Sex Differences on the Behavior of Human Brain Microvascular Endothelial Cells (HBMECS)
The Risk of StrokeThere are sex differences in risk for stroke and small vessel ischemic disease in the brain. Microvesicles (MV) derived from activated cells vary by cell of origin and the stimulus initiating their release. MV released from cells activated by inflammatory and thrombotic factors have the potential to disrupt endothelial cells of the brain microvasculature. Therefore, experiments were designed to identify sex differences in the phenotype of MV released from cultured human brain microvascular endothelial cells (HBMEC) in response to inflammatory and thrombotic stimuli (Biology of Sex Differences201910:26http...
Source: Neuromics - June 19, 2019 Category: Neuroscience Tags: Blood Brain Barrier brain microvascular endothelial cells HBMECs Microvasculature Stroke Source Type: news

Human Pancreatic CAFS and Tumor Dynamics
 Extracellular Matrix (ECM) MattersOurHuman Pancreatic Cancer Fibroblasts were recently featured in a publication focusing on the role of the ECM on tumor dynamics. Andreas Stylianou, Vasiliki Gkretsi, Maria Louca, Lefteris C. Zacharia, and Triantafyllos Stylianopoulos (2019).Collagen content and extracellular matrix cause cytoskeletal remodelling in pancreatic fibroblasts. J. R. Soc. Interface 16: 20190226. http://dx.doi.org/10.1098/rsif.2019.0226.Commercially available pancreatic native human FBs and,CAFs (cat. nos. SC00A5 and CAF08, respectively, Neuromics) were cultured in MSC-GRO (VitroPlus III, low serum, comple...
Source: Neuromics - May 29, 2019 Category: Neuroscience Tags: 3D collagen scaffolds Human Pancreatic Fibroblasts Pancreatic CAF-Stellate Cells pancreatic cancer Source Type: news

Neurofilament Antibodies-Check Them Out
Widely Used and Frequently PublishedOurNeurofilament Antibodies are well characterized and research ready. Here is a recent publication using one of ourNeurofilament Heavy Antibodies. Jennifer M. Hahn, Kelly A. Combs, Christopher M. Lloyd, Kevin L. McFarland, Steven T. Boyce, and Dorothy M. Supp.Identification of Merkel cells associated with neurons in engineered skin substitutes after grafting to full thickness wounds. PLoS One. 2019; 14(3): e0213325. Published online 2019 Mar 5. doi: 10.1371/journal.pone.0213325.Figures: Merkel cells in grafted ESS are associated with neurons expression neurofilament heavy (NF-M) by eigh...
Source: Neuromics - May 22, 2019 Category: Neuroscience Tags: Neurofilament Antibody Neurofilament Markers Neurofilament-H Antibody Neuronal Markers NF-H antibody Source Type: news

We Can Sell and Deliver FBS in Large Volumes
This is Includes Single LotsWe are pleased with the growth in our FBS business. This is being made possible, in part, to our ability to find the " sweet spot " by coupling low pricing with high quality.We offer all potential customers free samples to test with the idea that they find the " sweet spot. " This leads to happy customers and repeat business.Here's a volume shipment wrapped and ready to go.2 pallets of 700X500 ml. Bottles FBS.Need FBS? Just ask us. Learn more by emailing rose@neuromics.com. Thank you! (Source: Neuromics)
Source: Neuromics - May 13, 2019 Category: Neuroscience Tags: FBS Fetal Bovine Serum Source Type: news

i-Fect Scores Again
This study is the first to demonstrate that the stabilization of HIF1A expression underpins the development of bortezomib-induced neuropathic pain. Crucially, these findings reveal that the initiation and maintenance of bortezomib-induced neuropathic pain are regulated by distinct mechanisms. (Source: Neuromics)
Source: Neuromics - May 13, 2019 Category: Neuroscience Tags: chemotherapy i-Fect RNAi siRNA siRNA delivery in-vivo Source Type: news

We Have Human Bone Marrow Derived Stem Cells
Pure and Potent-Only $655/500,000 CellsHuman Mesenchymal Stem Cells isolated from bone marrow and provided a low passage. These cells have the ability to be passaged five to 10 passages in our Low Serum, Complete Medium (#SC00B1); which is optimized for high growth rates, reduced doubling times, healthy cells and stability. This Product is manufactured, tested and validated in an ISO 9001/ISO13485/CLIA certified facility. (Source: Neuromics)
Source: Neuromics - April 27, 2019 Category: Neuroscience Tags: human Mesenchymal Stem Cell Based Assays Human Mesenchymal Stem Cells Source Type: news

