Save 100 USD on our hN2™ Primary Neurons
Potent, Pure and Easy to CultureAssay data continues to roll in. I am pleased by the high lot to lot consistencies of our hN2 Primary Human Neurons resulting in data reproducibility. Here're an examples of outgrowth/tox assays: In conjunction with Molecular Devices' ImageeXpress HCI platform, hN2 differentiated neuronal cells can be used to evaluate potential toxic effects of test compounds on nuerite outgrowth through visualizing cells stained with β-lll tubulin.Figure: Dose response curves for the effect of neurotoxic agents on neurite outgrowth. hN2™ cells were cultured in the presence of cytotoxic compounds for 72h,...
Source: Neuromics - June 30, 2015 Category: Neuroscience Tags: Culturing Human Neurons Protocol Cytotoxicity Assays hN2 Kits hN2 Neurons Cell Cultures Neurite outgrowth Neurotoxicity Source Type: news
Save 100 USD on Our New Cardiomyocytes
Potent, Pure and Easy to CultureI am pleased to announce the addition of Human Cardiomyocytes to our Stem and Cell Based Assay Solutions.Image: Human Cardiomyocyte culture.These cells are optimized to provide addition options for in-vitro testing of drug to drug candidate toxicities allowing researchers to rule out the ineffective and potentially toxic small molecules/compounds early in the process.I want to make it easy for you to buy and try. If you are not delighted with your results, I will replace free of charge or refund your purchase. Please also note the select positive feedback on our other primary and stem cells:...
Source: Neuromics - June 24, 2015 Category: Neuroscience Tags: cardiomyocyte based assays cardiomyocytes Cell based assays Cytotoxicity Assays Drug Discovery Human Mesenchymal Stem Cells Source Type: news
Voices of our Customers
Your Feedback Matters-A lotOur follow up processes include actively engaging users of our solutions for feedback and input. This includes Rose Ludescher, Manager of Customer Satisfaction, calling or sending e-mails to each user within 3 weeks of shipment. At the core is our product rating website. If users identify products related issues, we replace them or refund their purchases.We want to hear from you...the good, the bad and the ugly. We welcome opportunities to fix issues.We are always pleased when customers share data with us. Here's a recent example.Image: Mouse striatum stained with D2 cell marker Enkephalin (RA141...
Source: Neuromics - June 22, 2015 Category: Neuroscience Tags: Customer Service Neuromics Neuropathy Nociception Pain Research Source Type: news
Network vs Isolated Bursting in Motor Neurons
Motor Neurons and MEA Dysregulated bursting is at the root of many motor neuron/neuromuscular junction disease. ArunA Biomedical teaming with Axion Biosystems have generated relevant bursting data from our Mouse Motor Neurons cultured on Axion-Bioystem's Maestro MEA.Figure: Mouse Motor Neuron Network Modulation by Bicuculline-ckeck out the entire presentation to learn more: GFP+ Motor Neurons: Development and in-vitro Functional Assessment on Microelectrode Arrays Protocol User's Guide for Culturing Motor Neuron on MEA(pdf - 679Kb)ArunA Biomedical's/Neuromic's Mouse Motor Neurons on Axion Biosystem's MEASave on Mouse Mot...
Source: Neuromics - June 15, 2015 Category: Neuroscience Tags: Bursting Assay GFP labeled mouse motor neurons MEA Microelectrode Arrays Neural Networks Neuronal Networks Synchronized Bursting Source Type: news
MEA and Motor Neurons
Plating Densities of Motor Neurons Matter!I wanted to share some of the tips and data shared during the ArunA Biomedical's/Axion Biosystem's Webinar on MEA and our Mouse Motor NeuronsPlating Cells on Axion's MEASurface Coating PEI-laminin for adhesion and uniform monolayer development Dotting Constrains cells to the array Requires fewer cells per well Media changes every 2-3 daysVariations on Cell DensityDetailed Protocol for Culturing Motor Neurons on MEA Different Plating DensitiesPlating Densities between 60,000-80,000 OptimalAt these densities there was the least variation in mean firing rates. This data...
