Cancer-Associated Fibroblasts and Cancer Cell Migration
Highway for Cancer MetastasisCancer Associated Fibroblasts provide the Matrices  (ECMs) serve as cancer’s assistants as it spreads throughout the body.Cancer cells (red) migrate on a CAF-derived extracellular matrix (green). VANDERBILT UNIVERSITY; BEGUM ERDOGAN, DONNA WEBBUnlike other ECMs, which normally form dense meshes, these arrange into parallel bundles forming the highway for tumor cell migration (The Scientist-December 15, 2015). (Source: Neuromics)
Source: Neuromics - May 13, 2016 Category: Neuroscience Tags: CAFs cancer Cancer Associated Fibroblasts cancer biology cancer-associated fibroblasts cell migration metastasis Source Type: news

New Neuronal Astroglial PNS Markers
Save 70 USD on High Titer AntibodiesThe foundation of our company is built on our catalog of proven, published and high titer Neuron-Astroglia and PNS Markers. We are pleased to offer 75 USD off our latest additions. Antibody Type Species Reactivity Applications Aldolase-C Calbindin Calretinin Fox3/NeuN MAP2 Myelin Basic Protein Neurofilament NF-H Neurofilament NF-M Parvalbumin Secretagogin VSNL1 Mouse IgG Chicken IgY Chicken IgY Mouse IgG Mouse IgG Mouse IgG Mouse IgG Mouse IgG Chicken IgY Rabbit IgG Mouse IgG B; H; M; R B; H...
Source: Neuromics - May 2, 2016 Category: Neuroscience Tags: Astrocyte marker antibody Astrocyte Markers Astrocyte-Glial-Neuron Co-cultures glial cell Glial Markers Muscle Nerve junction neural markers Neuronal Markers Source Type: news

ELISA Blocking Buffers
Paramount to Reducing Background Noise and Attaining an Accurate SignalOnce the antibody or protein antigens have been properly adsorbed onto the ELISA/EIA plate, the next critical step in creating a reliable immunoassay is the blocking of the plate. This key is:Preventing nonspecific binding Reducing ELISA background signal Blocking nonspecific binding to adsorbed proteins Stabilizing proteins adsorbed to plate for better interactions Neuromics's ELISA Blocking Buffer formulations reduce nonspecific binding of sample and assay components to the ELISA well while stabilizing th...
Source: Neuromics - April 27, 2016 Category: Neuroscience Tags: Angiogenesis Antibody Arrays ELISA Blocking Buffers ELISA Techniques sandwich ELISA Source Type: news

Isolating and Selecting T-Cells
Save 100 USD on New KitsWe are pleased to announce the addition of our New T-Cell Isolation/Selection Kits. They are fast and easy. Check out the Short Protocol.Figure CD4+ T-Cell Isolation Process doi:10.1371/journal.pone.0035798.We also have T-Cell Expansion Kits!NameCatalog #TypeSpeciesApplicationsSizePriceStrepMan Magnet for 15ml and 50ml Tubes6-5650-065Cell Isolation-SeparationHCell Assays1 Magnet$400CD3 Fab Streptamer Isolation Kit MB6-8000-201Cell Isolation-SeparationH1 Kit$699CD4 Fab Streptamer Isolation Kit MB6-8000-206Cell Isolation-SeparationH1 Kit$699CD8 Fab Streptamer Isolation Kit MB6-8000-203Cell Isolation-S...
Source: Neuromics - April 15, 2016 Category: Neuroscience Tags: Cancer Research CD4 Cells CD8 Cells immunology T-Cell Expansion T-Cell Isolation Source Type: news

Magic Red and Cancer Research
Measure Apoptosis in Real Time in Cancer CellsMagic Red™ (MR) Kits measure apoptosis via active caspases and cathepsins in whole living, intact cells - no lysis required. As apoptosis progresses and caspase activity increases, the red fluorescent signal increases.In this example, Magic Red is used to measure apoptosis in HeLa Cells after treatment with Artesunate (ART), an anti-malarial drug and potential chemotherapy agent. Figure: ART activates lysosomal function. A, HeLa cells were first treated with ART (50 μM) for 12 h, followed by LTR labeling for 30 min, and further analyzed using confocal microscopy. B, He...
Source: Neuromics - April 2, 2016 Category: Neuroscience Tags: chemotherapies chemotherapy HeLA Cells live cell imaging Magic Red Magic Red Apoptosis Kit Source Type: news

Human Astrocytes
Cortex Derived AstrocytesNeuromics is pleased to be offering yet another option for culturing Human Astrocytes. They can be passaged up to 10X-Only 749 USD/500,000 CellsThey are deigned to be easy to culture and grow. Image: Human Brain Astrocytes cultured with our AlphaBioCoat.Check out our large offering of Neurons, Astroglia, Progenitors, Brain Endothelial Cells/Pericytes and Blood Brain Barrier (BBB) Model. Should you have questions on these or any of our offerings, I can be reached directly at 612-801-1007 or pshuster@neuromics.com. Thank you, Pete Shuster-CEO and Owner. (Source: Neuromics)
Source: Neuromics - March 31, 2016 Category: Neuroscience Tags: BBB Model Blood Brain Barrier Models human Astrocytes human brain microvascular endothelial cells Human Neural Progenitors Neurons Pericytes Source Type: news

