High-density bin-based genetic map reveals a 530-kb chromosome segment derived from wild peanut contributing to late leaf spot resistance
In this study, a synthetic amphidiploid (Ipadur) of wild peanuts with high LLS resistance was used to cross with Tifrunner to construct TI population. In total, 200 recombinant inbred lines were collected for whole-genome resequencing. A high-density bin-based genetic linkage map was constructed, which includes 4,809 bin markers with an average inter-bin distance of 0.43 cM. The recombination across cultivated and wild species was unevenly distributed, providing a novel recombination landscape for cultivated-wild Arachis species. Using phenotyping data collected across three environments, 28 QTLs for LLS disease resistance...
Source: TAG. Theoretical and Applied Genetics - March 5, 2024 Category: Genetics & Stem Cells Authors: Jiaowen Pan Xiaojie Li Chun Fu Jianxin Bian Zhenyu Wang Conghui Yu Xiaoqin Liu Guanghao Wang Ruizheng Tian Xiaofeng Song Changsheng Li Han Xia Shuzhen Zhao Lei Hou Meng Gao Hailing Zi David Bertioli Soraya Leal-Bertioli Manish K Pandey Xingjun Wang Chuanz Source Type: research
A Review of Shared Decision-Making, Published Protocols, and Post-desensitization Strategies in Oral Immunotherapy (OIT)
AbstractPurpose of ReviewThe aim of this review is to highlight key published oral immunotherapy (OIT) protocols and post-desensitization strategies for the major food allergens and to cover important concepts to consider when evaluating OIT for food-allergic patients. Shared decision-making should help identify patient and family values which will help influence the type of evidence-based protocol and maintenance strategy to use.Recent FindingsWith food OIT emerging as a treatment option, there is a pressing need for patients, physicians, and other providers to have a nuanced understanding of the management choices availa...
Source: Current Allergy and Asthma Reports - March 5, 2024 Category: Allergy & Immunology Source Type: research
Fatal food anaphylaxis in adults and children
AbstractAnaphylaxis is a life-threatening reaction characterized by the acute onset of symptoms involving different organ systems and requiring immediate medical intervention. The incidence of fatal food anaphylaxis is 0.03 to 0.3 million/people/year. Most fatal food-induced anaphylaxis occurs in the second and third decades of life. The identified risk factors include the delayed use of epinephrine, the presence of asthma, the use of recreational drugs (alcohol, nicotine, cannabis, etc.), and an upright position. In the United Kingdom (UK) and Canada, the reported leading causal foods are peanuts and tree nuts. In Italy, ...
Source: Italian Journal of Pediatrics - March 5, 2024 Category: Pediatrics Source Type: research
Designing future peanut: the power of genomics-assisted breeding
Theor Appl Genet. 2024 Mar 4;137(3):66. doi: 10.1007/s00122-024-04575-3.ABSTRACTIntegrating GAB methods with high-throughput phenotyping, genome editing, and speed breeding hold great potential in designing future smart peanut cultivars to meet market and food supply demands. Cultivated peanut (Arachis hypogaea L.), a legume crop greatly valued for its nourishing food, cooking oil, and fodder, is extensively grown worldwide. Despite decades of classical breeding efforts, the actual on-farm yield of peanut remains below its potential productivity due to the complicated interplay of genotype, environment, and management fact...
Source: TAG. Theoretical and Applied Genetics - March 4, 2024 Category: Genetics & Stem Cells Authors: Ali Raza Hua Chen Chong Zhang Yuhui Zhuang Yasir Sharif Tiecheng Cai Qiang Yang Pooja Soni Manish K Pandey Rajeev K Varshney Weijian Zhuang Source Type: research
Troublemakers
Cartootorial: Studies on desensitization using food oral immunotherapy (OIT) have shown benefit in children, but efficacy in adults remains uncertain. A 2017 meta-analysis showed no significant benefit in adults, but the adult population was too small for the authors to draw any firm conclusions in this cohort.1 The 2018 PALISADE trial on peanut OIT allowed 41 highly allergic adults to participate.2 They found no significant benefit in this population and provided no further analysis because their prespecified outcomes were limited to children. (Source: Annals of Allergy, Asthma and Immunology)
Source: Annals of Allergy, Asthma and Immunology - March 1, 2024 Category: Allergy & Immunology Authors: Erin L. Reigh Tags: Marginal Zone Source Type: research
Production of a Ric c3 hypo-allergen with no IgE binding or anaphylactogenic activity
This study aimed to determine the effect of mutations in Ric c3 amino acid residues known to affect IgE binding and allergy challenges. Based on the Ric c3 structure, B-cell epitopes, and amino acid involved in IgE binding, we produce recombinant mutant protein, mrRic c3, secreted from E. coli. Strategic glutamic acid residues in IgE-biding regions were changed by Leucine. The allergenicity of mrRic c3 was evaluated by determination of IgE, IgG1, and total IgG in immunized Balb/c mice and by degranulation assays of mast cells isolated from Wistar rats. The mrRic c3 presented a percentage of mast cell degranulation close to...
