Editorial Board
Publication date: October 2018Source: Biochimica et Biophysica Acta (BBA) - General Subjects, Volume 1862, Issue 10Author(s): (Source: Biochimica et Biophysica Acta (BBA) General Subjects)
Source: Biochimica et Biophysica Acta (BBA) General Subjects - August 15, 2018 Category: Biochemistry Source Type: research

Laying Down a Solid “Iron Foundation”: Professor Erica Baker
Publication date: October 2018Source: Biochimica et Biophysica Acta (BBA) - General Subjects, Volume 1862, Issue 10Author(s): Des R. Richardson (Source: Biochimica et Biophysica Acta (BBA) General Subjects)
Source: Biochimica et Biophysica Acta (BBA) General Subjects - August 15, 2018 Category: Biochemistry Source Type: research

Revealing the atomistic details behind the binding of B7–1 to CD28 and CTLA-4: A comprehensive protein-protein modelling study
ConclusionsPer-residue energy decomposition of the binding affinities of the two complexes revealed the unique fingerprint hot-spot sites in CTLA-4/B7–1 and CD28/B7–1 complexes.General Significance.The results presented in this work will, on a long-run, be useful to develop new generation of specific CD28 and CTLA-4 inhibitors for targeted immunotherapy. (Source: Biochimica et Biophysica Acta (BBA) General Subjects)
Source: Biochimica et Biophysica Acta (BBA) General Subjects - August 10, 2018 Category: Biochemistry Source Type: research

“Pinching” the Ammonia tunnel of CTP synthase unveils coordinated catalytic and allosteric-dependent control of Ammonia passage
Publication date: Available online 10 August 2018Source: Biochimica et Biophysica Acta (BBA) - General SubjectsAuthor(s): Gregory D. McCluskey, Stephen L. BearneAbstractMolecular gates within enzymes often play important roles in synchronizing catalytic events. We explored the role of a gate in cytidine-5′-triphosphate synthase (CTPS) from Escherichia coli. This glutamine amidotransferase catalyzes the biosynthesis of CTP from UTP using either l-glutamine or exogenous NH3 as a substrate. Glutamine is hydrolyzed in the glutaminase domain, with GTP acting as a positive allosteric effector, and the nascent NH3 passes th...
Source: Biochimica et Biophysica Acta (BBA) General Subjects - August 10, 2018 Category: Biochemistry Source Type: research

Lysine as a heme iron ligand: A property common to three truncated hemoglobins from Chlamydomonas reinhardtii
ConclusionsThree single-domain C. reinhardtii hemoglobins use lysine as a distal heme ligand in both Fe(III) and Fe(II) oxidation states. This common feature is likely related to enzymatic activity in the management of reactive oxygen species.General Significance.Primary structure analysis of hemoglobins has limited power in the prediction of heme ligation. Experimental determination reveals variations in this essential property across the superfamily.Graphical abstract (Source: Biochimica et Biophysica Acta (BBA) General Subjects)
Source: Biochimica et Biophysica Acta (BBA) General Subjects - August 10, 2018 Category: Biochemistry Source Type: research

Modulation of neurosecretion and approaches for its multistep analysis
ConclusionsOur algorithm enabled us to verify the method validity for multidimensional analysis of SV turnover. By increasing SV docking and the size of readily releasable pool (RRP), levetiracetam is able to selectively enhance the stimulated GABA secretion in hippocampal neurons. Findings suggest that SV2 regulates RRP through impact on the number of docked/primed SVs.General Significance.Methodology can be widely applied to study the stimulated neurosecretion in presynapse, regulation of SV docking, their Ca2+-dependent fusion with target membranes, quantitative analysis of expression of neuron-specific proteins, as wel...
Source: Biochimica et Biophysica Acta (BBA) General Subjects - August 7, 2018 Category: Biochemistry Source Type: research

Inhibition of amyloid fibril formation in the variable domain of λ6 light chain mutant Wil caused by the interaction between its unfolded state and epigallocatechin-3-O-gallate
ConclusionsInhibition of amyloid fibril formation of Wil was caused by 1) EGCG binding to unfolded state rather than folded state and 2) chemical modifications of Wil by auto oxidation of EGCG.General significanceIn the competitive formation of amyloid fibrils and off-pathway oligomers, EGCG produces the latter immediately after it preferentially binds to the unfolded state. It may be general mechanism of EGCG inhibition for amyloidosis.Graphical abstract (Source: Biochimica et Biophysica Acta (BBA) General Subjects)
Source: Biochimica et Biophysica Acta (BBA) General Subjects - August 7, 2018 Category: Biochemistry Source Type: research

