MEA and Motor Neurons

Plating Densities of Motor Neurons Matter!I wanted to share some of the tips and data shared during the ArunA Biomedical's/Axion Biosystem's Webinar on MEA and our Mouse Motor NeuronsPlating Cells on Axion's MEASurface Coating PEI-laminin for adhesion and uniform monolayer development Dotting Constrains cells to the array Requires fewer cells per well Media changes every 2-3 daysVariations on Cell DensityDetailed Protocol for Culturing Motor Neurons on MEA Different Plating DensitiesPlating Densities between 60,000-80,000 OptimalAt these densities there was the least variation in mean firing rates. This data shows as the density increased the cells moved towards firing in synchronicity.Figure: Difference in Mean Motor Neurons Firing Rates vs Plating DensitiesThe demand for using these motor neurons for neuromuscular diseases drug discovery has been brisk and growing. Should you have question on how they would work for your unique applications, do not hesitate to contact me directly @ 612-801-1007 or pshuster@neuromics.com. Pete Shuster, CEO and Owner, Neuromics
Source: Neuromics - Category: Neuroscience Tags: ALS Axion Bioystems Firing Rates GFP labeled mouse motor neurons MEA Microelectrode Arrays motor neuron diseases Plating Densities Source Type: news