Epigenetics and Pain Research
i-Fect Used to Study ImpactsOur i-Fect siRNA, miRNA and shRNA Trasfection Kit was recently used to study the impact of G9a-specific siRNA (AGUAACGGGCAUCAAUGC) on Mu Opioid Receptors: Yuhao Zhang, Shao-Rui Chen, Geoffroy Laumet, Hong Chen and Hui-Lin Pan. Nerve Injury Diminishes Opioid Analgesia through Lysine Methyltransferase-Mediated Transcriptional Repression of µ-Opioid Receptors in Primary Sensory Neurons. First Published on February 25, 2016, doi: 10.1074/jbc.M115.711812... In some SNL rats, G9a-specific siRNA (4 µg) or the negative control siRNA was administered intrathecally. G9a-specific siRNA(AGU...
Source: Neuromics - March 24, 2016 Category: Neuroscience Tags: DOR Dorsal Root Ganglia Ga9 MOR inhibitor i-Fect Morphine Mu Opioid Receptor Neuropathic Pain Source Type: news

3-D Tissue Models Made Easy
3 Steps to an in vivo Like AssayWe provide both CollaGel Hydrogels with different stiffness options and ready made 3-D Human Tissue Models. Building these models can be done in 3 easy steps:Step 1: Thaw our CollaGel Hydrogel solution Step 2: Add your cell suspensions Step 3: Watch it gelHUVECS intergrated into 3-D CollaGel environment.Due to the way in which it gels, our Collagel Hydrogel is well suited to Bio Printing models.I will be posting more data as it becomes available. (Source: Neuromics)
Source: Neuromics - March 15, 2016 Category: Neuroscience Tags: 3-D Cell based Assays 3-D Tissue Modles 3D Cell Based Assays. 3D collagen scaffolds Collagel human umbilical cord endothelial cells Source Type: news

CRISPR Everywhere
Nature Explores What it MeansHere's the link: http://www.nature.com/news/crispr-everywhere-1.19511We are part of the CRISPR everywhere revolution!Features Include:Simple-"Cut and Paste" Integration and Editing. Check out our Sleeping Beauty Transposon System Users Manual Potent-100% Stable with No Off target effects with the safest transposon insertion profile. Fast- Transfect with our transposase + Sleeping Beauty TransposonTM Reporter Series Vectors. Cost Effective- Starting at $329 (Source: Neuromics)
Source: Neuromics - March 12, 2016 Category: Neuroscience Tags: CRISPR CRISPR-Cas9 gene editing Plasmid transposase transposons Source Type: news

CRISP Everywhere
Nature Explores What it MeansHere's the link: http://www.nature.com/news/crispr-everywhere-1.19511We are part of the CRISPR everywhere revolution!Features Include:Simple-"Cut and Paste" Integration and Editing. Check out our Sleeping Beauty Transposon System Users Manual Potent-100% Stable with No Off target effects with the safest transposon insertion profile. Fast- Transfect with our transposase + Sleeping Beauty TransposonTM Reporter Series Vectors. Cost Effective- Starting at $329 (Source: Neuromics)
Source: Neuromics - March 12, 2016 Category: Neuroscience Tags: CRISPR CRISPR-Cas9 gene editing Plasmid transposase transposons Source Type: news

Check Out Our 3D Human Skin Tissue Model
In vivo Like Model for Studying Skin Diseases/IrritantsI am pleased to announce this new addition to our Cell Based Assay offerings.3D Human Skin Tissue ModelCross section of Human Skin ModelThe model includes precultured tissue in our engineered and tissue/cell specific Collagel-Hydrogels.Our 3D human skin model is manufactured in a GMP lab. The model is engineered from multilayered, differentiated epidermal cells. The tissue is provided cultured in inserts using serum free media. The model mimics human skin. It provides an in vivo like environment for studying dermal related diseases and cancers. It is also excellent for...
Source: Neuromics - March 4, 2016 Category: Neuroscience Tags: 3-D Angiogenesis Model 3-D Cell Culturing 3D Cell Based Assays. 3D collagen scaffolds 3D Human Skin Tissue Model BBB Model Blood Brain Barrier Models Skin Cancer Source Type: news

Stem Cell " Stemness " and Differentiation States
Markers Play a Key Role Our Stem Cell Markers are widely used and frequently published. These play an important role for understanding the molecular events that take place during the differentiation of human pluripotent cells. They are essential for the protocols aimed at insuring the generation high of quality differentiated cells. Here's an excellent study on the determining the state of differentiating stem cells: Julio Casta ño, Cristina Morera, Borja Sesé, Stephanie Boue, Carles Bonet-Costa, Merce Martí, Alicia Roque, Albert Jordan, Maria J. Barrero. SETD7 Regulates the Differentiation of Hu...
Source: Neuromics - February 22, 2016 Category: Neuroscience Tags: adult stem cells embryonic stem cells Stem Cell Differentiation Stem Cell Differentiation Protocol Source Type: news