Gut Brain Axis
Helicobacter pylori Induced Anxiety and AnorexiaOurPGP9.5 was used as a Hypothalamic Neuronal Marker in this study-Hajime Suzuki, Koji Ataka, Akihiro Asakawa, Kai-Chun Cheng, Miharu Ushikai, Haruki Iwai, Takakazu Yagi, Takeshi Arai, Kinnosuke Yahiro, Katsuhiro Yamamoto, Yoshito Yokoyama, Masayasu Kojima, Toshihiko Yada, Toshiya Hirayama, Norifumi Nakamura& Akio Inui (2019).Helicobacter pylori Vacuolating Cytotoxin A Causes Anorexia and Anxiety via Hypothalamic Urocortin 1 in Mice. Scientific Reports volume 9, Article number: 6011.Images: Ucn1- and DAPI-positive cells (yellow arrow heads), or Ucn1- and PGP9.5-positive c...
Source: Neuromics - April 17, 2019 Category: Neuroscience Tags: Gut-Brain Axis. Hypothalamus Helicobacter pylori Hypothalamic Neurons PGP9.5 Antibody Protein Gene Product 9.5 Source Type: news

Your Data Wanted
Reward is $50 Amazon Gift CardWe are always honored when customers share theirdata and images. More is better! This month we would like to reward you for any data or images featuring any of our products you share with us.Just e-mail Rose Ludescher, Manager of Customer Satisfaction, atrose@neuromics.com, and she will e-mail you a $50 Amazon Gift Card.Example DataHUMAN PANCREATIC CAF-STELLATE CELLS-Stained with alpha-SMA + DAP1 20x. Courtesy of Emily Rodela, TGEN-Courtesy of Emila Rodela, T-Gen. (Source: Neuromics)
Source: Neuromics - April 3, 2019 Category: Neuroscience Source Type: news

Human and Animal Cardiomyocytes
Pure, Potent and Easy to CultureCheck out our hMSC Derived Human Cardiomyocytes-These cells are optimized to provide addition options for in-vitro testing of drug to drug candidate toxicities allowing researchers to rule out the ineffective and potentially small compounds/toxic compounds early in the process.Human Cardiomyocytes in CultureNeuromics also offersprimary rat and mouse cardiomyocyte tissue and disassociated cells. (Source: Neuromics)
Source: Neuromics - March 19, 2019 Category: Neuroscience Tags: cardiomyocytes human cardiomyocytes Mouse Cardiomyocytes Rat Cardiomyocytes Source Type: news

Neuron-Glial Markers
Featuring Neurofilament AntibodiesWe have the honor of ourNeuron-Glial Markers being referenced in manypublications. Here we wanted to feature some recent data from one of ourNeurofilament Antibodies.Fig. Merkel cells in grafted ESS are associated with neurons expression neurofilament heavy (NF-M) by eight weeks after grafting. Immunochemistry with antibodies against NF-H (red) and KRT20 (green) was used to localize neurons and Merkel cells, respectively, in ESS after grafting to mice. Nuclei were counterstained with DAPI (blue; B, D, F, H). Shown are cross sections of ESS at 4 weeks (A-B), 8 weeks (C-D), 12 weeks (E-F), a...
Source: Neuromics - March 8, 2019 Category: Neuroscience Tags: Glia Markers Neurofilament Antibody Neurofilament Markers Neuronal Markers NF-H antibody Source Type: news

Neuromics' Human Brain Pericytes Guide Axon Growth
Study Interactions Between Blood Vessels and Nerve CellsOurGFP-Labeled Human Brian Pericytes were used by Spinal Cord Injury Researchers to evaluate the efficacy of aligned microvessels to induce and control directional axon growth from neural progenitor cells in vitro and host axons in a rat spinal cord injury model. Interstitial fluid flow aligned microvessels generated from co-cultures of cerebral-derived endothelial cells and pericytes in a three-dimensional scaffold. Paul P. Partyka, Ying Jin, Julien Bouyer, Angelica DaSilva, George A. Godsey, Robert G. Nagele, Itzhak Fischer& Peter A. Galie (2019). Harnessin...
Source: Neuromics - February 27, 2019 Category: Neuroscience Tags: Axon growth axonal guidance Human Brain Pericytes Neurons Spinal cord injury Spinal cord injury repair spinal cord regeneration Source Type: news

HUVECS Guide Axon Growth
Study Interactions Between Blood Vessels and Nerve CellsOurGFP-Labeled Human Brian Pericytes were used, by researchers, to evaluate the efficacy of aligned microvessels to induce and control directional axon growth from neural progenitor cells in vitro and host axons in a rat spinal cord injury model. Interstitial fluid flow aligned microvessels generated from co-cultures of cerebral-derived endothelial cells and pericytes in a three-dimensional scaffold. Paul P. Partyka, Ying Jin, Julien Bouyer, Angelica DaSilva, George A. Godsey, Robert G. Nagele, Itzhak Fischer& Peter A. Galie (2019). Harnessing neurovascular i...
Source: Neuromics - February 27, 2019 Category: Neuroscience Tags: Axon growth axonal guidance Human Brain Pericytes Neurons Spinal cord injury Spinal cord injury repair spinal cord regeneration Source Type: news

