The Ratio Of Ara h 2-Specific IgE At 60 Months Of Age Relative To Earlier Samples May Be A Valuable Biomarker For Predicting Outcomes Of Oral Peanut Challenge At 60 months: Analysis Of Selected Samples From The LEAP Study.
There is need to develop simple approaches to predict the presence of clinically important allergy to peanuts without performing oral food challenges (OFC). This is particularly true for those with peanut specific IgE (sIgE)>0.1 or (Source: Journal of Allergy and Clinical Immunology)
Source: Journal of Allergy and Clinical Immunology - February 1, 2024 Category: Allergy & Immunology Authors: Joshua Bernstein, Carolyn Baloh, Gideon Lack, George Du Toit, Thao Vu, Stephen Dreskin Source Type: research
Prediction of peanut oral food challenge outcomes using machine learning
Food allergy is a severe public health problem. Food-specific skin and immunoglobulin E (IgE) tests provide limited diagnostic accuracy. Confirmatory oral food challenges (OFCs) are often required for diagnosis, but barriers hamper broad use. We utilized advanced machine learning methodologies to evaluate whether predictive models based on commonly-available clinical variables could outperform purely statistical methods for OFCs. (Source: Journal of Allergy and Clinical Immunology)
Source: Journal of Allergy and Clinical Immunology - February 1, 2024 Category: Allergy & Immunology Authors: Charles Schuler, Justin Zhang, Kayvan Najarian, Rajan Ravikumar, Georgiana Sanders, Jonathan Gryak Source Type: research
Analytical Performance of Peanut Component Allergens
Peanut allergen-specific IgE presents a serious anaphylactic risk for sensitized individuals and is one of the most researched serological markers for the diagnosis of peanut allergy. In an assay feasibility study, we evaluated the analytical performance of nAra h 1, rAra h 2, nAra h 3, nAra h 6, rAra h 8, and rAra h 9 on the IMMULITE ® 2000 XPi System. (Source: Journal of Allergy and Clinical Immunology)
Source: Journal of Allergy and Clinical Immunology - February 1, 2024 Category: Allergy & Immunology Authors: Tom Chuang, Cassandra Vaughn, Nicholas Ready, Meinir Rowlands, Jayne Chamberlain Source Type: research
INP20, a novel oral peanut immunotherapy that immunomodulates the immune system through Toll-like receptors
INP20 is a novel oral nanoparticle-based peanut immunotherapy with the potential to enhance the food oral immunotherapy effect acting on toll-like receptors (TLR). Among others, TLR2 and TLR4 activation increases IL-10 which promotes regulatory T responses. (Source: Journal of Allergy and Clinical Immunology)
Source: Journal of Allergy and Clinical Immunology - February 1, 2024 Category: Allergy & Immunology Authors: Marta Ferrer, Jorge Morales, Natalia Martinez de Baroja, Juan Manuel Irache, Maite Ag üeros, Marina Sabate Bresco, Jimena Crespo, Maria Jose Zavala, Carmen D, Evelyn Gutierrez, Maria José Goikoetxea, Blanca Esther García, Ana Isabel Tabar Purroy, Gabri Source Type: research
INP20, a peanut oral immunotherapy, is well tolerated by peanut-allergic patients in a double-blind placebo-controlled phase I clinical trial
INP20 is an oral nanoparticle-based peanut immunotherapy that is being tested in a double-blind placebo-controlled phase I clinical trial. The goal of this project is to evaluate the safety of this treatment in peanut-allergic patients. (Source: Journal of Allergy and Clinical Immunology)
Source: Journal of Allergy and Clinical Immunology - February 1, 2024 Category: Allergy & Immunology Authors: Marta Ferrer, Maria Jose Zavala, Jimena Crespo, Carmen D, Evelyn Gutierrez, Blanca Esther Garc ía, Gabriel Gastaminza, Sara Gomez, Natalia Martinez de Baroja, Jorge Morales, Maite Agüeros, Ana Isabel Tabar Purroy Source Type: research
A Streamlined Approach to Basophil Activation Testing for Longitudinal Characterization of Toddlers with Peanut Allergy During a Multi-Site Phase 3 Double-Blind Placebo-Controlled Trial of Epicutaneous Immunotherapy (EPIT)
