A bacterial 2[4Fe4S] ferredoxin as redox partner of the plastidic-type ferredoxin-NADP+ reductase from Leptospira interrogans
ConclusionsWe report for the first time a redox pair in L. interrogans in which a plastidic FNR exchanges electron with a bacterial 2[4Fe4S] ferredoxin. We characterized this reaction and proposed a model for the productive LepFNR-LepFd2 complex.General significanceOur findings suggest that the interaction of LepFNR with the iron-sulfur protein would be different from the one previously described for the homolog enzymes. This knowledge would be useful for the design of specific LepFNR inhibitors.Graphical abstract (Source: Biochimica et Biophysica Acta (BBA) General Subjects)
Source: Biochimica et Biophysica Acta (BBA) General Subjects - January 11, 2019 Category: Biochemistry Source Type: research

Covalent inhibition of histone deacetylase 8 by 3,4-dihydro-2H-pyrimido[1,2-c][1,3]benzothiazin-6-imine
Conclusions1 and its analogs are interesting model compounds but unsuitable for therapeutic treatment due to their high unselective reactivity towards thiol groups. However, the postulated preceding non-covalent binding mode of 1 opens a door to optimized next generation compounds that combine potent and selective non-covalent recognition with low reactivity towards C153 at the active site of HDAC8.General significance1 represents a completely new class of inhibitors for HDAC8. Initial non-covalent interaction at the bottom of the active site is suggested to be the key for its selectivity. Further optimization of non-coval...
Source: Biochimica et Biophysica Acta (BBA) General Subjects - January 4, 2019 Category: Biochemistry Source Type: research

Identification and characterization of phytocannabinoids as novel dual PPARα/γ agonists by a computational and in vitro experimental approach
ConclusionsOur work broadens the activity spectrum of CBDA, CBGA and CBG by providing evidence that these pCBs act as dual PPARα/γ agonists with the ability to modulate the lipid metabolism.General Significance.Dual PPARα/γ agonists have emerged as an attractive alternative to selective PPAR agonists to treat metabolic disorders. We identified some pCBs as dual PPARα/γ agonists, potentially useful for the treatment of dyslipidemia and type 2 diabetes mellitus.Graphical Abstract (Source: Biochimica et Biophysica Acta (BBA) General Subjects)
Source: Biochimica et Biophysica Acta (BBA) General Subjects - January 4, 2019 Category: Biochemistry Source Type: research

Structure-based design and application of an engineered glutathione transferase for the development of an optical biosensor for pesticides determination
Publication date: Available online 24 December 2018Source: Biochimica et Biophysica Acta (BBA) - General SubjectsAuthor(s): Evangelia G. Chronopoulou, Dimitrios Vlachakis, Anastassios C. Papageorgiou, Farid S. Ataya, Nikolaos E. LabrouAbstractIn the present work, a structure-based design approach was used for the generation of a novel variant of synthetic glutathione transferase (PvGmGSTU) with higher sensitivity towards pesticides. Molecular modelling studies revealed Phe117 as a key residue that contributes to the formation of the hydrophobic binding site (H-site) and modulates the affinity of the enzyme towards xenobiot...
Source: Biochimica et Biophysica Acta (BBA) General Subjects - December 25, 2018 Category: Biochemistry Source Type: research

Editorial Board
Publication date: February 2019Source: Biochimica et Biophysica Acta (BBA) - General Subjects, Volume 1863, Issue 2Author(s): (Source: Biochimica et Biophysica Acta (BBA) General Subjects)
Source: Biochimica et Biophysica Acta (BBA) General Subjects - December 25, 2018 Category: Biochemistry Source Type: research

Phosphoinositide-3-kinase inhibition elevates ferritin level resulting depletion of labile iron pool and blocking of glioma cell proliferation
ConclusionsPI3K inhibition promotes ferritin synthesis by dual mechanism resulting sequestration of iron to limit its availability for cell proliferation in glioma cells but not in primary astrocytes.General Significance: This observation establishes a relation between PI3K signalling and iron homeostasis in glioma cells. It also implies that activated PI3K controls ferritin expression to ensure availability of adequate iron required for cell proliferation.Graphical abstract (Source: Biochimica et Biophysica Acta (BBA) General Subjects)
Source: Biochimica et Biophysica Acta (BBA) General Subjects - December 24, 2018 Category: Biochemistry Source Type: research

Molecular dynamics simulations of the chemokine CCL2 in complex with pull down-derived heparan sulfate hexasaccharides
ConclusionsA discrimination of specific vs. unspecific CCL2 GAG ligands is not possible by this approach. Long-time molecular dynamics simulations are, however, well suited to capture the delicate enthalpy/entropy balance of GAG binding and improve results obtained from docking.General significance.With the comparison of two methods, MS-based ligand identification and molecular modelling, we have shown the current limitations of our molecular understanding of complex ligand binding which is could be due to the numerical inaccessibility of ligand-induced protein conformational changes. (Source: Biochimica et Biophysica Acta...
Source: Biochimica et Biophysica Acta (BBA) General Subjects - December 24, 2018 Category: Biochemistry Source Type: research