Stem Cell "Stemness" and Differentiation States
Markers Play a Key RoleOur Stem Cell Markers are widely used and frequently published. These play an important role for understanding the molecular events that take place during the differentiation of human pluripotent cells. They are essential for the protocols aimed at insuring the generation high quality differentiated cells.Here's an excellent study on the determining the state of differentiating stem cells: Julio Castaño, Cristina Morera, Borja Sesé, Stephanie Boue, Carles Bonet-Costa, Merce Martí, Alicia Roque, Albert Jordan, Maria J. Barrero. SETD7 Regulates the Differentiation of Human Embryoni...
Source: Neuromics - February 22, 2016 Category: Neuroscience Tags: adult stem cells embryonic stem cells Stem Cell Differentiation Stem Cell Differentiation Protocol Source Type: news

3-D Cell Based Assays Are Evolving
3-D Multicell Models and GelsThere is a growing body of publications of why 3-D matters in drug discovery and toxicology assays. This table shows important distinctions for single cell assays. Cellular characteristics 2D 3D Refs. Morphology Sheet-like flat and stretched cells in monolayer Natural shape in spheroid/aggregate structures 20,24,50 Proliferation Often proliferate at a faster rate than in vivo May proliferate at a faster/slower rate compared to 2D-cultured cells depending on cell type and/or type of 3D model system 17,51 Exposure to medium/drugs Cells in monolayer are equally exposed ...
Source: Neuromics - February 15, 2016 Category: Neuroscience Tags: 3-D Assays. 3-D imaging 3-D Cell based Assays Astrocytes BBB Model Blood Brain Barrier Models brain microvascular endothelial cells Pericytes Source Type: news

Ready to Use Blood Brain Barrier Model (BBB)
6, 12 and 24 Well OptionsWe are pleased to introduce our BBB Model. It is designed to provide more in vivo like data for your drug discovery and toxicology assays.Our model mimic transport properties of the BBB due to the formation of tight junctions, higher expression of specific carriers, or great cell viability. The model includes brain endothelial cells with pericytes and astrocytes layered in an insert. This model improves endothelial cell polarization and enhance the formation of tight junctions, provide better endothelial cell-to-cell contact that is important for barrier development, and prevent the dilution of sec...
Source: Neuromics - February 9, 2016 Category: Neuroscience Tags: 3-D collagen scaffolds 3D Cell Based Assays. BBB < Blood Brain Barrier Model drug permeability assays human Astrocytes human brain microvascular endothelial cells Pericytes Source Type: news

More and More OR Pubs
Potent, Proven and Frequently PublishedMany labs have used our Opioid Receptor antibodies over the past 10+ years. We are grateful for the many publications and testimonals referencing successful use of these antibodies.Here's a sampling of more recent publications: Wendy M. Walwyn, Wenling Chen, Hyeyoung Kim, Ani Minasyan, Helena S. Ennes, James A. McRoberts, and Juan Carlos G. Marvizón. Sustained Suppression of Hyperalgesia during Latent Sensitization by μ-, δ-, and κ-opioid receptors and α2A Adrenergic Receptors: Role of Constitutive Activity. The Journal of Neuroscience, 6 January ...
Source: Neuromics - January 25, 2016 Category: Neuroscience Tags: DOR Antibody Kappa Opioid Receptor Antibodies KOR Antibody MOR Antibody Mu Opioid Receptor antibody Opioid Receptors Antibodies Source Type: news

Why You Should Consider Culturing Your Cells in 3-D
2-D versus 3-D and meaningful outcomesWe have seen recent growth in our customers' demand for 3-D cell based assay solutions. This could be due to more the fact that these assays our proving to generate more in vivo like data.Breast cancer cells, for example, grown in 2-D can easily be killed by low doses of chemotherapeutic drugs or low doses of radiation. If those same cells are grown in 3-D, they are resistant to the same doses of chemotherapeutic drugs or radiation, just like cancer as its found in the body. In this way, cells grown in 3D are more valid targets for testing and discovering new drugs to treat cancer. Ano...
Source: Neuromics - January 14, 2016 Category: Neuroscience Tags: 3-D Assays. 3-D imaging 3-D Cell 3-D Cell based Assays 3-D Cell Culturing 3-D collagen scaffolds 3-D nanofibers breast cancer Source Type: news

Why You Should Consider Culturing Your Cell in 3-D
2-D versus 3-D and meaningful outcomesWe have seen recent growth in our customers' demand for 3-D cell based assay solutions. This could be due to more the fact that these assays our proving to generate more in vivo like data.Breast cancer cells, for example, grown in 2-D can easily be killed by low doses of chemotherapeutic drugs or low doses of radiation. If those same cells are grown in 3-D, they are resistant to the same doses of chemotherapeutic drugs or radiation, just like cancer as its found in the body. In this way, cells grown in 3D are more valid targets for testing and discovering new drugs to treat cancer. Ano...
Source: Neuromics - January 14, 2016 Category: Neuroscience Tags: 3-D Assays. 3-D imaging 3-D Cell 3-D Cell based Assays 3-D Cell Culturing 3-D collagen scaffolds 3-D nanofibers breast cancer Source Type: news

Sleeping Beauty (SB) Transposon for the Study of Cancers
In this study, a modified SB delivery system, under control of the hTERT promoter in conjunction with the SV40 enhancer, was able to successfully transfer the suicide gene (HSV-TK) into multiple types of cancer cells. The modified SB transfected cancer cells exhibited a significantly increased cancer cell specific death rate. These data suggest that our modified SB-based gene delivery system can be used as a safe and efficient tool for cancer cell specific therapeutic gene transfer and stable long-term expression."Figure 8. The effect of modified SB system on the tumor growth in vivo.Lung cancer cells (H358) (A), pros...
Source: Neuromics - January 9, 2016 Category: Neuroscience Tags: cancer cell cultures Cancer Research Custom Transposons gene expression analysis gene integration sleeping beauty Source Type: news

