreatic cancer associated fibroblasts cell shape, stiffness and invasion
Conclusions TGF-β modulates CAFs', but not FBs', cell shape, stiffness and invasion. General Significance Our findings elucidate on the effects of TGF-β on CAFs' behavior and stiffness providing new insights into the mechanisms involved. Graphical abstract (Source: Biochimica et Biophysica Acta (BBA) General Subjects)
Source: Biochimica et Biophysica Acta (BBA) General Subjects - March 16, 2018 Category: Biochemistry Source Type: research

Changes in the stability and biomechanics of P22 bacteriophage capsid during maturation
Publication date: Available online 15 March 2018 Source:Biochimica et Biophysica Acta (BBA) - General Subjects Author(s): Ravi Kant, Aida Llauró, Vamseedhar Rayaprolu, Shefah Qazi, Pedro J. de Pablo, Trevor Douglas, Brian Bothner The capsid of P22 bacteriophage undergoes a series of structural transitions during maturation that guide it from spherical to icosahedral morphology. The transitions include the release of scaffold proteins and capsid expansion. Although P22 maturation has been investigated for decades, a unified model that incorporates thermodynamic and biophysical analyses is not available. A general an...
Source: Biochimica et Biophysica Acta (BBA) General Subjects - March 16, 2018 Category: Biochemistry Source Type: research

Stress-induced acidification may contribute to formation of unusual structures in C9orf72-repeats
Conclusions In the proper sequence context, i-motif structures can form at pH values found in some cells in vivo. General Significance DNA conformational plasticity exists over broad range of solution conditions. (Source: Biochimica et Biophysica Acta (BBA) General Subjects)
Source: Biochimica et Biophysica Acta (BBA) General Subjects - March 16, 2018 Category: Biochemistry Source Type: research

A ketogenic amino acid rich diet benefits mitochondrial homeostasis by altering the AKT/4EBP1 and autophagy signaling pathways in the gastrocnemius and soleus
Publication date: Available online 15 March 2018 Source:Biochimica et Biophysica Acta (BBA) - General Subjects Author(s): Jinpeng Li, Megumi Kanasaki, Ling Xu, Munehiro Kitada, Kenji Nagao, Yusuke Adachi, Hiroko Jinzu, Yasushi Noguchi, Miyuki Kohno, Keizo Kanasaki, Daisuke Koya Muscle biology is important topic in diabetes research. We have reported that a diet with ketogenic amino acids rich replacement (KAAR) ameliorated high-fat diet (HFD)-induced hepatosteatosis via activation of the autophagy system. Here, we found that a KAAR ameliorated the mitochondrial morphological alterations and associated mitochondrial dysfun...
Source: Biochimica et Biophysica Acta (BBA) General Subjects - March 16, 2018 Category: Biochemistry Source Type: research

Structural and biochemical insights into the substrate-binding mechanism of a novel glycoside hydrolase family 134 β-mannanase
Publication date: Available online 14 March 2018 Source:Biochimica et Biophysica Acta (BBA) - General Subjects Author(s): Xin You, Zhen Qin, Yan-Xiao Li, Qiao-Juan Yan, Bin Li, Zheng-Qiang Jiang Mannan is one of the major constituent groups of hemicellulose, which is a renewable resource from higher plants. β-Mannanases are enzymes capable of degrading lignocellulosic biomass. Here, an endo-β-mannanase from Rhizopus microsporus (RmMan134A) was cloned and expressed. The recombinant RmMan134A showed maximal activity at pH 5.0 and 50 °C, and exhibited high specific activity towards locust bean gum (2337 U...
Source: Biochimica et Biophysica Acta (BBA) General Subjects - March 15, 2018 Category: Biochemistry Source Type: research

Cell wall integrity is required for pullulan biosynthesis and glycogen accumulation in Aureobasidium melanogenum P16
In this study, the GT6 and GT7 genes encoding different α1,2 mannosyltransferases in Aureobasidium melanogenum P16 were cloned and characterized. The proteins deduced from both the GT6 and GT7 genes contained the conserved sequences YNMCHFWSNFEI and YSTCHFWSNFEI of a Ktr mannosyltransferase family. The removal of each gene and both the two genes caused the changes in colony and cell morphology and enhanced glycogen accumulation, leading to a reduced pullulan biosynthesis and the declined expression of many genes related to pullulan biosynthesis. The swollen cells of the disruptants were due to increased accumulation ...
Source: Biochimica et Biophysica Acta (BBA) General Subjects - March 15, 2018 Category: Biochemistry Source Type: research

Comparison of ATP sulfurylase 2 from selenium hyperaccumulator Stanleya pinnata and non-accumulator Stanleya elata reveals differential intracellular localization and enzyme activity levels
Conclusions The ATPS2 from S. pinnata differs from non-accumulator ATPS2 in its elevated expression and sub-cellular localization. It likely acts on both selente and sulfate substrates. General significance These observations shed new light on the role of ATPS2 in the evolution of Se hyperaccumulation in plants. This article is part of a Special Issue entitled Selenium research in biochemistry and biophysics - 200 year anniversary issue, edited by Dr. Elias Arnér and Dr. Regina Brigelius-Flohe. (Source: Biochimica et Biophysica Acta (BBA) General Subjects)
Source: Biochimica et Biophysica Acta (BBA) General Subjects - March 15, 2018 Category: Biochemistry Source Type: research