The Regulation of TORC1 pathway by the yeast chaperones Hsp31 is mediated by SFP1 and affects proteasomal activity
Publication date: Available online 19 December 2018Source: Biochimica et Biophysica Acta (BBA) - General SubjectsAuthor(s): C.A. Padilla, J.A. Bárcena, M.J. López-Grueso, R. Requejo-AguilarAbstractThe Saccharomyces cerevisiae heat shock proteins Hsp31–34 are members of DJ-1/ThiJ/Pfpl superfamily that includes human DJ-1 (Park7), a protein involved in heritable Parkinsonism. Although, homologs of these proteins can be found in most organisms their functions are unclear. We have carried out a quantitative proteomics analysis of yeast cells devoid of the whole set of Hsp31 family of proteins, as a model of...
Source: Biochimica et Biophysica Acta (BBA) General Subjects - December 19, 2018 Category: Biochemistry Source Type: research

A novel method for cloning of coding sequences of highly toxic proteins
ConclusionsThe designed method allows cloning of toxic protein coding sequences that cannot be cloned by standard methods.General Significance.Cloning of cDNAs encoding toxic proteins is still a troublesome problem that hinders the progress of numerous studies. The method described here is a convenient solution to cloning problems that are common in research on toxic proteins.Graphical Abstract (Source: Biochimica et Biophysica Acta (BBA) General Subjects)
Source: Biochimica et Biophysica Acta (BBA) General Subjects - December 19, 2018 Category: Biochemistry Source Type: research

M(en)TORship lessons on life and death by the integrated stress response
Publication date: Available online 17 December 2018Source: Biochimica et Biophysica Acta (BBA) - General SubjectsAuthor(s): Antonis E. KoromilasAbstractCells employ pro-survival and pro-adaptive pathways to cope with different forms of environmental stress. When stress is excessive, and the damage caused by it is unsustainable, cells engage pro-death pathways, which are in place to protect the host from the deleterious effects of harmed cells. Two important pathways that determine the balance between survival and death of stressed cells are the integrated stress response (ISR) and the mammalian target of rapamycin (mTOR), ...
Source: Biochimica et Biophysica Acta (BBA) General Subjects - December 18, 2018 Category: Biochemistry Source Type: research

Deficiency of α1,6-fucosyltransferase promotes neuroinflammation by increasing the sensitivity of glial cells to inflammatory mediators
Conclusions: Core fucosylation negatively regulates the states of neuroinflammation by modulating the sensitivity of microglia and astrocytes to inflammatory mediators. The disorders of Fut8−/− mice are caused not only by neurons but also by glial cell dysfunction.General significance: Core fucose is a novel regulator for neuroinflammation in the central nervous system. (Source: Biochimica et Biophysica Acta (BBA) General Subjects)
Source: Biochimica et Biophysica Acta (BBA) General Subjects - December 18, 2018 Category: Biochemistry Source Type: research

Missing-in-metastasis protein promotes internalization of magnetic nanoparticles via association with clathrin light chain and Rab7
ConclusionsMIM regulates internalization of MNPs via promoting their trafficking from plasma membrane to late endosomes.General significanceOur data unveiled a novel pathway which MNPs internalization and intracellular trafficking in macrophages. This new pathway may allow us to control the uptake of MNPs within cells by targeting MIM, thereby improving their medical applications.Graphical abstract (Source: Biochimica et Biophysica Acta (BBA) General Subjects)
Source: Biochimica et Biophysica Acta (BBA) General Subjects - December 14, 2018 Category: Biochemistry Source Type: research

Human αS1-casein induces IL-8 secretion by binding to the ecto-domain of the TLR4/MD2 receptor complex
ConclusionHuman αS1-casein induced proinflammatory effects are dependent upon binding to the TLR4 ectodomain and the presence of CD14. αS1-casein displayed stronger TLR4 agonistic activity than LPS via a different mode of action.General significanceBreast milk protein αS1-casein is a proinflammatory cytokine.Graphical abstract (Source: Biochimica et Biophysica Acta (BBA) General Subjects)
Source: Biochimica et Biophysica Acta (BBA) General Subjects - December 14, 2018 Category: Biochemistry Source Type: research