Dynamics of DDB2-DDB1 complex under different naturally-occurring mutants in Xeroderma Pigmentosum disease
ConclusionsThe data gathered throughout the analyses helps enlighten the structural basis for how naturally occurring mutations found in XPE patients impact on DDB2 and DDB1 function.General significanceOur data influence not only on the knowledge of XP but on the DNA repair mechanisms of NER itself. (Source: Biochimica et Biophysica Acta (BBA) General Subjects)
Source: Biochimica et Biophysica Acta (BBA) General Subjects - August 7, 2018 Category: Biochemistry Source Type: research

Plasma protein N-glycan signatures of type 2 diabetes
ConclusionsWhile some glycosylation changes were reflective of inflammation, such as increased alpha2,6-linked sialylation, our finding of decreased alpha2,3-linked sialylation in type 2 diabetes patients is contradictory to reports on acute and chronic inflammation. Thus, it might have previously unreported immunological implications in type 2 diabetes.General significanceThis study provides new insights into N-glycosylation patterns in type 2 diabetes, which can fuel studies on causal mechanisms and consequences of this complex disease. (Source: Biochimica et Biophysica Acta (BBA) General Subjects)
Source: Biochimica et Biophysica Acta (BBA) General Subjects - August 7, 2018 Category: Biochemistry Source Type: research

Structural basis of phospholipase A2-like myotoxin inhibition by chicoric acid, a novel potent inhibitor of ophidian toxins
ConclusionsCA prevents the myotoxic activity of the toxin, preventing its activation by simultaneous binding with two critical regions.General significanceCA is a potential myotoxic inhibitor to other PLA2-like proteins and a possible candidate to complement serum therapy. (Source: Biochimica et Biophysica Acta (BBA) General Subjects)
Source: Biochimica et Biophysica Acta (BBA) General Subjects - August 4, 2018 Category: Biochemistry Source Type: research

LMW-PTP modulates glucose metabolism in cancer cells
ConclusionAll these findings propose that tumor cells are subjected to metabolic reprogramming after LMW-PTP silencing, enhancing glycolytic flux, probably to compensate the inhibition of mitochondrial metabolism.General significance.Our results highlight the involvement of LMW-PTP in regulating glucose metabolism in A375 melanoma cells. (Source: Biochimica et Biophysica Acta (BBA) General Subjects)
Source: Biochimica et Biophysica Acta (BBA) General Subjects - August 4, 2018 Category: Biochemistry Source Type: research

GAPDH with NAD+-binding site mutation competitively inhibits the wild-type and affects glucose metabolism in cancer
ConclusionMutant-GAPDH affects the enzymatic function of cellular-GAPDH and disrupts energy metabolism.General significanceOur findings demonstrate that a minimal mutation at the NAD+-binding site is sufficient to generate a competitive but dysfunctional GAPDH, and its ectopic expression inhibits the wild-type to disrupt glycolysis.Graphical abstract (Source: Biochimica et Biophysica Acta (BBA) General Subjects)
Source: Biochimica et Biophysica Acta (BBA) General Subjects - August 3, 2018 Category: Biochemistry Source Type: research

Transcriptome analyses reveal molecular mechanisms that regulate endochondral ossification in amphibian Bufo gargarizans during metamorphosis
ConclusionsThe exquisite coordination of the 28 genes is essential for regulation of endochondral ossification during B. gargarizans metamorphosis.General significanceThe present study will not only provide an invaluable genomic resource and background for further research of endochondral ossification in amphibians but will also aid in enhancing our understanding of the evolution of terrestrial vertebrates. (Source: Biochimica et Biophysica Acta (BBA) General Subjects)
Source: Biochimica et Biophysica Acta (BBA) General Subjects - August 2, 2018 Category: Biochemistry Source Type: research

Carbamylation promotes amyloidogenesis and induces structural changes in Tau-core hexapeptide fibrils
ConclusionOur in vitro and in silico results together suggest that carbamylation can modulate the aggregation pathway of the amyloidegenic sequences and cause structural changes in fibril assembliesGeneral significanceCarbamylation acts as a switch, which triggers the aggregation in short amyloidogenic peptide fragments and modulate the structural changes in resulting amyloid fibrils.Graphical abstract (Source: Biochimica et Biophysica Acta (BBA) General Subjects)
Source: Biochimica et Biophysica Acta (BBA) General Subjects - July 31, 2018 Category: Biochemistry Source Type: research