Sorting Cancer-Associated Fibroblasts (CAFs)
Featuring Our Colorectal CAFSCAFs play a central role in the Tumor Microenvironment (TME). The TME has been identified as one of the driving factors of tumor progression and invasion. Inside this microenvironment, CAFs, a type of perpetually activated fibroblasts, have been implicated to have a strong tumor modulating effect and play a key role in areas such as drug resistance.This makes CAFs a target for cancer therapies. The challenge is the TME is heterogeneous making it a challenge to derive homogeneously relevant populations for basic research and drug discovery. This new study uses ourColorectal CAFs to identify...
Source: Neuromics - February 14, 2019 Category: Neuroscience Tags: CAFs Cancer Associated Fibroblasts CD90 colorectal CAFs Colorectal Cancer Flow cytometry PDGF Source Type: news

Neuromics Human Cells at Work
Work Great in 3-D Assays Neuromics Human Primary Cells are being used with increasing frequency in3-D Cell-Based Assays. Last month, we posted a publication referencing use of ourHuman Pericytes in a Blood-Brain Barrier Model.In this publication, our GFP-Labeled HUVECS are used in a 3-D Microfluidics Chip Model to study the impact of indoor airborne particles on human health. Yan Li, Chuanlin Hu, Pengcheng Wang, Yan Liu, Luyang Wang, Qingmeng Pi, Zhiyong Gong, Xu Yang, Michael Mak and Yang Wu (2019).Indoor nanoscale particulate matter-induced coagulation abnormality based on a human 3D microvascular model on a microfl...
Source: Neuromics - February 7, 2019 Category: Neuroscience Tags: Brain Perivascular Cells Human Brain Pericytes human umbilical cord endothelial cells huvecs Microfluidics Source Type: news

Neuromics' Islet-1 Used to Study Avian Heart Morphology
Evolution of High-Performance Hearts OurIslet-1 Antibody showed its versatility a comparative morphology of avian hearts.Figure: Sinuatrial node in Mallard (a –c), chicken HH42 (d–g), Lesser redpoll (h–j), and Jackdaw (k–m). All sections are in the transverse plane. The boxed areas in the left-hand column images indicate the areas of the images of the middle and right-hand columns. (a–c) In the Mallard, a nodal structure at the base of the right leaflet of the sinuatrial valve (black arrowhead in [a]) expressed Isl1 (b, c) and had a large coronary artery (white arrowhead in [b]). Sections 271 ...
Source: Neuromics - January 28, 2019 Category: Neuroscience Tags: Comparative Morphology Avian Hearts Isl-1 islet-1 antibody Source Type: news

Markers for the Study of Diabetic Neuropathy
Widely Used and Frequently PublishedOurPain and Inflammation Research Antibodies have proven valuable for the study of neuropathic, nociceptive and inflammatory pain.This recent publication is focused on Diabetic Neuropathy. Jos é Eduardo Roa-Coria, Jorge Baruch Pineda-Farias, Paulino Barragán-Iglesias, Geovanna Nallely Quiñonez-Bastidas, Ángel Zúñiga-Romero, Juan Carlos Huerta-Cruz, Juan Gerardo Reyes-García, Francisco Javier Flores-Murrieta, Vinicio Granados-SotoView ORCID ID profile and Héctor Isaac Rocha-Gonzál ez (2019).Possible involvement of periphe...
Source: Neuromics - January 18, 2019 Category: Neuroscience Tags: Diabetic Neuropathy Hydrogen Sulfide Substance P TRP antibody Source Type: news

Rat Hippocampal Neurons
Used to Study Apoptosis Induced by Brain InsultsAlthough ourprimary human cells are increasingly being used in drug discovery and toxicology assays versusanimal cells, we are always pleased to see the success of the latter in sophisticated studies.This publication references use of oure18 rat hippocampal neurons. These cells continue to beeasy to culture, pure and potent. Chiara Porro, Antonia Cianciulli, Teresa Trotta, Dario Domenico Lofrumento, Rosa Calvello and Maria Antonietta Panaro. (2019).Formyl-methionyl-leucyl-phenylalanine Induces Apoptosis in Murine Neurons: Evidence for NO-Dependent Caspase-9 Activation. Biolog...
Source: Neuromics - January 9, 2019 Category: Neuroscience Tags: cell culturing protocol E18 Primary Hippocampal Neurons fMLP Formyl-methionyl-leucyl phenylalanine primary Neuronal Cells Source Type: news

Human Primary Cells Trifecta
Tri-culture of Neuromics'Human Primary Cells in 3-D BBB Model I mentioned in an earlier post that we take Researchers questioning the validity of ourHuman Primary Cells very seriously. We follow up with our clients to make sure our cells are working as expected. If they are not 100% happy, we offer a free replacement or full refund....So it is encouraging to see Researchers using ourHuman Pericytes, Brain Microvascular Endothelial Cells (HBMES) andAstrocytes to build a static 3-D Blood-Brain Barrier Model.Ece Bayir, M. Mert Celtikoglu, Aylin Sendemira.The use of bacterial cellulose as a basement membrane improves...
Source: Neuromics - January 1, 2019 Category: Neuroscience Tags: Astrocytes BBB Model Blood Brain Barrier Models –brain barrier penetration brain microvascular endothelial cells Brain Perivascular Cells HBMECs Human Brain Pericytes Source Type: news