Structural alteration of glycosaminoglycan side chains and spatial disorganization of collagen networks in the skin of patients with mcEDS-CHST14
Publication date: Available online 13 December 2018Source: Biochimica et Biophysica Acta (BBA) - General SubjectsAuthor(s): Takuya Hirose, Naoki Takahashi, Prasarn Tangkawattana, Jun Minaguchi, Shuji Mizumoto, Shuhei Yamada, Noriko Miyake, Shujiro Hayashi, Atsushi Hatamochi, Jun Nakayama, Tomomi Yamaguchi, Ayana Hashimoto, Yoshihiro Nomura, Kazushige Takehana, Tomoki Kosho, Takafumi WatanabeAbstractMusculocontractural Ehlers-Danlos syndrome (mcEDS) due to CHST14/D4ST1 deficiency (mcEDS-CHST14) is a recently delineated type of EDS caused by biallelic loss-of-function mutations in CHST14, which results in the depletion of de...
Source: Biochimica et Biophysica Acta (BBA) General Subjects - December 14, 2018 Category: Biochemistry Source Type: research

Uridine diphosphate release mechanism in O-N-acetylglucosamine (O-GlcNAc) transferase catalysis
Publication date: Available online 11 December 2018Source: Biochimica et Biophysica Acta (BBA) - General SubjectsAuthor(s): Nai She, Yuan Zhao, Jingjing Hao, Songqiang Xie, Chaojie WangAbstractO-linked N-acetylglucosamine transferase (OGT) is an essential enzyme that catalyzes the covalent bonding of N-acetylglucosamine (GlcNAc) to the hydroxyl group of a serine or threonine in the target protein. It plays an important role in many important cellular physiological catalytic reactions. Here, we determine the binding mode and the binding free energy of the OGT product (uridine diphosphate, UDP) as well as the hydrogen-bond-d...
Source: Biochimica et Biophysica Acta (BBA) General Subjects - December 12, 2018 Category: Biochemistry Source Type: research

Dynamic interactions between RNA viruses and human hosts unravelled by a decade of next generation sequencing
Publication date: Available online 6 December 2018Source: Biochimica et Biophysica Acta (BBA) - General SubjectsAuthor(s): Chaturaka Rodrigo, Fabio LucianiAbstractBackgroundNext generation sequencing (NGS) methods have significantly contributed to a paradigm shift in genomic research for nearly a decade now. These methods have been useful in studying the dynamic interactions between RNA viruses and human hosts.Scope of the review.In this review, we summarise and discuss key applications of NGS in studying the host – pathogen interactions in RNA viral infections of humans with examples.Major conclusions.Use of NGS to ...
Source: Biochimica et Biophysica Acta (BBA) General Subjects - December 7, 2018 Category: Biochemistry Source Type: research

TRIM8-driven transcriptomic profile of neural stem cells identified glioma-related nodal genes and pathways
ConclusionsOur results substantiate the role of TRIM8 in the brain functions through the dysregulation of genes involved in different CNS-related pathways, including JAK-STAT.General significanceThis study provides novel insights on the physiological TRIM8 function by profiling for the first time the primary Neural Stem Cell over-expressing TRIM8 by using RNA-Sequencing methodology. (Source: Biochimica et Biophysica Acta (BBA) General Subjects)
Source: Biochimica et Biophysica Acta (BBA) General Subjects - December 6, 2018 Category: Biochemistry Source Type: research

38Missing-in-metastasis protein promotes internalization of magnetic nanoparticles via association with clathrin light chain and Rab7
ConclusionsMIM regulates internalization of MNPs via promoting their trafficking from plasma membrane to late endosomes.General significance.Our data unveiled a novel pathway which MNPs internalization and intracellular trafficking in macrophages. This new pathway may allow us to control the uptake of MNPs within cells by targeting MIM, thereby improving their medical applications.Graphical abstract (Source: Biochimica et Biophysica Acta (BBA) General Subjects)
Source: Biochimica et Biophysica Acta (BBA) General Subjects - December 6, 2018 Category: Biochemistry Source Type: research

Therapeutic effects of rosuvastatin in hypercholesterolemic prediabetic mice in the absence of low density lipoprotein receptor
Publication date: Available online 30 November 2018Source: Biochimica et Biophysica Acta (BBA) - General SubjectsAuthor(s): Erika Gurzeler, Einari Aavik, Anssi Laine, Teemu Valkama, Henri Niskanen, Jenni Huusko, Minna U. Kaikkonen, Seppo Ylä-HerttualaAbstractStatins are effective drugs used to prevent and treat cardiovascular diseases but their effects in the absence of low density lipoprotein receptor (LDLR) and on the risk of diabetes are not yet well characterized. The aim of this study was to clarify systemic and pleiotropic effects of rosuvastatin on cardiovascular and diabetic phenotypes.IGF-II/LDLR−/&minu...
Source: Biochimica et Biophysica Acta (BBA) General Subjects - December 1, 2018 Category: Biochemistry Source Type: research