Electrostatic control of calcineurin's intrinsically-disordered regulatory domain binding to calmodulin
Publication date: Available online 31 July 2018Source: Biochimica et Biophysica Acta (BBA) - General SubjectsAuthor(s): Bin Sun, Erik C. Cook, Trever P. Creamer, Peter M. Kekenes-HuskeyAbstractCalcineurin (CaN) is a serine/threonine phosphatase that regulates a variety of physiological and pathophysiological processes in mammalian tissue. The calcineurin (CaN) regulatory domain (RD) is responsible for regulating the enzyme's phosphatase activity, and is believed to be highly-disordered when inhibiting CaN, but undergoes a disorder-to-order transition upon diffusion-limited binding with the regulatory protein calmodulin (Ca...
Source: Biochimica et Biophysica Acta (BBA) General Subjects - July 31, 2018 Category: Biochemistry Source Type: research

Thrombin binding aptamer analogues containing inversion of polarity sites endowed with antiproliferative and anti-motility properties against Calu-6 cells
ConclusionsBoth TBA analogues TBA353 and TBA535 are able to form G-quadruplex structures with no anticoagulant activity. However only TBA535 is endowed with noteworthy antiproliferative and anti-motility properties against lung cancer Calu-6 cells.General significanceThe switching from the anticoagulant to antiproliferative property can be obtained also in TBA derivatives not adopting the “chair-like” G-quadruplex structure typical of TBA. Furthermore, results have highlighted an unprecedented anti-cell-motility property of TBA and TBA535 reinforcing the potential of these ODNs as anticancer drugs.Graphical abs...
Source: Biochimica et Biophysica Acta (BBA) General Subjects - July 31, 2018 Category: Biochemistry Source Type: research

Recent developments in cell-SELEX technology for aptamer selection
Publication date: Available online 27 July 2018Source: Biochimica et Biophysica Acta (BBA) - General SubjectsAuthor(s): Harleen KaurAbstractBackgroundSELEX technique is employed to select aptamers against wide range of targets. The in vitro method of aptamer selection using live cells as the target is referred as cell-SELEX.Scope of the reviewThe review provides a comprehensive description on the development of aptamers through various cell-SELEX methods and list of aptamers identified through this method. In addition, it pinpoints the advantages and limitations of the cell-SELEX process and its variants.Major conclusionsT...
Source: Biochimica et Biophysica Acta (BBA) General Subjects - July 28, 2018 Category: Biochemistry Source Type: research

Molecular mechanism of Arabidopsis thaliana profilins as antifungal proteins
ConclusionPFNs may play a critical role as antifungal proteins in the Arabidopsis defense system against fungal pathogen attacks.General significanceThe present study indicates that two profilin proteins, AtPFN1 and AtPFN2, can act as natural antimicrobial agents in the plant defense system. (Source: Biochimica et Biophysica Acta (BBA) General Subjects)
Source: Biochimica et Biophysica Acta (BBA) General Subjects - July 26, 2018 Category: Biochemistry Source Type: research

Menadione sodium bisulfite inhibits the toxic aggregation of amyloid-β(1–42)
Publication date: October 2018Source: Biochimica et Biophysica Acta (BBA) - General Subjects, Volume 1862, Issue 10Author(s): Yu Zhang, Yudan Zhao, Zhuoyi Wang, Hao Gong, Liang Ma, Dongsheng Sun, Chen Yang, Yang Li, Biao Cheng, Robert B. Petersen, Fengchao Jiang, Gongping Liu, Kun HuangAbstractProtein misfolding and aggregation are associated with amyloidosis. The toxic aggregation of amyloid-β 1–42 (Aβ42) may disrupt cell membranes and lead to cell death and is thus regarded as a contributing factor in Alzheimer's disease (AD). 1,4-naphthoquinone (NQ) has been shown to exhibit strong anti-aggregation effec...
Source: Biochimica et Biophysica Acta (BBA) General Subjects - July 25, 2018 Category: Biochemistry Source Type: research

CaMKK2 kinase domain interacts with the autoinhibitory region through the N-terminal lobe including the RP insert
ConclusionsInteractions between the CaMKK2 kinase domain and the AID differ from those of other CaMKs. In the absence of Ca2+/CaM binding the autoinhibitory region inhibits CaMKK2 by both blocking access to the RP insert and by affecting the structure of the ATP-binding pocket.General significanceOur results corroborate the hypothesis that Ca2+/CaM binding causes unique conformational changes in the CaMKKs relative to other CaMKs.Graphical abstract (Source: Biochimica et Biophysica Acta (BBA) General Subjects)
Source: Biochimica et Biophysica Acta (BBA) General Subjects - July 25, 2018 Category: Biochemistry Source Type: research