A subset of functional adaptation mutations alter propensity for α-helical conformation in the intrinsically disordered glucocorticoid receptor tau1core activation domain
Conclusions Changes in PreSMo stability account for the effects of a subset of change-of-function mutants affecting the GR tau1core IDR. General significance Long IDRs occur in about 50% of human proteins. They are poorly characterized despite much recent attention. Our results suggest the importance of a subtle balance between PreSMo stability and IDR activity, which may provide a novel target for future pharmaceutical intervention. (Source: Biochimica et Biophysica Acta (BBA) General Subjects)
Source: Biochimica et Biophysica Acta (BBA) General Subjects - March 15, 2018 Category: Biochemistry Source Type: research

Membrane-lipid homeostasis in a prodromal rat model of Alzheimer's disease: Characteristic profiles in ganglioside distributions during aging detected using MALDI imaging mass spectrometry
Conclusions These results are indicative of a pathological shift in ganglioside homeostasis during aging that is exacerbated in Tg APP21 rats. General significance Ganglioside dysregulation may occur in the prodromal stages of neurodegenerative diseases like AD. (Source: Biochimica et Biophysica Acta (BBA) General Subjects)
Source: Biochimica et Biophysica Acta (BBA) General Subjects - March 14, 2018 Category: Biochemistry Source Type: research

Endoglin based in vivo near-infrared fluorescence imaging of tumor models in mice using activatable liposomes
Conclusions The work highlights key differences between targeting vascular (murine) and neoplastic (human) endoglin in animal studies, and suggests that the hEnd-IL can serve as a delivery system that targets human endoglin overexpressed in pathological conditions. General Significance. The endoglin-targeting liposomes presented herewith represent strategic tools for the future implementation of endoglin-directed neoplastic and anti-angiogenic therapies. Graphical abstract (Source: Biochimica et Biophysica Acta (BBA) General Subjects)
Source: Biochimica et Biophysica Acta (BBA) General Subjects - March 14, 2018 Category: Biochemistry Source Type: research

Polyethylene glycol promotes autoxidation of cytochrome c
Publication date: Available online 11 March 2018 Source:Biochimica et Biophysica Acta (BBA) - General Subjects Author(s): Wataru Sato, Takeshi Uchida, Tomohide Saio, Koichiro Ishimori Cytochrome c (Cyt c) was rapidly oxidized by molecular oxygen in the presence, but not absence of PEG. The redox potential of heme c was determined by the potentiometric titration to be +236 ± 3 mV in the absence of PEG, which was negatively shifted to +200 ± 4 mV in the presence of PEG. The underlying the rapid oxidation was explored by examining the structural changes in Cyt c in the presence of PEG using UV&ndash...
Source: Biochimica et Biophysica Acta (BBA) General Subjects - March 12, 2018 Category: Biochemistry Source Type: research

Amphipathic helical peptides hamper protein-protein interactions of the intrinsically disordered chromatin nuclear protein 1 (NUPR1)
Conclusions Designed peptides can be used as lead compounds to inhibit NUPR1 interactions. General significance Peptides may be exploited as drugs to target IDPs. Graphical abstract (Source: Biochimica et Biophysica Acta (BBA) General Subjects)
Source: Biochimica et Biophysica Acta (BBA) General Subjects - March 11, 2018 Category: Biochemistry Source Type: research

Stereochemical and structural effects of (2R,6R)-hydroxynorketamine on the mitochondrial metabolome in PC-12 cells
Conclusions The present data provide an overall view of the metabolic changes in mitochondrial function produced by (2R,6R)-HNK and related ketamine compounds and offer an insight into the source of the observed variance in antidepressant response elicited by the compounds. Graphical abstract (Source: Biochimica et Biophysica Acta (BBA) General Subjects)
Source: Biochimica et Biophysica Acta (BBA) General Subjects - March 10, 2018 Category: Biochemistry Source Type: research

Imaging linear and circular polarization features in leaves with complete Mueller matrix polarimetry
Publication date: Available online 9 March 2018 Source:Biochimica et Biophysica Acta (BBA) - General Subjects Author(s): C.H. Lucas Patty, David A. Luo, Frans Snik, Freek Ariese, Wybren Jan Buma, Inge Loes ten Kate, Rob J.M. van Spanning, William B. Sparks, Thomas A. Germer, Győző Garab, Michael W. Kudenov Spectropolarimetry of intact plant leaves allows to probe the molecular architecture of vegetation photosynthesis in a non-invasive and non-destructive way and, as such, can offer a wealth of physiological information. In addition to the molecular signals due to the photosynthetic machinery, the cell structure and its...
Source: Biochimica et Biophysica Acta (BBA) General Subjects - March 10, 2018 Category: Biochemistry Source Type: research