The thioredoxin-mediated recycling of Arabidopsis thaliana GRXS16 relies on a conserved C-terminal cysteine
ConclusionThe reduction of AtGRXS16, which is mandatory for its oxidoreductase activity and the binding of FeS clusters, depends on light through the plastidial FTR/TRX system. Hence, disulfide formation may constitute a redox switch mechanism controlling GRXS16 function in response to day/night transition or oxidizing conditions.General significance.From the in vitro data obtained with roGFP2, one can postulate that GRXS16 would mediate protein glutathionylation/oxidation in plastids but not their deglutathionylation. (Source: Biochimica et Biophysica Acta (BBA) General Subjects)
Source: Biochimica et Biophysica Acta (BBA) General Subjects - November 29, 2018 Category: Biochemistry Source Type: research

G-quadruplex formation by single-base mutation or deletion of mitochondrial DNA sequences
ConclusionsSingle-base mutations of mt10251 could make significant changes in its structural variation and some single-base mutated sequences in mtDNA could form G4 structures in vitro.General significanceWe illustrate the importance of single-base mutations of DNA sequences to the change of G4 formation in vitro. The use of single-base mutations by generating the fourth G-tract and followed by selection in shortening the longest loop size in the near-but-not-PQF sequences was conducted for the G4 formation.Graphical abstract (Source: Biochimica et Biophysica Acta (BBA) General Subjects)
Source: Biochimica et Biophysica Acta (BBA) General Subjects - November 27, 2018 Category: Biochemistry Source Type: research

Mechanistic insights into metal ions transit through threefold ferritin channel
ConclusionsOverall, the study provides insights into Fe2+ entry mechanism and characteristic features of metal-protein interactions that influence the metal ions passage. The dynamics data suggest that E130 may act as a metal selectivity gate. This implicates an ion-specific entry mechanism through the channel with the distinct diffusion kinetics being the discriminating factor.General Significance: Ferritin nanocages not only act as biological iron reservoirs but also have gained importance in material science as template scaffolds for synthesizing metal nanoparticles. This study provides mechanistic understanding on the ...
Source: Biochimica et Biophysica Acta (BBA) General Subjects - November 27, 2018 Category: Biochemistry Source Type: research

Selective ϖ-1 oxidation of fatty acids by CYP147G1 from Mycobacterium marinum
ConclusionsThis selectivity of fatty acid hydroxylation inferred that linear species must bind in the active site of the enzyme with the terminal methyl group sequestered so that abstraction at the CH bonds of the ω-1 position is favoured. With branched substrates, one of the methyl groups must be close to the compound I oxygen atom and enable hydroxylation at the terminal methyl group to compete with the reaction at the ω-1C-H bond.General Significance.Hydroxy fatty acids are widely used for industrial, food and medical purposes. CYP147G1 demonstrates high regioselectivity for hydroxylation at a sub-terminal p...
Source: Biochimica et Biophysica Acta (BBA) General Subjects - November 24, 2018 Category: Biochemistry Source Type: research

The Iron Islands: Erythroblastic Islands and Iron Metabolism
Conclusion.Despite being studied for over 60 years, the mechanisms by which the erythroblastic island niche serves to control erythroid cell iron metabolism are poorly resolved.General Significance.Over 2 billion people suffer from some form of anaemia. Iron deficiency anaemia is the most prevalent form of anaemia. Therefore, understanding the processes by which iron is trafficked to, and metabolised in developing erythrocytes, is crucially important. (Source: Biochimica et Biophysica Acta (BBA) General Subjects)
Source: Biochimica et Biophysica Acta (BBA) General Subjects - November 22, 2018 Category: Biochemistry Source Type: research

Sp140 is a multi-SUMO-1 target and its PHD finger promotes SUMOylation of the adjacent Bromodomain
ConclusionsSp140 is multi-SUMOylated and its PHD finger works as versatile protein-protein interaction platform promoting intramolecular SUMOylation of the adjacent BRD. Thus, combinatorial association of Sp140 chromatin binding domains generates a multifaceted interaction scaffold, whose function goes beyond the canonical histone recognition.General significanceThe addition of Sp140 to the increasing lists of multi-SUMOylated proteins opens new perspectives for molecular studies on Sp140 transcriptional activity, where SUMOylation could represent a regulatory route and a docking surface for the recruitment and assembly of...
Source: Biochimica et Biophysica Acta (BBA) General Subjects - November 21, 2018 Category: Biochemistry Source Type: research