Selenium research in biochemistry and biophysics - 200 year anniversary issue
Publication date: Available online 24 July 2018Source: Biochimica et Biophysica Acta (BBA) - General SubjectsAuthor(s): Elias S.J. Arnér, Regina Brigelius-Flohé (Source: Biochimica et Biophysica Acta (BBA) General Subjects)
Source: Biochimica et Biophysica Acta (BBA) General Subjects - July 25, 2018 Category: Biochemistry Source Type: research

Activation of membrane-bound and soluble Toll-like Receptors 5 in Salmo salar depends on the MyD88 signalling pathway
Publication date: October 2018Source: Biochimica et Biophysica Acta (BBA) - General Subjects, Volume 1862, Issue 10Author(s): C. Muñoz-Flores, A. Astuya, F.J. Roa, A. Romero, J. Acosta, O. Sánchez, J.R. Toledo (Source: Biochimica et Biophysica Acta (BBA) General Subjects)
Source: Biochimica et Biophysica Acta (BBA) General Subjects - July 24, 2018 Category: Biochemistry Source Type: research

Quantitation of NAD+: Why do we need to measure it?
Publication date: Available online 23 July 2018Source: Biochimica et Biophysica Acta (BBA) - General SubjectsAuthor(s): Yue Liu, James Clement, Ross Grant, Perminder Sachdev, Nady BraidyAbstractBackgroundNicotinamide adenine dinucleotide (NAD+) is an essential pyridine nucleotide that is currently investigated as an important target to extend lifespan and health span. Age-related NAD+ depletion due to the accumulation of oxidative stress is associated with reduced energy production, impaired DNA repair and genomic instability.Scope of reviewNAD+ levels can be elevated therapeutically using NAD+ precursors or through lifest...
Source: Biochimica et Biophysica Acta (BBA) General Subjects - July 24, 2018 Category: Biochemistry Source Type: research

Effect of amyloid beta on ATP-binding cassette transporter expression and activity in porcine brain microvascular endothelial cells
Publication date: Available online 23 July 2018Source: Biochimica et Biophysica Acta (BBA) - General SubjectsAuthor(s): Maryam H. Shubbar, Jeffrey I. PennyAbstractBackgroundDeposition of amyloid-β peptide (Aβ(1–42)) within the brain is characteristic of Alzheimer's disease. Little is known of the effects of Aβ(1–42) on blood-brain barrier (BBB) ATP-binding Cassette (ABC) efflux transporters which influence BBB permeability. The effects of Aβ(1–42) on ABCB1, ABCC5 and ABCG2 activity and expression and pregnane X receptor (PXR) and constitutive androstane receptor (CAR) transcription fac...
Source: Biochimica et Biophysica Acta (BBA) General Subjects - July 24, 2018 Category: Biochemistry Source Type: research

Structural Basis for the Hepatoprotective Effects of Antihypertensive 1,4-Dihydropyridine Drugs
ConclusionsThis study reports the crystal structures of FXR in complex with four DHPs, and confirms that DHPs exert hepatoprotection by targeting FXR.General Significance.Our research not only reveals valuable insight for the design and development of next-generation Ca2+ blocker drugs to provide safer and more effective treatments for cardiovascular disorders but also provides a novel and safe structural template for the development of drugs targeting FXR. Moreover, DHPs might be potentially repurposed to treat FXR-mediated diseases other than hypertension.Graphical abstract (Source: Biochimica et Biophysica Acta (BBA) General Subjects)
Source: Biochimica et Biophysica Acta (BBA) General Subjects - July 24, 2018 Category: Biochemistry Source Type: research

Targeting Helicobacter pylori urease activity and maturation: In-cell high-throughput approach for drug discovery
ConclusionsThe developed system allows the concomitant screening of a large number of drug candidates that interfere with the urease activity both at the level of the enzyme catalysis and maturation.General significance.As inhibition of urease has the potential of being a global antibacterial strategy for a large number of infections, this work paves the way for the development of new candidates for antibacterial drugs.Graphical abstract (Source: Biochimica et Biophysica Acta (BBA) General Subjects)
Source: Biochimica et Biophysica Acta (BBA) General Subjects - July 24, 2018 Category: Biochemistry Source Type: research