Source: Braz J Biol - February 29, 2024 Category: Biology Authors: M G B Bartholazzi T M Lodi E S Mello A O Carvalho B C B Beir ão O L T Machado Source Type: research
Production of a Ric c3 hypo-allergen with no IgE binding or anaphylactogenic activity
This study aimed to determine the effect of mutations in Ric c3 amino acid residues known to affect IgE binding and allergy challenges. Based on the Ric c3 structure, B-cell epitopes, and amino acid involved in IgE binding, we produce recombinant mutant protein, mrRic c3, secreted from E. coli. Strategic glutamic acid residues in IgE-biding regions were changed by Leucine. The allergenicity of mrRic c3 was evaluated by determination of IgE, IgG1, and total IgG in immunized Balb/c mice and by degranulation assays of mast cells isolated from Wistar rats. The mrRic c3 presented a percentage of mast cell degranulation close to...
Source: Braz J Biol - February 29, 2024 Category: Biology Authors: M G B Bartholazzi T M Lodi E S Mello A O Carvalho B C B Beir ão O L T Machado Source Type: research
Production of a Ric c3 hypo-allergen with no IgE binding or anaphylactogenic activity
This study aimed to determine the effect of mutations in Ric c3 amino acid residues known to affect IgE binding and allergy challenges. Based on the Ric c3 structure, B-cell epitopes, and amino acid involved in IgE binding, we produce recombinant mutant protein, mrRic c3, secreted from E. coli. Strategic glutamic acid residues in IgE-biding regions were changed by Leucine. The allergenicity of mrRic c3 was evaluated by determination of IgE, IgG1, and total IgG in immunized Balb/c mice and by degranulation assays of mast cells isolated from Wistar rats. The mrRic c3 presented a percentage of mast cell degranulation close to...
Source: Braz J Biol - February 29, 2024 Category: Biology Authors: M G B Bartholazzi T M Lodi E S Mello A O Carvalho B C B Beir ão O L T Machado Source Type: research
Production of a Ric c3 hypo-allergen with no IgE binding or anaphylactogenic activity
This study aimed to determine the effect of mutations in Ric c3 amino acid residues known to affect IgE binding and allergy challenges. Based on the Ric c3 structure, B-cell epitopes, and amino acid involved in IgE binding, we produce recombinant mutant protein, mrRic c3, secreted from E. coli. Strategic glutamic acid residues in IgE-biding regions were changed by Leucine. The allergenicity of mrRic c3 was evaluated by determination of IgE, IgG1, and total IgG in immunized Balb/c mice and by degranulation assays of mast cells isolated from Wistar rats. The mrRic c3 presented a percentage of mast cell degranulation close to...
Source: Braz J Biol - February 29, 2024 Category: Biology Authors: M G B Bartholazzi T M Lodi E S Mello A O Carvalho B C B Beir ão O L T Machado Source Type: research
Production of a Ric c3 hypo-allergen with no IgE binding or anaphylactogenic activity
This study aimed to determine the effect of mutations in Ric c3 amino acid residues known to affect IgE binding and allergy challenges. Based on the Ric c3 structure, B-cell epitopes, and amino acid involved in IgE binding, we produce recombinant mutant protein, mrRic c3, secreted from E. coli. Strategic glutamic acid residues in IgE-biding regions were changed by Leucine. The allergenicity of mrRic c3 was evaluated by determination of IgE, IgG1, and total IgG in immunized Balb/c mice and by degranulation assays of mast cells isolated from Wistar rats. The mrRic c3 presented a percentage of mast cell degranulation close to...
Source: Braz J Biol - February 29, 2024 Category: Biology Authors: M G B Bartholazzi T M Lodi E S Mello A O Carvalho B C B Beir ão O L T Machado Source Type: research
The Natural History and Risk Factors for the Development of Food Allergies in Children and Adults
AbstractPurpose of ReviewThis narrative review explores food allergy prevalence and natural history stratified by life stages, especially in context of evolving knowledge over the last few decades.Recent FindingsThe prevalence of food allergy remains highest in early childhood with common food triggers being cow ’s milk, soy, hen’s egg, wheat, peanut, tree nuts, sesame, fish, and shellfish. This correlates with certain risk factors especially pertinent in the postnatal period which appear to predispose an individual to developing a food allergy. Some allergies (such as milk and egg) were previously thou ght to be easil...