IL-15 improves skeletal muscle oxidative metabolism and glucose uptake in association with increased respiratory chain supercomplex formation and AMPK pathway activation
ConclusionsAcute IL-15 treatment that mimics local IL-15 concentrations enhances muscle glucose uptake and mitochondrial oxidative functions. That mitochondria respond differently to different levels of IL-15 during chronic treatments indicates that IL-15 might activate two different pathways in muscle depending on IL-15 concentrations.General significance.Our results suggest that IL-15 may act in an autocrine/paracrine fashion and be, at least in part, involved in the positive effect of exercise on muscle energy metabolism. (Source: Biochimica et Biophysica Acta (BBA) General Subjects)
Source: Biochimica et Biophysica Acta (BBA) General Subjects - November 16, 2018 Category: Biochemistry Source Type: research

Intermolecular crosslinking of abnormal prion protein is efficiently induced by a primuline-sensitized photoreaction
Publication date: Available online 14 November 2018Source: Biochimica et Biophysica Acta (BBA) - General SubjectsAuthor(s): Kenta Teruya, Keiko Nishizawa, Ayumi Oguma, Yuji Sakasegawa, Tetsuyuki Kitamoto, Katsumi Doh-uraAbstractIn prion diseases, infectious pathogenic particles that are composed of abnormal prion proteins (PrPSc) accumulate in the brain. PrPSc is biochemically characterized by its protease-resistance core (PrPres), but its structural features have not been fully elucidated. Here, we report that primuline, a fluorescent dye with photosensitization activity, dramatically enhances UV-irradiation-induced SDS-r...
Source: Biochimica et Biophysica Acta (BBA) General Subjects - November 15, 2018 Category: Biochemistry Source Type: research

The thioredoxin system as a target for mercury compounds
Conclusions.The mechanism of toxicity for mercurials is a complex chain of events starting with inhibition of the selenoenzyme, thioredoxin reductase (TrxR). Selenium supplementation protects TrxR from the toxicity of inorganic forms of mercury (i.e.Hg(II)) to a certain extent, but not from methylmercury.When TrxR is inhibited, thioredoxin is reduced by alternative mechanisms involving glutathione and glutaredoxin and only when this pathway is hampered does cell death occur.General Significance.The understanding of the molecular mechanism of mercury toxicity and mechanisms of enzymatic compensation allows the design of mit...
Source: Biochimica et Biophysica Acta (BBA) General Subjects - November 15, 2018 Category: Biochemistry Source Type: research

Earthworm protease in anti-thrombosis and anti-fibrosis
Publication date: Available online 13 November 2018Source: Biochimica et Biophysica Acta (BBA) - General SubjectsAuthor(s): Xiu-Mei Wang, Shi-Chao Fan, Yao Chen, Xiao-Feng Ma, Rong-Qiao HeAbstractBackgroundEarthworms are widely used in basic and applied research in medicine, food, environment and agriculture, in which for instance earthworm protease has its own biochemical features.Scope of reviewThis review summarizes earthworm protease biochemical features in anti-thrombosis and anti-fibrosis, and provides new perspectives for earthworm to be used in biochemical and pharmaceutical studies.Major conclusionsEarthworm prote...
Source: Biochimica et Biophysica Acta (BBA) General Subjects - November 14, 2018 Category: Biochemistry Source Type: research

Role of leucine zipper-like motifs in the oligomerization of Pseudomonas putida phasins
ConclusionsThese results indicate that coiled-coil motifs are essential for phasin interactions. Correct oligomerization requires the formation of a stable hydrophobic interface between both phasins.General Significance.Our findings elucidate the oligomerization motif of PhaF and PhaI. This motif is present in most phasins from PHA-accumulating bacteria and offers a potentially important target for modulating the PHA granules stability.Graphical abstract (Source: Biochimica et Biophysica Acta (BBA) General Subjects)
Source: Biochimica et Biophysica Acta (BBA) General Subjects - November 11, 2018 Category: Biochemistry Source Type: research

Interaction of the anticancer p28 peptide with p53-DBD as studied by fluorescence, FRET, docking and MD simulations
Conclusionsp28 binds to DBD partially engaging the L1 loop, at the same region of the p53 down-regulator COP1, leaving however the DNA binding site available for functional interactions.General significanceElucidation of the DBD-p28 complex gets insights into the functional role of p28 in regulating the p53 anticancer activity, also offering new perspectives to design new drugs able to protect the p53 anticancer function.Graphical abstract (Source: Biochimica et Biophysica Acta (BBA) General Subjects)
Source: Biochimica et Biophysica Acta (BBA) General Subjects - November 11, 2018 Category: Biochemistry Source Type: research

Successful amphiphiles as the key to crystallization of membrane proteins: Bridging theory and practice
Publication date: Available online 10 November 2018Source: Biochimica et Biophysica Acta (BBA) - General SubjectsAuthor(s): Joscha Breibeck, Annette RompelAbstractBackgroundMembrane proteins constitute a major group of proteins and are of great significance as pharmaceutical targets, but underrepresented in the Protein Data Bank. Particular reasons are their low expression yields and the constant need for cautious and diligent handling in a sufficiently stable hydrophobic environment substituting for the native membrane. When it comes to protein crystallization, such an environment is often established by detergents.Scope ...
Source: Biochimica et Biophysica Acta (BBA) General Subjects - November 11, 2018 Category: Biochemistry Source Type: research