Probing the conformational switch of I-motif DNA using tunable resistive pulse sensing
Publication date: Available online 24 July 2018Source: Biochimica et Biophysica Acta (BBA) - General SubjectsAuthor(s): Jing Shi, Ming ZhouAbstractI-motif DNA, which can fold and unfold reversibly in various environments, plays a significant role in DNA nanotechnology and biological functions. Thus, it is of fundamental importance to identify the different conformations of i-motif DNA. Here, we demonstrate that distinct structures of i-motif DNA conjugated to polystyrene spheres can be distinguished through tunable resistive pulse sensing technique. When dispersed in acidic buffer, i-motif DNA coating on polystyrene sphere...
Source: Biochimica et Biophysica Acta (BBA) General Subjects - July 24, 2018 Category: Biochemistry Source Type: research

Proteomic investigation on bio-corona of functionalized multi­walled carbon nanotubes
Conclusionsthe evaluation of proteins primary structures, present in all bio-coronas, did not highlight any correlation between the chemical functionalization on MWCNTs and the content of acid, basic and hydrophobic amino acids. Despite this, many proteins of bio-corona, formed on all f-MWCNTs, were involved in the inhibitor activity of serine- or cysteine- endopeptidases, a molecular function completely unrevealed in the human plasma as control. Finally, the interaction with immune system's proteins and apolipoproteins has suggested a possible biocompatibility and a favored bio-distribution of tested f-MWCNTs.General sign...
Source: Biochimica et Biophysica Acta (BBA) General Subjects - July 24, 2018 Category: Biochemistry Source Type: research

A fast and straightforward procedure for vault nanoparticle purification and the characterization of its endocytic uptake
Conclusions: These results provide the first evidence for an intrinsic propensity of the vault complex to undergo endocytic uptake cultured eukaryotic cells.General significance.The newly developed purification procedure will greatly facilitate any investigation based on the use of the vault particle as a natural nanocarrier. Its clathrin-mediated endocytic uptake observed in normal and in some tumor cell lines sheds light on its physiological role.Graphical abstract (Source: Biochimica et Biophysica Acta (BBA) General Subjects)
Source: Biochimica et Biophysica Acta (BBA) General Subjects - July 21, 2018 Category: Biochemistry Source Type: research

Menadione sodium bisulfite inhibits the toxic aggregation of amyloid-β(1–42).
Publication date: Available online 20 July 2018Source: Biochimica et Biophysica Acta (BBA) - General SubjectsAuthor(s): Yu Zhang, Yudan Zhao, Zhuoyi Wang, Hao Gong, Liang Ma, Dongsheng Sun, Chen Yang, Yang Li, Biao Cheng, Robert B. Petersen, Fengchao Jiang, Gongping Liu, Kun HuangAbstractProtein misfolding and aggregation are associated with amyloidosis. The toxic aggregation of amyloid-β 1–42 (Aβ42) may disrupt cell membranes and lead to cell death and is thus regarded as a contributing factor in Alzheimer's disease (AD). 1,4-naphthoquinone (NQ) has been shown to exhibit strong anti-aggregation effects on ...
Source: Biochimica et Biophysica Acta (BBA) General Subjects - July 21, 2018 Category: Biochemistry Source Type: research

Metabolic reprogramming in colon cancer reversed by DHTS through regulating PTEN/AKT/HIF1α mediated signal pathway
Conclusions: DHTS reversed metabolic reprogramming in colon cancer through PTEN/AKT/HIF1α-mediated signal pathway.General Significance: The study is the first to report the reverse of metabolic reprogramming by DHTS in colon cancer. Meantime, SIRT3/PTEN/AKT/HIF1α mediated signal pathway plays a critical role during this process. (Source: Biochimica et Biophysica Acta (BBA) General Subjects)
Source: Biochimica et Biophysica Acta (BBA) General Subjects - July 21, 2018 Category: Biochemistry Source Type: research

Reengineering the ligand sensitivity of the broadly tuned human bitter taste receptor TAS2R14
ConclusionsSince numerous modifications of the TAS2R14 binding pocket resulted in improved responses to individual agonists, we conclude that this bitter taste receptor might represent a suitable template for the engineering of the agonist profile of a chemoreceptive receptor.General significanceThe detailed structure-function analysis of the highly promiscuous and widely expressed TAS2R14 suggests that this receptor must be considered as potentially frequent target for known and novel drugs including undesired off-effects.Graphical abstract (Source: Biochimica et Biophysica Acta (BBA) General Subjects)
Source: Biochimica et Biophysica Acta (BBA) General Subjects - July 19, 2018 Category: Biochemistry Source Type: research