Primary Human Brain Pericytes
Pure and PotentThe authors of a chapter on pericytes published in SpringerLink referred to ourhuman primary pericytes as " pericyte-like " , but chose not to characterize them because of the " exorbitant cost " . Dore-Duffy P., Esen N. (2018) The Microvascular Pericyte: Approaches to Isolation, Characterization, and Cultivation. In: Birbrair A. (eds) Pericyte Biology - Novel Concepts. Advances in Experimental Medicine and Biology, vol 1109. Springer, ChamGiven that these pericytes are also part of our hot selling3-D Human BBB Model, we are in the process of challenging these claims. We will always ...
Source: Neuromics - December 27, 2018 Category: Neuroscience Tags: 3-D Blood-Brain Barrier Model Actin Antibody BBB Model Blood Brain Barrier Models Brain Perivascular Cells Desmin Antibody Human Brain Pericytes Immunofluorescence Source Type: news

Neuromics FBS at 259 USD/500 ml.
Only 2 Weeks LeftWe wanted to get ourFBS in the hands of more cell culturing experts. Our low pricing has accelerated purchasing of this culture supplement.More importantly, we are pleased with the positive feedback. Here are some recentreviews.Linda S.on BirdEye  ★★★★★9 days agoMy experience with Neuromics was very positive. I asked for a sample of FBS and after to use the FBS I received. Peter T.on BirdEye ★★★★★month agoI purchased FBS from Neuromics. It was shipped to Canada without any problems. It arrived safely and still frozen. Cells grow well in the product. Customer ser...
Source: Neuromics - December 15, 2018 Category: Neuroscience Tags: FBS Fetal Bovine Serum Source Type: news

Pain Receptors and Diabetic Neuropathy
We Got the MarkersOurMarkers for Pain Research are widely used and frequently published. They are often referenced in publications on Diabetic nerve pain.Here are some of my past blog postings:Cytokines and Neuropathic PainEpidermal Nerve Fibers in Neuropathic Pain ModelPain Research and Gene Expression AnalysisHere's a model of the actual nerve receptors behind both intractable pain and loss of sensation.A: Simplified model of nociception under normal conditions. Free nerve endings transduce a painful stimulus into a neural signal, which propagates to DRG centrally and eventually synapses on a nociceptive neuron within th...
Source: Neuromics - November 27, 2018 Category: Neuroscience Tags: Diabetes Research Diabetic Neuropathy Diabetic retinopathy GABA NMDAR P2X3 TRPV1 Source Type: news

Primary Human Schwann Cells
Research ReadyOurSchwann Cells have proven " rock solid " in the hands of our users.Image: Human Primary Schwann Cells in CultureHuman Schwann Cells (HSwC) are isolated from the human spinal nerve. HSwC are cryopreserved at passage one and delivered frozen. Each vial contains>5 x 10^5 cells in 1 ml volume. HSwC are characterized by immunofluorescence with antibodies specific to S100, GFAP, and CD90 (Source: Neuromics)
Source: Neuromics - November 17, 2018 Category: Neuroscience Tags: human primary schwann cells Schwann Cell Markers Source Type: news

Cardiomyocytes
Pure, Potent and Research ReadyWe are recognized for our growing catalog of human primary and stem cells.These include hMSC DerivedCardiomyocytes.Human Cardiomyocytes stained with Cardiac Tryponin T (green) and counter stained with DAPI (blue).These cells are optimized to provide additional options for in-vitro testing of drug to drug candidate toxicities allowing researchers to rule out the ineffective and potentially toxic small molecules/compounds early in the process. (Source: Neuromics)
Source: Neuromics - October 27, 2018 Category: Neuroscience Tags: cardiomyocyte based assays cardiomyocytes human cardiomyocytes Source Type: news

TRP Antibodies
Some of the Best AvailableOur TRP Channel Antibodies are widely used and frequentlypublished.Here's some data from researchers using on of ourTRPV1 Antibodies.Immunohistochemical expression of serum response factor in rat dorsal root ganglia. Dorsal root ganglia dissected from male Sprague-Dawley rat and imaged by confocal microscopy for (A) TRPV1 (blue), (B) AKAP (AKAP150, green), and (C) SRF (red), with colocalization represented in panel (D). White arrows indicate cells expressing all 3 proteins. Yellow line represents 50 μM in length; images are representative of 4 individual sections taken from 6 L4 to L6 DRG. (E) ...
Source: Neuromics - October 18, 2018 Category: Neuroscience Tags: Transient Receptor Potential Vanilloid 1 Receptor Antibodies TRP antibody TRPV1 antibodies TRPV1 antibody VR1s Source Type: news

Neuromics New Neuronal Markers
Best of the BestWe are recognized for having some of the bestNeuronal-Glial Antibodies in the business. We are pleased to announce the addition of these:CH22122– Tyrosine HydroxylaseGT22101 - GFPGT22102– MAP2GT22103 - VimentinMO22186– Tyrosine HydroxylaseRA22135– Tyrosine HydroxylaseImmunofluorescent analysis of rat brainstem section stained withGT22102, MAP2dilution 1:2,000 in red, and costained with mouse mAb to MO22121, MBP dilution 1:5,000, in green. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was postfixed for 24 hours, cut to 45 μM, and free-floating sections wer...
Source: Neuromics - October 7, 2018 Category: Neuroscience Tags: Immunofluorescence MAP2 antibody MBP Antibody Myelin Basic Protein TH antibody Tyrosine Hydroxlase antibody Western Blotting Source Type: news