CRISPR-Cas9 and Sleeping Beauty Transposons
Neuromics-B-Mogen ApproachThe CRISPR-Cas9 gene editing approach is now officially considered a revolution. This is confirmed by the increasing frequency of this word being used across the web in describing this technique. In fact, it is now appearing in conference titles. see: Genome Engineering: The CRISPR/Cas Revolution 2016COLD SPRING HARBOR - 17 AUG 2016.We have joined the revolution with our new Sleeping Beauty TransposonTM Systems.Figure: Sleeping Beauty TransposonTM Systems Gene Integration Process. (A) A depiction of Sleeping Beauty transposon plasmid and Sleeping Beauty transposase enzyme active in cellular nuclei...
Source: Neuromics - January 3, 2016 Category: Neuroscience Tags: cas9 CRISP CRISP-Cas9 gene editing gene expression analysis gene integration Sleeping Beauty. transposase transposons Source Type: news

Fibroblasts into Neurons
Via Activation of Oct4We have a variety of stem cell, progenitor and neuron markers that are often referenced in publication. There represent a qualitative way to determine the state of stem cells as they differentiate.Here're researchers take Fibroblasts and differentiate them into various progenitors. They were able to further differentiate these progenitors into astrocytes and neurons by: For neuronal differentiation, after 2 days of incubation with 20 nM reversine, cells were then treated with 0.5 μM all-trans-retinoic acid (RA) in serum-free DMEM/F-12 medium supplemented with the ITS for 2 days and switched into se...
Source: Neuromics - December 17, 2015 Category: Neuroscience Tags: fibroblasts GFAP Antibody Glial Fibrillary Acidic Protein Antibody neural progenitors Neuron Specific Enolase Antibody NSE Antibody Source Type: news

Kv Channels and Pain Transmissiom
i-Fect TMis a Proven Tool for Gene Manipulation in Studying All Types of PainI previously posted on use of our i-Fect Transfection Kit to silence Kv Channels Receptors. This has enabled researchers to study the role of these receptors in vitro and in vivo (see:i-Fect™ Delivers Your siRNA Payload).Sample DataFigure: Figures. siRNA-mediated knockdown of Kv1.1 expression in thoracic DRG significantly increased gastric sensitivity in naive adult rats. (A) Western blots showed a significant decrease in Kv1.1 protein in thoracic DRG (T8–T12) after intrathecal treatment with Kv1.1 siRNA but not with control ...
Source: Neuromics - December 9, 2015 Category: Neuroscience Tags: i-Fect KV receptors. i-Fect siRNA delivery in-vivo Source Type: news

Neuron Astrocyte Glial Markers
Potent and Frequently Published!We have a stout catalog of Neuron-Astrocyte and Glial Markers.They are widely used and frequently published. Here're some recent examples:Mouse Monoclonal GFAP: Chelsea M. Larabee, Constantin Georgescu, Jonathan D. Wren and Scott M. Plafke. Expression profiling of the ubiquitin conjugating enzyme UbcM2 in murine brain reveals modest age-dependent decreases in specific neurons. BMC Neuroscience201516:76 DOI: 10.1186/s12868-015-0194-y© Larabee et al. 2015.Image: Mixed neuron-glial cultures stained with Mouse Monoclonal GFAP, and Chicken Polyclonal Neurofilament-NF-L (green). The GFAP anti...
Source: Neuromics - November 28, 2015 Category: Neuroscience Tags: Astrocyte-Glial-Neuron Co-cultures GFAP Antibody Immunfluorescence immunoblotting Immunohistochemistry Neurofilament Markers Neuron Markers Source Type: news

Staining Neurons and 3-D
In this study researchers use our Tuj-1/Beta Tubulin Antibody to stain neurons in 3-D Cultures: Gaoying Sun, Wenwen Liu, Zhaomin Fan, Daogong Zhang, Yuechen Han, Lei Xu1, Jieyu Qi, Shasha Zhang, Bradley T. Gao, Xiaohui Bai,Jianfeng Li,Renjie Chai, Haibo Wang. The three-dimensional culture system with matrigel and neurotrophic factors preserves the structure and function of spiral ganglion neuron in vitro...Tuj-1-β-tubulin (1:1000, Neuromics, USA)... Images: Morphology of the SGNs growth cone cultured in 2D and 3D systems. Phalloidin, green; β-tubulin, red.I will continue to post positive developments concerning u...
Source: Neuromics - November 20, 2015 Category: Neuroscience Tags: 3-D Cell based Assays 3-D Cell Culturing Immunofluorescence Neurons Tuj 1 (Neuron-specific class III beta-tubulin) antibody Tuj 1 antibody Tuj-1 antibodies Source Type: news