Mechanisms underlying acquired platinum resistance in high grade serous ovarian cancer - a mini review
Publication date: Available online 10 November 2018Source: Biochimica et Biophysica Acta (BBA) - General SubjectsAuthor(s): Mudra Binju, Monica Amaya Padilla, Terence Singomat, Pritinder Kaur, Yohan Suryo Rahmanto, Paul Cohen, Yu YuAbstractBackgroundAdvanced epithelial ovarian cancer is one of the hardest human malignancies to treat. Standard treatment involves cytoreductive surgery and platinum-based chemotherapy, however, median progression-free survival for patients diagnosed with advanced stage disease (FIGO stages III and IV) is approximately 18 months. There has been little improvement in overall survival over the ...
Source: Biochimica et Biophysica Acta (BBA) General Subjects - November 11, 2018 Category: Biochemistry Source Type: research

Editorial Board
Publication date: January 2019Source: Biochimica et Biophysica Acta (BBA) - General Subjects, Volume 1863, Issue 1Author(s): (Source: Biochimica et Biophysica Acta (BBA) General Subjects)
Source: Biochimica et Biophysica Acta (BBA) General Subjects - November 11, 2018 Category: Biochemistry Source Type: research

Reviewer Acknowledgement
Publication date: January 2019Source: Biochimica et Biophysica Acta (BBA) - General Subjects, Volume 1863, Issue 1Author(s): (Source: Biochimica et Biophysica Acta (BBA) General Subjects)
Source: Biochimica et Biophysica Acta (BBA) General Subjects - November 11, 2018 Category: Biochemistry Source Type: research

Destabilised human transthyretin shapes the morphology of calcium carbonate crystals
Publication date: February 2019Source: Biochimica et Biophysica Acta (BBA) - General Subjects, Volume 1863, Issue 2Author(s): Elżbieta Wieczorek, Anna Chitruń, Andrzej OżyharAbstractHuman transthyretin (TTR) is a homotetramer that transports thyroid hormones and retinol in the serum and cerebrospinal fluid. TTR is also an intracellular protein found in tissues such as those in the brain, eye and pancreas. TTR is a nutrition marker, reflecting the health of the organism, and TTR levels are linked to the normal and diseased states of the body. The switch from a protective to a pathological role is attributed to the destab...
Source: Biochimica et Biophysica Acta (BBA) General Subjects - November 11, 2018 Category: Biochemistry Source Type: research

Structural Studies and Biological Evaluation of T30695 Variants Modified with Single Chiral Glycerol-T Reveal the Importance of LEDGF/p75 for the Aptamer Anti-HIV-Integrase Activities
Publication date: Available online 7 November 2018Source: Biochimica et Biophysica Acta (BBA) - General SubjectsAuthor(s): Elisa Rivieccio, Luciana Tartaglione, Veronica Esposito, Carmela Dell'Aversano, P.C. Koneru, Maria Scuotto, Antonella Virgilio, Luciano Mayol, Mamuka Kvaratskhelia, Michela VarraAbstractSome G-quadruplex (GQ) forming aptamers, such as T30695, exhibit particularly promising properties among the potential anti-HIV drugs. T30695 G-quadruplex binds to HIV-1 integrase (IN) and inhibits its activity during 3′-end processing at nanomolar concentrations. Herein we report a study concerning six T30695-G...
Source: Biochimica et Biophysica Acta (BBA) General Subjects - November 8, 2018 Category: Biochemistry Source Type: research

Mesoporous silica templated-albumin nanoparticles with high doxorubicin payload for drug delivery assessed with a 3-D tumor cell model
Publication date: Available online 2 November 2018Source: Biochimica et Biophysica Acta (BBA) - General SubjectsAuthor(s): Mathilde Ménard, Florent Meyer, Ksenia Parkhomenko, Cédric Leuvrey, Grégory Francius, Sylvie Bégin-Colin, Damien MertzAbstractHuman serum albumin (HSA) nanoparticles emerge as promising carriers for drug delivery. Among challenges, one important issue is the design of HSA nanoparticles with a low mean size of ca. 50 nm and having a high drug payload. The original strategy developed here is to use sacrificial mesoporous nanosilica templates having a diameter close to 30 n...
Source: Biochimica et Biophysica Acta (BBA) General Subjects - November 3, 2018 Category: Biochemistry Source Type: research