Quality FBS-Only 259 USD/500 ml.
First Come; First ServeWe have an inventory of FBS of approximately 2000 bottles.  This inventory includes the lots we've been selling and using internally all year.  These have proven to be of the highest quality.Here ’s a sampling of user feedback: After a referral from an ex-colleague, we tested a sample from a single batch and found that the product is great for cell cloning& hybridoma work, and generally for all other cell culture. We subsequently ordered several bottles of FBS. The sales person was very friendly and worked with me to make the purchase possible. The order arrived pro...
Source: Neuromics - September 30, 2018 Category: Neuroscience Tags: 3-D Cell based Assays Cell Cultures FBS Fetal Bovine Serum Source Type: news

Human Brain Microvascular Endothelial Cell in Action
Recent PubsOur HumanBrain Microvascular Endothelial Cells (HBMECs) are known for the performance in drug discovery and tox assays. These 21-CFR compliant cells are used in the " blood side " of our3-D BBB Models.Shavali Shaik, Bridget Kennis, Shinji Maegawa, Keri Schadler, Yang Yanwen, Keri Callegari, Rishi R. Lulla, Stewart Goldman, Javad Nazarian, Veena Rajaram, Jason Fangusaro, and Vidya Gopalakrishnan. (2018).REST upregulates gremlin to modulate diffuse intrinsic pontine glioma vasculature. Oncotarget. 2018 Jan 12; 9(4): 5233 –5250. doi: 10.18632/oncotarget.23750 Hu et al. (2016). Epigenetic Activa...
Source: Neuromics - September 20, 2018 Category: Neuroscience Tags: Blood Brain Barrier Blood Brain Barrier Models brain microvascular endothelial cells HBMECs human brain microvascular Source Type: news

Neuromics' BBB Model and Permeability Assays
Crossing the Blood-Brain BarrierOur BBB Model is being increasingly used by Bio-Pharma for drug permeability studies. Customers include Amgen, Genentech, Boehringer Ingelheim, and Merck.These assays are key for making sure molecules/compounds of interest will cross the barrier into the brain and at what rate. This data, in part, add clarity on best candidates to move intoin-vivotesting. Human Blood Brain Barrier Model 3D4500212 well Human Blood Brain Barrier Model 3D4500224 well Human Blood Brain Barrier Model 3D450026 wellsI you have interest in using our models, Rose Ludescher, Manager of Customer Satisfaction, can ...
Source: Neuromics - September 14, 2018 Category: Neuroscience Tags: Astrocytes BBB Model Blood Brain Barrier Blood Brain Barrier Models brain microvascular endothelial cells Pericytes Source Type: news

Energize your Cell-Based Assays
Check out our AlphaBioCoat Collagen is a fibrous protein found in the extracellular matrix and connective tissue. The most common form of collagen is type I and is most prevalent in bone, tendon and skin. It consists of 3 intertwined coiled subunits: 2 x α1 (I) chains and 1 x α2 (I) chain. Each chain contains 1050 amino acids wound tightly around one another in a characteristic right-handed triple helix. The triple-helical structure of collagen arises from unique abundance of the amino acids in collagen appear in a characteristic repeating motif G ly-X-Y, where X is usually proline and Y is usually hydroxy...
Source: Neuromics - August 27, 2018 Category: Neuroscience Tags: AlphBioCoat Cell Cultures cell culturing protocol Source Type: news

Human Brain Cells
Our ExpertiseWhen researchers are considering using ourhuman brain cells, they often voice concerns about growth. In order to address these concerns, we explain that we have optimized our media and coatings in order to meet their assay needs.Here's an example of how ourhuman brain pericytes grow over time.We have primary human neurons, astrocytes, microglia and Schwann cells.If you have interest you can contact us at 866-350-1500 or rose@neuromics.com. We will provide the information required to help you make an informed decision. (Source: Neuromics)
Source: Neuromics - August 16, 2018 Category: Neuroscience Tags: astrocyte cultures Culturing Human Neurons Protocol Human Microglia human primary astrocytes human primary microglia Human Primary Neurons human primary schwann cells Source Type: news

Human Brian Cells
Our ExpertiseWhen researchers are considering using ourhuman brain cells, they often voice concerns about growth. In order to address these concerns, we explain that we have optimized our media and coatings in order to meet their assay needs.Here's an example of how ourhuman brain pericytes grow over time.We have primary human neurons, astrocytes, microglia and Schwann cells.If you have interest you can contact us at 866-350-1500 or rose@neuromics.com. We will provide the information required to help you make an informed decision. (Source: Neuromics)
Source: Neuromics - August 16, 2018 Category: Neuroscience Tags: astrocyte cultures Culturing Human Neurons Protocol Human Microglia human primary astrocytes human primary microglia Human Primary Neurons human primary schwann cells Source Type: news