Reconstructing 3D Living Biomaterials
In this study, researchers use our  Collagen Hydrogel, Collagen Hydrogel Soft and Collagen Hydrogel Soft+  to describe  in vivo applications using a murine model to interrogate biocompatibility and cellular behavior post-transfer.Luc macrophages (CC or BES) were then incorporated into a fl uid mixture containing one of three alternative collagen-based hydrogels, namely, Collagen Hydrogel, Collagen Hydrogel Soft, or Collagen Hydrogel Soft+ (resulting in six possible combinations of macrophages and biopolymer).Overview of ResultsFigure: Bioluminescent imaging of implanted macrophage–biopolymer mixes. IC-...
Source: Neuromics - November 11, 2015 Category: Neuroscience Tags: 3-D Assays. 3-D imaging 3-D Cell based Assays 3-D Cell Culturing 3-D collagen scaffolds Hydrogel regenerative medicine tissue engineering tissue regeneration tissue repair Source Type: news

Human alpha-Sensory Neurons
Put Them to Work!We continue to add unique primary Human Neurons to our offerings.We have discounted our new alpha-Sensory Neurons to make it easier to buy and try-only 850 USD/1,000,000 cells.Just thaw and culture. Simply culture on ECM- Laminin or Fibronectin coatings with our alpha Motor Neuron Maintaining Media. Questions? Please contact me directly 612-801-1007 or pshuster@neuromics.com. Pete Shuster, CEO and Owner, Neuromics. (Source: Neuromics)
Source: Neuromics - November 7, 2015 Category: Neuroscience Tags: Human Primary Neurons human sensory neurons. SNs neuronal cultures primary human cells Source Type: news

Solutions for Blood Brain Barrier Research
Neuromics Has the Key Components!We are pleased to add Human Brain Microvascular Pericytes (HBMVPCs) to our solution set for Blood Brain Barrier (BBB) Researchers. These join our Human Brain Microvascular Endothelial Cells and hAstroPro Human Astrocyte+Astroglial-Neurons Co-Culturing Kits to round out our offerings of cells involved in BBB regulation. Image: Human Brain Pericytes cultured with AlphaBioCoat (AC001).Pericytes function at the BBB in at least two ways: by regulating BBB-specific gene expression patterns in endothelial cells, and by inducing polarization of astrocyte end-feet surrounding CNS blood vessels ...
Source: Neuromics - October 29, 2015 Category: Neuroscience Tags: BBB Blood Brain Barrier Blood Brain Barrier Models brain microvascular endothelial cells human brain microvascular cells human brain microvascular endothelial cells Source Type: news

Brain Freeze!
Our Friend are Taking Neuromics Brain on Interesting #brainadventureIt is getting harder to select the winners. You can submit your entry now and earn a chance to win our monthly $100 monthly and $500 grand prize. To enter see: http://www.neuromics.com/brain-adventure-contest.We can all relate to this. (Source: Neuromics)
Source: Neuromics - October 21, 2015 Category: Neuroscience Tags: #BrainAdventure Source Type: news

hNP1 Neural Progenitors and Neuronal Regeneration after Injury
In this study, researchers used our hNP1TM Neural Progenitors to demonstrate that axonal PPARγ is involved in neuronal injury responses required for axonal regeneration: Juan Pablo Lezana, Shachar Y. Dagan, Ari Robinson, Ron S. Goldstein, Mike Fainzilber, Francisca C. Bronfman, and Miguel Bronfman. Axonal pparγ promotes neuronal regeneration after injury. DOI: 10.1002/dneu.22353... Culture and regeneration analysis of human axons. NP1 human neural precursors were purchased from Neuromics (Minnesota, USA) and passaged up to 8 times before generation of neurons. Cells were used up until passage 10 after defrostin...
Source: Neuromics - October 13, 2015 Category: Neuroscience Tags: hNP1 Neural Progenitors Neurite outgrowth Neuronal Differentation Neuronal Regeneration PPARgamma Source Type: news

#brainadventure Update
2 Win $100 Monthly Prices!Our customers are creative. They find interesting #brainadventures for our Neuromics' Brains. Competitive hing for winning our $100 monthly and $500 grand prizes: more entries raise your odds of winning.Here're some of the submissions:Learn how you can win @ http://www.neuromics.com/brain-adventure-contest.Good luick to all. (Source: Neuromics)
Source: Neuromics - October 1, 2015 Category: Neuroscience Tags: #BrainAdventure Source Type: news

Retinal Microvascular Endothelial Cells & Diabetic Retinopathy
In this study, the authors show that human retinal microvascular endothelial cells exposed to high levels of glucose regulate miR-200b repression through histone methylation and that inhibition of PRC2 increases miR-200b while reducing VEGF: Michael Anthony Ruiz, Biao Feng, and Subrata Chakrabarti. Polycomb Repressive Complex 2 Regulates MiR-200b in Retinal Endothelial Cells: Potential Relevance in Diabetic Retinopathy. PLoS One. 2015; 10(4): e0123987. Published online 2015 Apr 17. doi: 10.1371/journal.pone.0123987...To investigate PRC2 and miR-200b regulation in the context of diabetic retinopathy, the major cell type use...
Source: Neuromics - September 26, 2015 Category: Neuroscience Tags: Glucose HRMECS Human Endothelial Cells Human Retinal Microvascular Cells VEGF Source Type: news