Source: Current Allergy and Asthma Reports - February 28, 2024 Category: Allergy & Immunology Source Type: research
Detection of two homologous major QTLs and development of diagnostic molecular markers for sucrose content in peanut
In this study, a high-density genetic map was constructed based on whole-genome re-sequencing data from a peanut RIL population. This map consisted of 2,042 bins and 24,142 SNP markers, making it one of the most comprehensive maps to date in terms of marker density. Two major QTLs (qSCA06.2 and qSCB06.2) were identified, explaining 31.41% and 24.13% of the phenotypic variance, respectively. Notably, these two QTLs were located in homologous genomic regions between the A and B subgenomes. The elite allele of qSCA06.2 was exclusive to Valencia-type, while the elite allele of qSCB06.2 existed in other peanut types. Importantl...
Source: TAG. Theoretical and Applied Genetics - February 27, 2024 Category: Genetics & Stem Cells Authors: Zhihui Wang Yue Zhang Dongxin Huai Yuning Chen Xin Wang Yanping Kang Liying Yan Huifang Jiang Kede Liu Yong Lei Boshou Liao Source Type: research
Detection of two homologous major QTLs and development of diagnostic molecular markers for sucrose content in peanut
In this study, a high-density genetic map was constructed based on whole-genome re-sequencing data from a peanut RIL population. This map consisted of 2,042 bins and 24,142 SNP markers, making it one of the most comprehensive maps to date in terms of marker density. Two major QTLs (qSCA06.2 and qSCB06.2) were identified, explaining 31.41% and 24.13% of the phenotypic variance, respectively. Notably, these two QTLs were located in homologous genomic regions between the A and B subgenomes. The elite allele of qSCA06.2 was exclusive to Valencia-type, while the elite allele of qSCB06.2 existed in other peanut types. Importantl...
Source: TAG. Theoretical and Applied Genetics - February 27, 2024 Category: Genetics & Stem Cells Authors: Zhihui Wang Yue Zhang Dongxin Huai Yuning Chen Xin Wang Yanping Kang Liying Yan Huifang Jiang Kede Liu Yong Lei Boshou Liao Source Type: research
Genes, Vol. 15, Pages 303: Autotetraploid Induction of Three A-Genome Wild Peanut Species, Arachis cardenasii, A. correntina, and A. diogoi
Hsuan Chen
A-genome Arachis species (AA; 2n = 2x = 20) are commonly used as secondary germplasm sources in cultivated peanut breeding, Arachis hypogaea L. (AABB; 2n = 4x = 40), for the introgression of various biotic and abiotic stress resistance genes. Genome doubling is critical to overcoming the hybridization barrier of infertility that arises from ploidy-level differences between wild germplasm and cultivated peanuts. To develop improved genome doubling methods, four trials of various concentrations of the mitotic inhibitor treatments colchicine, oryzalin, and trifluralin were tested on the seedlings and seeds of ...
Source: Genes - February 27, 2024 Category: Genetics & Stem Cells Authors: Robert W. Suppa Ryan J. Andres Jeffrey C. Dunne Ramsey F. Arram Thomas B. Morgan Hsuan Chen Tags: Article Source Type: research
Liamocins overproduction via the two-pH stage fermentation and anti-Aspergillus flavus activity of Massoia lactone
Biotechnol J. 2024 Feb;19(2):e2300675. doi: 10.1002/biot.202300675.ABSTRACTAureobasidium melanogenum was found to be grown the best at the constant pH 7.0 and to produce the highest amount of liamocins at the constant pH 3.0. Therefore, the wild type strain A. melanogenum 9-1 and the engineered strain V33 constructed in the laboratory were grown at the constant pH 7.0 for 48 h, then, they were continued to be cultivated at the constant pH 3.0. Under such conditions, A. melanogenum 9-1 produced 36.51 ± 0.55 g L-1 of liamocin and its cell mass was 27.43 ± 0.63 and 6.00 ± 0.11 g L-1 of glucose was left in the finished medi...
Source: Biotechnology Journal - February 26, 2024 Category: Biotechnology Authors: Peng Wang Mei Zhang Shou-Feng Zhao Zhao-Rui Zhang Guang-Lei Liu Zhe Chi Zhen-Ming Chi Source Type: research