β-TrCP-dependent degradation of ASK1 suppresses the induction of the apoptotic response by oxidative stress
Publication date: Available online 18 July 2018Source: Biochimica et Biophysica Acta (BBA) - General SubjectsAuthor(s): Ran Cheng, Kohsuke Takeda, Isao Naguro, Tomohisa Hatta, Shun-ichiro Iemura, Tohru Natsume, Hidenori Ichijo, Kazuki HattoriAbstractApoptosis signal-regulating kinase 1 (ASK1) is a key player in the homeostatic response of many organisms. Of the many functions of ASK1, it is most well-known for its ability to induce canonical caspase 3-dependent apoptosis through the MAPK pathways in response to reactive oxygen species (ROS). As ASK1 is a regulator of apoptosis, its proper regulation is critical for the wel...
Source: Biochimica et Biophysica Acta (BBA) General Subjects - July 19, 2018 Category: Biochemistry Source Type: research

Editorial Board
Publication date: September 2018Source: Biochimica et Biophysica Acta (BBA) - General Subjects, Volume 1862, Issue 9Author(s): (Source: Biochimica et Biophysica Acta (BBA) General Subjects)
Source: Biochimica et Biophysica Acta (BBA) General Subjects - July 19, 2018 Category: Biochemistry Source Type: research

Conformational response to charge clustering in synthetic intrinsically disordered proteins
ConclusionsCharge clustering promotes IDP compaction, but the extent of its effects depends on the sequence context. Proline residues seem to play a role contrasting compaction.General significanceThese results contribute to the identification of sequence determinants of IDP conformational properties. They may also serve as an asset for rational design of non-natural IDPs with tunable degree of compactness.Graphical abstract (Source: Biochimica et Biophysica Acta (BBA) General Subjects)
Source: Biochimica et Biophysica Acta (BBA) General Subjects - July 18, 2018 Category: Biochemistry Source Type: research

Transcriptional regulation of FeS biogenesis genes: A possible shield against arsenate toxicity activated by Yap1
Publication date: Available online 17 July 2018Source: Biochimica et Biophysica Acta (BBA) - General SubjectsAuthor(s): Sofia M. da Silva, Liliana Batista-Nascimento, Ana Gaspar-Cordeiro, Laurence Vernis, Catarina Pimentel, Claudina Rodrigues-PousadaAbstractIn the eukaryotic model yeast Saccharomyces cerevisiae, arsenic (As) detoxification is regulated by two transcriptional factors, Yap8 and Yap1. Yap8 specifically controls As extrusion from the cell, whether Yap1 avoids arsenic-induced oxidative damages. Accordingly, cells lacking both Yap1 and Yap8 are more sensitive to arsenate than cells lacking each regulator individ...
Source: Biochimica et Biophysica Acta (BBA) General Subjects - July 18, 2018 Category: Biochemistry Source Type: research

Function of C-terminal peptides on enzymatic and interfacial adsorption properties of lipase from Gibberella zeae
Publication date: Available online 17 July 2018Source: Biochimica et Biophysica Acta (BBA) - General SubjectsAuthor(s): Fanghua Wang, Hui Zhang, Anna Czarna, Wuchong Chen, Bo Yang, Yonghua WangAbstractBackgroundThe crystal structure of lipase from Gibberella zeae (GZEL) indicates that its C-terminal extension is composed of a loop and a α-helix. This structure is unique, possibly providing novel evidence on lipase mechanisms.MethodsTwo C-terminally truncated mutants (GZEL-Δ(α-helix) and GZEL-Δ(α-helix+loop)) were constructed. The role of these secondary structure segments on enzymatic activiti...
Source: Biochimica et Biophysica Acta (BBA) General Subjects - July 18, 2018 Category: Biochemistry Source Type: research

Cyclooxygenases and lipoxygenases are used by the fungus Podospora anserina to repel nematodes
Publication date: Available online 17 July 2018Source: Biochimica et Biophysica Acta (BBA) - General SubjectsAuthor(s): Roselyne Ferrari, Isabelle Lacaze, Pauline Le Faouder, Justine Bertrand-Michel, Camille Oger, Jean-Marie Galano, Thierry Durand, Stéphane Moularat, Laetitia Chan Ho Tong, Charlie Boucher, Jaafar Kilani, Yohann Petit, Océane Vanparis, César Trannoy, Sylvain Brun, Hervé Lalucque, Fabienne Malagnac, Philippe SilarAbstractOxylipins are secondary messengers used universally in the living world for communication and defense. The paradigm is that they are produced enzymatically for th...
Source: Biochimica et Biophysica Acta (BBA) General Subjects - July 18, 2018 Category: Biochemistry Source Type: research