Mouse and Human Motor Neurons
Designed for Neuro-muscular Diseases ResearchClients have been using our easy to culture and research proven GFP Labeled Mouse Motor Neurons for Neuro-muscular disease research. This includes the inclusion of the cells in several ALS drug discovery programs being conducted by large Pharma.Image: GFP+ mMN Mouse Motor Neurons at 2 days post thaw 20X.I am pleased to announce the addition of Human Motor Neurons to our Primary Human, Mouse and Rat Neurons, Astrocytes and Neuron-Astroglial co-culture solutions.Image: Human alpha-Motor NeuronsQuestions?  Do not hesitate to contact me directly, Pete Shuster, CEO and Owne...
Source: Neuromics - September 17, 2015 Category: Neuroscience Tags: ALS Amyotrophic Lateral Sclerosis GFP labeled mouse motor neurons Human Motor Neurons MS Multiple Sclerosis Source Type: news

hNP1 Neural Progenitors and HTS Tox Assays
This article demonstrates the sensitivity of Neuromics' hNP1TM Neural Progenitors to chemically induced apoptosis: Ingrid Druwea, Theresa M. Freudenrich, Kathleen Wallace, Timothy J. Shafer, William R. Mundy. Sensitivity of neuroprogenitor cells to chemical-induced apoptosis using a multiplexed assay suitable for high-throughput screening. doi:10.1016/j.tox.2015.03.011. Summary: The results demonstrate that, (1) all three commercially available models generated a robust source of proliferating neuroprogenitor cells, and that the assay was sensitive and reproducible when used in a multi-well plate format; (2) there were dif...
Source: Neuromics - September 11, 2015 Category: Neuroscience Tags: Apoptosis Cytotoxicity Assays Highthroughput Screening Highthroughput Toxicity Screening hNP1 Neural Progenitors Neurotoxicity Tox assays Source Type: news

Connective-Tissue Growth Factor (CTGF/CCN2) Induces Astrogenesis
SOX-2 Proves a Marker For AstrogenesisThis proved  surprising to the authors of a recent publication referencing use of our Monoclonal SOX-2 Antibody. We have added our SOX-2 abs to our Glial-Astrocyte Markers. See: Fabio A. Mendes , Juliana M. Coelho Aguiar , Suzana A. Kahn, Alice H. Reis, Luiz Gustavo Dubois, Luciana Ferreira Romão, Lais S. S. Ferreira, Hervé Chneiweiss, Vivaldo Moura Neto, José G. Abreu. Connective-Tissue Growth Factor (CTGF/CCN2) Induces Astrogenesis and Fibronectin Expression of Embryonic Neural Cells In Vitro. Published: August 4, 2015DOI: 10.1371/journal.pone.0133689...mono...
Source: Neuromics - September 3, 2015 Category: Neuroscience Tags: astrocyte cultures Astrocyte Marker Astrocyte marker antibody Atrogenesis Glial Markers sox2 antibody Source Type: news

Small Molecules/Peptides for Pain Researchers
Agonists, Antagonists, Inhibitors and LigandsThe roots of Neuromics is providing solutions for the study of nociceptive and neuropathic pain. Our tools are widely used and frequently published. We are pleased to bring you these small molecules designed to help you better understand the roots of pain and potential therapies. Figure: Infarct volume after 3 days of reperfusion in the ipsilateral cortex and CP complex in male rats treated with vehicle saline (n=15) or 1 mg/kg per hour BRL 52537 (n=15) started at onset of reperfusion and continued for 22 hours (male-vehicle n=15; male-BRL n=15; mean±SEM). P
Source: Neuromics - August 28, 2015 Category: Neuroscience Tags: agonists antagonists chronic pain Inflammatory pain inhibitors ligands Neuropathy Nociception Nociceptive Pain Peptides Small Molecules Source Type: news

Take Your Neuromics' Brain on an Adventure
Grand Prize is 500 USD!Have fun. We can't wait to see your ideas.#brainadventure (Source: Neuromics)
Source: Neuromics - August 27, 2015 Category: Neuroscience Tags: #BrainAdventure Source Type: news

Have Brain; Will Travel
Join the Adventure and You Could Win 500 USD Contest begins September 2015. Monthly winners receive 100 USD. Contest will run for 6 months. Grand prize is 500 USD.Join Neuromics on our‪#‎BrainAdventure‬ to see all the interesting and unique locations the brain ventures. Starting this September, with all orders, you will receive a Neuromics brain keychain. Take a creative picture or short video of the Neuromics Brain Keychain and tweet it @Neuromics, post it on Neuromics Facebook or LinkedIn Page and be entered in our contest. In the meantime, if you want to join the contest, e-mail rose@neuromics.com and she w...
Source: Neuromics - August 14, 2015 Category: Neuroscience Tags: #BrainAdventure Neuromics Source Type: news

Medical Marijuana Research
What we are learning.I have reported here Medical Marijuana research and its therapeutic value for treating pain. The specifics posts feature publications that reference use of our Pain and Inflammation Research Markers. These include: J. Desroches, J.-F. Bouchard, L. Gendron, P. Beaulieu. Involvement of cannabinoid receptors in peripheral and spinal morphine analgesia ☆ Neuroscience, Volume 261, 7 March 2014, Pages 23–42.http://dx.doi.org/10.1016/j.neuroscience.2013.12.030.Iryna A. Khasabova,Sergey Khasabov, Justin Paz, Catherine Harding-Rose, Donald A. Simone, and Virginia S. Seybold. Cannabinoid Type-1 Receptor ...
Source: Neuromics - August 12, 2015 Category: Neuroscience Tags: 5HT Antibodies 5HT2AR Antibody Cognition Medical Marijuana Memory Pain Source Type: news