Source: Neuromics - June 10, 2015 Category: Neuroscience Tags: ALS Axion Bioystems Firing Rates GFP labeled mouse motor neurons MEA Microelectrode Arrays motor neuron diseases Plating Densities Source Type: news
Neuromics' Neurononal Cultures in Action
Drug Discovery and Tox Assay PublicationsOur Primary Human, Rat and Mouse Neurons are widely used and frequently cited in publications. Here's a pub hot of the presses referencing use of our e18 Rat Cortical Neurons to study the EPO-like cytoprotective effects cultures of these cells: James L. Miller, Timothy J. Church, Dmitri Leonoudakis, Karen Lariosa-Willingham, Normand L. Frigon, Connie S, Tettenborn, Jeffrey R. Spencer, and Juha Punnonen. Discovery and Characterization of Nonpeptidyl Agonists of The Tissue-Protective Erythropoietin Receptor. Molecular Pharmacology. May 27, 2015 mol.115.098400...Cells were is...
Source: Neuromics - June 3, 2015 Category: Neuroscience Tags: e-18 Primary Mouse Neurons e-18 Primary Rat Cortical Neurons EPO hN2 Neuron Primary Human Neurons primary Neuronal Cells s Cell Cultures Source Type: news
Neuromics' Stem Cell Markers
Potent, Proven and PublishedWe have a growing parade of Publications referencing use of our Stem Cell Markers. They have proven to be excellent for assays using our hNP1™ Human Neural Progenitor Kits and Human Mesenchymal Stem Cells (hMSCs),Here's a recent reference highlighting use of our Nucleostemin Antibody: Zhe Ding and He Huang. Mesenchymal stem cells in rabbit meniscus and bone marrow exhibit a similar feature but a heterogeneous multi-differentiation potential: superiority of meniscus as a cell source for meniscus repair. BMC Musculoskeletal Disorders (2015) 16:65 DOI 10.1186/s12891-015-0511-8. ...After removing ...
Source: Neuromics - May 29, 2015 Category: Neuroscience Tags: BMSCs Bone marrow derived stem cells meniscus-derived mesenchymal stem cells MMSCs Nucleostemin Nucleostemin Antibody Stem Cell Antibodies Stem cell markers Source Type: news
GFP Labeled Motor Neurons and MEA
Big Upcoming WebinarWe have been strategically partnering with ArunA Biomedical to improve how we serve Neuro-drug discovery and Neurotox research community. I am especially excited about how well are our GFP Labeled Mouse Neurons are working in many different and unique research applications. They have proved an important tool in the study of neuro-muscular diseases like ALS, Parkinson's and Multiple Sclerosis.I am pleased to announce a coming Webinar: “GFP+ Motor Neurons: Development and in-vitro Functional Assessment” Wednesday June 10th, 11:30 AM EDT-Register Today!Download Flyer. I will continue to post unique ass...
Source: Neuromics - May 21, 2015 Category: Neuroscience Tags: ALS Astrocyte-Glial-Neuron Co-cultures GFP labeled mouse motor neurons human Astrocytes MEA motor neuron diseases Multiple Sclerosis Parkinson ' s Disease Source Type: news
Proven Stem Cell Markers and Multiple Applications
More Bang for Your Buck!Our Stem Cell Markers remain a growth engine for Neuromics. One of the reasons that you would want to check out offerings are that we have selected markers that can be used in multiple applications.For example, many of our markers can be used for immunostaining assays to confirm your stem cells are expressing the correct proteins and then these calls can further characterized and purified by Flow Cytometry, Here's an example of one of our Nestins being used for these two applications.Figures: (A): Nestin is present in both cell lines, . (B) Corresponding validation by immunofluorescence analys...
Source: Neuromics - May 17, 2015 Category: Neuroscience Tags: Flow cytometry immunoblotting Immunocytochemistry Immunohistochemistry Immunostaining nestin nestin antibodies nestin antibody Neural Stem Cell Markers Source Type: news
3-D Screens Predict Breast Cancer Metastasis
Cell-ECM Interactions3-D culturing solutions and methods are increasingly being used to created more in vivo like assays.Here researchers use our Human Collagen IV extracellular matrix protein (ECM) to develop a simple biomaterial platform with systematic control over the ECM protein density and composition to determine if integrin binding governs how metastatic cells differentiate between secondary tissue sites: L.E. Barney, E.C. Dandley, L.E. Jansen, N.G. Reich, A.M. Mercurio, and S.R. Peyton. A cell–ECM screening method to predict breast cancer metastasis. Integr Biol (Camb). 2015 Feb 10; 7(2): 198–212. doi: 10...