New Neuronal Markers
Check Them Out!We continue to addnew antibodies. Many are additions to our frequently used and widely publishedNeuron-Glial Markers.Image: Immunofluorescent analysis of cortical neuron-glial cell culture from E20 rat stained with mouse mAb to GAP43, MO22170, dilution 1:1,000, in red, and costained with chicken pAb to MAP2, dilution 1:10,000, in green. The blue is DAPI staining of nuclear DNA. GAP43 antibody labels protein expressed in the axonal membrane of the neuronal cells, while the MAP2 antibody stains dendrites and perikarya of neurons. Name Species Applications Aurora A/B Kinase Human, Mouse, Rat ICC, WB, IHC, IF Au...
Source: Neuromics - August 7, 2018 Category: Neuroscience Tags: DJ1 Antibody GAP43 Antibody Ki67 antibody. Vimentin Antibody nestin antibody OPA1 Antibody Tuj 1 antibody Source Type: news

Easy Immunostaining Staining
10X the Signal at a Fraction of the CostTime is money. This is especially true of routine immunostaining assays.We are pleased to introduce you toEZ-HRPTM Polymer Detection. Features include:10-15 times more sensitive than conventional avidin-biotin detection Allows for entire staining procedure to be completed in just under 2 hours Requires less primary antibody (3-4 times less) Does not produce non-specific background staining Can be used on unfixed or chemical fixed (formalin, formaldehyde, alcohol, etc), paraffin-embedded and frozen tissue sections.Comparison of IHC images using EZ-HRP ™ Polymer vs. a c...
Source: Neuromics - July 31, 2018 Category: Neuroscience Tags: HRP-Staining. Immunofluorescence Immunonostaining Source Type: news

Our Hearing Adapts in Space
This study uses ourShank 1a Antibody to determine changes in the neuronal structure of the ear in microgravity.Image: Verification of antibodies to CtBP2 and Shank1a. A and B: serial 14- µm cryosections were obtained from a postnatal day 71 (P71) mouse utricle, and maximum-intensity projections are shown. Hair cell (hc) and support cell (sc) nuclei are illuminated by the DAPI stain (blue). Numerous closely associated CtBP2-and Shank1a-positive puncta can be observed in the positive immunostained section represented in A (block arrowheads). The CtBP2-positive puncta highlighted by the flared arrowhead in A m...
Source: Neuromics - July 24, 2018 Category: Neuroscience Tags: hair cells microgravity Shank 1a antibody Source Type: news

Staining Cells and Tissue
The background is Bad!Yes, it is. It compromises data. We have a solution.FluoMuteTMFluoMute ™ ready-to-use reagent to reduce autofluorescence in cells and tissue. Just incubate fixed cells of tissue sections with FluoMute™ for 30-60 min at room temperature, rinse with PBS and continue with immunofluorescence ICC or/and IHC protocols. Treatment with FluoMute™ does not affect cell morp hology and the integrity of tissue antigens to be detected with primary antibodies (see bottom image). FluoMute™ is compatible with paraffin-embedded and frozen tissue sections, stem cells, lymphocytes and mammalian ce...
Source: Neuromics - July 19, 2018 Category: Neuroscience Tags: autofluorescence FluoMute Source Type: news

Human Cells in Action
Human Microvascular Retinal Endothelial Cells (HMRECS)We have built the foundation ofNeuromics on satisfied customers. We make a practice of following up with each user to make sure our solutions are working as expected. If not, we offer " no question asked " refunds or replacements.This is especially important for ourhuman cells,media, and supplements. Success with these is easy to measure as either the cells are healthy and happy or they are not.We also usereviews andpublications as another measure of satisfaction. Here I would l highlight the latest publication using ourHREMCS. A. P. Da Cunha, Q. Zha...
Source: Neuromics - July 11, 2018 Category: Neuroscience Tags: Culturing Media endothelial cells FBS Fetal Bovine Serum HRMECS Human Endothelial Cells Source Type: news

Neuromics' Fetal Bovine Serum (FBS) Strikes Again
Potent FBS at Pricing You'll LikeNeuromics started providingFBS to researchers in early 2017. Our goal was to provide thorough tested and 9-CFR compliant FBS with the lowest pricing anywhere.In order to ensure our initial claims are trustworthy, we follow up with all our users and ask that they providefeedback. Here's the latest review-Ordered Fetal bovine serum. Best price and quality" - Juwen D, Albert Einstein College of Medicine. Product: Heat-inactivated FBS, cat no. FBS001-HI.We are now starting to see the use of our FBS reference in publications: Am élie Robert, Peirun Tian, Stephen A. Ada...
Source: Neuromics - July 2, 2018 Category: Neuroscience Tags: 3-D Cell based Assays cell biology Cell Cultures FBS Fetal Bovine Serum Source Type: news