The oligomeric assembly of the phosphodiesterase-5 is a mixture of dimers and tetramers: A putative role in the regulation of function
ConclusionsThis is the first time that structural studies on the full length protein evidenced the assembly of PDE5 in tetramers in addition to the expected dimers.General SignificanceThe assembly of PDE5 in tetramers in platelets, beside the dimers, opens the possibility to alternative assembly/allosteric regulation of this enzyme, as component of large signaling complexes, in all cellular districts in which PDE5 is present. (Source: Biochimica et Biophysica Acta (BBA) General Subjects)
Source: Biochimica et Biophysica Acta (BBA) General Subjects - July 18, 2018 Category: Biochemistry Source Type: research

Morphine, a potential inhibitor of myeloperoxidase activity
In this study, we assessed the effect of morphine on myeloperoxidase (MPO), a hemic enzyme from azurophilic granules of polymorphonuclear neutrophils involved in the production of cytotoxic and microbicidal reactive oxidants during inflammatory response. Specific immunological extraction followed by enzyme detection (SIEFED) and molecular modeling (docking) were performed to study the potential anti-catalytic action of morphine on MPO in comparison with the inhibitory effects of reference antioxidant molecules quercetin, gallic acid and ascorbic acid. The reducing action of morphine on the MPO peroxidase cycle has been inv...
Source: Biochimica et Biophysica Acta (BBA) General Subjects - July 11, 2018 Category: Biochemistry Source Type: research

Selenolanthionine is the major water-soluble selenium compound in the selenium tolerant plant Cardamine violifolia
Publication date: Available online 10 January 2018Source: Biochimica et Biophysica Acta (BBA) - General SubjectsAuthor(s): Eszter Borbála Both, Shuxun Shao, Jiqian Xiang, Zsuzsa Jókai, Hongqing Yin, Yafeng Liu, Anna Magyar, Mihály DernovicsAbstractBackgroundSelenium hyperaccumulation in plants often involves the synthesis of non-proteinaceous methylated selenoamino acids serving for the elimination of excess selenium from plant metabolism to protect plant homeostasis.MethodsOur study aimed at the identification of the main selenium species of the selenium hyperaccumulator plant Cardamine violifolia (Br...
Source: Biochimica et Biophysica Acta (BBA) General Subjects - July 11, 2018 Category: Biochemistry Source Type: research

Comparison of ATP sulfurylase 2 from selenium hyperaccumulator Stanleya pinnata and non-accumulator Stanleya elata reveals differential intracellular localization and enzyme activity levels
ConclusionsThe ATPS2 from S. pinnata differs from non-accumulator ATPS2 in its elevated expression and sub-cellular localization. It likely acts on both selente and sulfate substrates.General significanceThese observations shed new light on the role of ATPS2 in the evolution of Se hyperaccumulation in plants. This article is part of a Special Issue entitled Selenium research in biochemistry and biophysics - 200 year anniversary issue, edited by Dr. Elias Arnér and Dr. Regina Brigelius-Flohe. (Source: Biochimica et Biophysica Acta (BBA) General Subjects)
Source: Biochimica et Biophysica Acta (BBA) General Subjects - July 11, 2018 Category: Biochemistry Source Type: research

On elongation factor eEFSec, its role and mechanism during selenium incorporation into nascent selenoproteins
This article is part of a Special Issue entitled “Selenium research” in celebration of 200 years of selenium discovery, edited by Dr. Elias Arnér and Dr. Regina Brigelius-Flohe. (Source: Biochimica et Biophysica Acta (BBA) General Subjects)
Source: Biochimica et Biophysica Acta (BBA) General Subjects - July 11, 2018 Category: Biochemistry Source Type: research

Selenium-dependent gene expression in Methanococcus maripaludis: Involvement of the transcriptional regulator HrsM
ConclusionsHrsM exerts a key role in regulating expression of selenoprotein genes and their Cys-encoding isogenes in M. maripaludis in a selenium-dependent fashion. However, this activity is not achieved via autoregulation but probably by a mechanism, which modulates the DNA-binding of HrsM.General significanceAlthough LTTRs are abundant in Bacteria, HrsM represents only the second characterized member of this group in Archaea. (Source: Biochimica et Biophysica Acta (BBA) General Subjects)
Source: Biochimica et Biophysica Acta (BBA) General Subjects - July 11, 2018 Category: Biochemistry Source Type: research