New Human Brain Microvascular Cells
HBMECS for BBB ResearchersI am pleased by the growing positive response to our Human Endothelial Cells offerings. Our Human Brain Microvascular Endothelial Cells have, far and away, been the most popular. We want to make it easier for you to "buy and try" We are offering them for only 699 USD/500,000 Cells through Aug. 31st, 2015. Note: customers have passaged these cells up to 15 X.Image: Human Brain Microvascular Endothelial Cells in Culture.Questions? Please contact me: pshuster@neuromics.com or 612-801-1007. (Source: Neuromics)
Source: Neuromics - August 8, 2015 Category: Neuroscience Tags: BBB Blood Brain Barrier Endothelial Cell Proliferation HBMECs human brain microvascular cells Source Type: news

Gene Expression-Have it Your Way
From Delivery to Stable ExpressionNeuromics has a successful track record of helping our clients delivery siRNA, miRNA, Plasmids and other oligos in vitro and in vivo with our Transfection Kits...But my vision with our cell based assay solutions has always been to provide engineered cells and plasmids modified to study your genes of interest. I am pleased to announce we are working with Smart Cell /B-MoGen Technologies to make this happen. We now can provide:Gene Transfer and Expression Products Leveraging the Sleeping Beauty Technology: Images: B-MoGen Transposon exhibiting stable expression of five fluorescent genes.&nbs...
Source: Neuromics - August 3, 2015 Category: Neuroscience Tags: gene expression analysis Gene Silencing gene transfer lentiviruses sleeping beauty Transfection Reagents transposons Source Type: news

Neuropeptides and Stress
Neuropeptides Antagonists, Stress and Alcohol SeekingSelect Neuropeptides can help moderate stress and related Alcohol seeking behaviors. The authors of this study reference use of our ppENK and proDYN Neuropeptides: J.R. Schank,B.S. Nelson,R. Damadzic,J.D. Tapocik,M. Yao,C.E. King,K.E. Rowe,K. Cheng,K.C. Rice,M. Heili. Neurokinin-1 receptor antagonism attenuates neuronal activity triggered by stress-induced reinstatement of alcohol seeking. doi:10.1016/j.neuropharm.2015.07.009.Image: Mouse striatum stained with D2 cell marker Enkephalin (RA14124) in green and with neuronal marker NeuN in red courtesy of Dr Heike Rebholz o...
Source: Neuromics - July 21, 2015 Category: Neuroscience Tags: Alcohol Nociceptive Pain Pain Research ppENK antibody pro-Dynorhin Antibody stress Source Type: news

Apoptosis and Neurodegeneration
Towards the Development of Disease Specific AssaysNeurodegenerative diseases are becoming increasingly prevalent, especially in the Western societies, with larger percentage of members living to an older age. They have to be seen not only as a health problem, but since they are care-intensive, they also carry a significant economic burden. Apoptotic pathways are induced in many of these diseases and are key culprits in disease progression. Figure: Schematic representation of apoptotic pathways. Apoptosis triggered by internal (intrinsic) or external (extrinsic) stress signals that is activated by binding of ligands (e.g. F...
Source: Neuromics - July 13, 2015 Category: Neuroscience Tags: ALS Alzheimer ' s Disease Apoptosis Assays Astrocyte-Glial-Neuron Co-cultures FLICA in vitro Apoptosis Detection Kits Multiple Sclerosis neuron cultures neuronal cultures Parkinson Source Type: news

Save 100 USD on our hN2™ Primary Neurons
Potent, Pure and Easy to CultureAssay data continues to roll in. I am pleased by the high lot to lot consistencies of our hN2 Primary Human Neurons resulting in data reproducibility. Here're an examples of outgrowth/tox assays: In conjunction with Molecular Devices' ImageeXpress HCI platform, hN2 differentiated neuronal cells can be used to evaluate potential toxic effects of test compounds on nuerite outgrowth through visualizing cells stained with β-lll tubulin.Figure: Dose response curves for the effect of neurotoxic agents on neurite outgrowth. hN2™ cells were cultured in the presence of cytotoxic compounds ...
Source: Neuromics - June 30, 2015 Category: Neuroscience Tags: Culturing Human Neurons Protocol Cytotoxicity Assays hN2 Kits hN2 Neurons Cell Cultures Neurite outgrowth Neurotoxicity Source Type: news

Save 100 USD on Our New Cardiomyocytes
Potent, Pure and Easy to CultureI am pleased to announce the addition of Human Cardiomyocytes to our Stem and Cell Based Assay Solutions.Image: Human Cardiomyocyte culture.These cells are optimized to provide addition options for in-vitro testing of drug to drug candidate toxicities allowing researchers to rule out the ineffective and potentially toxic small molecules/compounds early in the process.I want to make it easy for you to buy and try. If you are not delighted with your results, I will replace free of charge or refund your purchase. Please also note the select positive feedback on our other primary and stem cells:...
Source: Neuromics - June 24, 2015 Category: Neuroscience Tags: cardiomyocyte based assays cardiomyocytes Cell based assays Cytotoxicity Assays Drug Discovery Human Mesenchymal Stem Cells Source Type: news