You'll Love our FBS!
Check out Testimonials from UsersNeuromics is proud to offer you the high-quality Fetal Bovine Serum, the same serum we use internally, without the high cost. Our customers, researchers like you, agree.Ordered Fetal bovine serum. Best price and quality" - Juwen D, Albert Einstein College of Medicine. Product: Heat-inactivated FBS, cat no. FBS001-HIOutstanding service. Fetal bovine serum was of highest quality, easy to order and packaging and shipping were best possible. " - Rosemary, University of Buffalo Product: Heat-inactivated FBS, cat no. FBS001-HI (see all of Neuromics reviews&...
Source: Neuromics - June 28, 2018 Category: Neuroscience Tags: FBS Fetal Bovine Serum Source Type: news

Autophagy Assay Kits
Detects Autophagy in Living CellsAutophagy is a conserved lysosomal recycling process by which cells break down their own components such as proteins, lipids, and carbohydrates. The process plays an important role in maintaining homeostasis and destroying intracellular pathogens. In addition, autophagy can be upregulated in times of starvation or stress to provide additional nutrients for the cell. Dysregulation of autophagy has implications for cancer, infection, and degenerative diseases.Autophagy is a three-stage process. First, cytoplasmic components targeted for degradation are sequestered, resulting in the formation ...
Source: Neuromics - June 26, 2018 Category: Neuroscience Tags: Apoptosis Detection Apoptosis Visualization autophagy autophagy detection Cytotoxicity Assays necrosis Source Type: news

Markers for Tyrosine Hydroxylase-TH
Proven and PublishedTyrosine hydroxylase TH is a necessary enzyme to create neurotransmitters and protect the body against oxidative stress.Dysregulation of this enzyme results in manic-depression and schizophrenia. Parkinson's disease is also considered a TH deficiency as low dopamine levels are a consistent neurochemical abnormality.Markers to TH are important for studying its role in neurotransmission and how it is altered in disorders and diseases.We have many options and this enables you to choose the best markers for your research. All are proven andpublished.TH staining of mouse salivary gland tissue. https://d...
Source: Neuromics - June 15, 2018 Category: Neuroscience Tags: neuroscience TH antibody Tyrosine Hydroxlase antibody Source Type: news

Put our Antibodies to Work for You!
Tested and Characterized for Results You Can TrustWhen you select a vendor forantibodies, there is a measure of trust. We know you are asking, " will it work in the applications as advertised " .Since the inception of Neuromics, our guiding principle is providing well-characterized solutions for results you can trust and understand. In order to provide as much comfort in the purchase of our solutions, we encourage access topublications,data, andtestimonials.Here're some recent testimonials:Good antibody! Product Name: Tuj 1, Chicken – (Cat# CH23005) Good for neuronal staining. Bright and specific.Liang Shi ...
Source: Neuromics - June 6, 2018 Category: Neuroscience Tags: Antibodies Antibody TRPV1 antibodies TRPV1 antibody Source Type: news

New Fibrillarin Antibody
Potent Nucleoli MarkerGiven the importance ofNucleus and Related Markers to all branches of cell/molecular biology, we are dedicated to having the best.We have just added a newFibrillarin Antibody.Images: Immunofluorescent analysis of (A) C6 rat glioma cells and (B) Human embryonic kidney cells stained with mouse mAb to fibrillarin, MO22169, dilution 1:1,000 in red, in both cases costained with chicken pAb to vimentin, CPCA-Vim, dilution 1:10,000 in green. The blue is DAPI staining of nuclear DNA.Nop1p/Fibrillarin was originally identified as a nucleolar protein of bakers yeast, Saccharomyces cerevisiae (accession P15646)....
Source: Neuromics - June 4, 2018 Category: Neuroscience Tags: Fibrillarin Antibody Nuclear Staining Nucleoli Source Type: news

New 3-D Eye Model
Morein-vivo like ModelWe see our world in 3-D. Diseases of the eye compromise this ability.Neuromics'is pleased to announce that we have a3-D model aimed at accelerating drug discovery for these diseases. Sight is a terrible thing to lose and the faster new drugs can be discovered, fewer people will have to suffer the loss of sight.Our 3D Human Retinal Microvascular Angiogenesis model is constructed using GFP ‐Tagged human Retinal Microvascular Endothelial cells. They are co-cultured with RFP-Tagged human supporting cells. GFP positive human retinal capillary-like tubule formation can be monitored in real time under fluo...
Source: Neuromics - May 30, 2018 Category: Neuroscience Tags: 3-D Cell based Assays 3-D Cell Culturing 3-D Retinal Organoids Diabetic retinopathy Human Retinal Microvascular Endothelial Cells Source Type: news

Antibodies Against Neuropeptides
Another Nod From Nature Scientific ReportsPublications referencing use of our antibodies continue to build. We are recognized for having potentNeuropeptide Antibodies.This most recent publication references ourGuinea Pig Substance-P Antibody: Khaled Abdallah, Francis Nadeau, Francis Bergeron, Sylvie Blouin, V éronique Blais, Kelly M. Bradbury, Christine L. Lavoie, Jean-Luc Parent;, Louis Gendron. (2018). Adeno-associated virus 2/9 delivery of Cre recombinase in mouse primary afferents. Scientific Reports volume 8, Article number: 7321 (2018) doi:10.1038/s41598-018-25626-yFigure: Distribution of the Cre-GFP in t...
Source: Neuromics - May 21, 2018 Category: Neuroscience Tags: Neuropeptide Antibodies Neuropeptides Substance P Antibodies Substance P Antibody Source Type: news