Mechanisms of selenium hyperaccumulation in plants: A survey of molecular, biochemical and ecological cues
Publication date: Available online 4 April 2018Source: Biochimica et Biophysica Acta (BBA) - General SubjectsAuthor(s): Leonardo Warzea Lima, Elizabeth A.H. Pilon-Smits, Michela SchiavonAbstractBackgroundSelenium (Se) is a micronutrient required for many life forms, but toxic at higher concentration. Plants do not have a Se requirement, but can benefit from Se via enhanced antioxidant activity. Some plant species can accumulate Se to concentrations above 0.1% of dry weight and seem to possess mechanisms that distinguish Se from its analog sulfur (S). Research on these so-called Se hyperaccumulators aims to identify key gen...
Source: Biochimica et Biophysica Acta (BBA) General Subjects - July 11, 2018 Category: Biochemistry Source Type: research

Regulation and function of avian selenogenome
Publication date: Available online 5 April 2018Source: Biochimica et Biophysica Acta (BBA) - General SubjectsAuthor(s): Shuping Li, Fei Gao, Jiaqiang Huang, Yuanyuan Wu, Sen Wu, Xin Gen LeiAbstractBackgroundSelenium (Se) is an essential micronutrient required by avian species. Dietary Se/vitamin E deficiency induces three classical diseases in chicks: exudative diathesis, nutritional pancreatic atrophy, and nutritional muscular dystrophy.Scope of reviewThis review is to summarize and analyze the evolution, regulation, and function of avian selenogenome and selenoproteome and their relationship with the three classical Se/v...
Source: Biochimica et Biophysica Acta (BBA) General Subjects - July 11, 2018 Category: Biochemistry Source Type: research

Investigation into the intracellular fates, speciation and mode of action of selenium-containing neuroprotective agents using XAS and XFM
ConclusionsThe differences in activity were thus attributed to the varied speciation and cellular localisation of the compounds, or their metabolites, as detected by X-ray absorption spectroscopy and X-ray fluorescence microscopy.SignificanceThe work is significant as it links, for the first time, the protective action of selenium compounds against redox stress with particular chemical speciation using a direct measurement approach. (Source: Biochimica et Biophysica Acta (BBA) General Subjects)
Source: Biochimica et Biophysica Acta (BBA) General Subjects - July 11, 2018 Category: Biochemistry Source Type: research

Selenium supplementation prevents metabolic and transcriptomic responses to cadmium in mouse lung
ConclusionCo-administration of Se with Cd prevented Cd increase in lung and prevented Cd-associated pathway and network responses of the transcriptome and metabolome. Se protection against Cd toxicity in lung involves complex systems responses.General significanceEnvironmental Cd stimulates proinflammatory and profibrotic signaling. The present results indicate that dietary or supplemental Se could be useful to mitigate Cd toxicity. (Source: Biochimica et Biophysica Acta (BBA) General Subjects)
Source: Biochimica et Biophysica Acta (BBA) General Subjects - July 11, 2018 Category: Biochemistry Source Type: research

Molecular mechanism of selenoprotein P synthesis
Publication date: Available online 12 April 2018Source: Biochimica et Biophysica Acta (BBA) - General SubjectsAuthor(s): Sumangala Shetty, Paul R. CopelandAbstractBackgroundSelenoprotein synthesis requires the reinterpretation of a UGA stop codon as one that encodes selenocysteine (Sec), a process that requires a set of dedicated translation factors. Among the mammalian selenoproteins, Selenoprotein P (SELENOP) is unique as it contains a selenocysteine-rich domain that requires multiple Sec incorporation events.Scope of reviewIn this review we elaborate on new data and current models that provide insight into how SELENOP i...
Source: Biochimica et Biophysica Acta (BBA) General Subjects - July 11, 2018 Category: Biochemistry Source Type: research

Selenium-regulated hierarchy of human selenoproteome in cancerous and immortalized cells lines
ConclusionsWe characterized that selenoprotein hierarchy is cell-line specific with conserved features. This analysis should be done prior to any experiments in a novel cell line.General significanceWe reported a strategy based on complementary methods to evaluate selenoproteome regulation in human cells in culture. (Source: Biochimica et Biophysica Acta (BBA) General Subjects)
Source: Biochimica et Biophysica Acta (BBA) General Subjects - July 11, 2018 Category: Biochemistry Source Type: research