Source: Neuromics - May 4, 2015 Category: Neuroscience Tags: 3D collagen scaffolds breast cancer ECMs Extracellular Matrix metastasis Source Type: news
Bioactive FGF Basic Recombinant Protein in Action
Maintaining Sheep Mesenchymal Stem Cell MultipotencyWe have been very selective in the Bioactive FGF2 or FGF-basic Recombinant Proteins we make available to Stem Cell Researchers. They are many options available so we are always encouraged when we see ours referenced in publications.Here researchers used our FGF2 to expand Sheep Bone Marrow Stem Cells (BMSCs) in culture: Troy D Bornes, Nadr M Jomha, Aillette Mulet-Sierra and Adetola B Adesida. Hypoxic culture of bone marrow-derived mesenchymal stromal stem cells differentially enhances in vitro chondrogenesis within cell-seeded collagen and hyaluronic acid porous scaffolds...
Source: Neuromics - April 27, 2015 Category: Neuroscience Tags: Chrondrocytes FGF Basic FGF Basic Recombinant Protein Fibroblast Growth Factor Basic Human Mesenchymal Stem Cells MSCs Source Type: news
Markers for Oligodendrocyte Progenitor Cells (OPCs)
Improving OPC ExpansionThe data provided in this publication demonstrates that the OPC yield from SVZ-derived cell cultures can be improved with the PDGF-BB isoform in comparison to classical bFGF-EGF, or PDGF-AA-based protocols. Additionally, it would be expected that the OPC-enriched cultures obtained from NSC/NPC exposure to PDGF-BB and heparin generate cells suitable for cell transplantation for treating demyelinating diseases. (Source: Neuromics)
Source: Neuromics - April 14, 2015 Category: Neuroscience Source Type: news
The Importance of in vivo Like Astroglial-Neuron Co-Cultures
What We Have LearnedI have many posting on both Neuron and Astroglial Cultures. We have now evolved our solution set to include in vivo like co-cultures. These Astroglial Neuron Co-cultures are designed to mimic in vivo like behaviors. They are potent, pure and proven to work in our clients' unique neurodegenerative disease and toxicity drug discovery assays.Why is this important? The mix of Astrocytes, Glia and Neurons in your co-cultures can impact your data endpoints and lead to inaccurate conclusions. Here're examples as to how much your data can fluctuate. These are data generated from toxicity assays.We stand re...
Source: Neuromics - April 6, 2015 Category: Neuroscience Tags: astrocyte cultures Astrocyte-Glial-Neuron Co-cultures Astrocytes Bis-1 hN2 Neurons Cell Cultures hNP1 Neural Progenitors Neurotoxicity Source Type: news
Nestin Expression in Differentiating Neural Progenitors
Breathtaking ImagesOur Nestin Antibody + a HES5::eGFP reporter was one of the markers used to visualize differentiating stem/progenitor cells (see: Nature Communications 6, Article number: 6500 doi:10.1038/ncomms7500). Figures: (a) Neural differentiation scheme. Neural induction was performed by a dual SMAD inhibition protocol followed by long-term propagation with the factors indicated for 220 days. Naming conventions representing neuroepithelial (NE), early radial glial (E-RG), midradial glial (M-RG), late radial glial (L-RG) and long-term cultured progenitors (LNP) are indicated. Number of passages are indicated as...
Source: Neuromics - March 28, 2015 Category: Neuroscience Tags: Human Neural Progenitors nestin nestin antibodies nestin antibody neural markers neural progenitors assays Source Type: news
Neuro-Toxicity Co-Culture
Poster Presentation at Society of Toxicologist Annual Meeting 2015.As part of Neuromics' strategic selling partnership with ArunA Biomedical. We are attending the SOT 2015 meeting in San Diego, CA.I am particular excited about our getting the word out on our solutions via poster presentations. Here's the line up:“In-vitro Human Developmental Neurotoxicity Screening Using Multiple Cell Types"Anirban Majumder1, Xian Wu2,3, Shelley Wallace1, Jane Le1, Steven L. Stice1,2,3 1ArunA Biomedical, Inc. Athens, GA, USA, 2Regenerative Bioscience Center, 3Interdisciplinary Toxicology Program, University of Georgia, Ath...
Source: Neuromics - March 23, 2015 Category: Neuroscience Tags: Aruna Biomedical Astrocyte-Glial-Neuron Co-cultures culturing hNP1 Neural Progenitors GFP labeled mouse motor neurons hN2 Neurons Cell Cultures Source Type: news