Researchers Love our FBS
Potent Serum at a Great Price!We are pleased by the 5-star ratings on ourFetal Bovine Serum(FBS). Check them out.Outstanding service. Fetal bovine serum was of highest quality, easy to order and packaging and shipping were best possible.Rosemary Dziak - May 08, 2018 - Rating: 5.0This is first time to buy FBS from them. Rose is very nice person. they make you easy and comfortable to purchase.jie wei - Feb 16, 2018 - Rating: 5.0I enjoyed working with the people at Neuromics. The sale person was very friend and willing to take extra time to solve our problems. Product Name: FBS - (Cat#FBS001) https://www.neuromics.com/FBS001 ...
Source: Neuromics - May 10, 2018 Category: Neuroscience Tags: 3-D Cell based Assays Cell Based Assays. FBS Fetal Bovine Serum Source Type: news

Fibroblast Compression and Tumor Cells Migration
Role of Compression in MetastasisOurHuman Pancreatic Fibroblasts play a key role in this study.Pancreatic fibroblasts are continuously gaining ground as an important component of tumor microenvironment that dynamically interact with cancer cells to promote tumor progression. In addition, these tumor-infiltrated fibroblasts can acquire an activated phenotype and produce excessive amounts of extracellu lar matrix creating a highly dense stroma, a situation known as desmoplasia. Maria Kalli, Panagiotis Papageorgis, Vasiliki Gkretsi, Triantafyllos Stylianopoulos. (2018).Solid Stress Facilitates Fibroblasts Activation to Pro...
Source: Neuromics - May 1, 2018 Category: Neuroscience Tags: Cancer Associated Fibroblasts Desmoplasia Human Pancreatic Fibroblasts metastasis Source Type: news

Pure and Potent Human Primary Retinal Pigment Endothelial Cells (HRPES)
Culture ReadyNeuromics is a recognized leader in providing researchers 21-CFR CompliantPrimary Human Cells.Here we feature our newRetinal Pigment Epithelial Cell (HRPES). These hard to find cells are reasonably priced and culture ready,HRPES in CultureCells are provided at passage 3. HRPEs growth medium (contains 10% serum and growth supplements, Alpha-33) is recommended for cell culture and these cells have a minimum average population doubling capacity of 8 when cultured following the detailedprotocol. (Source: Neuromics)
Source: Neuromics - April 26, 2018 Category: Neuroscience Tags: cell culturing protocol culturing human retinal pigment epithelial cells Source Type: news

ATP and Pain
P2XRs Play a Key RoleIn dorsal root ganglion (DRG) neurons, ATP is an important neurotransmitter in nociceptive signaling through P2 receptors (P2Rs) such as P2X2/3R, and adenosine is also involved in anti-nociceptive signaling through adenosine A1R.ENNPs interact with P2XRs to metabolize ATP to AMP in DRGs. Kentaro Nishida, Yuka Nomura, Kanako Kawamori, Akihiro Ohishi, Kazuki Nagasawa.ATP metabolizing enzymes ENPP1, 2 and 3 are localized in sensory neurons of rat dorsal root ganglion. European Journal of Histochemistry 2018; 62:2877.Images: Rat DRG stained withNeuromics P2X2Rand ENPP1.Modulating ATP Processing in DRG...
Source: Neuromics - April 19, 2018 Category: Neuroscience Source Type: news

Long Term Culturing of Cells
Non-Perfusion ModelResearchers, for some studies, are demanding the ability to maintain vibrant cultures for long periods of time.Perfusion models afford this, but are expensive, sensitive and require specialized expertise. Given this, I believed it a good time to represent a protocol that can be used for ourprimary neurons and can be extrapolated to many of our other primary and stem cells.Here's the complete protocol.Rose Ludescher, Manager of Customer Satisfaction, is an expert in helping our customers successfully culturing cells. So if you need help with any of your cell-based assays do not hesitate to contact her. Ju...
Source: Neuromics - April 14, 2018 Category: Neuroscience Tags: Cell based assays Cell Cultures cell culturing protocol Neurons Source Type: news

National Eye Institute's 3-D ROC Challenge
Are you Ready?Neuromics is a proudSponsor of this challenge. It enables us to further leverage our potent, proven and published3-D Cell-Based Assay Solutions into drug discovery for eye-related diseases.Our solution set includes 21-CFR Complianthuman primary and stem cells and research ready custom and off the shelf 3-D Models. We also provide defined media and supplements.Neuromics 3-D Blood-Brain Barrier ModelIn addition to models, we offer: ECMS•Engineered hydrogels optimized for cell types•Coming soon: Bio-Inks for 3-D Printing-Engineered for Cells•NanofibersNeuromics ’ HUVECS in an engin...
Source: Neuromics - April 7, 2018 Category: Neuroscience Tags: 3-D Assays. 3-D imaging 3-D Cell based Assays 3-D ROC Challenge Collagen Hydrogels National Eye Insititute Source Type: news