Chemotaxis by Pseudomonas putida (ATCC 17453) towards camphor involves cytochrome P450cam (CYP101A1)
Publication date: Available online 1 November 2018Source: Biochimica et Biophysica Acta (BBA) - General SubjectsAuthor(s): Priyadarshini Balaraman, Erika PlettnerAbstractThe camphor-degrading microorganism, Pseudomonas putida strain ATCC 17453, is an aerobic, gram-negative soil bacterium that uses camphor as its sole carbon and energy source. The genes responsible for the catabolic degradation of camphor are encoded on the extra-chromosomal CAM plasmid. A monooxygenase, cytochrome P450cam, mediates hydroxylation of camphor to 5-exo-hydroxycamphor as the first and committed step in the camphor degradation pathway, requiring...
Source: Biochimica et Biophysica Acta (BBA) General Subjects - November 2, 2018 Category: Biochemistry Source Type: research

Multiple low-level exposures: Hg interactions with co-occurring neurotoxic substances in early life
Publication date: Available online 29 October 2018Source: Biochimica et Biophysica Acta (BBA) - General SubjectsAuthor(s): José G. DóreaAbstractAll chemical forms of Hg can affect neurodevelopment; however, low levels of organic Hg (methylmercury-MeHg and ethylmercury-EtHg in Thimerosal-containing vaccines, hereafter ‘TCV’) exposures during early life (pregnancy and lactation) co-occur with other environmental neurotoxic substances. These neurotoxicants may act in parallel, synergistically, or antagonistically to Hg. Nevertheless, the risks of neurotoxicity associated with multiple neuro-toxicants...
Source: Biochimica et Biophysica Acta (BBA) General Subjects - October 30, 2018 Category: Biochemistry Source Type: research

Lifetime-based photoconversion of EGFP as a tool for FLIM
ConclusionsBesides potential FLIM artifacts caused by an unintended EGFP photoconversion, the controlled photoconversion turns EGFP to an excellent tool for kinetic FLIM applications. Since the photoconversion occurs in the lifetime domain, PC-EGFP can be easily distinguished from the unconverted tag by time-resolved detection while all other spectral channels stay free for multicolor labeling.GENERAL SIGNIFICANCE.The reported lifetime photoconversion lines up EGFP with other photoconvertible fluorescent proteins with special advantage for fluorescence lifetime imaging where lifetime-photoconvertible labels are scarce.Grap...
Source: Biochimica et Biophysica Acta (BBA) General Subjects - October 29, 2018 Category: Biochemistry Source Type: research

Destabilised human transthyretin shapes the morphology of calcium carbonate crystals.
Publication date: Available online 28 October 2018Source: Biochimica et Biophysica Acta (BBA) - General SubjectsAuthor(s): Elżbieta Wieczorek, Anna Chitruń, Andrzej OżyharAbstractHuman transthyretin (TTR) is a homotetramer that transports thyroid hormones and retinol in the serum and cerebrospinal fluid. TTR is also an intracellular protein found in tissues such as those in the brain, eye and pancreas. TTR is a nutrition marker, reflecting the health of the organism, and TTR levels are linked to the normal and diseased states of the body. The switch from a protective to a pathological role is attributed to the destabili...
Source: Biochimica et Biophysica Acta (BBA) General Subjects - October 29, 2018 Category: Biochemistry Source Type: research

Biomedical potential of chitosan-silver nanoparticles with special reference to antioxidant, antibacterial, hemolytic and in vivo cutaneous wound healing effects
Publication date: January 2019Source: Biochimica et Biophysica Acta (BBA) - General Subjects, Volume 1863, Issue 1Author(s): Sawssen Hajji, Sameh Ben Khedir, Ibtissem Hamza-Mnif, Marwa Hamdi, Ines Jedidi, Rim Kallel, Sami Boufi, Moncef NasriAbstractThe aim of the present study was to prepare chitosan-PVA-silver nanoparticles (CS-AgNPs) through green method. Chitosan and PVA polymers acted as stabilizing agents. DLS and TEM analyses showed that CS-AgNPs were homogeneously dispersed in matrix with an average size of 190–200 nm. The CS-AgNPs were tested for their antioxidant and antibacterial properties and the result...
Source: Biochimica et Biophysica Acta (BBA) General Subjects - October 29, 2018 Category: Biochemistry Source Type: research

Methacycline displays a strong efficacy in reducing toxicity in a SCA3 Caenorhabditis elegans model
ConclusionsThe apparent absence of toxic effects displayed by methacycline, along with its stronger efficacy in contrasting expanded ataxin-3 toxicity, makes it a possible candidate for a chronic treatment of the disease. (Source: Biochimica et Biophysica Acta (BBA) General Subjects)
Source: Biochimica et Biophysica Acta (BBA) General Subjects - October 27, 2018 Category: Biochemistry Source Type: research