The implementation of basophil activation testing (BAT) in multicenter studies remains complex. We recently developed a streamlined strategy for BAT (sBAT). Relying on ready-to-use reagents, it enables straightforward sample preparation at the clinical site before shipping to a central laboratory for analysis up to 5 days post-specimen collection. (Source: Journal of Allergy and Clinical Immunology)
Source: Journal of Allergy and Clinical Immunology - February 1, 2024 Category: Allergy & Immunology Authors: Alexander Grishin, Rihane Arif-Lusson, Penelope Bourgoin, Katie Matthews, Dianne Campbell, Henry Bahnson, Jean-Marc Busnel, Hugh Sampson Source Type: research
An Unintentional Randomized Trial of Early Environmental Exposure to Peanut: The Younger Siblings of LEAP Participants
The Dual Allergen Exposure Hypothesis proposes that food allergy develops due to cutaneous exposure in the absence of oral consumption. Evaluation of the younger siblings of the Learning Early About Peanut (LEAP) participants provides an opportunity to understand the impact of environmental exposure to peanut during infancy and early childhood on the development of peanut sensitization and allergy through a prospective randomized trial of high environmental versus low environmental peanut exposure. (Source: Journal of Allergy and Clinical Immunology)
Source: Journal of Allergy and Clinical Immunology - February 1, 2024 Category: Allergy & Immunology Authors: Gideon Lack, George Du Toit, Suzana Radulovic, Mary Feeney, Helen Fischer, Margie Byron, Lars Dunaway, Agustin Calatroni, Molly Johnson, Ru-Xin Foong, Andreina Marques-Mejias, Irene Bartha, Helen Brough, Monica Basting, Carolyn Baloh, Tanya Laidlaw, Henry Source Type: research
Spatiotemporal Immune Cells Profiling in Gastrointestinal Tissue Biopsies to Detect Oral Immunotherapy Induced Changes in Peanut Allergic Individuals
Oral immunotherapy (OIT) can treat the allergic reactions to a particular allergen in a food allergic individual. However, the OIT-induced changes involve complex interactions between the immune system, and the gastrointestinal system that are still unknown. We investigated gastrointestinal (GI) tract biopsies taken from our phase 2 peanut OIT trial- POISED using Multiplexed Ion Beam Imaging (MIBI) for spatiotemporal dynamics. (Source: Journal of Allergy and Clinical Immunology)
Source: Journal of Allergy and Clinical Immunology - February 1, 2024 Category: Allergy & Immunology Authors: Abhinav Kaushik, Roshan Angoshtari, Shirley Kwow, Neeraja Kambham, Nielsen Fernandez-Becker, Monali Manohar, Michael Angelo, Stephen Galli, Kari Nadeau, Rosemarie Dekruyff, Sharon Chinthrajah Source Type: research
Identification of QTLs associated with very-long chain fatty acid (VLCFA) content via linkage mapping and BSA-seq in peanut
This study initially revealed the genetic mechanism of VLCFA content, built a marker-assisted selection system for low VLCFA breeding, and provided an effective method to decrease the SFA content in peanut.PMID:38285195 | DOI:10.1007/s00122-024-04547-7 (Source: TAG. Theoretical and Applied Genetics)
Source: TAG. Theoretical and Applied Genetics - January 29, 2024 Category: Genetics & Stem Cells Authors: Xiaomeng Xue Jianguo Li Jie Wu Meiling Hu Nian Liu Liying Yan Yuning Chen Xin Wang Yanping Kang Zhihui Wang Huifang Jiang Yong Lei Chunyu Zhang Boshou Liao Dongxin Huai Source Type: research
From the Pages of AllergyWatch
For this edition of From Pages of AllergyWatch, I've selected articles from recent issues of AllergyWatch related to allergy prevention, the theme of this month's Annals. The first article, with comments from Dr. Joshi, reports a lack of causal data for PPIs increasing risk of childhood allergic disease. The next article, with comments from Dr. Otani, presents data for peanut consumption in pregnant and breastfeeding moms and the development of allergies in their infants. The third article, with comments from Dr. (Source: Annals of Allergy, Asthma and Immunology)
Source: Annals of Allergy, Asthma and Immunology - January 28, 2024 Category: Allergy & Immunology Authors: Stanley Fineman, Shyam R. Joshi, Iris Otani, Samantha Knox Tags: Special Series Source Type: research
Genes, Vol. 15, Pages 160: Genome-Wide Association Studies of Embryogenic Callus Induction Rate in Peanut (Arachis hypogaea L.)