Voices of our Customers
Your Feedback Matters-A lotOur follow up processes include actively engaging users of our solutions for feedback and input. This includes Rose Ludescher, Manager of Customer Satisfaction, calling or sending e-mails to each user within 3 weeks of shipment. At the core is our product rating website. If users identify products related issues, we replace them or refund their purchases.We want to hear from you...the good, the bad and the ugly. We welcome opportunities to fix issues.We are always pleased when customers share data with us. Here's a recent example.Image: Mouse striatum stained with D2 cell marker Enkephalin (RA141...
Source: Neuromics - June 22, 2015 Category: Neuroscience Tags: Customer Service Neuromics Neuropathy Nociception Pain Research Source Type: news

Network vs Isolated Bursting in Motor Neurons
Motor Neurons and MEA Dysregulated bursting is at the root of many motor neuron/neuromuscular junction disease. ArunA Biomedical teaming with Axion Biosystems have generated relevant bursting data from our Mouse Motor Neurons cultured on Axion-Bioystem's Maestro MEA.Figure: Mouse Motor Neuron Network Modulation by Bicuculline-ckeck out the entire presentation to learn more: GFP+ Motor Neurons: Development and in-vitro Functional Assessment on Microelectrode Arrays Protocol User's Guide for Culturing Motor Neuron on MEA(pdf - 679Kb)ArunA Biomedical's/Neuromic's Mouse Motor Neurons on Axion Biosystem's MEASave on Mouse Motor...
Source: Neuromics - June 15, 2015 Category: Neuroscience Tags: Bursting Assay GFP labeled mouse motor neurons MEA Microelectrode Arrays Neural Networks Neuronal Networks Synchronized Bursting Source Type: news

MEA and Motor Neurons
Plating Densities of Motor Neurons Matter!I wanted to share some of the tips and data shared during the ArunA Biomedical's/Axion Biosystem's Webinar on MEA and our Mouse Motor NeuronsPlating Cells on Axion's MEASurface Coating PEI-laminin for adhesion and uniform monolayer development Dotting Constrains cells to the array Requires fewer cells per well Media changes every 2-3 daysVariations on Cell DensityDetailed Protocol for Culturing Motor Neurons on MEA Different Plating DensitiesPlating Densities between 60,000-80,000 OptimalAt these densities there was the least variation in mean firing rates. This data...
Source: Neuromics - June 10, 2015 Category: Neuroscience Tags: ALS Axion Bioystems Firing Rates GFP labeled mouse motor neurons MEA Microelectrode Arrays motor neuron diseases Plating Densities Source Type: news

Neuromics' Neurononal Cultures in Action
Drug Discovery and Tox Assay PublicationsOur Primary Human, Rat and Mouse Neurons are widely used and frequently cited in publications. Here's a pub hot of the presses referencing use of our e18 Rat Cortical Neurons to study the EPO-like cytoprotective effects cultures of these cells: James L. Miller, Timothy J. Church, Dmitri Leonoudakis, Karen Lariosa-Willingham, Normand L. Frigon, Connie S, Tettenborn, Jeffrey R. Spencer, and Juha Punnonen. Discovery and Characterization of Nonpeptidyl Agonists of The Tissue-Protective Erythropoietin Receptor. Molecular Pharmacology. May 27, 2015 mol.115.098400...Cells were is...
Source: Neuromics - June 3, 2015 Category: Neuroscience Tags: e-18 Primary Mouse Neurons e-18 Primary Rat Cortical Neurons EPO hN2 Neuron Primary Human Neurons primary Neuronal Cells s Cell Cultures Source Type: news

Neuromics' Stem Cell Markers
Potent, Proven and PublishedWe have a growing parade of Publications referencing use of our Stem Cell Markers. They have proven to be excellent for assays using our hNP1™ Human Neural Progenitor Kits and Human Mesenchymal Stem Cells (hMSCs),Here's a recent reference highlighting use of our Nucleostemin Antibody: Zhe Ding and He Huang. Mesenchymal stem cells in rabbit meniscus and bone marrow exhibit a similar feature but a heterogeneous multi-differentiation potential: superiority of meniscus as a cell source for meniscus repair. BMC Musculoskeletal Disorders (2015) 16:65 DOI 10.1186/s12891-015-0511-8. ...After remov...
Source: Neuromics - May 29, 2015 Category: Neuroscience Tags: BMSCs Bone marrow derived stem cells meniscus-derived mesenchymal stem cells MMSCs Nucleostemin Nucleostemin Antibody Stem Cell Antibodies Stem cell markers Source Type: news

GFP Labeled Motor Neurons and MEA
Big Upcoming WebinarWe have been strategically partnering with ArunA Biomedical to improve how we serve Neuro-drug discovery and Neurotox research community. I am especially excited about how well are our GFP Labeled Mouse Neurons are working in many different and unique research applications. They have proved an important tool in the study of neuro-muscular diseases like ALS, Parkinson's and Multiple Sclerosis.I am pleased to announce a coming Webinar: “GFP+ Motor Neurons: Development and in-vitro Functional Assessment” Wednesday June 10th, 11:30 AM EDT-Register Today!Download Flyer. I will continue to post un...
Source: Neuromics - May 22, 2015 Category: Neuroscience Tags: ALS Astrocyte-Glial-Neuron Co-cultures GFP labeled mouse motor neurons human Astrocytes MEA motor neuron diseases Multiple Sclerosis Parkinson ' s Disease Source Type: news