Characterization of a nucleus located mollusc mitoferrin and its response to OsHV-1 infection
In this study, a unique mollusc mitoferrin gene was identified in ark clams, named SbmiFn, that showed conserved three-dimensional structure with other mitoferrins, and its iron binding activity was verified by iron chelating assay. Besides cytoplasmic distribution, colocalization between SbmiFn and nuclei was observed by immunohistochemistry assay. Moreover, the response of SbmiFn to viral pathogen OsHV-1 was investigated. The results showed that nucleus located signal of SbmiFn was enhanced, the expressions of SbmiFn and ferritin were coordinately decreased, which might assist host against OsHV-1 replication as the incre...
Source: Biochimica et Biophysica Acta (BBA) General Subjects - October 20, 2018 Category: Biochemistry Source Type: research

Dimerization of an aptamer generated from Ligand-guided selection (LIGS) yields a high affinity scaffold against B-cells
Publication date: Available online 17 October 2018Source: Biochimica et Biophysica Acta (BBA) - General SubjectsAuthor(s): Sana Batool, Kimon V. Argyropoulos, Roksana Azad, Precious Okeoma, Hasan Zumrut, Sanam Bhandari, Rigzin Dekhang, Prabodhika R. MallikaratchyAbstractNucleic Acid Aptamers (NAAs) are a class of synthetic DNA or RNA molecules that bind specifically to their target. We recently introduced an aptamer termed R1.2 against membrane Immunoglobulin M (mIgM) expressing B-cell neoplasms using Ligand Guided Selection (LIGS). While LIGS-generated aptamers are highly specific, their lower affinity prevents aptamers f...
Source: Biochimica et Biophysica Acta (BBA) General Subjects - October 18, 2018 Category: Biochemistry Source Type: research

Microwave characterization of low-molecular-weight antioxidant specific biomarkers
Publication date: Available online 17 October 2018Source: Biochimica et Biophysica Acta (BBA) - General SubjectsAuthor(s): Natalia Naumova, Hanna Hlukhova, Alexander Barannik, Alexey Gubin, Irina Protsenko, Nickolay Cherpak, Svetlana VitusevichAbstractAntioxidants play a crucial role in the life sciences, as the regulators of biochemical reactions. We studied the dielectric properties of the low-molecular weight antioxidant specific biomarkers sodium ascorbate and glutathione in solutions of different concentrations. The biomarkers are multifunctional metabolites relevant to the reactive oxygen species (ROS) scavenging sys...
Source: Biochimica et Biophysica Acta (BBA) General Subjects - October 18, 2018 Category: Biochemistry Source Type: research

Biomedical potential of chitosan‑silver nanoparticles with special reference to antioxidant, antibacterial, hemolytic and in vivo cutaneous wound healing effects
Publication date: Available online 16 October 2018Source: Biochimica et Biophysica Acta (BBA) - General SubjectsAuthor(s): Sawssen Hajji, Sameh Ben Khedir, Ibtissem Hamza-Mnif, Marwa Hamdi, Ines Jedidi, Rim Kallel, Sami Boufi, Moncef NasriAbstractThe aim of the present study was to prepare chitosan-PVA‑silver nanoparticles (CS-AgNPs) through green method. Chitosan and PVA polymers acted as stabilizing agents. DLS and TEM analyses showed that CS-AgNPs were homogeneously dispersed in matrix with an average size of 190–200 nm. The CS-AgNPs were tested for their antioxidant and antibacterial properties and the result...
Source: Biochimica et Biophysica Acta (BBA) General Subjects - October 17, 2018 Category: Biochemistry Source Type: research

Catechin-Mediated Restructuring of a Bacterial Toxin Inhibits Activity
Conclusions: These results demonstrate that the catechins inhibit LtxA activity by altering its structure to prevent interaction with specific molecules present on the host cell surface. General Significance: Galloylated catechins modify protein toxin structure, inhibiting the toxin from binding to the requisite molecules on the host cell surface.Graphical abstract (Source: Biochimica et Biophysica Acta (BBA) General Subjects)
Source: Biochimica et Biophysica Acta (BBA) General Subjects - October 17, 2018 Category: Biochemistry Source Type: research

Interplay of Extracellular Vesicles and other players in Cerebral Malaria Pathogenesis
Conclusions.Microvesicles and exosomes are implicated in cerebral malaria pathogenesis, in the modulation of host immunity to Plasmodium, and in cell-cell communication. Blocking their production is protective in models of cerebral malaria, both in vivo and in vitro.General Significance.While anti-malarial treatments exist to combat Plasmodium infections, increasing drug resistance presents a major challenge. In order to improve diagnosis and treatment outcomes, further research is required to better appreciate extracellular vesicle involvement in cerebral malaria. (Source: Biochimica et Biophysica Acta (BBA) General Subjects)
Source: Biochimica et Biophysica Acta (BBA) General Subjects - October 17, 2018 Category: Biochemistry Source Type: research