In this study, to examine the genetic variation in embryogenic callus induction rate (CIR) in peanut (Arachis hypogaea L.) at the seventh, eighth, and ninth subcultures (T7, T8, and T9, respectively), we performed genome-wide association studies (GWAS) for CIR in a population of 353 peanut accessions. The coefficient of variation of CIR among the genotypes was high in the T7, T8, and T9 subcultures (33.06%, 34.18%, and 35.54%, respectively), and the average CIR ranged from 1.58 to 1.66. A total of 53 significant single-nucleotide polymorphisms (SNPs) were detected (based on the threshold value −log10(p) = 4.5...
Source: Genes - January 26, 2024 Category: Genetics & Stem Cells Authors: Dandan Luo Lei Shi Ziqi Sun Feiyan Qi Hongfei Liu Lulu Xue Xiaona Li Han Liu Pengyu Qu Huanhuan Zhao Xiaodong Dai Wenzhao Dong Zheng Zheng Bingyan Huang Liuyang Fu Xinyou Zhang Tags: Article Source Type: research
Serologic measurements for peanut allergy: Predicting clinical severity is complex
Ann Allergy Asthma Immunol. 2024 Jan 24:S1081-1206(24)00017-6. doi: 10.1016/j.anai.2024.01.018. Online ahead of print.ABSTRACTAllergist-immunologists use serologic peanut allergy testing to maximize test sensitivity and specificity while minimizing cost and inconvenience. Recent advances toward this goal include a better understanding of specific IgE (sIgE) and component testing, epitope-sIgE assays, and basophil activation testing. Predicting reaction severity with serologic testing is challenged by a range of co-factors that influence reaction severity, such as the amount and form of any allergen consumed and comorbid di...
Source: Annals of Allergy, Asthma and Immunology - January 25, 2024 Category: Allergy & Immunology Authors: Alexandra E Conway David B K Golden Helen A Brough Alexandra F Santos Marcus S Shaker Source Type: research
Biochanin A - A G6PD inhibitor: In silico and in vitro studies in non-small cell lung cancer cells (A549)
In conclusion, our findings highlight the potential of BA as a therapeutic agent in cancer treatment, specifically by targeting G6PD and related pathways. BA's varied effects, which range from anti-inflammatory capabilities to metastasis reduction, make it an appealing option for future investigation in the development of new cancer therapeutics.PMID:38266663 | DOI:10.1016/j.tiv.2024.105785 (Source: Toxicology in Vitro)
Source: Toxicology in Vitro - January 24, 2024 Category: Toxicology Authors: Anjali B Thakkar Ramalingam B Subramanian Sampark S Thakkar Vasudev R Thakkar Parth Thakor Source Type: research
Development of reverse transcription loop-mediated isothermal amplification (RT-LAMP) for rapid detection of viruses infecting patchouli (Pogostemon cablin)
In this study, a field-based isothermal technique called reverse transcription loop-mediated isothermal amplification (RT-LAMP) was established for an early and specific detection of BBWV-2 and PStV. The oligo primers were designed to target the coat protein genes of PStV and BBWV-2. The reaction conditions, such as temperature and time duration, were optimized to 65 °C for 60 min. The LAMP amplicons positive for PStV and BBWV-2 revealed characteristic ladder-type bands following agarose gel electrophoresis. Further, a colorimetric assay using a metal ion-based indicator (Hydroxy-naphthol blue, HNB) was conducted to visua...
Source: Archives of Microbiology - January 23, 2024 Category: Microbiology Authors: Humaira Bano Jawaid A Khan Source Type: research
Genes, Vol. 15, Pages 140: Genome-Wide Mapping of Quantitative Trait Loci for Yield-Attributing Traits of Peanut
This study provides understanding of the genetic factor governing peanut traits and valuable insights for future breeding efforts aimed at improving yield and quality. (Source: Genes)
Source: Genes - January 23, 2024 Category: Genetics & Stem Cells Authors: Pushpesh Joshi Pooja Soni Vinay Sharma Surendra S. Manohar Sampath Kumar Shailendra Sharma Janila Pasupuleti Vincent Vadez Rajeev K. Varshney Manish K. Pandey Naveen Puppala Tags: Article Source Type: research
